Rorug02G0166800

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000523
Physical Location & Seq
Forward (+)
14968449 .. 14972179
3731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0166800.1

Sequence Viewer

Length: 2637 bp
ATGGTTGGTGGAGGTGATGTTTTTGGGGGGGCTGCTGGAGGATTGCTGTTCAATATTCTCTATGATGTGCTTAAAGAGCTCATCAGCAAGTCTATGATATATAGACCTCTCCTCCAAAACATTCTATCTGCGCTAGACTTATATGGACCAGTGATCAAACAGATAGAAGCAAGCAATGAGGAACTGGATCTTCCAAAGGAGGAAGTAGAAAATTTCAAAGCACATATGGAGAATGGCATAGAGATAGTCCAGAAGTGCTCTAAGGTTCGCAAGTGGAACATCTACAAGAAGTACAAGTATGCCAACAAACTTCTTGGATGGGATGCTGCTCTTGTAAGACAGTTGGATGTACTGAAGGCGCAGGGAGTAAGGGATGGGAAGAAGACCTTGGTTTCGGTAAAGCAGATAGAGAGGGTGGTCACTGGGATTGAAATGAGTAGTATCATTTTAGAGGCAGGGGTTGAGCATGTTGCAATAACCAGTAGGCATTTGGAGAAGGCGCTCAGCGGAGTTGAAATGAGAGGTGTAGATATAGTGGAGGGGATGTACCGAATTGAAGGCCGAATTGAAACAAGCTCTAGCATTGTGGAGGCGGGGTTCAAGCGGATTGAAGACCGACTTAATACACGAAATGGAGCTAAAGTTGGAGCTTGGTCTGTTGTGCCTGAACTTGCCCAGTTTACGGTTGGATTGGATGAGCCTTTGAAGGAATTGAAGATGAAACTTCTTAAGGATGGAAACTCGATGCTAGTGGTCACTGCTCCCGGAGGATGTGGGAAAACTACATTGGCAACAAAGTTTTGTCAGGATGAGCAAGTGAAAGATAAATTCAAGAATAATATCTACTTTGTCACTGTGTCAAACACACCAAACCTGAGCCATATTGTGCAAGAGTTATACCAACGCAAGGGTTCCCAGGTGCCTACTTTCCGAGATGAAATAACTGCAGTTAAGTGGCTGCAAACATTTCTGAAGGAAGAAGGACAAAACCCCTTACTATTAGTCTTGGATGATGTTTGGTCTGGATCAGAATCCCTACTAGACAAGTTTCAGTTTGAAATGCCAGATTACAAGATTTTGGTCACATCAAGATCTGAATTTCCTAGATTTGGTTCCCCATATCATTTACGATCATTGGACTATGTCAATGCCATGAAACTTTTTCATCATTCAGCATCCTTGGGAGATAAAAGCTCACATATTCCAACAGACCTCGCGAGACGGATAGTAGAAGGCTGTAAGGGATTTCCACTTGCAATTACGGTGGCAGGAGGATCACTTTGCGGACAAAGTATAGAGATCTGGCAACAAAGACTAATTGAATGGTCCAAAGGGTCTTCTATTCTTGATTCTGAGACCGAATTGCTTCTTCGCCTCCAAAGCAGCTTAGTTGTCTCGGATAAAGAAACGGCTATCATCAGGGAGTGTTTCTTAGACCTTGGTTCATTTCCTGAAGACCAAAGAATCCCAGTTGCTGCCCTCATTGATATGTGGACAGAGTCATATGATCAGCTAGAAGAAGATACTTTGTGTGTTGCATACCTCCACAGGCTCACCAACCGAAGTCTGGCTAATCTTGTAGTCACACGGAAGGACCGAAGCCAGGGGGATGGATACTACAGCGAACATTTTGTTACTCAGCATGATCTGCTTAGAGAGTTGGCTATCTATGAGACAAGACAACAAGACCCAGGACATAGAGAAAGACTGATTATAAACATAACTGGAAACAATCTTCCAAAATGGTGGAGTGAACAGAAGTATGAATCCATGAAAGCACGCCTATTATCCATCTCAACTGATGGAACTTTCTCATCAAAATGGCACCACATGGAACTACCAGAAGCTGAGGTTCTAGTCTTGAATTTTCAGACGAAGAACTATGCCTTACCCAAATTTGTGGACACTATGCATAAATTGAAGGTACTTGTCGTCACAAATTCTGGTTTCTCACCTGCTGAACTAAGTAATATCCCACTACTGGTTTCGTTATCTCATCTAAAGAGAATTAGATTGGAGCGTGTCTCAATACCTTCAATAACCAAGAACCCCATACAATTGGTGGGTCTGAAGAAGATGTCTTTATTCATGTGTACCATTGGAAAAGCTTTCAGCGACAGTTCCATCCAGATTTCACACGCATTTCCAAATCTAGAGGAAATTAGCATTGACTATTGTAATGATTTGGTGGAAATTCCTGCCGACCTCTGTGATCTTATTCATCTAAGGAAGCTCAGTGTCACCAACTCACATAAGCTTCTTGCCCTGCCTGAAGAGATTGGAAAACTGGAAAATTTGGAACTGCTGAGACTGAGGTCATGTACAGACTTGGCACAATTGCCAGGCTCGAGCAGGAACCTAAAGAAGTTGATCTTTCTTGACATTTCTTATTGCTTTAGCATCAAGGAGTTGCCTGAAGATATTGGTGAAATGAGCAGCTTAAGAAAGCTCAACATGAGAGAATGCTCAAGATTGCAAGAAGTGCCTGTATCGGTTTTGGATCTTGAGCATTTGGAGGAAGTGATATGTGATGATGAGACAAGAAATTTATGGGAACCCTTACCTCTCGGAAACACAAAAATAGTGGTGGAAAAAGAAAATATCAACCTAAATTGGCTCCATAAGCTTCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

878

Amino Acids

99.32

Weight (kDa)

6.04

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 137 7.1e-24 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 233 - 366 4.8e-15 NB-ARC domain
AAA_22 PF13401 246 - 339 4e-06 AAA domain
LRR_14 PF23598 735 - 797 1.6e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1715
AarI CACCTGC 1 cut(s) 1963
Acc36I ACCTGC 1 cut(s) 1963
AccB1I GGYRCC 2 cut(s) 919, 1824
AccII CGCG 1 cut(s) 1217
AciI CCGC 4 cut(s) 507, 593, 604, 1284
AclWI GGATC 4 cut(s) 195, 1033, 1282, 2508
AcsI RAATTY 9 cut(s) 211, 827, 1097, 1864, 1895, 1939, 2193, 2293, 2545
AcuI CTGAAG 6 cut(s) 374, 992, 1473, 2090, 2292, 2436
AfaI GTAC 6 cut(s) 293, 351, 548, 1926, 2095, 2323
AfiI CCNNNNNNNGG 6 cut(s) 682, 907, 1109, 1567, 1603, 1981
AflII CTTAAG 2 cut(s) 728, 2440
AjnI CCWGG 4 cut(s) 915, 1602, 1690, 2341
AjuI GAANNNNNNNTTGG 4 cut(s) 371, 403, 770, 802
AloI GAACNNNNNNTCC 2 cut(s) 581, 613
Alw21I GWGCWC 2 cut(s) 81, 260
Alw26I GTCTC 7 cut(s) 1213, 1349, 1399, 1667, 2029, 2302, 2531
AlwI GGATC 4 cut(s) 195, 1033, 1282, 2508
AlwNI CAGNNNCTG 2 cut(s) 1475, 1847
Ama87I CYCGRG 1 cut(s) 2347
AoxI GGCC 1 cut(s) 559
ApeKI GCWGC 6 cut(s) 32, 326, 958, 1383, 1475, 2436
ApoI RAATTY 9 cut(s) 211, 827, 1097, 1864, 1895, 1939, 2193, 2293, 2545
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
Asp700I GAANNNNTTC 1 cut(s) 1365
AspLEI GCGC 3 cut(s) 133, 361, 502
AspS9I GGNCC 3 cut(s) 146, 1326, 1594
AsuC2I CCSGG 1 cut(s) 765
AsuHPI GGTGA 5 cut(s) 26, 1546, 1944, 2233, 2438
AvaI CYCGRG 1 cut(s) 2347
AvaII GGWCC 3 cut(s) 146, 1326, 1594
BanI GGYRCC 2 cut(s) 919, 1824
BanII GRGCYC 1 cut(s) 81
BbsI GAAGAC 4 cut(s) 389, 618, 1329, 1461
Bbv12I GWGCWC 2 cut(s) 81, 260
BbvCI CCTCAGC 1 cut(s) 1848
BbvI GCAGC 6 cut(s) 19, 313, 945, 1395, 1462, 2448
BccI CCATC 7 cut(s) 312, 368, 728, 1604, 1796, 1799, 2132
BceAI ACGGC 1 cut(s) 1425
BciT130I CCWGG 4 cut(s) 917, 1604, 1692, 2343
BciVI GTATCC 1 cut(s) 1607
BclI TGATCA 2 cut(s) 153, 1507
BcnI CCSGG 1 cut(s) 765
BcoDI GTCTC 7 cut(s) 1213, 1349, 1399, 1667, 2029, 2302, 2531
BfaI CTAG 8 cut(s) 134, 579, 749, 1040, 1104, 1514, 1856, 2153
BfmI CTRYAG 2 cut(s) 945, 1618
BfoI RGCGCY 1 cut(s) 503
BfrI CTTAAG 2 cut(s) 728, 2440
BfuAI ACCTGC 1 cut(s) 1963
BfuI GTATCC 1 cut(s) 1607
BglII AGATCT 2 cut(s) 1091, 1299
BisI GCNGC 6 cut(s) 33, 327, 959, 1384, 1476, 2437
BlpI GCTNAGC 1 cut(s) 503
BlsI GCNGC 6 cut(s) 34, 328, 960, 1385, 1477, 2438
Bme1390I CCNGG 5 cut(s) 765, 917, 1604, 1692, 2343
Bme18I GGWCC 3 cut(s) 146, 1326, 1594
BmeT110I CYCGRG 1 cut(s) 2347
BmgT120I GGNCC 3 cut(s) 146, 1326, 1594
BmiI GGNNCC 7 cut(s) 913, 921, 1114, 1826, 2357, 2556, 2618
BmrFI CCNGG 5 cut(s) 765, 917, 1604, 1692, 2343
BmrI ACTGGG 3 cut(s) 432, 670, 1463
BmsI GCATC 4 cut(s) 313, 735, 1184, 2409
BmuI ACTGGG 3 cut(s) 432, 670, 1463
BoxI GACNNNNGTC 1 cut(s) 2314
BpiI GAAGAC 4 cut(s) 389, 618, 1329, 1461
BplI GAGNNNNNCTC 2 cut(s) 2009, 2041
BpmI CTGGAG 1 cut(s) 57
Bpu10I CCTNAGC 2 cut(s) 875, 1848
Bpu1102I GCTNAGC 1 cut(s) 503
BpuEI CTTGAG 2 cut(s) 2452, 2525
BpuMI CCSGG 1 cut(s) 765
BsaBI GATNNNNATC 1 cut(s) 1030
BsaI GGTCTC 1 cut(s) 1349
BsaJI CCNNGG 6 cut(s) 387, 915, 1179, 1438, 1603, 1690
BsaXI ACNNNNNCTCC 9 cut(s) 33, 96, 126, 530, 560, 581, 611, 627, 657
Bsc4I CCNNNNNNNGG 6 cut(s) 682, 907, 1109, 1567, 1603, 1981
Bse1I ACTGG 9 cut(s) 149, 189, 427, 480, 676, 1469, 1729, 1986, 2292
Bse3DI GCAATG 1 cut(s) 181
Bse8I GATNNNNATC 1 cut(s) 1030
BseBI CCWGG 4 cut(s) 917, 1604, 1692, 2343
BseDI CCNNGG 6 cut(s) 387, 915, 1179, 1438, 1603, 1690
BseJI GATNNNNATC 1 cut(s) 1030
BseLI CCNNNNNNNGG 6 cut(s) 682, 907, 1109, 1567, 1603, 1981
BseMI GCAATG 1 cut(s) 181
BseMII CTCAG 8 cut(s) 517, 866, 1344, 1652, 1839, 2248, 2297, 2303
BseNI ACTGG 9 cut(s) 149, 189, 427, 480, 676, 1469, 1729, 1986, 2292
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 6 cut(s) 19, 313, 945, 1395, 1462, 2448
Bsh1236I CGCG 1 cut(s) 1217
BshFI GGCC 1 cut(s) 561
BshNI GGYRCC 2 cut(s) 919, 1824
BsiHKAI GWGCWC 2 cut(s) 81, 260
BsiHKCI CYCGRG 1 cut(s) 2347
BsiSI CCGG 1 cut(s) 765
BslI CCNNNNNNNGG 6 cut(s) 682, 907, 1109, 1567, 1603, 1981
BsmAI GTCTC 7 cut(s) 1213, 1349, 1399, 1667, 2029, 2302, 2531
BsmBI CGTCTC 1 cut(s) 1213
BsmI GAATGC 1 cut(s) 2468
BsnI GGCC 1 cut(s) 561
Bso31I GGTCTC 1 cut(s) 1349
BsoBI CYCGRG 1 cut(s) 2347
Bsp1286I GDGCHC 2 cut(s) 81, 260
Bsp1407I TGTACA 1 cut(s) 2321
Bsp1720I GCTNAGC 1 cut(s) 503
Bsp68I TCGCGA 1 cut(s) 1217
BspACI CCGC 4 cut(s) 507, 593, 604, 1284
BspANI GGCC 1 cut(s) 561
BspCNI CTCAG 8 cut(s) 516, 867, 1345, 1651, 1840, 2247, 2298, 2304
BspFNI CGCG 1 cut(s) 1217
BspLI GGNNCC 7 cut(s) 913, 921, 1114, 1826, 2357, 2556, 2618
BspMAI CTGCAG 1 cut(s) 949
BspMI ACCTGC 1 cut(s) 1963
BspPI GGATC 4 cut(s) 195, 1033, 1282, 2508
BspT107I GGYRCC 2 cut(s) 919, 1824
BspTI CTTAAG 2 cut(s) 728, 2440
BspTNI GGTCTC 1 cut(s) 1349
BsrDI GCAATG 1 cut(s) 181
BsrGI TGTACA 1 cut(s) 2321
BsrI ACTGG 9 cut(s) 149, 189, 427, 480, 676, 1469, 1729, 1986, 2292
BssECI CCNNGG 6 cut(s) 387, 915, 1179, 1438, 1603, 1690
BssT1I CCWWGG 3 cut(s) 387, 1179, 1438
Bst2UI CCWGG 4 cut(s) 917, 1604, 1692, 2343
Bst4CI ACNGT 5 cut(s) 342, 685, 856, 1264, 2120
Bst6I CTCTTC 1 cut(s) 2268
BstAFI CTTAAG 2 cut(s) 728, 2440
BstAPI GCANNNNNTGC 2 cut(s) 1648, 2482
BstAUI TGTACA 1 cut(s) 2321
BstC8I GCNNGC 2 cut(s) 172, 1780
BstFNI CGCG 1 cut(s) 1217
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 3 cut(s) 133, 361, 502
BstMAI GTCTC 7 cut(s) 1213, 1349, 1399, 1667, 2029, 2302, 2531
BstMWI GCNNNNNNNGC 5 cut(s) 76, 1380, 1648, 2482, 2623
BstNI CCWGG 4 cut(s) 917, 1604, 1692, 2343
BstNSI RCATGY 1 cut(s) 470
BstPAI GACNNNNGTC 1 cut(s) 2314
BstSCI CCNGG 5 cut(s) 763, 915, 1602, 1690, 2341
BstSFI CTRYAG 2 cut(s) 945, 1618
BstUI CGCG 1 cut(s) 1217
BstV1I GCAGC 6 cut(s) 19, 313, 945, 1395, 1462, 2448
BstV2I GAAGAC 4 cut(s) 389, 618, 1329, 1461
BstX2I RGATCY 4 cut(s) 187, 1091, 1299, 2500
BstXI CCANNNNNNTGG 4 cut(s) 1610, 1746, 1900, 2059
BstYI RGATCY 4 cut(s) 187, 1091, 1299, 2500
BsuI GTATCC 1 cut(s) 1607
BsuRI GGCC 1 cut(s) 561
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 5 cut(s) 156, 420, 756, 852, 2242
BtuMI TCGCGA 1 cut(s) 1217
BveI ACCTGC 1 cut(s) 1963
Cac8I GCNNGC 2 cut(s) 172, 1780
CaiI CAGNNNCTG 2 cut(s) 1475, 1847
CfoI GCGC 3 cut(s) 133, 361, 502
Cfr13I GGNCC 3 cut(s) 146, 1326, 1594
Csp6I GTAC 6 cut(s) 292, 350, 547, 1925, 2094, 2322
CspCI CAANNNNNGTGG 4 cut(s) 1245, 1280, 2565, 2600
CviAII CATG 8 cut(s) 467, 1153, 1643, 1771, 1831, 2089, 2319, 2455
CviQI GTAC 6 cut(s) 292, 350, 547, 1925, 2094, 2322
Eam1104I CTCTTC 1 cut(s) 2268
EarI CTCTTC 1 cut(s) 2268
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 3 cut(s) 387, 1179, 1438
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 1349
Eco47I GGWCC 3 cut(s) 146, 1326, 1594
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 6 cut(s) 374, 992, 1473, 2090, 2292, 2436
Eco88I CYCGRG 1 cut(s) 2347
EcoICRI GAGCTC 1 cut(s) 79
EcoRII CCWGG 4 cut(s) 915, 1602, 1690, 2341
EcoT14I CCWWGG 3 cut(s) 387, 1179, 1438
EcoT22I ATGCAT 1 cut(s) 1914
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 3 cut(s) 387, 1179, 1438
Esp3I CGTCTC 1 cut(s) 1213
FaeI CATG 8 cut(s) 470, 1156, 1646, 1774, 1834, 2092, 2322, 2458
FalI AAGNNNNNCTT 6 cut(s) 371, 403, 603, 635, 2357, 2389
FatI CATG 8 cut(s) 466, 1152, 1642, 1770, 1830, 2088, 2318, 2454
FauI CCCGC 1 cut(s) 586
FauNDI CATATG 2 cut(s) 225, 1504
FbaI TGATCA 2 cut(s) 153, 1507
Fnu4HI GCNGC 6 cut(s) 33, 327, 959, 1384, 1476, 2437
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 6 cut(s) 33, 327, 959, 1384, 1476, 2437
FspBI CTAG 8 cut(s) 134, 579, 749, 1040, 1104, 1514, 1856, 2153
GlaI GCGC 3 cut(s) 132, 360, 501
GluI GCNGC 6 cut(s) 33, 327, 959, 1384, 1476, 2437
GsuI CTGGAG 1 cut(s) 57
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 1 cut(s) 561
HapII CCGG 1 cut(s) 765
HhaI GCGC 3 cut(s) 133, 361, 502
Hin1II CATG 8 cut(s) 470, 1156, 1646, 1774, 1834, 2092, 2322, 2458
Hin6I GCGC 3 cut(s) 131, 359, 500
HinP1I GCGC 3 cut(s) 131, 359, 500
HindIII AAGCTT 3 cut(s) 2106, 2255, 2624
HinfI GANTC 5 cut(s) 1031, 1349, 1464, 1499, 1766
HpaII CCGG 1 cut(s) 765
HphI GGTGA 5 cut(s) 26, 1546, 1944, 2233, 2438
Hpy166II GTNNAC 5 cut(s) 681, 1494, 1754, 1903, 2094
Hpy188I TCNGA 9 cut(s) 932, 972, 1030, 1096, 1354, 1399, 1872, 2070, 2570
Hpy8I GTNNAC 5 cut(s) 681, 1494, 1754, 1903, 2094
HpyCH4III ACNGT 5 cut(s) 342, 685, 856, 1264, 2120
HpyCH4V TGCA 8 cut(s) 473, 889, 947, 961, 1256, 1538, 1912, 2476
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 1380, 1648, 2482, 2623
Hsp92II CATG 8 cut(s) 470, 1156, 1646, 1774, 1834, 2092, 2322, 2458
HspAI GCGC 3 cut(s) 131, 359, 500
Ksp22I TGATCA 2 cut(s) 153, 1507
LmnI GCTCC 5 cut(s) 635, 647, 766, 2017, 2622
Lsp1109I GCAGC 6 cut(s) 19, 313, 945, 1395, 1462, 2448
LweI GCATC 4 cut(s) 313, 735, 1184, 2409
MaeI CTAG 8 cut(s) 134, 579, 749, 1040, 1104, 1514, 1856, 2153
MaeIII GTNAC 8 cut(s) 418, 754, 850, 1081, 1582, 1633, 1933, 2239
MfeI CAATTG 2 cut(s) 2057, 2336
MflI RGATCY 4 cut(s) 187, 1091, 1299, 2500
MhlI GDGCHC 2 cut(s) 81, 260
MlyI GAGTC 1 cut(s) 1508
MmeI TCCRAC 4 cut(s) 324, 625, 667, 1229
Mph1103I ATGCAT 1 cut(s) 1914
MroXI GAANNNNTTC 1 cut(s) 1365
MseI TTAA 5 cut(s) 72, 621, 729, 951, 2441
MslI CAYNNNNRTG 2 cut(s) 1487, 1819
MspA1I CMGCKG 1 cut(s) 507
MspCI CTTAAG 2 cut(s) 728, 2440
MspI CCGG 1 cut(s) 765
MspR9I CCNGG 5 cut(s) 765, 917, 1604, 1692, 2343
MunI CAATTG 2 cut(s) 2057, 2336
Mva1269I GAATGC 1 cut(s) 2468
MvaI CCWGG 4 cut(s) 917, 1604, 1692, 2343
MvnI CGCG 1 cut(s) 1217
MwoI GCNNNNNNNGC 5 cut(s) 76, 1380, 1648, 2482, 2623
NciI CCSGG 1 cut(s) 765
NdeI CATATG 2 cut(s) 225, 1504
NlaIII CATG 8 cut(s) 470, 1156, 1646, 1774, 1834, 2092, 2322, 2458
NlaIV GGNNCC 7 cut(s) 913, 921, 1114, 1826, 2357, 2556, 2618
NmuCI GTSAC 7 cut(s) 418, 754, 850, 1081, 1582, 1933, 2239
NruI TCGCGA 1 cut(s) 1217
NsiI ATGCAT 1 cut(s) 1914
NspI RCATGY 1 cut(s) 470
PaeR7I CTCGAG 1 cut(s) 2347
PaqCI CACCTGC 1 cut(s) 1963
PcsI WCGNNNNNNNCGW 1 cut(s) 1594
PctI GAATGC 1 cut(s) 2468
PdmI GAANNNNTTC 1 cut(s) 1365
PfeI GAWTC 4 cut(s) 1031, 1349, 1464, 1766
PflFI GACNNNGTC 2 cut(s) 1142, 1498
PfoI TCCNGGA 1 cut(s) 763
PkrI GCNGC 6 cut(s) 34, 328, 960, 1385, 1477, 2438
PleI GAGTC 1 cut(s) 1507
PpsI GAGTC 1 cut(s) 1507
PshAI GACNNNNGTC 1 cut(s) 2314
PsiI TTATAA 1 cut(s) 1715
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 4 cut(s) 915, 1602, 1690, 2341
PspGI CCWGG 4 cut(s) 915, 1602, 1690, 2341
PspN4I GGNNCC 7 cut(s) 913, 921, 1114, 1826, 2357, 2556, 2618
PspPI GGNCC 3 cut(s) 146, 1326, 1594
PspXI VCTCGAGB 1 cut(s) 2347
PstI CTGCAG 1 cut(s) 949
PstNI CAGNNNCTG 2 cut(s) 1475, 1847
PsuI RGATCY 4 cut(s) 187, 1091, 1299, 2500
PsyI GACNNNGTC 2 cut(s) 1142, 1498
RruI TCGCGA 1 cut(s) 1217
RsaI GTAC 6 cut(s) 293, 351, 548, 1926, 2095, 2323
RsaNI GTAC 6 cut(s) 292, 350, 547, 1925, 2094, 2322
RseI CAYNNNNRTG 2 cut(s) 1487, 1819
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 5 cut(s) 72, 621, 729, 951, 2441
SatI GCNGC 6 cut(s) 33, 327, 959, 1384, 1476, 2437
Sau96I GGNCC 3 cut(s) 146, 1326, 1594
SchI GAGTC 1 cut(s) 1508
ScrFI CCNGG 5 cut(s) 765, 917, 1604, 1692, 2343
SduI GDGCHC 2 cut(s) 81, 260
SfaNI GCATC 4 cut(s) 313, 735, 1184, 2409
SfcI CTRYAG 2 cut(s) 945, 1618
Sfr274I CTCGAG 1 cut(s) 2347
SinI GGWCC 3 cut(s) 146, 1326, 1594
SlaI CTCGAG 1 cut(s) 2347
SmiMI CAYNNNNRTG 2 cut(s) 1487, 1819
SmlI CTYRAG 5 cut(s) 728, 2347, 2440, 2467, 2504
SmoI CTYRAG 5 cut(s) 728, 2347, 2440, 2467, 2504
SsiI CCGC 4 cut(s) 507, 593, 604, 1284
SspI AATATT 1 cut(s) 55
SspMI CTAG 8 cut(s) 134, 579, 749, 1040, 1104, 1514, 1856, 2153
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 5 cut(s) 763, 915, 1602, 1690, 2341
StyI CCWWGG 3 cut(s) 387, 1179, 1438
TaaI ACNGT 5 cut(s) 342, 685, 856, 1264, 2120
TaqI TCGA 2 cut(s) 743, 2348
TaqII GACCGA 3 cut(s) 630, 1373, 1611
TatI WGTACW 3 cut(s) 291, 349, 2321
TfiI GAWTC 4 cut(s) 1031, 1349, 1464, 1766
Tru1I TTAA 5 cut(s) 72, 621, 729, 951, 2441
Tru9I TTAA 5 cut(s) 72, 621, 729, 951, 2441
TscAI CASTG 5 cut(s) 156, 427, 763, 859, 2242
TseFI GTSAC 7 cut(s) 418, 754, 850, 1081, 1582, 1933, 2239
TseI GCWGC 6 cut(s) 32, 326, 958, 1383, 1475, 2436
Tsp45I GTSAC 7 cut(s) 418, 754, 850, 1081, 1582, 1933, 2239
TspDTI ATGAA 9 cut(s) 734, 951, 1154, 1169, 1434, 1779, 1787, 2077, 2210
TspGWI ACGGA 2 cut(s) 1237, 1603
TspRI CASTG 5 cut(s) 156, 427, 763, 859, 2242
Tth111I GACNNNGTC 2 cut(s) 1142, 1498
Vha464I CTTAAG 2 cut(s) 728, 2440
VpaK11BI GGWCC 3 cut(s) 146, 1326, 1594
XapI RAATTY 9 cut(s) 211, 827, 1097, 1864, 1895, 1939, 2193, 2293, 2545
XbaI TCTAGA 1 cut(s) 2152
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 4 cut(s) 487, 683, 1564, 2059
XhoI CTCGAG 1 cut(s) 2347
XmnI GAANNNNTTC 1 cut(s) 1365
XspI CTAG 8 cut(s) 134, 579, 749, 1040, 1104, 1514, 1856, 2153
Zsp2I ATGCAT 1 cut(s) 1914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.