Rh2AG038800

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000523
Physical Location & Seq
Forward (+)
3058968 .. 3060872
1905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG038800.1

Sequence Viewer

Length: 1905 bp
ATGGTAAAAAATGGAGTTGTGTTGGATAATAAGCTTGTTCAACTGACTTTTTCTAATCCTGGTGGGGATGTTATTGGAATAAGTTATGCAGGAATTGACAACGTACTTGCAATTAACAACAAGGAAGGTGATAGAGGGTACTGGGATGTGGTTTGGAATTACCCGGGAGAGGCGAAAAACACACAATACAGACTACAAGGAACGCAATTTAAAGTAATCACGGAGCGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAACGCCAACTCCGGTGTGACAGCTCCGGTGAATGTTGACAGAAGATACATAATGCAGCAAGGACGTTCCGGGTTCTATACCTATGCCATCTTCGAGCGCCTTGAAGGGTGGCCAGGAGGGGACATGGAGACAGTAAGAGTGGCTTACAAGCTTCATCAAGCCAAGTTTCGATATATGGCGCTATCGGACACTAGGCAAAGGTTCATGCCAACACCCGATGACCGAGCACGCGGTAGGCCACTTGCATTCAGAGAAGCTGTTCTACTGAACAATGAGACGAGCAGTCCTGAGCTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCTCCACCGGTGGGATTTTGGATGATCACACCTAGTGATGAGTTTCGTATAGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTCCCTAACTGTGTTTGCTGCTCATCATTATGTCGGTAAAGAAGTTGGGCTGACATTCCAAGAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTTTATCTATCTTAACTCGGCAGCACGAAACACGGATAATAGTCCTTCAACGATCCCTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCATACAATTTCATTAGTTCTCCCGACTTTCCTTCTTCGGATAAACGTGGATCAGTTTCGGGCCAGTTACTAGTACATGATCGATACATTGAAAGTCTTCCAAGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAGGGAAAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTCATTGAAAATATTAGACCCGGTAAATATAATTTGTATGCATCTGTTCCAGGGATAGTTGGAGATTACAAATATGAGGTTGACATTACAATTGAACCAGGAAGTAAAATCCAATTGGCTAGTATTACTTACAAACCTCCGAGAATCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCAGAGTTCTACATACCGGATCCATATCCAACTCTCACGAACAAGTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAGTACGGGTTGTGGGCACGTTATTATGAGCGTTACCCTAACAATGACCTCATCTACCGTGCTGGTGCTAGCAATTACAGTGAGGATTGGTTTTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGTATGTAGGAACTACATGGCAGATACAGTTTGAACTCAAGAATGTGGTCAATCCCGGAAACTATACACTTCAACTGGCCTTGGCATCAGCCACTTATGCAGAATTGCAAGTTCGGGTTAACAATTTGAATGCCAAGCCACCTCTTTTTTCGACAGGGCTAATAGGCGACGACAATGCTATAGCAAGACTTGGAATTCATGGCTTATACAGGTTATGGAGTATTGATGTACCAAGCACTCTACTACGCGAAGGAAGCAACACCATATATCTTACTCAGTCCAGAGGTGGCAATTCACCTTTCCAAGGAGTCATGTATGATTATATAAGACTAGAAGGACCTTCTGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

634

Amino Acids

71.72

Weight (kDa)

5.96

Isoelectric Point (pI)

31.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 5 - 190 2.9e-69 Rhamnogalacturonate lyase family
fn3_3 PF14686 353 - 424 2.1e-24 Polysaccharide lyase family 4, domain II
CBM-like PF14683 437 - 627 1.4e-48 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 226
AccII CGCG 2 cut(s) 495, 1797
AciI CCGC 2 cut(s) 226, 495
AclWI GGATC 5 cut(s) 880, 1000, 1099, 1358, 1371
AcoI YGGCCR 5 cut(s) 227, 374, 591, 941, 1546
AcsI RAATTY 1 cut(s) 1743
AdeI CACNNNGTG 1 cut(s) 677
AfaI GTAC 7 cut(s) 105, 140, 587, 1017, 1394, 1427, 1779
AfiI CCNNNNNNNGG 4 cut(s) 169, 653, 1301, 1853
AgeI ACCGGT 1 cut(s) 649
AhdI GACNNNNNGTC 1 cut(s) 546
AhlI ACTAGT 1 cut(s) 1012
AjnI CCWGG 4 cut(s) 58, 376, 1204, 1252
AluBI AGCT 6 cut(s) 34, 287, 415, 521, 556, 1343
AluI AGCT 6 cut(s) 34, 287, 415, 521, 556, 1343
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 2 cut(s) 386, 533
AlwI GGATC 5 cut(s) 880, 1000, 1099, 1358, 1371
Ama87I CYCGRG 1 cut(s) 163
AoxI GGCC 8 cut(s) 227, 374, 500, 591, 941, 1003, 1546, 1626
ApeKI GCWGC 5 cut(s) 319, 755, 854, 1073, 1343
ApoI RAATTY 1 cut(s) 1743
AsiGI ACCGGT 1 cut(s) 649
Asp700I GAANNNNTTC 2 cut(s) 522, 1038
AspLEI GCGC 2 cut(s) 363, 445
AspS9I GGNCC 7 cut(s) 698, 712, 731, 827, 1003, 1304, 1886
AsuC2I CCSGG 6 cut(s) 164, 165, 334, 1080, 1176, 1605
AsuHPI GGTGA 6 cut(s) 140, 247, 304, 709, 815, 1836
AsuNHI GCTAGC 1 cut(s) 1490
AvaI CYCGRG 1 cut(s) 163
AvaII GGWCC 6 cut(s) 698, 712, 731, 827, 1304, 1886
BaeGI GKGCMC 1 cut(s) 1441
BalI TGGCCA 2 cut(s) 376, 943
BamHI GGATCC 1 cut(s) 1363
BarI GAAGNNNNNNTAC 2 cut(s) 510, 542
BbsI GAAGAC 1 cut(s) 1031
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 5 cut(s) 331, 742, 866, 1060, 1330
BccI CCATC 1 cut(s) 359
BcgI CGANNNNNNTGC 2 cut(s) 1706, 1740
BciT130I CCWGG 4 cut(s) 60, 378, 1206, 1254
BciVI GTATCC 1 cut(s) 1109
BclI TGATCA 1 cut(s) 666
BcnI CCSGG 6 cut(s) 164, 165, 334, 1080, 1176, 1605
BcoDI GTCTC 2 cut(s) 386, 533
BcuI ACTAGT 1 cut(s) 1012
BfaI CTAG 7 cut(s) 456, 675, 1013, 1275, 1491, 1530, 1880
BfmI CTRYAG 2 cut(s) 1344, 1728
BfoI RGCGCY 2 cut(s) 364, 446
BfuI GTATCC 1 cut(s) 1109
BisI GCNGC 6 cut(s) 227, 320, 756, 855, 1074, 1344
BlsI GCNGC 6 cut(s) 228, 321, 757, 856, 1075, 1345
BmcAI AGTACT 1 cut(s) 587
Bme18I GGWCC 6 cut(s) 698, 712, 731, 827, 1304, 1886
BmeRI GACNNNNNGTC 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 163
BmgT120I GGNCC 7 cut(s) 698, 712, 731, 827, 1003, 1304, 1886
BmiI GGNNCC 1 cut(s) 1365
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 2 cut(s) 1205, 1643
BmtI GCTAGC 1 cut(s) 1494
BmuI ACTGGG 1 cut(s) 151
BpiI GAAGAC 1 cut(s) 1031
Bpu10I CCTNAGC 1 cut(s) 552
BpuEI CTTGAG 2 cut(s) 1506, 1571
BpuMI CCSGG 6 cut(s) 164, 165, 334, 1080, 1176, 1605
Bsa29I ATCGAT 1 cut(s) 1024
BsaAI YACGTR 1 cut(s) 1063
BsaBI GATNNNNATC 2 cut(s) 579, 1368
BsaJI CCNNGG 6 cut(s) 163, 594, 1043, 1205, 1629, 1852
BsaWI WCCGGW 4 cut(s) 275, 289, 649, 1360
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 653, 1301, 1853
Bse118I RCCGGY 2 cut(s) 649, 1548
Bse1I ACTGG 3 cut(s) 146, 1006, 1629
Bse8I GATNNNNATC 2 cut(s) 579, 1368
BseBI CCWGG 4 cut(s) 60, 378, 1206, 1254
BseCI ATCGAT 1 cut(s) 1024
BseDI CCNNGG 6 cut(s) 163, 594, 1043, 1205, 1629, 1852
BseGI GGATG 4 cut(s) 73, 151, 577, 669
BseJI GATNNNNATC 2 cut(s) 579, 1368
BseLI CCNNNNNNNGG 4 cut(s) 169, 653, 1301, 1853
BseMII CTCAG 3 cut(s) 543, 1838, 1884
BseNI ACTGG 3 cut(s) 146, 1006, 1629
BseSI GKGCMC 1 cut(s) 1441
BseX3I CGGCCG 1 cut(s) 591
BseXI GCAGC 5 cut(s) 331, 742, 866, 1060, 1330
BsgI GTGCAG 1 cut(s) 1059
Bsh1236I CGCG 2 cut(s) 495, 1797
Bsh1285I CGRYCG 1 cut(s) 594
BshFI GGCC 8 cut(s) 229, 376, 502, 593, 943, 1005, 1548, 1628
BshTI ACCGGT 1 cut(s) 649
BshVI ATCGAT 1 cut(s) 1024
BsiEI CGRYCG 1 cut(s) 594
BsiHKAI GWGCWC 1 cut(s) 493
BsiHKCI CYCGRG 1 cut(s) 163
BslFI GGGAC 1 cut(s) 398
BslI CCNNNNNNNGG 4 cut(s) 169, 653, 1301, 1853
BsmAI GTCTC 2 cut(s) 386, 533
BsmBI CGTCTC 1 cut(s) 533
BsmFI GGGAC 1 cut(s) 398
BsmI GAATGC 3 cut(s) 509, 1326, 1684
BsnI GGCC 8 cut(s) 229, 376, 502, 593, 943, 1005, 1548, 1628
BsoBI CYCGRG 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 493, 1441
Bsp1407I TGTACA 1 cut(s) 1392
Bsp143I GATC 8 cut(s) 666, 885, 913, 992, 1021, 1091, 1333, 1363
BspACI CCGC 2 cut(s) 226, 495
BspANI GGCC 8 cut(s) 229, 376, 502, 593, 943, 1005, 1548, 1628
BspCNI CTCAG 3 cut(s) 544, 1837, 1885
BspDI ATCGAT 1 cut(s) 1024
BspFNI CGCG 2 cut(s) 495, 1797
BspLI GGNNCC 1 cut(s) 1365
BspMAI CTGCAG 1 cut(s) 1348
BspOI GCTAGC 1 cut(s) 1494
BspPI GGATC 5 cut(s) 880, 1000, 1099, 1358, 1371
BsrBI CCGCTC 1 cut(s) 226
BsrFI RCCGGY 2 cut(s) 649, 1548
BsrGI TGTACA 1 cut(s) 1392
BsrI ACTGG 3 cut(s) 146, 1006, 1629
BssAI RCCGGY 2 cut(s) 649, 1548
BssECI CCNNGG 6 cut(s) 163, 594, 1043, 1205, 1629, 1852
BssMI GATC 8 cut(s) 666, 885, 913, 992, 1021, 1091, 1333, 1363
BssT1I CCWWGG 3 cut(s) 1043, 1629, 1852
Bst2UI CCWGG 4 cut(s) 60, 378, 1206, 1254
Bst4CI ACNGT 5 cut(s) 397, 748, 1481, 1502, 1578
BstAUI TGTACA 1 cut(s) 1392
BstBAI YACGTR 1 cut(s) 1063
BstC8I GCNNGC 2 cut(s) 493, 1492
BstDEI CTNAG 3 cut(s) 552, 1824, 1893
BstENI CCTNNNNNAGG 1 cut(s) 1851
BstF5I GGATG 4 cut(s) 73, 151, 577, 669
BstFNI CGCG 2 cut(s) 495, 1797
BstH2I RGCGCY 2 cut(s) 364, 446
BstHHI GCGC 2 cut(s) 363, 445
BstKTI GATC 8 cut(s) 669, 888, 916, 995, 1024, 1094, 1336, 1366
BstMAI GTCTC 2 cut(s) 386, 533
BstMBI GATC 8 cut(s) 666, 885, 913, 992, 1021, 1091, 1333, 1363
BstMCI CGRYCG 1 cut(s) 594
BstMWI GCNNNNNNNGC 2 cut(s) 1646, 1803
BstNI CCWGG 4 cut(s) 60, 378, 1206, 1254
BstSFI CTRYAG 2 cut(s) 1344, 1728
BstSLI GKGCMC 1 cut(s) 1441
BstSNI TACGTA 1 cut(s) 1063
BstUI CGCG 2 cut(s) 495, 1797
BstV1I GCAGC 5 cut(s) 331, 742, 866, 1060, 1330
BstV2I GAAGAC 1 cut(s) 1031
BstX2I RGATCY 1 cut(s) 1363
BstYI RGATCY 1 cut(s) 1363
BstZI CGGCCG 1 cut(s) 591
Bsu15I ATCGAT 1 cut(s) 1024
BsuI GTATCC 1 cut(s) 1109
BsuRI GGCC 8 cut(s) 229, 376, 502, 593, 943, 1005, 1548, 1628
BsuTUI ATCGAT 1 cut(s) 1024
BtsCI GGATG 4 cut(s) 73, 151, 577, 669
BtsIMutI CAGTG 1 cut(s) 1507
Cac8I GCNNGC 2 cut(s) 493, 1492
CfoI GCGC 2 cut(s) 363, 445
Cfr10I RCCGGY 2 cut(s) 649, 1548
Cfr13I GGNCC 7 cut(s) 698, 712, 731, 827, 1003, 1304, 1886
Cfr9I CCCGGG 1 cut(s) 163
ClaI ATCGAT 1 cut(s) 1024
Csp6I GTAC 7 cut(s) 104, 139, 586, 1016, 1393, 1426, 1778
CspAI ACCGGT 1 cut(s) 649
CviAII CATG 8 cut(s) 388, 469, 617, 1019, 1095, 1566, 1748, 1861
CviQI GTAC 7 cut(s) 104, 139, 586, 1016, 1393, 1426, 1778
DdeI CTNAG 3 cut(s) 552, 1824, 1893
DpnI GATC 8 cut(s) 668, 887, 915, 994, 1023, 1093, 1335, 1365
DpnII GATC 8 cut(s) 666, 885, 913, 992, 1021, 1091, 1333, 1363
DraI TTTAAA 1 cut(s) 211
DraIII CACNNNGTG 1 cut(s) 677
DriI GACNNNNNGTC 1 cut(s) 546
EaeI YGGCCR 5 cut(s) 227, 374, 591, 941, 1546
EagI CGGCCG 1 cut(s) 591
Eam1105I GACNNNNNGTC 1 cut(s) 546
EclXI CGGCCG 1 cut(s) 591
Eco105I TACGTA 1 cut(s) 1063
Eco130I CCWWGG 3 cut(s) 1043, 1629, 1852
Eco32I GATATC 1 cut(s) 607
Eco47I GGWCC 6 cut(s) 698, 712, 731, 827, 1304, 1886
Eco52I CGGCCG 1 cut(s) 591
Eco88I CYCGRG 1 cut(s) 163
EcoNI CCTNNNNNAGG 1 cut(s) 1851
EcoO109I RGGNCCY 2 cut(s) 712, 1886
EcoRI GAATTC 1 cut(s) 1743
EcoRII CCWGG 4 cut(s) 58, 376, 1204, 1252
EcoRV GATATC 1 cut(s) 607
EcoT14I CCWWGG 3 cut(s) 1043, 1629, 1852
EcoT22I ATGCAT 1 cut(s) 1198
ErhI CCWWGG 3 cut(s) 1043, 1629, 1852
Esp3I CGTCTC 1 cut(s) 533
FaeI CATG 8 cut(s) 391, 472, 620, 1022, 1098, 1569, 1751, 1864
FalI AAGNNNNNCTT 4 cut(s) 391, 423, 1042, 1074
FaqI GGGAC 1 cut(s) 398
FatI CATG 8 cut(s) 387, 468, 616, 1018, 1094, 1565, 1747, 1860
FbaI TGATCA 1 cut(s) 666
Fnu4HI GCNGC 6 cut(s) 227, 320, 756, 855, 1074, 1344
FokI GGATG 4 cut(s) 80, 158, 584, 676
Fsp4HI GCNGC 6 cut(s) 227, 320, 756, 855, 1074, 1344
FspBI CTAG 7 cut(s) 456, 675, 1013, 1275, 1491, 1530, 1880
GlaI GCGC 2 cut(s) 362, 444
GluI GCNGC 6 cut(s) 227, 320, 756, 855, 1074, 1344
HaeII RGCGCY 2 cut(s) 364, 446
HaeIII GGCC 8 cut(s) 229, 376, 502, 593, 943, 1005, 1548, 1628
HhaI GCGC 2 cut(s) 363, 445
Hin1II CATG 8 cut(s) 391, 472, 620, 1022, 1098, 1569, 1751, 1864
Hin6I GCGC 2 cut(s) 361, 443
HinP1I GCGC 2 cut(s) 361, 443
HincII GTYRAC 3 cut(s) 301, 1237, 1669
HindII GTYRAC 3 cut(s) 301, 1237, 1669
HindIII AAGCTT 2 cut(s) 32, 413
HinfI GANTC 4 cut(s) 1129, 1299, 1402, 1857
HpaI GTTAAC 1 cut(s) 1669
HphI GGTGA 6 cut(s) 140, 247, 304, 709, 815, 1836
Hpy166II GTNNAC 3 cut(s) 301, 1237, 1669
Hpy188I TCNGA 6 cut(s) 451, 515, 827, 982, 1296, 1894
Hpy188III TCNNGA 6 cut(s) 551, 965, 1331, 1381, 1588, 1830
Hpy8I GTNNAC 3 cut(s) 301, 1237, 1669
Hpy99I CGWCG 1 cut(s) 1721
HpyAV CCTTC 8 cut(s) 119, 362, 554, 888, 984, 1793, 1877, 1899
HpyCH4III ACNGT 5 cut(s) 397, 748, 1481, 1502, 1578
HpyCH4IV ACGT 6 cut(s) 102, 328, 726, 988, 1062, 1441
HpyF10VI GCNNNNNNNGC 2 cut(s) 1646, 1803
HpyF3I CTNAG 3 cut(s) 552, 1824, 1893
HpySE526I ACGT 6 cut(s) 102, 328, 726, 988, 1062, 1441
Hsp92II CATG 8 cut(s) 391, 472, 620, 1022, 1098, 1569, 1751, 1864
HspAI GCGC 2 cut(s) 361, 443
Ksp22I TGATCA 1 cut(s) 666
KspAI GTTAAC 1 cut(s) 1669
Kzo9I GATC 8 cut(s) 666, 885, 913, 992, 1021, 1091, 1333, 1363
LmnI GCTCC 2 cut(s) 223, 292
Lsp1109I GCAGC 5 cut(s) 331, 742, 866, 1060, 1330
LweI GCATC 2 cut(s) 1205, 1643
MaeI CTAG 7 cut(s) 456, 675, 1013, 1275, 1491, 1530, 1880
MaeII ACGT 6 cut(s) 102, 328, 726, 988, 1062, 1441
MaeIII GTNAC 4 cut(s) 280, 1008, 1454, 1525
MalI GATC 8 cut(s) 668, 887, 915, 994, 1023, 1093, 1335, 1365
MbiI CCGCTC 1 cut(s) 226
MboI GATC 8 cut(s) 666, 885, 913, 992, 1021, 1091, 1333, 1363
MboII GAAGA 5 cut(s) 318, 346, 938, 969, 1031
MfeI CAATTG 2 cut(s) 1245, 1268
MflI RGATCY 1 cut(s) 1363
MhlI GDGCHC 2 cut(s) 493, 1441
MlsI TGGCCA 2 cut(s) 376, 943
MluNI TGGCCA 2 cut(s) 376, 943
MlyI GAGTC 3 cut(s) 1123, 1411, 1866
MmeI TCCRAC 6 cut(s) 602, 709, 1069, 1195, 1389, 1397
Mox20I TGGCCA 2 cut(s) 376, 943
Mph1103I ATGCAT 1 cut(s) 1198
MroXI GAANNNNTTC 2 cut(s) 522, 1038
MscI TGGCCA 2 cut(s) 376, 943
MseI TTAA 4 cut(s) 114, 210, 846, 1668
MslI CAYNNNNRTG 1 cut(s) 765
Msp20I TGGCCA 2 cut(s) 376, 943
MspA1I CMGCKG 1 cut(s) 1343
MunI CAATTG 2 cut(s) 1245, 1268
Mva1269I GAATGC 3 cut(s) 509, 1326, 1684
MvaI CCWGG 4 cut(s) 60, 378, 1206, 1254
MvnI CGCG 2 cut(s) 495, 1797
MwoI GCNNNNNNNGC 2 cut(s) 1646, 1803
NciI CCSGG 6 cut(s) 164, 165, 334, 1080, 1176, 1605
NdeII GATC 8 cut(s) 666, 885, 913, 992, 1021, 1091, 1333, 1363
NheI GCTAGC 1 cut(s) 1490
NlaIII CATG 8 cut(s) 391, 472, 620, 1022, 1098, 1569, 1751, 1864
NlaIV GGNNCC 1 cut(s) 1365
NmeAIII GCCGAG 3 cut(s) 569, 619, 830
NmuCI GTSAC 2 cut(s) 280, 1525
NsiI ATGCAT 1 cut(s) 1198
PctI GAATGC 3 cut(s) 509, 1326, 1684
PdmI GAANNNNTTC 2 cut(s) 522, 1038
PfeI GAWTC 1 cut(s) 1299
PfoI TCCNGGA 1 cut(s) 1603
PinAI ACCGGT 1 cut(s) 649
PkrI GCNGC 6 cut(s) 228, 321, 757, 856, 1075, 1345
PleI GAGTC 3 cut(s) 1123, 1410, 1865
PpsI GAGTC 3 cut(s) 1123, 1410, 1865
Ppu21I YACGTR 1 cut(s) 1063
PpuMI RGGWCCY 2 cut(s) 712, 1886
Psp5II RGGWCCY 2 cut(s) 712, 1886
Psp6I CCWGG 4 cut(s) 58, 376, 1204, 1252
PspGI CCWGG 4 cut(s) 58, 376, 1204, 1252
PspN4I GGNNCC 1 cut(s) 1365
PspPI GGNCC 7 cut(s) 698, 712, 731, 827, 1003, 1304, 1886
PspPPI RGGWCCY 2 cut(s) 712, 1886
PsrI GAACNNNNNNTAC 2 cut(s) 1376, 1408
PstI CTGCAG 1 cut(s) 1348
PsuI RGATCY 1 cut(s) 1363
PvuII CAGCTG 1 cut(s) 1343
RsaI GTAC 7 cut(s) 105, 140, 587, 1017, 1394, 1427, 1779
RsaNI GTAC 7 cut(s) 104, 139, 586, 1016, 1393, 1426, 1778
RseI CAYNNNNRTG 1 cut(s) 765
SaqAI TTAA 4 cut(s) 114, 210, 846, 1668
SatI GCNGC 6 cut(s) 227, 320, 756, 855, 1074, 1344
Sau3AI GATC 8 cut(s) 666, 885, 913, 992, 1021, 1091, 1333, 1363
Sau96I GGNCC 7 cut(s) 698, 712, 731, 827, 1003, 1304, 1886
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 3 cut(s) 1123, 1411, 1866
SduI GDGCHC 2 cut(s) 493, 1441
SfaNI GCATC 2 cut(s) 1205, 1643
SfcI CTRYAG 2 cut(s) 1344, 1728
SgrAI CRCCGGYG 1 cut(s) 649
SinI GGWCC 6 cut(s) 698, 712, 731, 827, 1304, 1886
SmaI CCCGGG 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 2 cut(s) 1521, 1586
SmoI CTYRAG 2 cut(s) 1521, 1586
SnaBI TACGTA 1 cut(s) 1063
SpeI ACTAGT 1 cut(s) 1012
SsiI CCGC 2 cut(s) 226, 495
SspI AATATT 1 cut(s) 1168
SspMI CTAG 7 cut(s) 456, 675, 1013, 1275, 1491, 1530, 1880
StyI CCWWGG 3 cut(s) 1043, 1629, 1852
TaaI ACNGT 5 cut(s) 397, 748, 1481, 1502, 1578
TaiI ACGT 6 cut(s) 105, 331, 729, 991, 1065, 1444
TaqI TCGA 4 cut(s) 357, 433, 1024, 1700
TaqII GACCGA 2 cut(s) 501, 1292
TatI WGTACW 3 cut(s) 585, 1015, 1392
TauI GCSGC 1 cut(s) 229
TfiI GAWTC 1 cut(s) 1299
Tru1I TTAA 4 cut(s) 114, 210, 846, 1668
Tru9I TTAA 4 cut(s) 114, 210, 846, 1668
TscAI CASTG 1 cut(s) 1507
TseFI GTSAC 2 cut(s) 280, 1525
TseI GCWGC 5 cut(s) 319, 755, 854, 1073, 1343
Tsp45I GTSAC 2 cut(s) 280, 1525
TspDTI ATGAA 6 cut(s) 241, 407, 457, 943, 1155, 1736
TspGWI ACGGA 2 cut(s) 236, 881
TspMI CCCGGG 1 cut(s) 163
TspRI CASTG 1 cut(s) 1507
VpaK11BI GGWCC 6 cut(s) 698, 712, 731, 827, 1304, 1886
XagI CCTNNNNNAGG 1 cut(s) 1851
XapI RAATTY 1 cut(s) 1743
XmaI CCCGGG 1 cut(s) 163
XmnI GAANNNNTTC 2 cut(s) 522, 1038
XspI CTAG 7 cut(s) 456, 675, 1013, 1275, 1491, 1530, 1880
ZrmI AGTACT 1 cut(s) 587
Zsp2I ATGCAT 1 cut(s) 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.