Rorug02G0167800

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000523
Physical Location & Seq
Forward (+)
15067112 .. 15071330
4219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0167800.1

Sequence Viewer

Length: 2283 bp
ATGGCTCTGGATTTGGTCGGAGGATCACTGTTTACACTGCTCTATGACGGCGTTAAGCAAGCAATGGTGAAGACTACTCAGTTCAAATCCCTCCTTGCAGATCTCAAATCCACGCTAGACTCTTTGCAGCCAAGAATCATTGAGCAGATTGGAGAGCACAACACAGAATTGGGCCTCCCAAACGAGGAAATAGAAGATCTCAAAAGACAAATGGAGGAGGGAGTGAAGCTCGTTGGGAAGTTTTCCGAGTTTCGTGTGTGGAACTACTGCTGCGTAGGGGATTACACTGATCAACTTATTGAATTGGATAGGGCTCTTAGAAGACTGCTGGAAATGTTAAGGCTGCAGGAAGCGAGGGATGTGAAGGAGACTTTGCTTTTGTCGAGGCAAAACCGTGACAAACTTGATGAAGTGAATAGGAGGCTGATAGAGATACAGAAGTTGTTAGTGCAGCAGCAGCAGCAAGCAGGGGGTGTGACGGAGGCCACGGATTCAGGAAATAATACAGAGGTGGTTCCCGAGCCAATTCAAGGGAATGGCATACAAGTGGCTGAGTTGGATGGAGGAGATGCTCTAGAAGCAGTATTTGGGATGCTTTTTGATGCTATTTTAGAAGTGGAGGACAAGGCTGTGATGTTTAAAACCCCCCTTGGTGATCTCAAATCCACCCTAGATTCCTTAAAACCACTGATTGAAGAGATAGCACAACGTAACAAGGTATTGGTTCGCCCAAAGGAGGAGCTAGAAAAATTTAAGAAACAAATGGAGAAGGGTGTGGAGTTGATTCCCAAGTGTTCCAAGGTTCATCCCCGGGCCAGCTGCAAAAAGTCCACGTACACTGAAACACTTATTGAATTGGATAAGTTTCTTCAAAGACAGTTGTATATTCTCAAAATGCAGGTAGCAAGGGATGTGAGGAACACCTTAGTTTCGATAAGGAATATAGAGGTGGCAATTGAGCAAATTGAAAAGAGTGGTGTGCTACAAAATCAAGTTCAAACTGAAGGTTGTTCAACGGTATCTGGACCTTCGCCGCCTAAAGTTGGATTGGATGTAGTGAATGTGCAGGGAACAAGGGATGTGGAGGAGACAATGAGTTCAATAAAGCATACAGCAGGAGGGATCAAACCAGTTGAAGAGAGTGGTGTAGTACAAATTCAACTTGAAACTAAAGGAACCAAAAATGCTCCCAAGGATTACACACTACTCTCTCGGATATCCAGAGCTTTCAGCAGGAACATAGATTCGGACACTAGCAGTGGAGGAGGAACAAGTCTATTCTCGGAGGGAAAAACATCAACATTCAGAAGCAAAGATGATGATCAGTCAATTGAATGCCATATCTCGGAGACATCCAACCCAAGAGTTTTCAGTTTTGCAGAGTTGAAGACTGCTACAAGGAATTTCAGGGCAGACACAACGCTTAGTGAGGGAGGCTTTGGGAAGGTTTTCAAGGGCTGGTTGGATGAGACGACGCTTTCACCTTCTAAACCTGGCACTGGAATGGTGGTTGCTATCAAGAAACTGAACCCAGAAAGTTTTCAAGGCTTTGAAAAGTGGCAGCTGGCAGAGGTGAACTTTGGAGGAAGGCTTTCTCATCCCAACCTTACGAAGCTGTTGGGATACTGTTGGGAAGATAAGGAGTTACTCCTTGTTCATGAGTTCATGAGCAGAGGAAGCTTGGAGAACCATCTTTTCAGACGTCATCCAAACCTTGAACCACTATCTTGGGAAAAAAGAATCAAAATAGCTATCGGAGCAGCTCGAGGCCTGGCCTTCTTGCACAGTTTAGAAATATCAGTTATATACAGAGATTTTAAGACCTCAAATATATTGCTTGATGGGAATTACAATGCAAAACTCTCGGATTTGGGCTTGGCGAAATTGGGGCCAACTGGTGGAGACTCACTTGTGTCAACTAGGGTTATGAGGACATATGGTTATGCTGCTCCAGAATACATTGCAACAGGTCATTTGACCGTGAAGAGTGATGTGTATGCCTTTGGTGTTGTGCTGCTTGAACTTCTCACAGGGTTACGAGCACTCGATCCTAAACGCCCCAAGGAACAACATAATCTGGTAGAGTGGGCTAAACCACTTCTTTCAAATAAAAAAAAGTCGAAAACCATCATCGATGCGCAGATGGAAGGCCAATATTCCTTTAATGCAGCATCAAAGGCAGGACAGATTACTCTAAAATGTCTAGCACCACATCATACAGACCGACCTTCCATGAAAGAAGTTGTGGAGGAACTGGAACAGATTCAGGCAATCAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

760

Amino Acids

84.82

Weight (kDa)

6.56

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 137 1.9e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 184 - 319 7e-29 Arabidopsis broad-spectrum mildew resistance protein RPW8
PK_Tyr_Ser-Thr PF07714 471 - 752 1.2e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 473 - 750 1.4e-37 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1705
Acc16I TGCGCA 1 cut(s) 2139
Acc36I ACCTGC 1 cut(s) 889
AccB7I CCANNNNNTGG 1 cut(s) 1898
AciI CCGC 1 cut(s) 1034
AclWI GGATC 3 cut(s) 31, 1130, 2042
AcsI RAATTY 3 cut(s) 749, 1155, 1402
AcuI CTGAAG 1 cut(s) 1023
AcyI GRCGYC 1 cut(s) 1702
AfaI GTAC 2 cut(s) 836, 1152
AfiI CCNNNNNNNGG 7 cut(s) 184, 530, 736, 1043, 1345, 1499, 1898
AjnI CCWGG 2 cut(s) 1492, 1770
AjuI GAANNNNNNNTTGG 2 cut(s) 570, 602
AluBI AGCT 9 cut(s) 229, 742, 819, 1226, 1564, 1615, 1680, 1751, 1763
AluI AGCT 9 cut(s) 229, 742, 819, 1226, 1564, 1615, 1680, 1751, 1763
Alw21I GWGCWC 2 cut(s) 159, 2044
Alw26I GTCTC 5 cut(s) 362, 1082, 1343, 1463, 1896
AlwI GGATC 3 cut(s) 31, 1130, 2042
Ama87I CYCGRG 3 cut(s) 518, 810, 1764
AoxI GGCC 7 cut(s) 172, 483, 813, 1768, 1773, 1889, 2149
ApoI RAATTY 3 cut(s) 749, 1155, 1402
ArsI GACNNNNNNTTYG 2 cut(s) 361, 393
Asp700I GAANNNNTTC 4 cut(s) 1448, 1539, 1591, 2262
AspLEI GCGC 1 cut(s) 2140
AspS9I GGNCC 4 cut(s) 172, 813, 1025, 1889
AsuC2I CCSGG 2 cut(s) 811, 812
AsuHPI GGTGA 4 cut(s) 79, 665, 1473, 1585
AvaI CYCGRG 3 cut(s) 518, 810, 1764
AvaII GGWCC 1 cut(s) 1025
BaeI ACNNNNGTAYC 2 cut(s) 425, 458
BanII GRGCYC 1 cut(s) 316
BbsI GAAGAC 3 cut(s) 77, 328, 1394
Bbv12I GWGCWC 2 cut(s) 159, 2044
BccI CCATC 5 cut(s) 554, 1698, 1835, 2135, 2137
BceAI ACGGC 1 cut(s) 64
BcgI CGANNNNNNTGC 2 cut(s) 1845, 1879
BciT130I CCWGG 2 cut(s) 1494, 1772
BciVI GTATCC 1 cut(s) 1616
BclI TGATCA 2 cut(s) 289, 1321
BcnI CCSGG 2 cut(s) 811, 812
BcoDI GTCTC 5 cut(s) 362, 1082, 1343, 1463, 1896
BfaI CTAG 7 cut(s) 116, 575, 671, 743, 1254, 1920, 2204
BfmI CTRYAG 1 cut(s) 344
BfuAI ACCTGC 1 cut(s) 889
BfuI GTATCC 1 cut(s) 1616
BglII AGATCT 2 cut(s) 100, 196
Bme1390I CCNGG 4 cut(s) 811, 812, 1494, 1772
Bme18I GGWCC 1 cut(s) 1025
BmeT110I CYCGRG 3 cut(s) 518, 810, 1764
BmgT120I GGNCC 4 cut(s) 172, 813, 1025, 1889
BmiI GGNNCC 3 cut(s) 516, 1177, 1890
BmrFI CCNGG 4 cut(s) 811, 812, 1494, 1772
BmsI GCATC 5 cut(s) 559, 582, 592, 2125, 2180
BpiI GAAGAC 3 cut(s) 77, 328, 1394
BplI GAGNNNNNCTC 2 cut(s) 213, 245
BpmI CTGGAG 1 cut(s) 1935
BpuMI CCSGG 2 cut(s) 811, 812
Bsa29I ATCGAT 1 cut(s) 2133
BsaAI YACGTR 1 cut(s) 834
BsaBI GATNNNNATC 1 cut(s) 1320
BsaHI GRCGYC 1 cut(s) 1702
BsaJI CCNNGG 7 cut(s) 486, 649, 798, 809, 810, 1191, 2061
Bsc4I CCNNNNNNNGG 7 cut(s) 184, 530, 736, 1043, 1345, 1499, 1898
Bse1I ACTGG 4 cut(s) 1130, 1504, 1900, 2259
Bse3DI GCAATG 2 cut(s) 69, 1959
Bse8I GATNNNNATC 1 cut(s) 1320
BseBI CCWGG 2 cut(s) 1494, 1772
BseCI ATCGAT 1 cut(s) 2133
BseDI CCNNGG 7 cut(s) 486, 649, 798, 809, 810, 1191, 2061
BseJI GATNNNNATC 1 cut(s) 1320
BseLI CCNNNNNNNGG 7 cut(s) 184, 530, 736, 1043, 1345, 1499, 1898
BseMI GCAATG 2 cut(s) 69, 1959
BseMII CTCAG 2 cut(s) 92, 543
BseNI ACTGG 4 cut(s) 1130, 1504, 1900, 2259
BseRI GAGGAG 5 cut(s) 230, 579, 752, 1100, 1278
BsgI GTGCAG 2 cut(s) 470, 1085
BshFI GGCC 7 cut(s) 174, 485, 815, 1770, 1775, 1891, 2151
BshVI ATCGAT 1 cut(s) 2133
BsiHKAI GWGCWC 2 cut(s) 159, 2044
BsiHKCI CYCGRG 3 cut(s) 518, 810, 1764
BsiSI CCGG 1 cut(s) 811
BslI CCNNNNNNNGG 7 cut(s) 184, 530, 736, 1043, 1345, 1499, 1898
BsmAI GTCTC 5 cut(s) 362, 1082, 1343, 1463, 1896
BsmBI CGTCTC 1 cut(s) 1463
BsmI GAATGC 1 cut(s) 1340
BsnI GGCC 7 cut(s) 174, 485, 815, 1770, 1775, 1891, 2151
BsoBI CYCGRG 3 cut(s) 518, 810, 1764
Bsp1286I GDGCHC 3 cut(s) 159, 316, 2044
Bsp143I GATC 8 cut(s) 23, 100, 196, 289, 655, 1122, 1321, 2047
BspACI CCGC 1 cut(s) 1034
BspANI GGCC 7 cut(s) 174, 485, 815, 1770, 1775, 1891, 2151
BspCNI CTCAG 2 cut(s) 91, 544
BspDI ATCGAT 1 cut(s) 2133
BspHI TCATGA 2 cut(s) 1657, 1665
BspLI GGNNCC 3 cut(s) 516, 1177, 1890
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 889
BspPI GGATC 3 cut(s) 31, 1130, 2042
BsrDI GCAATG 2 cut(s) 69, 1959
BsrI ACTGG 4 cut(s) 1130, 1504, 1900, 2259
BssECI CCNNGG 7 cut(s) 486, 649, 798, 809, 810, 1191, 2061
BssMI GATC 8 cut(s) 23, 100, 196, 289, 655, 1122, 1321, 2047
BssNI GRCGYC 1 cut(s) 1702
BssT1I CCWWGG 4 cut(s) 649, 798, 1191, 2061
Bst2UI CCWGG 2 cut(s) 1494, 1772
Bst4CI ACNGT 7 cut(s) 30, 395, 879, 1018, 1628, 1787, 1981
Bst6I CTCTTC 3 cut(s) 690, 1131, 1979
BstACI GRCGYC 1 cut(s) 1702
BstBAI YACGTR 1 cut(s) 834
BstC8I GCNNGC 4 cut(s) 60, 465, 817, 1566
BstDEI CTNAG 5 cut(s) 78, 317, 552, 925, 1424
BstDSI CCRYGG 1 cut(s) 486
BstHHI GCGC 1 cut(s) 2140
BstKTI GATC 8 cut(s) 26, 103, 199, 292, 658, 1125, 1324, 2050
BstMAI GTCTC 5 cut(s) 362, 1082, 1343, 1463, 1896
BstMBI GATC 8 cut(s) 23, 100, 196, 289, 655, 1122, 1321, 2047
BstMWI GCNNNNNNNGC 6 cut(s) 457, 460, 578, 1677, 1757, 2177
BstNI CCWGG 2 cut(s) 1494, 1772
BstSCI CCNGG 4 cut(s) 809, 810, 1492, 1770
BstSFI CTRYAG 1 cut(s) 344
BstV2I GAAGAC 3 cut(s) 77, 328, 1394
BstX2I RGATCY 2 cut(s) 100, 196
BstXI CCANNNNNNTGG 1 cut(s) 1728
BstYI RGATCY 2 cut(s) 100, 196
Bsu15I ATCGAT 1 cut(s) 2133
BsuI GTATCC 1 cut(s) 1616
BsuRI GGCC 7 cut(s) 174, 485, 815, 1770, 1775, 1891, 2151
BsuTUI ATCGAT 1 cut(s) 2133
BtgI CCRYGG 1 cut(s) 486
BtsI GCAGTG 2 cut(s) 35, 1264
BtsIMutI CAGTG 7 cut(s) 26, 35, 285, 686, 837, 1264, 1497
BveI ACCTGC 1 cut(s) 889
Cac8I GCNNGC 4 cut(s) 60, 465, 817, 1566
CciI TCATGA 2 cut(s) 1657, 1665
CfoI GCGC 1 cut(s) 2140
Cfr13I GGNCC 4 cut(s) 172, 813, 1025, 1889
Cfr9I CCCGGG 1 cut(s) 810
ClaI ATCGAT 1 cut(s) 2133
CseI GACGC 1 cut(s) 1483
Csp6I GTAC 2 cut(s) 835, 1151
CspCI CAANNNNNGTGG 2 cut(s) 1062, 1097
CviAII CATG 3 cut(s) 1658, 1666, 2233
CviQI GTAC 2 cut(s) 835, 1151
DdeI CTNAG 5 cut(s) 78, 317, 552, 925, 1424
DpnI GATC 8 cut(s) 25, 102, 198, 291, 657, 1124, 1323, 2049
DpnII GATC 8 cut(s) 23, 100, 196, 289, 655, 1122, 1321, 2047
DraI TTTAAA 1 cut(s) 640
Eam1104I CTCTTC 3 cut(s) 690, 1131, 1979
EarI CTCTTC 3 cut(s) 690, 1131, 1979
Eco130I CCWWGG 4 cut(s) 649, 798, 1191, 2061
Eco147I AGGCCT 1 cut(s) 1770
Eco24I GRGCYC 1 cut(s) 316
Eco32I GATATC 1 cut(s) 1218
Eco47I GGWCC 1 cut(s) 1025
Eco57I CTGAAG 1 cut(s) 1023
Eco88I CYCGRG 3 cut(s) 518, 810, 1764
EcoRII CCWGG 2 cut(s) 1492, 1770
EcoRV GATATC 1 cut(s) 1218
EcoT14I CCWWGG 4 cut(s) 649, 798, 1191, 2061
EcoT38I GRGCYC 1 cut(s) 316
ErhI CCWWGG 4 cut(s) 649, 798, 1191, 2061
Esp3I CGTCTC 1 cut(s) 1463
FaeI CATG 3 cut(s) 1661, 1669, 2236
FatI CATG 3 cut(s) 1657, 1665, 2232
FauNDI CATATG 1 cut(s) 1936
FbaI TGATCA 2 cut(s) 289, 1321
FriOI GRGCYC 1 cut(s) 316
FspBI CTAG 7 cut(s) 116, 575, 671, 743, 1254, 1920, 2204
FspI TGCGCA 1 cut(s) 2139
GlaI GCGC 1 cut(s) 2139
GsuI CTGGAG 1 cut(s) 1935
HaeIII GGCC 7 cut(s) 174, 485, 815, 1770, 1775, 1891, 2151
HapII CCGG 1 cut(s) 811
HgaI GACGC 1 cut(s) 1483
HhaI GCGC 1 cut(s) 2140
Hin1I GRCGYC 1 cut(s) 1702
Hin1II CATG 3 cut(s) 1661, 1669, 2236
Hin6I GCGC 1 cut(s) 2138
HinP1I GCGC 1 cut(s) 2138
HincII GTYRAC 1 cut(s) 1917
HindII GTYRAC 1 cut(s) 1917
HindIII AAGCTT 1 cut(s) 1678
HinfI GANTC 9 cut(s) 119, 135, 491, 674, 784, 1244, 1740, 1904, 2263
HpaII CCGG 1 cut(s) 811
HphI GGTGA 4 cut(s) 79, 665, 1473, 1585
Hpy166II GTNNAC 5 cut(s) 33, 831, 837, 1576, 1917
Hpy8I GTNNAC 5 cut(s) 33, 831, 837, 1576, 1917
Hpy99I CGWCG 1 cut(s) 1477
HpyCH4III ACNGT 7 cut(s) 30, 395, 879, 1018, 1628, 1787, 1981
HpyCH4IV ACGT 3 cut(s) 709, 833, 1702
HpyF10VI GCNNNNNNNGC 6 cut(s) 457, 460, 578, 1677, 1757, 2177
HpyF3I CTNAG 5 cut(s) 78, 317, 552, 925, 1424
HpySE526I ACGT 3 cut(s) 709, 833, 1702
Hsp92I GRCGYC 1 cut(s) 1702
Hsp92II CATG 3 cut(s) 1661, 1669, 2236
HspAI GCGC 1 cut(s) 2138
Ksp22I TGATCA 2 cut(s) 289, 1321
Kzo9I GATC 8 cut(s) 23, 100, 196, 289, 655, 1122, 1321, 2047
LmnI GCTCC 4 cut(s) 739, 1192, 1757, 1954
LweI GCATC 5 cut(s) 559, 582, 592, 2125, 2180
MaeI CTAG 7 cut(s) 116, 575, 671, 743, 1254, 1920, 2204
MaeII ACGT 3 cut(s) 709, 833, 1702
MaeIII GTNAC 5 cut(s) 395, 475, 710, 1644, 2034
MalI GATC 8 cut(s) 25, 102, 198, 291, 657, 1124, 1323, 2049
MboI GATC 8 cut(s) 23, 100, 196, 289, 655, 1122, 1321, 2047
MboII GAAGA 9 cut(s) 82, 206, 333, 707, 860, 1148, 1399, 1646, 1996
MfeI CAATTG 2 cut(s) 954, 1329
MflI RGATCY 2 cut(s) 100, 196
MhlI GDGCHC 3 cut(s) 159, 316, 2044
MlyI GAGTC 2 cut(s) 113, 1898
MmeI TCCRAC 4 cut(s) 537, 1024, 1380, 1443
MroXI GAANNNNTTC 4 cut(s) 1448, 1539, 1591, 2262
MseI TTAA 7 cut(s) 54, 338, 639, 680, 753, 1818, 2163
MslI CAYNNNNRTG 2 cut(s) 545, 1502
MspA1I CMGCKG 2 cut(s) 819, 1564
MspI CCGG 1 cut(s) 811
MspR9I CCNGG 4 cut(s) 811, 812, 1494, 1772
MunI CAATTG 2 cut(s) 954, 1329
Mva1269I GAATGC 1 cut(s) 1340
MvaI CCWGG 2 cut(s) 1494, 1772
MwoI GCNNNNNNNGC 6 cut(s) 457, 460, 578, 1677, 1757, 2177
NciI CCSGG 2 cut(s) 811, 812
NdeI CATATG 1 cut(s) 1936
NdeII GATC 8 cut(s) 23, 100, 196, 289, 655, 1122, 1321, 2047
NlaIII CATG 3 cut(s) 1661, 1669, 2236
NlaIV GGNNCC 3 cut(s) 516, 1177, 1890
NmuCI GTSAC 2 cut(s) 395, 475
NsbI TGCGCA 1 cut(s) 2139
PaeR7I CTCGAG 1 cut(s) 1764
PagI TCATGA 2 cut(s) 1657, 1665
PceI AGGCCT 1 cut(s) 1770
PctI GAATGC 1 cut(s) 1340
PdmI GAANNNNTTC 4 cut(s) 1448, 1539, 1591, 2262
PfeI GAWTC 7 cut(s) 135, 491, 674, 784, 1244, 1740, 2263
PflMI CCANNNNNTGG 1 cut(s) 1898
PleI GAGTC 2 cut(s) 113, 1898
PpsI GAGTC 2 cut(s) 113, 1898
Ppu21I YACGTR 1 cut(s) 834
Psp6I CCWGG 2 cut(s) 1492, 1770
PspGI CCWGG 2 cut(s) 1492, 1770
PspN4I GGNNCC 3 cut(s) 516, 1177, 1890
PspPI GGNCC 4 cut(s) 172, 813, 1025, 1889
PspXI VCTCGAGB 1 cut(s) 1764
PstI CTGCAG 1 cut(s) 348
PsuI RGATCY 2 cut(s) 100, 196
PvuII CAGCTG 2 cut(s) 819, 1564
RsaI GTAC 2 cut(s) 836, 1152
RsaNI GTAC 2 cut(s) 835, 1151
RseI CAYNNNNRTG 2 cut(s) 545, 1502
SaqAI TTAA 7 cut(s) 54, 338, 639, 680, 753, 1818, 2163
Sau3AI GATC 8 cut(s) 23, 100, 196, 289, 655, 1122, 1321, 2047
Sau96I GGNCC 4 cut(s) 172, 813, 1025, 1889
SchI GAGTC 2 cut(s) 113, 1898
ScrFI CCNGG 4 cut(s) 811, 812, 1494, 1772
SduI GDGCHC 3 cut(s) 159, 316, 2044
SfaNI GCATC 5 cut(s) 559, 582, 592, 2125, 2180
SfcI CTRYAG 1 cut(s) 344
Sfr274I CTCGAG 1 cut(s) 1764
SinI GGWCC 1 cut(s) 1025
SlaI CTCGAG 1 cut(s) 1764
SmaI CCCGGG 1 cut(s) 812
SmiMI CAYNNNNRTG 2 cut(s) 545, 1502
SmlI CTYRAG 1 cut(s) 1764
SmoI CTYRAG 1 cut(s) 1764
SseBI AGGCCT 1 cut(s) 1770
SsiI CCGC 1 cut(s) 1034
SspI AATATT 1 cut(s) 2156
SspMI CTAG 7 cut(s) 116, 575, 671, 743, 1254, 1920, 2204
StuI AGGCCT 1 cut(s) 1770
StyD4I CCNGG 4 cut(s) 809, 810, 1492, 1770
StyI CCWWGG 4 cut(s) 649, 798, 1191, 2061
TaaI ACNGT 7 cut(s) 30, 395, 879, 1018, 1628, 1787, 1981
TaiI ACGT 3 cut(s) 712, 836, 1705
TaqI TCGA 6 cut(s) 383, 932, 1765, 2046, 2120, 2133
TaqII GACCGA 1 cut(s) 2238
TatI WGTACW 1 cut(s) 1150
TauI GCSGC 1 cut(s) 1036
TfiI GAWTC 7 cut(s) 135, 491, 674, 784, 1244, 1740, 2263
Tru1I TTAA 7 cut(s) 54, 338, 639, 680, 753, 1818, 2163
Tru9I TTAA 7 cut(s) 54, 338, 639, 680, 753, 1818, 2163
TscAI CASTG 7 cut(s) 33, 42, 292, 693, 844, 1264, 1504
TseFI GTSAC 2 cut(s) 395, 475
Tsp45I GTSAC 2 cut(s) 395, 475
TspDTI ATGAA 5 cut(s) 423, 794, 1646, 1654, 2249
TspGWI ACGGA 2 cut(s) 494, 503
TspMI CCCGGG 1 cut(s) 810
TspRI CASTG 7 cut(s) 33, 42, 292, 693, 844, 1264, 1504
Van91I CCANNNNNTGG 1 cut(s) 1898
VpaK11BI GGWCC 1 cut(s) 1025
XapI RAATTY 3 cut(s) 749, 1155, 1402
XbaI TCTAGA 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 1764
XmaI CCCGGG 1 cut(s) 810
XmnI GAANNNNTTC 4 cut(s) 1448, 1539, 1591, 2262
XspI CTAG 7 cut(s) 116, 575, 671, 743, 1254, 1920, 2204
ZraI GACGTC 1 cut(s) 1703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.