FvH4_1g03300

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000523
Physical Location & Seq
Forward (+)
1819825 .. 1822163
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03300.t1

Sequence Viewer

Length: 2031 bp
ATGAGGAATCTGACGGCGTTATTATTAACGGGGATTGTTCTTTGTTGTTCTTGCTTTGTTGATGGCACTGCGGCAAGATCACCAGGAAGTCATGGACTACCAAGACAAGAAAATGCGGTCGAGATCAAGATGGTCAAAAATGGAGTTGTGTTGGACAATAAGCTTGTTCAGATTACTTTTTCTAATCCTGGTGGGGATGTTATTGCAATAAGTTATGGAGGAATTGACAACCTGCTTGAAATTAACAATGTCGAAGGTGATAGAGGGTACTGGGATGTGGTTTGGGATAACGTCGCAGAGACAGTACACACACAGAACAAACTACCGGGGACACAGTTTAAAGTGATCACGCAGAGTCCGGACCAAGTAGAAATATCTTTCATCACCACATACAATGCCAACCTCCACCGCGGCGATAACGGCGTGACAGTTCCGGTGAACATTGACAAAAGGTACATTTTGCAGCGAGGACGTACCGGTTTCTATACCTATGCCATCTTCGAGCGGCTTCAAGGGTGGCCAGGAGGGCGCATGGAGACCCTTAGAGTGGCTTTCAAGCTTCAAGGAGCCAAGTTTCGCTACATGGCGGTATCGGACACTAGGCAAAGGTTCATGCCAACAGCCCAGGACCGAGCACGCGGTAAGCCACTTGCATATAAAGAAGCTGTTCTCTTGAACAATGAGACCAGCAGTCCTGAATTTGCAGGGGAGGTAGATGATAAGTACCAGTACTCGGCTGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATGGCACCACCGGTGGGATTTTGGATGATCACACCTAGTGACGAGTCTCGTATGGCTGGTCCTTTCAAGCAGGACCTCACCTCCCACGTTGGTCCAACTAGCATGACTGTGTTTTCTTGTTCTCATTATGTTGGGAGAGATGTTGGGTTGACGTTTCGAGAAGGTGAGGCATGGAAGAAGCTTTTCGGACCTGTCTATGTCTATCTTAACTCGGCACCTCCAAGCTCAGATAGTCGTTCCTTGATCCGTACACTATGGAACAATGCCAAGGATCAAATGGTTCAAGAAGTTAAAAGTTGGCCATACAATTTCATTAGTTCTCAAGACTATCCTCCTTCAAATCAACGTGGATCGGTTTCAGGCCAGTTACTAGTAAATGATCGATACATTCCAGGGATTTGGGTGGCAAGTTCGGCTTATGTTGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAAGGAAAGCAAGGGATACCAATTTTGGACTCAAACTGATGCAAAAGGCAATTTCTTCATTGAACATGTTAGACCCGGGCACTATAATTTGTATGCATCTGTTCCAGGGATAGTTGGGGACTACAAATATGAAGTTGACATTATAATTAAGCCGGGAAGTAAAATCCAATTGGGTAGTATTACTTACCAACCTCCCAGGATCGGTCCTACATTGTGGGAAATCGGCATCCCTGATCGCTCAGCTGCCGAGTTCTACATACCAGAACCATATCCAACTCTCACGAACAAGTTGTACAAGGGAAGTGAGTTGGACAAGTTTAGGCAATACGGTTTGTGGGCACGTTATAATGAGCTTTACCCTAAAAATGACCTCATTTACAAAGTTGGTGCTAGCAATTACCGCCACGACTGGTTTTATGCTCAAGTGTGCAGATTTGTAGGAAATAACAAGTATGTAGGAACTACATGGCAGATACAGTTTGAACTCAATGCTGTCAATCCCGGAATCTACACACTTCAACTGGCATTGGCATCTGCCACTTACGCAGAGTTGGAAGTTCGAGTTAACAATGCGAATGCCAAGCCACCTCTTTTTTCAACAGGGCTAATAGGTGACGATAATGCTATAGCAAGACATGGAATCCATGGTTTATACTGGTTCTGGAGTATCAAGATACCGAGCACTTCATTACACAAAGGAAGCAACACAATCTATCTTACTCAGACAAGAGGGGGCACACCTCTCCAAGGAGTCATGTATGACTACATAAGACTAGAAGGACCTGCTGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

677

Amino Acids

75.88

Weight (kDa)

8.45

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 37 - 238 1.1e-68 Rhamnogalacturonate lyase family
fn3_3 PF14686 395 - 468 5e-24 Polysaccharide lyase family 4, domain II
CBM-like PF14683 481 - 669 3.8e-47 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1376, 1578
Acc36I ACCTGC 2 cut(s) 240, 2023
AccB1I GGYRCC 2 cut(s) 778, 988
AccBSI CCGCTC 1 cut(s) 505
AccII CGCG 2 cut(s) 411, 639
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 8 cut(s) 71, 116, 409, 411, 505, 587, 639, 1633
AclWI GGATC 5 cut(s) 1012, 1053, 1132, 1231, 1439
AcoI YGGCCR 2 cut(s) 518, 1073
AcsI RAATTY 1 cut(s) 698
AdeI CACNNNGTG 1 cut(s) 812
AfaI GTAC 8 cut(s) 269, 306, 455, 475, 725, 731, 1024, 1526
AfiI CCNNNNNNNGG 5 cut(s) 547, 733, 788, 1433, 1979
AflIII ACRYGT 1 cut(s) 1297
AgeI ACCGGT 2 cut(s) 476, 784
AhdI GACNNNNNGTC 1 cut(s) 690
AhlI ACTAGT 1 cut(s) 1144
AjnI CCWGG 7 cut(s) 82, 187, 520, 624, 1165, 1336, 1427
AluBI AGCT 7 cut(s) 163, 559, 665, 955, 999, 1475, 1585
AluI AGCT 7 cut(s) 163, 559, 665, 955, 999, 1475, 1585
Alw21I GWGCWC 2 cut(s) 637, 1916
Alw26I GTCTC 4 cut(s) 293, 530, 677, 825
AlwI GGATC 5 cut(s) 1012, 1053, 1132, 1231, 1439
Ama87I CYCGRG 1 cut(s) 1307
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 3 cut(s) 518, 1073, 1135
ApeKI GCWGC 3 cut(s) 463, 1205, 1475
ApoI RAATTY 1 cut(s) 698
AsiGI ACCGGT 2 cut(s) 476, 784
Asp700I GAANNNNTTC 2 cut(s) 666, 956
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
AsuC2I CCSGG 6 cut(s) 327, 1212, 1308, 1309, 1386, 1734
AsuHPI GGTGA 7 cut(s) 72, 269, 376, 448, 844, 950, 1856
AsuNHI GCTAGC 1 cut(s) 1622
AvaI CYCGRG 1 cut(s) 1307
AvaII GGWCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
BaeGI GKGCMC 3 cut(s) 1314, 1573, 1970
BalI TGGCCA 2 cut(s) 520, 1075
BanI GGYRCC 2 cut(s) 778, 988
Bbv12I GWGCWC 2 cut(s) 637, 1916
BbvCI CCTCAGC 1 cut(s) 738
BbvI GCAGC 3 cut(s) 475, 1192, 1462
BccI CCATC 3 cut(s) 56, 124, 503
BceAI ACGGC 2 cut(s) 30, 436
BciT130I CCWGG 7 cut(s) 84, 189, 522, 626, 1167, 1338, 1429
BciVI GTATCC 1 cut(s) 1241
BclI TGATCA 2 cut(s) 345, 801
BcnI CCSGG 6 cut(s) 327, 1212, 1308, 1309, 1386, 1734
BcoDI GTCTC 4 cut(s) 293, 530, 677, 825
BcuI ACTAGT 1 cut(s) 1144
BfaI CTAG 6 cut(s) 600, 810, 873, 1145, 1623, 2006
BfmI CTRYAG 1 cut(s) 1857
BfuAI ACCTGC 2 cut(s) 240, 2023
BfuI GTATCC 1 cut(s) 1241
BglI GCCNNNNNGGC 1 cut(s) 526
BisI GCNGC 6 cut(s) 72, 412, 464, 506, 1206, 1476
BlpI GCTNAGC 1 cut(s) 1471
BlsI GCNGC 6 cut(s) 73, 413, 465, 507, 1207, 1477
BmcAI AGTACT 1 cut(s) 731
Bme18I GGWCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
BmeRI GACNNNNNGTC 1 cut(s) 690
BmeT110I CYCGRG 1 cut(s) 1307
BmgT120I GGNCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
BmiI GGNNCC 3 cut(s) 568, 780, 990
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 4 cut(s) 1261, 1337, 1467, 1772
BmtI GCTAGC 1 cut(s) 1626
BmuI ACTGGG 1 cut(s) 280
BpmI CTGGAG 1 cut(s) 1915
Bpu10I CCTNAGC 1 cut(s) 738
Bpu1102I GCTNAGC 1 cut(s) 1471
BpuEI CTTGAG 2 cut(s) 1080, 1638
BpuMI CCSGG 6 cut(s) 327, 1212, 1308, 1309, 1386, 1734
Bsa29I ATCGAT 1 cut(s) 1156
BsaI GGTCTC 2 cut(s) 530, 677
BsaWI WCCGGW 4 cut(s) 358, 433, 476, 784
BsaXI ACNNNNNCTCC 2 cut(s) 839, 869
Bsc4I CCNNNNNNNGG 5 cut(s) 547, 733, 788, 1433, 1979
Bse118I RCCGGY 2 cut(s) 476, 784
Bse1I ACTGG 6 cut(s) 275, 727, 1138, 1646, 1758, 1892
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 7 cut(s) 84, 189, 522, 626, 1167, 1338, 1429
BseCI ATCGAT 1 cut(s) 1156
BseGI GGATG 4 cut(s) 202, 280, 804, 1458
BseLI CCNNNNNNNGG 5 cut(s) 547, 733, 788, 1433, 1979
BseMII CTCAG 5 cut(s) 729, 1014, 1485, 1967, 2010
BseNI ACTGG 6 cut(s) 275, 727, 1138, 1646, 1758, 1892
BseSI GKGCMC 3 cut(s) 1314, 1573, 1970
BseXI GCAGC 3 cut(s) 475, 1192, 1462
BsgI GTGCAG 2 cut(s) 1191, 1681
Bsh1236I CGCG 2 cut(s) 411, 639
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 520, 1075, 1137
BshNI GGYRCC 2 cut(s) 778, 988
BshTI ACCGGT 2 cut(s) 476, 784
BshVI ATCGAT 1 cut(s) 1156
BsiEI CGRYCG 1 cut(s) 120
BsiHKAI GWGCWC 2 cut(s) 637, 1916
BsiHKCI CYCGRG 1 cut(s) 1307
BsiSI CCGG 9 cut(s) 326, 359, 434, 477, 785, 1212, 1308, 1385, 1734
BslFI GGGAC 2 cut(s) 343, 1364
BslI CCNNNNNNNGG 5 cut(s) 547, 733, 788, 1433, 1979
BsmAI GTCTC 4 cut(s) 293, 530, 677, 825
BsmFI GGGAC 2 cut(s) 343, 1364
BsmI GAATGC 1 cut(s) 1813
BsnI GGCC 3 cut(s) 520, 1075, 1137
Bso31I GGTCTC 2 cut(s) 530, 677
BsoBI CYCGRG 1 cut(s) 1307
Bsp1286I GDGCHC 5 cut(s) 637, 1314, 1573, 1916, 1970
Bsp13I TCCGGA 1 cut(s) 358
Bsp1407I TGTACA 1 cut(s) 1524
Bsp1720I GCTNAGC 1 cut(s) 1471
Bsp19I CCATGG 1 cut(s) 1876
BspACI CCGC 8 cut(s) 71, 116, 409, 411, 505, 587, 639, 1633
BspANI GGCC 3 cut(s) 520, 1075, 1137
BspCNI CTCAG 5 cut(s) 730, 1013, 1484, 1966, 2011
BspDI ATCGAT 1 cut(s) 1156
BspEI TCCGGA 1 cut(s) 358
BspFNI CGCG 2 cut(s) 411, 639
BspLI GGNNCC 3 cut(s) 568, 780, 990
BspMI ACCTGC 2 cut(s) 240, 2023
BspOI GCTAGC 1 cut(s) 1626
BspPI GGATC 5 cut(s) 1012, 1053, 1132, 1231, 1439
BspT107I GGYRCC 2 cut(s) 778, 988
BspTNI GGTCTC 2 cut(s) 530, 677
BsrBI CCGCTC 1 cut(s) 505
BsrFI RCCGGY 2 cut(s) 476, 784
BsrGI TGTACA 1 cut(s) 1524
BsrI ACTGG 6 cut(s) 275, 727, 1138, 1646, 1758, 1892
BssAI RCCGGY 2 cut(s) 476, 784
BssT1I CCWWGG 3 cut(s) 1041, 1876, 1978
Bst2UI CCWGG 7 cut(s) 84, 189, 522, 626, 1167, 1338, 1429
Bst4CI ACNGT 6 cut(s) 304, 336, 430, 883, 1562, 1710
BstAUI TGTACA 1 cut(s) 1524
BstC8I GCNNGC 2 cut(s) 637, 1624
BstDEI CTNAG 6 cut(s) 542, 738, 1000, 1471, 1953, 2019
BstDSI CCRYGG 2 cut(s) 409, 1876
BstENI CCTNNNNNAGG 1 cut(s) 1977
BstF5I GGATG 4 cut(s) 202, 280, 804, 1458
BstFNI CGCG 2 cut(s) 411, 639
BstHHI GCGC 1 cut(s) 531
BstMAI GTCTC 4 cut(s) 293, 530, 677, 825
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 5 cut(s) 420, 526, 1187, 1632, 1775
BstNI CCWGG 7 cut(s) 84, 189, 522, 626, 1167, 1338, 1429
BstNSI RCATGY 1 cut(s) 1301
BstSFI CTRYAG 1 cut(s) 1857
BstSLI GKGCMC 3 cut(s) 1314, 1573, 1970
BstUI CGCG 2 cut(s) 411, 639
BstV1I GCAGC 3 cut(s) 475, 1192, 1462
BstXI CCANNNNNNTGG 1 cut(s) 1173
Bsu15I ATCGAT 1 cut(s) 1156
BsuI GTATCC 1 cut(s) 1241
BsuRI GGCC 3 cut(s) 520, 1075, 1137
BsuTUI ATCGAT 1 cut(s) 1156
BtgI CCRYGG 2 cut(s) 409, 1876
BtsCI GGATG 4 cut(s) 202, 280, 804, 1458
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 66
BveI ACCTGC 2 cut(s) 240, 2023
Cac8I GCNNGC 2 cut(s) 637, 1624
CfoI GCGC 1 cut(s) 531
Cfr10I RCCGGY 2 cut(s) 476, 784
Cfr13I GGNCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
Cfr42I CCGCGG 1 cut(s) 412
Cfr9I CCCGGG 1 cut(s) 1307
ClaI ATCGAT 1 cut(s) 1156
Csp6I GTAC 8 cut(s) 268, 305, 454, 474, 724, 730, 1023, 1525
CspAI ACCGGT 2 cut(s) 476, 784
CviQI GTAC 8 cut(s) 268, 305, 454, 474, 724, 730, 1023, 1525
DdeI CTNAG 6 cut(s) 542, 738, 1000, 1471, 1953, 2019
DraI TTTAAA 1 cut(s) 340
DraIII CACNNNGTG 1 cut(s) 812
DriI GACNNNNNGTC 1 cut(s) 690
EaeI YGGCCR 2 cut(s) 518, 1073
Eam1105I GACNNNNNGTC 1 cut(s) 690
Eco130I CCWWGG 3 cut(s) 1041, 1876, 1978
Eco31I GGTCTC 2 cut(s) 530, 677
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
Eco88I CYCGRG 1 cut(s) 1307
EcoNI CCTNNNNNAGG 1 cut(s) 1977
EcoO109I RGGNCCY 2 cut(s) 847, 2012
EcoRII CCWGG 7 cut(s) 82, 187, 520, 624, 1165, 1336, 1427
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 3 cut(s) 1041, 1876, 1978
EcoT22I ATGCAT 1 cut(s) 1330
ErhI CCWWGG 3 cut(s) 1041, 1876, 1978
FalI AAGNNNNNCTT 2 cut(s) 1174, 1206
FaqI GGGAC 2 cut(s) 343, 1364
FbaI TGATCA 2 cut(s) 345, 801
Fnu4HI GCNGC 6 cut(s) 72, 412, 464, 506, 1206, 1476
FokI GGATG 4 cut(s) 209, 287, 811, 1445
Fsp4HI GCNGC 6 cut(s) 72, 412, 464, 506, 1206, 1476
FspBI CTAG 6 cut(s) 600, 810, 873, 1145, 1623, 2006
GlaI GCGC 1 cut(s) 530
GluI GCNGC 6 cut(s) 72, 412, 464, 506, 1206, 1476
GsuI CTGGAG 1 cut(s) 1915
HaeIII GGCC 3 cut(s) 520, 1075, 1137
HapII CCGG 9 cut(s) 326, 359, 434, 477, 785, 1212, 1308, 1385, 1734
HhaI GCGC 1 cut(s) 531
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 3 cut(s) 924, 1369, 1798
HindII GTYRAC 3 cut(s) 924, 1369, 1798
HindIII AAGCTT 3 cut(s) 161, 557, 953
HinfI GANTC 7 cut(s) 7, 355, 818, 1261, 1737, 1872, 1983
HpaI GTTAAC 1 cut(s) 1798
HpaII CCGG 9 cut(s) 326, 359, 434, 477, 785, 1212, 1308, 1385, 1734
HphI GGTGA 7 cut(s) 72, 269, 376, 448, 844, 950, 1856
Hpy166II GTNNAC 6 cut(s) 307, 439, 924, 1025, 1369, 1798
Hpy188I TCNGA 6 cut(s) 12, 171, 595, 962, 1003, 1956
Hpy8I GTNNAC 6 cut(s) 307, 439, 924, 1025, 1369, 1798
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 4 cut(s) 248, 929, 1119, 2003
HpyCH4III ACNGT 6 cut(s) 304, 336, 430, 883, 1562, 1710
HpyCH4IV ACGT 6 cut(s) 291, 472, 861, 926, 1120, 1573
HpyCH4V TGCA 9 cut(s) 206, 463, 653, 704, 760, 1208, 1274, 1328, 1662
HpyF10VI GCNNNNNNNGC 5 cut(s) 420, 526, 1187, 1632, 1775
HpyF3I CTNAG 6 cut(s) 542, 738, 1000, 1471, 1953, 2019
HpySE526I ACGT 6 cut(s) 291, 472, 861, 926, 1120, 1573
HspAI GCGC 1 cut(s) 529
Kpn2I TCCGGA 1 cut(s) 358
Ksp22I TGATCA 2 cut(s) 345, 801
KspAI GTTAAC 1 cut(s) 1798
KspI CCGCGG 1 cut(s) 412
LmnI GCTCC 1 cut(s) 566
Lsp1109I GCAGC 3 cut(s) 475, 1192, 1462
LweI GCATC 4 cut(s) 1261, 1337, 1467, 1772
MaeI CTAG 6 cut(s) 600, 810, 873, 1145, 1623, 2006
MaeII ACGT 6 cut(s) 291, 472, 861, 926, 1120, 1573
MaeIII GTNAC 4 cut(s) 424, 812, 1140, 1844
MbiI CCGCTC 1 cut(s) 505
MboII GAAGA 3 cut(s) 490, 961, 1279
MfeI CAATTG 1 cut(s) 1400
MhlI GDGCHC 5 cut(s) 637, 1314, 1573, 1916, 1970
MlsI TGGCCA 2 cut(s) 520, 1075
MluNI TGGCCA 2 cut(s) 520, 1075
MlyI GAGTC 4 cut(s) 364, 827, 1255, 1992
MmeI TCCRAC 8 cut(s) 132, 746, 893, 1177, 1201, 1521, 1529, 1764
Mox20I TGGCCA 2 cut(s) 520, 1075
Mph1103I ATGCAT 1 cut(s) 1330
MroI TCCGGA 1 cut(s) 358
MroXI GAANNNNTTC 2 cut(s) 666, 956
MscI TGGCCA 2 cut(s) 520, 1075
MseI TTAA 7 cut(s) 26, 243, 339, 981, 1065, 1380, 1797
MslI CAYNNNNRTG 1 cut(s) 881
Msp20I TGGCCA 2 cut(s) 520, 1075
MspA1I CMGCKG 2 cut(s) 411, 1475
MspI CCGG 9 cut(s) 326, 359, 434, 477, 785, 1212, 1308, 1385, 1734
MunI CAATTG 1 cut(s) 1400
Mva1269I GAATGC 1 cut(s) 1813
MvaI CCWGG 7 cut(s) 84, 189, 522, 626, 1167, 1338, 1429
MvnI CGCG 2 cut(s) 411, 639
MwoI GCNNNNNNNGC 5 cut(s) 420, 526, 1187, 1632, 1775
NciI CCSGG 6 cut(s) 327, 1212, 1308, 1309, 1386, 1734
NcoI CCATGG 1 cut(s) 1876
NheI GCTAGC 1 cut(s) 1622
NlaIV GGNNCC 3 cut(s) 568, 780, 990
NmeAIII GCCGAG 3 cut(s) 713, 965, 1504
NmuCI GTSAC 3 cut(s) 424, 812, 1844
NsiI ATGCAT 1 cut(s) 1330
NspI RCATGY 1 cut(s) 1301
PciI ACATGT 1 cut(s) 1297
PctI GAATGC 1 cut(s) 1813
PdmI GAANNNNTTC 2 cut(s) 666, 956
PfeI GAWTC 3 cut(s) 7, 1737, 1872
PfoI TCCNGGA 1 cut(s) 1732
PinAI ACCGGT 2 cut(s) 476, 784
PkrI GCNGC 6 cut(s) 73, 413, 465, 507, 1207, 1477
PleI GAGTC 4 cut(s) 363, 826, 1255, 1991
PpsI GAGTC 4 cut(s) 363, 826, 1255, 1991
PpuMI RGGWCCY 2 cut(s) 847, 2012
PscI ACATGT 1 cut(s) 1297
PsiI TTATAA 2 cut(s) 1376, 1578
Psp5II RGGWCCY 2 cut(s) 847, 2012
Psp6I CCWGG 7 cut(s) 82, 187, 520, 624, 1165, 1336, 1427
PspGI CCWGG 7 cut(s) 82, 187, 520, 624, 1165, 1336, 1427
PspN4I GGNNCC 3 cut(s) 568, 780, 990
PspPI GGNCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
PspPPI RGGWCCY 2 cut(s) 847, 2012
PsrI GAACNNNNNNTAC 2 cut(s) 1508, 1540
PvuII CAGCTG 1 cut(s) 1475
RsaI GTAC 8 cut(s) 269, 306, 455, 475, 725, 731, 1024, 1526
RsaNI GTAC 8 cut(s) 268, 305, 454, 474, 724, 730, 1023, 1525
RseI CAYNNNNRTG 1 cut(s) 881
SacII CCGCGG 1 cut(s) 412
SaqAI TTAA 7 cut(s) 26, 243, 339, 981, 1065, 1380, 1797
SatI GCNGC 6 cut(s) 72, 412, 464, 506, 1206, 1476
Sau96I GGNCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 4 cut(s) 364, 827, 1255, 1992
SduI GDGCHC 5 cut(s) 637, 1314, 1573, 1916, 1970
SfaNI GCATC 4 cut(s) 1261, 1337, 1467, 1772
SfcI CTRYAG 1 cut(s) 1857
Sfr303I CCGCGG 1 cut(s) 412
SgrAI CRCCGGYG 1 cut(s) 784
SgrBI CCGCGG 1 cut(s) 412
SinI GGWCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
SmaI CCCGGG 1 cut(s) 1309
SmiMI CAYNNNNRTG 1 cut(s) 881
SmlI CTYRAG 2 cut(s) 1095, 1653
SmoI CTYRAG 2 cut(s) 1095, 1653
SpeI ACTAGT 1 cut(s) 1144
SsiI CCGC 8 cut(s) 71, 116, 409, 411, 505, 587, 639, 1633
SspMI CTAG 6 cut(s) 600, 810, 873, 1145, 1623, 2006
StyI CCWWGG 3 cut(s) 1041, 1876, 1978
TaaI ACNGT 6 cut(s) 304, 336, 430, 883, 1562, 1710
TaiI ACGT 6 cut(s) 294, 475, 864, 929, 1123, 1576
TaqI TCGA 6 cut(s) 120, 252, 501, 931, 1156, 1792
TaqII GACCGA 2 cut(s) 645, 1424
TatI WGTACW 3 cut(s) 304, 729, 1524
TauI GCSGC 3 cut(s) 74, 414, 508
TfiI GAWTC 3 cut(s) 7, 1737, 1872
Tru1I TTAA 7 cut(s) 26, 243, 339, 981, 1065, 1380, 1797
Tru9I TTAA 7 cut(s) 26, 243, 339, 981, 1065, 1380, 1797
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 3 cut(s) 424, 812, 1844
TseI GCWGC 3 cut(s) 463, 1205, 1475
Tsp45I GTSAC 3 cut(s) 424, 812, 1844
TspDTI ATGAA 6 cut(s) 370, 601, 1075, 1279, 1377, 1908
TspGWI ACGGA 1 cut(s) 1010
TspMI CCCGGG 1 cut(s) 1307
TspRI CASTG 1 cut(s) 73
VpaK11BI GGWCC 8 cut(s) 361, 628, 833, 847, 866, 962, 1436, 2012
XagI CCTNNNNNAGG 1 cut(s) 1977
XapI RAATTY 1 cut(s) 698
XceI RCATGY 1 cut(s) 1301
XcmI CCANNNNNNNNNTGG 1 cut(s) 1048
XmaI CCCGGG 1 cut(s) 1307
XmnI GAANNNNTTC 2 cut(s) 666, 956
XspI CTAG 6 cut(s) 600, 810, 873, 1145, 1623, 2006
ZrmI AGTACT 1 cut(s) 731
Zsp2I ATGCAT 1 cut(s) 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.