Rroxscaffold_2G00133420

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000523
Physical Location & Seq
Reverse (-)
70701494 .. 70706874
5381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00133420.1

Sequence Viewer

Length: 1869 bp
ATGGCATTGTCAAAGTCATTTTCTCTAATCCGGGCGGATACGTTATTGGTATACAGTACAGAGGCCTTGACATACTTGAAATGGGGAATGAAGAAAATAATAGAGGGTACTGGGATCTGGTCTGGAAAAAAGAATATAACTGACAAACTAGAAGCAACAAAATTTAGGGTTATTACCGCAAGGCCAGACCAAGCAGAAATTTCCTTCCTAAGAACATATGATGTTTCCCTTGGTAACGGCACGAACACAATTCCCTTGACCGTAGACAAAAGGTACATACTGCAGCAGGGTGGCTCTGGTTTCTATGCATATGGAATATTCGAGCGCCTTGATGGGTGGCCGGCAGTACAGGATATGGATCAAATTAGAATGGTTTACAAGCTTGAACAAGCCAAATTTCATTATATGGCGTTATCAGACGCCAGGCAAAGAAGCATGCCAACAGCAGCAGACCGTGGTCGTGGTCAGAAACTTGCGTATAAAGAAGCTTGTCTTTTGACTAATCCAAGCAATCCTGACTTTAGAGGAGAGGTAGATGACAAGTACCAGTACTCAATCGAGGACAAAGATAACAAAGTTCATGGTTGGATTAGTACTGATGAATCTGTGGGATTCTGGATGATCACACCCAGCGACGAATTTCGTACAGGAGGCCCCTTTAAGCAGGACCTCACCTCCCATGTTGGTCCAACCACCCTCTCCATGTTTGTTAGTACTCATTATGCTGGGAAGGAGCTTCAGATCAAATTTGAAGAAGGTGAGGCATGGAAAAAGGTTTTTGGGCCTGTCTTTGTGTATCTTAATTCAGCTCCAAGTTCAAAGAATTCCATCCGTATACTTTGGAATGATGCTAAAAAGCAGATGCTTAAGGAAGTCAAGAACTGGCCGTATAATTTCACTCAATCCGAGGACTTTCCTTCTTCCCATCAACGGGGATCAGTTTTGGGCCAATTACTAATACATGATCGATACATCAATGAGAGCCTTTTCCCAGCAAGTTCTGCTTATGTGGGATTGGCTGCACCCGGAGAAGTGGGATCATGGCAAAGGGAAAACAAGGGCTATCAATTTTGGAATCAAGCTGACGAAGAAGGTAGATTCATTATCAAAAATGTTCGACCCGGAAACTATAGTTTTTATGCATGGGTTCCTGGCATTGTTGGGGATTACAAATATGAGGTTGATATTACAATTACTCCAGGGTCTAATATCAAATTGGACAATCTTACTTACGAACCTCCAAGAAATGGTCCTACTTTGTGGGAAATTGGCATCCCTGATCGCATTGCTGCCGAATTCTACGTACCAGATCCTTATCCAACTCTTCTGAACAATTTATACAGCAACCAGAATCACTCAGAAAATTTTAGGCAATACGGGTTGTGGGCACGTTATGGAGAGCGATACCCTCATAATGATCTTGTGTACACCGTAGCTATCAACAATTACCATGATGATTGGTTCTATGCTCAAGTGACCAGAAATACTGGAAATGAAACCTATGTAGGAACGACGTGGCAAATTCTATTTGAACTTCATAATGTCACAAATCCAGGAAATTATACACTCCAATTGGCATTGGCCTCAGCAAACAATGCAGAGTTGCAGGTTCGAATTAACAACAAGAGCGATGAGCAACCTCATTTTTCATCAAAGTTAATAGGAGGGGACAATGCGATAGCAAGACATGGAATTCATGGTTTGTACTGGTTGTTTAGTGTTGAGATACCAAGCTCGCTACTAAAAGAAGGAATCAACACCGTCTATCTTACTCAAGCGAGAGGTGGTGAAACTTTCCAAGGAGTCATGTATGACTACATCAGACTAGAAGCACCTTCATCACACACTGATTCACAAGTAGTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

622

Amino Acids

70.83

Weight (kDa)

6.01

Isoelectric Point (pI)

32.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 42 - 178 1.8e-51 Rhamnogalacturonate lyase family
fn3_3 PF14686 333 - 406 4.3e-25 Polysaccharide lyase family 4, domain II
CBM-like PF14683 419 - 609 2e-48 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1597
AccB7I CCANNNNNTGG 1 cut(s) 1247
AccI GTMKAC 3 cut(s) 51, 264, 835
AciI CCGC 2 cut(s) 35, 177
AclWI GGATC 5 cut(s) 122, 366, 943, 1045, 1304
AcoI YGGCCR 2 cut(s) 338, 884
AcuI CTGAAG 1 cut(s) 722
AcyI GRCGYC 1 cut(s) 420
AfiI CCNNNNNNNGG 3 cut(s) 930, 931, 1247
AflII CTTAAG 1 cut(s) 866
AgsI TTSAA 5 cut(s) 79, 386, 752, 819, 1532
AjiI CACGTC 1 cut(s) 1515
AjnI CCWGG 4 cut(s) 422, 1150, 1198, 1552
AluBI AGCT 7 cut(s) 382, 488, 736, 809, 1082, 1436, 1734
AluI AGCT 7 cut(s) 382, 488, 736, 809, 1082, 1436, 1734
AlwI GGATC 5 cut(s) 122, 366, 943, 1045, 1304
AoxI GGCC 8 cut(s) 63, 182, 338, 652, 782, 884, 946, 1581
ApeKI GCWGC 4 cut(s) 283, 446, 1019, 1289
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 6 cut(s) 653, 667, 686, 782, 946, 1250
AsuC2I CCSGG 3 cut(s) 32, 1026, 1122
AsuHPI GGTGA 3 cut(s) 664, 770, 1799
AsuII TTCGAA 1 cut(s) 1612
AvaII GGWCC 3 cut(s) 667, 686, 1250
BaeGI GKGCMC 1 cut(s) 1390
BbvCI CCTCAGC 1 cut(s) 1585
BbvI GCAGC 4 cut(s) 295, 458, 1006, 1276
BccI CCATC 3 cut(s) 326, 836, 933
BceAI ACGGC 2 cut(s) 253, 871
BciT130I CCWGG 4 cut(s) 424, 1152, 1200, 1554
BciVI GTATCC 1 cut(s) 31
BclI TGATCA 1 cut(s) 621
BcnI CCSGG 3 cut(s) 32, 1026, 1122
BfaI CTAG 2 cut(s) 149, 1826
BfmI CTRYAG 2 cut(s) 281, 1129
BfoI RGCGCY 1 cut(s) 328
BfrI CTTAAG 1 cut(s) 866
BfuAI ACCTGC 1 cut(s) 1597
BfuI GTATCC 1 cut(s) 31
BisI GCNGC 4 cut(s) 284, 447, 1020, 1290
BlsI GCNGC 4 cut(s) 285, 448, 1021, 1291
BmcAI AGTACT 3 cut(s) 551, 595, 715
Bme1390I CCNGG 7 cut(s) 32, 424, 1026, 1122, 1152, 1200, 1554
Bme18I GGWCC 3 cut(s) 667, 686, 1250
BmgBI CACGTC 1 cut(s) 1515
BmgT120I GGNCC 6 cut(s) 653, 667, 686, 782, 946, 1250
BmiI GGNNCC 2 cut(s) 655, 1149
BmrFI CCNGG 7 cut(s) 32, 424, 1026, 1122, 1152, 1200, 1554
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 3 cut(s) 838, 852, 1281
BmuI ACTGGG 1 cut(s) 120
BoxI GACNNNNGTC 1 cut(s) 456
BpmI CTGGAG 1 cut(s) 1182
Bpu10I CCTNAGC 1 cut(s) 1585
Bpu14I TTCGAA 1 cut(s) 1612
BpuEI CTTGAG 2 cut(s) 1455, 1758
BpuMI CCSGG 3 cut(s) 32, 1026, 1122
Bsa29I ATCGAT 1 cut(s) 967
BsaAI YACGTR 1 cut(s) 1303
BsaBI GATNNNNATC 2 cut(s) 357, 1314
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 5 cut(s) 229, 454, 906, 1199, 1798
BsaXI ACNNNNNCTCC 4 cut(s) 659, 689, 1180, 1210
Bsc4I CCNNNNNNNGG 3 cut(s) 930, 931, 1247
Bse118I RCCGGY 1 cut(s) 340
Bse1I ACTGG 5 cut(s) 115, 547, 887, 1492, 1712
Bse3DI GCAATG 1 cut(s) 1284
Bse8I GATNNNNATC 2 cut(s) 357, 1314
BseBI CCWGG 4 cut(s) 424, 1152, 1200, 1554
BseCI ATCGAT 1 cut(s) 967
BseDI CCNNGG 5 cut(s) 229, 454, 906, 1199, 1798
BseGI GGATG 3 cut(s) 624, 828, 1272
BseJI GATNNNNATC 2 cut(s) 357, 1314
BseLI CCNNNNNNNGG 3 cut(s) 930, 931, 1247
BseMI GCAATG 1 cut(s) 1284
BseMII CTCAG 2 cut(s) 1371, 1599
BseNI ACTGG 5 cut(s) 115, 547, 887, 1492, 1712
BseRI GAGGAG 1 cut(s) 540
BseSI GKGCMC 1 cut(s) 1390
BseXI GCAGC 4 cut(s) 295, 458, 1006, 1276
BseYI CCCAGC 3 cut(s) 629, 725, 991
BsgI GTGCAG 1 cut(s) 1005
BshFI GGCC 8 cut(s) 65, 184, 340, 654, 784, 886, 948, 1583
BshVI ATCGAT 1 cut(s) 967
BsiSI CCGG 4 cut(s) 31, 341, 1026, 1122
BslFI GGGAC 1 cut(s) 1682
BslI CCNNNNNNNGG 3 cut(s) 930, 931, 1247
BsmFI GGGAC 1 cut(s) 1682
BsnI GGCC 8 cut(s) 65, 184, 340, 654, 784, 886, 948, 1583
Bsp119I TTCGAA 1 cut(s) 1612
Bsp1286I GDGCHC 1 cut(s) 1390
Bsp1407I TGTACA 1 cut(s) 1425
BspACI CCGC 2 cut(s) 35, 177
BspANI GGCC 8 cut(s) 65, 184, 340, 654, 784, 886, 948, 1583
BspCNI CTCAG 2 cut(s) 1370, 1598
BspDI ATCGAT 1 cut(s) 967
BspLI GGNNCC 2 cut(s) 655, 1149
BspMAI CTGCAG 1 cut(s) 285
BspMI ACCTGC 1 cut(s) 1597
BspPI GGATC 5 cut(s) 122, 366, 943, 1045, 1304
BspT104I TTCGAA 1 cut(s) 1612
BspTI CTTAAG 1 cut(s) 866
BsrDI GCAATG 1 cut(s) 1284
BsrFI RCCGGY 1 cut(s) 340
BsrGI TGTACA 1 cut(s) 1425
BsrI ACTGG 5 cut(s) 115, 547, 887, 1492, 1712
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 5 cut(s) 229, 454, 906, 1199, 1798
BssNAI GTATAC 2 cut(s) 52, 836
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 2 cut(s) 229, 1798
Bst1107I GTATAC 2 cut(s) 52, 836
Bst2UI CCWGG 4 cut(s) 424, 1152, 1200, 1554
Bst4CI ACNGT 5 cut(s) 56, 262, 455, 1432, 1762
Bst6I CTCTTC 1 cut(s) 1329
BstACI GRCGYC 1 cut(s) 420
BstAFI CTTAAG 1 cut(s) 866
BstAPI GCANNNNNTGC 2 cut(s) 1001, 1595
BstAUI TGTACA 1 cut(s) 1425
BstBAI YACGTR 1 cut(s) 1303
BstBI TTCGAA 1 cut(s) 1612
BstC8I GCNNGC 3 cut(s) 342, 437, 1736
BstDEI CTNAG 3 cut(s) 209, 1357, 1585
BstDSI CCRYGG 1 cut(s) 454
BstF5I GGATG 3 cut(s) 624, 828, 1272
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 1 cut(s) 327
BstMWI GCNNNNNNNGC 2 cut(s) 1001, 1595
BstNI CCWGG 4 cut(s) 424, 1152, 1200, 1554
BstNSI RCATGY 1 cut(s) 439
BstPAI GACNNNNGTC 1 cut(s) 456
BstSCI CCNGG 7 cut(s) 30, 422, 1024, 1120, 1150, 1198, 1552
BstSFI CTRYAG 2 cut(s) 281, 1129
BstSLI GKGCMC 1 cut(s) 1390
BstSNI TACGTA 1 cut(s) 1303
BstV1I GCAGC 4 cut(s) 295, 458, 1006, 1276
BstX2I RGATCY 2 cut(s) 114, 1309
BstYI RGATCY 2 cut(s) 114, 1309
BstZ17I GTATAC 2 cut(s) 52, 836
Bsu15I ATCGAT 1 cut(s) 967
BsuI GTATCC 1 cut(s) 31
BsuRI GGCC 8 cut(s) 65, 184, 340, 654, 784, 886, 948, 1583
BsuTUI ATCGAT 1 cut(s) 967
BtgI CCRYGG 1 cut(s) 454
BtgZI GCGATG 1 cut(s) 1644
BtrI CACGTC 1 cut(s) 1515
BtsCI GGATG 3 cut(s) 624, 828, 1272
BtsIMutI CAGTG 1 cut(s) 1845
BveI ACCTGC 1 cut(s) 1597
Cac8I GCNNGC 3 cut(s) 342, 437, 1736
CfoI GCGC 1 cut(s) 327
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 6 cut(s) 653, 667, 686, 782, 946, 1250
ClaI ATCGAT 1 cut(s) 967
CseI GACGC 1 cut(s) 428
DdeI CTNAG 3 cut(s) 209, 1357, 1585
EaeI YGGCCR 2 cut(s) 338, 884
Eam1104I CTCTTC 1 cut(s) 1329
EarI CTCTTC 1 cut(s) 1329
EciI GGCGGA 1 cut(s) 50
Eco105I TACGTA 1 cut(s) 1303
Eco130I CCWWGG 2 cut(s) 229, 1798
Eco147I AGGCCT 1 cut(s) 65
Eco47I GGWCC 3 cut(s) 667, 686, 1250
Eco57I CTGAAG 1 cut(s) 722
EcoO109I RGGNCCY 2 cut(s) 653, 667
EcoRI GAATTC 3 cut(s) 823, 1295, 1692
EcoRII CCWGG 4 cut(s) 422, 1150, 1198, 1552
EcoT14I CCWWGG 2 cut(s) 229, 1798
EcoT22I ATGCAT 2 cut(s) 310, 1144
ErhI CCWWGG 2 cut(s) 229, 1798
FalI AAGNNNNNCTT 4 cut(s) 477, 509, 988, 1020
FaqI GGGAC 1 cut(s) 1682
FauNDI CATATG 2 cut(s) 217, 310
FbaI TGATCA 1 cut(s) 621
FblI GTMKAC 3 cut(s) 51, 264, 835
Fnu4HI GCNGC 4 cut(s) 284, 447, 1020, 1290
FokI GGATG 3 cut(s) 631, 815, 1259
Fsp4HI GCNGC 4 cut(s) 284, 447, 1020, 1290
FspBI CTAG 2 cut(s) 149, 1826
GlaI GCGC 1 cut(s) 326
GluI GCNGC 4 cut(s) 284, 447, 1020, 1290
GsaI CCCAGC 3 cut(s) 633, 729, 995
GsuI CTGGAG 1 cut(s) 1182
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 8 cut(s) 65, 184, 340, 654, 784, 886, 948, 1583
HapII CCGG 4 cut(s) 31, 341, 1026, 1122
HgaI GACGC 1 cut(s) 428
HhaI GCGC 1 cut(s) 327
Hin1I GRCGYC 1 cut(s) 420
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HindIII AAGCTT 2 cut(s) 380, 486
HinfI GANTC 8 cut(s) 602, 612, 1075, 1098, 1351, 1752, 1803, 1850
HpaII CCGG 4 cut(s) 31, 341, 1026, 1122
HphI GGTGA 3 cut(s) 664, 770, 1799
Hpy166II GTNNAC 6 cut(s) 52, 265, 376, 836, 1426, 1428
Hpy188I TCNGA 7 cut(s) 418, 468, 741, 907, 1329, 1360, 1823
Hpy188III TCNNGA 4 cut(s) 123, 515, 616, 877
Hpy8I GTNNAC 6 cut(s) 52, 265, 376, 836, 1426, 1428
Hpy99I CGWCG 2 cut(s) 638, 1516
HpyAV CCTTC 7 cut(s) 214, 724, 749, 927, 1085, 1742, 1845
HpyCH4III ACNGT 5 cut(s) 56, 262, 455, 1432, 1762
HpyCH4IV ACGT 4 cut(s) 41, 1302, 1390, 1514
HpyCH4V TGCA 6 cut(s) 283, 308, 1022, 1142, 1598, 1606
HpyF10VI GCNNNNNNNGC 2 cut(s) 1001, 1595
HpyF3I CTNAG 3 cut(s) 209, 1357, 1585
HpySE526I ACGT 4 cut(s) 41, 1302, 1390, 1514
Hsp92I GRCGYC 1 cut(s) 420
HspAI GCGC 1 cut(s) 325
KroI GCCGGC 1 cut(s) 340
KroNI GCCGGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 621
LmnI GCTCC 2 cut(s) 733, 814
Lsp1109I GCAGC 4 cut(s) 295, 458, 1006, 1276
LweI GCATC 3 cut(s) 838, 852, 1281
MaeI CTAG 2 cut(s) 149, 1826
MaeII ACGT 4 cut(s) 41, 1302, 1390, 1514
MaeIII GTNAC 3 cut(s) 233, 1474, 1543
MboII GAAGA 5 cut(s) 103, 764, 912, 1100, 1316
MfeI CAATTG 1 cut(s) 1571
MflI RGATCY 2 cut(s) 114, 1309
MhlI GDGCHC 1 cut(s) 1390
MlyI GAGTC 1 cut(s) 1812
MmeI TCCRAC 3 cut(s) 566, 713, 1343
Mph1103I ATGCAT 2 cut(s) 310, 1144
MroNI GCCGGC 1 cut(s) 340
MseI TTAA 5 cut(s) 660, 801, 867, 1617, 1658
MspCI CTTAAG 1 cut(s) 866
MspI CCGG 4 cut(s) 31, 341, 1026, 1122
MspR9I CCNGG 7 cut(s) 32, 424, 1026, 1122, 1152, 1200, 1554
MunI CAATTG 1 cut(s) 1571
MvaI CCWGG 4 cut(s) 424, 1152, 1200, 1554
MwoI GCNNNNNNNGC 2 cut(s) 1001, 1595
NaeI GCCGGC 1 cut(s) 342
NciI CCSGG 3 cut(s) 32, 1026, 1122
NdeI CATATG 2 cut(s) 217, 310
NgoMIV GCCGGC 1 cut(s) 340
NlaIV GGNNCC 2 cut(s) 655, 1149
NmuCI GTSAC 2 cut(s) 1474, 1543
NsiI ATGCAT 2 cut(s) 310, 1144
NspI RCATGY 1 cut(s) 439
NspV TTCGAA 1 cut(s) 1612
PaeI GCATGC 1 cut(s) 439
PceI AGGCCT 1 cut(s) 65
PdiI GCCGGC 1 cut(s) 342
PfeI GAWTC 7 cut(s) 602, 612, 1075, 1098, 1351, 1752, 1850
PflMI CCANNNNNTGG 1 cut(s) 1247
PfoI TCCNGGA 1 cut(s) 1552
PkrI GCNGC 4 cut(s) 285, 448, 1021, 1291
PleI GAGTC 1 cut(s) 1811
PpsI GAGTC 1 cut(s) 1811
Ppu21I YACGTR 1 cut(s) 1303
PpuMI RGGWCCY 1 cut(s) 667
PshAI GACNNNNGTC 1 cut(s) 456
Psp5II RGGWCCY 1 cut(s) 667
Psp6I CCWGG 4 cut(s) 422, 1150, 1198, 1552
PspFI CCCAGC 3 cut(s) 629, 725, 991
PspGI CCWGG 4 cut(s) 422, 1150, 1198, 1552
PspN4I GGNNCC 2 cut(s) 655, 1149
PspPI GGNCC 6 cut(s) 653, 667, 686, 782, 946, 1250
PspPPI RGGWCCY 1 cut(s) 667
PsrI GAACNNNNNNTAC 2 cut(s) 1322, 1354
PstI CTGCAG 1 cut(s) 285
PsuI RGATCY 2 cut(s) 114, 1309
SaqAI TTAA 5 cut(s) 660, 801, 867, 1617, 1658
SatI GCNGC 4 cut(s) 284, 447, 1020, 1290
Sau96I GGNCC 6 cut(s) 653, 667, 686, 782, 946, 1250
ScaI AGTACT 3 cut(s) 551, 595, 715
SchI GAGTC 1 cut(s) 1812
ScrFI CCNGG 7 cut(s) 32, 424, 1026, 1122, 1152, 1200, 1554
SduI GDGCHC 1 cut(s) 1390
SfaNI GCATC 3 cut(s) 838, 852, 1281
SfcI CTRYAG 2 cut(s) 281, 1129
SfuI TTCGAA 1 cut(s) 1612
SinI GGWCC 3 cut(s) 667, 686, 1250
SmlI CTYRAG 3 cut(s) 866, 1470, 1773
SmoI CTYRAG 3 cut(s) 866, 1470, 1773
SnaBI TACGTA 1 cut(s) 1303
SphI GCATGC 1 cut(s) 439
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 2 cut(s) 35, 177
SspI AATATT 1 cut(s) 318
SspMI CTAG 2 cut(s) 149, 1826
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 7 cut(s) 30, 422, 1024, 1120, 1150, 1198, 1552
StyI CCWWGG 2 cut(s) 229, 1798
TaaI ACNGT 5 cut(s) 56, 262, 455, 1432, 1762
TaiI ACGT 4 cut(s) 44, 1305, 1393, 1517
TaqI TCGA 5 cut(s) 321, 558, 967, 1117, 1612
TatI WGTACW 7 cut(s) 56, 346, 549, 593, 713, 1425, 1704
TfiI GAWTC 7 cut(s) 602, 612, 1075, 1098, 1351, 1752, 1850
Tru1I TTAA 5 cut(s) 660, 801, 867, 1617, 1658
Tru9I TTAA 5 cut(s) 660, 801, 867, 1617, 1658
TscAI CASTG 1 cut(s) 1852
TseFI GTSAC 2 cut(s) 1474, 1543
TseI GCWGC 4 cut(s) 283, 446, 1019, 1289
Tsp45I GTSAC 2 cut(s) 1474, 1543
TspGWI ACGGA 1 cut(s) 821
TspRI CASTG 1 cut(s) 1852
Van91I CCANNNNNTGG 1 cut(s) 1247
Vha464I CTTAAG 1 cut(s) 866
VpaK11BI GGWCC 3 cut(s) 667, 686, 1250
XceI RCATGY 1 cut(s) 439
XmiI GTMKAC 3 cut(s) 51, 264, 835
XspI CTAG 2 cut(s) 149, 1826
ZrmI AGTACT 3 cut(s) 551, 595, 715
Zsp2I ATGCAT 2 cut(s) 310, 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.