FvH4_1g19194

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000523
Physical Location & Seq
Forward (+)
11410709 .. 11416195
5487 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19194.t5

Sequence Viewer

Length: 2061 bp
ATGGAGAAGATGAGGCAATGGGGTTGGCTTCTGGGGTCATTTGCAGCAATGCTTCTTCTGGTTTTGTTCTTGGCTCGTGATGGAAGTTACGACGAGACGACAAACCCAGTAAGACGATCACTGAGAAGCATAATTCCTTGGCAACAACATGGTGTCAGTTTAACAGCAAAGAACCGCAATAGAGTTGTTTTGGATAATGGCATTGTCAAACTCATTTTCTCTAATCCGGAGGGAAACGTTATTGGAATACAATACAAAGGCCTTGACATACTTGAAATGGGGAATGAAGAAGATAATAGAGGGTACTGGGATTTGGTCTGGAAAAAGAATACAACTGACAAACTACAAGCAACAAAATTTAAGGTGATTACCGCGAGGCCAGACCAAGCAGAAATTTCTTTCCTAAGAACATATGATGTTTCCCTTGGTAACGGCACGAACATAATTCCCTTGACCGTAGACAAAAGGTACATATTGCGGAGGGGTGCTTCTGGTTTCTATGCATATGGAATATTCGAACGCCTTGAAGGGTGGCCGGGAGTTCAGGATATGGATCAAATTAGAATGGTTTTCAAGCTTGAACAAGCCAAATTTGATTATATGGCGTTATCAGACACTAGGCAAAGAATGATGCCAACAGCAGCGGACCGTGGTAATGGTGAGCAACTTGCGTATAAGGAAGCTGTTCTTTTGACAGATCCAAGCAATCCTGAGTTTAGAGGAGAGGTAGATGACAAGTACCAGTACTCAATCGAGGACAAAGATAACAAAGTTCATGGTTGGATTAGTACTGATGAATCTGTGGGATTCTGGATGATCACACCCAGTGATGAATTTCGAACAGGAGGCCCCTTTAAGCAGGACCTCACCTCCCATGTTGGCCCAACTACCCTCTCGATGTTTGTTAGTACTCATTACGCTGGGAAGGATCTTGGGATCAAATTCGAAGAAGGTGAGGCATGGAAAAAGGTTTTTGGACCCGTCTTTGTGTATCTTAACTCGGCTCCAAGCTCAAAGAATTCTACCAGTATACTTTGGAATAATGCTAAACAGCAGCTGTTTAAAGAAGTCGAGATCTGGCCGTATAATTTCACTCAATCCGAGGACTTTCCTTCTTCTGATCAAAGGGGATCGGTTTCCGGCCAGTTACAAATACATGATCGGTACATCAATGAGAGCCTTCTCCCAGCAAGTTCTGGCTATGTAGGATTGGCTGCACCAGGAGAAGTGGGATCATGGCAAAGGGAAAACAAAGGTTATCAATTTTGGAATCAAGCCGACGAAGAAGGTAGATTCATTCTCAAAGATGTTCGACCCGGAAACTATAGTCTGTATGCATGGGTTCCTGGCGTTGTTGGGGATTACAAATATGAGGCTGATATTACAATTATTCCAGGGTGTAATATCAAACTGGACAATCTTACTTTCGAACCTCCAAGAACTGGTCCTACATTGTGGGAAATTGGCATCCCTGATCGAACTGCTGCCGAATTCTACGTACCAGATCCTTATCCGACTCTTTTGAACAATCTATACAACCACATACCCTCAGAAAACTTCAGGCAGTACGGGTTGTGGGCACGTTATGGAGAGCGATACCCTCATAGTGATCTTGTCTACACTGTAGGTGTCAACAATTACACTGATCACTGGTTCTATGCTCAAGTGACCAAGATTACTGGAGATGAAACATATGTAGGAACAACGTGGGAAGTTGTATTTGAACTTCATAATGTCACGAATCCAGGAATATATACACTCCAGTTGGCATTGGCCTCGGCACATAGTGCAGAATTGCAGGTTCGGATTAACAGCCAGAGCGACGAGAGACCTCTTTTCTCAACAAAGTTAATCGGAGGGGACAATGCGATAGCAAGACATGGAATCCATGGTCTGTACTGGTTGTTTAGTGTTGAGATACCGAGCTCGCTACTAAAGGAAGGAAACAACAGCGTGTACCTTTCTCAGACGAGAGGTGGTGAAACTTTCCAAGGAGTCATGTACGACTATATCAGACTTGAAGCACCTTCATCACACACTGACTCACAAGTAGTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

687

Amino Acids

77.96

Weight (kDa)

5.31

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 3 - 151 1.1e-60 Rhamnogalacturonate lyase family
fn3_3 PF14686 308 - 378 1.8e-23 Polysaccharide lyase family 4, domain II
CBM-like PF14683 392 - 581 8.8e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1789
AccB7I CCANNNNNTGG 1 cut(s) 1442
AccI GTMKAC 3 cut(s) 459, 1030, 1617
AccII CGCG 1 cut(s) 374
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 4 cut(s) 175, 372, 478, 644
AclI AACGTT 1 cut(s) 237
AclWI GGATC 7 cut(s) 561, 692, 936, 944, 1138, 1240, 1499
AcoI YGGCCR 3 cut(s) 533, 1079, 1141
AcsI RAATTY 7 cut(s) 356, 393, 590, 833, 941, 1018, 1490
AcuI CTGAAG 1 cut(s) 1543
AdeI CACNNNGTG 2 cut(s) 827, 1787
AfiI CCNNNNNNNGG 1 cut(s) 1442
AgsI TTSAA 7 cut(s) 275, 527, 574, 581, 1525, 1724, 2021
AjnI CCWGG 4 cut(s) 1219, 1345, 1393, 1744
AluBI AGCT 5 cut(s) 577, 683, 1011, 1057, 1926
AluI AGCT 5 cut(s) 577, 683, 1011, 1057, 1926
Alw21I GWGCWC 1 cut(s) 1928
Alw26I GTCTC 2 cut(s) 89, 1822
AlwI GGATC 7 cut(s) 561, 692, 936, 944, 1138, 1240, 1499
AlwNI CAGNNNCTG 1 cut(s) 1057
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 8 cut(s) 259, 377, 533, 847, 880, 1079, 1141, 1773
ApeKI GCWGC 5 cut(s) 44, 641, 1054, 1214, 1484
ApoI RAATTY 7 cut(s) 356, 393, 590, 833, 941, 1018, 1490
Asp700I GAANNNNTTC 1 cut(s) 684
AspS9I GGNCC 6 cut(s) 646, 848, 862, 881, 977, 1445
AsuC2I CCSGG 2 cut(s) 537, 1317
AsuHPI GGTGA 5 cut(s) 376, 671, 859, 965, 1991
AsuII TTCGAA 4 cut(s) 516, 838, 945, 1428
AvaII GGWCC 4 cut(s) 646, 862, 977, 1445
BaeGI GKGCMC 1 cut(s) 1582
BanII GRGCYC 1 cut(s) 1928
BarI GAAGNNNNNNTAC 2 cut(s) 1710, 1742
BauI CACGAG 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 1928
BbvI GCAGC 5 cut(s) 56, 653, 1066, 1201, 1471
BccI CCATC 1 cut(s) 74
BceAI ACGGC 2 cut(s) 448, 1066
BcgI CGANNNNNNTGC 2 cut(s) 1758, 1792
BciT130I CCWGG 4 cut(s) 1221, 1347, 1395, 1746
BclI TGATCA 3 cut(s) 816, 1120, 1645
BcnI CCSGG 2 cut(s) 537, 1317
BcoDI GTCTC 2 cut(s) 89, 1822
BfaI CTAG 1 cut(s) 618
BfmI CTRYAG 2 cut(s) 1324, 1623
BfuAI ACCTGC 1 cut(s) 1789
BglII AGATCT 1 cut(s) 1074
BisI GCNGC 5 cut(s) 45, 642, 1055, 1215, 1485
BlsI GCNGC 5 cut(s) 46, 643, 1056, 1216, 1486
BmcAI AGTACT 3 cut(s) 746, 790, 910
Bme1390I CCNGG 6 cut(s) 537, 1221, 1317, 1347, 1395, 1746
Bme18I GGWCC 4 cut(s) 646, 862, 977, 1445
BmgT120I GGNCC 6 cut(s) 646, 848, 862, 881, 977, 1445
BmiI GGNNCC 4 cut(s) 850, 979, 1005, 1344
BmrFI CCNGG 6 cut(s) 537, 1221, 1317, 1347, 1395, 1746
BmrI ACTGGG 3 cut(s) 101, 316, 819
BmsI GCATC 2 cut(s) 621, 1476
BmuI ACTGGG 3 cut(s) 101, 316, 819
BpmI CTGGAG 2 cut(s) 1701, 1745
Bpu14I TTCGAA 4 cut(s) 516, 838, 945, 1428
BpuEI CTTGAG 1 cut(s) 1647
BpuMI CCSGG 2 cut(s) 537, 1317
BsaAI YACGTR 1 cut(s) 1498
BsaBI GATNNNNATC 2 cut(s) 552, 1509
BsaI GGTCTC 1 cut(s) 1822
BsaJI CCNNGG 8 cut(s) 137, 424, 649, 1101, 1394, 1776, 1888, 1990
BsaWI WCCGGW 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 854, 884
Bsc4I CCNNNNNNNGG 1 cut(s) 1442
Bse3DI GCAATG 2 cut(s) 23, 54
Bse8I GATNNNNATC 2 cut(s) 552, 1509
BseAI TCCGGA 1 cut(s) 226
BseBI CCWGG 4 cut(s) 1221, 1347, 1395, 1746
BseDI CCNNGG 8 cut(s) 137, 424, 649, 1101, 1394, 1776, 1888, 1990
BseGI GGATG 2 cut(s) 819, 1467
BseJI GATNNNNATC 2 cut(s) 552, 1509
BseLI CCNNNNNNNGG 1 cut(s) 1442
BseMI GCAATG 2 cut(s) 23, 54
BseMII CTCAG 4 cut(s) 113, 702, 1563, 1979
BseRI GAGGAG 1 cut(s) 735
BseSI GKGCMC 1 cut(s) 1582
BseXI GCAGC 5 cut(s) 56, 653, 1066, 1201, 1471
BseYI CCCAGC 2 cut(s) 920, 1186
BsgI GTGCAG 2 cut(s) 1200, 1809
Bsh1236I CGCG 1 cut(s) 374
BshFI GGCC 8 cut(s) 261, 379, 535, 849, 882, 1081, 1143, 1775
BsiHKAI GWGCWC 1 cut(s) 1928
BsiSI CCGG 4 cut(s) 227, 536, 1140, 1317
BslFI GGGAC 1 cut(s) 1874
BslI CCNNNNNNNGG 1 cut(s) 1442
BsmAI GTCTC 2 cut(s) 89, 1822
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 1874
BsnI GGCC 8 cut(s) 261, 379, 535, 849, 882, 1081, 1143, 1775
Bso31I GGTCTC 1 cut(s) 1822
Bsp119I TTCGAA 4 cut(s) 516, 838, 945, 1428
Bsp1286I GDGCHC 2 cut(s) 1582, 1928
Bsp13I TCCGGA 1 cut(s) 226
Bsp19I CCATGG 1 cut(s) 1888
BspACI CCGC 4 cut(s) 175, 372, 478, 644
BspANI GGCC 8 cut(s) 261, 379, 535, 849, 882, 1081, 1143, 1775
BspCNI CTCAG 4 cut(s) 114, 703, 1562, 1978
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 4 cut(s) 850, 979, 1005, 1344
BspMI ACCTGC 1 cut(s) 1789
BspPI GGATC 7 cut(s) 561, 692, 936, 944, 1138, 1240, 1499
BspT104I TTCGAA 4 cut(s) 516, 838, 945, 1428
BspTNI GGTCTC 1 cut(s) 1822
BsrDI GCAATG 2 cut(s) 23, 54
BssECI CCNNGG 8 cut(s) 137, 424, 649, 1101, 1394, 1776, 1888, 1990
BssNAI GTATAC 1 cut(s) 1031
BssSI CACGAG 1 cut(s) 75
BssT1I CCWWGG 4 cut(s) 137, 424, 1888, 1990
Bst1107I GTATAC 1 cut(s) 1031
Bst2BI CACGAG 1 cut(s) 75
Bst2UI CCWGG 4 cut(s) 1221, 1347, 1395, 1746
Bst4CI ACNGT 3 cut(s) 457, 650, 1624
BstAPI GCANNNNNTGC 1 cut(s) 1787
BstBAI YACGTR 1 cut(s) 1498
BstBI TTCGAA 4 cut(s) 516, 838, 945, 1428
BstC8I GCNNGC 1 cut(s) 1928
BstDEI CTNAG 5 cut(s) 122, 404, 711, 1549, 1965
BstDSI CCRYGG 2 cut(s) 649, 1888
BstF5I GGATG 2 cut(s) 819, 1467
BstFNI CGCG 1 cut(s) 374
BstMAI GTCTC 2 cut(s) 89, 1822
BstMWI GCNNNNNNNGC 1 cut(s) 1787
BstNI CCWGG 4 cut(s) 1221, 1347, 1395, 1746
BstSCI CCNGG 6 cut(s) 535, 1219, 1315, 1345, 1393, 1744
BstSFI CTRYAG 2 cut(s) 1324, 1623
BstSLI GKGCMC 1 cut(s) 1582
BstSNI TACGTA 1 cut(s) 1498
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 5 cut(s) 56, 653, 1066, 1201, 1471
BstX2I RGATCY 4 cut(s) 697, 928, 1074, 1504
BstYI RGATCY 4 cut(s) 697, 928, 1074, 1504
BstZ17I GTATAC 1 cut(s) 1031
BsuRI GGCC 8 cut(s) 261, 379, 535, 849, 882, 1081, 1143, 1775
BtgI CCRYGG 2 cut(s) 649, 1888
BtsCI GGATG 2 cut(s) 819, 1467
BtsIMutI CAGTG 6 cut(s) 119, 832, 1620, 1641, 1648, 2037
BveI ACCTGC 1 cut(s) 1789
Cac8I GCNNGC 1 cut(s) 1928
CaiI CAGNNNCTG 1 cut(s) 1057
Cfr13I GGNCC 6 cut(s) 646, 848, 862, 881, 977, 1445
CpoI CGGWCCG 1 cut(s) 646
CspI CGGWCCG 1 cut(s) 646
DdeI CTNAG 5 cut(s) 122, 404, 711, 1549, 1965
DraI TTTAAA 1 cut(s) 1063
DraIII CACNNNGTG 2 cut(s) 827, 1787
EaeI YGGCCR 3 cut(s) 533, 1079, 1141
Ecl136II GAGCTC 1 cut(s) 1926
Eco105I TACGTA 1 cut(s) 1498
Eco130I CCWWGG 4 cut(s) 137, 424, 1888, 1990
Eco147I AGGCCT 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 1928
Eco31I GGTCTC 1 cut(s) 1822
Eco47I GGWCC 4 cut(s) 646, 862, 977, 1445
Eco53kI GAGCTC 1 cut(s) 1926
Eco57I CTGAAG 1 cut(s) 1543
EcoICRI GAGCTC 1 cut(s) 1926
EcoO109I RGGNCCY 2 cut(s) 848, 862
EcoRI GAATTC 2 cut(s) 1018, 1490
EcoRII CCWGG 4 cut(s) 1219, 1345, 1393, 1744
EcoT14I CCWWGG 4 cut(s) 137, 424, 1888, 1990
EcoT22I ATGCAT 2 cut(s) 505, 1339
EcoT38I GRGCYC 1 cut(s) 1928
ErhI CCWWGG 4 cut(s) 137, 424, 1888, 1990
Esp3I CGTCTC 1 cut(s) 89
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FaqI GGGAC 1 cut(s) 1874
FauNDI CATATG 3 cut(s) 412, 505, 1693
FbaI TGATCA 3 cut(s) 816, 1120, 1645
FblI GTMKAC 3 cut(s) 459, 1030, 1617
Fnu4HI GCNGC 5 cut(s) 45, 642, 1055, 1215, 1485
FokI GGATG 2 cut(s) 826, 1454
FriOI GRGCYC 1 cut(s) 1928
Fsp4HI GCNGC 5 cut(s) 45, 642, 1055, 1215, 1485
FspBI CTAG 1 cut(s) 618
GluI GCNGC 5 cut(s) 45, 642, 1055, 1215, 1485
GsaI CCCAGC 2 cut(s) 924, 1190
GsuI CTGGAG 2 cut(s) 1701, 1745
HaeIII GGCC 8 cut(s) 261, 379, 535, 849, 882, 1081, 1143, 1775
HapII CCGG 4 cut(s) 227, 536, 1140, 1317
HincII GTYRAC 1 cut(s) 1633
HindII GTYRAC 1 cut(s) 1633
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 9 cut(s) 797, 807, 1270, 1293, 1516, 1741, 1884, 1995, 2042
HpaII CCGG 4 cut(s) 227, 536, 1140, 1317
HphI GGTGA 5 cut(s) 376, 671, 859, 965, 1991
Hpy166II GTNNAC 5 cut(s) 460, 1031, 1618, 1633, 1957
Hpy188I TCNGA 9 cut(s) 613, 1102, 1120, 1515, 1552, 1806, 1856, 1968, 2015
Hpy188III TCNNGA 9 cut(s) 77, 227, 319, 545, 710, 811, 895, 1072, 1738
Hpy8I GTNNAC 5 cut(s) 460, 1031, 1618, 1633, 1957
Hpy99I CGWCG 3 cut(s) 95, 1283, 1826
HpyAV CCTTC 8 cut(s) 521, 919, 944, 1122, 1190, 1280, 1934, 2037
HpyCH4III ACNGT 3 cut(s) 457, 650, 1624
HpyCH4IV ACGT 4 cut(s) 237, 1497, 1582, 1706
HpyCH4V TGCA 6 cut(s) 44, 503, 1217, 1337, 1790, 1798
HpyF10VI GCNNNNNNNGC 1 cut(s) 1787
HpyF3I CTNAG 5 cut(s) 122, 404, 711, 1549, 1965
HpySE526I ACGT 4 cut(s) 237, 1497, 1582, 1706
Kpn2I TCCGGA 1 cut(s) 226
Ksp22I TGATCA 3 cut(s) 816, 1120, 1645
LmnI GCTCC 1 cut(s) 1009
Lsp1109I GCAGC 5 cut(s) 56, 653, 1066, 1201, 1471
LweI GCATC 2 cut(s) 621, 1476
MaeI CTAG 1 cut(s) 618
MaeII ACGT 4 cut(s) 237, 1497, 1582, 1706
MaeIII GTNAC 5 cut(s) 86, 428, 1146, 1666, 1735
MboII GAAGA 7 cut(s) 19, 47, 299, 302, 959, 1107, 1295
MflI RGATCY 4 cut(s) 697, 928, 1074, 1504
MhlI GDGCHC 2 cut(s) 1582, 1928
MlyI GAGTC 3 cut(s) 1510, 2004, 2036
MmeI TCCRAC 2 cut(s) 761, 1538
Mph1103I ATGCAT 2 cut(s) 505, 1339
MroI TCCGGA 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 684
MseI TTAA 7 cut(s) 161, 360, 855, 996, 1062, 1809, 1850
MspA1I CMGCKG 2 cut(s) 644, 1057
MspI CCGG 4 cut(s) 227, 536, 1140, 1317
MspR9I CCNGG 6 cut(s) 537, 1221, 1317, 1347, 1395, 1746
MvaI CCWGG 4 cut(s) 1221, 1347, 1395, 1746
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 1 cut(s) 1787
NciI CCSGG 2 cut(s) 537, 1317
NcoI CCATGG 1 cut(s) 1888
NdeI CATATG 3 cut(s) 412, 505, 1693
NlaIV GGNNCC 4 cut(s) 850, 979, 1005, 1344
NmeAIII GCCGAG 2 cut(s) 980, 1757
NmuCI GTSAC 2 cut(s) 1666, 1735
NsiI ATGCAT 2 cut(s) 505, 1339
NspV TTCGAA 4 cut(s) 516, 838, 945, 1428
PceI AGGCCT 1 cut(s) 261
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 6 cut(s) 797, 807, 1270, 1293, 1741, 1884
PflMI CCANNNNNTGG 1 cut(s) 1442
PfoI TCCNGGA 1 cut(s) 1744
PkrI GCNGC 5 cut(s) 46, 643, 1056, 1216, 1486
PleI GAGTC 3 cut(s) 1510, 2003, 2036
PpsI GAGTC 3 cut(s) 1510, 2003, 2036
Ppu21I YACGTR 1 cut(s) 1498
PpuMI RGGWCCY 1 cut(s) 862
Psp124BI GAGCTC 1 cut(s) 1928
Psp1406I AACGTT 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 862
Psp6I CCWGG 4 cut(s) 1219, 1345, 1393, 1744
PspFI CCCAGC 2 cut(s) 920, 1186
PspGI CCWGG 4 cut(s) 1219, 1345, 1393, 1744
PspN4I GGNNCC 4 cut(s) 850, 979, 1005, 1344
PspPI GGNCC 6 cut(s) 646, 848, 862, 881, 977, 1445
PspPPI RGGWCCY 1 cut(s) 862
PsrI GAACNNNNNNTAC 4 cut(s) 1432, 1464, 1517, 1549
PstNI CAGNNNCTG 1 cut(s) 1057
PsuI RGATCY 4 cut(s) 697, 928, 1074, 1504
PvuII CAGCTG 1 cut(s) 1057
Rsr2I CGGWCCG 1 cut(s) 646
RsrII CGGWCCG 1 cut(s) 646
SacI GAGCTC 1 cut(s) 1928
SaqAI TTAA 7 cut(s) 161, 360, 855, 996, 1062, 1809, 1850
SatI GCNGC 5 cut(s) 45, 642, 1055, 1215, 1485
Sau96I GGNCC 6 cut(s) 646, 848, 862, 881, 977, 1445
ScaI AGTACT 3 cut(s) 746, 790, 910
SchI GAGTC 3 cut(s) 1510, 2004, 2036
ScrFI CCNGG 6 cut(s) 537, 1221, 1317, 1347, 1395, 1746
SduI GDGCHC 2 cut(s) 1582, 1928
SfaNI GCATC 2 cut(s) 621, 1476
SfcI CTRYAG 2 cut(s) 1324, 1623
SfuI TTCGAA 4 cut(s) 516, 838, 945, 1428
SinI GGWCC 4 cut(s) 646, 862, 977, 1445
SmlI CTYRAG 1 cut(s) 1662
SmoI CTYRAG 1 cut(s) 1662
SnaBI TACGTA 1 cut(s) 1498
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 4 cut(s) 175, 372, 478, 644
SspI AATATT 1 cut(s) 513
SspMI CTAG 1 cut(s) 618
SstI GAGCTC 1 cut(s) 1928
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 6 cut(s) 535, 1219, 1315, 1345, 1393, 1744
StyI CCWWGG 4 cut(s) 137, 424, 1888, 1990
TaaI ACNGT 3 cut(s) 457, 650, 1624
TaiI ACGT 4 cut(s) 240, 1500, 1585, 1709
TaqI TCGA 9 cut(s) 516, 753, 838, 896, 945, 1071, 1312, 1428, 1477
TatI WGTACW 4 cut(s) 744, 788, 908, 1896
TfiI GAWTC 6 cut(s) 797, 807, 1270, 1293, 1741, 1884
Tru1I TTAA 7 cut(s) 161, 360, 855, 996, 1062, 1809, 1850
Tru9I TTAA 7 cut(s) 161, 360, 855, 996, 1062, 1809, 1850
TscAI CASTG 6 cut(s) 126, 832, 1627, 1648, 1655, 2044
TseFI GTSAC 2 cut(s) 1666, 1735
TseI GCWGC 5 cut(s) 44, 641, 1054, 1214, 1484
Tsp45I GTSAC 2 cut(s) 1666, 1735
TspDTI ATGAA 8 cut(s) 300, 764, 810, 846, 1285, 1701, 1718, 2019
TspRI CASTG 6 cut(s) 126, 832, 1627, 1648, 1655, 2044
Van91I CCANNNNNTGG 1 cut(s) 1442
VpaK11BI GGWCC 4 cut(s) 646, 862, 977, 1445
XapI RAATTY 7 cut(s) 356, 393, 590, 833, 941, 1018, 1490
XmiI GTMKAC 3 cut(s) 459, 1030, 1617
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 1 cut(s) 618
ZrmI AGTACT 3 cut(s) 746, 790, 910
Zsp2I ATGCAT 2 cut(s) 505, 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.