Rorug02G0166900

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000523
Physical Location & Seq
Forward (+)
14972618 .. 14976832
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0166900.1

Sequence Viewer

Length: 2487 bp
ATGATCAATCCACTGGATAAACTTTTCAGTGATCTCTGCGATGGCTTTAAAGACCTCATCAAGAAGAATATTCAATTTAAAGGCAAAGTCGACGGCATCGAAGACAAGCTAAAAGCTCTAGAGCCGCTGATCCGAAACATGGTGCGGAATAATGAGGTATTAGATCGCCCAAGGAACGAACTAAAAGATTTAACAGAAGTTATGAAGTCTGGCATCAAGCTCACTGTCAAGTGCTCAAAGTCTCGGAGATGGGAGTTCTACTGGAGGTACAAATACGCGAACAAACTTACTGAATGGGAGAAACGTCTACAAAGTCTGTTAGAGAGACTGATTGTGCACACCGCCAGGGATGTGAAGGAGATTGCAGGGGATGTGAAGGAGATTGGAGGGGATGTGAAGGAGATTGCAGGGGATGTGAAGGAGATTGGAGGGTTTGTGAAGGAGATTGCAGGGGATGTGAAGGAGATTGGAGGGTTTGTGAAGGAGATTGCCGGGGTTGTGTTGGGCCGAATTGTGATACAACCTGGGGAATCCAAAGCTTGGATGACTACAGTAGTACCTGAAGTTCCACGTTTTACAGTGGGATTGGACACGCATTTGAAGGAATTGAAGATGAAGCTTCTCAAGAATGGGGGGTCAATGCTTGTAATAACAGCTCCTGGAGGGTCTGGCAAAACCACTTTGGCTACAATGTTTTATCATGATCATGAAGTCACAGATAAATTCCCCAAGAACATCTTTGTTACTGTTTCAACAAAGTCCAACTGCATGGTTGTACAAGAACTATGTCAAAAGTTGGGATCCCAGGAATCTGCATTCCCAAATGAAGACATTGCATTCAGCAAGCTGAAGGAATTTCTGAAGGAAACCTCACAAAAGCCTTTGCTTCTCATCCTGGATGATGTTTTGTCTGAATCAGAATCCCTTCTTGAGAAGTTTAATGAACCAAGTATACAATCAAATCTCAGTATCTTGGTGACATCAAGATCTTCATTTCCAAGTGTCGGTTCTCAATATCCTTTAGATGCATTGAATCACAAAGATGCAATGACCCTTTTTCGCCATTCAGCAATCCGGGGAGATAAGAGCAATAATGTTACAGAAAATCTTCAGGACAAGATAGTAAGGCGCTGTAAGGGATGTCCACTTGCCATTAAAGTGGTCGGAAAATCTCTTTGTGGAAAATTTACAGAGTCATGGCTTAAAAAATTAAAGGAGTGGTCTGAAGGTGCTTCTATTTTTGATACCGAGACTGAAGAGCTTCTTTGCCTCCAAAGTAGCTTAGATCAAACTATCAAGGAATGCTTCTTGGACCTGGGTGCATTTCCTGAAGATCAAAGAATCCCAGCTGTTGCTCTGATTGATATGTGGGCGGAGTTATATGGCATGGAAGATTTTCTTTCCATTCAAAAGCTCCAGGAGCTTTCCAGTCGAAGCCTGGCTAGTCTTGTAGTCACAAGGAAGGAAAAGATGGAGGCGGATGGCTACTACAGTGAACACTTTGTCTCCCAGCATGATCTGCTCAGGGATCTAGCTATCTACCAAGCGAAATTAGATCCAAACAAAACGAGACTGATTTTACACAAATGTGGAGACAAAGTTCCCAAGAAGTTGATAGAACAGAAGCATCAATCCTTTCAGACTCGCGTATTATCCATCTCCTCTGATGGAGTGTTCTCAACAGATATGCACAAGATTCAACTTTCAGATGTTGAGGTTCTAGTTTTGAATTTTCAGTCAGAGAACTATGCTTTACCCAACTTTGTGGCGGACATGGAAAAGTTGAAGGTTCTAATAGTCACAAATAATGGTTCCTTACCAGCTGAATTGAGTAACTTTGAACTACTGAATTCCTTGCCAAATCTGAAGAGAATCAGACTAGAGCGAATTTCAATTCCTTCCATAACCAAGAATCCCATACGGTTGAAGAGTGTAAAGAAGATCTCTTTATTCATGTGTAGCATCGGTGAAGCTTTCAGCAACTCTTCCTTCCAAATTTCATATGCATTTCCAAATCTACAGGAATTGAACATTGACTATTGCAGTGACTTGGTGGAATTATCTGGTAATCTCAGTGATCTGATTGAGCTAAAGAATCTCAGTATCACCCACTGTCACAAGCTATCTGCCTTGCCTGAAAAGATCGGTAATTTGATCAAATTGGAAGTCTTGAGGCTAAAGTCATGTTCCGACTTGGTAACATTTCCAGCCTCAATCAAGAACCTCGGAAAGTTGCAGATTCTCGACATATCTGATTGCTTCAGCATCAAGGAGTTGCCTGAAGACATTGGTGAAATCAGCACTTTAAAAAAGATCAATATGAGACAGTGCTCTAGACTGAGGGATCTACCTGAATCAGTTTCTGATCTCGAGCAACTAGAGGAAGTGATATGTGATGAGGAAACAAAAAAGTGGTGGGAGCTTTTCCGGCTCAAAACAGAGATTAGGGTCCTCACAGACGAATTCAACCTGGATTGGCTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

828

Amino Acids

93.81

Weight (kDa)

6.22

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 132 1e-15 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 197 - 356 5.5e-17 NB-ARC domain
AAA_22 PF13401 211 - 311 5.6e-06 AAA domain
LRR_14 PF23598 547 - 714 2.2e-06 Leucine-rich repeat region
LRR_14 PF23598 714 - 820 1.3e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 540
AccI GTMKAC 3 cut(s) 90, 307, 952
AccII CGCG 2 cut(s) 278, 1647
AciI CCGC 6 cut(s) 125, 145, 342, 1373, 1478, 1769
AclWI GGATC 6 cut(s) 124, 795, 808, 1536, 1550, 2352
AcsI RAATTY 8 cut(s) 722, 854, 1184, 1729, 1849, 1887, 1995, 2462
AfaI GTAC 3 cut(s) 269, 558, 777
AfiI CCNNNNNNNGG 3 cut(s) 139, 540, 1004
AjnI CCWGG 9 cut(s) 344, 523, 658, 804, 894, 1314, 1416, 1437, 2469
AjuI GAANNNNNNNTTGG 2 cut(s) 991, 1023
AleI CACNNNNGTG 1 cut(s) 1587
AloI GAACNNNNNNTCC 2 cut(s) 1584, 1616
Alw21I GWGCWC 3 cut(s) 236, 339, 2333
Alw26I GTCTC 7 cut(s) 246, 319, 1244, 1510, 1564, 1587, 2317
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 6 cut(s) 124, 795, 808, 1536, 1550, 2352
AlwNI CAGNNNCTG 2 cut(s) 659, 2363
Ama87I CYCGRG 1 cut(s) 2369
AoxI GGCC 1 cut(s) 505
ApaLI GTGCAC 1 cut(s) 335
ApoI RAATTY 8 cut(s) 722, 854, 1184, 1729, 1849, 1887, 1995, 2462
ArsI GACNNNNNNTTYG 2 cut(s) 2151, 2183
Asp700I GAANNNNTTC 4 cut(s) 924, 1107, 1260, 1395
AspLEI GCGC 1 cut(s) 1131
AspS9I GGNCC 3 cut(s) 505, 1312, 2449
AsuC2I CCSGG 2 cut(s) 493, 1076
AsuHPI GGTGA 4 cut(s) 988, 1979, 2098, 2303
AvaI CYCGRG 1 cut(s) 2369
AvaII GGWCC 2 cut(s) 1312, 2449
BaeGI GKGCMC 1 cut(s) 339
BaeI ACNNNNGTAYC 2 cut(s) 540, 573
BamHI GGATCC 1 cut(s) 800
BbsI GAAGAC 3 cut(s) 108, 834, 2289
Bbv12I GWGCWC 3 cut(s) 236, 339, 2333
BccI CCATC 6 cut(s) 35, 243, 1465, 1475, 1661, 1664
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 9 cut(s) 346, 525, 660, 806, 896, 1316, 1418, 1439, 2471
BclI TGATCA 3 cut(s) 3, 703, 2154
BcnI CCSGG 2 cut(s) 493, 1076
BcoDI GTCTC 7 cut(s) 246, 319, 1244, 1510, 1564, 1587, 2317
BfaI CTAG 7 cut(s) 119, 1443, 1532, 1721, 1880, 2334, 2378
BfmI CTRYAG 3 cut(s) 549, 1489, 2018
BfoI RGCGCY 1 cut(s) 1132
BglII AGATCT 2 cut(s) 986, 1941
BisI GCNGC 1 cut(s) 125
BlsI GCNGC 1 cut(s) 126
Bme18I GGWCC 2 cut(s) 1312, 2449
BmeT110I CYCGRG 1 cut(s) 2369
BmgT120I GGNCC 3 cut(s) 505, 1312, 2449
BmiI GGNNCC 3 cut(s) 802, 1813, 2450
BmsI GCATC 7 cut(s) 105, 222, 1015, 1033, 1636, 1971, 2274
BpiI GAAGAC 3 cut(s) 108, 834, 2289
BpmI CTGGAG 3 cut(s) 283, 681, 1400
Bpu10I CCTNAGC 1 cut(s) 1523
BpuEI CTTGAG 3 cut(s) 608, 950, 2191
BpuMI CCSGG 2 cut(s) 493, 1076
BsaJI CCNNGG 8 cut(s) 170, 345, 492, 524, 804, 1075, 1315, 2224
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 540, 1004
Bse1I ACTGG 3 cut(s) 18, 266, 1428
Bse3DI GCAATG 2 cut(s) 831, 1053
BseBI CCWGG 9 cut(s) 346, 525, 660, 806, 896, 1316, 1418, 1439, 2471
BseDI CCNNGG 8 cut(s) 170, 345, 492, 524, 804, 1075, 1315, 2224
BseLI CCNNNNNNNGG 3 cut(s) 139, 540, 1004
BseMI GCAATG 2 cut(s) 831, 1053
BseMII CTCAG 5 cut(s) 979, 1537, 2086, 2113, 2330
BseNI ACTGG 3 cut(s) 18, 266, 1428
BseRI GAGGAG 1 cut(s) 1651
BseSI GKGCMC 1 cut(s) 339
BseYI CCCAGC 2 cut(s) 1345, 1509
Bsh1236I CGCG 2 cut(s) 278, 1647
BshFI GGCC 1 cut(s) 507
BsiHKAI GWGCWC 3 cut(s) 236, 339, 2333
BsiHKCI CYCGRG 1 cut(s) 2369
BsiSI CCGG 3 cut(s) 492, 1075, 2428
BslI CCNNNNNNNGG 3 cut(s) 139, 540, 1004
BsmAI GTCTC 7 cut(s) 246, 319, 1244, 1510, 1564, 1587, 2317
BsmI GAATGC 3 cut(s) 815, 836, 1307
BsnI GGCC 1 cut(s) 507
BsoBI CYCGRG 1 cut(s) 2369
Bsp1286I GDGCHC 3 cut(s) 236, 339, 2333
Bsp1407I TGTACA 1 cut(s) 775
BspACI CCGC 6 cut(s) 125, 145, 342, 1373, 1478, 1769
BspANI GGCC 1 cut(s) 507
BspCNI CTCAG 5 cut(s) 978, 1536, 2085, 2112, 2331
BspFNI CGCG 2 cut(s) 278, 1647
BspHI TCATGA 2 cut(s) 700, 706
BspLI GGNNCC 3 cut(s) 802, 1813, 2450
BspPI GGATC 6 cut(s) 124, 795, 808, 1536, 1550, 2352
BspQI GCTCTTC 1 cut(s) 1251
BsrDI GCAATG 2 cut(s) 831, 1053
BsrGI TGTACA 1 cut(s) 775
BsrI ACTGG 3 cut(s) 18, 266, 1428
BssECI CCNNGG 8 cut(s) 170, 345, 492, 524, 804, 1075, 1315, 2224
BssNAI GTATAC 1 cut(s) 953
BssT1I CCWWGG 1 cut(s) 170
Bst1107I GTATAC 1 cut(s) 953
Bst2UI CCWGG 9 cut(s) 346, 525, 660, 806, 896, 1316, 1418, 1439, 2471
Bst4CI ACNGT 8 cut(s) 226, 553, 580, 748, 1493, 1923, 2114, 2328
Bst6I CTCTTC 4 cut(s) 1251, 1862, 1922, 1990
BstAPI GCANNNNNTGC 1 cut(s) 1519
BstAUI TGTACA 1 cut(s) 775
BstC8I GCNNGC 1 cut(s) 845
BstDEI CTNAG 6 cut(s) 965, 1282, 1523, 2072, 2099, 2339
BstFNI CGCG 2 cut(s) 278, 1647
BstH2I RGCGCY 1 cut(s) 1132
BstHHI GCGC 1 cut(s) 1131
BstMAI GTCTC 7 cut(s) 246, 319, 1244, 1510, 1564, 1587, 2317
BstMWI GCNNNNNNNGC 3 cut(s) 1420, 1519, 2428
BstNI CCWGG 9 cut(s) 346, 525, 660, 806, 896, 1316, 1418, 1439, 2471
BstSFI CTRYAG 3 cut(s) 549, 1489, 2018
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 2 cut(s) 278, 1647
BstV2I GAAGAC 3 cut(s) 108, 834, 2289
BstX2I RGATCY 6 cut(s) 800, 986, 1528, 1555, 1941, 2344
BstXI CCANNNNNNTGG 3 cut(s) 769, 1159, 1765
BstYI RGATCY 6 cut(s) 800, 986, 1528, 1555, 1941, 2344
BstZ17I GTATAC 1 cut(s) 953
BsuRI GGCC 1 cut(s) 507
BtgZI GCGATG 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 2050
BtsIMutI CAGTG 9 cut(s) 11, 34, 222, 585, 1498, 2050, 2080, 2110, 2333
Cac8I GCNNGC 1 cut(s) 845
CaiI CAGNNNCTG 2 cut(s) 659, 2363
CciI TCATGA 2 cut(s) 700, 706
CfoI GCGC 1 cut(s) 1131
Cfr13I GGNCC 3 cut(s) 505, 1312, 2449
Csp6I GTAC 3 cut(s) 268, 557, 776
CviQI GTAC 3 cut(s) 268, 557, 776
DdeI CTNAG 6 cut(s) 965, 1282, 1523, 2072, 2099, 2339
DraI TTTAAA 3 cut(s) 49, 79, 2307
Eam1104I CTCTTC 4 cut(s) 1251, 1862, 1922, 1990
EarI CTCTTC 4 cut(s) 1251, 1862, 1922, 1990
EciI GGCGGA 3 cut(s) 1388, 1493, 1784
Eco130I CCWWGG 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 1312, 2449
Eco88I CYCGRG 1 cut(s) 2369
EcoO109I RGGNCCY 1 cut(s) 2449
EcoRI GAATTC 2 cut(s) 1849, 2462
EcoRII CCWGG 9 cut(s) 344, 523, 658, 804, 894, 1314, 1416, 1437, 2469
EcoT14I CCWWGG 1 cut(s) 170
EcoT22I ATGCAT 2 cut(s) 1030, 2008
ErhI CCWWGG 1 cut(s) 170
FalI AAGNNNNNCTT 8 cut(s) 722, 754, 1248, 1280, 1289, 1321, 1383, 1415
FauNDI CATATG 1 cut(s) 2002
FbaI TGATCA 3 cut(s) 3, 703, 2154
FblI GTMKAC 3 cut(s) 90, 307, 952
Fnu4HI GCNGC 1 cut(s) 125
Fsp4HI GCNGC 1 cut(s) 125
FspBI CTAG 7 cut(s) 119, 1443, 1532, 1721, 1880, 2334, 2378
GlaI GCGC 1 cut(s) 1130
GluI GCNGC 1 cut(s) 125
GsaI CCCAGC 2 cut(s) 1349, 1513
GsuI CTGGAG 3 cut(s) 283, 681, 1400
HaeII RGCGCY 1 cut(s) 1132
HaeIII GGCC 1 cut(s) 507
HapII CCGG 3 cut(s) 492, 1075, 2428
HhaI GCGC 1 cut(s) 1131
Hin6I GCGC 1 cut(s) 1129
HinP1I GCGC 1 cut(s) 1129
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 3 cut(s) 537, 617, 1971
HpaII CCGG 3 cut(s) 492, 1075, 2428
HphI GGTGA 4 cut(s) 988, 1979, 2098, 2303
Hpy166II GTNNAC 6 cut(s) 91, 308, 337, 953, 1145, 1496
Hpy8I GTNNAC 6 cut(s) 91, 308, 337, 953, 1145, 1496
Hpy99I CGWCG 1 cut(s) 95
HpyCH4III ACNGT 8 cut(s) 226, 553, 580, 748, 1493, 1923, 2114, 2328
HpyCH4IV ACGT 2 cut(s) 304, 571
HpyF10VI GCNNNNNNNGC 3 cut(s) 1420, 1519, 2428
HpyF3I CTNAG 6 cut(s) 965, 1282, 1523, 2072, 2099, 2339
HpySE526I ACGT 2 cut(s) 304, 571
HspAI GCGC 1 cut(s) 1129
Ksp22I TGATCA 3 cut(s) 3, 703, 2154
LguI GCTCTTC 1 cut(s) 1251
LmnI GCTCC 4 cut(s) 661, 1419, 1420, 2419
LweI GCATC 7 cut(s) 105, 222, 1015, 1033, 1636, 1971, 2274
MaeI CTAG 7 cut(s) 119, 1443, 1532, 1721, 1880, 2334, 2378
MaeII ACGT 2 cut(s) 304, 571
MflI RGATCY 6 cut(s) 800, 986, 1528, 1555, 1941, 2344
MhlI GDGCHC 3 cut(s) 236, 339, 2333
MlyI GAGTC 2 cut(s) 1202, 1636
MmeI TCCRAC 3 cut(s) 786, 1144, 2214
Mph1103I ATGCAT 2 cut(s) 1030, 2008
MroXI GAANNNNTTC 4 cut(s) 924, 1107, 1260, 1395
MseI TTAA 8 cut(s) 48, 78, 191, 939, 1155, 1203, 1211, 2306
MslI CAYNNNNRTG 4 cut(s) 705, 1041, 1157, 1587
MspA1I CMGCKG 3 cut(s) 127, 1349, 1823
MspI CCGG 3 cut(s) 492, 1075, 2428
Mva1269I GAATGC 3 cut(s) 815, 836, 1307
MvaI CCWGG 9 cut(s) 346, 525, 660, 806, 896, 1316, 1418, 1439, 2471
MvnI CGCG 2 cut(s) 278, 1647
MwoI GCNNNNNNNGC 3 cut(s) 1420, 1519, 2428
NciI CCSGG 2 cut(s) 493, 1076
NdeI CATATG 1 cut(s) 2002
NlaIV GGNNCC 3 cut(s) 802, 1813, 2450
NmuCI GTSAC 6 cut(s) 712, 976, 1453, 1798, 2045, 2114
NsiI ATGCAT 2 cut(s) 1030, 2008
OliI CACNNNNGTG 1 cut(s) 1587
PaeR7I CTCGAG 1 cut(s) 2369
PagI TCATGA 2 cut(s) 700, 706
PciSI GCTCTTC 1 cut(s) 1251
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 3 cut(s) 815, 836, 1307
PdmI GAANNNNTTC 4 cut(s) 924, 1107, 1260, 1395
PflMI CCANNNNNTGG 1 cut(s) 540
PfoI TCCNGGA 3 cut(s) 658, 894, 1416
PkrI GCNGC 1 cut(s) 126
PleI GAGTC 2 cut(s) 1201, 1636
PpsI GAGTC 2 cut(s) 1201, 1636
PpuMI RGGWCCY 1 cut(s) 2449
Psp5II RGGWCCY 1 cut(s) 2449
Psp6I CCWGG 9 cut(s) 344, 523, 658, 804, 894, 1314, 1416, 1437, 2469
PspFI CCCAGC 2 cut(s) 1345, 1509
PspGI CCWGG 9 cut(s) 344, 523, 658, 804, 894, 1314, 1416, 1437, 2469
PspN4I GGNNCC 3 cut(s) 802, 1813, 2450
PspPI GGNCC 3 cut(s) 505, 1312, 2449
PspPPI RGGWCCY 1 cut(s) 2449
PsrI GAACNNNNNNTAC 4 cut(s) 549, 581, 936, 968
PstNI CAGNNNCTG 2 cut(s) 659, 2363
PsuI RGATCY 6 cut(s) 800, 986, 1528, 1555, 1941, 2344
PvuII CAGCTG 2 cut(s) 1349, 1823
RsaI GTAC 3 cut(s) 269, 558, 777
RsaNI GTAC 3 cut(s) 268, 557, 776
RseI CAYNNNNRTG 4 cut(s) 705, 1041, 1157, 1587
SalI GTCGAC 1 cut(s) 89
SapI GCTCTTC 1 cut(s) 1251
SaqAI TTAA 8 cut(s) 48, 78, 191, 939, 1155, 1203, 1211, 2306
SatI GCNGC 1 cut(s) 125
Sau96I GGNCC 3 cut(s) 505, 1312, 2449
SchI GAGTC 2 cut(s) 1202, 1636
SduI GDGCHC 3 cut(s) 236, 339, 2333
SfaNI GCATC 7 cut(s) 105, 222, 1015, 1033, 1636, 1971, 2274
SfcI CTRYAG 3 cut(s) 549, 1489, 2018
Sfr274I CTCGAG 1 cut(s) 2369
SinI GGWCC 2 cut(s) 1312, 2449
SlaI CTCGAG 1 cut(s) 2369
SmiMI CAYNNNNRTG 4 cut(s) 705, 1041, 1157, 1587
SmlI CTYRAG 4 cut(s) 623, 929, 2170, 2369
SmoI CTYRAG 4 cut(s) 623, 929, 2170, 2369
SsiI CCGC 6 cut(s) 125, 145, 342, 1373, 1478, 1769
SspI AATATT 1 cut(s) 70
SspMI CTAG 7 cut(s) 119, 1443, 1532, 1721, 1880, 2334, 2378
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 8 cut(s) 226, 553, 580, 748, 1493, 1923, 2114, 2328
TaiI ACGT 2 cut(s) 307, 574
TaqI TCGA 5 cut(s) 90, 99, 1432, 2244, 2370
TatI WGTACW 1 cut(s) 775
TauI GCSGC 1 cut(s) 127
Tru1I TTAA 8 cut(s) 48, 78, 191, 939, 1155, 1203, 1211, 2306
Tru9I TTAA 8 cut(s) 48, 78, 191, 939, 1155, 1203, 1211, 2306
TscAI CASTG 9 cut(s) 18, 34, 229, 585, 1498, 2050, 2080, 2117, 2333
TseFI GTSAC 6 cut(s) 712, 976, 1453, 1798, 2045, 2114
Tsp45I GTSAC 6 cut(s) 712, 976, 1453, 1798, 2045, 2114
TspDTI ATGAA 8 cut(s) 218, 629, 723, 840, 957, 981, 1942, 1989
TspRI CASTG 9 cut(s) 18, 34, 229, 585, 1498, 2050, 2080, 2117, 2333
Van91I CCANNNNNTGG 1 cut(s) 540
VneI GTGCAC 1 cut(s) 335
VpaK11BI GGWCC 2 cut(s) 1312, 2449
XapI RAATTY 8 cut(s) 722, 854, 1184, 1729, 1849, 1887, 1995, 2462
XbaI TCTAGA 2 cut(s) 118, 2333
XcmI CCANNNNNNNNNTGG 1 cut(s) 1435
XhoI CTCGAG 1 cut(s) 2369
XmiI GTMKAC 3 cut(s) 90, 307, 952
XmnI GAANNNNTTC 4 cut(s) 924, 1107, 1260, 1395
XspI CTAG 7 cut(s) 119, 1443, 1532, 1721, 1880, 2334, 2378
Zsp2I ATGCAT 2 cut(s) 1030, 2008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.