Rw2G003060

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000523
Physical Location & Seq
Forward (+)
2904459 .. 2906489
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003060.1

Sequence Viewer

Length: 2031 bp
ATGAGGAATTCAGGTGGGTGGATTCTGGGGACGTTATTATTTTTGGGTGGTTTTTTCTTTGTTCATGTTGCGGCAAGTTCACCGAGAAGTCATCGACAACTGGCGCAAGATGCGGTCGAGATCAAGATGGTAAAAAATGGAGTTGTGTTGGATAATAAGCTTGTTCAACTGACTTTTTCTAATCCTGGTGGGGATGTTATTGGAATAAGTTATGCAGGAATTGACAACTTACTTGCAATTAACAACAAGGAAGGTGATAGAGGGTACTGGGATGTGGTTTGGAATTACCCGGGAGAGGCGAAAAACACACAATACAGACTACAAGGAACACAATTTAAAGTAATCACGGAGCGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAACGCCAACTCCGGTGTGACAGCTCCGGTGAATGTTGACAGAAGATACATAATGCAGCAAGGACGTTCCGGGTTCTATACCTATGCCATCTTCGAGCGCCTTGAAGGGTGGCCAGGAGGGGACATGGAGACAGTAAGAGTGGCTTACAAGCTTCATCAAGCCAAGTTTCGATATATGGCGCTATCGGACACTAGGCAAAGGTTCATGCCAACAGCCGATGACCGAGCACGCGGTAGGCCACTTGCATTCAGAGAAGCTGTTCTACTGAACAATGAGACGAGCAGTCCTGAACTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCTCCACCGGTGGGATTTTGGATGATCACACCTAGTGATGAGTTTCGTATAGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTCCCTAACTGTGTTTGCTGCTCATCATTATGTCGGCAAAGAAGTTGGGCTGACATTCCAAAAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTTTATCTATCTTAACTCGGCAGCACGAAACACAGATAATAGTCCTTCAATGATCCCTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCGTACAATTTCATTAGTTCTCCCGACTTTCCTTCTTCGGATAAACGTGGATCAGTTTCGGGCCAGTTACTAGTACATGATCGATACATTGAAAGTCTTCCAAGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAGGGAAAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTCATTGAAAATATTAGACCCGGTAAATATAGTTTGTATGCATCTGTTCCAGGGATAGTTGGAGATTACAAATATGAGGTTGACATTACAATTGAACCAGGAAGTAAAATCCAATTGGCTAGTATTACTTACAAACCTCCGAGAACCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCAGAGTTCTACATACCGGATCCATATCCAACTCTCACGAACAAGTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAGTACGGGTTGTGGGCACGTTATTATGAGCGTTACCCTAACAATGACCTCATCTACCGTGTTGGTGCTAGCAATTACAGTGAGGATTGGTTTTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGTATGTAGGAACTACATGGCAGATACAGTTTGAACTCAAGAATGTGGTCAATCCCGGAAACTATACACTTCAACTGGCCTTGGCATCAGCCACTTATGCAGAATTGCAAGTTCGGGTTAACAATTTGAATGCCAAGCCACCTCTTTTTTCGACAGGGCTAATAGGCGACGACAATGCTATAGCAAGACTTGGAATTCATGGCTTATACAGGTTATGGAGTATTGATGTACCAAGCACTCTACTACGCGAAGGAAGCAACACCATCTATCTTACTCAGTCCAGAGGTGGCAATTCACCTTTCCAAGGAGTCATGTATGATTATATAAGACTAGAAGGACCTTCTGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

676

Amino Acids

76.33

Weight (kDa)

6.79

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 33 - 232 9.1e-71 Rhamnogalacturonate lyase family
fn3_3 PF14686 395 - 466 7.7e-24 Polysaccharide lyase family 4, domain II
CBM-like PF14683 479 - 669 1.3e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 352
AccII CGCG 2 cut(s) 621, 1923
AciI CCGC 4 cut(s) 71, 113, 352, 621
AclWI GGATC 5 cut(s) 1006, 1126, 1225, 1484, 1497
AcoI YGGCCR 5 cut(s) 353, 500, 717, 1067, 1672
AcsI RAATTY 2 cut(s) 7, 1869
AdeI CACNNNGTG 1 cut(s) 803
AfaI GTAC 7 cut(s) 266, 713, 1073, 1143, 1520, 1553, 1905
AfiI CCNNNNNNNGG 4 cut(s) 295, 779, 1427, 1979
AgeI ACCGGT 2 cut(s) 775, 1427
AhdI GACNNNNNGTC 1 cut(s) 672
AhlI ACTAGT 1 cut(s) 1138
AjnI CCWGG 4 cut(s) 184, 502, 1330, 1378
AluBI AGCT 5 cut(s) 160, 413, 541, 647, 1469
AluI AGCT 5 cut(s) 160, 413, 541, 647, 1469
Alw21I GWGCWC 1 cut(s) 619
Alw26I GTCTC 2 cut(s) 512, 659
AlwI GGATC 5 cut(s) 1006, 1126, 1225, 1484, 1497
Ama87I CYCGRG 1 cut(s) 289
AoxI GGCC 8 cut(s) 353, 500, 626, 717, 1067, 1129, 1672, 1752
ApeKI GCWGC 5 cut(s) 445, 881, 980, 1199, 1469
ApoI RAATTY 2 cut(s) 7, 1869
AsiGI ACCGGT 2 cut(s) 775, 1427
Asp700I GAANNNNTTC 2 cut(s) 648, 1164
AspLEI GCGC 3 cut(s) 106, 489, 571
AspS9I GGNCC 7 cut(s) 824, 838, 857, 953, 1129, 1430, 2012
AsuC2I CCSGG 6 cut(s) 290, 291, 460, 1206, 1302, 1731
AsuHPI GGTGA 7 cut(s) 72, 266, 373, 430, 835, 941, 1962
AsuNHI GCTAGC 1 cut(s) 1616
AvaI CYCGRG 1 cut(s) 289
AvaII GGWCC 6 cut(s) 824, 838, 857, 953, 1430, 2012
BaeGI GKGCMC 1 cut(s) 1567
BalI TGGCCA 1 cut(s) 502
BamHI GGATCC 1 cut(s) 1489
BarI GAAGNNNNNNTAC 2 cut(s) 636, 668
BbsI GAAGAC 1 cut(s) 1157
Bbv12I GWGCWC 1 cut(s) 619
BbvI GCAGC 5 cut(s) 457, 868, 992, 1186, 1456
BccI CCATC 3 cut(s) 121, 485, 1946
BceAI ACGGC 1 cut(s) 1054
BcgI CGANNNNNNTGC 2 cut(s) 1832, 1866
BciT130I CCWGG 4 cut(s) 186, 504, 1332, 1380
BciVI GTATCC 1 cut(s) 1235
BclI TGATCA 1 cut(s) 792
BcnI CCSGG 6 cut(s) 290, 291, 460, 1206, 1302, 1731
BcoDI GTCTC 2 cut(s) 512, 659
BcuI ACTAGT 1 cut(s) 1138
BfaI CTAG 7 cut(s) 582, 801, 1139, 1401, 1617, 1656, 2006
BfmI CTRYAG 2 cut(s) 1470, 1854
BfoI RGCGCY 2 cut(s) 490, 572
BfuI GTATCC 1 cut(s) 1235
BisI GCNGC 7 cut(s) 72, 353, 446, 882, 981, 1200, 1470
BlsI GCNGC 7 cut(s) 73, 354, 447, 883, 982, 1201, 1471
BmcAI AGTACT 1 cut(s) 713
Bme18I GGWCC 6 cut(s) 824, 838, 857, 953, 1430, 2012
BmeRI GACNNNNNGTC 1 cut(s) 672
BmeT110I CYCGRG 1 cut(s) 289
BmgT120I GGNCC 7 cut(s) 824, 838, 857, 953, 1129, 1430, 2012
BmiI GGNNCC 1 cut(s) 1491
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 3 cut(s) 100, 1331, 1769
BmtI GCTAGC 1 cut(s) 1620
BmuI ACTGGG 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 1157
BpuEI CTTGAG 2 cut(s) 1632, 1697
BpuMI CCSGG 6 cut(s) 290, 291, 460, 1206, 1302, 1731
Bsa29I ATCGAT 1 cut(s) 1150
BsaAI YACGTR 1 cut(s) 1189
BsaBI GATNNNNATC 2 cut(s) 705, 1494
BsaJI CCNNGG 6 cut(s) 289, 720, 1169, 1331, 1755, 1978
BsaWI WCCGGW 5 cut(s) 401, 415, 775, 1427, 1486
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 4 cut(s) 295, 779, 1427, 1979
Bse118I RCCGGY 3 cut(s) 775, 1427, 1674
Bse1I ACTGG 4 cut(s) 105, 272, 1132, 1755
Bse8I GATNNNNATC 2 cut(s) 705, 1494
BseBI CCWGG 4 cut(s) 186, 504, 1332, 1380
BseCI ATCGAT 1 cut(s) 1150
BseDI CCNNGG 6 cut(s) 289, 720, 1169, 1331, 1755, 1978
BseGI GGATG 4 cut(s) 199, 277, 703, 795
BseJI GATNNNNATC 2 cut(s) 705, 1494
BseLI CCNNNNNNNGG 4 cut(s) 295, 779, 1427, 1979
BseMII CTCAG 2 cut(s) 1964, 2010
BseNI ACTGG 4 cut(s) 105, 272, 1132, 1755
BseSI GKGCMC 1 cut(s) 1567
BseX3I CGGCCG 1 cut(s) 717
BseXI GCAGC 5 cut(s) 457, 868, 992, 1186, 1456
BsgI GTGCAG 1 cut(s) 1185
Bsh1236I CGCG 2 cut(s) 621, 1923
Bsh1285I CGRYCG 2 cut(s) 117, 720
BshFI GGCC 8 cut(s) 355, 502, 628, 719, 1069, 1131, 1674, 1754
BshTI ACCGGT 2 cut(s) 775, 1427
BshVI ATCGAT 1 cut(s) 1150
BsiEI CGRYCG 2 cut(s) 117, 720
BsiHKAI GWGCWC 1 cut(s) 619
BsiHKCI CYCGRG 1 cut(s) 289
BslFI GGGAC 2 cut(s) 43, 524
BslI CCNNNNNNNGG 4 cut(s) 295, 779, 1427, 1979
BsmAI GTCTC 2 cut(s) 512, 659
BsmBI CGTCTC 1 cut(s) 659
BsmFI GGGAC 2 cut(s) 43, 524
BsmI GAATGC 3 cut(s) 635, 1452, 1810
BsnI GGCC 8 cut(s) 355, 502, 628, 719, 1069, 1131, 1674, 1754
BsoBI CYCGRG 1 cut(s) 289
Bsp1286I GDGCHC 2 cut(s) 619, 1567
Bsp1407I TGTACA 1 cut(s) 1518
Bsp143I GATC 9 cut(s) 120, 792, 1011, 1039, 1118, 1147, 1217, 1459, 1489
BspACI CCGC 4 cut(s) 71, 113, 352, 621
BspANI GGCC 8 cut(s) 355, 502, 628, 719, 1069, 1131, 1674, 1754
BspCNI CTCAG 2 cut(s) 1963, 2011
BspDI ATCGAT 1 cut(s) 1150
BspFNI CGCG 2 cut(s) 621, 1923
BspLI GGNNCC 1 cut(s) 1491
BspMAI CTGCAG 1 cut(s) 1474
BspOI GCTAGC 1 cut(s) 1620
BspPI GGATC 5 cut(s) 1006, 1126, 1225, 1484, 1497
BsrBI CCGCTC 1 cut(s) 352
BsrFI RCCGGY 3 cut(s) 775, 1427, 1674
BsrGI TGTACA 1 cut(s) 1518
BsrI ACTGG 4 cut(s) 105, 272, 1132, 1755
BssAI RCCGGY 3 cut(s) 775, 1427, 1674
BssECI CCNNGG 6 cut(s) 289, 720, 1169, 1331, 1755, 1978
BssMI GATC 9 cut(s) 120, 792, 1011, 1039, 1118, 1147, 1217, 1459, 1489
BssT1I CCWWGG 3 cut(s) 1169, 1755, 1978
Bst2UI CCWGG 4 cut(s) 186, 504, 1332, 1380
Bst4CI ACNGT 5 cut(s) 523, 874, 1607, 1628, 1704
BstAUI TGTACA 1 cut(s) 1518
BstBAI YACGTR 1 cut(s) 1189
BstC8I GCNNGC 2 cut(s) 619, 1618
BstDEI CTNAG 2 cut(s) 1950, 2019
BstENI CCTNNNNNAGG 1 cut(s) 1977
BstF5I GGATG 4 cut(s) 199, 277, 703, 795
BstFNI CGCG 2 cut(s) 621, 1923
BstH2I RGCGCY 2 cut(s) 490, 572
BstHHI GCGC 3 cut(s) 106, 489, 571
BstKTI GATC 9 cut(s) 123, 795, 1014, 1042, 1121, 1150, 1220, 1462, 1492
BstMAI GTCTC 2 cut(s) 512, 659
BstMBI GATC 9 cut(s) 120, 792, 1011, 1039, 1118, 1147, 1217, 1459, 1489
BstMCI CGRYCG 2 cut(s) 117, 720
BstMWI GCNNNNNNNGC 3 cut(s) 110, 1772, 1929
BstNI CCWGG 4 cut(s) 186, 504, 1332, 1380
BstSFI CTRYAG 2 cut(s) 1470, 1854
BstSLI GKGCMC 1 cut(s) 1567
BstSNI TACGTA 1 cut(s) 1189
BstUI CGCG 2 cut(s) 621, 1923
BstV1I GCAGC 5 cut(s) 457, 868, 992, 1186, 1456
BstV2I GAAGAC 1 cut(s) 1157
BstX2I RGATCY 1 cut(s) 1489
BstYI RGATCY 1 cut(s) 1489
BstZI CGGCCG 1 cut(s) 717
Bsu15I ATCGAT 1 cut(s) 1150
BsuI GTATCC 1 cut(s) 1235
BsuRI GGCC 8 cut(s) 355, 502, 628, 719, 1069, 1131, 1674, 1754
BsuTUI ATCGAT 1 cut(s) 1150
BtsCI GGATG 4 cut(s) 199, 277, 703, 795
BtsIMutI CAGTG 1 cut(s) 1633
Cac8I GCNNGC 2 cut(s) 619, 1618
CfoI GCGC 3 cut(s) 106, 489, 571
Cfr10I RCCGGY 3 cut(s) 775, 1427, 1674
Cfr13I GGNCC 7 cut(s) 824, 838, 857, 953, 1129, 1430, 2012
Cfr9I CCCGGG 1 cut(s) 289
ClaI ATCGAT 1 cut(s) 1150
Csp6I GTAC 7 cut(s) 265, 712, 1072, 1142, 1519, 1552, 1904
CspAI ACCGGT 2 cut(s) 775, 1427
CviAII CATG 9 cut(s) 65, 514, 595, 743, 1145, 1221, 1692, 1874, 1987
CviQI GTAC 7 cut(s) 265, 712, 1072, 1142, 1519, 1552, 1904
DdeI CTNAG 2 cut(s) 1950, 2019
DpnI GATC 9 cut(s) 122, 794, 1013, 1041, 1120, 1149, 1219, 1461, 1491
DpnII GATC 9 cut(s) 120, 792, 1011, 1039, 1118, 1147, 1217, 1459, 1489
DraI TTTAAA 1 cut(s) 337
DraIII CACNNNGTG 1 cut(s) 803
DriI GACNNNNNGTC 1 cut(s) 672
EaeI YGGCCR 5 cut(s) 353, 500, 717, 1067, 1672
EagI CGGCCG 1 cut(s) 717
Eam1105I GACNNNNNGTC 1 cut(s) 672
EclXI CGGCCG 1 cut(s) 717
Eco105I TACGTA 1 cut(s) 1189
Eco130I CCWWGG 3 cut(s) 1169, 1755, 1978
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 6 cut(s) 824, 838, 857, 953, 1430, 2012
Eco52I CGGCCG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 1977
EcoO109I RGGNCCY 2 cut(s) 838, 2012
EcoRI GAATTC 2 cut(s) 7, 1869
EcoRII CCWGG 4 cut(s) 184, 502, 1330, 1378
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 3 cut(s) 1169, 1755, 1978
EcoT22I ATGCAT 1 cut(s) 1324
ErhI CCWWGG 3 cut(s) 1169, 1755, 1978
Esp3I CGTCTC 1 cut(s) 659
FaeI CATG 9 cut(s) 68, 517, 598, 746, 1148, 1224, 1695, 1877, 1990
FalI AAGNNNNNCTT 4 cut(s) 517, 549, 1168, 1200
FaqI GGGAC 2 cut(s) 43, 524
FatI CATG 9 cut(s) 64, 513, 594, 742, 1144, 1220, 1691, 1873, 1986
FbaI TGATCA 1 cut(s) 792
Fnu4HI GCNGC 7 cut(s) 72, 353, 446, 882, 981, 1200, 1470
FokI GGATG 4 cut(s) 206, 284, 710, 802
Fsp4HI GCNGC 7 cut(s) 72, 353, 446, 882, 981, 1200, 1470
FspBI CTAG 7 cut(s) 582, 801, 1139, 1401, 1617, 1656, 2006
GlaI GCGC 3 cut(s) 105, 488, 570
GluI GCNGC 7 cut(s) 72, 353, 446, 882, 981, 1200, 1470
HaeII RGCGCY 2 cut(s) 490, 572
HaeIII GGCC 8 cut(s) 355, 502, 628, 719, 1069, 1131, 1674, 1754
HhaI GCGC 3 cut(s) 106, 489, 571
Hin1II CATG 9 cut(s) 68, 517, 598, 746, 1148, 1224, 1695, 1877, 1990
Hin6I GCGC 3 cut(s) 104, 487, 569
HinP1I GCGC 3 cut(s) 104, 487, 569
HincII GTYRAC 3 cut(s) 427, 1363, 1795
HindII GTYRAC 3 cut(s) 427, 1363, 1795
HindIII AAGCTT 2 cut(s) 158, 539
HinfI GANTC 4 cut(s) 22, 1255, 1528, 1983
HpaI GTTAAC 1 cut(s) 1795
HphI GGTGA 7 cut(s) 72, 266, 373, 430, 835, 941, 1962
Hpy166II GTNNAC 4 cut(s) 80, 427, 1363, 1795
Hpy188I TCNGA 6 cut(s) 577, 641, 953, 1108, 1422, 2020
Hpy188III TCNNGA 8 cut(s) 118, 124, 677, 1091, 1457, 1507, 1714, 1956
Hpy8I GTNNAC 4 cut(s) 80, 427, 1363, 1795
Hpy99I CGWCG 1 cut(s) 1847
HpyAV CCTTC 8 cut(s) 245, 488, 680, 1014, 1110, 1919, 2003, 2025
HpyCH4III ACNGT 5 cut(s) 523, 874, 1607, 1628, 1704
HpyCH4IV ACGT 6 cut(s) 32, 454, 852, 1114, 1188, 1567
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 1772, 1929
HpyF3I CTNAG 2 cut(s) 1950, 2019
HpySE526I ACGT 6 cut(s) 32, 454, 852, 1114, 1188, 1567
Hsp92II CATG 9 cut(s) 68, 517, 598, 746, 1148, 1224, 1695, 1877, 1990
HspAI GCGC 3 cut(s) 104, 487, 569
Ksp22I TGATCA 1 cut(s) 792
KspAI GTTAAC 1 cut(s) 1795
Kzo9I GATC 9 cut(s) 120, 792, 1011, 1039, 1118, 1147, 1217, 1459, 1489
LmnI GCTCC 2 cut(s) 349, 418
Lsp1109I GCAGC 5 cut(s) 457, 868, 992, 1186, 1456
LweI GCATC 3 cut(s) 100, 1331, 1769
MaeI CTAG 7 cut(s) 582, 801, 1139, 1401, 1617, 1656, 2006
MaeII ACGT 6 cut(s) 32, 454, 852, 1114, 1188, 1567
MaeIII GTNAC 4 cut(s) 406, 1134, 1580, 1651
MalI GATC 9 cut(s) 122, 794, 1013, 1041, 1120, 1149, 1219, 1461, 1491
MbiI CCGCTC 1 cut(s) 352
MboI GATC 9 cut(s) 120, 792, 1011, 1039, 1118, 1147, 1217, 1459, 1489
MboII GAAGA 5 cut(s) 444, 472, 1064, 1095, 1157
MfeI CAATTG 2 cut(s) 1371, 1394
MflI RGATCY 1 cut(s) 1489
MhlI GDGCHC 2 cut(s) 619, 1567
MlsI TGGCCA 1 cut(s) 502
MluNI TGGCCA 1 cut(s) 502
MlyI GAGTC 3 cut(s) 1249, 1537, 1992
MmeI TCCRAC 7 cut(s) 129, 728, 835, 1195, 1321, 1515, 1523
Mox20I TGGCCA 1 cut(s) 502
Mph1103I ATGCAT 1 cut(s) 1324
MroXI GAANNNNTTC 2 cut(s) 648, 1164
MscI TGGCCA 1 cut(s) 502
MseI TTAA 4 cut(s) 240, 336, 972, 1794
MslI CAYNNNNRTG 1 cut(s) 891
Msp20I TGGCCA 1 cut(s) 502
MspA1I CMGCKG 1 cut(s) 1469
MunI CAATTG 2 cut(s) 1371, 1394
Mva1269I GAATGC 3 cut(s) 635, 1452, 1810
MvaI CCWGG 4 cut(s) 186, 504, 1332, 1380
MvnI CGCG 2 cut(s) 621, 1923
MwoI GCNNNNNNNGC 3 cut(s) 110, 1772, 1929
NciI CCSGG 6 cut(s) 290, 291, 460, 1206, 1302, 1731
NdeII GATC 9 cut(s) 120, 792, 1011, 1039, 1118, 1147, 1217, 1459, 1489
NheI GCTAGC 1 cut(s) 1616
NlaIII CATG 9 cut(s) 68, 517, 598, 746, 1148, 1224, 1695, 1877, 1990
NlaIV GGNNCC 1 cut(s) 1491
NmeAIII GCCGAG 3 cut(s) 695, 745, 956
NmuCI GTSAC 2 cut(s) 406, 1651
NsiI ATGCAT 1 cut(s) 1324
PctI GAATGC 3 cut(s) 635, 1452, 1810
PdmI GAANNNNTTC 2 cut(s) 648, 1164
PfeI GAWTC 1 cut(s) 22
PfoI TCCNGGA 1 cut(s) 1729
PinAI ACCGGT 2 cut(s) 775, 1427
PkrI GCNGC 7 cut(s) 73, 354, 447, 883, 982, 1201, 1471
PleI GAGTC 3 cut(s) 1249, 1536, 1991
PpsI GAGTC 3 cut(s) 1249, 1536, 1991
Ppu21I YACGTR 1 cut(s) 1189
PpuMI RGGWCCY 2 cut(s) 838, 2012
Psp5II RGGWCCY 2 cut(s) 838, 2012
Psp6I CCWGG 4 cut(s) 184, 502, 1330, 1378
PspGI CCWGG 4 cut(s) 184, 502, 1330, 1378
PspN4I GGNNCC 1 cut(s) 1491
PspPI GGNCC 7 cut(s) 824, 838, 857, 953, 1129, 1430, 2012
PspPPI RGGWCCY 2 cut(s) 838, 2012
PsrI GAACNNNNNNTAC 4 cut(s) 1417, 1449, 1502, 1534
PstI CTGCAG 1 cut(s) 1474
PsuI RGATCY 1 cut(s) 1489
PvuII CAGCTG 1 cut(s) 1469
RsaI GTAC 7 cut(s) 266, 713, 1073, 1143, 1520, 1553, 1905
RsaNI GTAC 7 cut(s) 265, 712, 1072, 1142, 1519, 1552, 1904
RseI CAYNNNNRTG 1 cut(s) 891
SaqAI TTAA 4 cut(s) 240, 336, 972, 1794
SatI GCNGC 7 cut(s) 72, 353, 446, 882, 981, 1200, 1470
Sau3AI GATC 9 cut(s) 120, 792, 1011, 1039, 1118, 1147, 1217, 1459, 1489
Sau96I GGNCC 7 cut(s) 824, 838, 857, 953, 1129, 1430, 2012
ScaI AGTACT 1 cut(s) 713
SchI GAGTC 3 cut(s) 1249, 1537, 1992
SduI GDGCHC 2 cut(s) 619, 1567
SfaNI GCATC 3 cut(s) 100, 1331, 1769
SfcI CTRYAG 2 cut(s) 1470, 1854
SgrAI CRCCGGYG 1 cut(s) 775
SinI GGWCC 6 cut(s) 824, 838, 857, 953, 1430, 2012
SmaI CCCGGG 1 cut(s) 291
SmiMI CAYNNNNRTG 1 cut(s) 891
SmlI CTYRAG 2 cut(s) 1647, 1712
SmoI CTYRAG 2 cut(s) 1647, 1712
SnaBI TACGTA 1 cut(s) 1189
SpeI ACTAGT 1 cut(s) 1138
SsiI CCGC 4 cut(s) 71, 113, 352, 621
SspI AATATT 1 cut(s) 1294
SspMI CTAG 7 cut(s) 582, 801, 1139, 1401, 1617, 1656, 2006
StyI CCWWGG 3 cut(s) 1169, 1755, 1978
TaaI ACNGT 5 cut(s) 523, 874, 1607, 1628, 1704
TaiI ACGT 6 cut(s) 35, 457, 855, 1117, 1191, 1570
TaqI TCGA 6 cut(s) 94, 117, 483, 559, 1150, 1826
TaqII GACCGA 1 cut(s) 627
TatI WGTACW 3 cut(s) 711, 1141, 1518
TauI GCSGC 2 cut(s) 74, 355
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 4 cut(s) 240, 336, 972, 1794
Tru9I TTAA 4 cut(s) 240, 336, 972, 1794
TscAI CASTG 1 cut(s) 1633
TseFI GTSAC 2 cut(s) 406, 1651
TseI GCWGC 5 cut(s) 445, 881, 980, 1199, 1469
Tsp45I GTSAC 2 cut(s) 406, 1651
TspDTI ATGAA 7 cut(s) 53, 367, 533, 583, 1069, 1281, 1862
TspGWI ACGGA 1 cut(s) 362
TspMI CCCGGG 1 cut(s) 289
TspRI CASTG 1 cut(s) 1633
VpaK11BI GGWCC 6 cut(s) 824, 838, 857, 953, 1430, 2012
XagI CCTNNNNNAGG 1 cut(s) 1977
XapI RAATTY 2 cut(s) 7, 1869
XmaI CCCGGG 1 cut(s) 289
XmnI GAANNNNTTC 2 cut(s) 648, 1164
XspI CTAG 7 cut(s) 582, 801, 1139, 1401, 1617, 1656, 2006
ZrmI AGTACT 1 cut(s) 713
Zsp2I ATGCAT 1 cut(s) 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.