Rh2DG225500

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000523
Physical Location & Seq
Forward (+)
22058538 .. 22064512
5975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG225500.1

Sequence Viewer

Length: 1560 bp
ATGGAGAAGATGAGGAAATGGGGTTGGCTTCTGGGGTCATCAGCAGCAATTCTTCTTCTGGTTTTGTTCTTGGCTGGTCATGGAACTTACGACGAGACGACGAGCCGAGTAAGACGATCACTGAGGAGCATAATTCCTCGGCGACAACAGGGGGTCAATTTACTAACAAGGGACCCCAAGAGGGTTGTTTTGGATAATGGCATTGTCAAAGTCATTTTCTCTAATCCGGGCGGATACGTTATTGGTATACAGTACAGAGGCCTTGACATACTTGAAATGGGGAATGAAGAAAATAATAGAGGGTACTGGGATGTGGTCTGGAAAAAGAATATAACTGACAAACTAGAAGCAACAAAATTTAGGGTTATTACCACGAGGCCAGACCAAGCAGAAATTTCCTTCCTAAGAACATATGATGTTTCCCTTGGTAACGGCACGAACACAATTCCCTTGACCGTAGACAAAAGGTACATACTGCAGCGGGGTGGTTCTGGTTTCTATGCATATGGAATATTCGAGCGCCTTGATGGGTGGCCGGCGGTACAGGATATGGATCAAATTAGAATGGTTTACAAGCTTGAACAAGCCAAATTTCATTATATGGCGTTATCAGACACCAGGCAAAGAAGCATGCCAACAGCAGCAGACCGTGGTCGTGGTCAGAAACTTGCGTATAAAGAAGCTTGTCTTTTGACTAATCCAAGCAATCCTGACTTCAGCGGAGAGGTAGATGACAAGTACCAGTACTCAATCGAGGACAAAGATAACAAAGTTCATGGTTGGATTAGTACTGATGAATCTGTGGGATTCTGGATGATCACACCCAGCGACGAATTTCGTACAGGAGGCCCCTTTAAGCAGGACCTCACCTCCCATGTTGGTCCAACCACCCTCTCCATGTTTGTTAGTACTCATTATGCTGGGAAGGAACTTCAGATCAAATTTGAAGAAGGTGAGGCATGGAAAAAGGTTTTTGGGCCTGTCTTTGTGTATCTTAATTCGGCTCCAAGCTCAAAGAATTCCATCCGTATACTTTGGAATGATGCTAAAAAGCAGATGCTTAAAGAAGTCAAGAACTGGCCGTATAATTTCACTCAATCCGAGGACTTTCCTTCTTCCCATCAACGGGGATCAGTTTTGGGCCAATTACTAATACATGATCGATACATCAATGAGAGTCTTTTCCCAGCAAGTTCTGCTTATGTGGGATTGGCTGCACCCGGAGAAGTGGGATCATGGCAAAGGGAAAACAAGGGTTATCAATTTTGGAATCAAGCTGACGAAGAAGGTAGATTCATTATCAAAAATGTTCGACCCGGAAACTATAGTTTTTATGCATGGGTTCCTGGCATTGTTGGGGATTACAAATATGAGGTTGATATTACAATTACTCCAGGGTCTAATATCAAATTGGACAATCTTACTTACGAACCTCCAAGAAATGGTCCTACTTTGTGGGAAATTGGCATCCCTGATCGCACTGCTGCCGAATTCTACGTACCAGATCCTTATCCAACTCTTTTGAACAATTTATACACCCAGAATCACACAGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

519

Amino Acids

59.29

Weight (kDa)

7.7

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 52 - 243 1.2e-72 Rhamnogalacturonate lyase family
fn3_3 PF14686 398 - 471 3.4e-25 Polysaccharide lyase family 4, domain II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1442
AccI GTMKAC 3 cut(s) 247, 459, 1030
AciI CCGC 4 cut(s) 231, 481, 539, 720
AclWI GGATC 4 cut(s) 561, 1138, 1240, 1499
AcoI YGGCCR 2 cut(s) 533, 1079
AcsI RAATTY 7 cut(s) 356, 393, 590, 833, 941, 1018, 1490
AcuI CTGAAG 2 cut(s) 700, 917
AfiI CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
AgsI TTSAA 4 cut(s) 275, 581, 947, 1525
AjnI CCWGG 3 cut(s) 617, 1345, 1393
AluBI AGCT 4 cut(s) 577, 683, 1011, 1277
AluI AGCT 4 cut(s) 577, 683, 1011, 1277
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 4 cut(s) 561, 1138, 1240, 1499
AoxI GGCC 7 cut(s) 259, 377, 533, 847, 977, 1079, 1141
ApeKI GCWGC 5 cut(s) 44, 478, 641, 1214, 1484
ApoI RAATTY 7 cut(s) 356, 393, 590, 833, 941, 1018, 1490
AspLEI GCGC 1 cut(s) 522
AspS9I GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
AsuC2I CCSGG 3 cut(s) 228, 1221, 1317
AsuHPI GGTGA 2 cut(s) 859, 965
AvaII GGWCC 4 cut(s) 172, 862, 881, 1445
BauI CACGAG 1 cut(s) 373
BbvI GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
BccI CCATC 3 cut(s) 521, 1031, 1128
BceAI ACGGC 2 cut(s) 448, 1066
BciT130I CCWGG 3 cut(s) 619, 1347, 1395
BciVI GTATCC 1 cut(s) 227
BclI TGATCA 1 cut(s) 816
BcnI CCSGG 3 cut(s) 228, 1221, 1317
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 1 cut(s) 344
BfmI CTRYAG 2 cut(s) 476, 1324
BfoI RGCGCY 1 cut(s) 523
BfuI GTATCC 1 cut(s) 227
BisI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
BlsI GCNGC 5 cut(s) 46, 480, 643, 1216, 1486
BmcAI AGTACT 3 cut(s) 746, 790, 910
Bme1390I CCNGG 6 cut(s) 228, 619, 1221, 1317, 1347, 1395
Bme18I GGWCC 4 cut(s) 172, 862, 881, 1445
BmgT120I GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
BmiI GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
BmrFI CCNGG 6 cut(s) 228, 619, 1221, 1317, 1347, 1395
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 3 cut(s) 1033, 1047, 1476
BmuI ACTGGG 1 cut(s) 316
BoxI GACNNNNGTC 1 cut(s) 651
BpmI CTGGAG 1 cut(s) 1377
BpuMI CCSGG 3 cut(s) 228, 1221, 1317
Bsa29I ATCGAT 1 cut(s) 1162
BsaAI YACGTR 1 cut(s) 1498
BsaBI GATNNNNATC 2 cut(s) 552, 1509
BsaJI CCNNGG 5 cut(s) 137, 424, 649, 1101, 1394
BsaXI ACNNNNNCTCC 4 cut(s) 854, 884, 1375, 1405
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
Bse118I RCCGGY 1 cut(s) 535
Bse1I ACTGG 3 cut(s) 311, 742, 1082
Bse8I GATNNNNATC 2 cut(s) 552, 1509
BseBI CCWGG 3 cut(s) 619, 1347, 1395
BseCI ATCGAT 1 cut(s) 1162
BseDI CCNNGG 5 cut(s) 137, 424, 649, 1101, 1394
BseGI GGATG 4 cut(s) 316, 819, 1023, 1467
BseJI GATNNNNATC 2 cut(s) 552, 1509
BseLI CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 3 cut(s) 311, 742, 1082
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
BseYI CCCAGC 3 cut(s) 824, 920, 1186
BsgI GTGCAG 1 cut(s) 1200
BshFI GGCC 7 cut(s) 261, 379, 535, 849, 979, 1081, 1143
BshVI ATCGAT 1 cut(s) 1162
BsiSI CCGG 4 cut(s) 227, 536, 1221, 1317
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
BsmAI GTCTC 1 cut(s) 89
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 185
BsnI GGCC 7 cut(s) 261, 379, 535, 849, 979, 1081, 1143
Bsp143I GATC 9 cut(s) 116, 553, 816, 936, 1130, 1159, 1232, 1474, 1504
BspACI CCGC 4 cut(s) 231, 481, 539, 720
BspANI GGCC 7 cut(s) 261, 379, 535, 849, 979, 1081, 1143
BspCNI CTCAG 1 cut(s) 114
BspDI ATCGAT 1 cut(s) 1162
BspLI GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
BspMAI CTGCAG 1 cut(s) 480
BspPI GGATC 4 cut(s) 561, 1138, 1240, 1499
BsrFI RCCGGY 1 cut(s) 535
BsrI ACTGG 3 cut(s) 311, 742, 1082
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 5 cut(s) 137, 424, 649, 1101, 1394
BssMI GATC 9 cut(s) 116, 553, 816, 936, 1130, 1159, 1232, 1474, 1504
BssNAI GTATAC 2 cut(s) 248, 1031
BssSI CACGAG 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 424
Bst1107I GTATAC 2 cut(s) 248, 1031
Bst2BI CACGAG 1 cut(s) 373
Bst2UI CCWGG 3 cut(s) 619, 1347, 1395
Bst4CI ACNGT 3 cut(s) 252, 457, 650
BstAPI GCANNNNNTGC 1 cut(s) 1196
BstBAI YACGTR 1 cut(s) 1498
BstC8I GCNNGC 2 cut(s) 537, 632
BstDEI CTNAG 2 cut(s) 122, 404
BstDSI CCRYGG 1 cut(s) 649
BstF5I GGATG 4 cut(s) 316, 819, 1023, 1467
BstH2I RGCGCY 1 cut(s) 523
BstHHI GCGC 1 cut(s) 522
BstKTI GATC 9 cut(s) 119, 556, 819, 939, 1133, 1162, 1235, 1477, 1507
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 9 cut(s) 116, 553, 816, 936, 1130, 1159, 1232, 1474, 1504
BstMWI GCNNNNNNNGC 1 cut(s) 1196
BstNI CCWGG 3 cut(s) 619, 1347, 1395
BstNSI RCATGY 1 cut(s) 634
BstPAI GACNNNNGTC 1 cut(s) 651
BstSCI CCNGG 6 cut(s) 226, 617, 1219, 1315, 1345, 1393
BstSFI CTRYAG 2 cut(s) 476, 1324
BstSNI TACGTA 1 cut(s) 1498
BstV1I GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
BstX2I RGATCY 1 cut(s) 1504
BstYI RGATCY 1 cut(s) 1504
BstZ17I GTATAC 2 cut(s) 248, 1031
Bsu15I ATCGAT 1 cut(s) 1162
BsuI GTATCC 1 cut(s) 227
BsuRI GGCC 7 cut(s) 261, 379, 535, 849, 979, 1081, 1143
BsuTUI ATCGAT 1 cut(s) 1162
BtgI CCRYGG 1 cut(s) 649
BtsCI GGATG 4 cut(s) 316, 819, 1023, 1467
BtsI GCAGTG 1 cut(s) 1479
BtsIMutI CAGTG 2 cut(s) 119, 1479
Cac8I GCNNGC 2 cut(s) 537, 632
CfoI GCGC 1 cut(s) 522
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
ClaI ATCGAT 1 cut(s) 1162
CviAII CATG 9 cut(s) 80, 631, 776, 875, 898, 960, 1157, 1236, 1338
DdeI CTNAG 2 cut(s) 122, 404
DpnI GATC 9 cut(s) 118, 555, 818, 938, 1132, 1161, 1234, 1476, 1506
DpnII GATC 9 cut(s) 116, 553, 816, 936, 1130, 1159, 1232, 1474, 1504
EaeI YGGCCR 2 cut(s) 533, 1079
EciI GGCGGA 1 cut(s) 246
Eco105I TACGTA 1 cut(s) 1498
Eco130I CCWWGG 1 cut(s) 424
Eco147I AGGCCT 1 cut(s) 261
Eco47I GGWCC 4 cut(s) 172, 862, 881, 1445
Eco57I CTGAAG 2 cut(s) 700, 917
EcoO109I RGGNCCY 3 cut(s) 172, 848, 862
EcoRI GAATTC 2 cut(s) 1018, 1490
EcoRII CCWGG 3 cut(s) 617, 1345, 1393
EcoT14I CCWWGG 1 cut(s) 424
EcoT22I ATGCAT 2 cut(s) 505, 1339
ErhI CCWWGG 1 cut(s) 424
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 9 cut(s) 83, 634, 779, 878, 901, 963, 1160, 1239, 1341
FalI AAGNNNNNCTT 4 cut(s) 672, 704, 1183, 1215
FaqI GGGAC 1 cut(s) 185
FatI CATG 9 cut(s) 79, 630, 775, 874, 897, 959, 1156, 1235, 1337
FauI CCCGC 1 cut(s) 474
FauNDI CATATG 2 cut(s) 412, 505
FbaI TGATCA 1 cut(s) 816
FblI GTMKAC 3 cut(s) 247, 459, 1030
Fnu4HI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
FokI GGATG 4 cut(s) 323, 826, 1010, 1454
Fsp4HI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
FspBI CTAG 1 cut(s) 344
GlaI GCGC 1 cut(s) 521
GluI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
GsaI CCCAGC 3 cut(s) 828, 924, 1190
GsuI CTGGAG 1 cut(s) 1377
HaeII RGCGCY 1 cut(s) 523
HaeIII GGCC 7 cut(s) 261, 379, 535, 849, 979, 1081, 1143
HapII CCGG 4 cut(s) 227, 536, 1221, 1317
HhaI GCGC 1 cut(s) 522
Hin1II CATG 9 cut(s) 83, 634, 779, 878, 901, 963, 1160, 1239, 1341
Hin6I GCGC 1 cut(s) 520
HinP1I GCGC 1 cut(s) 520
HindIII AAGCTT 2 cut(s) 575, 681
HinfI GANTC 6 cut(s) 797, 807, 1177, 1270, 1293, 1543
HpaII CCGG 4 cut(s) 227, 536, 1221, 1317
HphI GGTGA 2 cut(s) 859, 965
Hpy166II GTNNAC 4 cut(s) 248, 460, 571, 1031
Hpy188I TCNGA 4 cut(s) 613, 663, 936, 1102
Hpy188III TCNNGA 4 cut(s) 319, 710, 811, 1072
Hpy8I GTNNAC 4 cut(s) 248, 460, 571, 1031
Hpy99I CGWCG 3 cut(s) 95, 103, 833
HpyAV CCTTC 5 cut(s) 409, 919, 944, 1122, 1280
HpyCH4III ACNGT 3 cut(s) 252, 457, 650
HpyCH4IV ACGT 2 cut(s) 237, 1497
HpyCH4V TGCA 4 cut(s) 478, 503, 1217, 1337
HpyF10VI GCNNNNNNNGC 1 cut(s) 1196
HpyF3I CTNAG 2 cut(s) 122, 404
HpySE526I ACGT 2 cut(s) 237, 1497
Hsp92II CATG 9 cut(s) 83, 634, 779, 878, 901, 963, 1160, 1239, 1341
HspAI GCGC 1 cut(s) 520
KflI GGGWCCC 1 cut(s) 172
KroI GCCGGC 1 cut(s) 535
KroNI GCCGGC 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 816
Kzo9I GATC 9 cut(s) 116, 553, 816, 936, 1130, 1159, 1232, 1474, 1504
LmnI GCTCC 2 cut(s) 126, 1009
Lsp1109I GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
LweI GCATC 3 cut(s) 1033, 1047, 1476
MaeI CTAG 1 cut(s) 344
MaeII ACGT 2 cut(s) 237, 1497
MaeIII GTNAC 1 cut(s) 428
MalI GATC 9 cut(s) 118, 555, 818, 938, 1132, 1161, 1234, 1476, 1506
MboI GATC 9 cut(s) 116, 553, 816, 936, 1130, 1159, 1232, 1474, 1504
MboII GAAGA 7 cut(s) 19, 44, 47, 299, 959, 1107, 1295
MflI RGATCY 1 cut(s) 1504
MlyI GAGTC 1 cut(s) 1186
MmeI TCCRAC 3 cut(s) 761, 908, 1538
Mph1103I ATGCAT 2 cut(s) 505, 1339
MroNI GCCGGC 1 cut(s) 535
MseI TTAA 3 cut(s) 855, 996, 1062
MspA1I CMGCKG 2 cut(s) 481, 720
MspI CCGG 4 cut(s) 227, 536, 1221, 1317
MspR9I CCNGG 6 cut(s) 228, 619, 1221, 1317, 1347, 1395
MvaI CCWGG 3 cut(s) 619, 1347, 1395
MwoI GCNNNNNNNGC 1 cut(s) 1196
NaeI GCCGGC 1 cut(s) 537
NciI CCSGG 3 cut(s) 228, 1221, 1317
NdeI CATATG 2 cut(s) 412, 505
NdeII GATC 9 cut(s) 116, 553, 816, 936, 1130, 1159, 1232, 1474, 1504
NgoMIV GCCGGC 1 cut(s) 535
NlaIII CATG 9 cut(s) 83, 634, 779, 878, 901, 963, 1160, 1239, 1341
NlaIV GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
NmeAIII GCCGAG 2 cut(s) 118, 131
NsiI ATGCAT 2 cut(s) 505, 1339
NspI RCATGY 1 cut(s) 634
PaeI GCATGC 1 cut(s) 634
PceI AGGCCT 1 cut(s) 261
PdiI GCCGGC 1 cut(s) 537
PfeI GAWTC 5 cut(s) 797, 807, 1270, 1293, 1543
PflMI CCANNNNNTGG 1 cut(s) 1442
PkrI GCNGC 5 cut(s) 46, 480, 643, 1216, 1486
PleI GAGTC 1 cut(s) 1185
PpsI GAGTC 1 cut(s) 1185
Ppu21I YACGTR 1 cut(s) 1498
PpuMI RGGWCCY 2 cut(s) 172, 862
PshAI GACNNNNGTC 1 cut(s) 651
Psp5II RGGWCCY 2 cut(s) 172, 862
Psp6I CCWGG 3 cut(s) 617, 1345, 1393
PspFI CCCAGC 3 cut(s) 824, 920, 1186
PspGI CCWGG 3 cut(s) 617, 1345, 1393
PspN4I GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
PspPI GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
PspPPI RGGWCCY 2 cut(s) 172, 862
PsrI GAACNNNNNNTAC 2 cut(s) 1517, 1549
PstI CTGCAG 1 cut(s) 480
PsuI RGATCY 1 cut(s) 1504
SaqAI TTAA 3 cut(s) 855, 996, 1062
SatI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
Sau3AI GATC 9 cut(s) 116, 553, 816, 936, 1130, 1159, 1232, 1474, 1504
Sau96I GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
ScaI AGTACT 3 cut(s) 746, 790, 910
SchI GAGTC 1 cut(s) 1186
ScrFI CCNGG 6 cut(s) 228, 619, 1221, 1317, 1347, 1395
SfaNI GCATC 3 cut(s) 1033, 1047, 1476
SfcI CTRYAG 2 cut(s) 476, 1324
SinI GGWCC 4 cut(s) 172, 862, 881, 1445
SnaBI TACGTA 1 cut(s) 1498
SphI GCATGC 1 cut(s) 634
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 4 cut(s) 231, 481, 539, 720
SspI AATATT 1 cut(s) 513
SspMI CTAG 1 cut(s) 344
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 6 cut(s) 226, 617, 1219, 1315, 1345, 1393
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 3 cut(s) 252, 457, 650
TaiI ACGT 2 cut(s) 240, 1500
TaqI TCGA 4 cut(s) 516, 753, 1162, 1312
TatI WGTACW 4 cut(s) 252, 744, 788, 908
TfiI GAWTC 5 cut(s) 797, 807, 1270, 1293, 1543
Tru1I TTAA 3 cut(s) 855, 996, 1062
Tru9I TTAA 3 cut(s) 855, 996, 1062
TscAI CASTG 2 cut(s) 126, 1486
TseI GCWGC 5 cut(s) 44, 478, 641, 1214, 1484
TspDTI ATGAA 5 cut(s) 300, 584, 764, 810, 1285
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 2 cut(s) 126, 1486
Van91I CCANNNNNTGG 1 cut(s) 1442
VpaK11BI GGWCC 4 cut(s) 172, 862, 881, 1445
XapI RAATTY 7 cut(s) 356, 393, 590, 833, 941, 1018, 1490
XceI RCATGY 1 cut(s) 634
XmiI GTMKAC 3 cut(s) 247, 459, 1030
XspI CTAG 1 cut(s) 344
ZrmI AGTACT 3 cut(s) 746, 790, 910
Zsp2I ATGCAT 2 cut(s) 505, 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.