MD04G1240500.v1.1

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000523
Physical Location & Seq
Reverse (-)
31810363 .. 31812402
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1240500.v1.1.491

Sequence Viewer

Length: 2040 bp
ATGGAGGAGGTGAGGCAATGGAGGAGATGGGGTATCCTCCGAGTTTTCTCAGTCGCACTATGTTTGCTCTTCTTCCTCCACAATAATAATGGTGCTTTTGAGACGATAACGATTTCTGGAAGGCGAGCACTAAGGAGTGTTAATGGTAATGAGCTCAATTTCAACCAGATGCTAACACAACAAACTCATAACACGGTGGAGCTGAACAATGGCATTGTCCGAGTCACTTTTTCTTATCCTGGAGGAGATGTCATTGGAATTCAATACAAGGGAATTGACAACTTGCTCGAAATCAAAAACCCCCCAAGCAATAGAGGTTACTGGGATCTTGTCTCGAAAAATGGCTTGGACAGACTACAAGGAACAACATTCAAGATTGTTACATCGACGGCAGACCAAATAGAGATTTCTTTTAATAAAACTTTTGATATTTCCCTCGGTGATTGGACGCTACCCTTAAATGTTGACAAAAGATACATAATGCAGCGTGGCCGCGCTGGGTTCTATGCATATGCCATATATGAGCGTCTAGAAGGGTGGCCGGAATTGCACCTAGGTCAAACAAGACTCGTTTTGAAACTTCGAGAAGATAAATTTCAATTCATGGCTGTATCAGACGACAGACGAAGATTCATGCCAACAGCCAAAGATCGCGACGGAGACCACGCTCAGGCCCTTGCCTATCGCGAAGCTGTTCTTTTAACTAATCCAAGCAATCCCGACTTTAAAGGAGAGGTGGATGACAAATACCAATACTCCAGTGCAGTCAAAGACATCAAGGTTCACGGTTGGATTAGCACTGACGATGCTATAGGTCTTTGGATGATCACACCTAGTACTGAGTTTCGTTCAGCGGGGCCGTTCAAGCAGGAACTTACCTCTCATGTGGGACCAACTTCCCTCACTGTGTTTCTTAGTAGTCACTACGTCGGGAGAGGTTTTGGACTGACCTTCGCAGAAGGTGAGGCATGGAAGAAGGTTTTTGGTCCTGTCTTTGTGTATCTTAACTCGGACATTCCAAGCTCGAATGCCTCCTTCTCTCTCTGGGAAAATGCAAAGGAACAGATGTATGAAGAAGTAAAGAGTTGGCCGTACAATTTCACTCAGTCCGAAGACTTTCCTAGCTCCGATGAACGTGGATCAGTTGCTGGTCAGTTACTAATATGGGATTCGTACTTCCACGAGGGCCTAGATTCAGCAAGTTATGCTTATGTGGGATTGGCTGCACGCGGATACGCGGGATCATGGCAAATGGAATGCAAGGGTTATCAGTTCTGGACTCAAGCTGACAAGCAAGGTTATTTCTACATAAAAGATGTTCGACCGGGGAACTATAGTTTGTATGCAACTGTTCCCGGCTTCATTGGGGACTACAAGTACGAGCATGATATTACAATTGAACCAGCAAGTGAAATCAACTTAGCTAATATTACCTTCGAACCTCCGAGAAGTGGTCCAACCTTGTGGGAGATTGGCATCCCCGATCGGTCAGCCGCAGAGTTCAACATACCGGATCCATATCCGACCCTTGTGAACCGTTTGTTCACTGACAATTACGCAAACAAGTTTAGGCAATATGGCTTGTGGGAACGATACGGAGATTACTACCCTGACCATGATCTCATCTACACCGTTGGCACAGACAATTACAATGATGATTGGTTTTACGCTCATGTGACAAGAAACAGAAACGGTACATACGAGGCAACGACATGGCAAATTGTATTTCAACTTGAAGACGTTTGGACCGGAGATTATACGCTCCAGTTGGCATTGGCTTCGGCCACCTATGCTGAACTGCAGGTTCGATTCAACAACCAGAACGACACACCTCATTTTTCGACAGGGCTAATAGGGAAGGACAATGCCATAGCAAGGCATGGAATTCATGGTTTGTACTGGCTTTTCAGTGTTGATGTGCCGAGCTATCGACTACAACAAGGATACAACACCATATACCTTACTCAATCAAGAAACTGGGGACCTTTCGCCGGCGTTATGTACGACTACATCCGACTGGAAGGACCTCCACCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

680

Amino Acids

77.91

Weight (kDa)

5.28

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 59 - 246 2.8e-76 Rhamnogalacturonate lyase family
fn3_3 PF14686 402 - 473 1.3e-21 Polysaccharide lyase family 4, domain II
CBM-like PF14683 487 - 673 7.1e-48 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1792
AccII CGCG 5 cut(s) 495, 654, 687, 1230, 1238
AciI CCGC 5 cut(s) 493, 854, 1230, 1238, 1494
AclWI GGATC 5 cut(s) 333, 1147, 1249, 1508, 1521
AcoI YGGCCR 4 cut(s) 490, 539, 1088, 1782
AcsI RAATTY 3 cut(s) 258, 593, 1884
AfaI GTAC 7 cut(s) 838, 1094, 1175, 1379, 1696, 1898, 2003
AfiI CCNNNNNNNGG 4 cut(s) 670, 1451, 1875, 1991
AjnI CCWGG 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AleI CACNNNNGTG 1 cut(s) 2035
AluBI AGCT 8 cut(s) 154, 202, 692, 1023, 1125, 1286, 1424, 1926
AluI AGCT 8 cut(s) 154, 202, 692, 1023, 1125, 1286, 1424, 1926
Alw21I GWGCWC 2 cut(s) 130, 156
Alw26I GTCTC 3 cut(s) 95, 337, 654
AlwI GGATC 5 cut(s) 333, 1147, 1249, 1508, 1521
AlwNI CAGNNNCTG 1 cut(s) 1148
AoxI GGCC 7 cut(s) 490, 539, 672, 857, 1088, 1186, 1782
ApeKI GCWGC 2 cut(s) 484, 1223
ApoI RAATTY 3 cut(s) 258, 593, 1884
Asp700I GAANNNNTTC 2 cut(s) 693, 1116
AspA2I CCTAGG 1 cut(s) 553
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 9 cut(s) 673, 857, 890, 986, 1186, 1454, 1746, 1982, 2024
AsuC2I CCSGG 2 cut(s) 1326, 1356
AsuHPI GGTGA 3 cut(s) 22, 452, 974
AsuII TTCGAA 1 cut(s) 1437
AvaII GGWCC 6 cut(s) 890, 986, 1454, 1746, 1982, 2024
AvrII CCTAGG 1 cut(s) 553
BamHI GGATCC 1 cut(s) 1513
BanII GRGCYC 1 cut(s) 156
BauI CACGAG 1 cut(s) 1181
BbsI GAAGAC 2 cut(s) 1119, 1743
Bbv12I GWGCWC 2 cut(s) 130, 156
BbvI GCAGC 2 cut(s) 496, 1210
BccI CCATC 1 cut(s) 21
BceAI ACGGC 3 cut(s) 405, 844, 1075
BcgI CGANNNNNNTGC 2 cut(s) 1761, 1795
BciT130I CCWGG 1 cut(s) 240
BciVI GTATCC 3 cut(s) 44, 1226, 1937
BclI TGATCA 1 cut(s) 825
BcnI CCSGG 2 cut(s) 1326, 1356
BcoDI GTCTC 3 cut(s) 95, 337, 654
BfaI CTAG 5 cut(s) 530, 554, 834, 1122, 1190
BfmI CTRYAG 3 cut(s) 810, 1333, 1799
BfuAI ACCTGC 1 cut(s) 1792
BfuI GTATCC 3 cut(s) 44, 1226, 1937
BisI GCNGC 4 cut(s) 485, 493, 1224, 1494
BlnI CCTAGG 1 cut(s) 553
BlsI GCNGC 4 cut(s) 486, 494, 1225, 1495
BmcAI AGTACT 1 cut(s) 838
Bme1390I CCNGG 3 cut(s) 240, 1326, 1356
Bme18I GGWCC 6 cut(s) 890, 986, 1454, 1746, 1982, 2024
BmgT120I GGNCC 9 cut(s) 673, 857, 890, 986, 1186, 1454, 1746, 1982, 2024
BmiI GGNNCC 4 cut(s) 858, 891, 1515, 1983
BmrFI CCNGG 3 cut(s) 240, 1326, 1356
BmrI ACTGGG 2 cut(s) 331, 1987
BmsI GCATC 3 cut(s) 159, 796, 1485
BmuI ACTGGG 2 cut(s) 331, 1987
BpiI GAAGAC 2 cut(s) 1119, 1743
BpmI CTGGAG 3 cut(s) 261, 742, 1748
Bpu10I CCTNAGC 1 cut(s) 669
Bpu14I TTCGAA 1 cut(s) 1437
BpuEI CTTGAG 1 cut(s) 1266
BpuMI CCSGG 2 cut(s) 1326, 1356
BsaBI GATNNNNATC 2 cut(s) 1475, 1518
BsaI GGTCTC 1 cut(s) 654
BsaJI CCNNGG 3 cut(s) 436, 553, 1325
BsaWI WCCGGW 2 cut(s) 1510, 1748
BsaXI ACNNNNNCTCC 6 cut(s) 16, 46, 234, 264, 740, 770
Bsc4I CCNNNNNNNGG 4 cut(s) 670, 1451, 1875, 1991
Bse118I RCCGGY 1 cut(s) 1991
Bse1I ACTGG 6 cut(s) 326, 759, 1765, 1904, 1982, 2022
Bse3DI GCAATG 1 cut(s) 23
Bse8I GATNNNNATC 2 cut(s) 1475, 1518
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 3 cut(s) 436, 553, 1325
BseGI GGATG 4 cut(s) 745, 828, 1476, 2010
BseJI GATNNNNATC 2 cut(s) 1475, 1518
BseLI CCNNNNNNNGG 4 cut(s) 670, 1451, 1875, 1991
BseMI GCAATG 1 cut(s) 23
BseMII CTCAG 4 cut(s) 63, 683, 831, 1118
BseNI ACTGG 6 cut(s) 326, 759, 1765, 1904, 1982, 2022
BseRI GAGGAG 3 cut(s) 20, 37, 258
BseXI GCAGC 2 cut(s) 496, 1210
BseYI CCCAGC 1 cut(s) 497
BsgI GTGCAG 2 cut(s) 783, 1209
Bsh1236I CGCG 5 cut(s) 495, 654, 687, 1230, 1238
Bsh1285I CGRYCG 2 cut(s) 1325, 1486
BshFI GGCC 7 cut(s) 492, 541, 674, 859, 1090, 1188, 1784
BsiEI CGRYCG 2 cut(s) 1325, 1486
BsiHKAI GWGCWC 2 cut(s) 130, 156
BsiSI CCGG 6 cut(s) 542, 1325, 1356, 1511, 1749, 1992
BslFI GGGAC 3 cut(s) 903, 1382, 1995
BslI CCNNNNNNNGG 4 cut(s) 670, 1451, 1875, 1991
BsmAI GTCTC 3 cut(s) 95, 337, 654
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 3 cut(s) 903, 1382, 1995
BsmI GAATGC 2 cut(s) 1033, 1262
BsnI GGCC 7 cut(s) 492, 541, 674, 859, 1090, 1188, 1784
Bso31I GGTCTC 1 cut(s) 654
Bsp119I TTCGAA 1 cut(s) 1437
Bsp1286I GDGCHC 2 cut(s) 130, 156
Bsp143I GATC 8 cut(s) 325, 649, 825, 1139, 1241, 1483, 1513, 1618
Bsp68I TCGCGA 2 cut(s) 654, 687
BspACI CCGC 5 cut(s) 493, 854, 1230, 1238, 1494
BspANI GGCC 7 cut(s) 492, 541, 674, 859, 1090, 1188, 1784
BspCNI CTCAG 4 cut(s) 62, 682, 832, 1117
BspFNI CGCG 5 cut(s) 495, 654, 687, 1230, 1238
BspLI GGNNCC 4 cut(s) 858, 891, 1515, 1983
BspMAI CTGCAG 1 cut(s) 1803
BspMI ACCTGC 1 cut(s) 1792
BspPI GGATC 5 cut(s) 333, 1147, 1249, 1508, 1521
BspQI GCTCTTC 1 cut(s) 74
BspT104I TTCGAA 1 cut(s) 1437
BspTNI GGTCTC 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 23
BsrFI RCCGGY 1 cut(s) 1991
BsrI ACTGG 6 cut(s) 326, 759, 1765, 1904, 1982, 2022
BssAI RCCGGY 1 cut(s) 1991
BssECI CCNNGG 3 cut(s) 436, 553, 1325
BssMI GATC 8 cut(s) 325, 649, 825, 1139, 1241, 1483, 1513, 1618
BssSI CACGAG 1 cut(s) 1181
BssT1I CCWWGG 1 cut(s) 553
Bst2BI CACGAG 1 cut(s) 1181
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 7 cut(s) 196, 788, 907, 1351, 1538, 1633, 1694
Bst6I CTCTTC 1 cut(s) 74
BstAPI GCANNNNNTGC 1 cut(s) 1205
BstBI TTCGAA 1 cut(s) 1437
BstC8I GCNNGC 3 cut(s) 126, 1228, 1993
BstDEI CTNAG 7 cut(s) 49, 131, 669, 840, 914, 1104, 1420
BstF5I GGATG 4 cut(s) 745, 828, 1476, 2010
BstFNI CGCG 5 cut(s) 495, 654, 687, 1230, 1238
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 8 cut(s) 328, 652, 828, 1142, 1244, 1486, 1516, 1621
BstMAI GTCTC 3 cut(s) 95, 337, 654
BstMBI GATC 8 cut(s) 325, 649, 825, 1139, 1241, 1483, 1513, 1618
BstMCI CGRYCG 2 cut(s) 1325, 1486
BstMWI GCNNNNNNNGC 4 cut(s) 547, 865, 1205, 1790
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 3 cut(s) 238, 1324, 1354
BstSFI CTRYAG 3 cut(s) 810, 1333, 1799
BstUI CGCG 5 cut(s) 495, 654, 687, 1230, 1238
BstV1I GCAGC 2 cut(s) 496, 1210
BstV2I GAAGAC 2 cut(s) 1119, 1743
BstX2I RGATCY 2 cut(s) 325, 1513
BstXI CCANNNNNNTGG 1 cut(s) 1464
BstYI RGATCY 2 cut(s) 325, 1513
BsuI GTATCC 3 cut(s) 44, 1226, 1937
BsuRI GGCC 7 cut(s) 492, 541, 674, 859, 1090, 1188, 1784
BtsCI GGATG 4 cut(s) 745, 828, 1476, 2010
BtsIMutI CAGTG 5 cut(s) 766, 798, 903, 1545, 1915
BtuMI TCGCGA 2 cut(s) 654, 687
BveI ACCTGC 1 cut(s) 1792
Cac8I GCNNGC 3 cut(s) 126, 1228, 1993
CaiI CAGNNNCTG 1 cut(s) 1148
CfoI GCGC 1 cut(s) 497
Cfr10I RCCGGY 1 cut(s) 1991
Cfr13I GGNCC 9 cut(s) 673, 857, 890, 986, 1186, 1454, 1746, 1982, 2024
CseI GACGC 2 cut(s) 457, 515
Csp6I GTAC 7 cut(s) 837, 1093, 1174, 1378, 1695, 1897, 2002
CviQI GTAC 7 cut(s) 837, 1093, 1174, 1378, 1695, 1897, 2002
DdeI CTNAG 7 cut(s) 49, 131, 669, 840, 914, 1104, 1420
DpnI GATC 8 cut(s) 327, 651, 827, 1141, 1243, 1485, 1515, 1620
DpnII GATC 8 cut(s) 325, 649, 825, 1139, 1241, 1483, 1513, 1618
DraI TTTAAA 1 cut(s) 727
EaeI YGGCCR 4 cut(s) 490, 539, 1088, 1782
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 553
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 654
Eco47I GGWCC 6 cut(s) 890, 986, 1454, 1746, 1982, 2024
Eco53kI GAGCTC 1 cut(s) 154
EcoICRI GAGCTC 1 cut(s) 154
EcoO109I RGGNCCY 4 cut(s) 673, 1186, 1982, 2024
EcoRI GAATTC 2 cut(s) 258, 1884
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 553
EcoT22I ATGCAT 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 553
Esp3I CGTCTC 1 cut(s) 95
FalI AAGNNNNNCTT 4 cut(s) 681, 713, 1192, 1224
FaqI GGGAC 3 cut(s) 903, 1382, 1995
FauI CCCGC 2 cut(s) 847, 1231
FauNDI CATATG 1 cut(s) 511
FbaI TGATCA 1 cut(s) 825
Fnu4HI GCNGC 4 cut(s) 485, 493, 1224, 1494
FokI GGATG 4 cut(s) 752, 835, 1463, 1997
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 4 cut(s) 485, 493, 1224, 1494
FspBI CTAG 5 cut(s) 530, 554, 834, 1122, 1190
GlaI GCGC 1 cut(s) 496
GluI GCNGC 4 cut(s) 485, 493, 1224, 1494
GsaI CCCAGC 1 cut(s) 501
GsuI CTGGAG 3 cut(s) 261, 742, 1748
HaeIII GGCC 7 cut(s) 492, 541, 674, 859, 1090, 1188, 1784
HapII CCGG 6 cut(s) 542, 1325, 1356, 1511, 1749, 1992
HgaI GACGC 2 cut(s) 457, 515
HhaI GCGC 1 cut(s) 497
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 7 cut(s) 222, 567, 630, 1169, 1193, 1279, 1809
HpaII CCGG 6 cut(s) 542, 1325, 1356, 1511, 1749, 1992
HphI GGTGA 3 cut(s) 22, 452, 974
Hpy166II GTNNAC 4 cut(s) 466, 784, 1534, 1545
Hpy188I TCNGA 9 cut(s) 41, 221, 616, 1012, 1111, 1129, 1446, 1524, 2015
Hpy8I GTNNAC 4 cut(s) 466, 784, 1534, 1545
Hpy99I CGWCG 3 cut(s) 391, 659, 932
HpyAV CCTTC 9 cut(s) 114, 527, 953, 961, 970, 1045, 1444, 1852, 2015
HpyCH4III ACNGT 7 cut(s) 196, 788, 907, 1351, 1538, 1633, 1694
HpyCH4IV ACGT 3 cut(s) 927, 1135, 1740
HpyCH4V TGCA 9 cut(s) 484, 509, 550, 764, 1055, 1226, 1260, 1346, 1801
HpyF10VI GCNNNNNNNGC 4 cut(s) 547, 865, 1205, 1790
HpyF3I CTNAG 7 cut(s) 49, 131, 669, 840, 914, 1104, 1420
HpySE526I ACGT 3 cut(s) 927, 1135, 1740
HspAI GCGC 1 cut(s) 495
KroI GCCGGC 1 cut(s) 1991
KroNI GCCGGC 1 cut(s) 1993
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 8 cut(s) 325, 649, 825, 1139, 1241, 1483, 1513, 1618
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 3 cut(s) 199, 1130, 1767
Lsp1109I GCAGC 2 cut(s) 496, 1210
LweI GCATC 3 cut(s) 159, 796, 1485
MaeI CTAG 5 cut(s) 530, 554, 834, 1122, 1190
MaeII ACGT 3 cut(s) 927, 1135, 1740
MaeIII GTNAC 6 cut(s) 223, 317, 379, 920, 1155, 1675
MalI GATC 8 cut(s) 327, 651, 827, 1141, 1243, 1485, 1515, 1620
MboI GATC 8 cut(s) 325, 649, 825, 1139, 1241, 1483, 1513, 1618
MboII GAAGA 8 cut(s) 61, 64, 599, 639, 985, 1085, 1124, 1748
MfeI CAATTG 1 cut(s) 1395
MflI RGATCY 2 cut(s) 325, 1513
MhlI GDGCHC 2 cut(s) 130, 156
MlyI GAGTC 3 cut(s) 231, 561, 1273
MmeI TCCRAC 4 cut(s) 770, 1481, 1547, 2038
Mph1103I ATGCAT 1 cut(s) 511
MreI CGCCGGCG 1 cut(s) 1991
MroNI GCCGGC 1 cut(s) 1991
MroXI GAANNNNTTC 2 cut(s) 693, 1116
MseI TTAA 6 cut(s) 141, 414, 458, 701, 726, 1005
MslI CAYNNNNRTG 1 cut(s) 2035
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 6 cut(s) 542, 1325, 1356, 1511, 1749, 1992
MspR9I CCNGG 3 cut(s) 240, 1326, 1356
MunI CAATTG 1 cut(s) 1395
Mva1269I GAATGC 2 cut(s) 1033, 1262
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 5 cut(s) 495, 654, 687, 1230, 1238
MwoI GCNNNNNNNGC 4 cut(s) 547, 865, 1205, 1790
NaeI GCCGGC 1 cut(s) 1993
NciI CCSGG 2 cut(s) 1326, 1356
NdeI CATATG 1 cut(s) 511
NdeII GATC 8 cut(s) 325, 649, 825, 1139, 1241, 1483, 1513, 1618
NgoMIV GCCGGC 1 cut(s) 1991
NlaIV GGNNCC 4 cut(s) 858, 891, 1515, 1983
NmeAIII GCCGAG 1 cut(s) 1947
NmuCI GTSAC 3 cut(s) 223, 920, 1675
NruI TCGCGA 2 cut(s) 654, 687
NsiI ATGCAT 1 cut(s) 511
NspV TTCGAA 1 cut(s) 1437
OliI CACNNNNGTG 1 cut(s) 2035
PciSI GCTCTTC 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 1698
PctI GAATGC 2 cut(s) 1033, 1262
PdiI GCCGGC 1 cut(s) 1993
PdmI GAANNNNTTC 2 cut(s) 693, 1116
PfeI GAWTC 4 cut(s) 630, 1169, 1193, 1809
PfoI TCCNGGA 1 cut(s) 238
PkrI GCNGC 4 cut(s) 486, 494, 1225, 1495
Ple19I CGATCG 1 cut(s) 1486
PleI GAGTC 3 cut(s) 230, 561, 1273
PpsI GAGTC 3 cut(s) 230, 561, 1273
PpuMI RGGWCCY 2 cut(s) 1982, 2024
Psp124BI GAGCTC 1 cut(s) 156
Psp5II RGGWCCY 2 cut(s) 1982, 2024
Psp6I CCWGG 1 cut(s) 238
PspFI CCCAGC 1 cut(s) 497
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 4 cut(s) 858, 891, 1515, 1983
PspPI GGNCC 9 cut(s) 673, 857, 890, 986, 1186, 1454, 1746, 1982, 2024
PspPPI RGGWCCY 2 cut(s) 1982, 2024
PstI CTGCAG 1 cut(s) 1803
PstNI CAGNNNCTG 1 cut(s) 1148
PsuI RGATCY 2 cut(s) 325, 1513
PvuI CGATCG 1 cut(s) 1486
RruI TCGCGA 2 cut(s) 654, 687
RsaI GTAC 7 cut(s) 838, 1094, 1175, 1379, 1696, 1898, 2003
RsaNI GTAC 7 cut(s) 837, 1093, 1174, 1378, 1695, 1897, 2002
RseI CAYNNNNRTG 1 cut(s) 2035
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 74
SaqAI TTAA 6 cut(s) 141, 414, 458, 701, 726, 1005
SatI GCNGC 4 cut(s) 485, 493, 1224, 1494
Sau3AI GATC 8 cut(s) 325, 649, 825, 1139, 1241, 1483, 1513, 1618
Sau96I GGNCC 9 cut(s) 673, 857, 890, 986, 1186, 1454, 1746, 1982, 2024
ScaI AGTACT 1 cut(s) 838
SchI GAGTC 3 cut(s) 231, 561, 1273
ScrFI CCNGG 3 cut(s) 240, 1326, 1356
SduI GDGCHC 2 cut(s) 130, 156
SfaNI GCATC 3 cut(s) 159, 796, 1485
SfcI CTRYAG 3 cut(s) 810, 1333, 1799
SfuI TTCGAA 1 cut(s) 1437
SgrAI CRCCGGYG 1 cut(s) 1991
SinI GGWCC 6 cut(s) 890, 986, 1454, 1746, 1982, 2024
SmiMI CAYNNNNRTG 1 cut(s) 2035
SmlI CTYRAG 1 cut(s) 1281
SmoI CTYRAG 1 cut(s) 1281
SsiI CCGC 5 cut(s) 493, 854, 1230, 1238, 1494
SspI AATATT 1 cut(s) 1429
SspMI CTAG 5 cut(s) 530, 554, 834, 1122, 1190
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 238, 1324, 1354
StyI CCWWGG 1 cut(s) 553
TaaI ACNGT 7 cut(s) 196, 788, 907, 1351, 1538, 1633, 1694
TaiI ACGT 3 cut(s) 930, 1138, 1743
TaqII GACCGA 1 cut(s) 1476
TatI WGTACW 2 cut(s) 836, 1896
TauI GCSGC 2 cut(s) 495, 1496
TfiI GAWTC 4 cut(s) 630, 1169, 1193, 1809
Tru1I TTAA 6 cut(s) 141, 414, 458, 701, 726, 1005
Tru9I TTAA 6 cut(s) 141, 414, 458, 701, 726, 1005
TscAI CASTG 5 cut(s) 766, 805, 910, 1552, 1915
TseFI GTSAC 3 cut(s) 223, 920, 1675
TseI GCWGC 2 cut(s) 484, 1223
Tsp45I GTSAC 3 cut(s) 223, 920, 1675
TspDTI ATGAA 6 cut(s) 592, 622, 1086, 1146, 1351, 1877
TspGWI ACGGA 2 cut(s) 672, 1611
TspRI CASTG 5 cut(s) 766, 805, 910, 1552, 1915
VpaK11BI GGWCC 6 cut(s) 890, 986, 1454, 1746, 1982, 2024
XapI RAATTY 3 cut(s) 258, 593, 1884
XbaI TCTAGA 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
XmaJI CCTAGG 1 cut(s) 553
XmnI GAANNNNTTC 2 cut(s) 693, 1116
XspI CTAG 5 cut(s) 530, 554, 834, 1122, 1190
ZrmI AGTACT 1 cut(s) 838
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.