Rw2G003080

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000523
Physical Location & Seq
Forward (+)
2909598 .. 2935559
25962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003080.1

Sequence Viewer

Length: 1779 bp
ATGGGGTTATATGGTGACACTTTCCTAAACGGTTTATATTTACATATCGGGAAAGTAACGGTAAAACATGGAGTTGTGTTGGATAATAAGCTTGTTCAACTAACTTTTTCTAATCCTGGTGGGAATGTTATTGGAATAAGGTATGGAGGAATTGACAACTTACTTGAAATTAACAATGAGGAAGGTGATAGAGGAATACATGGAACACAATTTAAAGTTATCACGGCGAGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAATGCCAGCCTCCACAGGGCCACAGGGGCGTACATAATACAGCGAGGACGTTCCGGGTTCTATACCTATGCCATCTTTGACAGTCTTGAAGGGTGGCCGGGAGGGGACATGGAGACACTTAGAGTGGCTTACAAGCTTCATCAAGCCAAGTTTAAATATATGGCGGTATCAGACACTAGGCAAAGGTTTATGCCAACAGCCGATTACCGAGCACGCGGTAAGCCACTTGCATTCAAAGAAGCTGTTCTACTGAACAATGAGACGAGCAGTCCTGAATTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCACCACCGGTGGGATTTTGGATGATCACACCTAGTGCTGAGTTTCGTATGGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTGCCTAACTGGGTTTGCTTCTCATCATTATGTTGGTAAAGAAGTTGGGCTGACATTCCAAGAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTTTATCTATCTTAACTCGACAGCACGAAACTCAGATAATAGTCCTTCAACGATCCGTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCATACAATTTCATTAGTTCTCACGACTTTCCTTCTTCAGATCAACGTGGATCAGTTTCGGGCCAGTTACTAGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTCATTGAAAATATTAGACCCGGTAACTATAATTTGTATGCATCTGTTGTTCCGGGGATAGTTGGAGACTACAAATATAAGGTTGACATTACAATTGAACCAGGAAGTAAAATCCAATTGGCTAGTGTTACTTACAAACCTCCGAGAACCGGTACCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCGGAGTTCTACATACCGGATCCATATCCAACTCTCATGAACAAGTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAGTACGGGTTGTGGTCACGTTATTATGAGCGTTACCTTAACAATGACCTCATCTACCTTGTTGGTGCTAACAATTACCGTGAGGATTGGTTTTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGTATGTAGGAACTACATGGCAGATACAGTTTGAACTCAAGAATGTGGTCAATCCCGGAAACTATATACTTCAACTGGCCTTGGCATCAGCCACTTTTGCAGAATTGCAAGTTCGGGTTAACAATTTGAATGCCAAGCCACCTCTTTTTTCGACGGGGCTAATAGGCGACGACAATGCCATAGCAAGACTTGGAATTCATGGCATATACTGGTTATGGAGTATTGATGTACCGAGCACTCTACTACGCGAAGGAAATGGCAATTCACCTTTCCACGGAGTCATGTATGATTATATAAGACTAGAAGGGCTAGGACCTTCTGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

592

Amino Acids

66.82

Weight (kDa)

6.34

Isoelectric Point (pI)

27.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 18 - 186 1.8e-44 Rhamnogalacturonate lyase family
fn3_3 PF14686 326 - 388 7.6e-16 Polysaccharide lyase family 4, domain II
CBM-like PF14683 403 - 583 7.1e-37 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1196
AccB1I GGYRCC 1 cut(s) 1196
AccII CGCG 2 cut(s) 483, 1695
AciI CCGC 3 cut(s) 431, 483, 1244
AclWI GGATC 4 cut(s) 868, 988, 1256, 1269
AcoI YGGCCR 4 cut(s) 362, 579, 929, 1444
AcsI RAATTY 2 cut(s) 542, 1641
AcuI CTGAAG 1 cut(s) 951
AdeI CACNNNGTG 1 cut(s) 665
AfaI GTAC 7 cut(s) 299, 575, 880, 1198, 1292, 1325, 1677
AfiI CCNNNNNNNGG 4 cut(s) 283, 641, 1193, 1721
AgeI ACCGGT 3 cut(s) 637, 1193, 1199
AhdI GACNNNNNGTC 1 cut(s) 534
AjnI CCWGG 2 cut(s) 115, 1144
AluBI AGCT 4 cut(s) 91, 403, 509, 1241
AluI AGCT 4 cut(s) 91, 403, 509, 1241
Alw21I GWGCWC 2 cut(s) 481, 1685
Alw26I GTCTC 3 cut(s) 374, 521, 1104
AlwI GGATC 4 cut(s) 868, 988, 1256, 1269
AoxI GGCC 8 cut(s) 230, 285, 362, 579, 929, 991, 1444, 1524
ApeKI GCWGC 1 cut(s) 1241
ApoI RAATTY 2 cut(s) 542, 1641
AsiGI ACCGGT 3 cut(s) 637, 1193, 1199
Asp700I GAANNNNTTC 1 cut(s) 510
Asp718I GGTACC 1 cut(s) 1196
AspS9I GGNCC 8 cut(s) 285, 686, 700, 719, 815, 991, 1202, 1760
AsuC2I CCSGG 5 cut(s) 322, 366, 1065, 1098, 1503
AsuHPI GGTGA 7 cut(s) 26, 197, 250, 624, 697, 803, 1704
AvaII GGWCC 6 cut(s) 686, 700, 719, 815, 1202, 1760
BalI TGGCCA 1 cut(s) 931
BamHI GGATCC 1 cut(s) 1261
BanI GGYRCC 1 cut(s) 1196
BarI GAAGNNNNNNTAC 2 cut(s) 498, 530
Bbv12I GWGCWC 2 cut(s) 481, 1685
BbvI GCAGC 1 cut(s) 1228
BccI CCATC 1 cut(s) 347
BceAI ACGGC 1 cut(s) 240
BcgI CGANNNNNNTGC 2 cut(s) 1604, 1638
BciT130I CCWGG 2 cut(s) 117, 1146
BciVI GTATCC 1 cut(s) 998
BclI TGATCA 1 cut(s) 654
BcnI CCSGG 5 cut(s) 322, 366, 1065, 1098, 1503
BcoDI GTCTC 3 cut(s) 374, 521, 1104
BfaI CTAG 7 cut(s) 444, 663, 1001, 1167, 1428, 1748, 1757
BfuI GTATCC 1 cut(s) 998
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 1 cut(s) 1242
BlsI GCNGC 1 cut(s) 1243
BmcAI AGTACT 1 cut(s) 575
Bme1390I CCNGG 7 cut(s) 117, 322, 366, 1065, 1098, 1146, 1503
Bme18I GGWCC 6 cut(s) 686, 700, 719, 815, 1202, 1760
BmeRI GACNNNNNGTC 1 cut(s) 534
BmgT120I GGNCC 8 cut(s) 285, 686, 700, 719, 815, 991, 1202, 1760
BmiI GGNNCC 2 cut(s) 1198, 1263
BmrFI CCNGG 7 cut(s) 117, 322, 366, 1065, 1098, 1146, 1503
BmrI ACTGGG 1 cut(s) 744
BmsI GCATC 2 cut(s) 1094, 1541
BmuI ACTGGG 1 cut(s) 744
BpuEI CTTGAG 2 cut(s) 1404, 1469
BpuMI CCSGG 5 cut(s) 322, 366, 1065, 1098, 1503
BsaBI GATNNNNATC 2 cut(s) 567, 1266
BsaJI CCNNGG 4 cut(s) 582, 1097, 1527, 1720
BsaWI WCCGGW 4 cut(s) 637, 1193, 1199, 1258
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 641, 1193, 1721
Bse118I RCCGGY 4 cut(s) 637, 1193, 1199, 1446
Bse1I ACTGG 4 cut(s) 739, 994, 1527, 1661
Bse8I GATNNNNATC 2 cut(s) 567, 1266
BseBI CCWGG 2 cut(s) 117, 1146
BseDI CCNNGG 4 cut(s) 582, 1097, 1527, 1720
BseGI GGATG 2 cut(s) 565, 657
BseJI GATNNNNATC 2 cut(s) 567, 1266
BseLI CCNNNNNNNGG 4 cut(s) 283, 641, 1193, 1721
BseMII CTCAG 3 cut(s) 660, 867, 1758
BseNI ACTGG 4 cut(s) 739, 994, 1527, 1661
BseX3I CGGCCG 1 cut(s) 579
BseXI GCAGC 1 cut(s) 1228
Bsh1236I CGCG 2 cut(s) 483, 1695
Bsh1285I CGRYCG 1 cut(s) 582
BshFI GGCC 8 cut(s) 232, 287, 364, 581, 931, 993, 1446, 1526
BshNI GGYRCC 1 cut(s) 1196
BshTI ACCGGT 3 cut(s) 637, 1193, 1199
BsiEI CGRYCG 1 cut(s) 582
BsiHKAI GWGCWC 2 cut(s) 481, 1685
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 4 cut(s) 283, 641, 1193, 1721
BsmAI GTCTC 3 cut(s) 374, 521, 1104
BsmBI CGTCTC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 386
BsmI GAATGC 3 cut(s) 497, 1224, 1582
BsnI GGCC 8 cut(s) 232, 287, 364, 581, 931, 993, 1446, 1526
Bsp1286I GDGCHC 2 cut(s) 481, 1685
Bsp1407I TGTACA 1 cut(s) 1290
Bsp143I GATC 7 cut(s) 654, 873, 901, 970, 980, 1231, 1261
BspACI CCGC 3 cut(s) 431, 483, 1244
BspANI GGCC 8 cut(s) 232, 287, 364, 581, 931, 993, 1446, 1526
BspCNI CTCAG 3 cut(s) 661, 866, 1759
BspFNI CGCG 2 cut(s) 483, 1695
BspHI TCATGA 1 cut(s) 1278
BspLI GGNNCC 2 cut(s) 1198, 1263
BspPI GGATC 4 cut(s) 868, 988, 1256, 1269
BspT107I GGYRCC 1 cut(s) 1196
BsrFI RCCGGY 4 cut(s) 637, 1193, 1199, 1446
BsrGI TGTACA 1 cut(s) 1290
BsrI ACTGG 4 cut(s) 739, 994, 1527, 1661
BssAI RCCGGY 4 cut(s) 637, 1193, 1199, 1446
BssECI CCNNGG 4 cut(s) 582, 1097, 1527, 1720
BssMI GATC 7 cut(s) 654, 873, 901, 970, 980, 1231, 1261
BssT1I CCWWGG 1 cut(s) 1527
Bst2UI CCWGG 2 cut(s) 117, 1146
Bst4CI ACNGT 5 cut(s) 32, 61, 350, 1400, 1476
BstAUI TGTACA 1 cut(s) 1290
BstC8I GCNNGC 2 cut(s) 274, 481
BstDEI CTNAG 4 cut(s) 386, 669, 853, 1767
BstDSI CCRYGG 1 cut(s) 1720
BstF5I GGATG 2 cut(s) 565, 657
BstFNI CGCG 2 cut(s) 483, 1695
BstKTI GATC 7 cut(s) 657, 876, 904, 973, 983, 1234, 1264
BstMAI GTCTC 3 cut(s) 374, 521, 1104
BstMBI GATC 7 cut(s) 654, 873, 901, 970, 980, 1231, 1261
BstMCI CGRYCG 1 cut(s) 582
BstMWI GCNNNNNNNGC 2 cut(s) 293, 1544
BstNI CCWGG 2 cut(s) 117, 1146
BstSCI CCNGG 7 cut(s) 115, 320, 364, 1063, 1096, 1144, 1501
BstUI CGCG 2 cut(s) 483, 1695
BstV1I GCAGC 1 cut(s) 1228
BstX2I RGATCY 1 cut(s) 1261
BstYI RGATCY 1 cut(s) 1261
BstZI CGGCCG 1 cut(s) 579
BsuI GTATCC 1 cut(s) 998
BsuRI GGCC 8 cut(s) 232, 287, 364, 581, 931, 993, 1446, 1526
BtgI CCRYGG 1 cut(s) 1720
BtsCI GGATG 2 cut(s) 565, 657
Cac8I GCNNGC 2 cut(s) 274, 481
CciI TCATGA 1 cut(s) 1278
Cfr10I RCCGGY 4 cut(s) 637, 1193, 1199, 1446
Cfr13I GGNCC 8 cut(s) 285, 686, 700, 719, 815, 991, 1202, 1760
Csp6I GTAC 7 cut(s) 298, 574, 879, 1197, 1291, 1324, 1676
CspAI ACCGGT 3 cut(s) 637, 1193, 1199
CviAII CATG 8 cut(s) 68, 200, 376, 605, 1279, 1464, 1646, 1729
CviQI GTAC 7 cut(s) 298, 574, 879, 1197, 1291, 1324, 1676
DdeI CTNAG 4 cut(s) 386, 669, 853, 1767
DpnI GATC 7 cut(s) 656, 875, 903, 972, 982, 1233, 1263
DpnII GATC 7 cut(s) 654, 873, 901, 970, 980, 1231, 1261
DraI TTTAAA 2 cut(s) 214, 421
DraIII CACNNNGTG 1 cut(s) 665
DriI GACNNNNNGTC 1 cut(s) 534
EaeI YGGCCR 4 cut(s) 362, 579, 929, 1444
EagI CGGCCG 1 cut(s) 579
Eam1105I GACNNNNNGTC 1 cut(s) 534
EclXI CGGCCG 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 1527
Eco32I GATATC 1 cut(s) 595
Eco47I GGWCC 6 cut(s) 686, 700, 719, 815, 1202, 1760
Eco52I CGGCCG 1 cut(s) 579
Eco57I CTGAAG 1 cut(s) 951
EcoO109I RGGNCCY 2 cut(s) 700, 1760
EcoRI GAATTC 1 cut(s) 1641
EcoRII CCWGG 2 cut(s) 115, 1144
EcoRV GATATC 1 cut(s) 595
EcoT14I CCWWGG 1 cut(s) 1527
EcoT22I ATGCAT 1 cut(s) 1087
ErhI CCWWGG 1 cut(s) 1527
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 8 cut(s) 71, 203, 379, 608, 1282, 1467, 1649, 1732
FaqI GGGAC 1 cut(s) 386
FatI CATG 8 cut(s) 67, 199, 375, 604, 1278, 1463, 1645, 1728
FbaI TGATCA 1 cut(s) 654
Fnu4HI GCNGC 1 cut(s) 1242
FokI GGATG 2 cut(s) 572, 664
Fsp4HI GCNGC 1 cut(s) 1242
FspBI CTAG 7 cut(s) 444, 663, 1001, 1167, 1428, 1748, 1757
GluI GCNGC 1 cut(s) 1242
HaeIII GGCC 8 cut(s) 232, 287, 364, 581, 931, 993, 1446, 1526
Hin1II CATG 8 cut(s) 71, 203, 379, 608, 1282, 1467, 1649, 1732
HincII GTYRAC 2 cut(s) 1129, 1567
HindII GTYRAC 2 cut(s) 1129, 1567
HindIII AAGCTT 2 cut(s) 89, 401
HinfI GANTC 3 cut(s) 1018, 1300, 1725
HpaI GTTAAC 1 cut(s) 1567
HphI GGTGA 7 cut(s) 26, 197, 250, 624, 697, 803, 1704
Hpy166II GTNNAC 3 cut(s) 881, 1129, 1567
Hpy188I TCNGA 6 cut(s) 439, 815, 856, 970, 1188, 1768
Hpy188III TCNNGA 7 cut(s) 49, 353, 539, 953, 1229, 1279, 1486
Hpy8I GTNNAC 3 cut(s) 881, 1129, 1567
Hpy99I CGWCG 2 cut(s) 1603, 1619
HpyAV CCTTC 8 cut(s) 176, 350, 542, 876, 972, 1691, 1745, 1773
HpyCH4III ACNGT 5 cut(s) 32, 61, 350, 1400, 1476
HpyCH4IV ACGT 4 cut(s) 316, 714, 976, 1339
HpyCH4V TGCA 5 cut(s) 497, 604, 1085, 1547, 1555
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 1544
HpyF3I CTNAG 4 cut(s) 386, 669, 853, 1767
HpySE526I ACGT 4 cut(s) 316, 714, 976, 1339
Hsp92II CATG 8 cut(s) 71, 203, 379, 608, 1282, 1467, 1649, 1732
KpnI GGTACC 1 cut(s) 1200
Ksp22I TGATCA 1 cut(s) 654
KspAI GTTAAC 1 cut(s) 1567
Kzo9I GATC 7 cut(s) 654, 873, 901, 970, 980, 1231, 1261
Lsp1109I GCAGC 1 cut(s) 1228
LweI GCATC 2 cut(s) 1094, 1541
MaeI CTAG 7 cut(s) 444, 663, 1001, 1167, 1428, 1748, 1757
MaeII ACGT 4 cut(s) 316, 714, 976, 1339
MaeIII GTNAC 8 cut(s) 14, 55, 996, 1067, 1171, 1335, 1352, 1423
MalI GATC 7 cut(s) 656, 875, 903, 972, 982, 1233, 1263
MboI GATC 7 cut(s) 654, 873, 901, 970, 980, 1231, 1261
MboII GAAGA 2 cut(s) 926, 957
MfeI CAATTG 2 cut(s) 1137, 1160
MflI RGATCY 1 cut(s) 1261
MhlI GDGCHC 2 cut(s) 481, 1685
MlsI TGGCCA 1 cut(s) 931
MluNI TGGCCA 1 cut(s) 931
MlyI GAGTC 3 cut(s) 1012, 1309, 1734
MmeI TCCRAC 6 cut(s) 60, 590, 697, 1087, 1287, 1295
Mox20I TGGCCA 1 cut(s) 931
Mph1103I ATGCAT 1 cut(s) 1087
MroXI GAANNNNTTC 1 cut(s) 510
MscI TGGCCA 1 cut(s) 931
MseI TTAA 6 cut(s) 171, 213, 420, 834, 1359, 1566
MslI CAYNNNNRTG 1 cut(s) 753
Msp20I TGGCCA 1 cut(s) 931
MspA1I CMGCKG 1 cut(s) 1241
MspR9I CCNGG 7 cut(s) 117, 322, 366, 1065, 1098, 1146, 1503
MunI CAATTG 2 cut(s) 1137, 1160
Mva1269I GAATGC 3 cut(s) 497, 1224, 1582
MvaI CCWGG 2 cut(s) 117, 1146
MvnI CGCG 2 cut(s) 483, 1695
MwoI GCNNNNNNNGC 2 cut(s) 293, 1544
NciI CCSGG 5 cut(s) 322, 366, 1065, 1098, 1503
NdeII GATC 7 cut(s) 654, 873, 901, 970, 980, 1231, 1261
NlaIII CATG 8 cut(s) 71, 203, 379, 608, 1282, 1467, 1649, 1732
NlaIV GGNNCC 2 cut(s) 1198, 1263
NmeAIII GCCGAG 2 cut(s) 557, 607
NmuCI GTSAC 3 cut(s) 14, 1335, 1423
NsiI ATGCAT 1 cut(s) 1087
PagI TCATGA 1 cut(s) 1278
PctI GAATGC 3 cut(s) 497, 1224, 1582
PdmI GAANNNNTTC 1 cut(s) 510
PfoI TCCNGGA 1 cut(s) 1501
PinAI ACCGGT 3 cut(s) 637, 1193, 1199
PkrI GCNGC 1 cut(s) 1243
PleI GAGTC 3 cut(s) 1012, 1308, 1733
PpsI GAGTC 3 cut(s) 1012, 1308, 1733
PpuMI RGGWCCY 2 cut(s) 700, 1760
Psp5II RGGWCCY 2 cut(s) 700, 1760
Psp6I CCWGG 2 cut(s) 115, 1144
PspGI CCWGG 2 cut(s) 115, 1144
PspN4I GGNNCC 2 cut(s) 1198, 1263
PspPI GGNCC 8 cut(s) 285, 686, 700, 719, 815, 991, 1202, 1760
PspPPI RGGWCCY 2 cut(s) 700, 1760
PsrI GAACNNNNNNTAC 2 cut(s) 1274, 1306
PsuI RGATCY 1 cut(s) 1261
PvuII CAGCTG 1 cut(s) 1241
RsaI GTAC 7 cut(s) 299, 575, 880, 1198, 1292, 1325, 1677
RsaNI GTAC 7 cut(s) 298, 574, 879, 1197, 1291, 1324, 1676
RseI CAYNNNNRTG 1 cut(s) 753
SaqAI TTAA 6 cut(s) 171, 213, 420, 834, 1359, 1566
SatI GCNGC 1 cut(s) 1242
Sau3AI GATC 7 cut(s) 654, 873, 901, 970, 980, 1231, 1261
Sau96I GGNCC 8 cut(s) 285, 686, 700, 719, 815, 991, 1202, 1760
ScaI AGTACT 1 cut(s) 575
SchI GAGTC 3 cut(s) 1012, 1309, 1734
ScrFI CCNGG 7 cut(s) 117, 322, 366, 1065, 1098, 1146, 1503
SduI GDGCHC 2 cut(s) 481, 1685
SfaNI GCATC 2 cut(s) 1094, 1541
SgrAI CRCCGGYG 1 cut(s) 637
SinI GGWCC 6 cut(s) 686, 700, 719, 815, 1202, 1760
SmiMI CAYNNNNRTG 1 cut(s) 753
SmlI CTYRAG 2 cut(s) 1419, 1484
SmoI CTYRAG 2 cut(s) 1419, 1484
SsiI CCGC 3 cut(s) 431, 483, 1244
SspI AATATT 1 cut(s) 1057
SspMI CTAG 7 cut(s) 444, 663, 1001, 1167, 1428, 1748, 1757
StyD4I CCNGG 7 cut(s) 115, 320, 364, 1063, 1096, 1144, 1501
StyI CCWWGG 1 cut(s) 1527
TaaI ACNGT 5 cut(s) 32, 61, 350, 1400, 1476
TaiI ACGT 4 cut(s) 319, 717, 979, 1342
TaqI TCGA 2 cut(s) 839, 1598
TatI WGTACW 2 cut(s) 573, 1290
Tru1I TTAA 6 cut(s) 171, 213, 420, 834, 1359, 1566
Tru9I TTAA 6 cut(s) 171, 213, 420, 834, 1359, 1566
TseFI GTSAC 3 cut(s) 14, 1335, 1423
TseI GCWGC 1 cut(s) 1241
Tsp45I GTSAC 3 cut(s) 14, 1335, 1423
TspDTI ATGAA 6 cut(s) 244, 395, 931, 1044, 1295, 1634
TspGWI ACGGA 2 cut(s) 866, 1737
VpaK11BI GGWCC 6 cut(s) 686, 700, 719, 815, 1202, 1760
XapI RAATTY 2 cut(s) 542, 1641
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 7 cut(s) 444, 663, 1001, 1167, 1428, 1748, 1757
ZrmI AGTACT 1 cut(s) 575
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.