AT4G37950

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000523
Physical Location & Seq
Forward (+)
17844361 .. 17849274
4914 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G37950.2

Sequence Viewer

Length: 2037 bp
ATGGAATACGGAGCTCCGGGAATGAAGGCTGGTCGCCGGGTTCTAACTCCACTTTTCGTTCTAGTTTTCCTTCTTCAAGGTGTTCATTCTCAAACATCACGAAACAAATTCAACATCGCAAAAGCGGGACATAAACTTGCATCTCCCCCGGTTACGCTTAAGAAAGTTGGTGATAACCAAGTGGTCGTTGATAACGGTATCATCGATGTTACCTTCTCGAGTCCACAAGGATTAATAACTAGAATCAAGTACAATGGCTTAAACAATGTGTTGAACGACCAAATTGAAAATCGGGGGTACTGGGACGTAGTATGGTACAAACCAGGACAAGTATCGTCAACTGATTATTTAGTAGGAACAAAATTCGATATTGTAAATCAAACAAGTGAACAAATTGAAATTTCATTTTCGAGAACGTTCTCTCAACGGGGCTCAGGAATCCCATTAAACGTAGACAAACGATATATTATTCGAAGAGGTGTATCCGGAATATACATGTACGCTGTATTGGAGAGGTTAATAGGTTGGCCTGACGTGGACATGGACCAAATCAGAATCGTCTTCAAGCTTAACACAACAAAATTCGATTTCATGGCTGTTTCCGATAATCGGCAAAAAATAATGCCTTTTGATACAGATCGAGACATTACCAAAGGTCGTGCCAGTCCATTGGCTTACAAAGAAGCTGTTCATTTGATAAATCCACAAAATCATATGCTTAAAGGGCAGGTAGACGATAAGTACATGTACTCGGTGGAAAACAAAGACAACAAAGTACACGGTTGGATATCGTCGGACCAACGTATCGGTTTCTGGATGATCACGCCCAGCGACGAGTTCCATGCATGTGGGCCGATCAAACAAGATCTCACTTCTCATGTGGGCCCCACCACTCTTTCGATGTTTACAAGTGTACATTACGCGGGAAAAGATATGAACACAAATTATAAAAGCAAGGAACCGTGGAAGAAGGTGTTTGGTCCTGTCTTTGTTTATCTCAATTCTGCTTCTTCTCGTAATCTTCTCTGGACCGATGCTAAACGACAGATGGTTTCCGAGGTCCAAAGCTGGCCTTATGATTTCGTGAAATCTGTAGATTACCCTCTTCATCACCAAAGAGGAACAGTCAAGGGTCAATTATTTGTCATAGACAGATACATCAAAAACGTGACGTACTTATTCGGGCAATTTGCTTTTGTGGGATTAGCATTACCTGGTGAAGCTGGTTCTTGGCAAACCGAAAACAAGGGATATCAATTTTGGACACGAGCAGACAAAATGGGAATGTTCACAATAGCGAATGTGAGACCAGGGACGTATAGTCTCTACGCATGGGTTTCTGGATTTATCGGTGACTATAAATATGTGCGTGACATTACCATCACACCAGGTAGGGAGATAGACGTTGGACACATAGTGTACGTGCCACCGAGAAACGGCCCAACACTGTGGGAGATCGGACAACCGGACCGAACCGCGGCAGAATTCTATATCCCAGATCCGGACCCAACCCTTTTCACCAAACTTTATCTAAATTACTCAAATCCTCAAGATAGATTTAGACAGTATGGTCTATGGGATCGTTACAGTGTTTTGTATCCTCGAAACGATCTTGTTTTTACTGCTGGAGTGAGTGACTATAAAAAAGACTGGTTCTATGCCCATGTCAACAGGAAAGCTGGAAATGGGACATATAAAGCAACGACATGGCAAATTAAGTTTAATTTGAAAGCAGTGATTCAAACTAGAATATACACACTTCGAATAGCTTTGGCTGCAGCCTCAACAATTGATCTACTGGTTTGGGTCAACGAAGTTGATTCCAAACCTCTATTCATAACTGGGCTAATCGGTCGGGATAACGCGATCGCGAGGCATGGGATTCATGGGTTGTACAAATTGTACAATATCGATGTTCATGGAAAGTTACTTAGGGTTGGTAATAATACTATTTTTCTTACTCATGGTCGGAACTCTGATTCTTTTTCAGGTGTAATGTATGATTATCTTCGTTTGGAAGGTCCTTCCGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

678

Amino Acids

77.33

Weight (kDa)

9.4

Isoelectric Point (pI)

29.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 33 - 244 1.4e-104 Rhamnogalacturonate lyase family
fn3_3 PF14686 397 - 468 1.8e-25 Polysaccharide lyase family 4, domain II
CBM-like PF14683 482 - 672 3.2e-46 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 948
AasI GACNNNNNNGTC 1 cut(s) 1569
Acc36I ACCTGC 1 cut(s) 718
AccI GTMKAC 2 cut(s) 453, 732
AccII CGCG 4 cut(s) 923, 1478, 1865, 1871
AccIII TCCGGA 3 cut(s) 485, 1501, 2027
AciI CCGC 4 cut(s) 125, 923, 1476, 1478
AclI AACGTT 1 cut(s) 416
AclWI GGATC 2 cut(s) 1493, 1587
AcsI RAATTY 5 cut(s) 107, 362, 399, 581, 1484
AdeI CACNNNGTG 1 cut(s) 1417
AfiI CCNNNNNNNGG 4 cut(s) 609, 1436, 1477, 1501
AflII CTTAAG 1 cut(s) 158
AflIII ACRYGT 2 cut(s) 495, 744
AgsI TTSAA 8 cut(s) 77, 112, 274, 287, 398, 565, 1729, 1742
AhdI GACNNNNNGTC 1 cut(s) 1320
AjiI CACGTC 1 cut(s) 535
AjnI CCWGG 4 cut(s) 322, 1213, 1309, 1387
AjuI GAANNNNNNNTTGG 2 cut(s) 1817, 1849
AluBI AGCT 7 cut(s) 14, 568, 686, 1068, 1223, 1679, 1769
AluI AGCT 7 cut(s) 14, 568, 686, 1068, 1223, 1679, 1769
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 3 cut(s) 636, 1300, 1328
AlwI GGATC 2 cut(s) 1493, 1587
Ama87I CYCGRG 1 cut(s) 217
Aor13HI TCCGGA 3 cut(s) 485, 1501, 2027
AoxI GGCC 5 cut(s) 527, 851, 883, 1070, 1438
ApaI GGGCCC 1 cut(s) 887
ApeKI GCWGC 2 cut(s) 1775, 1778
ApoI RAATTY 5 cut(s) 107, 362, 399, 581, 1484
AseI ATTAAT 1 cut(s) 233
AsiSI GCGATCGC 1 cut(s) 1869
Asp700I GAANNNNTTC 1 cut(s) 687
AsuC2I CCSGG 3 cut(s) 18, 38, 149
AsuHPI GGTGA 5 cut(s) 182, 1103, 1229, 1364, 1510
AsuII TTCGAA 2 cut(s) 472, 1762
AvaI CYCGRG 1 cut(s) 217
AvaII GGWCC 8 cut(s) 544, 796, 980, 1029, 1060, 1468, 1504, 2021
BaeGI GKGCMC 1 cut(s) 887
BaeI ACNNNNGTAYC 2 cut(s) 787, 820
BanII GRGCYC 3 cut(s) 16, 434, 887
BauI CACGAG 1 cut(s) 1266
BbsI GAAGAC 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 2 cut(s) 1762, 1790
BccI CCATC 2 cut(s) 1042, 1388
BceAI ACGGC 1 cut(s) 1453
BcgI CGANNNNNNTGC 2 cut(s) 824, 858
BciT130I CCWGG 4 cut(s) 324, 1215, 1311, 1389
BciVI GTATCC 2 cut(s) 493, 1608
BclI TGATCA 1 cut(s) 819
BcnI CCSGG 3 cut(s) 18, 38, 149
BcoDI GTCTC 3 cut(s) 636, 1300, 1328
BfaI CTAG 3 cut(s) 62, 240, 1746
BfmI CTRYAG 2 cut(s) 1092, 1776
BfrI CTTAAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 718
BfuI GTATCC 2 cut(s) 493, 1608
BglII AGATCT 1 cut(s) 865
BisI GCNGC 3 cut(s) 1479, 1776, 1779
BlsI GCNGC 3 cut(s) 1480, 1777, 1780
Bme1390I CCNGG 7 cut(s) 18, 38, 149, 324, 1215, 1311, 1389
Bme18I GGWCC 8 cut(s) 544, 796, 980, 1029, 1060, 1468, 1504, 2021
BmeRI GACNNNNNGTC 1 cut(s) 1320
BmeT110I CYCGRG 1 cut(s) 217
BmgBI CACGTC 1 cut(s) 535
BmiI GGNNCC 4 cut(s) 885, 886, 960, 1506
BmrFI CCNGG 7 cut(s) 18, 38, 149, 324, 1215, 1311, 1389
BmrI ACTGGG 2 cut(s) 310, 1851
BmsI GCATC 2 cut(s) 149, 1024
BmuI ACTGGG 2 cut(s) 310, 1851
BpiI GAAGAC 1 cut(s) 553
BpmI CTGGAG 1 cut(s) 1647
Bpu10I CCTNAGC 1 cut(s) 433
Bpu14I TTCGAA 2 cut(s) 472, 1762
BpuEI CTTGAG 1 cut(s) 1533
BpuMI CCSGG 3 cut(s) 18, 38, 149
Bsa29I ATCGAT 2 cut(s) 204, 1911
BsaAI YACGTR 1 cut(s) 1423
BsaBI GATNNNNATC 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 1300
BsaJI CCNNGG 5 cut(s) 147, 962, 1056, 1310, 1476
BsaWI WCCGGW 4 cut(s) 485, 1465, 1501, 2027
Bsc4I CCNNNNNNNGG 4 cut(s) 609, 1436, 1477, 1501
Bse1I ACTGG 5 cut(s) 305, 663, 1655, 1803, 1846
Bse8I GATNNNNATC 1 cut(s) 636
BseAI TCCGGA 3 cut(s) 485, 1501, 2027
BseBI CCWGG 4 cut(s) 324, 1215, 1311, 1389
BseCI ATCGAT 2 cut(s) 204, 1911
BseDI CCNNGG 5 cut(s) 147, 962, 1056, 1310, 1476
BseGI GGATG 1 cut(s) 822
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 4 cut(s) 609, 1436, 1477, 1501
BseMII CTCAG 1 cut(s) 447
BseNI ACTGG 5 cut(s) 305, 663, 1655, 1803, 1846
BseSI GKGCMC 1 cut(s) 887
BseXI GCAGC 2 cut(s) 1762, 1790
BseYI CCCAGC 1 cut(s) 827
Bsh1236I CGCG 4 cut(s) 923, 1478, 1865, 1871
Bsh1285I CGRYCG 2 cut(s) 1855, 1869
BshFI GGCC 5 cut(s) 529, 853, 885, 1072, 1440
BshVI ATCGAT 2 cut(s) 204, 1911
BsiEI CGRYCG 2 cut(s) 1855, 1869
BsiHKAI GWGCWC 1 cut(s) 16
BsiHKCI CYCGRG 1 cut(s) 217
BsiSI CCGG 7 cut(s) 17, 37, 149, 486, 1466, 1502, 2028
BslFI GGGAC 4 cut(s) 141, 317, 1327, 1702
BslI CCNNNNNNNGG 4 cut(s) 609, 1436, 1477, 1501
BsmAI GTCTC 3 cut(s) 636, 1300, 1328
BsmFI GGGAC 4 cut(s) 141, 317, 1327, 1702
BsnI GGCC 5 cut(s) 529, 853, 885, 1072, 1440
Bso31I GGTCTC 1 cut(s) 1300
BsoBI CYCGRG 1 cut(s) 217
Bsp119I TTCGAA 2 cut(s) 472, 1762
Bsp120I GGGCCC 1 cut(s) 883
Bsp1286I GDGCHC 3 cut(s) 16, 434, 887
Bsp13I TCCGGA 3 cut(s) 485, 1501, 2027
Bsp1407I TGTACA 3 cut(s) 913, 1893, 1902
Bsp68I TCGCGA 1 cut(s) 1871
BspACI CCGC 4 cut(s) 125, 923, 1476, 1478
BspANI GGCC 5 cut(s) 529, 853, 885, 1072, 1440
BspCNI CTCAG 1 cut(s) 446
BspDI ATCGAT 2 cut(s) 204, 1911
BspEI TCCGGA 3 cut(s) 485, 1501, 2027
BspFNI CGCG 4 cut(s) 923, 1478, 1865, 1871
BspLI GGNNCC 4 cut(s) 885, 886, 960, 1506
BspMAI CTGCAG 1 cut(s) 1780
BspMI ACCTGC 1 cut(s) 718
BspPI GGATC 2 cut(s) 1493, 1587
BspT104I TTCGAA 2 cut(s) 472, 1762
BspTI CTTAAG 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 1300
BsrGI TGTACA 3 cut(s) 913, 1893, 1902
BsrI ACTGG 5 cut(s) 305, 663, 1655, 1803, 1846
BssECI CCNNGG 5 cut(s) 147, 962, 1056, 1310, 1476
BssSI CACGAG 1 cut(s) 1266
Bst2BI CACGAG 1 cut(s) 1266
Bst2UI CCWGG 4 cut(s) 324, 1215, 1311, 1389
Bst4CI ACNGT 7 cut(s) 197, 782, 963, 1126, 1449, 1566, 1589
Bst6I CTCTTC 2 cut(s) 469, 1110
BstAFI CTTAAG 1 cut(s) 158
BstAUI TGTACA 3 cut(s) 913, 1893, 1902
BstBAI YACGTR 1 cut(s) 1423
BstBI TTCGAA 2 cut(s) 472, 1762
BstC8I GCNNGC 1 cut(s) 1070
BstDEI CTNAG 2 cut(s) 433, 1931
BstDSI CCRYGG 2 cut(s) 962, 1476
BstF5I GGATG 1 cut(s) 822
BstFNI CGCG 4 cut(s) 923, 1478, 1865, 1871
BstMAI GTCTC 3 cut(s) 636, 1300, 1328
BstMCI CGRYCG 2 cut(s) 1855, 1869
BstMWI GCNNNNNNNGC 2 cut(s) 724, 1775
BstNI CCWGG 4 cut(s) 324, 1215, 1311, 1389
BstNSI RCATGY 3 cut(s) 499, 748, 849
BstSCI CCNGG 7 cut(s) 16, 36, 147, 322, 1213, 1309, 1387
BstSFI CTRYAG 2 cut(s) 1092, 1776
BstSLI GKGCMC 1 cut(s) 887
BstUI CGCG 4 cut(s) 923, 1478, 1865, 1871
BstV1I GCAGC 2 cut(s) 1762, 1790
BstV2I GAAGAC 1 cut(s) 553
BstX2I RGATCY 2 cut(s) 865, 1498
BstXI CCANNNNNNTGG 3 cut(s) 670, 848, 1449
BstYI RGATCY 2 cut(s) 865, 1498
Bsu15I ATCGAT 2 cut(s) 204, 1911
BsuI GTATCC 2 cut(s) 493, 1608
BsuRI GGCC 5 cut(s) 529, 853, 885, 1072, 1440
BsuTUI ATCGAT 2 cut(s) 204, 1911
BtgI CCRYGG 2 cut(s) 962, 1476
BtgZI GCGATG 1 cut(s) 100
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 1 cut(s) 822
BtsI GCAGTG 1 cut(s) 1740
BtsIMutI CAGTG 3 cut(s) 1445, 1594, 1740
BtuMI TCGCGA 1 cut(s) 1871
BveI ACCTGC 1 cut(s) 718
Cac8I GCNNGC 1 cut(s) 1070
Cfr42I CCGCGG 1 cut(s) 1479
ClaI ATCGAT 2 cut(s) 204, 1911
CpoI CGGWCCG 1 cut(s) 1468
CsiI ACCWGGT 2 cut(s) 1213, 1387
CspI CGGWCCG 1 cut(s) 1468
DdeI CTNAG 2 cut(s) 433, 1931
DraIII CACNNNGTG 1 cut(s) 1417
DrdI GACNNNNNNGTC 1 cut(s) 1569
DriI GACNNNNNGTC 1 cut(s) 1320
DseDI GACNNNNNNGTC 1 cut(s) 1569
Eam1104I CTCTTC 2 cut(s) 469, 1110
Eam1105I GACNNNNNGTC 1 cut(s) 1320
EarI CTCTTC 2 cut(s) 469, 1110
Ecl136II GAGCTC 1 cut(s) 14
Eco24I GRGCYC 3 cut(s) 16, 434, 887
Eco31I GGTCTC 1 cut(s) 1300
Eco32I GATATC 2 cut(s) 789, 1253
Eco47I GGWCC 8 cut(s) 544, 796, 980, 1029, 1060, 1468, 1504, 2021
Eco53kI GAGCTC 1 cut(s) 14
Eco88I CYCGRG 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 14
EcoO109I RGGNCCY 2 cut(s) 884, 2021
EcoRI GAATTC 1 cut(s) 1484
EcoRII CCWGG 4 cut(s) 322, 1213, 1309, 1387
EcoRV GATATC 2 cut(s) 789, 1253
EcoT22I ATGCAT 1 cut(s) 847
EcoT38I GRGCYC 3 cut(s) 16, 434, 887
FalI AAGNNNNNCTT 2 cut(s) 1057, 1089
FaqI GGGAC 4 cut(s) 141, 317, 1327, 1702
FauI CCCGC 2 cut(s) 118, 916
FauNDI CATATG 1 cut(s) 714
FbaI TGATCA 1 cut(s) 819
FblI GTMKAC 2 cut(s) 453, 732
Fnu4HI GCNGC 3 cut(s) 1479, 1776, 1779
FokI GGATG 1 cut(s) 829
FriOI GRGCYC 3 cut(s) 16, 434, 887
Fsp4HI GCNGC 3 cut(s) 1479, 1776, 1779
FspBI CTAG 3 cut(s) 62, 240, 1746
GluI GCNGC 3 cut(s) 1479, 1776, 1779
GsaI CCCAGC 1 cut(s) 831
GsuI CTGGAG 1 cut(s) 1647
HaeIII GGCC 5 cut(s) 529, 853, 885, 1072, 1440
HapII CCGG 7 cut(s) 17, 37, 149, 486, 1466, 1502, 2028
HincII GTYRAC 3 cut(s) 339, 1669, 1810
HindII GTYRAC 3 cut(s) 339, 1669, 1810
HindIII AAGCTT 1 cut(s) 566
HinfI GANTC 8 cut(s) 220, 243, 438, 555, 1738, 1820, 1882, 1979
HpaII CCGG 7 cut(s) 17, 37, 149, 486, 1466, 1502, 2028
HphI GGTGA 5 cut(s) 182, 1103, 1229, 1364, 1510
Hpy188I TCNGA 7 cut(s) 554, 604, 796, 1057, 1460, 1971, 1978
Hpy99I CGWCG 2 cut(s) 796, 836
HpyAV CCTTC 6 cut(s) 19, 80, 223, 964, 2012, 2034
HpyCH4III ACNGT 7 cut(s) 197, 782, 963, 1126, 1449, 1566, 1589
HpyCH4V TGCA 3 cut(s) 140, 845, 1778
HpyF10VI GCNNNNNNNGC 2 cut(s) 724, 1775
HpyF3I CTNAG 2 cut(s) 433, 1931
Kpn2I TCCGGA 3 cut(s) 485, 1501, 2027
Ksp22I TGATCA 1 cut(s) 819
KspI CCGCGG 1 cut(s) 1479
LmnI GCTCC 2 cut(s) 11, 19
Lsp1109I GCAGC 2 cut(s) 1762, 1790
LweI GCATC 2 cut(s) 149, 1024
MabI ACCWGGT 2 cut(s) 1213, 1387
MaeI CTAG 3 cut(s) 62, 240, 1746
MaeIII GTNAC 8 cut(s) 151, 208, 1168, 1352, 1370, 1583, 1634, 1926
MboII GAAGA 8 cut(s) 65, 486, 553, 979, 1002, 1013, 1097, 2000
MfeI CAATTG 1 cut(s) 1788
MflI RGATCY 2 cut(s) 865, 1498
MhlI GDGCHC 3 cut(s) 16, 434, 887
MlyI GAGTC 1 cut(s) 229
MmeI TCCRAC 4 cut(s) 764, 774, 1387, 1949
Mph1103I ATGCAT 1 cut(s) 847
MroI TCCGGA 3 cut(s) 485, 1501, 2027
MroXI GAANNNNTTC 1 cut(s) 687
MseI TTAA 9 cut(s) 159, 233, 260, 446, 518, 570, 720, 1716, 1722
MslI CAYNNNNRTG 1 cut(s) 846
MspA1I CMGCKG 1 cut(s) 1478
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 7 cut(s) 17, 37, 149, 486, 1466, 1502, 2028
MspR9I CCNGG 7 cut(s) 18, 38, 149, 324, 1215, 1311, 1389
MunI CAATTG 1 cut(s) 1788
MvaI CCWGG 4 cut(s) 324, 1215, 1311, 1389
MvnI CGCG 4 cut(s) 923, 1478, 1865, 1871
MwoI GCNNNNNNNGC 2 cut(s) 724, 1775
NciI CCSGG 3 cut(s) 18, 38, 149
NdeI CATATG 1 cut(s) 714
NlaIV GGNNCC 4 cut(s) 885, 886, 960, 1506
NmuCI GTSAC 4 cut(s) 1168, 1352, 1370, 1634
NruI TCGCGA 1 cut(s) 1871
NsiI ATGCAT 1 cut(s) 847
NspI RCATGY 3 cut(s) 499, 748, 849
NspV TTCGAA 2 cut(s) 472, 1762
PaeR7I CTCGAG 1 cut(s) 217
PciI ACATGT 2 cut(s) 495, 744
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 7 cut(s) 243, 438, 555, 1738, 1820, 1882, 1979
PfoI TCCNGGA 1 cut(s) 16
PkrI GCNGC 3 cut(s) 1480, 1777, 1780
Ple19I CGATCG 1 cut(s) 1869
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
Ppu21I YACGTR 1 cut(s) 1423
PpuMI RGGWCCY 1 cut(s) 2021
PscI ACATGT 2 cut(s) 495, 744
PshBI ATTAAT 1 cut(s) 233
PsiI TTATAA 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 16
Psp1406I AACGTT 1 cut(s) 416
Psp5II RGGWCCY 1 cut(s) 2021
Psp6I CCWGG 4 cut(s) 322, 1213, 1309, 1387
PspFI CCCAGC 1 cut(s) 827
PspGI CCWGG 4 cut(s) 322, 1213, 1309, 1387
PspN4I GGNNCC 4 cut(s) 885, 886, 960, 1506
PspOMI GGGCCC 1 cut(s) 883
PspPPI RGGWCCY 1 cut(s) 2021
PstI CTGCAG 1 cut(s) 1780
PsuI RGATCY 2 cut(s) 865, 1498
PvuI CGATCG 1 cut(s) 1869
RgaI GCGATCGC 1 cut(s) 1869
RruI TCGCGA 1 cut(s) 1871
RseI CAYNNNNRTG 1 cut(s) 846
Rsr2I CGGWCCG 1 cut(s) 1468
RsrII CGGWCCG 1 cut(s) 1468
SacI GAGCTC 1 cut(s) 16
SacII CCGCGG 1 cut(s) 1479
SaqAI TTAA 9 cut(s) 159, 233, 260, 446, 518, 570, 720, 1716, 1722
SatI GCNGC 3 cut(s) 1479, 1776, 1779
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 7 cut(s) 18, 38, 149, 324, 1215, 1311, 1389
SduI GDGCHC 3 cut(s) 16, 434, 887
SexAI ACCWGGT 2 cut(s) 1213, 1387
SfaAI GCGATCGC 1 cut(s) 1869
SfaNI GCATC 2 cut(s) 149, 1024
SfcI CTRYAG 2 cut(s) 1092, 1776
Sfr274I CTCGAG 1 cut(s) 217
Sfr303I CCGCGG 1 cut(s) 1479
SfuI TTCGAA 2 cut(s) 472, 1762
SgfI GCGATCGC 1 cut(s) 1869
SgrBI CCGCGG 1 cut(s) 1479
SinI GGWCC 8 cut(s) 544, 796, 980, 1029, 1060, 1468, 1504, 2021
SlaI CTCGAG 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 846
SmlI CTYRAG 3 cut(s) 158, 217, 1548
SmoI CTYRAG 3 cut(s) 158, 217, 1548
SsiI CCGC 4 cut(s) 125, 923, 1476, 1478
SspMI CTAG 3 cut(s) 62, 240, 1746
SstI GAGCTC 1 cut(s) 16
StyD4I CCNGG 7 cut(s) 16, 36, 147, 322, 1213, 1309, 1387
TaaI ACNGT 7 cut(s) 197, 782, 963, 1126, 1449, 1566, 1589
TaqII GACCGA 3 cut(s) 1046, 1485, 1841
TatI WGTACW 7 cut(s) 249, 741, 747, 775, 913, 1893, 1902
TauI GCSGC 1 cut(s) 1481
TfiI GAWTC 7 cut(s) 243, 438, 555, 1738, 1820, 1882, 1979
Tru1I TTAA 9 cut(s) 159, 233, 260, 446, 518, 570, 720, 1716, 1722
Tru9I TTAA 9 cut(s) 159, 233, 260, 446, 518, 570, 720, 1716, 1722
TscAI CASTG 3 cut(s) 1452, 1594, 1740
TseFI GTSAC 4 cut(s) 1168, 1352, 1370, 1634
TseI GCWGC 2 cut(s) 1775, 1778
Tsp45I GTSAC 4 cut(s) 1168, 1352, 1370, 1634
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 3 cut(s) 1452, 1594, 1740
Vha464I CTTAAG 1 cut(s) 158
VpaK11BI GGWCC 8 cut(s) 544, 796, 980, 1029, 1060, 1468, 1504, 2021
VspI ATTAAT 1 cut(s) 233
XapI RAATTY 5 cut(s) 107, 362, 399, 581, 1484
XceI RCATGY 3 cut(s) 499, 748, 849
XhoI CTCGAG 1 cut(s) 217
XmiI GTMKAC 2 cut(s) 453, 732
XmnI GAANNNNTTC 1 cut(s) 687
XspI CTAG 3 cut(s) 62, 240, 1746
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.