Rh2CG039000

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000523
Physical Location & Seq
Forward (+)
3113412 .. 3116435
3024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG039000.1

Sequence Viewer

Length: 2118 bp
ATGTGCTCTATAAGGGCCTCTATGATCAACATCTACGCTATCGGGGTTTTACAAATTGGAAAAGTAGTTTGGAAAGTGAGAAGATCGATGAGGAATTCAGGTGGGTGGATTCTGGGGACGTTATTATTTTTGGGTGGTTTTTTCTTTGTTCATGTTGCGGCAAGTTCACCGAGAAGTCATCGACAACTGGCGCAAGATGCGGTCGAGATCAAGATGGTAAAAAATGGAGTTGTGTTGGATAATAAGCTTGTTCAACTGACTTTTTCTAATCCTGGTGGGGATGTTATTGGAATAAGGTATGCAGGAACTGACAACTTACTTGCAATTAACAACAAGGAAGGTGATAGAGGGTACTGGGATGTGGTTTGGAATTACCCGGGAGAGGCGAAAAACACACAATACAGACTACAAGGAACACAATTTAAAGTAATCACGGAGAGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAATGCCAACTCCGGTGTGACAGCTCCGGTGAATGTTGACAGAAGATACATAATGCAGCAAGGACGTTCCGGGTTCTATACCTATGCCATCTTCGAGCGCCTTGAAGGGTGGCCAGGAGGGGACATGGAGACAGTAAGAGTGGCTTACAAGCTTCATCAAGCCAAGTTTCGATATATGGCGCTATCGGACACTAGGCAAAGGTTCATGCCAACAGCCGATGACCGAGCACGCGGTAGGCCACTTGCATTCAAAGAAGCTGTTCTACTGAACAATGAGACGAGCAATCCTGAACTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCTCCACCGGTGGGATTTTGGATGATCACACCTAGTGATGAGTTTCGTATAGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTCCCTAACAGTGTTTGCTGCTCATCATTATGTTGGTAAAGATGTTGGGCTGACATTCCAAGAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTTTATCTATCTTAACTCGGCAGCACGAAACACAGCTGATAGTCCTTCAACGATCCGTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCATACAATTTCATTAGTTCTCCCGACTTTCCTTCTTCGGATCAACGTGGATCAGTTTCCGGCCAGTTACTAGTACATGATCGATACATTGAAAGTCTTCCAAGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAGGGAAAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTCATTGAAAATATTAGACCCGGTAAATATAATTTGTATGCATCTGTTCCAGGGATAGTTGGAGACTACAAATATGAGGTTGACATTACAATTGAACCAGGAAGTAAAATCCAATTGGCTAGTATTACTTACAAACCTCCGAGAACCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCAGAGTTCTACATACCGGATCCATATCCAACTCTCACGAACAAGTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAGTACGGGTTGTGGGCACGTTATTATGAGCGTTACCCTAACAATGACCTCATCTACCGTGTTGGTGCTAACAATTACAGTGAGGATTGGTTTTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGCATGTAGGAACTACATGGCAGATACAGTTTGAACTCAAGAATGTGGTCAATCCCGGAAACTATACACTTCAACTGGCCTTGGCATCAGCCACTTATGCAGAATTGCAAGTTCGGGTTAACAATTTGAATGCCAAGCCACCTCTTTTTTCGACAGGGCTAATAGGCGACGACAATGCTATAGCAAGACTTGGAATTCATGGCTTATACAGGTTATGGAGTATTGATGTACCAAGCACTCTACTACGCGAAGGAAGCAACACCATCTATCTTACTCAGTCCAGAGATGGCAATTCACCTTTCCAAGGAGTCATGTATGATTATATAAGACTAGAAGGACCTTCTGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

705

Amino Acids

79.72

Weight (kDa)

8.15

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 62 - 261 2.9e-71 Rhamnogalacturonate lyase family
fn3_3 PF14686 424 - 495 2.4e-24 Polysaccharide lyase family 4, domain II
CBM-like PF14683 508 - 698 4e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 708, 2010
AciI CCGC 3 cut(s) 158, 200, 708
AclWI GGATC 6 cut(s) 1093, 1203, 1213, 1312, 1571, 1584
AcoI YGGCCR 5 cut(s) 587, 804, 1154, 1216, 1759
AcsI RAATTY 2 cut(s) 94, 1956
AdeI CACNNNGTG 1 cut(s) 890
AfaI GTAC 7 cut(s) 353, 800, 1105, 1230, 1607, 1640, 1992
AfiI CCNNNNNNNGG 4 cut(s) 382, 866, 1514, 2066
AgeI ACCGGT 2 cut(s) 862, 1514
AhlI ACTAGT 1 cut(s) 1225
AjnI CCWGG 4 cut(s) 271, 589, 1417, 1465
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 6 cut(s) 247, 500, 628, 734, 1082, 1556
AluI AGCT 6 cut(s) 247, 500, 628, 734, 1082, 1556
Alw21I GWGCWC 2 cut(s) 8, 706
Alw26I GTCTC 3 cut(s) 599, 746, 1425
AlwI GGATC 6 cut(s) 1093, 1203, 1213, 1312, 1571, 1584
AlwNI CAGNNNCTG 1 cut(s) 308
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 9 cut(s) 15, 440, 587, 713, 804, 1154, 1216, 1759, 1839
ApeKI GCWGC 5 cut(s) 532, 968, 1067, 1286, 1556
ApoI RAATTY 2 cut(s) 94, 1956
AsiGI ACCGGT 2 cut(s) 862, 1514
Asp700I GAANNNNTTC 2 cut(s) 735, 1251
AspLEI GCGC 3 cut(s) 193, 576, 658
AspS9I GGNCC 7 cut(s) 15, 911, 925, 944, 1040, 1517, 2099
AsuC2I CCSGG 6 cut(s) 377, 378, 547, 1293, 1389, 1818
AsuHPI GGTGA 7 cut(s) 159, 353, 460, 517, 922, 1028, 2049
AvaI CYCGRG 1 cut(s) 376
AvaII GGWCC 6 cut(s) 911, 925, 944, 1040, 1517, 2099
BaeGI GKGCMC 1 cut(s) 1654
BalI TGGCCA 2 cut(s) 589, 1156
BamHI GGATCC 1 cut(s) 1576
BarI GAAGNNNNNNTAC 2 cut(s) 723, 755
BbsI GAAGAC 1 cut(s) 1244
Bbv12I GWGCWC 2 cut(s) 8, 706
BbvI GCAGC 5 cut(s) 544, 955, 1079, 1273, 1543
BccI CCATC 4 cut(s) 208, 572, 2033, 2042
BcgI CGANNNNNNTGC 2 cut(s) 1919, 1953
BciT130I CCWGG 4 cut(s) 273, 591, 1419, 1467
BciVI GTATCC 1 cut(s) 1322
BclI TGATCA 2 cut(s) 24, 879
BcnI CCSGG 6 cut(s) 377, 378, 547, 1293, 1389, 1818
BcoDI GTCTC 3 cut(s) 599, 746, 1425
BcuI ACTAGT 1 cut(s) 1225
BfaI CTAG 6 cut(s) 669, 888, 1226, 1488, 1743, 2093
BfmI CTRYAG 2 cut(s) 1557, 1941
BfoI RGCGCY 2 cut(s) 577, 659
BfuI GTATCC 1 cut(s) 1322
BisI GCNGC 6 cut(s) 159, 533, 969, 1068, 1287, 1557
BlsI GCNGC 6 cut(s) 160, 534, 970, 1069, 1288, 1558
BmcAI AGTACT 1 cut(s) 800
Bme18I GGWCC 6 cut(s) 911, 925, 944, 1040, 1517, 2099
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 7 cut(s) 15, 911, 925, 944, 1040, 1517, 2099
BmiI GGNNCC 1 cut(s) 1578
BmrI ACTGGG 1 cut(s) 364
BmsI GCATC 3 cut(s) 187, 1418, 1856
BmuI ACTGGG 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 1244
BpuEI CTTGAG 2 cut(s) 1719, 1784
BpuMI CCSGG 6 cut(s) 377, 378, 547, 1293, 1389, 1818
Bsa29I ATCGAT 2 cut(s) 86, 1237
BsaAI YACGTR 1 cut(s) 1276
BsaBI GATNNNNATC 3 cut(s) 29, 792, 1581
BsaJI CCNNGG 6 cut(s) 376, 807, 1256, 1418, 1842, 2065
BsaWI WCCGGW 5 cut(s) 488, 502, 862, 1514, 1573
Bsc4I CCNNNNNNNGG 4 cut(s) 382, 866, 1514, 2066
Bse118I RCCGGY 3 cut(s) 862, 1514, 1761
Bse1I ACTGG 4 cut(s) 192, 359, 1219, 1842
Bse8I GATNNNNATC 3 cut(s) 29, 792, 1581
BseBI CCWGG 4 cut(s) 273, 591, 1419, 1467
BseCI ATCGAT 2 cut(s) 86, 1237
BseDI CCNNGG 6 cut(s) 376, 807, 1256, 1418, 1842, 2065
BseGI GGATG 4 cut(s) 286, 364, 790, 882
BseJI GATNNNNATC 3 cut(s) 29, 792, 1581
BseLI CCNNNNNNNGG 4 cut(s) 382, 866, 1514, 2066
BseMII CTCAG 2 cut(s) 2051, 2097
BseNI ACTGG 4 cut(s) 192, 359, 1219, 1842
BseSI GKGCMC 1 cut(s) 1654
BseX3I CGGCCG 1 cut(s) 804
BseXI GCAGC 5 cut(s) 544, 955, 1079, 1273, 1543
BsgI GTGCAG 1 cut(s) 1272
Bsh1236I CGCG 2 cut(s) 708, 2010
Bsh1285I CGRYCG 2 cut(s) 204, 807
BshFI GGCC 9 cut(s) 17, 442, 589, 715, 806, 1156, 1218, 1761, 1841
BshTI ACCGGT 2 cut(s) 862, 1514
BshVI ATCGAT 2 cut(s) 86, 1237
BsiEI CGRYCG 2 cut(s) 204, 807
BsiHKAI GWGCWC 2 cut(s) 8, 706
BsiHKCI CYCGRG 1 cut(s) 376
BslFI GGGAC 2 cut(s) 130, 611
BslI CCNNNNNNNGG 4 cut(s) 382, 866, 1514, 2066
BsmAI GTCTC 3 cut(s) 599, 746, 1425
BsmBI CGTCTC 1 cut(s) 746
BsmFI GGGAC 2 cut(s) 130, 611
BsmI GAATGC 3 cut(s) 722, 1539, 1897
BsnI GGCC 9 cut(s) 17, 442, 589, 715, 806, 1156, 1218, 1761, 1841
BsoBI CYCGRG 1 cut(s) 376
Bsp1286I GDGCHC 3 cut(s) 8, 706, 1654
Bsp1407I TGTACA 1 cut(s) 1605
BspACI CCGC 3 cut(s) 158, 200, 708
BspANI GGCC 9 cut(s) 17, 442, 589, 715, 806, 1156, 1218, 1761, 1841
BspCNI CTCAG 2 cut(s) 2050, 2098
BspDI ATCGAT 2 cut(s) 86, 1237
BspFNI CGCG 2 cut(s) 708, 2010
BspLI GGNNCC 1 cut(s) 1578
BspMAI CTGCAG 1 cut(s) 1561
BspPI GGATC 6 cut(s) 1093, 1203, 1213, 1312, 1571, 1584
BsrFI RCCGGY 3 cut(s) 862, 1514, 1761
BsrGI TGTACA 1 cut(s) 1605
BsrI ACTGG 4 cut(s) 192, 359, 1219, 1842
BssAI RCCGGY 3 cut(s) 862, 1514, 1761
BssECI CCNNGG 6 cut(s) 376, 807, 1256, 1418, 1842, 2065
BssT1I CCWWGG 3 cut(s) 1256, 1842, 2065
Bst2UI CCWGG 4 cut(s) 273, 591, 1419, 1467
Bst4CI ACNGT 5 cut(s) 610, 961, 1694, 1715, 1791
BstAUI TGTACA 1 cut(s) 1605
BstBAI YACGTR 1 cut(s) 1276
BstC8I GCNNGC 2 cut(s) 706, 1763
BstDEI CTNAG 2 cut(s) 2037, 2106
BstENI CCTNNNNNAGG 1 cut(s) 2064
BstF5I GGATG 4 cut(s) 286, 364, 790, 882
BstFNI CGCG 2 cut(s) 708, 2010
BstH2I RGCGCY 2 cut(s) 577, 659
BstHHI GCGC 3 cut(s) 193, 576, 658
BstMAI GTCTC 3 cut(s) 599, 746, 1425
BstMCI CGRYCG 2 cut(s) 204, 807
BstMWI GCNNNNNNNGC 3 cut(s) 197, 1859, 2016
BstNI CCWGG 4 cut(s) 273, 591, 1419, 1467
BstNSI RCATGY 1 cut(s) 1769
BstSFI CTRYAG 2 cut(s) 1557, 1941
BstSLI GKGCMC 1 cut(s) 1654
BstSNI TACGTA 1 cut(s) 1276
BstUI CGCG 2 cut(s) 708, 2010
BstV1I GCAGC 5 cut(s) 544, 955, 1079, 1273, 1543
BstV2I GAAGAC 1 cut(s) 1244
BstX2I RGATCY 1 cut(s) 1576
BstYI RGATCY 1 cut(s) 1576
BstZI CGGCCG 1 cut(s) 804
Bsu15I ATCGAT 2 cut(s) 86, 1237
BsuI GTATCC 1 cut(s) 1322
BsuRI GGCC 9 cut(s) 17, 442, 589, 715, 806, 1156, 1218, 1761, 1841
BsuTUI ATCGAT 2 cut(s) 86, 1237
BtsCI GGATG 4 cut(s) 286, 364, 790, 882
BtsIMutI CAGTG 2 cut(s) 966, 1720
Cac8I GCNNGC 2 cut(s) 706, 1763
CaiI CAGNNNCTG 1 cut(s) 308
CfoI GCGC 3 cut(s) 193, 576, 658
Cfr10I RCCGGY 3 cut(s) 862, 1514, 1761
Cfr13I GGNCC 7 cut(s) 15, 911, 925, 944, 1040, 1517, 2099
Cfr9I CCCGGG 1 cut(s) 376
ClaI ATCGAT 2 cut(s) 86, 1237
Csp6I GTAC 7 cut(s) 352, 799, 1104, 1229, 1606, 1639, 1991
CspAI ACCGGT 2 cut(s) 862, 1514
CviQI GTAC 7 cut(s) 352, 799, 1104, 1229, 1606, 1639, 1991
DdeI CTNAG 2 cut(s) 2037, 2106
DraI TTTAAA 1 cut(s) 424
DraIII CACNNNGTG 1 cut(s) 890
EaeI YGGCCR 5 cut(s) 587, 804, 1154, 1216, 1759
EagI CGGCCG 1 cut(s) 804
EclXI CGGCCG 1 cut(s) 804
Eco105I TACGTA 1 cut(s) 1276
Eco130I CCWWGG 3 cut(s) 1256, 1842, 2065
Eco32I GATATC 1 cut(s) 820
Eco47I GGWCC 6 cut(s) 911, 925, 944, 1040, 1517, 2099
Eco52I CGGCCG 1 cut(s) 804
Eco88I CYCGRG 1 cut(s) 376
EcoNI CCTNNNNNAGG 1 cut(s) 2064
EcoO109I RGGNCCY 3 cut(s) 15, 925, 2099
EcoRI GAATTC 2 cut(s) 94, 1956
EcoRII CCWGG 4 cut(s) 271, 589, 1417, 1465
EcoRV GATATC 1 cut(s) 820
EcoT14I CCWWGG 3 cut(s) 1256, 1842, 2065
EcoT22I ATGCAT 1 cut(s) 1411
ErhI CCWWGG 3 cut(s) 1256, 1842, 2065
Esp3I CGTCTC 1 cut(s) 746
FalI AAGNNNNNCTT 4 cut(s) 604, 636, 1255, 1287
FaqI GGGAC 2 cut(s) 130, 611
FbaI TGATCA 2 cut(s) 24, 879
Fnu4HI GCNGC 6 cut(s) 159, 533, 969, 1068, 1287, 1557
FokI GGATG 4 cut(s) 293, 371, 797, 889
Fsp4HI GCNGC 6 cut(s) 159, 533, 969, 1068, 1287, 1557
FspBI CTAG 6 cut(s) 669, 888, 1226, 1488, 1743, 2093
GlaI GCGC 3 cut(s) 192, 575, 657
GluI GCNGC 6 cut(s) 159, 533, 969, 1068, 1287, 1557
HaeII RGCGCY 2 cut(s) 577, 659
HaeIII GGCC 9 cut(s) 17, 442, 589, 715, 806, 1156, 1218, 1761, 1841
HhaI GCGC 3 cut(s) 193, 576, 658
Hin6I GCGC 3 cut(s) 191, 574, 656
HinP1I GCGC 3 cut(s) 191, 574, 656
HincII GTYRAC 3 cut(s) 514, 1450, 1882
HindII GTYRAC 3 cut(s) 514, 1450, 1882
HindIII AAGCTT 2 cut(s) 245, 626
HinfI GANTC 4 cut(s) 109, 1342, 1615, 2070
HpaI GTTAAC 1 cut(s) 1882
HphI GGTGA 7 cut(s) 159, 353, 460, 517, 922, 1028, 2049
Hpy166II GTNNAC 5 cut(s) 167, 514, 1106, 1450, 1882
Hpy188I TCNGA 5 cut(s) 664, 1040, 1195, 1509, 2107
Hpy188III TCNNGA 8 cut(s) 205, 211, 764, 1178, 1544, 1594, 1801, 2043
Hpy8I GTNNAC 5 cut(s) 167, 514, 1106, 1450, 1882
Hpy99I CGWCG 1 cut(s) 1934
HpyAV CCTTC 8 cut(s) 332, 575, 767, 1101, 1197, 2006, 2090, 2112
HpyCH4III ACNGT 5 cut(s) 610, 961, 1694, 1715, 1791
HpyCH4IV ACGT 6 cut(s) 119, 541, 939, 1201, 1275, 1654
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 1859, 2016
HpyF3I CTNAG 2 cut(s) 2037, 2106
HpySE526I ACGT 6 cut(s) 119, 541, 939, 1201, 1275, 1654
HspAI GCGC 3 cut(s) 191, 574, 656
KroI GCCGGC 1 cut(s) 1761
KroNI GCCGGC 1 cut(s) 1763
Ksp22I TGATCA 2 cut(s) 24, 879
KspAI GTTAAC 1 cut(s) 1882
LmnI GCTCC 1 cut(s) 505
Lsp1109I GCAGC 5 cut(s) 544, 955, 1079, 1273, 1543
LweI GCATC 3 cut(s) 187, 1418, 1856
MaeI CTAG 6 cut(s) 669, 888, 1226, 1488, 1743, 2093
MaeII ACGT 6 cut(s) 119, 541, 939, 1201, 1275, 1654
MaeIII GTNAC 4 cut(s) 493, 1221, 1667, 1738
MboII GAAGA 6 cut(s) 93, 531, 559, 1151, 1182, 1244
MfeI CAATTG 2 cut(s) 1458, 1481
MflI RGATCY 1 cut(s) 1576
MhlI GDGCHC 3 cut(s) 8, 706, 1654
MlsI TGGCCA 2 cut(s) 589, 1156
MluNI TGGCCA 2 cut(s) 589, 1156
MlyI GAGTC 3 cut(s) 1336, 1624, 2079
MmeI TCCRAC 7 cut(s) 216, 815, 922, 1282, 1408, 1602, 1610
Mox20I TGGCCA 2 cut(s) 589, 1156
Mph1103I ATGCAT 1 cut(s) 1411
MroNI GCCGGC 1 cut(s) 1761
MroXI GAANNNNTTC 2 cut(s) 735, 1251
MscI TGGCCA 2 cut(s) 589, 1156
MseI TTAA 4 cut(s) 327, 423, 1059, 1881
MslI CAYNNNNRTG 1 cut(s) 978
Msp20I TGGCCA 2 cut(s) 589, 1156
MspA1I CMGCKG 2 cut(s) 1082, 1556
MunI CAATTG 2 cut(s) 1458, 1481
Mva1269I GAATGC 3 cut(s) 722, 1539, 1897
MvaI CCWGG 4 cut(s) 273, 591, 1419, 1467
MvnI CGCG 2 cut(s) 708, 2010
MwoI GCNNNNNNNGC 3 cut(s) 197, 1859, 2016
NaeI GCCGGC 1 cut(s) 1763
NciI CCSGG 6 cut(s) 377, 378, 547, 1293, 1389, 1818
NgoMIV GCCGGC 1 cut(s) 1761
NlaIV GGNNCC 1 cut(s) 1578
NmeAIII GCCGAG 3 cut(s) 782, 832, 1043
NmuCI GTSAC 2 cut(s) 493, 1738
NsiI ATGCAT 1 cut(s) 1411
NspI RCATGY 1 cut(s) 1769
PctI GAATGC 3 cut(s) 722, 1539, 1897
PdiI GCCGGC 1 cut(s) 1763
PdmI GAANNNNTTC 2 cut(s) 735, 1251
PfeI GAWTC 1 cut(s) 109
PfoI TCCNGGA 1 cut(s) 1816
PinAI ACCGGT 2 cut(s) 862, 1514
PkrI GCNGC 6 cut(s) 160, 534, 970, 1069, 1288, 1558
PleI GAGTC 3 cut(s) 1336, 1623, 2078
PpsI GAGTC 3 cut(s) 1336, 1623, 2078
Ppu21I YACGTR 1 cut(s) 1276
PpuMI RGGWCCY 2 cut(s) 925, 2099
Psp5II RGGWCCY 2 cut(s) 925, 2099
Psp6I CCWGG 4 cut(s) 271, 589, 1417, 1465
PspGI CCWGG 4 cut(s) 271, 589, 1417, 1465
PspN4I GGNNCC 1 cut(s) 1578
PspPI GGNCC 7 cut(s) 15, 911, 925, 944, 1040, 1517, 2099
PspPPI RGGWCCY 2 cut(s) 925, 2099
PsrI GAACNNNNNNTAC 4 cut(s) 1504, 1536, 1589, 1621
PstI CTGCAG 1 cut(s) 1561
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 1 cut(s) 1576
PvuII CAGCTG 2 cut(s) 1082, 1556
RsaI GTAC 7 cut(s) 353, 800, 1105, 1230, 1607, 1640, 1992
RsaNI GTAC 7 cut(s) 352, 799, 1104, 1229, 1606, 1639, 1991
RseI CAYNNNNRTG 1 cut(s) 978
SaqAI TTAA 4 cut(s) 327, 423, 1059, 1881
SatI GCNGC 6 cut(s) 159, 533, 969, 1068, 1287, 1557
Sau96I GGNCC 7 cut(s) 15, 911, 925, 944, 1040, 1517, 2099
ScaI AGTACT 1 cut(s) 800
SchI GAGTC 3 cut(s) 1336, 1624, 2079
SduI GDGCHC 3 cut(s) 8, 706, 1654
SfaNI GCATC 3 cut(s) 187, 1418, 1856
SfcI CTRYAG 2 cut(s) 1557, 1941
SgrAI CRCCGGYG 1 cut(s) 862
SinI GGWCC 6 cut(s) 911, 925, 944, 1040, 1517, 2099
SmaI CCCGGG 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 978
SmlI CTYRAG 2 cut(s) 1734, 1799
SmoI CTYRAG 2 cut(s) 1734, 1799
SnaBI TACGTA 1 cut(s) 1276
SpeI ACTAGT 1 cut(s) 1225
SsiI CCGC 3 cut(s) 158, 200, 708
SspI AATATT 1 cut(s) 1381
SspMI CTAG 6 cut(s) 669, 888, 1226, 1488, 1743, 2093
StyI CCWWGG 3 cut(s) 1256, 1842, 2065
TaaI ACNGT 5 cut(s) 610, 961, 1694, 1715, 1791
TaiI ACGT 6 cut(s) 122, 544, 942, 1204, 1278, 1657
TaqI TCGA 7 cut(s) 86, 181, 204, 570, 646, 1237, 1913
TaqII GACCGA 1 cut(s) 714
TatI WGTACW 3 cut(s) 798, 1228, 1605
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 4 cut(s) 327, 423, 1059, 1881
Tru9I TTAA 4 cut(s) 327, 423, 1059, 1881
TscAI CASTG 2 cut(s) 966, 1720
TseFI GTSAC 2 cut(s) 493, 1738
TseI GCWGC 5 cut(s) 532, 968, 1067, 1286, 1556
Tsp45I GTSAC 2 cut(s) 493, 1738
TspDTI ATGAA 7 cut(s) 140, 454, 620, 670, 1156, 1368, 1949
TspGWI ACGGA 2 cut(s) 449, 1091
TspMI CCCGGG 1 cut(s) 376
TspRI CASTG 2 cut(s) 966, 1720
VpaK11BI GGWCC 6 cut(s) 911, 925, 944, 1040, 1517, 2099
XagI CCTNNNNNAGG 1 cut(s) 2064
XapI RAATTY 2 cut(s) 94, 1956
XceI RCATGY 1 cut(s) 1769
XmaI CCCGGG 1 cut(s) 376
XmnI GAANNNNTTC 2 cut(s) 735, 1251
XspI CTAG 6 cut(s) 669, 888, 1226, 1488, 1743, 2093
ZrmI AGTACT 1 cut(s) 800
Zsp2I ATGCAT 1 cut(s) 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.