Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0000377

Physical Location & Seq

Genomic Coordinates Forward (+)

51206723 .. 51213379

Gene Structure 6657 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003644

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2607 bp

ATGACACTCTCTTATGAATTCTTCATGGAATCCAATTCCTTATCTTCACTGTGGTCGGAAACCAGCAAGGGGAATTGGGGAGTGTTGTTCACTATCTCCGCAATTTTTGCAGCAATTTTTTACGCATGGAGTTGCCTGGAGAGTTGCAGAATCTCGCCGCCGTTGCCTCCAGGTCCACGTGGCCTCCCGCTGGTTGGTAATCTTCTATCCGTTGACCCACAGCTCCACACCTACTTTGCGGGCCTATCCAAAACTTACGGCCCAATCTATAAGCTCCGACTGGGTACCAGGCTCTGTGTCGTAATAGCCTCCCCTTCCGCCGCTCGAGAGGTGCTCAAGGATAATGACCTCACGTTCTCCAGTCGCGGCGTCAATTGTGCAGCTGGGGACGTCGCACTCTACGGCGGGGCAGGTATAGTTTGTACCCCGTATGGGCCGGAGTGGCGAATGTTGAGGAAAGTATGCGTGCTGAAGATGCTTAGCAACACCGCCTTGGACTCGGTTCACCAACTGCGCCACTCGCAGGTCCGAAAAGCTGTGCGTTACTTCTACAGTCAGGTTGGGTCGCCCGTCAACATTGGTGAGCAACTTTTCGTGGCCACGCTCAATCTTACCACCAACATGTTATGGGGTGGCACCATGCTGGAGGAGGAGGCTGGGGCTGAGGTTTTGGCGGAGTTTAGGGAAGCGATGGTCGACATCGCGGTGCTTTCTGGGAAACCGAATATTTCGGAGCTTTATCCGTTTTTGACCCGGTTTGATGTGCAAGGCATAAGGAAGCAGATGAAGGCTGTGGAGCTCAAGTTTGATAGCATTTTTGAGAAAGTTATTGATCAACGGTTGAAAATGGACAAACAAGGCGCGCAAGAGAGCAAGGATTTCTTGACATTTATGTTGCGATATAAAGACGAAGGAGGAGATGCCAGAACTCCTCTCACCATGACCCATCTCAAAGCCTTGCTCTTGGACATGGTTGTGGGTGGGAGTGACACATCCTCCAACACAATTGAGTTTGCAATGGCTGAAATGATGAACAAACCAGAAGTGATAGAGAAAGCCCAGAAAGAATTAGACGCTGTAGTTGGAAAAGACAGTATCGTACAAGAGTCTCATATTCACAAACTGCCATACTTGGAAGCTGTGATGAAAGAAACTCTGCGCTTGCACCCAGCCCTGCCTCTAATGCTCCCACATTGCCCAAGTGAAACATGCATTGTGGGAGGTTATACCATTCCAAAGGGGTCTAAGGTTTTTGTTAATGTGTGGGATATCCACAGAGACCCTTCTAACTGGGAAGACCCATTAGAGTTTAATCCAGAGAGGTTCTTGGATGGTAAATATGACTTCACTGGAAGCAACTTCAACTACTTTCCATTTGGGAGTGGAAAAAGAATATGTCCAGGGACAGCAACGGCAGAGAGGCTTGTCATGCATTTACTTGCATCGCTGTTGCATTCTTTTGACTGGAAACTACCACAAGGAGAGACAAAGGTGGATTCTTCCGAAAAGTTTGGGATGGTGATGATGAAGAAGGTACCTATGGTTGCCATCCCAACTCCAAGGTTATTGGATTCAGATCTTTATCATGAGGTGGAGCAACTTGAGGGATTGGAGAAAGAGAGGTGGTTTGGGTTGACTCGTCGGAGGAAGAAGGTCGAACAAGAGCTATGCATAGAGTCGGATGAGCAAAGAGGAAGAAGGTCGGGTAGCCAGAGGAGAAGTGGGTGTCCAAATCAATTATGGCTTTTGAACAAGGTGATTGCAGAAAAGCTTGACCTAGAAATCTTGTCGTCTCCCAGCTCCTTTCACATTGCAGATCTGGGTTGCTCTGTTGGGCCGAATACATTTTCTGCAGTTGAAAACATAATTGAAGCCGTGGTACTCAAGTATCACAGCCAGCTTGGTTTGAATGTTCAAATCCCTGAATTTCAAGTCTTCTTCAATGATCATACCTCAAATGACTTCAACCTGCTCTTCAAATCCCTCCCTCAGAACAGGCAATACTACGCTGCCGGTGTACCGGGTTCTTTTTACGATCGCAGATTTCCTGAGGCTTCCCTTCACTTGGTTCACTCTTCTACTGCCCTTCACTGGCTTTCTAAAGTACCAGCGGAGGTGTTGGACAAAAACAGTCTTGCTTATAACAAAGGACGAATCCATTACTCAAATGCCACAGATGAAGTGATAAGGGCTCATGAAACTCAATATGCGGTAGACATGGAGCGCTTTCTCCATTCCCGGGCACAAGAGGTTGTTTATGGAGGACTCATGGGAGTTGTTAGTGAAGAGAAAGTAGATTCGTTTAACATACCTACGTATACAATGTCTCCCCAAGAACTGAAAGCTGTTGTAGAACGAAATGGATGTTTTAGCATAGAGACAATAACACAGTTACCTCTTGTAGTCTTGGCAGATAGGCCTAATATGGCACTGCCGCAACTACTTGCCGCTCATATGAGAGCTGGCACGGAAGGGATAGTCAAGGAGCACTTTGGGGAAGAAATATTAGATGAGCTCTTCGACTTGTATCTCACAAAATGTGAAGAGCATGTCACTGCTGTTGAGACAGTTACATTTCTTGTTGTGCTTAAACGCAAGGCAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0016020 GO:0019748 GO:0042221 GO:0044550 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko00140 ko01100 ko04913 ko04917 ko04927 ko04934 map00140 map01100 map04913 map04917 map04927 map04934

Pfam Domains

Protein Families

p450

Protein Analysis

869

Amino Acids

97.49

Weight (kDa)

5.94

Isoelectric Point (pI)

45.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	56 - 512	4.6e-103	Cytochrome P450
Methyltransf_7	PF03492	593 - 757	2.6e-65	SAM dependent carboxyl methyltransferase
Methyltransf_7	PF03492	758 - 865	2.9e-21	SAM dependent carboxyl methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000377)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G12300 AT4G12310 AT4G12320 AT4G12330 AT4G22690 AT4G22710 AT5G44620
fragaria_vesca	FvH4_3g36340 FvH4_4g05231 FvH4_5g00500 FvH4_5g00510 FvH4_7g17980
malus_domestica	MD02G1257600.v1.1 MD04G1080300.v1.1 MD06G1075700.v1.1
prunus_persica	Prupe.1G090700_v2.0.a1 Prupe.5G071500_v2.0.a1 Prupe.5G077600_v2.0.a1 Prupe.5G077700_v2.0.a1 Prupe.5G077800_v2.0.a1 Prupe.5G077900_v2.0.a1 Prupe.5G078100_v2.0.a1 Prupe.5G078200_v2.0.a1 Prupe.5G078300_v2.0.a1
pyrus_communis	pycom04g07220 pycom04g07240 pycom06g05480 pycom15g15400
rosa_chinensis	RchiOBHm_Chr5g0018741 RchiOBHm_Chr6g0248821 RchiOBHm_Chr7g0178901 RchiOBHm_Chr7g0201891 RchiOBHm_Chr7g0201961 RchiOBHm_Chr7g0201971 RchiOBHm_Chr7g0201981 RchiOBHm_Chr7g0201991 RchiOBHm_Chr7g0202001
rosa_laevigata	RLG00000003634 RLG00000003636 RLG00000003637 RLG00000003638 RLG00000003644
rosa_multiflora	Rmu_co8316029.1_g000001 Rmu_sc0000637.1_g000020 Rmu_sc0000637.1_g000025 Rmu_sc0000637.1_g000027 Rmu_sc0001030.1_g000027 Rmu_sc0006420.1_g000002 Rmu_sc0012276.1_g000002 Rmu_sc0012276.1_g000005 Rmu_sc0012276.1_g000011 Rmu_sc0012276.1_g000012 Rmu_sc0012276.1_g000017 Rmu_sc0014024.1_g000001 Rmu_ssc0000125.1_g000026
rosa_roxburghii	Rroxscaffold_2G00077750 Rroxscaffold_2G00113690 Rroxscaffold_3G00254960 Rroxscaffold_3G00254970 Rroxscaffold_3G00254980 Rroxscaffold_3G00255010 Rroxscaffold_3G00255020 Rroxscaffold_5G00357760 Rroxscaffold_5G00357810
rosa_rugosa	Rorug05G0288400 Rorug05G0288500 Rorug07G0066500 Rorug07G0066700 Rorug07G0066800 Rorug07G0066800 Rorug07G0066900 Rorug07G0066900 Rorug07G0067000 Rorug07G0067100 Rorug07G0067200.1
rosa_samantha	Rh3DG308900 Rh4AG183200 Rh4CG451700 Rh5BG064200 Rh7AG193800 Rh7AG194000 Rh7AG194300 Rh7AG194400 Rh7BG195400 Rh7BG195600 Rh7BG195700 Rh7BG195800 Rh7BG195900 Rh7CG205300 Rh7CG205500 Rh7CG205600 Rh7CG205700 Rh7DG020100 Rh7DG199900 Rh7DG200600 Rh7DG200700 Rh7DG200900 Rh7DG201000 Rh7DG201100
rosa_wichuraiana	Rw7G016940 Rw7G016980 Rw7G016990 Rw7G017010 Rw7G017020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2142
AatII	GACGTC	1 cut(s)	393
Acc36I	ACCTGC	3 cut(s)	401, 514, 1977
Acc65I	GGTACC	2 cut(s)	284, 1534
AccB1I	GGYRCC	3 cut(s)	284, 635, 1534
AccBSI	CCGCTC	2 cut(s)	323, 2450
AccI	GTMKAC	3 cut(s)	696, 2214, 2318
AccII	CGCG	3 cut(s)	366, 704, 863
AcoI	YGGCCR	1 cut(s)	597
AcsI	RAATTY	2 cut(s)	17, 1925
AcuI	CTGAAG	1 cut(s)	491
AcvI	CACGTG	1 cut(s)	179
AcyI	GRCGYC	2 cut(s)	369, 390
AfaI	GTAC	7 cut(s)	286, 424, 1101, 1536, 1881, 2019, 2106
AfeI	AGCGCT	1 cut(s)	2225
AfiI	CCNNNNNNNGG	8 cut(s)	69, 190, 194, 432, 523, 2065, 2091, 2239
AflIII	ACRYGT	1 cut(s)	621
AjnI	CCWGG	4 cut(s)	135, 169, 287, 1399
Alw21I	GWGCWC	4 cut(s)	336, 801, 2490, 2517
Alw26I	GTCTC	7 cut(s)	1113, 1272, 1478, 1797, 2331, 2372, 2558
AlwNI	CAGNNNCTG	1 cut(s)	294
Ama87I	CYCGRG	2 cut(s)	324, 2238
Aor51HI	AGCGCT	1 cut(s)	2225
AoxI	GGCC	7 cut(s)	181, 241, 259, 434, 597, 1835, 2417
ApeKI	GCWGC	3 cut(s)	110, 380, 2009
ApoI	RAATTY	2 cut(s)	17, 1925
Asp718I	GGTACC	2 cut(s)	284, 1534
AspLEI	GCGC	5 cut(s)	516, 863, 865, 1161, 2226
AspS9I	GGNCC	6 cut(s)	173, 241, 260, 434, 526, 1835
AsuC2I	CCSGG	4 cut(s)	754, 2022, 2239, 2240
AsuHPI	GGTGA	5 cut(s)	497, 593, 928, 1531, 1768
AvaI	CYCGRG	2 cut(s)	324, 2238
AvaII	GGWCC	2 cut(s)	173, 526
AxyI	CCTNAGG	1 cut(s)	2049
BaeGI	GKGCMC	1 cut(s)	2245
BaeI	ACNNNNGTAYC	4 cut(s)	1871, 1871, 1904, 1904
BalI	TGGCCA	1 cut(s)	599
BanI	GGYRCC	3 cut(s)	284, 635, 1534
BanII	GRGCYC	3 cut(s)	801, 2194, 2517
BarI	GAAGNNNNNNTAC	2 cut(s)	1863, 1895
BbrPI	CACGTG	1 cut(s)	179
BbsI	GAAGAC	2 cut(s)	1302, 1927
Bbv12I	GWGCWC	4 cut(s)	336, 801, 2490, 2517
BbvCI	CCTCAGC	1 cut(s)	663
BbvI	GCAGC	3 cut(s)	122, 392, 1996
BccI	CCATC	5 cut(s)	685, 954, 1325, 1510, 1556
BceAI	ACGGC	5 cut(s)	145, 274, 418, 1428, 1859
BciT130I	CCWGG	4 cut(s)	137, 171, 289, 1401
BclI	TGATCA	2 cut(s)	832, 1945
BcnI	CCSGG	4 cut(s)	754, 2022, 2239, 2240
BcoDI	GTCTC	7 cut(s)	1113, 1272, 1478, 1797, 2331, 2372, 2558
BfaI	CTAG	1 cut(s)	1778
BfmI	CTRYAG	3 cut(s)	550, 1077, 1851
BfoI	RGCGCY	1 cut(s)	2227
BfuAI	ACCTGC	3 cut(s)	401, 514, 1977
BglI	GCCNNNNNGGC	1 cut(s)	442
BglII	AGATCT	2 cut(s)	1576, 1816
BisI	GCNGC	8 cut(s)	111, 158, 321, 367, 381, 2010, 2435, 2448
BlpI	GCTNAGC	1 cut(s)	479
BlsI	GCNGC	8 cut(s)	112, 159, 322, 368, 382, 2011, 2436, 2449
Bme1390I	CCNGG	8 cut(s)	137, 171, 289, 754, 1401, 2022, 2239, 2240
Bme18I	GGWCC	2 cut(s)	173, 526
BmeT110I	CYCGRG	2 cut(s)	324, 2238
BmgT120I	GGNCC	6 cut(s)	173, 241, 260, 434, 526, 1835
BmiI	GGNNCC	3 cut(s)	286, 637, 1536
BmrFI	CCNGG	8 cut(s)	137, 171, 289, 754, 1401, 2022, 2239, 2240
BmrI	ACTGGG	2 cut(s)	290, 1300
BmsI	GCATC	3 cut(s)	465, 910, 1451
BmuI	ACTGGG	2 cut(s)	290, 1300
BpiI	GAAGAC	2 cut(s)	1302, 1927
BplI	GAGNNNNNCTC	2 cut(s)	318, 350
BpmI	CTGGAG	4 cut(s)	153, 158, 343, 665
Bpu10I	CCTNAGC	1 cut(s)	663
Bpu1102I	GCTNAGC	1 cut(s)	479
BpuEI	CTTGAG	4 cut(s)	320, 785, 1622, 1868
BpuMI	CCSGG	4 cut(s)	754, 2022, 2239, 2240
BsaAI	YACGTR	2 cut(s)	179, 2316
BsaBI	GATNNNNATC	2 cut(s)	1575, 1581
BsaHI	GRCGYC	2 cut(s)	369, 390
BsaI	GGTCTC	1 cut(s)	1272
BsaJI	CCNNGG	5 cut(s)	492, 1400, 1559, 1875, 2238
BsaXI	ACNNNNNCTCC	8 cut(s)	168, 198, 788, 818, 980, 1010, 2311, 2341
Bsc4I	CCNNNNNNNGG	8 cut(s)	69, 190, 194, 432, 523, 2065, 2091, 2239
Bse118I	RCCGGY	1 cut(s)	2012
Bse1I	ACTGG	6 cut(s)	285, 360, 1295, 1354, 1469, 2096
Bse21I	CCTNAGG	1 cut(s)	2049
Bse3DI	GCAATG	3 cut(s)	1023, 1192, 1809
Bse8I	GATNNNNATC	2 cut(s)	1575, 1581
BseBI	CCWGG	4 cut(s)	137, 171, 289, 1401
BseDI	CCNNGG	5 cut(s)	492, 1400, 1559, 1875, 2238
BseGI	GGATG	6 cut(s)	992, 1336, 1521, 1548, 1687, 2369
BseJI	GATNNNNATC	2 cut(s)	1575, 1581
BseLI	CCNNNNNNNGG	8 cut(s)	69, 190, 194, 432, 523, 2065, 2091, 2239
BseMI	GCAATG	3 cut(s)	1023, 1192, 1809
BseMII	CTCAG	3 cut(s)	654, 2003, 2040
BseNI	ACTGG	6 cut(s)	285, 360, 1295, 1354, 1469, 2096
BsePI	GCGCGC	1 cut(s)	861
BseRI	GAGGAG	5 cut(s)	662, 665, 921, 930, 1729
BseSI	GKGCMC	1 cut(s)	2245
BseXI	GCAGC	3 cut(s)	122, 392, 1996
BseYI	CCCAGC	4 cut(s)	383, 656, 1168, 1796
BsgI	GTGCAG	1 cut(s)	399
Bsh1236I	CGCG	3 cut(s)	366, 704, 863
Bsh1285I	CGRYCG	1 cut(s)	2038
BshFI	GGCC	7 cut(s)	183, 243, 261, 436, 599, 1837, 2419
BshNI	GGYRCC	3 cut(s)	284, 635, 1534
BsiEI	CGRYCG	1 cut(s)	2038
BsiHKAI	GWGCWC	4 cut(s)	336, 801, 2490, 2517
BsiHKCI	CYCGRG	2 cut(s)	324, 2238
BsiSI	CCGG	5 cut(s)	437, 754, 2013, 2021, 2239
BslFI	GGGAC	2 cut(s)	401, 1417
BslI	CCNNNNNNNGG	8 cut(s)	69, 190, 194, 432, 523, 2065, 2091, 2239
BsmAI	GTCTC	7 cut(s)	1113, 1272, 1478, 1797, 2331, 2372, 2558
BsmBI	CGTCTC	1 cut(s)	1797
BsmFI	GGGAC	2 cut(s)	401, 1417
BsmI	GAATGC	1 cut(s)	1453
BsnI	GGCC	7 cut(s)	183, 243, 261, 436, 599, 1837, 2419
Bso31I	GGTCTC	1 cut(s)	1272
BsoBI	CYCGRG	2 cut(s)	324, 2238
Bsp1286I	GDGCHC	6 cut(s)	336, 801, 2194, 2245, 2490, 2517
Bsp143I	GATC	5 cut(s)	832, 1576, 1816, 1945, 2035
Bsp1720I	GCTNAGC	1 cut(s)	479
BspANI	GGCC	7 cut(s)	183, 243, 261, 436, 599, 1837, 2419
BspCNI	CTCAG	3 cut(s)	655, 2002, 2041
BspFNI	CGCG	3 cut(s)	366, 704, 863
BspHI	TCATGA	2 cut(s)	1585, 2194
BspLI	GGNNCC	3 cut(s)	286, 637, 1536
BspMAI	CTGCAG	1 cut(s)	1855
BspMI	ACCTGC	3 cut(s)	401, 514, 1977
BspQI	GCTCTTC	3 cut(s)	1979, 2522, 2538
BspT107I	GGYRCC	3 cut(s)	284, 635, 1534
BspTNI	GGTCTC	1 cut(s)	1272
BsrBI	CCGCTC	2 cut(s)	323, 2450
BsrDI	GCAATG	3 cut(s)	1023, 1192, 1809
BsrFI	RCCGGY	1 cut(s)	2012
BsrI	ACTGG	6 cut(s)	285, 360, 1295, 1354, 1469, 2096
BssAI	RCCGGY	1 cut(s)	2012
BssECI	CCNNGG	5 cut(s)	492, 1400, 1559, 1875, 2238
BssHII	GCGCGC	1 cut(s)	861
BssMI	GATC	5 cut(s)	832, 1576, 1816, 1945, 2035
BssNAI	GTATAC	1 cut(s)	2319
BssNI	GRCGYC	2 cut(s)	369, 390
BssT1I	CCWWGG	2 cut(s)	492, 1559
Bst1107I	GTATAC	1 cut(s)	2319
Bst2UI	CCWGG	4 cut(s)	137, 171, 289, 1401
Bst4CI	ACNGT	7 cut(s)	51, 554, 840, 1094, 2132, 2391, 2569
Bst6I	CTCTTC	5 cut(s)	1979, 2080, 2280, 2522, 2538
BstACI	GRCGYC	2 cut(s)	369, 390
BstAPI	GCANNNNNTGC	1 cut(s)	107
BstBAI	YACGTR	2 cut(s)	179, 2316
BstC8I	GCNNGC	6 cut(s)	241, 467, 863, 1163, 1898, 2464
BstDEI	CTNAG	5 cut(s)	479, 663, 1245, 1989, 2049
BstDSI	CCRYGG	1 cut(s)	1875
BstF5I	GGATG	6 cut(s)	992, 1336, 1521, 1548, 1687, 2369
BstFNI	CGCG	3 cut(s)	366, 704, 863
BstH2I	RGCGCY	1 cut(s)	2227
BstHHI	GCGC	5 cut(s)	516, 863, 865, 1161, 2226
BstKTI	GATC	5 cut(s)	835, 1579, 1819, 1948, 2038
BstMAI	GTCTC	7 cut(s)	1113, 1272, 1478, 1797, 2331, 2372, 2558
BstMBI	GATC	5 cut(s)	832, 1576, 1816, 1945, 2035
BstMCI	CGRYCG	1 cut(s)	2038
BstMWI	GCNNNNNNNGC	7 cut(s)	107, 163, 442, 475, 520, 1183, 1429
BstNI	CCWGG	4 cut(s)	137, 171, 289, 1401
BstNSI	RCATGY	3 cut(s)	625, 1212, 2552
BstSCI	CCNGG	8 cut(s)	135, 169, 287, 752, 1399, 2020, 2237, 2238
BstSFI	CTRYAG	3 cut(s)	550, 1077, 1851
BstSLI	GKGCMC	1 cut(s)	2245
BstSNI	TACGTA	1 cut(s)	2316
BstUI	CGCG	3 cut(s)	366, 704, 863
BstV1I	GCAGC	3 cut(s)	122, 392, 1996
BstV2I	GAAGAC	2 cut(s)	1302, 1927
BstX2I	RGATCY	2 cut(s)	1576, 1816
BstYI	RGATCY	2 cut(s)	1576, 1816
BstZ17I	GTATAC	1 cut(s)	2319
Bsu36I	CCTNAGG	1 cut(s)	2049
BsuRI	GGCC	7 cut(s)	183, 243, 261, 436, 599, 1837, 2419
BtgI	CCRYGG	1 cut(s)	1875
BtgZI	GCGATG	3 cut(s)	685, 704, 1428
BtsCI	GGATG	6 cut(s)	992, 1336, 1521, 1548, 1687, 2369
BtsI	GCAGTG	2 cut(s)	2429, 2553
BtsIMutI	CAGTG	5 cut(s)	47, 1347, 2089, 2429, 2553
BveI	ACCTGC	3 cut(s)	401, 514, 1977
Cac8I	GCNNGC	6 cut(s)	241, 467, 863, 1163, 1898, 2464
CaiI	CAGNNNCTG	1 cut(s)	294
CciI	TCATGA	2 cut(s)	1585, 2194
CfoI	GCGC	5 cut(s)	516, 863, 865, 1161, 2226
Cfr10I	RCCGGY	1 cut(s)	2012
Cfr13I	GGNCC	6 cut(s)	173, 241, 260, 434, 526, 1835
Cfr9I	CCCGGG	1 cut(s)	2238
CseI	GACGC	2 cut(s)	358, 1082
Csp6I	GTAC	7 cut(s)	285, 423, 1100, 1535, 1880, 2018, 2105
CviQI	GTAC	7 cut(s)	285, 423, 1100, 1535, 1880, 2018, 2105
DdeI	CTNAG	5 cut(s)	479, 663, 1245, 1989, 2049
DpnI	GATC	5 cut(s)	834, 1578, 1818, 1947, 2037
DpnII	GATC	5 cut(s)	832, 1576, 1816, 1945, 2035
EaeI	YGGCCR	1 cut(s)	597
Eam1104I	CTCTTC	5 cut(s)	1979, 2080, 2280, 2522, 2538
EarI	CTCTTC	5 cut(s)	1979, 2080, 2280, 2522, 2538
EciI	GGCGGA	2 cut(s)	307, 689
Ecl136II	GAGCTC	2 cut(s)	799, 2515
Eco105I	TACGTA	1 cut(s)	2316
Eco130I	CCWWGG	2 cut(s)	492, 1559
Eco147I	AGGCCT	1 cut(s)	2419
Eco24I	GRGCYC	3 cut(s)	801, 2194, 2517
Eco31I	GGTCTC	1 cut(s)	1272
Eco32I	GATATC	1 cut(s)	1270
Eco47I	GGWCC	2 cut(s)	173, 526
Eco47III	AGCGCT	1 cut(s)	2225
Eco53kI	GAGCTC	2 cut(s)	799, 2515
Eco57I	CTGAAG	1 cut(s)	491
Eco72I	CACGTG	1 cut(s)	179
Eco81I	CCTNAGG	1 cut(s)	2049
Eco88I	CYCGRG	2 cut(s)	324, 2238
EcoICRI	GAGCTC	2 cut(s)	799, 2515
EcoRI	GAATTC	1 cut(s)	17
EcoRII	CCWGG	4 cut(s)	135, 169, 287, 1399
EcoRV	GATATC	1 cut(s)	1270
EcoT14I	CCWWGG	2 cut(s)	492, 1559
EcoT22I	ATGCAT	3 cut(s)	1214, 1434, 1673
EcoT38I	GRGCYC	3 cut(s)	801, 2194, 2517
ErhI	CCWWGG	2 cut(s)	492, 1559
Esp3I	CGTCTC	1 cut(s)	1797
FalI	AAGNNNNNCTT	4 cut(s)	866, 898, 2474, 2506
FaqI	GGGAC	2 cut(s)	401, 1417
FauI	CCCGC	3 cut(s)	195, 232, 398
FauNDI	CATATG	1 cut(s)	2454
FbaI	TGATCA	2 cut(s)	832, 1945
FblI	GTMKAC	3 cut(s)	696, 2214, 2318
Fnu4HI	GCNGC	8 cut(s)	111, 158, 321, 367, 381, 2010, 2435, 2448
FokI	GGATG	6 cut(s)	979, 1343, 1528, 1535, 1694, 2376
FriOI	GRGCYC	3 cut(s)	801, 2194, 2517
Fsp4HI	GCNGC	8 cut(s)	111, 158, 321, 367, 381, 2010, 2435, 2448
FspBI	CTAG	1 cut(s)	1778
GlaI	GCGC	5 cut(s)	515, 862, 864, 1160, 2225
GluI	GCNGC	8 cut(s)	111, 158, 321, 367, 381, 2010, 2435, 2448
GsaI	CCCAGC	4 cut(s)	387, 660, 1172, 1800
GsuI	CTGGAG	4 cut(s)	153, 158, 343, 665
HaeII	RGCGCY	1 cut(s)	2227
HaeIII	GGCC	7 cut(s)	183, 243, 261, 436, 599, 1837, 2419
HapII	CCGG	5 cut(s)	437, 754, 2013, 2021, 2239
HgaI	GACGC	2 cut(s)	358, 1082
HhaI	GCGC	5 cut(s)	516, 863, 865, 1161, 2226
Hin1I	GRCGYC	2 cut(s)	369, 390
Hin6I	GCGC	5 cut(s)	514, 861, 863, 1159, 2224
HinP1I	GCGC	5 cut(s)	514, 861, 863, 1159, 2224
HincII	GTYRAC	4 cut(s)	214, 574, 697, 1635
HindII	GTYRAC	4 cut(s)	214, 574, 697, 1635
HindIII	AAGCTT	1 cut(s)	1769
HpaII	CCGG	5 cut(s)	437, 754, 2013, 2021, 2239
HphI	GGTGA	5 cut(s)	497, 593, 928, 1531, 1768
Hpy188I	TCNGA	9 cut(s)	58, 278, 530, 733, 1504, 1576, 1644, 1681, 1992
Hpy188III	TCNNGA	6 cut(s)	326, 883, 1316, 1586, 2048, 2195
Hpy99I	CGWCG	2 cut(s)	395, 1644
HpyCH4III	ACNGT	7 cut(s)	51, 554, 840, 1094, 2132, 2391, 2569
HpyCH4IV	ACGT	4 cut(s)	178, 353, 390, 2315
HpyF10VI	GCNNNNNNNGC	7 cut(s)	107, 163, 442, 475, 520, 1183, 1429
HpyF3I	CTNAG	5 cut(s)	479, 663, 1245, 1989, 2049
HpySE526I	ACGT	4 cut(s)	178, 353, 390, 2315
Hsp92I	GRCGYC	2 cut(s)	369, 390
HspAI	GCGC	5 cut(s)	514, 861, 863, 1159, 2224
KpnI	GGTACC	2 cut(s)	288, 1538
Ksp22I	TGATCA	2 cut(s)	832, 1945
Kzo9I	GATC	5 cut(s)	832, 1576, 1816, 1945, 2035
LguI	GCTCTTC	3 cut(s)	1979, 2522, 2538
LmnI	GCTCC	9 cut(s)	228, 279, 733, 796, 1191, 1594, 1805, 2221, 2485
Lsp1109I	GCAGC	3 cut(s)	122, 392, 1996
LweI	GCATC	3 cut(s)	465, 910, 1451
MaeI	CTAG	1 cut(s)	1778
MaeII	ACGT	4 cut(s)	178, 353, 390, 2315
MaeIII	GTNAC	5 cut(s)	542, 986, 2391, 2551, 2569
MalI	GATC	5 cut(s)	834, 1578, 1818, 1947, 2037
MbiI	CCGCTC	2 cut(s)	323, 2450
MboI	GATC	5 cut(s)	832, 1576, 1816, 1945, 2035
MfeI	CAATTG	2 cut(s)	373, 1005
MflI	RGATCY	2 cut(s)	1576, 1816
MhlI	GDGCHC	6 cut(s)	336, 801, 2194, 2245, 2490, 2517
MlsI	TGGCCA	1 cut(s)	599
MluNI	TGGCCA	1 cut(s)	599
MlyI	GAGTC	5 cut(s)	491, 1115, 1630, 1685, 2259
MmeI	TCCRAC	7 cut(s)	36, 301, 1023, 1063, 1622, 1659, 2100
Mox20I	TGGCCA	1 cut(s)	599
Mph1103I	ATGCAT	3 cut(s)	1214, 1434, 1673
MscI	TGGCCA	1 cut(s)	599
MseI	TTAA	4 cut(s)	1257, 1311, 2304, 2589
MslI	CAYNNNNRTG	3 cut(s)	620, 704, 974
Msp20I	TGGCCA	1 cut(s)	599
MspA1I	CMGCKG	3 cut(s)	190, 383, 2111
MspI	CCGG	5 cut(s)	437, 754, 2013, 2021, 2239
MspR9I	CCNGG	8 cut(s)	137, 171, 289, 754, 1401, 2022, 2239, 2240
MunI	CAATTG	2 cut(s)	373, 1005
Mva1269I	GAATGC	1 cut(s)	1453
MvaI	CCWGG	4 cut(s)	137, 171, 289, 1401
MvnI	CGCG	3 cut(s)	366, 704, 863
MwoI	GCNNNNNNNGC	7 cut(s)	107, 163, 442, 475, 520, 1183, 1429
NciI	CCSGG	4 cut(s)	754, 2022, 2239, 2240
NdeI	CATATG	1 cut(s)	2454
NdeII	GATC	5 cut(s)	832, 1576, 1816, 1945, 2035
NlaIV	GGNNCC	3 cut(s)	286, 637, 1536
NmuCI	GTSAC	2 cut(s)	986, 2551
NsiI	ATGCAT	3 cut(s)	1214, 1434, 1673
NspI	RCATGY	3 cut(s)	625, 1212, 2552
PaeR7I	CTCGAG	1 cut(s)	324
PagI	TCATGA	2 cut(s)	1585, 2194
PauI	GCGCGC	1 cut(s)	861
PceI	AGGCCT	1 cut(s)	2419
PciI	ACATGT	1 cut(s)	621
PciSI	GCTCTTC	3 cut(s)	1979, 2522, 2538
PctI	GAATGC	1 cut(s)	1453
PfeI	GAWTC	6 cut(s)	29, 150, 1496, 1571, 2154, 2297
PkrI	GCNGC	8 cut(s)	112, 159, 322, 368, 382, 2011, 2436, 2449
Ple19I	CGATCG	1 cut(s)	2038
PleI	GAGTC	5 cut(s)	491, 1114, 1630, 1684, 2259
PmaCI	CACGTG	1 cut(s)	179
PmlI	CACGTG	1 cut(s)	179
PpsI	GAGTC	5 cut(s)	491, 1114, 1630, 1684, 2259
Ppu21I	YACGTR	2 cut(s)	179, 2316
PscI	ACATGT	1 cut(s)	621
PsiI	TTATAA	1 cut(s)	2142
Psp124BI	GAGCTC	2 cut(s)	801, 2517
Psp6I	CCWGG	4 cut(s)	135, 169, 287, 1399
PspCI	CACGTG	1 cut(s)	179
PspFI	CCCAGC	4 cut(s)	383, 656, 1168, 1796
PspGI	CCWGG	4 cut(s)	135, 169, 287, 1399
PspN4I	GGNNCC	3 cut(s)	286, 637, 1536
PspPI	GGNCC	6 cut(s)	173, 241, 260, 434, 526, 1835
PsrI	GAACNNNNNNTAC	2 cut(s)	1985, 2017
PstI	CTGCAG	1 cut(s)	1855
PstNI	CAGNNNCTG	1 cut(s)	294
PsuI	RGATCY	2 cut(s)	1576, 1816
PteI	GCGCGC	1 cut(s)	861
PvuI	CGATCG	1 cut(s)	2038
PvuII	CAGCTG	1 cut(s)	383
RsaI	GTAC	7 cut(s)	286, 424, 1101, 1536, 1881, 2019, 2106
RsaNI	GTAC	7 cut(s)	285, 423, 1100, 1535, 1880, 2018, 2105
RseI	CAYNNNNRTG	3 cut(s)	620, 704, 974
SacI	GAGCTC	2 cut(s)	801, 2517
SalI	GTCGAC	1 cut(s)	695
SapI	GCTCTTC	3 cut(s)	1979, 2522, 2538
SaqAI	TTAA	4 cut(s)	1257, 1311, 2304, 2589
SatI	GCNGC	8 cut(s)	111, 158, 321, 367, 381, 2010, 2435, 2448
Sau3AI	GATC	5 cut(s)	832, 1576, 1816, 1945, 2035
Sau96I	GGNCC	6 cut(s)	173, 241, 260, 434, 526, 1835
SchI	GAGTC	5 cut(s)	491, 1115, 1630, 1685, 2259
ScrFI	CCNGG	8 cut(s)	137, 171, 289, 754, 1401, 2022, 2239, 2240
SduI	GDGCHC	6 cut(s)	336, 801, 2194, 2245, 2490, 2517
SfaNI	GCATC	3 cut(s)	465, 910, 1451
SfcI	CTRYAG	3 cut(s)	550, 1077, 1851
Sfr274I	CTCGAG	1 cut(s)	324
SinI	GGWCC	2 cut(s)	173, 526
SlaI	CTCGAG	1 cut(s)	324
SmaI	CCCGGG	1 cut(s)	2240
SmiMI	CAYNNNNRTG	3 cut(s)	620, 704, 974
SmlI	CTYRAG	5 cut(s)	324, 335, 800, 1601, 1883
SmoI	CTYRAG	5 cut(s)	324, 335, 800, 1601, 1883
SnaBI	TACGTA	1 cut(s)	2316
SseBI	AGGCCT	1 cut(s)	2419
SspI	AATATT	2 cut(s)	727, 2505
SspMI	CTAG	1 cut(s)	1778
SstI	GAGCTC	2 cut(s)	801, 2517
StuI	AGGCCT	1 cut(s)	2419
StyD4I	CCNGG	8 cut(s)	135, 169, 287, 752, 1399, 2020, 2237, 2238
StyI	CCWWGG	2 cut(s)	492, 1559
TaaI	ACNGT	7 cut(s)	51, 554, 840, 1094, 2132, 2391, 2569
TaiI	ACGT	4 cut(s)	181, 356, 393, 2318
TaqI	TCGA	4 cut(s)	325, 696, 1656, 2520
TauI	GCSGC	5 cut(s)	160, 323, 369, 2437, 2450
TfiI	GAWTC	6 cut(s)	29, 150, 1496, 1571, 2154, 2297
Tru1I	TTAA	4 cut(s)	1257, 1311, 2304, 2589
Tru9I	TTAA	4 cut(s)	1257, 1311, 2304, 2589
TscAI	CASTG	5 cut(s)	54, 1354, 2096, 2436, 2560
TseFI	GTSAC	2 cut(s)	986, 2551
TseI	GCWGC	3 cut(s)	110, 380, 2009
Tsp45I	GTSAC	2 cut(s)	986, 2551
TspDTI	ATGAA	8 cut(s)	13, 30, 800, 1046, 1160, 1541, 2193, 2211
TspGWI	ACGGA	3 cut(s)	199, 732, 2483
TspMI	CCCGGG	1 cut(s)	2238
TspRI	CASTG	5 cut(s)	54, 1354, 2096, 2436, 2560
VpaK11BI	GGWCC	2 cut(s)	173, 526
XapI	RAATTY	2 cut(s)	17, 1925
XceI	RCATGY	3 cut(s)	625, 1212, 2552
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1718, 1737
XhoI	CTCGAG	1 cut(s)	324
XmaI	CCCGGG	1 cut(s)	2238
XmiI	GTMKAC	3 cut(s)	696, 2214, 2318
XspI	CTAG	1 cut(s)	1778
ZraI	GACGTC	1 cut(s)	391
Zsp2I	ATGCAT	3 cut(s)	1214, 1434, 1673

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.