AT1G65210

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000523
Physical Location & Seq
Forward (+)
24224812 .. 24225815
1004 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65210.1

Sequence Viewer

Length: 747 bp
ATGTCAAAGCCGATAAGAAACTTAGATGGACCAAGTGGAAGAGCTCCAACTGTTCAACAAATAAGAAACAAACATGGGACTAGAGAGGTGATAGTGGACAACGGGATTATTAGTGTCAGTTTCTCGAGTCCCCAAGGACTTATAACTGGCATCAAATACAAAGGAGTCAATAATGTTCTCCATCCACATCAACGAGCTCGAGAATACTTTGTACCGGAGCCATACAAGAACACAATGAACCCTTTATATCTAAACCACACGGACAAGTTTAGGCAGTACGGATTATGGCAACGATACACAGAGTTATATCCAAACCATGACCTCATCTACACAATTGGAGTTAGTAACTATAGCAAAGACTGGTTCTACTCTCAAGTCACAAGAAAAATCGGTGATTCAACGTACACACCAACTACATGGCAGACTGTGTTTCATCTTCCATATGTAAACATGCGAGGTAGCTACACATTGCAGCTAGCTTTAGCCTCGGCTGCATGGGCTAATCTACAAGTACGTTTCAATAACGAATACACGAGGCCATTTTTCTCAACGGGATATATTGGAAGAGATAATGCTATAGCAAGACATGGAATCCATGGACTCTATCGGCTTTATAGCATCAATGTACCTGGAAGGTTGCTACGCACGGGAACCAACACGATTTATCTTCGACAAGCTAAAGCATCAGGACCACTTGAAGGAGTTATGTATGATTACATTCGTCTCGAAGAGCCTTCTAGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

248

Amino Acids

28.63

Weight (kDa)

9.78

Isoelectric Point (pI)

26.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 24 - 67 2.2e-12 Rhamnogalacturonate lyase family
CBM-like PF14683 63 - 242 4.8e-40 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 143
AfaI GTAC 5 cut(s) 213, 278, 404, 513, 627
AfiI CCNNNNNNNGG 1 cut(s) 698
AgsI TTSAA 4 cut(s) 56, 399, 520, 698
AjnI CCWGG 1 cut(s) 628
AluBI AGCT 6 cut(s) 44, 197, 462, 475, 479, 677
AluI AGCT 6 cut(s) 44, 197, 462, 475, 479, 677
Alw21I GWGCWC 2 cut(s) 46, 199
Alw26I GTCTC 1 cut(s) 728
Ama87I CYCGRG 2 cut(s) 124, 198
AoxI GGCC 1 cut(s) 536
ApeKI GCWGC 2 cut(s) 472, 491
AspS9I GGNCC 2 cut(s) 29, 689
AsuHPI GGTGA 2 cut(s) 100, 404
AsuNHI GCTAGC 1 cut(s) 475
AvaI CYCGRG 2 cut(s) 124, 198
AvaII GGWCC 2 cut(s) 29, 689
BanII GRGCYC 2 cut(s) 46, 199
BauI CACGAG 1 cut(s) 532
Bbv12I GWGCWC 2 cut(s) 46, 199
BbvI GCAGC 2 cut(s) 478, 484
BccI CCATC 2 cut(s) 20, 189
BciT130I CCWGG 1 cut(s) 630
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 3 cut(s) 81, 476, 738
BfmI CTRYAG 2 cut(s) 349, 576
BisI GCNGC 2 cut(s) 473, 492
BlsI GCNGC 2 cut(s) 474, 493
Bme1390I CCNGG 1 cut(s) 630
Bme18I GGWCC 2 cut(s) 29, 689
BmeT110I CYCGRG 2 cut(s) 124, 198
BmgT120I GGNCC 2 cut(s) 29, 689
BmiI GGNNCC 2 cut(s) 219, 652
BmrFI CCNGG 1 cut(s) 630
BmsI GCATC 3 cut(s) 159, 627, 692
BmtI GCTAGC 1 cut(s) 479
BpuEI CTTGAG 1 cut(s) 357
BsaJI CCNNGG 3 cut(s) 133, 486, 595
BsaWI WCCGGW 1 cut(s) 214
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse1I ACTGG 2 cut(s) 151, 365
Bse3DI GCAATG 1 cut(s) 467
BseBI CCWGG 1 cut(s) 630
BseDI CCNNGG 3 cut(s) 133, 486, 595
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMI GCAATG 1 cut(s) 467
BseNI ACTGG 2 cut(s) 151, 365
BseXI GCAGC 2 cut(s) 478, 484
BshFI GGCC 1 cut(s) 538
BsiHKAI GWGCWC 2 cut(s) 46, 199
BsiHKCI CYCGRG 2 cut(s) 124, 198
BsiSI CCGG 1 cut(s) 215
BslFI GGGAC 2 cut(s) 91, 114
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 1 cut(s) 728
BsmBI CGTCTC 1 cut(s) 728
BsmFI GGGAC 2 cut(s) 91, 114
BsnI GGCC 1 cut(s) 538
BsoBI CYCGRG 2 cut(s) 124, 198
Bsp1286I GDGCHC 2 cut(s) 46, 199
Bsp19I CCATGG 1 cut(s) 595
BspANI GGCC 1 cut(s) 538
BspLI GGNNCC 2 cut(s) 219, 652
BspOI GCTAGC 1 cut(s) 479
BspQI GCTCTTC 2 cut(s) 34, 723
BsrDI GCAATG 1 cut(s) 467
BsrI ACTGG 2 cut(s) 151, 365
BssECI CCNNGG 3 cut(s) 133, 486, 595
BssSI CACGAG 1 cut(s) 532
BssT1I CCWWGG 2 cut(s) 133, 595
Bst2BI CACGAG 1 cut(s) 532
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 2 cut(s) 52, 427
Bst6I CTCTTC 3 cut(s) 34, 559, 723
BstC8I GCNNGC 1 cut(s) 477
BstDEI CTNAG 1 cut(s) 22
BstDSI CCRYGG 1 cut(s) 595
BstF5I GGATG 1 cut(s) 181
BstMAI GTCTC 1 cut(s) 728
BstMWI GCNNNNNNNGC 2 cut(s) 491, 497
BstNI CCWGG 1 cut(s) 630
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 628
BstSFI CTRYAG 2 cut(s) 349, 576
BstV1I GCAGC 2 cut(s) 478, 484
BstXI CCANNNNNNTGG 1 cut(s) 417
BsuRI GGCC 1 cut(s) 538
BtgI CCRYGG 1 cut(s) 595
BtsCI GGATG 1 cut(s) 181
Cac8I GCNNGC 1 cut(s) 477
Cfr13I GGNCC 2 cut(s) 29, 689
Csp6I GTAC 5 cut(s) 212, 277, 403, 512, 626
CviAII CATG 7 cut(s) 74, 317, 417, 451, 495, 587, 596
CviQI GTAC 5 cut(s) 212, 277, 403, 512, 626
DdeI CTNAG 1 cut(s) 22
Eam1104I CTCTTC 3 cut(s) 34, 559, 723
EarI CTCTTC 3 cut(s) 34, 559, 723
Ecl136II GAGCTC 2 cut(s) 44, 197
Eco130I CCWWGG 2 cut(s) 133, 595
Eco24I GRGCYC 2 cut(s) 46, 199
Eco47I GGWCC 2 cut(s) 29, 689
Eco53kI GAGCTC 2 cut(s) 44, 197
Eco88I CYCGRG 2 cut(s) 124, 198
EcoICRI GAGCTC 2 cut(s) 44, 197
EcoRII CCWGG 1 cut(s) 628
EcoT14I CCWWGG 2 cut(s) 133, 595
EcoT38I GRGCYC 2 cut(s) 46, 199
ErhI CCWWGG 2 cut(s) 133, 595
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 7 cut(s) 77, 320, 420, 454, 498, 590, 599
FaqI GGGAC 2 cut(s) 91, 114
FatI CATG 7 cut(s) 73, 316, 416, 450, 494, 586, 595
FauNDI CATATG 1 cut(s) 442
Fnu4HI GCNGC 2 cut(s) 473, 492
FokI GGATG 1 cut(s) 168
FriOI GRGCYC 2 cut(s) 46, 199
Fsp4HI GCNGC 2 cut(s) 473, 492
FspBI CTAG 3 cut(s) 81, 476, 738
GluI GCNGC 2 cut(s) 473, 492
HaeIII GGCC 1 cut(s) 538
HapII CCGG 1 cut(s) 215
Hin1II CATG 7 cut(s) 77, 320, 420, 454, 498, 590, 599
HinfI GANTC 5 cut(s) 127, 165, 395, 591, 600
HpaII CCGG 1 cut(s) 215
HphI GGTGA 2 cut(s) 100, 404
Hpy166II GTNNAC 3 cut(s) 97, 405, 448
Hpy188III TCNNGA 5 cut(s) 124, 200, 687, 725, 738
Hpy8I GTNNAC 3 cut(s) 97, 405, 448
HpyAV CCTTC 3 cut(s) 627, 692, 744
HpyCH4III ACNGT 2 cut(s) 52, 427
HpyCH4IV ACGT 2 cut(s) 401, 514
HpyCH4V TGCA 2 cut(s) 472, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 497
HpyF3I CTNAG 1 cut(s) 22
HpySE526I ACGT 2 cut(s) 401, 514
Hsp92II CATG 7 cut(s) 77, 320, 420, 454, 498, 590, 599
LguI GCTCTTC 2 cut(s) 34, 723
LmnI GCTCC 2 cut(s) 49, 217
LpnPI CCDG 6 cut(s) 132, 228, 346, 615, 642, 672
Lsp1109I GCAGC 2 cut(s) 478, 484
LweI GCATC 3 cut(s) 159, 627, 692
MaeI CTAG 3 cut(s) 81, 476, 738
MaeII ACGT 2 cut(s) 401, 514
MaeIII GTNAC 2 cut(s) 344, 376
MboII GAAGA 5 cut(s) 51, 428, 576, 659, 740
MfeI CAATTG 1 cut(s) 333
MhlI GDGCHC 2 cut(s) 46, 199
MluCI AATT 1 cut(s) 333
MlyI GAGTC 3 cut(s) 136, 174, 594
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 5 cut(s) 79, 332, 449, 496, 528
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 1 cut(s) 630
MunI CAATTG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 630
MwoI GCNNNNNNNGC 2 cut(s) 491, 497
NcoI CCATGG 1 cut(s) 595
NdeI CATATG 1 cut(s) 442
NheI GCTAGC 1 cut(s) 475
NlaIII CATG 7 cut(s) 77, 320, 420, 454, 498, 590, 599
NlaIV GGNNCC 2 cut(s) 219, 652
NmeAIII GCCGAG 1 cut(s) 467
NmuCI GTSAC 1 cut(s) 376
NspI RCATGY 1 cut(s) 454
PaeR7I CTCGAG 2 cut(s) 124, 198
PciSI GCTCTTC 2 cut(s) 34, 723
PfeI GAWTC 2 cut(s) 395, 591
PkrI GCNGC 2 cut(s) 474, 493
PleI GAGTC 3 cut(s) 135, 173, 594
PpsI GAGTC 3 cut(s) 135, 173, 594
PsiI TTATAA 1 cut(s) 143
Psp124BI GAGCTC 2 cut(s) 46, 199
Psp6I CCWGG 1 cut(s) 628
PspGI CCWGG 1 cut(s) 628
PspN4I GGNNCC 2 cut(s) 219, 652
PspPI GGNCC 2 cut(s) 29, 689
RsaI GTAC 5 cut(s) 213, 278, 404, 513, 627
RsaNI GTAC 5 cut(s) 212, 277, 403, 512, 626
SacI GAGCTC 2 cut(s) 46, 199
SapI GCTCTTC 2 cut(s) 34, 723
SatI GCNGC 2 cut(s) 473, 492
Sau96I GGNCC 2 cut(s) 29, 689
SchI GAGTC 3 cut(s) 136, 174, 594
ScrFI CCNGG 1 cut(s) 630
SduI GDGCHC 2 cut(s) 46, 199
SfaNI GCATC 3 cut(s) 159, 627, 692
SfcI CTRYAG 2 cut(s) 349, 576
Sfr274I CTCGAG 2 cut(s) 124, 198
SinI GGWCC 2 cut(s) 29, 689
SlaI CTCGAG 2 cut(s) 124, 198
SmlI CTYRAG 3 cut(s) 124, 198, 372
SmoI CTYRAG 3 cut(s) 124, 198, 372
Sse9I AATT 1 cut(s) 333
SspMI CTAG 3 cut(s) 81, 476, 738
SstI GAGCTC 2 cut(s) 46, 199
StyD4I CCNGG 1 cut(s) 628
StyI CCWWGG 2 cut(s) 133, 595
TaaI ACNGT 2 cut(s) 52, 427
TaiI ACGT 2 cut(s) 404, 517
TaqI TCGA 4 cut(s) 125, 199, 670, 726
TasI AATT 1 cut(s) 333
TfiI GAWTC 2 cut(s) 395, 591
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 2 cut(s) 472, 491
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 2 cut(s) 251, 422
TspGWI ACGGA 2 cut(s) 275, 294
VpaK11BI GGWCC 2 cut(s) 29, 689
XbaI TCTAGA 1 cut(s) 737
XceI RCATGY 1 cut(s) 454
XhoI CTCGAG 2 cut(s) 124, 198
XspI CTAG 3 cut(s) 81, 476, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.