FvH4_1g03290

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000523
Physical Location & Seq
Forward (+)
1815489 .. 1817436
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03290.t1

Sequence Viewer

Length: 1860 bp
ATGAGGAAATCGGGTGGGGGGAATCTAAGGAGATTATTATTCGGGGTGATTGTTCTGTGTTGTTTTTGCTTTGTTGATGTTGTGGCAAGATCACCAAGAGGTTATGGACTACCAAGACAAGATGCGGTGGAGATCAAGATGGTAAAAGGTGGAGTTGTGTTGGATAATAAGCTTGTGCAAGTCACTTTTTCTAGGCCTGGTGGGGATGTTATTGGAATAAGGTACGGAGGAATTGACAACTTGCTTGAAACCAATAACGAGGAAGGTAATAGAGGGTACTGGGATGTGGTTTGGAATAAGCCTGGAGAGGCGAAAAACACACAGTACAAGCTACAGGGAACACGGTTTAAAGTGATCACGGCGAGGCCGGACCAAGTTGAAATTTCTTTCACCACCAAGTACAATGCAAACCTCCACCGGGGCGTGTCAGTTCCGGTGAATGTTGATAAAAGGTACATAATGCAGCGAGGACGTTCGGGGTTCTATACATATGCCATCTTCGAGCGTCTTGAAGGGTGGCCAGGAGGGGACATGGAGACGCTTAGAGTGGCTTACAAGCTTAAACAAGCCAACTTTCGCTACATGGCACTATCGGACACTAGGCAAAGGTTCATGCCCACAGCCGAGGACCGAGCACGCGGTAGGCCACTTGCATTTAAAGAAGCTGTTCTCTTGAGCAAGGAGGCAAGTAGTCCTGAATTTGTAGGGGAAGTAGATGATAAATATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATCTCTATGGCACCACCGGTGGGATTTTGGATGATCACACCTAGTGATGAGTCTCAAGTTGGGCTGAATTTTCAAGAAGGTGAGGCATGGAAGAAGGTTTTCGGACCTGTGTTTGTCTATCTTAACTCGGCAGCTCCAAGCTCACATAGTTCTTCAATGATCAGTACATTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTTAAAAGTTGGCCATACAATTTCATTAGTTCTCAAGACTATCCTTCTTCAAATCAACGTGGATCGGTTTCAGGCCAGTTACCAGTACATGATCGATACGTTAAGGGGCTTCACAGGGCGAATTCGGCTTACGTGGGATTGGCTGCACCTGGAGAAGTTGGAACATGGCAGAGGGAAAGCAAGGGATACCAATATTGGACTCAAACCGATGAAAAGGGCTATTTTAACATTGAAAATGTTAGACCCGGGCACTATAGTCTGTATGCATCTGTTCCAGGTATAGTTGGAGACTACAAATATGAGTTTGACATTACAATTGAGCCAGGAAGTAAGATCGAATTGGCTAGTATTACTTACAAACCTCCGAGAATCGGTCCTACATTGTGGGAAATCGGCATCCCTGATCGCTCAGCTGCCGAGTTCTACATACCGGATCCATATCCAACTCTGATGAACAAGTTGTACAAGGGAAGTGATTTGGATAAGTTTAGGCAATACGGTTTGTGGTCACGTTATTATGAGCTTTACCCTCACAATGACCTCATCTACAATGTTGGTGTTAACAATTACAGTGACGACTGGTTCTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGTATGTAGGAACTACATGGCAGATACAGTTTGAACTCGACAATGTGGTCAATCCCGGTAACTATACACTTCAGCTGGCATTGGCATCGGCCACTTATGCAGAGTTGCAAGTTCGAATTAACCATTTGAATGCCGAGCGACCTCTTTTTTCGACAGGGCTAATAGGTGACGATAATGCTATAGCAAGACATGGAATCCATGGTTTATACTGGTTCTGGAGTATTAATGTTCCGAGCATTTTACTACGCGAAGGAAGCAACACAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

620

Amino Acids

70.19

Weight (kDa)

8.09

Isoelectric Point (pI)

32.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 40 - 238 5.7e-72 Rhamnogalacturonate lyase family
fn3_3 PF14686 365 - 437 2.2e-24 Polysaccharide lyase family 4, domain II
CBM-like PF14683 451 - 619 6e-40 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1637
AccB1I GGYRCC 1 cut(s) 778
AccII CGCG 2 cut(s) 639, 1839
AciI CCGC 2 cut(s) 125, 639
AclWI GGATC 4 cut(s) 776, 1042, 1400, 1413
AcoI YGGCCR 5 cut(s) 518, 735, 983, 1588, 1680
AcsI RAATTY 4 cut(s) 381, 698, 835, 1093
AcuI CTGAAG 1 cut(s) 1646
AdeI CACNNNGTG 1 cut(s) 812
AfaI GTAC 9 cut(s) 224, 278, 326, 401, 455, 731, 934, 1059, 1436
AfiI CCNNNNNNNGG 3 cut(s) 418, 788, 1343
AgeI ACCGGT 1 cut(s) 784
AjnI CCWGG 6 cut(s) 196, 301, 520, 1120, 1246, 1294
AluBI AGCT 9 cut(s) 172, 331, 559, 665, 902, 909, 1385, 1495, 1666
AluI AGCT 9 cut(s) 172, 331, 559, 665, 902, 909, 1385, 1495, 1666
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 3 cut(s) 530, 825, 1254
AlwI GGATC 4 cut(s) 776, 1042, 1400, 1413
Ama87I CYCGRG 1 cut(s) 1217
AoxI GGCC 9 cut(s) 194, 365, 518, 644, 735, 983, 1045, 1588, 1680
ApeKI GCWGC 4 cut(s) 463, 899, 1115, 1385
ApoI RAATTY 4 cut(s) 381, 698, 835, 1093
AseI ATTAAT 1 cut(s) 1815
AsiGI ACCGGT 1 cut(s) 784
Asp700I GAANNNNTTC 2 cut(s) 666, 866
AspS9I GGNCC 4 cut(s) 370, 628, 872, 1346
AsuC2I CCSGG 4 cut(s) 419, 1218, 1219, 1647
AsuHPI GGTGA 6 cut(s) 58, 84, 382, 448, 860, 1769
AsuII TTCGAA 1 cut(s) 1705
AvaI CYCGRG 1 cut(s) 1217
AvaII GGWCC 4 cut(s) 370, 628, 872, 1346
BaeGI GKGCMC 1 cut(s) 1224
BalI TGGCCA 2 cut(s) 520, 985
BamHI GGATCC 1 cut(s) 1405
BanI GGYRCC 1 cut(s) 778
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 4 cut(s) 475, 911, 1102, 1372
BccI CCATC 2 cut(s) 133, 503
BceAI ACGGC 1 cut(s) 375
BcgI CGANNNNNNTGC 2 cut(s) 1659, 1693
BciT130I CCWGG 6 cut(s) 198, 303, 522, 1122, 1248, 1296
BciVI GTATCC 1 cut(s) 1151
BclI TGATCA 3 cut(s) 354, 801, 927
BcnI CCSGG 4 cut(s) 419, 1218, 1219, 1647
BcoDI GTCTC 3 cut(s) 530, 825, 1254
BfaI CTAG 5 cut(s) 192, 600, 810, 1317, 1572
BfmI CTRYAG 3 cut(s) 332, 1225, 1770
BfuI GTATCC 1 cut(s) 1151
BisI GCNGC 4 cut(s) 464, 900, 1116, 1386
BlpI GCTNAGC 1 cut(s) 1381
BlsI GCNGC 4 cut(s) 465, 901, 1117, 1387
BmcAI AGTACT 1 cut(s) 731
Bme18I GGWCC 4 cut(s) 370, 628, 872, 1346
BmeT110I CYCGRG 1 cut(s) 1217
BmgT120I GGNCC 4 cut(s) 370, 628, 872, 1346
BmiI GGNNCC 2 cut(s) 780, 1407
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 4 cut(s) 112, 1247, 1377, 1685
BmuI ACTGGG 1 cut(s) 289
BpmI CTGGAG 3 cut(s) 324, 1143, 1828
Bpu1102I GCTNAGC 1 cut(s) 1381
Bpu14I TTCGAA 1 cut(s) 1705
BpuEI CTTGAG 4 cut(s) 694, 807, 990, 1548
BpuMI CCSGG 4 cut(s) 419, 1218, 1219, 1647
Bsa29I ATCGAT 1 cut(s) 1066
BsaAI YACGTR 1 cut(s) 1105
BsaBI GATNNNNATC 2 cut(s) 723, 1410
BsaJI CCNNGG 5 cut(s) 418, 624, 738, 1217, 1789
BsaWI WCCGGW 3 cut(s) 433, 784, 1402
Bsc4I CCNNNNNNNGG 3 cut(s) 418, 788, 1343
Bse118I RCCGGY 2 cut(s) 784, 1590
Bse1I ACTGG 5 cut(s) 284, 1048, 1055, 1556, 1805
Bse8I GATNNNNATC 2 cut(s) 723, 1410
BseBI CCWGG 6 cut(s) 198, 303, 522, 1122, 1248, 1296
BseCI ATCGAT 1 cut(s) 1066
BseDI CCNNGG 5 cut(s) 418, 624, 738, 1217, 1789
BseGI GGATG 4 cut(s) 211, 289, 804, 1368
BseJI GATNNNNATC 2 cut(s) 723, 1410
BseLI CCNNNNNNNGG 3 cut(s) 418, 788, 1343
BseMII CTCAG 1 cut(s) 1395
BseNI ACTGG 5 cut(s) 284, 1048, 1055, 1556, 1805
BseSI GKGCMC 1 cut(s) 1224
BseX3I CGGCCG 1 cut(s) 735
BseXI GCAGC 4 cut(s) 475, 911, 1102, 1372
BsgI GTGCAG 1 cut(s) 1101
Bsh1236I CGCG 2 cut(s) 639, 1839
Bsh1285I CGRYCG 1 cut(s) 738
BshFI GGCC 9 cut(s) 196, 367, 520, 646, 737, 985, 1047, 1590, 1682
BshNI GGYRCC 1 cut(s) 778
BshTI ACCGGT 1 cut(s) 784
BshVI ATCGAT 1 cut(s) 1066
BsiEI CGRYCG 1 cut(s) 738
BsiHKAI GWGCWC 1 cut(s) 637
BsiHKCI CYCGRG 1 cut(s) 1217
BsiSI CCGG 8 cut(s) 368, 418, 434, 785, 1218, 1403, 1591, 1647
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 3 cut(s) 418, 788, 1343
BsmAI GTCTC 3 cut(s) 530, 825, 1254
BsmBI CGTCTC 1 cut(s) 530
BsmFI GGGAC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 1726
BsnI GGCC 9 cut(s) 196, 367, 520, 646, 737, 985, 1047, 1590, 1682
BsoBI CYCGRG 1 cut(s) 1217
Bsp119I TTCGAA 1 cut(s) 1705
Bsp1286I GDGCHC 2 cut(s) 637, 1224
Bsp1407I TGTACA 1 cut(s) 1434
Bsp1720I GCTNAGC 1 cut(s) 1381
Bsp19I CCATGG 1 cut(s) 1789
BspACI CCGC 2 cut(s) 125, 639
BspANI GGCC 9 cut(s) 196, 367, 520, 646, 737, 985, 1047, 1590, 1682
BspCNI CTCAG 1 cut(s) 1394
BspDI ATCGAT 1 cut(s) 1066
BspFNI CGCG 2 cut(s) 639, 1839
BspLI GGNNCC 2 cut(s) 780, 1407
BspPI GGATC 4 cut(s) 776, 1042, 1400, 1413
BspT104I TTCGAA 1 cut(s) 1705
BspT107I GGYRCC 1 cut(s) 778
BsrFI RCCGGY 2 cut(s) 784, 1590
BsrGI TGTACA 1 cut(s) 1434
BsrI ACTGG 5 cut(s) 284, 1048, 1055, 1556, 1805
BssAI RCCGGY 2 cut(s) 784, 1590
BssECI CCNNGG 5 cut(s) 418, 624, 738, 1217, 1789
BssT1I CCWWGG 1 cut(s) 1789
Bst2UI CCWGG 6 cut(s) 198, 303, 522, 1122, 1248, 1296
Bst4CI ACNGT 5 cut(s) 324, 345, 1472, 1544, 1620
BstAUI TGTACA 1 cut(s) 1434
BstBAI YACGTR 1 cut(s) 1105
BstBI TTCGAA 1 cut(s) 1705
BstC8I GCNNGC 2 cut(s) 637, 1668
BstDEI CTNAG 3 cut(s) 26, 542, 1381
BstDSI CCRYGG 1 cut(s) 1789
BstF5I GGATG 4 cut(s) 211, 289, 804, 1368
BstFNI CGCG 2 cut(s) 639, 1839
BstMAI GTCTC 3 cut(s) 530, 825, 1254
BstMCI CGRYCG 1 cut(s) 738
BstMWI GCNNNNNNNGC 3 cut(s) 1097, 1688, 1845
BstNI CCWGG 6 cut(s) 198, 303, 522, 1122, 1248, 1296
BstSFI CTRYAG 3 cut(s) 332, 1225, 1770
BstSLI GKGCMC 1 cut(s) 1224
BstUI CGCG 2 cut(s) 639, 1839
BstV1I GCAGC 4 cut(s) 475, 911, 1102, 1372
BstX2I RGATCY 2 cut(s) 768, 1405
BstYI RGATCY 2 cut(s) 768, 1405
BstZI CGGCCG 1 cut(s) 735
Bsu15I ATCGAT 1 cut(s) 1066
BsuI GTATCC 1 cut(s) 1151
BsuRI GGCC 9 cut(s) 196, 367, 520, 646, 737, 985, 1047, 1590, 1682
BsuTUI ATCGAT 1 cut(s) 1066
BtgI CCRYGG 1 cut(s) 1789
BtsCI GGATG 4 cut(s) 211, 289, 804, 1368
BtsIMutI CAGTG 1 cut(s) 1549
Cac8I GCNNGC 2 cut(s) 637, 1668
Cfr10I RCCGGY 2 cut(s) 784, 1590
Cfr13I GGNCC 4 cut(s) 370, 628, 872, 1346
Cfr9I CCCGGG 1 cut(s) 1217
ClaI ATCGAT 1 cut(s) 1066
CseI GACGC 2 cut(s) 494, 547
Csp6I GTAC 9 cut(s) 223, 277, 325, 400, 454, 730, 933, 1058, 1435
CspAI ACCGGT 1 cut(s) 784
CviQI GTAC 9 cut(s) 223, 277, 325, 400, 454, 730, 933, 1058, 1435
DdeI CTNAG 3 cut(s) 26, 542, 1381
DraI TTTAAA 2 cut(s) 349, 658
DraIII CACNNNGTG 1 cut(s) 812
DrdI GACNNNNNNGTC 1 cut(s) 1637
DseDI GACNNNNNNGTC 1 cut(s) 1637
EaeI YGGCCR 5 cut(s) 518, 735, 983, 1588, 1680
EagI CGGCCG 1 cut(s) 735
EclXI CGGCCG 1 cut(s) 735
Eco130I CCWWGG 1 cut(s) 1789
Eco147I AGGCCT 1 cut(s) 196
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 4 cut(s) 370, 628, 872, 1346
Eco52I CGGCCG 1 cut(s) 735
Eco57I CTGAAG 1 cut(s) 1646
Eco88I CYCGRG 1 cut(s) 1217
EcoRI GAATTC 1 cut(s) 1093
EcoRII CCWGG 6 cut(s) 196, 301, 520, 1120, 1246, 1294
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 1 cut(s) 1789
EcoT22I ATGCAT 1 cut(s) 1240
ErhI CCWWGG 1 cut(s) 1789
Esp3I CGTCTC 1 cut(s) 530
FaqI GGGAC 1 cut(s) 542
FauNDI CATATG 1 cut(s) 490
FbaI TGATCA 3 cut(s) 354, 801, 927
Fnu4HI GCNGC 4 cut(s) 464, 900, 1116, 1386
FokI GGATG 4 cut(s) 218, 296, 811, 1355
Fsp4HI GCNGC 4 cut(s) 464, 900, 1116, 1386
FspBI CTAG 5 cut(s) 192, 600, 810, 1317, 1572
GluI GCNGC 4 cut(s) 464, 900, 1116, 1386
GsuI CTGGAG 3 cut(s) 324, 1143, 1828
HaeIII GGCC 9 cut(s) 196, 367, 520, 646, 737, 985, 1047, 1590, 1682
HapII CCGG 8 cut(s) 368, 418, 434, 785, 1218, 1403, 1591, 1647
HgaI GACGC 2 cut(s) 494, 547
HincII GTYRAC 1 cut(s) 1534
HindII GTYRAC 1 cut(s) 1534
HindIII AAGCTT 2 cut(s) 170, 557
HinfI GANTC 5 cut(s) 22, 818, 1171, 1341, 1785
HpaI GTTAAC 1 cut(s) 1534
HpaII CCGG 8 cut(s) 368, 418, 434, 785, 1218, 1403, 1591, 1647
HphI GGTGA 6 cut(s) 58, 84, 382, 448, 860, 1769
Hpy166II GTNNAC 1 cut(s) 1534
Hpy188I TCNGA 5 cut(s) 595, 872, 1338, 1422, 1824
Hpy188III TCNNGA 7 cut(s) 136, 509, 673, 695, 842, 1007, 1807
Hpy8I GTNNAC 1 cut(s) 1534
HpyAV CCTTC 6 cut(s) 257, 506, 839, 856, 1026, 1835
HpyCH4III ACNGT 5 cut(s) 324, 345, 1472, 1544, 1620
HpyCH4IV ACGT 5 cut(s) 472, 1030, 1071, 1104, 1483
HpyCH4V TGCA 9 cut(s) 178, 407, 463, 653, 760, 1118, 1238, 1691, 1699
HpyF10VI GCNNNNNNNGC 3 cut(s) 1097, 1688, 1845
HpyF3I CTNAG 3 cut(s) 26, 542, 1381
HpySE526I ACGT 5 cut(s) 472, 1030, 1071, 1104, 1483
Ksp22I TGATCA 3 cut(s) 354, 801, 927
KspAI GTTAAC 1 cut(s) 1534
LmnI GCTCC 1 cut(s) 907
Lsp1109I GCAGC 4 cut(s) 475, 911, 1102, 1372
LweI GCATC 4 cut(s) 112, 1247, 1377, 1685
MaeI CTAG 5 cut(s) 192, 600, 810, 1317, 1572
MaeII ACGT 5 cut(s) 472, 1030, 1071, 1104, 1483
MaeIII GTNAC 7 cut(s) 181, 1050, 1479, 1544, 1567, 1649, 1757
MboII GAAGA 5 cut(s) 490, 871, 912, 980, 1011
MfeI CAATTG 1 cut(s) 1287
MflI RGATCY 2 cut(s) 768, 1405
MhlI GDGCHC 2 cut(s) 637, 1224
MlsI TGGCCA 2 cut(s) 520, 985
MluNI TGGCCA 2 cut(s) 520, 985
MlyI GAGTC 2 cut(s) 827, 1165
MmeI TCCRAC 5 cut(s) 141, 746, 1111, 1237, 1439
Mox20I TGGCCA 2 cut(s) 520, 985
Mph1103I ATGCAT 1 cut(s) 1240
MroXI GAANNNNTTC 2 cut(s) 666, 866
MscI TGGCCA 2 cut(s) 520, 985
MslI CAYNNNNRTG 1 cut(s) 1719
Msp20I TGGCCA 2 cut(s) 520, 985
MspA1I CMGCKG 2 cut(s) 1385, 1666
MspI CCGG 8 cut(s) 368, 418, 434, 785, 1218, 1403, 1591, 1647
MunI CAATTG 1 cut(s) 1287
Mva1269I GAATGC 1 cut(s) 1726
MvaI CCWGG 6 cut(s) 198, 303, 522, 1122, 1248, 1296
MvnI CGCG 2 cut(s) 639, 1839
MwoI GCNNNNNNNGC 3 cut(s) 1097, 1688, 1845
NciI CCSGG 4 cut(s) 419, 1218, 1219, 1647
NcoI CCATGG 1 cut(s) 1789
NdeI CATATG 1 cut(s) 490
NlaIV GGNNCC 2 cut(s) 780, 1407
NmeAIII GCCGAG 6 cut(s) 649, 713, 763, 875, 1414, 1750
NmuCI GTSAC 5 cut(s) 181, 1479, 1544, 1567, 1757
NsiI ATGCAT 1 cut(s) 1240
NspV TTCGAA 1 cut(s) 1705
PceI AGGCCT 1 cut(s) 196
PctI GAATGC 1 cut(s) 1726
PdmI GAANNNNTTC 2 cut(s) 666, 866
PfeI GAWTC 3 cut(s) 22, 1341, 1785
PinAI ACCGGT 1 cut(s) 784
PkrI GCNGC 4 cut(s) 465, 901, 1117, 1387
PleI GAGTC 2 cut(s) 826, 1165
PpsI GAGTC 2 cut(s) 826, 1165
Ppu21I YACGTR 1 cut(s) 1105
PshBI ATTAAT 1 cut(s) 1815
Psp6I CCWGG 6 cut(s) 196, 301, 520, 1120, 1246, 1294
PspGI CCWGG 6 cut(s) 196, 301, 520, 1120, 1246, 1294
PspN4I GGNNCC 2 cut(s) 780, 1407
PspPI GGNCC 4 cut(s) 370, 628, 872, 1346
PsrI GAACNNNNNNTAC 2 cut(s) 1418, 1450
PsuI RGATCY 2 cut(s) 768, 1405
PvuII CAGCTG 2 cut(s) 1385, 1666
RsaI GTAC 9 cut(s) 224, 278, 326, 401, 455, 731, 934, 1059, 1436
RsaNI GTAC 9 cut(s) 223, 277, 325, 400, 454, 730, 933, 1058, 1435
RseI CAYNNNNRTG 1 cut(s) 1719
SatI GCNGC 4 cut(s) 464, 900, 1116, 1386
Sau96I GGNCC 4 cut(s) 370, 628, 872, 1346
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 2 cut(s) 827, 1165
SduI GDGCHC 2 cut(s) 637, 1224
SfaNI GCATC 4 cut(s) 112, 1247, 1377, 1685
SfcI CTRYAG 3 cut(s) 332, 1225, 1770
SfuI TTCGAA 1 cut(s) 1705
SgrAI CRCCGGYG 1 cut(s) 784
SinI GGWCC 4 cut(s) 370, 628, 872, 1346
SmaI CCCGGG 1 cut(s) 1219
SmiMI CAYNNNNRTG 1 cut(s) 1719
SmlI CTYRAG 4 cut(s) 673, 822, 1005, 1563
SmoI CTYRAG 4 cut(s) 673, 822, 1005, 1563
SseBI AGGCCT 1 cut(s) 196
SsiI CCGC 2 cut(s) 125, 639
SspI AATATT 1 cut(s) 1166
SspMI CTAG 5 cut(s) 192, 600, 810, 1317, 1572
StuI AGGCCT 1 cut(s) 196
StyI CCWWGG 1 cut(s) 1789
TaaI ACNGT 5 cut(s) 324, 345, 1472, 1544, 1620
TaiI ACGT 5 cut(s) 475, 1033, 1074, 1107, 1486
TaqI TCGA 6 cut(s) 501, 1066, 1308, 1629, 1705, 1742
TaqII GACCGA 2 cut(s) 645, 1334
TatI WGTACW 6 cut(s) 324, 399, 729, 932, 1057, 1434
TfiI GAWTC 3 cut(s) 22, 1341, 1785
TscAI CASTG 1 cut(s) 1549
TseFI GTSAC 5 cut(s) 181, 1479, 1544, 1567, 1757
TseI GCWGC 4 cut(s) 463, 899, 1115, 1385
Tsp45I GTSAC 5 cut(s) 181, 1479, 1544, 1567, 1757
TspDTI ATGAA 4 cut(s) 601, 985, 1197, 1439
TspGWI ACGGA 1 cut(s) 240
TspMI CCCGGG 1 cut(s) 1217
TspRI CASTG 1 cut(s) 1549
VpaK11BI GGWCC 4 cut(s) 370, 628, 872, 1346
VspI ATTAAT 1 cut(s) 1815
XapI RAATTY 4 cut(s) 381, 698, 835, 1093
XmaI CCCGGG 1 cut(s) 1217
XmnI GAANNNNTTC 2 cut(s) 666, 866
XspI CTAG 5 cut(s) 192, 600, 810, 1317, 1572
ZrmI AGTACT 1 cut(s) 731
Zsp2I ATGCAT 1 cut(s) 1240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.