Rorug02G0167000

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000523
Physical Location & Seq
Forward (+)
14978302 .. 14981999
3698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0167000.1

Sequence Viewer

Length: 2562 bp
ATGGCCAATCCAGTGGATGTTTTCGCCGGGGCTGGTGGAGGATTACTATTCAATGTGCTCTATGATGGCCTCAAGGAGCTGACCACCAAGTATGTGCTGTTCAAACACGTCCTCAAAAGCGTCAAATCCACACTCGAATCTCTGGAGCAAGTGGTTCAAGACATAGCACGACTTAACGAGGATCTGGACCGCCCGGAGGAAGAACTAGCGAGTCTAGAAACCCAACTAGAGAAGGGCAAAGTGCTCGTTGCCAAGTGCGGCAAGGTTCGTGTGTGGAGTGTGTACAAGAAGTACAAGTACTCCAGGAAACTTCTTGGATGGAATGACAGTCTTAAAGAACAGTTGGAGATGCTCAAAGTTCATGCAATAAGAGATGGGAAGGAGACCCTGACAACGGTGAAGAGAACAGAGAAGACTGTGAGCCAAATCGAATCGACCAGTTTCAATATAAAGGAGGGAGTGGAGCAAATTGAGACCAACACTGCAAATCTACAGGTGGTGGTCAGCCGAGTGGAAGCGAGAGTTTCGAATATAGAGGCTGGGATAAAGCAGATACAACCCGAAGCTAAAATTGAAGCTTGGTGTGCAGTACCTGAACTTCCAACTCTTACAGTTGGATTGGATGGGCCTTTGAGCGAACTGAAGATGAAGTTGTTCAACAATGGAGTGTCAATGGTTGTGCTGACCGCTCCCGGAGGATGTGGAAAAACCACTTTGGCAACAAAATTCTGTCAAGATGAGCAAGTCCAAGAGAAATTCAAGAACAATATTTTTTTTGTCACTGTTTCCAAAACGCCAAACTTCTACCAAATTGTGCAAGAGCTATACCGAAGCAAGGGTAGCCAGACACCTACTTTTAAAAATGACGAAAATGCACGCACGTGGCTGCAAAAATTTCTGAGGGAAGAAGGACAAAACCCTTTACTGTTGGTCTTGGATGATGTTTGGTCTGGATCAGACTCTTTACTTGCAAAGTTTGAATTCGAAATGCAAGATTACAAAGTTTTAGTGACATCAAGATCTGAATTTCCGAGATATGGCTCTTATGCTTTGCAGTCATTGGATTTTGACAATGCAATGGAACTCTTTCGTCATGCAGCATCCTTGGGAGAAATAAGTTTGCATATTCCACCAGATATTTCAAGACAGGTAGTAGAGGGCTGTAAGGGATTTCCACTTGCTATAACAGTGGCCGGAAAATCACTTTGCGGACAGCCTATAGAGATTTGGCAGAAAAGACTCATCAAATGGTCCGAACATTCTTCGATTCTTGATTTCGAGACTCAATTGCTTCTTTGCCTCCAAACCAGTTTAGATGTCTCGAATAAGGAAATGAGTATGGTCAAAGAATGTTTCTTAGACCTAGGTTCATTTCCTGAAGATCAAAGAATCCCTGTTGCTGCCCTCATTGATATCTGGACTGAGTCTTACGGTTCACTAGATGAAGACATTTTGTGTGTTGAAAACCTCCACCAGATCACCAACCGAAGTCTAGCTAATCTTGTAGTCACAAGGAAGGATCAGCTGCAGGGGGATGGATACTACAGTGAACATTTTGTTACCCAGCATGATCTGCTTAGAGAGCTGGCTATCTATGAGACAAGACAAGAGGACCCTGGACATAGAGAAAGACTTATTATCAACATATCTAAAGACAATTTTCCCAAGTGGTGGAGCGAACAGAAGTATCAATGCATGAAGGCTCGCCTGTTATCTATCTCAACTGATGGAGAGTTTTCAACAAAATGGCACGACATGGAACTACCAGTAGCTGAGGTTCTAGTCTTGAATTGTCAGACCAAGAACTATGCATTACCCCAATTTGTGGAGAAAATGGTTAAGTTGAAGGTACTGGTAGTCACAAATTATGGTATTGTGCCTTCAGAATTAACAAATTTTGAAATACTGTGTACATCATCAAATTTGAAGAGAATAAGATTGGAACGCATCTCAATCCCATCCATAGCCAAGCACCCCATGCAATTGAATGGTCTCCAGAAGATTTCTTTATTCATGTGTAATATTGGTCAAGCTTTCAGCAATGGGTCAATCCAATTTTCACATGCATGGCCAAATGTAGTGGAAGTGAACATTGACTATTGCAATGATTTGGTGGCATTGCCTGCTGACACCAGTGATTTGATTCATCTAAAGAAGCTCAGCATCACCAACTGTCATAAGCTACTTGCCTTGCCTGAAGATATTGGAAAGCTGGCCAAATTAGAATTGTTGAGGCTAAGGTCTTGTACAGGCTTAGCAAAGTTGCCGGGTTCGACTGGGAACCTCAAGAAGCTAAAATCTCTTGATATATCTTACTGCTTCAGCATTAAGGAATTGCCTGAAGACTTTGGTGAATTAAGCAGCTTAAGAGAGCTTAACATGAGAGACTGCTCAAGATTGCAAGAGCTGCCTCTATCAGTTTCAAATCTGGAGCATTTAAAAGTGATAGGTGATGATGAGACCCAAAGTTTATGGCAACCCTTCTTACCTATCAAAGGCACACACATAGTGGTGGCCAAGGAGAATATCAACCTAAATTGGCTCCCCAAGTTTCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

853

Amino Acids

96.62

Weight (kDa)

5.93

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 138 4.2e-26 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 211 - 342 2.2e-17 NB-ARC domain
AAA_22 PF13401 224 - 315 9.5e-06 AAA domain
LRR_14 PF23598 712 - 815 5.2e-12 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1671, 1825
AccBSI CCGCTC 1 cut(s) 689
AciI CCGC 4 cut(s) 190, 258, 687, 1209
AclWI GGATC 3 cut(s) 189, 961, 1527
AcoI YGGCCR 5 cut(s) 3, 1191, 2069, 2214, 2514
AcsI RAATTY 7 cut(s) 725, 755, 893, 980, 1025, 1894, 1921
AcuI CTGAAG 6 cut(s) 662, 1398, 1866, 2217, 2305, 2361
AcvI CACGTG 1 cut(s) 882
AdeI CACNNNGTG 1 cut(s) 2509
AfaI GTAC 7 cut(s) 284, 293, 299, 591, 1851, 1912, 2248
AfiI CCNNNNNNNGG 7 cut(s) 196, 394, 835, 1037, 1671, 1825, 2495
AflII CTTAAG 1 cut(s) 2365
AflIII ACRYGT 1 cut(s) 106
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 2 cut(s) 302, 1615
AleI CACNNNNGTG 1 cut(s) 880
Alw21I GWGCWC 2 cut(s) 60, 246
Alw26I GTCTC 8 cut(s) 377, 467, 1274, 1324, 1592, 1997, 2379, 2453
AlwI GGATC 3 cut(s) 189, 961, 1527
AlwNI CAGNNNCTG 2 cut(s) 593, 1772
AoxI GGCC 7 cut(s) 3, 67, 626, 1191, 2069, 2214, 2514
ApeKI GCWGC 6 cut(s) 886, 1097, 1400, 1525, 2361, 2407
ApoI RAATTY 7 cut(s) 725, 755, 893, 980, 1025, 1894, 1921
Asp700I GAANNNNTTC 2 cut(s) 653, 1086
AspA2I CCTAGG 1 cut(s) 1363
AspS9I GGNCC 4 cut(s) 187, 626, 1251, 1612
AsuC2I CCSGG 4 cut(s) 28, 194, 693, 2268
AsuHPI GGTGA 5 cut(s) 409, 1471, 2158, 2363, 2462
AsuII TTCGAA 2 cut(s) 527, 984
AvaII GGWCC 3 cut(s) 187, 1251, 1612
AvrII CCTAGG 1 cut(s) 1363
BaeI ACNNNNGTAYC 4 cut(s) 1531, 1564, 1670, 1703
BalI TGGCCA 4 cut(s) 5, 2071, 2216, 2516
BarI GAAGNNNNNNTAC 6 cut(s) 281, 313, 582, 614, 1864, 1896
BbrPI CACGTG 1 cut(s) 882
BbsI GAAGAC 3 cut(s) 419, 1452, 2349
Bbv12I GWGCWC 2 cut(s) 60, 246
BbvCI CCTCAGC 1 cut(s) 1773
BbvI GCAGC 6 cut(s) 873, 1109, 1387, 1512, 2373, 2394
BccI CCATC 7 cut(s) 59, 312, 368, 617, 1529, 1721, 1966
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 2 cut(s) 304, 1617
BciVI GTATCC 1 cut(s) 1532
BcnI CCSGG 4 cut(s) 28, 194, 693, 2268
BcoDI GTCTC 8 cut(s) 377, 467, 1274, 1324, 1592, 1997, 2379, 2453
BfaI CTAG 7 cut(s) 206, 215, 227, 1364, 1439, 1493, 1781
BfmI CTRYAG 4 cut(s) 491, 1218, 1526, 1543
BfrI CTTAAG 1 cut(s) 2365
BfuI GTATCC 1 cut(s) 1532
BglII AGATCT 1 cut(s) 1019
BisI GCNGC 7 cut(s) 259, 887, 1098, 1401, 1526, 2362, 2408
BlnI CCTAGG 1 cut(s) 1363
BlpI GCTNAGC 2 cut(s) 2159, 2254
BlsI GCNGC 7 cut(s) 260, 888, 1099, 1402, 1527, 2363, 2409
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 6 cut(s) 28, 194, 304, 693, 1617, 2268
Bme18I GGWCC 3 cut(s) 187, 1251, 1612
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 4 cut(s) 187, 626, 1251, 1612
BmiI GGNNCC 3 cut(s) 1614, 2282, 2543
BmrFI CCNGG 6 cut(s) 28, 194, 304, 693, 1617, 2268
BmrI ACTGGG 1 cut(s) 2286
BmsI GCATC 4 cut(s) 339, 1109, 1956, 2172
BmuI ACTGGG 1 cut(s) 2286
BpiI GAAGAC 3 cut(s) 419, 1452, 2349
BpmI CTGGAG 4 cut(s) 164, 286, 1979, 2450
Bpu10I CCTNAGC 2 cut(s) 1773, 2237
Bpu1102I GCTNAGC 2 cut(s) 2159, 2254
Bpu14I TTCGAA 2 cut(s) 527, 984
BpuEI CTTGAG 3 cut(s) 56, 2270, 2377
BpuMI CCSGG 4 cut(s) 28, 194, 693, 2268
BsaAI YACGTR 1 cut(s) 882
BsaI GGTCTC 4 cut(s) 377, 467, 1997, 2453
BsaJI CCNNGG 5 cut(s) 27, 1104, 1363, 1615, 2517
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 7 cut(s) 196, 394, 835, 1037, 1671, 1825, 2495
Bse1I ACTGG 7 cut(s) 11, 438, 1308, 1766, 1857, 2133, 2281
Bse3DI GCAATG 4 cut(s) 1083, 2047, 2110, 2117
BseBI CCWGG 2 cut(s) 304, 1617
BseDI CCNNGG 5 cut(s) 27, 1104, 1363, 1615, 2517
BseGI GGATG 8 cut(s) 22, 323, 628, 704, 943, 1100, 1540, 1958
BseLI CCNNNNNNNGG 7 cut(s) 196, 394, 835, 1037, 1671, 1825, 2495
BseMI GCAATG 4 cut(s) 1083, 2047, 2110, 2117
BseMII CTCAG 4 cut(s) 890, 1413, 1764, 2173
BseNI ACTGG 7 cut(s) 11, 438, 1308, 1766, 1857, 2133, 2281
BseXI GCAGC 6 cut(s) 873, 1109, 1387, 1512, 2373, 2394
BseYI CCCAGC 2 cut(s) 539, 1563
BsgI GTGCAG 1 cut(s) 606
BshFI GGCC 7 cut(s) 5, 69, 628, 1193, 2071, 2216, 2516
BsiHKAI GWGCWC 2 cut(s) 60, 246
BsiSI CCGG 5 cut(s) 27, 194, 693, 1194, 2267
BslI CCNNNNNNNGG 7 cut(s) 196, 394, 835, 1037, 1671, 1825, 2495
BsmAI GTCTC 8 cut(s) 377, 467, 1274, 1324, 1592, 1997, 2379, 2453
BsnI GGCC 7 cut(s) 5, 69, 628, 1193, 2071, 2216, 2516
Bso31I GGTCTC 4 cut(s) 377, 467, 1997, 2453
Bsp119I TTCGAA 2 cut(s) 527, 984
Bsp1286I GDGCHC 2 cut(s) 60, 246
Bsp1407I TGTACA 3 cut(s) 282, 1910, 2246
Bsp143I GATC 7 cut(s) 181, 953, 1019, 1381, 1476, 1519, 1570
Bsp1720I GCTNAGC 2 cut(s) 2159, 2254
BspACI CCGC 4 cut(s) 190, 258, 687, 1209
BspANI GGCC 7 cut(s) 5, 69, 628, 1193, 2071, 2216, 2516
BspCNI CTCAG 4 cut(s) 891, 1414, 1765, 2172
BspLI GGNNCC 3 cut(s) 1614, 2282, 2543
BspMAI CTGCAG 1 cut(s) 1530
BspPI GGATC 3 cut(s) 189, 961, 1527
BspT104I TTCGAA 2 cut(s) 527, 984
BspTI CTTAAG 1 cut(s) 2365
BspTNI GGTCTC 4 cut(s) 377, 467, 1997, 2453
BsrBI CCGCTC 1 cut(s) 689
BsrDI GCAATG 4 cut(s) 1083, 2047, 2110, 2117
BsrGI TGTACA 3 cut(s) 282, 1910, 2246
BsrI ACTGG 7 cut(s) 11, 438, 1308, 1766, 1857, 2133, 2281
BssECI CCNNGG 5 cut(s) 27, 1104, 1363, 1615, 2517
BssMI GATC 7 cut(s) 181, 953, 1019, 1381, 1476, 1519, 1570
BssT1I CCWWGG 3 cut(s) 1104, 1363, 2517
Bst2UI CCWGG 2 cut(s) 304, 1617
Bst6I CTCTTC 2 cut(s) 395, 1922
BstAFI CTTAAG 1 cut(s) 2365
BstAPI GCANNNNNTGC 4 cut(s) 1573, 1978, 2123, 2407
BstAUI TGTACA 3 cut(s) 282, 1910, 2246
BstBAI YACGTR 1 cut(s) 882
BstBI TTCGAA 2 cut(s) 527, 984
BstC8I GCNNGC 5 cut(s) 877, 1587, 1705, 2124, 2214
BstDEI CTNAG 8 cut(s) 899, 1357, 1422, 1577, 1773, 2159, 2237, 2254
BstENI CCTNNNNNAGG 1 cut(s) 2493
BstF5I GGATG 8 cut(s) 22, 323, 628, 704, 943, 1100, 1540, 1958
BstKTI GATC 7 cut(s) 184, 956, 1022, 1384, 1479, 1522, 1573
BstMAI GTCTC 8 cut(s) 377, 467, 1274, 1324, 1592, 1997, 2379, 2453
BstMBI GATC 7 cut(s) 181, 953, 1019, 1381, 1476, 1519, 1570
BstMWI GCNNNNNNNGC 7 cut(s) 584, 840, 1573, 1582, 1978, 2123, 2407
BstNI CCWGG 2 cut(s) 304, 1617
BstNSI RCATGY 1 cut(s) 2066
BstSCI CCNGG 6 cut(s) 26, 192, 302, 691, 1615, 2266
BstSFI CTRYAG 4 cut(s) 491, 1218, 1526, 1543
BstV1I GCAGC 6 cut(s) 873, 1109, 1387, 1512, 2373, 2394
BstV2I GAAGAC 3 cut(s) 419, 1452, 2349
BstX2I RGATCY 2 cut(s) 181, 1019
BstXI CCANNNNNNTGG 1 cut(s) 13
BstYI RGATCY 2 cut(s) 181, 1019
BsuI GTATCC 1 cut(s) 1532
BsuRI GGCC 7 cut(s) 5, 69, 628, 1193, 2071, 2216, 2516
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 8 cut(s) 22, 323, 628, 704, 943, 1100, 1540, 1958
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 6 cut(s) 18, 480, 780, 1194, 1552, 2140
Cac8I GCNNGC 5 cut(s) 877, 1587, 1705, 2124, 2214
CaiI CAGNNNCTG 2 cut(s) 593, 1772
Cfr13I GGNCC 4 cut(s) 187, 626, 1251, 1612
CseI GACGC 1 cut(s) 109
Csp6I GTAC 7 cut(s) 283, 292, 298, 590, 1850, 1911, 2247
CspCI CAANNNNNGTGG 2 cut(s) 2061, 2096
CviQI GTAC 7 cut(s) 283, 292, 298, 590, 1850, 1911, 2247
DdeI CTNAG 8 cut(s) 899, 1357, 1422, 1577, 1773, 2159, 2237, 2254
DpnI GATC 7 cut(s) 183, 955, 1021, 1383, 1478, 1521, 1572
DpnII GATC 7 cut(s) 181, 953, 1019, 1381, 1476, 1519, 1570
DraI TTTAAA 2 cut(s) 859, 2439
DraIII CACNNNGTG 1 cut(s) 2509
EaeI YGGCCR 5 cut(s) 3, 1191, 2069, 2214, 2514
Eam1104I CTCTTC 2 cut(s) 395, 1922
EarI CTCTTC 2 cut(s) 395, 1922
Eco130I CCWWGG 3 cut(s) 1104, 1363, 2517
Eco31I GGTCTC 4 cut(s) 377, 467, 1997, 2453
Eco32I GATATC 1 cut(s) 1414
Eco47I GGWCC 3 cut(s) 187, 1251, 1612
Eco57I CTGAAG 6 cut(s) 662, 1398, 1866, 2217, 2305, 2361
Eco72I CACGTG 1 cut(s) 882
EcoNI CCTNNNNNAGG 1 cut(s) 2493
EcoO109I RGGNCCY 1 cut(s) 1612
EcoRI GAATTC 1 cut(s) 980
EcoRII CCWGG 2 cut(s) 302, 1615
EcoRV GATATC 1 cut(s) 1414
EcoT14I CCWWGG 3 cut(s) 1104, 1363, 2517
EcoT22I ATGCAT 3 cut(s) 1697, 1813, 2068
ErhI CCWWGG 3 cut(s) 1104, 1363, 2517
Fnu4HI GCNGC 7 cut(s) 259, 887, 1098, 1401, 1526, 2362, 2408
FokI GGATG 8 cut(s) 29, 330, 635, 711, 950, 1087, 1547, 1945
Fsp4HI GCNGC 7 cut(s) 259, 887, 1098, 1401, 1526, 2362, 2408
FspBI CTAG 7 cut(s) 206, 215, 227, 1364, 1439, 1493, 1781
GluI GCNGC 7 cut(s) 259, 887, 1098, 1401, 1526, 2362, 2408
GsaI CCCAGC 2 cut(s) 543, 1567
GsuI CTGGAG 4 cut(s) 164, 286, 1979, 2450
HaeIII GGCC 7 cut(s) 5, 69, 628, 1193, 2071, 2216, 2516
HapII CCGG 5 cut(s) 27, 194, 693, 1194, 2267
HgaI GACGC 1 cut(s) 109
HindIII AAGCTT 2 cut(s) 576, 2031
HpaII CCGG 5 cut(s) 27, 194, 693, 1194, 2267
HphI GGTGA 5 cut(s) 409, 1471, 2158, 2363, 2462
Hpy166II GTNNAC 5 cut(s) 283, 1436, 1550, 1911, 2089
Hpy188I TCNGA 7 cut(s) 900, 958, 1024, 1032, 1255, 1797, 1885
Hpy8I GTNNAC 5 cut(s) 283, 1436, 1550, 1911, 2089
HpyAV CCTTC 8 cut(s) 226, 373, 902, 1510, 1693, 1840, 1890, 2491
HpyCH4IV ACGT 2 cut(s) 108, 881
HpyF10VI GCNNNNNNNGC 7 cut(s) 584, 840, 1573, 1582, 1978, 2123, 2407
HpyF3I CTNAG 8 cut(s) 899, 1357, 1422, 1577, 1773, 2159, 2237, 2254
HpySE526I ACGT 2 cut(s) 108, 881
Kzo9I GATC 7 cut(s) 181, 953, 1019, 1381, 1476, 1519, 1570
LmnI GCTCC 7 cut(s) 76, 145, 463, 694, 1674, 2431, 2547
Lsp1109I GCAGC 6 cut(s) 873, 1109, 1387, 1512, 2373, 2394
LweI GCATC 4 cut(s) 339, 1109, 1956, 2172
MaeI CTAG 7 cut(s) 206, 215, 227, 1364, 1439, 1493, 1781
MaeII ACGT 2 cut(s) 108, 881
MaeIII GTNAC 5 cut(s) 778, 1009, 1507, 1558, 1858
MalI GATC 7 cut(s) 183, 955, 1021, 1383, 1478, 1521, 1572
MbiI CCGCTC 1 cut(s) 689
MboI GATC 7 cut(s) 181, 953, 1019, 1381, 1476, 1519, 1570
MfeI CAATTG 2 cut(s) 1286, 1982
MflI RGATCY 2 cut(s) 181, 1019
MhlI GDGCHC 2 cut(s) 60, 246
MlsI TGGCCA 4 cut(s) 5, 2071, 2216, 2516
MluNI TGGCCA 4 cut(s) 5, 2071, 2216, 2516
MlyI GAGTC 5 cut(s) 220, 953, 1233, 1276, 1433
MmeI TCCRAC 3 cut(s) 324, 595, 626
Mox20I TGGCCA 4 cut(s) 5, 2071, 2216, 2516
Mph1103I ATGCAT 3 cut(s) 1697, 1813, 2068
MroXI GAANNNNTTC 2 cut(s) 653, 1086
MscI TGGCCA 4 cut(s) 5, 2071, 2216, 2516
MslI CAYNNNNRTG 3 cut(s) 880, 2065, 2510
Msp20I TGGCCA 4 cut(s) 5, 2071, 2216, 2516
MspA1I CMGCKG 1 cut(s) 1525
MspCI CTTAAG 1 cut(s) 2365
MspI CCGG 5 cut(s) 27, 194, 693, 1194, 2267
MspR9I CCNGG 6 cut(s) 28, 194, 304, 693, 1617, 2268
MunI CAATTG 2 cut(s) 1286, 1982
MvaI CCWGG 2 cut(s) 304, 1617
MwoI GCNNNNNNNGC 7 cut(s) 584, 840, 1573, 1582, 1978, 2123, 2407
NciI CCSGG 4 cut(s) 28, 194, 693, 2268
NdeII GATC 7 cut(s) 181, 953, 1019, 1381, 1476, 1519, 1570
NlaIV GGNNCC 3 cut(s) 1614, 2282, 2543
NmeAIII GCCGAG 1 cut(s) 533
NmuCI GTSAC 4 cut(s) 778, 1009, 1507, 1858
NsiI ATGCAT 3 cut(s) 1697, 1813, 2068
NspI RCATGY 1 cut(s) 2066
NspV TTCGAA 2 cut(s) 527, 984
OliI CACNNNNGTG 1 cut(s) 880
PdmI GAANNNNTTC 2 cut(s) 653, 1086
PfeI GAWTC 5 cut(s) 137, 431, 1267, 1389, 2143
PflFI GACNNNGTC 1 cut(s) 1423
PflMI CCANNNNNTGG 2 cut(s) 1671, 1825
PfoI TCCNGGA 2 cut(s) 302, 691
PkrI GCNGC 7 cut(s) 260, 888, 1099, 1402, 1527, 2363, 2409
PleI GAGTC 5 cut(s) 219, 953, 1233, 1276, 1432
PmaCI CACGTG 1 cut(s) 882
PmlI CACGTG 1 cut(s) 882
PpsI GAGTC 5 cut(s) 219, 953, 1233, 1276, 1432
Ppu21I YACGTR 1 cut(s) 882
PpuMI RGGWCCY 1 cut(s) 1612
Psp5II RGGWCCY 1 cut(s) 1612
Psp6I CCWGG 2 cut(s) 302, 1615
PspCI CACGTG 1 cut(s) 882
PspFI CCCAGC 2 cut(s) 539, 1563
PspGI CCWGG 2 cut(s) 302, 1615
PspN4I GGNNCC 3 cut(s) 1614, 2282, 2543
PspPI GGNCC 4 cut(s) 187, 626, 1251, 1612
PspPPI RGGWCCY 1 cut(s) 1612
PsrI GAACNNNNNNTAC 4 cut(s) 83, 115, 1761, 1793
PstI CTGCAG 1 cut(s) 1530
PstNI CAGNNNCTG 2 cut(s) 593, 1772
PsuI RGATCY 2 cut(s) 181, 1019
PsyI GACNNNGTC 1 cut(s) 1423
PvuII CAGCTG 1 cut(s) 1525
RsaI GTAC 7 cut(s) 284, 293, 299, 591, 1851, 1912, 2248
RsaNI GTAC 7 cut(s) 283, 292, 298, 590, 1850, 1911, 2247
RseI CAYNNNNRTG 3 cut(s) 880, 2065, 2510
SatI GCNGC 7 cut(s) 259, 887, 1098, 1401, 1526, 2362, 2408
Sau3AI GATC 7 cut(s) 181, 953, 1019, 1381, 1476, 1519, 1570
Sau96I GGNCC 4 cut(s) 187, 626, 1251, 1612
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 5 cut(s) 220, 953, 1233, 1276, 1433
ScrFI CCNGG 6 cut(s) 28, 194, 304, 693, 1617, 2268
SduI GDGCHC 2 cut(s) 60, 246
SfaNI GCATC 4 cut(s) 339, 1109, 1956, 2172
SfcI CTRYAG 4 cut(s) 491, 1218, 1526, 1543
SfuI TTCGAA 2 cut(s) 527, 984
SinI GGWCC 3 cut(s) 187, 1251, 1612
SmiMI CAYNNNNRTG 3 cut(s) 880, 2065, 2510
SmlI CTYRAG 4 cut(s) 71, 2285, 2365, 2392
SmoI CTYRAG 4 cut(s) 71, 2285, 2365, 2392
SsiI CCGC 4 cut(s) 190, 258, 687, 1209
SspI AATATT 2 cut(s) 769, 2023
SspMI CTAG 7 cut(s) 206, 215, 227, 1364, 1439, 1493, 1781
StyD4I CCNGG 6 cut(s) 26, 192, 302, 691, 1615, 2266
StyI CCWWGG 3 cut(s) 1104, 1363, 2517
TaiI ACGT 2 cut(s) 111, 884
TaqI TCGA 9 cut(s) 135, 429, 434, 527, 984, 1265, 1278, 1322, 2273
TatI WGTACW 5 cut(s) 282, 291, 297, 1910, 2246
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 5 cut(s) 137, 431, 1267, 1389, 2143
TscAI CASTG 6 cut(s) 18, 487, 787, 1194, 1552, 2140
TseFI GTSAC 4 cut(s) 778, 1009, 1507, 1858
TseI GCWGC 6 cut(s) 886, 1097, 1400, 1525, 2361, 2407
Tsp45I GTSAC 4 cut(s) 778, 1009, 1507, 1858
TspDTI ATGAA 7 cut(s) 350, 662, 1359, 1458, 1712, 2002, 2135
TspRI CASTG 6 cut(s) 18, 487, 787, 1194, 1552, 2140
Tth111I GACNNNGTC 1 cut(s) 1423
Van91I CCANNNNNTGG 2 cut(s) 1671, 1825
Vha464I CTTAAG 1 cut(s) 2365
VpaK11BI GGWCC 3 cut(s) 187, 1251, 1612
XagI CCTNNNNNAGG 1 cut(s) 2493
XapI RAATTY 7 cut(s) 725, 755, 893, 980, 1025, 1894, 1921
XbaI TCTAGA 1 cut(s) 214
XceI RCATGY 1 cut(s) 2066
XmaJI CCTAGG 1 cut(s) 1363
XmnI GAANNNNTTC 2 cut(s) 653, 1086
XspI CTAG 7 cut(s) 206, 215, 227, 1364, 1439, 1493, 1781
ZrmI AGTACT 1 cut(s) 299
Zsp2I ATGCAT 3 cut(s) 1697, 1813, 2068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.