MD12G1257400.v1.1

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000523
Physical Location & Seq
Reverse (-)
32471874 .. 32473922
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1257400.v1.1.491

Sequence Viewer

Length: 2049 bp
ATGCTTTCCATGGAGAAGGTGAGGCAATGGAGGAGATGGGGGATCCTATCATCGGTCGCACTACTTGGTTTGCTCTTCTTCTTCCCTACTAATAATGGTGCTTCCAAGACTACGACGATTTCTGGAAGGCGAGCACAGAGCAGCGTTATTAGTAATGAGCTCAACTTCAACAAGATGTTAATCCATCAAACCCAGAACACGGTGGAGCTGAACAATGGCATTGTCCGAGTCAAATTTTCAAATCCCGGAGGAGACATCATTGGAATTCAATACAAGGGAATTGACAACGTGCTCGAAACCAAAAACCACCCAAGCAATAGAGGTTACTGGGATCTTGTATGGGAAAATGGCTTGGACAGACTACAAGGAACAACATTTAAGATTGTTACATCGACGGCAGACCAAATAGAGATTTCTTTTAACAAAACTTATGATGTTTCCCTCGGTGATCGTCCCCTACCCTTAAATGTTGACAAAAGGTACATAATGCAGCGTGGTCGCGCTGGGTTCTACGCGTATGCCATATACGAGCGTCTACAAGGGTGGCCGGCATTGCGCATAGATCAAACAAGACTTGCTTTGAAACTTCGACAAGATAAATTTCAGTTCATGGCTGTATCAGACGACAGACAGAGATTCATGCCAACAACCGAAGATCGCAGCGGAGACCACGCTCAGACCCTTGCCTATCGTGAAGCTGTTCTTTTAACTAATCCAAGCAATCCTGAGTTTAAAGAAGAGGTGGATGACAAATACCAATACTCCAGTGACGTCAAAGACATTAAGGTTCATGGTTGGATCAGCACTGACGATGCTATAGGGCTTTGGATGATCACACCAGGTACCGAGTTTCGTTCAGCGGGGCCGTTCAAGCAGGAACTTACCTCTCATGTCGGCCCAACTGCCCTCACTGTGTTTCTTAGTGGTCACTACGTCGGGAAAGATGTTGGGTTGACCTTTGCAGATGGCGAGGCATGGAAGAAGGTTTTTGGGCCCGTCTTTGTGTATCTTAACTCAGACGTTCCAAGCTCCAATGCGGCCTCCTCTCTCTGGGAAAATGCAAAAGAAGAGATGTATGAAGAAGTAAAGAGTTGGCCATACAATTTCACCCAGTCCGAAGACTATCCTAGCTCCGGTGAACGAGGATCAGTTGCTGGTCTGTTACTAATACGGGATTCATACATCAACGAGAGCCTAGTTTCAGCAAGTTATGCTTATGTGGGATTGGCCGCAAGCGGAGACGCGGGATCATGGCAAACGGAATGCAAGGGTTATCAGTTCTGGACTCAAGCTGACAATCAAGGTTATTTCTACATGAAGGATGTTCGACCGGGGAACTATAGCTTGTATGCAACTGTTCCCGGCTTCATTGGGGACTACAAGTACGAGTATGATATTACAATTGAACCAGCAAGTGAAATTAAGTTAACTAATCTTACCTTTGAACCTCCGAGAAATGGTCCAACCTTGTGGGAGATCGGCATCCCGGATCGGTCAGCCGTAGAGTTCAACATACCGGATCCATATCCAAGCCTTGTGAACCGTTTGTTTACCAACAATTCCGCAAACAAGTTTAGGCAATATGGCTTGTGGGAACGATACGAAGATTACTACCCTGACCATGATCTCATCTACACCATCGGTACCAACAATTACAGTGATGATTGGTTTTATGCTCATGTGACAAGGAACATAAATGGTACATATGAGGCAACAACGTGGCAAATTGTATTTCAACTTGAAAACGTTTGGAGCACCGGAAATTATACGCTCCAATTGGCGCTGGCCTCGGCCACCTATGCCGAACTGCAAGTTCGATTCAACAACCAGAACGACACACCTCATTTTTCGACAGGGCTAATAGGGAGGGACAATGCCATAGCAAGGCATGGAATTCATGGTTTGTACTGGCTCTTCAGTGTTGATGTGCCGAGCTATCGACTACAAGAAGGATACAACACCATCTATCTTACACAATCAAGAACCTGGGGACCTTTTGCAGGGCTTATGTACGACTACATCCGACTAGAAGGACCTCCTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

683

Amino Acids

77.77

Weight (kDa)

5.35

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 58 - 248 2.5e-77 Rhamnogalacturonate lyase family
fn3_3 PF14686 404 - 475 6.7e-22 Polysaccharide lyase family 4, domain II
CBM-like PF14683 489 - 676 1e-47 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 774
Acc16I TGCGCA 1 cut(s) 557
Acc65I GGTACC 2 cut(s) 842, 1643
AccB1I GGYRCC 2 cut(s) 842, 1643
AccI GTMKAC 1 cut(s) 535
AccII CGCG 3 cut(s) 501, 515, 1244
AciI CCGC 7 cut(s) 663, 860, 1037, 1230, 1236, 1244, 1563
AclI AACGTT 1 cut(s) 1746
AclWI GGATC 9 cut(s) 37, 50, 339, 806, 1153, 1255, 1497, 1514, 1527
AcoI YGGCCR 4 cut(s) 545, 1094, 1227, 1791
AcsI RAATTY 4 cut(s) 233, 264, 599, 1893
AcuI CTGAAG 1 cut(s) 1900
AcyI GRCGYC 1 cut(s) 771
AfaI GTAC 7 cut(s) 482, 844, 1385, 1645, 1702, 1907, 2012
AfiI CCNNNNNNNGG 7 cut(s) 52, 199, 1050, 1133, 1457, 1884, 2000
AflIII ACRYGT 1 cut(s) 513
AjnI CCWGG 2 cut(s) 838, 1985
AluBI AGCT 8 cut(s) 160, 208, 698, 1029, 1131, 1292, 1344, 1935
AluI AGCT 8 cut(s) 160, 208, 698, 1029, 1131, 1292, 1344, 1935
Alw21I GWGCWC 4 cut(s) 136, 162, 294, 1757
Alw26I GTCTC 3 cut(s) 246, 660, 1233
AlwI GGATC 9 cut(s) 37, 50, 339, 806, 1153, 1255, 1497, 1514, 1527
AlwNI CAGNNNCTG 1 cut(s) 1154
AoxI GGCC 9 cut(s) 545, 863, 895, 992, 1038, 1094, 1227, 1785, 1791
ApaI GGGCCC 1 cut(s) 996
ApeKI GCWGC 3 cut(s) 141, 490, 660
ApoI RAATTY 4 cut(s) 233, 264, 599, 1893
Asp700I GAANNNNTTC 1 cut(s) 699
Asp718I GGTACC 2 cut(s) 842, 1643
AspLEI GCGC 3 cut(s) 503, 558, 1783
AspS9I GGNCC 7 cut(s) 863, 896, 992, 993, 1460, 1991, 2033
AsuC2I CCSGG 4 cut(s) 246, 1332, 1362, 1487
AsuHPI GGTGA 4 cut(s) 31, 458, 1099, 1148
AvaII GGWCC 3 cut(s) 1460, 1991, 2033
BaeGI GKGCMC 1 cut(s) 996
BalI TGGCCA 1 cut(s) 1096
BamHI GGATCC 2 cut(s) 42, 1519
BanI GGYRCC 2 cut(s) 842, 1643
BanII GRGCYC 2 cut(s) 162, 996
BarI GAAGNNNNNNTAC 2 cut(s) 1898, 1930
BbsI GAAGAC 1 cut(s) 1125
Bbv12I GWGCWC 4 cut(s) 136, 162, 294, 1757
BbvI GCAGC 3 cut(s) 153, 502, 672
BccI CCATC 5 cut(s) 30, 192, 959, 1646, 1970
BceAI ACGGC 3 cut(s) 411, 850, 1484
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciT130I CCWGG 2 cut(s) 840, 1987
BciVI GTATCC 1 cut(s) 1946
BclI TGATCA 1 cut(s) 831
BcnI CCSGG 4 cut(s) 246, 1332, 1362, 1487
BcoDI GTCTC 3 cut(s) 246, 660, 1233
BfaI CTAG 3 cut(s) 1128, 1196, 2027
BfmI CTRYAG 2 cut(s) 816, 1339
BfoI RGCGCY 1 cut(s) 1784
BfuI GTATCC 1 cut(s) 1946
BisI GCNGC 5 cut(s) 142, 491, 661, 1038, 1230
BlsI GCNGC 5 cut(s) 143, 492, 662, 1039, 1231
Bme1390I CCNGG 6 cut(s) 246, 840, 1332, 1362, 1487, 1987
Bme18I GGWCC 3 cut(s) 1460, 1991, 2033
BmgT120I GGNCC 7 cut(s) 863, 896, 992, 993, 1460, 1991, 2033
BmiI GGNNCC 7 cut(s) 44, 844, 864, 994, 1521, 1645, 1992
BmrFI CCNGG 6 cut(s) 246, 840, 1332, 1362, 1487, 1987
BmrI ACTGGG 2 cut(s) 337, 1105
BmsI GCATC 2 cut(s) 802, 1491
BmuI ACTGGG 2 cut(s) 337, 1105
BpiI GAAGAC 1 cut(s) 1125
BpmI CTGGAG 1 cut(s) 748
BpuEI CTTGAG 1 cut(s) 1272
BpuMI CCSGG 4 cut(s) 246, 1332, 1362, 1487
BsaBI GATNNNNATC 3 cut(s) 179, 1481, 1524
BsaHI GRCGYC 1 cut(s) 771
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 5 cut(s) 9, 442, 1331, 1788, 1986
BsaWI WCCGGW 3 cut(s) 1133, 1516, 1757
BsaXI ACNNNNNCTCC 2 cut(s) 746, 776
Bsc4I CCNNNNNNNGG 7 cut(s) 52, 199, 1050, 1133, 1457, 1884, 2000
Bse118I RCCGGY 1 cut(s) 547
Bse1I ACTGG 4 cut(s) 332, 765, 1111, 1913
Bse3DI GCAATG 2 cut(s) 32, 551
Bse8I GATNNNNATC 3 cut(s) 179, 1481, 1524
BseBI CCWGG 2 cut(s) 840, 1987
BseDI CCNNGG 5 cut(s) 9, 442, 1331, 1788, 1986
BseGI GGATG 5 cut(s) 751, 834, 1327, 1482, 2019
BseJI GATNNNNATC 3 cut(s) 179, 1481, 1524
BseLI CCNNNNNNNGG 7 cut(s) 52, 199, 1050, 1133, 1457, 1884, 2000
BseMI GCAATG 2 cut(s) 32, 551
BseMII CTCAG 3 cut(s) 689, 717, 1029
BseNI ACTGG 4 cut(s) 332, 765, 1111, 1913
BseRI GAGGAG 4 cut(s) 46, 264, 1033, 2028
BseSI GKGCMC 1 cut(s) 996
BseXI GCAGC 3 cut(s) 153, 502, 672
BseYI CCCAGC 1 cut(s) 503
Bsh1236I CGCG 3 cut(s) 501, 515, 1244
Bsh1285I CGRYCG 2 cut(s) 57, 1331
BshFI GGCC 9 cut(s) 547, 865, 897, 994, 1040, 1096, 1229, 1787, 1793
BshNI GGYRCC 2 cut(s) 842, 1643
BsiEI CGRYCG 2 cut(s) 57, 1331
BsiHKAI GWGCWC 4 cut(s) 136, 162, 294, 1757
BsiSI CCGG 8 cut(s) 246, 548, 1134, 1331, 1362, 1487, 1517, 1758
BslFI GGGAC 4 cut(s) 438, 1388, 1883, 2004
BslI CCNNNNNNNGG 7 cut(s) 52, 199, 1050, 1133, 1457, 1884, 2000
BsmAI GTCTC 3 cut(s) 246, 660, 1233
BsmBI CGTCTC 1 cut(s) 1233
BsmFI GGGAC 4 cut(s) 438, 1388, 1883, 2004
BsmI GAATGC 1 cut(s) 1268
BsnI GGCC 9 cut(s) 547, 865, 897, 994, 1040, 1096, 1229, 1787, 1793
Bso31I GGTCTC 1 cut(s) 660
Bsp120I GGGCCC 1 cut(s) 992
Bsp1286I GDGCHC 5 cut(s) 136, 162, 294, 996, 1757
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 7 cut(s) 663, 860, 1037, 1230, 1236, 1244, 1563
BspANI GGCC 9 cut(s) 547, 865, 897, 994, 1040, 1096, 1229, 1787, 1793
BspCNI CTCAG 3 cut(s) 688, 718, 1028
BspFNI CGCG 3 cut(s) 501, 515, 1244
BspLI GGNNCC 7 cut(s) 44, 844, 864, 994, 1521, 1645, 1992
BspPI GGATC 9 cut(s) 37, 50, 339, 806, 1153, 1255, 1497, 1514, 1527
BspQI GCTCTTC 2 cut(s) 80, 1919
BspT107I GGYRCC 2 cut(s) 842, 1643
BspTNI GGTCTC 1 cut(s) 660
BsrDI GCAATG 2 cut(s) 32, 551
BsrFI RCCGGY 1 cut(s) 547
BsrI ACTGG 4 cut(s) 332, 765, 1111, 1913
BssAI RCCGGY 1 cut(s) 547
BssECI CCNNGG 5 cut(s) 9, 442, 1331, 1788, 1986
BssNI GRCGYC 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 2 cut(s) 840, 1987
Bst4CI ACNGT 5 cut(s) 202, 913, 1357, 1544, 1658
Bst6I CTCTTC 4 cut(s) 80, 732, 1062, 1919
BstACI GRCGYC 1 cut(s) 771
BstAPI GCANNNNNTGC 1 cut(s) 1211
BstC8I GCNNGC 4 cut(s) 132, 549, 1234, 1785
BstDEI CTNAG 4 cut(s) 675, 726, 920, 1015
BstDSI CCRYGG 1 cut(s) 9
BstENI CCTNNNNNAGG 1 cut(s) 1998
BstF5I GGATG 5 cut(s) 751, 834, 1327, 1482, 2019
BstFNI CGCG 3 cut(s) 501, 515, 1244
BstH2I RGCGCY 1 cut(s) 1784
BstHHI GCGC 3 cut(s) 503, 558, 1783
BstMAI GTCTC 3 cut(s) 246, 660, 1233
BstMCI CGRYCG 2 cut(s) 57, 1331
BstMWI GCNNNNNNNGC 4 cut(s) 553, 871, 1211, 1799
BstNI CCWGG 2 cut(s) 840, 1987
BstSCI CCNGG 6 cut(s) 244, 838, 1330, 1360, 1485, 1985
BstSFI CTRYAG 2 cut(s) 816, 1339
BstSLI GKGCMC 1 cut(s) 996
BstUI CGCG 3 cut(s) 501, 515, 1244
BstV1I GCAGC 3 cut(s) 153, 502, 672
BstV2I GAAGAC 1 cut(s) 1125
BstX2I RGATCY 3 cut(s) 42, 331, 1519
BstXI CCANNNNNNTGG 1 cut(s) 1470
BstYI RGATCY 3 cut(s) 42, 331, 1519
BsuI GTATCC 1 cut(s) 1946
BsuRI GGCC 9 cut(s) 547, 865, 897, 994, 1040, 1096, 1229, 1787, 1793
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 5 cut(s) 751, 834, 1327, 1482, 2019
BtsIMutI CAGTG 5 cut(s) 772, 804, 909, 1663, 1924
Cac8I GCNNGC 4 cut(s) 132, 549, 1234, 1785
CaiI CAGNNNCTG 1 cut(s) 1154
CfoI GCGC 3 cut(s) 503, 558, 1783
Cfr10I RCCGGY 1 cut(s) 547
Cfr13I GGNCC 7 cut(s) 863, 896, 992, 993, 1460, 1991, 2033
CseI GACGC 2 cut(s) 521, 1250
CsiI ACCWGGT 1 cut(s) 838
Csp6I GTAC 7 cut(s) 481, 843, 1384, 1644, 1701, 1906, 2011
CviQI GTAC 7 cut(s) 481, 843, 1384, 1644, 1701, 1906, 2011
DdeI CTNAG 4 cut(s) 675, 726, 920, 1015
DraI TTTAAA 1 cut(s) 733
EaeI YGGCCR 4 cut(s) 545, 1094, 1227, 1791
Eam1104I CTCTTC 4 cut(s) 80, 732, 1062, 1919
EarI CTCTTC 4 cut(s) 80, 732, 1062, 1919
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 2 cut(s) 162, 996
Eco31I GGTCTC 1 cut(s) 660
Eco47I GGWCC 3 cut(s) 1460, 1991, 2033
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 1900
EcoICRI GAGCTC 1 cut(s) 160
EcoNI CCTNNNNNAGG 1 cut(s) 1998
EcoO109I RGGNCCY 2 cut(s) 1991, 2033
EcoRI GAATTC 2 cut(s) 264, 1893
EcoRII CCWGG 2 cut(s) 838, 1985
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 2 cut(s) 162, 996
ErhI CCWWGG 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 1233
FalI AAGNNNNNCTT 6 cut(s) 562, 594, 687, 719, 1198, 1230
FaqI GGGAC 4 cut(s) 438, 1388, 1883, 2004
FauI CCCGC 2 cut(s) 853, 1237
FauNDI CATATG 1 cut(s) 1705
FbaI TGATCA 1 cut(s) 831
FblI GTMKAC 1 cut(s) 535
Fnu4HI GCNGC 5 cut(s) 142, 491, 661, 1038, 1230
FokI GGATG 5 cut(s) 758, 841, 1334, 1469, 2006
FriOI GRGCYC 2 cut(s) 162, 996
Fsp4HI GCNGC 5 cut(s) 142, 491, 661, 1038, 1230
FspBI CTAG 3 cut(s) 1128, 1196, 2027
FspI TGCGCA 1 cut(s) 557
GlaI GCGC 3 cut(s) 502, 557, 1782
GluI GCNGC 5 cut(s) 142, 491, 661, 1038, 1230
GsaI CCCAGC 1 cut(s) 507
GsuI CTGGAG 1 cut(s) 748
HaeII RGCGCY 1 cut(s) 1784
HaeIII GGCC 9 cut(s) 547, 865, 897, 994, 1040, 1096, 1229, 1787, 1793
HapII CCGG 8 cut(s) 246, 548, 1134, 1331, 1362, 1487, 1517, 1758
HgaI GACGC 2 cut(s) 521, 1250
HhaI GCGC 3 cut(s) 503, 558, 1783
Hin1I GRCGYC 1 cut(s) 771
Hin6I GCGC 3 cut(s) 501, 556, 1781
HinP1I GCGC 3 cut(s) 501, 556, 1781
HincII GTYRAC 3 cut(s) 472, 954, 1428
HindII GTYRAC 3 cut(s) 472, 954, 1428
HinfI GANTC 5 cut(s) 228, 636, 1175, 1285, 1818
HpaI GTTAAC 1 cut(s) 1428
HpaII CCGG 8 cut(s) 246, 548, 1134, 1331, 1362, 1487, 1517, 1758
HphI GGTGA 4 cut(s) 31, 458, 1099, 1148
Hpy166II GTNNAC 7 cut(s) 472, 536, 954, 1139, 1428, 1540, 1551
Hpy188I TCNGA 7 cut(s) 227, 622, 678, 1018, 1117, 1452, 2024
Hpy188III TCNNGA 6 cut(s) 123, 692, 725, 937, 1282, 1980
Hpy8I GTNNAC 7 cut(s) 472, 536, 954, 1139, 1428, 1540, 1551
Hpy99I CGWCG 3 cut(s) 118, 397, 938
HpyAV CCTTC 6 cut(s) 10, 120, 976, 1312, 1943, 2024
HpyCH4III ACNGT 5 cut(s) 202, 913, 1357, 1544, 1658
HpyCH4IV ACGT 6 cut(s) 288, 771, 933, 1020, 1718, 1746
HpyCH4V TGCA 7 cut(s) 490, 962, 1061, 1266, 1352, 1810, 2000
HpyF10VI GCNNNNNNNGC 4 cut(s) 553, 871, 1211, 1799
HpyF3I CTNAG 4 cut(s) 675, 726, 920, 1015
HpySE526I ACGT 6 cut(s) 288, 771, 933, 1020, 1718, 1746
Hsp92I GRCGYC 1 cut(s) 771
HspAI GCGC 3 cut(s) 501, 556, 1781
KpnI GGTACC 2 cut(s) 846, 1647
KroI GCCGGC 1 cut(s) 547
KroNI GCCGGC 1 cut(s) 549
Ksp22I TGATCA 1 cut(s) 831
KspAI GTTAAC 1 cut(s) 1428
LguI GCTCTTC 2 cut(s) 80, 1919
LmnI GCTCC 5 cut(s) 205, 1034, 1136, 1752, 1776
Lsp1109I GCAGC 3 cut(s) 153, 502, 672
LweI GCATC 2 cut(s) 802, 1491
MabI ACCWGGT 1 cut(s) 838
MaeI CTAG 3 cut(s) 1128, 1196, 2027
MaeII ACGT 6 cut(s) 288, 771, 933, 1020, 1718, 1746
MaeIII GTNAC 6 cut(s) 323, 385, 767, 926, 1161, 1681
MfeI CAATTG 2 cut(s) 1401, 1775
MflI RGATCY 3 cut(s) 42, 331, 1519
MhlI GDGCHC 5 cut(s) 136, 162, 294, 996, 1757
MlsI TGGCCA 1 cut(s) 1096
MluI ACGCGT 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 1096
MlyI GAGTC 2 cut(s) 237, 1279
MmeI TCCRAC 3 cut(s) 776, 1487, 2047
Mox20I TGGCCA 1 cut(s) 1096
MroNI GCCGGC 1 cut(s) 547
MroXI GAANNNNTTC 1 cut(s) 699
MscI TGGCCA 1 cut(s) 1096
Msp20I TGGCCA 1 cut(s) 1096
MspA1I CMGCKG 2 cut(s) 663, 860
MspI CCGG 8 cut(s) 246, 548, 1134, 1331, 1362, 1487, 1517, 1758
MspR9I CCNGG 6 cut(s) 246, 840, 1332, 1362, 1487, 1987
MunI CAATTG 2 cut(s) 1401, 1775
Mva1269I GAATGC 1 cut(s) 1268
MvaI CCWGG 2 cut(s) 840, 1987
MvnI CGCG 3 cut(s) 501, 515, 1244
MwoI GCNNNNNNNGC 4 cut(s) 553, 871, 1211, 1799
NaeI GCCGGC 1 cut(s) 549
NciI CCSGG 4 cut(s) 246, 1332, 1362, 1487
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 1705
NgoMIV GCCGGC 1 cut(s) 547
NlaIV GGNNCC 7 cut(s) 44, 844, 864, 994, 1521, 1645, 1992
NmeAIII GCCGAG 2 cut(s) 1769, 1956
NmuCI GTSAC 3 cut(s) 767, 926, 1681
NsbI TGCGCA 1 cut(s) 557
PciSI GCTCTTC 2 cut(s) 80, 1919
PctI GAATGC 1 cut(s) 1268
PdiI GCCGGC 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 3 cut(s) 636, 1175, 1818
PfoI TCCNGGA 2 cut(s) 244, 1485
PkrI GCNGC 5 cut(s) 143, 492, 662, 1039, 1231
PleI GAGTC 2 cut(s) 236, 1279
PpsI GAGTC 2 cut(s) 236, 1279
PpuMI RGGWCCY 2 cut(s) 1991, 2033
Psp124BI GAGCTC 1 cut(s) 162
Psp1406I AACGTT 1 cut(s) 1746
Psp5II RGGWCCY 2 cut(s) 1991, 2033
Psp6I CCWGG 2 cut(s) 838, 1985
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 2 cut(s) 838, 1985
PspN4I GGNNCC 7 cut(s) 44, 844, 864, 994, 1521, 1645, 1992
PspOMI GGGCCC 1 cut(s) 992
PspPI GGNCC 7 cut(s) 863, 896, 992, 993, 1460, 1991, 2033
PspPPI RGGWCCY 2 cut(s) 1991, 2033
PstNI CAGNNNCTG 1 cut(s) 1154
PsuI RGATCY 3 cut(s) 42, 331, 1519
RsaI GTAC 7 cut(s) 482, 844, 1385, 1645, 1702, 1907, 2012
RsaNI GTAC 7 cut(s) 481, 843, 1384, 1644, 1701, 1906, 2011
SacI GAGCTC 1 cut(s) 162
SapI GCTCTTC 2 cut(s) 80, 1919
SatI GCNGC 5 cut(s) 142, 491, 661, 1038, 1230
Sau96I GGNCC 7 cut(s) 863, 896, 992, 993, 1460, 1991, 2033
SchI GAGTC 2 cut(s) 237, 1279
ScrFI CCNGG 6 cut(s) 246, 840, 1332, 1362, 1487, 1987
SduI GDGCHC 5 cut(s) 136, 162, 294, 996, 1757
SexAI ACCWGGT 1 cut(s) 838
SfaNI GCATC 2 cut(s) 802, 1491
SfcI CTRYAG 2 cut(s) 816, 1339
SinI GGWCC 3 cut(s) 1460, 1991, 2033
SmlI CTYRAG 1 cut(s) 1287
SmoI CTYRAG 1 cut(s) 1287
SsiI CCGC 7 cut(s) 663, 860, 1037, 1230, 1236, 1244, 1563
SspMI CTAG 3 cut(s) 1128, 1196, 2027
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 6 cut(s) 244, 838, 1330, 1360, 1485, 1985
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 5 cut(s) 202, 913, 1357, 1544, 1658
TaiI ACGT 6 cut(s) 291, 774, 936, 1023, 1721, 1749
TaqI TCGA 7 cut(s) 294, 392, 589, 1327, 1816, 1850, 1939
TaqII GACCGA 2 cut(s) 43, 1482
TatI WGTACW 1 cut(s) 1905
TauI GCSGC 2 cut(s) 1040, 1232
TfiI GAWTC 3 cut(s) 636, 1175, 1818
TscAI CASTG 5 cut(s) 772, 811, 916, 1663, 1924
TseFI GTSAC 3 cut(s) 767, 926, 1681
TseI GCWGC 3 cut(s) 141, 490, 660
Tsp45I GTSAC 3 cut(s) 767, 926, 1681
TspDTI ATGAA 8 cut(s) 598, 628, 779, 1092, 1167, 1331, 1357, 1886
TspGWI ACGGA 1 cut(s) 1274
TspRI CASTG 5 cut(s) 772, 811, 916, 1663, 1924
VpaK11BI GGWCC 3 cut(s) 1460, 1991, 2033
XagI CCTNNNNNAGG 1 cut(s) 1998
XapI RAATTY 4 cut(s) 233, 264, 599, 1893
XmiI GTMKAC 1 cut(s) 535
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 3 cut(s) 1128, 1196, 2027
ZraI GACGTC 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.