Rh2CG039600

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000523
Physical Location & Seq
Forward (+)
3148043 .. 3150145
2103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG039600.1

Sequence Viewer

Length: 2103 bp
ATGATCAACATCTACGCCATAGGGGATTTACAAATCCGAAAAGTAGTTTTGAAAGTGAGAAGATCGATGAGGAATTCAGGTGGGTGGATTCTCGCGACGTTATTATTTTTGGGTGGTTTTTTCTTTGTTCAGGCTGCGGCAAGTTCACCGAGAAGTCATCGACTACTGGCGCAAGATGCGGTCGAGATCAAGATGGTAAAAAATGGAGTTGTGTTGGATAATAAGCTTGTTCAACTAACTTTTTCTAATCCTGGTGGGGATGTTATTGGAATAAGCTATGGAGGAATTGACAACTTACTTGAAATTAACAACAAGGAAGGTAATAGAGGGTACTGGGATGTGGTTTGGAATGACCCGGGAGAGACGGAAAACACACAATACAGACTACAAGGAACACAATTTAAAGTAATCACGGAGAGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAATGCCAAACTCCAGCGGGGTGTGACAGTTCCGGTGAATGTTGACAGAAGATTCATAATGCAGCAAGGACGTTCCGGGTTCTATACATATGCCATCTTCGAGCGCCTTGAAGGGTGGCCAGGAGGGGACATGGACACAGTTAGAGTGGCTTTCAAGCTTCAGCAAGCCAAGTTTCGATATATGGCGCTATCGGACACTAGGCAAAGGTTCATGCCAACAGCCGATGACCGAGCACGCGGTAGGCCACTTGCATTCAAAGAAGCTGTTCTACTGAACAATGAGACGAGCAATCCTGAATTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCACCACCGGTGGGATTTTGGATGATCACACCAAGTGCTGAGTTTCGTATGGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTGCCTAACTGGGTTTGCTTCTCATCATTATGTTGGTAAAGATGTTGGGCTGAAATTCCAAGAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTTTATCTATCTTAACTCGGCAGCACGAAACTCAGCTAATAATCCTTCAACGATCCGTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCGTACAATTTCATTAGTTCTCCAGACTTTCCTTCTTCGGATCAACGTGGATCAGTTTCGGGCCAGTTACTAGTACATGATCGATACATTGAAAGTCTTCCAAGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAGGGAAAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTGGTTGAAAATATTAGACCCGGTAACTATAATTTGTATGCATCTGTTCCAGGGATAGTTGGAGAGTACAAATATGAGGTTGAAATTACAATTGAACCAGGAAGTAAAATCCAATTGGCTAGTATTACTTACAAACCTCCGAGAACCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCGGAGTTCTACATTCCGGATCCATATCCAACTCTCATGAACAAGTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAGTACGGGTTGTGGTCACATTATTATGATCGTTACCCTAACAATGACCTCATCTACCGTGTTGGTGCTAACAATTACCGTGAGGATTGGTTTTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGTATGTAGGAACTACATGGCAGATACAGTTTGAACTCAAGAATGCGGTCAATCCCGGAAACTATACACTTCAACTGGCCTTGGCATCAGCCACTTATGCAGAATTGCAAGTTCGGGTTAACAATTTGAATGCCAAGCCACCTCTTTTTTCGACTGGGCTAATAGGCAGCGACAATGCTATAGCAAGAGTTGGAATTCATGGCTTATACTGGTTATGGAGTATTGATGTACCAAGCACTCTACTACGCGAAGGAAGCAACACCATTTATCTAACTCAGTCCAGAGATGGCAATTCACCTTTCCACGGAGTCATGTATGATTATATAAGACTAGAAGGACCTTCTGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

700

Amino Acids

79.35

Weight (kDa)

7.68

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 57 - 256 2.1e-74 Rhamnogalacturonate lyase family
fn3_3 PF14686 419 - 490 8.3e-23 Polysaccharide lyase family 4, domain II
CBM-like PF14683 503 - 693 4.8e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 95, 693, 1995
AccIII TCCGGA 1 cut(s) 1558
AciI CCGC 6 cut(s) 137, 179, 472, 693, 1544, 1793
AclWI GGATC 6 cut(s) 1078, 1188, 1198, 1297, 1556, 1569
AcoI YGGCCR 4 cut(s) 572, 789, 1139, 1744
AcsI RAATTY 4 cut(s) 73, 752, 989, 1941
AcuI CTGAAG 1 cut(s) 599
AdeI CACNNNGTG 1 cut(s) 875
AfaI GTAC 9 cut(s) 332, 785, 1090, 1145, 1215, 1421, 1592, 1625, 1977
AfiI CCNNNNNNNGG 3 cut(s) 851, 1499, 2051
AgeI ACCGGT 2 cut(s) 847, 1499
AhlI ACTAGT 1 cut(s) 1210
AjnI CCWGG 5 cut(s) 250, 574, 1353, 1402, 1450
AluBI AGCT 6 cut(s) 226, 276, 613, 719, 1067, 1541
AluI AGCT 6 cut(s) 226, 276, 613, 719, 1067, 1541
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 2 cut(s) 356, 731
AlwI GGATC 6 cut(s) 1078, 1188, 1198, 1297, 1556, 1569
Ama87I CYCGRG 1 cut(s) 355
Aor13HI TCCGGA 1 cut(s) 1558
AoxI GGCC 8 cut(s) 419, 572, 698, 789, 1139, 1201, 1744, 1824
ApeKI GCWGC 6 cut(s) 134, 517, 1052, 1271, 1541, 1914
ApoI RAATTY 4 cut(s) 73, 752, 989, 1941
AsiGI ACCGGT 2 cut(s) 847, 1499
Asp700I GAANNNNTTC 2 cut(s) 720, 1236
AspLEI GCGC 3 cut(s) 172, 561, 643
AspS9I GGNCC 7 cut(s) 896, 910, 929, 1025, 1201, 1502, 2084
AsuC2I CCSGG 6 cut(s) 356, 357, 532, 1278, 1374, 1803
AsuHPI GGTGA 7 cut(s) 138, 439, 502, 834, 907, 1013, 2034
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 6 cut(s) 896, 910, 929, 1025, 1502, 2084
BalI TGGCCA 1 cut(s) 574
BamHI GGATCC 1 cut(s) 1561
BarI GAAGNNNNNNTAC 2 cut(s) 708, 740
BbsI GAAGAC 1 cut(s) 1229
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 6 cut(s) 121, 529, 1064, 1258, 1528, 1926
BccI CCATC 3 cut(s) 187, 557, 2027
BceAI ACGGC 1 cut(s) 1126
BciT130I CCWGG 5 cut(s) 252, 576, 1355, 1404, 1452
BciVI GTATCC 1 cut(s) 1307
BclI TGATCA 2 cut(s) 3, 864
BcnI CCSGG 6 cut(s) 356, 357, 532, 1278, 1374, 1803
BcoDI GTCTC 2 cut(s) 356, 731
BcuI ACTAGT 1 cut(s) 1210
BfaI CTAG 5 cut(s) 654, 1211, 1473, 1728, 2078
BfmI CTRYAG 1 cut(s) 1926
BfoI RGCGCY 2 cut(s) 562, 644
BfuI GTATCC 1 cut(s) 1307
BisI GCNGC 7 cut(s) 135, 138, 518, 1053, 1272, 1542, 1915
BlsI GCNGC 7 cut(s) 136, 139, 519, 1054, 1273, 1543, 1916
BmcAI AGTACT 1 cut(s) 785
Bme18I GGWCC 6 cut(s) 896, 910, 929, 1025, 1502, 2084
BmeT110I CYCGRG 1 cut(s) 355
BmgT120I GGNCC 7 cut(s) 896, 910, 929, 1025, 1201, 1502, 2084
BmiI GGNNCC 1 cut(s) 1563
BmrI ACTGGG 3 cut(s) 343, 954, 1911
BmsI GCATC 3 cut(s) 166, 1403, 1841
BmuI ACTGGG 3 cut(s) 343, 954, 1911
BpiI GAAGAC 1 cut(s) 1229
BpmI CTGGAG 2 cut(s) 452, 1146
BpuEI CTTGAG 2 cut(s) 1704, 1769
BpuMI CCSGG 6 cut(s) 356, 357, 532, 1278, 1374, 1803
Bsa29I ATCGAT 2 cut(s) 65, 1222
BsaAI YACGTR 1 cut(s) 1261
BsaBI GATNNNNATC 3 cut(s) 8, 777, 1566
BsaJI CCNNGG 6 cut(s) 355, 792, 1241, 1403, 1827, 2050
BsaWI WCCGGW 4 cut(s) 487, 847, 1499, 1558
Bsc4I CCNNNNNNNGG 3 cut(s) 851, 1499, 2051
Bse118I RCCGGY 3 cut(s) 847, 1499, 1746
Bse1I ACTGG 7 cut(s) 171, 338, 949, 1204, 1827, 1906, 1961
Bse8I GATNNNNATC 3 cut(s) 8, 777, 1566
BseAI TCCGGA 1 cut(s) 1558
BseBI CCWGG 5 cut(s) 252, 576, 1355, 1404, 1452
BseCI ATCGAT 2 cut(s) 65, 1222
BseDI CCNNGG 6 cut(s) 355, 792, 1241, 1403, 1827, 2050
BseGI GGATG 4 cut(s) 265, 343, 775, 867
BseJI GATNNNNATC 3 cut(s) 8, 777, 1566
BseLI CCNNNNNNNGG 3 cut(s) 851, 1499, 2051
BseMII CTCAG 4 cut(s) 870, 1077, 2036, 2082
BseNI ACTGG 7 cut(s) 171, 338, 949, 1204, 1827, 1906, 1961
BseX3I CGGCCG 1 cut(s) 789
BseXI GCAGC 6 cut(s) 121, 529, 1064, 1258, 1528, 1926
BsgI GTGCAG 1 cut(s) 1257
Bsh1236I CGCG 3 cut(s) 95, 693, 1995
Bsh1285I CGRYCG 2 cut(s) 183, 792
BshFI GGCC 8 cut(s) 421, 574, 700, 791, 1141, 1203, 1746, 1826
BshTI ACCGGT 2 cut(s) 847, 1499
BshVI ATCGAT 2 cut(s) 65, 1222
BsiEI CGRYCG 2 cut(s) 183, 792
BsiHKAI GWGCWC 1 cut(s) 691
BsiHKCI CYCGRG 1 cut(s) 355
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 3 cut(s) 851, 1499, 2051
BsmAI GTCTC 2 cut(s) 356, 731
BsmBI CGTCTC 2 cut(s) 356, 731
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 4 cut(s) 707, 1524, 1795, 1882
BsnI GGCC 8 cut(s) 421, 574, 700, 791, 1141, 1203, 1746, 1826
BsoBI CYCGRG 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 691
Bsp13I TCCGGA 1 cut(s) 1558
Bsp1407I TGTACA 1 cut(s) 1590
Bsp68I TCGCGA 1 cut(s) 95
BspACI CCGC 6 cut(s) 137, 179, 472, 693, 1544, 1793
BspANI GGCC 8 cut(s) 421, 574, 700, 791, 1141, 1203, 1746, 1826
BspCNI CTCAG 4 cut(s) 871, 1076, 2035, 2083
BspDI ATCGAT 2 cut(s) 65, 1222
BspEI TCCGGA 1 cut(s) 1558
BspFNI CGCG 3 cut(s) 95, 693, 1995
BspHI TCATGA 1 cut(s) 1578
BspLI GGNNCC 1 cut(s) 1563
BspPI GGATC 6 cut(s) 1078, 1188, 1198, 1297, 1556, 1569
BsrFI RCCGGY 3 cut(s) 847, 1499, 1746
BsrGI TGTACA 1 cut(s) 1590
BsrI ACTGG 7 cut(s) 171, 338, 949, 1204, 1827, 1906, 1961
BssAI RCCGGY 3 cut(s) 847, 1499, 1746
BssECI CCNNGG 6 cut(s) 355, 792, 1241, 1403, 1827, 2050
BssT1I CCWWGG 2 cut(s) 1241, 1827
Bst2UI CCWGG 5 cut(s) 252, 576, 1355, 1404, 1452
Bst4CI ACNGT 5 cut(s) 484, 595, 1679, 1700, 1776
BstAUI TGTACA 1 cut(s) 1590
BstBAI YACGTR 1 cut(s) 1261
BstC8I GCNNGC 2 cut(s) 621, 691
BstDEI CTNAG 4 cut(s) 879, 1063, 2022, 2091
BstDSI CCRYGG 1 cut(s) 2050
BstF5I GGATG 4 cut(s) 265, 343, 775, 867
BstFNI CGCG 3 cut(s) 95, 693, 1995
BstH2I RGCGCY 2 cut(s) 562, 644
BstHHI GCGC 3 cut(s) 172, 561, 643
BstMAI GTCTC 2 cut(s) 356, 731
BstMCI CGRYCG 2 cut(s) 183, 792
BstMWI GCNNNNNNNGC 3 cut(s) 176, 1844, 2001
BstNI CCWGG 5 cut(s) 252, 576, 1355, 1404, 1452
BstSFI CTRYAG 1 cut(s) 1926
BstSNI TACGTA 1 cut(s) 1261
BstUI CGCG 3 cut(s) 95, 693, 1995
BstV1I GCAGC 6 cut(s) 121, 529, 1064, 1258, 1528, 1926
BstV2I GAAGAC 1 cut(s) 1229
BstX2I RGATCY 1 cut(s) 1561
BstYI RGATCY 1 cut(s) 1561
BstZI CGGCCG 1 cut(s) 789
Bsu15I ATCGAT 2 cut(s) 65, 1222
BsuI GTATCC 1 cut(s) 1307
BsuRI GGCC 8 cut(s) 421, 574, 700, 791, 1141, 1203, 1746, 1826
BsuTUI ATCGAT 2 cut(s) 65, 1222
BtgI CCRYGG 1 cut(s) 2050
BtsCI GGATG 4 cut(s) 265, 343, 775, 867
BtuMI TCGCGA 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 621, 691
CciI TCATGA 1 cut(s) 1578
CfoI GCGC 3 cut(s) 172, 561, 643
Cfr10I RCCGGY 3 cut(s) 847, 1499, 1746
Cfr13I GGNCC 7 cut(s) 896, 910, 929, 1025, 1201, 1502, 2084
Cfr9I CCCGGG 1 cut(s) 355
ClaI ATCGAT 2 cut(s) 65, 1222
Csp6I GTAC 9 cut(s) 331, 784, 1089, 1144, 1214, 1420, 1591, 1624, 1976
CspAI ACCGGT 2 cut(s) 847, 1499
CviAII CATG 9 cut(s) 586, 667, 815, 1217, 1293, 1579, 1764, 1946, 2059
CviQI GTAC 9 cut(s) 331, 784, 1089, 1144, 1214, 1420, 1591, 1624, 1976
DdeI CTNAG 4 cut(s) 879, 1063, 2022, 2091
DraI TTTAAA 1 cut(s) 403
DraIII CACNNNGTG 1 cut(s) 875
EaeI YGGCCR 4 cut(s) 572, 789, 1139, 1744
EagI CGGCCG 1 cut(s) 789
EclXI CGGCCG 1 cut(s) 789
Eco105I TACGTA 1 cut(s) 1261
Eco130I CCWWGG 2 cut(s) 1241, 1827
Eco32I GATATC 1 cut(s) 805
Eco47I GGWCC 6 cut(s) 896, 910, 929, 1025, 1502, 2084
Eco52I CGGCCG 1 cut(s) 789
Eco57I CTGAAG 1 cut(s) 599
Eco88I CYCGRG 1 cut(s) 355
EcoO109I RGGNCCY 2 cut(s) 910, 2084
EcoRI GAATTC 2 cut(s) 73, 1941
EcoRII CCWGG 5 cut(s) 250, 574, 1353, 1402, 1450
EcoRV GATATC 1 cut(s) 805
EcoT14I CCWWGG 2 cut(s) 1241, 1827
EcoT22I ATGCAT 1 cut(s) 1396
ErhI CCWWGG 2 cut(s) 1241, 1827
Esp3I CGTCTC 2 cut(s) 356, 731
FaeI CATG 9 cut(s) 589, 670, 818, 1220, 1296, 1582, 1767, 1949, 2062
FalI AAGNNNNNCTT 2 cut(s) 1240, 1272
FaqI GGGAC 1 cut(s) 596
FatI CATG 9 cut(s) 585, 666, 814, 1216, 1292, 1578, 1763, 1945, 2058
FauI CCCGC 1 cut(s) 465
FauNDI CATATG 1 cut(s) 544
FbaI TGATCA 2 cut(s) 3, 864
Fnu4HI GCNGC 7 cut(s) 135, 138, 518, 1053, 1272, 1542, 1915
FokI GGATG 4 cut(s) 272, 350, 782, 874
Fsp4HI GCNGC 7 cut(s) 135, 138, 518, 1053, 1272, 1542, 1915
FspBI CTAG 5 cut(s) 654, 1211, 1473, 1728, 2078
GlaI GCGC 3 cut(s) 171, 560, 642
GluI GCNGC 7 cut(s) 135, 138, 518, 1053, 1272, 1542, 1915
GsuI CTGGAG 2 cut(s) 452, 1146
HaeII RGCGCY 2 cut(s) 562, 644
HaeIII GGCC 8 cut(s) 421, 574, 700, 791, 1141, 1203, 1746, 1826
HhaI GCGC 3 cut(s) 172, 561, 643
Hin1II CATG 9 cut(s) 589, 670, 818, 1220, 1296, 1582, 1767, 1949, 2062
Hin6I GCGC 3 cut(s) 170, 559, 641
HinP1I GCGC 3 cut(s) 170, 559, 641
HincII GTYRAC 2 cut(s) 499, 1867
HindII GTYRAC 2 cut(s) 499, 1867
HindIII AAGCTT 2 cut(s) 224, 611
HinfI GANTC 5 cut(s) 88, 507, 1327, 1600, 2055
HpaI GTTAAC 1 cut(s) 1867
HphI GGTGA 7 cut(s) 138, 439, 502, 834, 907, 1013, 2034
Hpy166II GTNNAC 4 cut(s) 146, 499, 1091, 1867
Hpy188I TCNGA 6 cut(s) 38, 649, 1025, 1180, 1494, 2092
Hpy8I GTNNAC 4 cut(s) 146, 499, 1091, 1867
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 8 cut(s) 311, 560, 752, 1086, 1182, 1991, 2075, 2097
HpyCH4III ACNGT 5 cut(s) 484, 595, 1679, 1700, 1776
HpyCH4IV ACGT 5 cut(s) 98, 526, 924, 1186, 1260
HpyCH4V TGCA 7 cut(s) 517, 707, 814, 1274, 1394, 1847, 1855
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 1844, 2001
HpyF3I CTNAG 4 cut(s) 879, 1063, 2022, 2091
HpySE526I ACGT 5 cut(s) 98, 526, 924, 1186, 1260
Hsp92II CATG 9 cut(s) 589, 670, 818, 1220, 1296, 1582, 1767, 1949, 2062
HspAI GCGC 3 cut(s) 170, 559, 641
Kpn2I TCCGGA 1 cut(s) 1558
Ksp22I TGATCA 2 cut(s) 3, 864
KspAI GTTAAC 1 cut(s) 1867
Lsp1109I GCAGC 6 cut(s) 121, 529, 1064, 1258, 1528, 1926
LweI GCATC 3 cut(s) 166, 1403, 1841
MaeI CTAG 5 cut(s) 654, 1211, 1473, 1728, 2078
MaeII ACGT 5 cut(s) 98, 526, 924, 1186, 1260
MaeIII GTNAC 6 cut(s) 478, 1206, 1376, 1635, 1652, 1723
MboII GAAGA 6 cut(s) 72, 516, 544, 1136, 1167, 1229
MfeI CAATTG 2 cut(s) 1443, 1466
MflI RGATCY 1 cut(s) 1561
MhlI GDGCHC 1 cut(s) 691
MlsI TGGCCA 1 cut(s) 574
MluNI TGGCCA 1 cut(s) 574
MlyI GAGTC 3 cut(s) 1321, 1609, 2064
MmeI TCCRAC 8 cut(s) 195, 800, 907, 1267, 1393, 1587, 1595, 1918
Mox20I TGGCCA 1 cut(s) 574
Mph1103I ATGCAT 1 cut(s) 1396
MroI TCCGGA 1 cut(s) 1558
MroXI GAANNNNTTC 2 cut(s) 720, 1236
MscI TGGCCA 1 cut(s) 574
MseI TTAA 4 cut(s) 306, 402, 1044, 1866
MslI CAYNNNNRTG 2 cut(s) 963, 1644
Msp20I TGGCCA 1 cut(s) 574
MspA1I CMGCKG 2 cut(s) 472, 1541
MunI CAATTG 2 cut(s) 1443, 1466
Mva1269I GAATGC 4 cut(s) 707, 1524, 1795, 1882
MvaI CCWGG 5 cut(s) 252, 576, 1355, 1404, 1452
MvnI CGCG 3 cut(s) 95, 693, 1995
MwoI GCNNNNNNNGC 3 cut(s) 176, 1844, 2001
NciI CCSGG 6 cut(s) 356, 357, 532, 1278, 1374, 1803
NdeI CATATG 1 cut(s) 544
NlaIII CATG 9 cut(s) 589, 670, 818, 1220, 1296, 1582, 1767, 1949, 2062
NlaIV GGNNCC 1 cut(s) 1563
NmeAIII GCCGAG 3 cut(s) 767, 817, 1028
NmuCI GTSAC 3 cut(s) 478, 1635, 1723
NruI TCGCGA 1 cut(s) 95
NsiI ATGCAT 1 cut(s) 1396
PagI TCATGA 1 cut(s) 1578
PctI GAATGC 4 cut(s) 707, 1524, 1795, 1882
PdmI GAANNNNTTC 2 cut(s) 720, 1236
PfeI GAWTC 2 cut(s) 88, 507
PfoI TCCNGGA 1 cut(s) 1801
PinAI ACCGGT 2 cut(s) 847, 1499
PkrI GCNGC 7 cut(s) 136, 139, 519, 1054, 1273, 1543, 1916
PleI GAGTC 3 cut(s) 1321, 1608, 2063
PpsI GAGTC 3 cut(s) 1321, 1608, 2063
Ppu21I YACGTR 1 cut(s) 1261
PpuMI RGGWCCY 2 cut(s) 910, 2084
Psp5II RGGWCCY 2 cut(s) 910, 2084
Psp6I CCWGG 5 cut(s) 250, 574, 1353, 1402, 1450
PspGI CCWGG 5 cut(s) 250, 574, 1353, 1402, 1450
PspN4I GGNNCC 1 cut(s) 1563
PspPI GGNCC 7 cut(s) 896, 910, 929, 1025, 1201, 1502, 2084
PspPPI RGGWCCY 2 cut(s) 910, 2084
PsrI GAACNNNNNNTAC 4 cut(s) 1489, 1521, 1574, 1606
PsuI RGATCY 1 cut(s) 1561
PvuII CAGCTG 1 cut(s) 1541
RruI TCGCGA 1 cut(s) 95
RsaI GTAC 9 cut(s) 332, 785, 1090, 1145, 1215, 1421, 1592, 1625, 1977
RsaNI GTAC 9 cut(s) 331, 784, 1089, 1144, 1214, 1420, 1591, 1624, 1976
RseI CAYNNNNRTG 2 cut(s) 963, 1644
SaqAI TTAA 4 cut(s) 306, 402, 1044, 1866
SatI GCNGC 7 cut(s) 135, 138, 518, 1053, 1272, 1542, 1915
Sau96I GGNCC 7 cut(s) 896, 910, 929, 1025, 1201, 1502, 2084
ScaI AGTACT 1 cut(s) 785
SchI GAGTC 3 cut(s) 1321, 1609, 2064
SduI GDGCHC 1 cut(s) 691
SfaNI GCATC 3 cut(s) 166, 1403, 1841
SfcI CTRYAG 1 cut(s) 1926
SgrAI CRCCGGYG 1 cut(s) 847
SinI GGWCC 6 cut(s) 896, 910, 929, 1025, 1502, 2084
SmaI CCCGGG 1 cut(s) 357
SmiMI CAYNNNNRTG 2 cut(s) 963, 1644
SmlI CTYRAG 2 cut(s) 1719, 1784
SmoI CTYRAG 2 cut(s) 1719, 1784
SnaBI TACGTA 1 cut(s) 1261
SpeI ACTAGT 1 cut(s) 1210
SsiI CCGC 6 cut(s) 137, 179, 472, 693, 1544, 1793
SspI AATATT 1 cut(s) 1366
SspMI CTAG 5 cut(s) 654, 1211, 1473, 1728, 2078
StyI CCWWGG 2 cut(s) 1241, 1827
TaaI ACNGT 5 cut(s) 484, 595, 1679, 1700, 1776
TaiI ACGT 5 cut(s) 101, 529, 927, 1189, 1263
TaqI TCGA 7 cut(s) 65, 160, 183, 555, 631, 1222, 1898
TaqII GACCGA 1 cut(s) 699
TatI WGTACW 4 cut(s) 783, 1213, 1419, 1590
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 2 cut(s) 88, 507
Tru1I TTAA 4 cut(s) 306, 402, 1044, 1866
Tru9I TTAA 4 cut(s) 306, 402, 1044, 1866
TseFI GTSAC 3 cut(s) 478, 1635, 1723
TseI GCWGC 6 cut(s) 134, 517, 1052, 1271, 1541, 1914
Tsp45I GTSAC 3 cut(s) 478, 1635, 1723
TspDTI ATGAA 7 cut(s) 433, 499, 655, 1141, 1353, 1595, 1934
TspGWI ACGGA 4 cut(s) 380, 428, 1076, 2067
TspMI CCCGGG 1 cut(s) 355
VpaK11BI GGWCC 6 cut(s) 896, 910, 929, 1025, 1502, 2084
XapI RAATTY 4 cut(s) 73, 752, 989, 1941
XmaI CCCGGG 1 cut(s) 355
XmnI GAANNNNTTC 2 cut(s) 720, 1236
XspI CTAG 5 cut(s) 654, 1211, 1473, 1728, 2078
ZrmI AGTACT 1 cut(s) 785
Zsp2I ATGCAT 1 cut(s) 1396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.