pycom15g27240

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000523
Physical Location & Seq
Forward (+)
22574587 .. 22578277
3691 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g27240.2

Sequence Viewer

Length: 1953 bp
ATGAAATATTTACTGTGTAATTGCAGAAGAACACTAAGGAGCGCTACTGGATCAGATACTTGGTACAACAACATGCTAACAAAGACTAACAACCTGGTAGTACTGAATAATGGCATTGTCCGGCTTACATTTTCTCATCCCGGAGGAGACGTCATTGGAATCCAGTACAAGGGAATCGACAACTTGCTTGAAATCAAGAATCATGAAAATAATAGAGGGTATTGGGATGTTGTCTGGAATAACCGAGAGACAGAAGGTGTGGACAAACTGCAAGGGACAAAATTTAAAGTTATCACAGCGAGGGCCGATCAAATAGAAATTTCTTTTACTAAAACCTACGATGTTTCTCTCGGTAATGCCACACTTTCCATGAATGTTGACAAAAGGTACATAATGCAGCGTGGTCGCGCCGGGTTCTATGCATATTCCATATTTGAGCGTCCTGAAGGGTGGCCAGAAATGGACATTGATCAAATTAGAATTGTTTACAAACTTCAACAAGACAAATTTCAGTTTATGGTTGTATCTGACGACAGACAAAGGATCATGCCAACAGCCGAGGACCGTGAAAACGGTATTAAACTTGCGTATCCTGAAGCTGTTCTTTTGACCAATCCAAGCAATCCTGACCTTAGAGGAGAGGTGGACGATAAGTACCAATACTCAAGCGAGAACAAAGATAACAAGGTCCATGGATGGATTTGCACTAACCCAGCTGTAGGCTTTTGGATTATCATACCTAATGATGAGTTTCGCACAGCTGGGCCTTTCAAGCAGGAGCTCACCTCTCATGTAGGCCCAACCGCCCTCTCTATGTTTGTTAGTACTCACTATGCTGGGAAGGAAGTTGGGATGAGATTTACAGAAGGTGAGGCATGGAAGAAAGTTTTTGGGCCTGTCTTTGTGTATCTCAATTCAGTTCCGACCTTAAATGAAACTATTCTTTGGGAAAATGCTAAAGAGCAGTTGGCTGAAGAAGTCAATAGCTGGCCATACAATTTCACTCAATCAGAAAACTTCCCTAGCTCAAGCGGACGCGGATCAGTTGCCGGTCAATTACTAGTACAGGATTGGTACATCAGCAAGAGCCATGTCTGGGCAAATTCCGCATATGTAGGATTGGCTCCACCTGGAAATGCGGGATCATGGCAAAAAGAAAGCAAGGGTTATCAGTTCTGGACTCAAGCCAATGAGCAAGGTTATTTCCTTATTAGAGATGCTCGACCTGGAAATTATACTTTGTATGCAACTGTTCCCGGCATTATTGGGGATTACAAATATGAGGCTAATATTACAATTGAACCAGGAAGTGAAATCAATTTGGCTAATCTTACCTACGTACCTCCAAGAAATGGTCCCACCCTATGGGAAATTGGCATACCAGATCGATCCGCGGCTGAGTTTAACGTACCCGATCCAAATCCCACCCTTATGAACAAATTGTTCACTACCAATCGTGCAAACAAGTTTAGGCAATATGGCTTGTGGGAACGATATGCAGATTTATACCCCGATGAGGATCTCATCTATACCGTTGGTACTGATAATTACAGTGATAATTGGTTCTTCGCTCATGTGACAAGGAACATCGGAAATCATACATATGAACCAACAACATGGAAAATTCTATTTGAACTTCAAAATTTGACAAATCCAGGAAATTATACACTCCAGTTAGCATTGGCCTCAGCCACTAATGCAGAAGTTCAGGTTCGATTCAACAACCAGAGCGCCTTCCCACCTCATTTTACGACAGGGCTAATTGGGAGGGACAATGCCATAGCTAGGCATGGAATTCATGGTTTGTACTGGTTGTTCGGTATTATTGTACCGAGCTATCAACTACGAGAAGGAAACAACACCATCTATCTTACCCAGTCAAGAAGCTTGGGCACTTTCGAAGGAGTGATGTACGACTATATCCGACTAGAAGGACCCCCTCCTCGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

651

Amino Acids

74.14

Weight (kDa)

5.78

Isoelectric Point (pI)

29.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 153
AccB7I CCANNNNNTGG 2 cut(s) 1352, 1365
AccII CGCG 3 cut(s) 408, 1038, 1394
AciI CCGC 7 cut(s) 804, 1032, 1038, 1107, 1139, 1392, 1394
AclWI GGATC 7 cut(s) 58, 551, 1048, 1150, 1383, 1409, 1527
AcoI YGGCCR 2 cut(s) 452, 989
AcsI RAATTY 7 cut(s) 281, 318, 506, 1102, 1623, 1642, 1794
AcuI CTGAAG 3 cut(s) 465, 615, 993
AcyI GRCGYC 1 cut(s) 150
AfeI AGCGCT 1 cut(s) 43
AfiI CCNNNNNNNGG 7 cut(s) 169, 719, 1096, 1352, 1365, 1516, 1785
AgsI TTSAA 7 cut(s) 191, 497, 772, 1301, 1634, 1640, 1720
AhlI ACTAGT 1 cut(s) 1060
AjnI CCWGG 5 cut(s) 93, 1129, 1225, 1303, 1654
AluBI AGCT 9 cut(s) 599, 716, 761, 781, 987, 1026, 1784, 1836, 1887
AluI AGCT 9 cut(s) 599, 716, 761, 781, 987, 1026, 1784, 1836, 1887
Alw21I GWGCWC 1 cut(s) 783
Alw26I GTCTC 2 cut(s) 141, 242
AlwI GGATC 7 cut(s) 58, 551, 1048, 1150, 1383, 1409, 1527
Aor51HI AGCGCT 1 cut(s) 43
AoxI GGCC 7 cut(s) 303, 452, 764, 796, 893, 989, 1683
ApeKI GCWGC 1 cut(s) 397
ApoI RAATTY 7 cut(s) 281, 318, 506, 1102, 1623, 1642, 1794
Asp700I GAANNNNTTC 2 cut(s) 600, 939
AspLEI GCGC 3 cut(s) 44, 410, 1733
AspS9I GGNCC 8 cut(s) 303, 562, 688, 764, 797, 893, 1355, 1934
AsuC2I CCSGG 3 cut(s) 141, 412, 1257
AsuHPI GGTGA 2 cut(s) 775, 881
AsuII TTCGAA 1 cut(s) 1899
AvaII GGWCC 4 cut(s) 562, 688, 1355, 1934
BaeGI GKGCMC 1 cut(s) 1895
BaeI ACNNNNGTAYC 4 cut(s) 572, 605, 1323, 1356
BalI TGGCCA 2 cut(s) 454, 991
BanII GRGCYC 1 cut(s) 783
Bbv12I GWGCWC 1 cut(s) 783
BbvCI CCTCAGC 1 cut(s) 1687
BbvI GCAGC 1 cut(s) 409
BccI CCATC 2 cut(s) 690, 1871
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 5 cut(s) 95, 1131, 1227, 1305, 1656
BciVI GTATCC 1 cut(s) 600
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 3 cut(s) 141, 412, 1257
BcoDI GTCTC 2 cut(s) 141, 242
BcuI ACTAGT 1 cut(s) 1060
BfaI CTAG 4 cut(s) 1023, 1061, 1785, 1928
BfmI CTRYAG 1 cut(s) 717
BfoI RGCGCY 2 cut(s) 45, 1734
BfuI GTATCC 1 cut(s) 600
BisI GCNGC 2 cut(s) 398, 1395
BlsI GCNGC 2 cut(s) 399, 1396
BmcAI AGTACT 2 cut(s) 102, 826
Bme1390I CCNGG 8 cut(s) 95, 141, 412, 1131, 1227, 1257, 1305, 1656
Bme18I GGWCC 4 cut(s) 562, 688, 1355, 1934
BmgT120I GGNCC 8 cut(s) 303, 562, 688, 764, 797, 893, 1355, 1934
BmiI GGNNCC 3 cut(s) 1125, 1357, 1936
BmrFI CCNGG 8 cut(s) 95, 141, 412, 1131, 1227, 1257, 1305, 1656
BmrI ACTGGG 1 cut(s) 1870
BmsI GCATC 1 cut(s) 1207
BmuI ACTGGG 1 cut(s) 1870
BplI GAGNNNNNCTC 2 cut(s) 770, 802
BpmI CTGGAG 1 cut(s) 1655
Bpu10I CCTNAGC 1 cut(s) 1687
Bpu14I TTCGAA 1 cut(s) 1899
BpuEI CTTGAG 3 cut(s) 649, 1012, 1167
BpuMI CCSGG 3 cut(s) 141, 412, 1257
Bsa29I ATCGAT 1 cut(s) 1387
BsaAI YACGTR 1 cut(s) 1339
BsaBI GATNNNNATC 2 cut(s) 1419, 1518
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 3 cut(s) 558, 691, 1392
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 7 cut(s) 169, 719, 1096, 1352, 1365, 1516, 1785
Bse118I RCCGGY 1 cut(s) 1049
Bse1I ACTGG 5 cut(s) 52, 163, 1672, 1814, 1876
Bse8I GATNNNNATC 2 cut(s) 1419, 1518
BseBI CCWGG 5 cut(s) 95, 1131, 1227, 1305, 1656
BseCI ATCGAT 1 cut(s) 1387
BseDI CCNNGG 3 cut(s) 558, 691, 1392
BseGI GGATG 4 cut(s) 136, 232, 701, 858
BseJI GATNNNNATC 2 cut(s) 1419, 1518
BseLI CCNNNNNNNGG 7 cut(s) 169, 719, 1096, 1352, 1365, 1516, 1785
BseMII CTCAG 2 cut(s) 1389, 1701
BseNI ACTGG 5 cut(s) 52, 163, 1672, 1814, 1876
BseRI GAGGAG 3 cut(s) 159, 651, 1932
BseSI GKGCMC 1 cut(s) 1895
BseXI GCAGC 1 cut(s) 409
BseYI CCCAGC 3 cut(s) 712, 761, 836
Bsh1236I CGCG 3 cut(s) 408, 1038, 1394
BshFI GGCC 7 cut(s) 305, 454, 766, 798, 895, 991, 1685
BshVI ATCGAT 1 cut(s) 1387
BsiHKAI GWGCWC 1 cut(s) 783
BsiSI CCGG 5 cut(s) 121, 141, 411, 1050, 1257
BslFI GGGAC 3 cut(s) 289, 1341, 1784
BslI CCNNNNNNNGG 7 cut(s) 169, 719, 1096, 1352, 1365, 1516, 1785
BsmAI GTCTC 2 cut(s) 141, 242
BsmBI CGTCTC 1 cut(s) 141
BsmFI GGGAC 3 cut(s) 289, 1341, 1784
BsnI GGCC 7 cut(s) 305, 454, 766, 798, 895, 991, 1685
Bsp119I TTCGAA 1 cut(s) 1899
Bsp1286I GDGCHC 2 cut(s) 783, 1895
Bsp19I CCATGG 1 cut(s) 691
BspACI CCGC 7 cut(s) 804, 1032, 1038, 1107, 1139, 1392, 1394
BspANI GGCC 7 cut(s) 305, 454, 766, 798, 895, 991, 1685
BspCNI CTCAG 2 cut(s) 1390, 1700
BspDI ATCGAT 1 cut(s) 1387
BspFNI CGCG 3 cut(s) 408, 1038, 1394
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 1125, 1357, 1936
BspPI GGATC 7 cut(s) 58, 551, 1048, 1150, 1383, 1409, 1527
BspT104I TTCGAA 1 cut(s) 1899
BsrFI RCCGGY 1 cut(s) 1049
BsrI ACTGG 5 cut(s) 52, 163, 1672, 1814, 1876
BssAI RCCGGY 1 cut(s) 1049
BssECI CCNNGG 3 cut(s) 558, 691, 1392
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 691
Bst2UI CCWGG 5 cut(s) 95, 1131, 1227, 1305, 1656
Bst4CI ACNGT 6 cut(s) 15, 566, 575, 1252, 1534, 1553
BstACI GRCGYC 1 cut(s) 150
BstBAI YACGTR 1 cut(s) 1339
BstBI TTCGAA 1 cut(s) 1899
BstC8I GCNNGC 1 cut(s) 989
BstDEI CTNAG 4 cut(s) 35, 632, 1398, 1687
BstDSI CCRYGG 2 cut(s) 691, 1392
BstF5I GGATG 4 cut(s) 136, 232, 701, 858
BstFNI CGCG 3 cut(s) 408, 1038, 1394
BstH2I RGCGCY 2 cut(s) 45, 1734
BstHHI GCGC 3 cut(s) 44, 410, 1733
BstMAI GTCTC 2 cut(s) 141, 242
BstMWI GCNNNNNNNGC 3 cut(s) 772, 1106, 1697
BstNI CCWGG 5 cut(s) 95, 1131, 1227, 1305, 1656
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 8 cut(s) 93, 139, 410, 1129, 1225, 1255, 1303, 1654
BstSFI CTRYAG 1 cut(s) 717
BstSLI GKGCMC 1 cut(s) 1895
BstSNI TACGTA 1 cut(s) 1339
BstUI CGCG 3 cut(s) 408, 1038, 1394
BstV1I GCAGC 1 cut(s) 409
BstX2I RGATCY 1 cut(s) 1519
BstXI CCANNNNNNTGG 1 cut(s) 1617
BstYI RGATCY 1 cut(s) 1519
Bsu15I ATCGAT 1 cut(s) 1387
BsuI GTATCC 1 cut(s) 600
BsuRI GGCC 7 cut(s) 305, 454, 766, 798, 895, 991, 1685
BsuTUI ATCGAT 1 cut(s) 1387
BtgI CCRYGG 2 cut(s) 691, 1392
BtsCI GGATG 4 cut(s) 136, 232, 701, 858
BtsIMutI CAGTG 1 cut(s) 1558
Cac8I GCNNGC 1 cut(s) 989
CciI TCATGA 1 cut(s) 202
CfoI GCGC 3 cut(s) 44, 410, 1733
Cfr10I RCCGGY 1 cut(s) 1049
Cfr13I GGNCC 8 cut(s) 303, 562, 688, 764, 797, 893, 1355, 1934
Cfr42I CCGCGG 1 cut(s) 1395
ClaI ATCGAT 1 cut(s) 1387
CseI GACGC 2 cut(s) 428, 1044
CsiI ACCWGGT 1 cut(s) 93
DdeI CTNAG 4 cut(s) 35, 632, 1398, 1687
DraI TTTAAA 1 cut(s) 286
EaeI YGGCCR 2 cut(s) 452, 989
Ecl136II GAGCTC 1 cut(s) 781
Eco105I TACGTA 1 cut(s) 1339
Eco130I CCWWGG 1 cut(s) 691
Eco24I GRGCYC 1 cut(s) 783
Eco47I GGWCC 4 cut(s) 562, 688, 1355, 1934
Eco47III AGCGCT 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 3 cut(s) 465, 615, 993
EcoICRI GAGCTC 1 cut(s) 781
EcoO109I RGGNCCY 1 cut(s) 1934
EcoRI GAATTC 1 cut(s) 1794
EcoRII CCWGG 5 cut(s) 93, 1129, 1225, 1303, 1654
EcoT14I CCWWGG 1 cut(s) 691
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 691
Esp3I CGTCTC 1 cut(s) 141
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 3 cut(s) 289, 1341, 1784
FauI CCCGC 1 cut(s) 1132
FauNDI CATATG 2 cut(s) 1111, 1603
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 398, 1395
FokI GGATG 4 cut(s) 123, 239, 708, 865
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 2 cut(s) 398, 1395
FspBI CTAG 4 cut(s) 1023, 1061, 1785, 1928
GlaI GCGC 3 cut(s) 43, 409, 1732
GluI GCNGC 2 cut(s) 398, 1395
GsaI CCCAGC 3 cut(s) 716, 765, 840
GsuI CTGGAG 1 cut(s) 1655
HaeII RGCGCY 2 cut(s) 45, 1734
HaeIII GGCC 7 cut(s) 305, 454, 766, 798, 895, 991, 1685
HapII CCGG 5 cut(s) 121, 141, 411, 1050, 1257
HgaI GACGC 2 cut(s) 428, 1044
HhaI GCGC 3 cut(s) 44, 410, 1733
Hin1I GRCGYC 1 cut(s) 150
Hin6I GCGC 3 cut(s) 42, 408, 1731
HinP1I GCGC 3 cut(s) 42, 408, 1731
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HindIII AAGCTT 1 cut(s) 1885
HinfI GANTC 5 cut(s) 159, 174, 199, 1180, 1716
HpaII CCGG 5 cut(s) 121, 141, 411, 1050, 1257
HphI GGTGA 2 cut(s) 775, 881
Hpy166II GTNNAC 5 cut(s) 262, 379, 487, 646, 1446
Hpy188I TCNGA 6 cut(s) 55, 529, 924, 1012, 1592, 1925
Hpy188III TCNNGA 8 cut(s) 196, 203, 235, 443, 593, 626, 1177, 1881
Hpy8I GTNNAC 5 cut(s) 262, 379, 487, 646, 1446
HpyAV CCTTC 8 cut(s) 248, 440, 835, 860, 1744, 1844, 1895, 1925
HpyCH4III ACNGT 6 cut(s) 15, 566, 575, 1252, 1534, 1553
HpyCH4IV ACGT 3 cut(s) 150, 1338, 1407
HpyCH4V TGCA 9 cut(s) 24, 271, 397, 422, 705, 1247, 1460, 1499, 1700
HpyF10VI GCNNNNNNNGC 3 cut(s) 772, 1106, 1697
HpyF3I CTNAG 4 cut(s) 35, 632, 1398, 1687
HpySE526I ACGT 3 cut(s) 150, 1338, 1407
Hsp92I GRCGYC 1 cut(s) 150
HspAI GCGC 3 cut(s) 42, 408, 1731
Ksp22I TGATCA 1 cut(s) 469
KspI CCGCGG 1 cut(s) 1395
LmnI GCTCC 3 cut(s) 39, 778, 1129
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 1 cut(s) 1207
MabI ACCWGGT 1 cut(s) 93
MaeI CTAG 4 cut(s) 1023, 1061, 1785, 1928
MaeII ACGT 3 cut(s) 150, 1338, 1407
MaeIII GTNAC 1 cut(s) 1576
MboII GAAGA 4 cut(s) 39, 892, 986, 1558
MfeI CAATTG 1 cut(s) 1296
MflI RGATCY 1 cut(s) 1519
MhlI GDGCHC 2 cut(s) 783, 1895
MlsI TGGCCA 2 cut(s) 454, 991
MluNI TGGCCA 2 cut(s) 454, 991
MlyI GAGTC 1 cut(s) 1174
MmeI TCCRAC 2 cut(s) 947, 1948
Mox20I TGGCCA 2 cut(s) 454, 991
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 2 cut(s) 600, 939
MscI TGGCCA 2 cut(s) 454, 991
MseI TTAA 4 cut(s) 285, 579, 929, 1404
MslI CAYNNNNRTG 2 cut(s) 1430, 1602
Msp20I TGGCCA 2 cut(s) 454, 991
MspA1I CMGCKG 3 cut(s) 716, 761, 1394
MspI CCGG 5 cut(s) 121, 141, 411, 1050, 1257
MspR9I CCNGG 8 cut(s) 95, 141, 412, 1131, 1227, 1257, 1305, 1656
MunI CAATTG 1 cut(s) 1296
MvaI CCWGG 5 cut(s) 95, 1131, 1227, 1305, 1656
MvnI CGCG 3 cut(s) 408, 1038, 1394
MwoI GCNNNNNNNGC 3 cut(s) 772, 1106, 1697
NciI CCSGG 3 cut(s) 141, 412, 1257
NcoI CCATGG 1 cut(s) 691
NdeI CATATG 2 cut(s) 1111, 1603
NlaIV GGNNCC 3 cut(s) 1125, 1357, 1936
NmeAIII GCCGAG 1 cut(s) 583
NmuCI GTSAC 1 cut(s) 1576
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 76
NspV TTCGAA 1 cut(s) 1899
PagI TCATGA 1 cut(s) 202
PdmI GAANNNNTTC 2 cut(s) 600, 939
PfeI GAWTC 4 cut(s) 159, 174, 199, 1716
PflMI CCANNNNNTGG 2 cut(s) 1352, 1365
PfoI TCCNGGA 2 cut(s) 139, 1654
PkrI GCNGC 2 cut(s) 399, 1396
PleI GAGTC 1 cut(s) 1174
PpsI GAGTC 1 cut(s) 1174
Ppu21I YACGTR 1 cut(s) 1339
PpuMI RGGWCCY 1 cut(s) 1934
Psp124BI GAGCTC 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 1934
Psp6I CCWGG 5 cut(s) 93, 1129, 1225, 1303, 1654
PspFI CCCAGC 3 cut(s) 712, 761, 836
PspGI CCWGG 5 cut(s) 93, 1129, 1225, 1303, 1654
PspN4I GGNNCC 3 cut(s) 1125, 1357, 1936
PspPI GGNCC 8 cut(s) 303, 562, 688, 764, 797, 893, 1355, 1934
PspPPI RGGWCCY 1 cut(s) 1934
PsrI GAACNNNNNNTAC 2 cut(s) 1799, 1831
PsuI RGATCY 1 cut(s) 1519
PvuII CAGCTG 2 cut(s) 716, 761
RseI CAYNNNNRTG 2 cut(s) 1430, 1602
SacI GAGCTC 1 cut(s) 783
SacII CCGCGG 1 cut(s) 1395
SaqAI TTAA 4 cut(s) 285, 579, 929, 1404
SatI GCNGC 2 cut(s) 398, 1395
Sau96I GGNCC 8 cut(s) 303, 562, 688, 764, 797, 893, 1355, 1934
ScaI AGTACT 2 cut(s) 102, 826
SchI GAGTC 1 cut(s) 1174
ScrFI CCNGG 8 cut(s) 95, 141, 412, 1131, 1227, 1257, 1305, 1656
SduI GDGCHC 2 cut(s) 783, 1895
SexAI ACCWGGT 1 cut(s) 93
SfaNI GCATC 1 cut(s) 1207
SfcI CTRYAG 1 cut(s) 717
Sfr303I CCGCGG 1 cut(s) 1395
SfuI TTCGAA 1 cut(s) 1899
SgrBI CCGCGG 1 cut(s) 1395
SinI GGWCC 4 cut(s) 562, 688, 1355, 1934
SmiMI CAYNNNNRTG 2 cut(s) 1430, 1602
SmlI CTYRAG 3 cut(s) 664, 1027, 1182
SmoI CTYRAG 3 cut(s) 664, 1027, 1182
SnaBI TACGTA 1 cut(s) 1339
SpeI ACTAGT 1 cut(s) 1060
SsiI CCGC 7 cut(s) 804, 1032, 1038, 1107, 1139, 1392, 1394
SspI AATATT 2 cut(s) 8, 1291
SspMI CTAG 4 cut(s) 1023, 1061, 1785, 1928
SstI GAGCTC 1 cut(s) 783
StyD4I CCNGG 8 cut(s) 93, 139, 410, 1129, 1225, 1255, 1303, 1654
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 6 cut(s) 15, 566, 575, 1252, 1534, 1553
TaiI ACGT 3 cut(s) 153, 1341, 1410
TaqI TCGA 6 cut(s) 177, 1222, 1387, 1714, 1899, 1945
TatI WGTACW 5 cut(s) 100, 165, 824, 1063, 1806
TauI GCSGC 1 cut(s) 1397
TfiI GAWTC 4 cut(s) 159, 174, 199, 1716
Tru1I TTAA 4 cut(s) 285, 579, 929, 1404
Tru9I TTAA 4 cut(s) 285, 579, 929, 1404
TscAI CASTG 1 cut(s) 1558
TseFI GTSAC 1 cut(s) 1576
TseI GCWGC 1 cut(s) 397
Tsp45I GTSAC 1 cut(s) 1576
TspDTI ATGAA 7 cut(s) 17, 219, 386, 948, 1448, 1620, 1787
TspRI CASTG 1 cut(s) 1558
Van91I CCANNNNNTGG 2 cut(s) 1352, 1365
VpaK11BI GGWCC 4 cut(s) 562, 688, 1355, 1934
XapI RAATTY 7 cut(s) 281, 318, 506, 1102, 1623, 1642, 1794
XceI RCATGY 1 cut(s) 76
XmnI GAANNNNTTC 2 cut(s) 600, 939
XspI CTAG 4 cut(s) 1023, 1061, 1785, 1928
ZraI GACGTC 1 cut(s) 151
ZrmI AGTACT 2 cut(s) 102, 826
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.