Rh2CG221000

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000523
Physical Location & Seq
Forward (+)
22137163 .. 22148189
11027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG221000.1

Sequence Viewer

Length: 1695 bp
ATGGGGAATGAAGAAAATAATAGAGGGTACTGGGATGTGGTCTGGAAAAAGAATATAACTGACAAACTAGAAGCAACAAAATTTAGGGTTATTACCGCGAGGTCAGACCAAGCAGAAATTTCCTTCCTAAGAACATATGATGTTTCCCTTGGTAACGGCACGAACACAATTCCCTTGACAGTAGACAAAAGGTACATACTGCAGCGGGGTAGTTCTGGTTTCTATGCATATGGAATATTCGAGCGCCTTGATGGGTGGCCAGCGGTACAGGATATGGATCAAATTAGAATGGTTTACAAGCTTGAACAAGCCAAATTTCATTATATGGCGTTATCAGACACCAGGCAAAGAAGCATGCCAACAGCAGCAGACCGTGGTCGTGGTCAGAAACTTGCGTATAAAGAAGCTTGTCTTTTGACTAATCCAAGCAATCCTGACTTTAGAGGAGAGGTAGATGACAAGTACCAGTACTCAATCGAGGACAAAGATAACAAAGTTCATGGTTGGATTAGTACTGATGAATCTGTGGGATTCTGGATGATCACACCCAGCGACGAATTTCGTACAGGAGGCCCCTTTAAGCAGGACCTCACCTCCCATGTTGGTCCAACCACCCTCTCCATGTTTGTTAGTACTCATTATGCTGGGAAGGAACTTCAGATCAAATTTGAAGAAGGTGAGGCATGGAAAAAGGTTTTTGGGCCTGTCTTTGTGTATCTTAATTCGGCTCCAAGCTCAAAGAATTCCATCCGTATACTCTGGAATGATGCTAAAAAGCAGATGCTTAAAGAAGTCAAGAACTGGCCGTATAATTTCACTCAATCCGAGGACTTTCCTTCTTCCCATCAACGGGGATCAGTTTTGGGCCAATTACTAATACATGATCGATACATCAATGAGAGTTTTTTCCCAGCAAGTTCTGCTTATGTGGGATTGGCTGCACCCGGAGAAGTGGGATCATGGCAAAGGGAAAACAAGGGTTATCAATTTTGGAATCAAGCTGACGAAGAAGGTAGATTCATTATAAAAAATGTGCGACCCGGAAACTACAGTTTTTATGCATGGGTTCCTGGCATTGTTGGGGATTACAAATATGAGATTGATATTACAATTACTCCAGGGTCTAATATCAAATTGGACAATCTTACTTACGAACCTCCAAGAAATGGTCCTACTTTGTGGGAAATTGGCATCCCTGATCGCACTGCTGCCGAATTCTACGTACCAGATCCTTATCCAACTCTTTTGAACAATTTATACACCCAGAATCACACAGAAAATTTTAGGCAATACGGGTTGTGGGCACGTTATGGAGAGCGACACCCTCATAATGATCTTGTGTACAACGTAGCTATCAACAATTACCATGATGATTGGTTCTATGCTCAAGTGACCAGAAATACTGGAAATGAAACCTATGTAGGAACGACGTGGCAAATTCTATTTGAACTTCATAATGTCACAAATCCAGGAAATTATACACTCCAATTGGCATTGGCCTCAGCAAACAATGCAGAATTGCAGGTTCGAATTAACAACAAGAGCGATGAGCAACCTCATTTTTCATCAAAGTTAATCGGAGGGGACAATGCGATAGCAAGACATGGAATTCATGGGATTGAGGCTGAGACTGCTGCAAGGCTCGGTCTGGCCGGCTGCTGCAGACCGGTAGCTGTTGCTCGTGGAATCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

564

Amino Acids

64.28

Weight (kDa)

6.09

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 3 - 151 1.6e-59 Rhamnogalacturonate lyase family
fn3_3 PF14686 306 - 379 5.9e-25 Polysaccharide lyase family 4, domain II
CBM-like PF14683 392 - 541 2.2e-32 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1025
Acc36I ACCTGC 1 cut(s) 1513
AccB7I CCANNNNNTGG 1 cut(s) 1166
AccI GTMKAC 2 cut(s) 183, 754
AccII CGCG 1 cut(s) 98
AciI CCGC 3 cut(s) 96, 205, 263
AclWI GGATC 4 cut(s) 285, 862, 964, 1223
AcoI YGGCCR 3 cut(s) 257, 803, 1650
AcuI CTGAAG 1 cut(s) 641
AfiI CCNNNNNNNGG 3 cut(s) 849, 850, 1166
AgeI ACCGGT 1 cut(s) 1666
AgsI TTSAA 4 cut(s) 305, 671, 1249, 1448
AjiI CACGTC 1 cut(s) 1431
AjnI CCWGG 4 cut(s) 341, 1069, 1117, 1468
AluBI AGCT 6 cut(s) 301, 407, 735, 1001, 1352, 1673
AluI AGCT 6 cut(s) 301, 407, 735, 1001, 1352, 1673
Alw26I GTCTC 1 cut(s) 1622
AlwI GGATC 4 cut(s) 285, 862, 964, 1223
AoxI GGCC 7 cut(s) 257, 571, 701, 803, 865, 1497, 1650
ApeKI GCWGC 7 cut(s) 202, 365, 938, 1208, 1634, 1656, 1659
AsiGI ACCGGT 1 cut(s) 1666
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 6 cut(s) 572, 586, 605, 701, 865, 1169
AsuC2I CCSGG 2 cut(s) 945, 1041
AsuHPI GGTGA 2 cut(s) 583, 689
AsuII TTCGAA 1 cut(s) 1528
AvaII GGWCC 3 cut(s) 586, 605, 1169
BaeGI GKGCMC 1 cut(s) 1306
BalI TGGCCA 1 cut(s) 259
BauI CACGAG 1 cut(s) 1680
BbvCI CCTCAGC 1 cut(s) 1501
BbvI GCAGC 7 cut(s) 214, 377, 925, 1195, 1621, 1643, 1646
BccI CCATC 3 cut(s) 245, 755, 852
BceAI ACGGC 2 cut(s) 172, 790
BciT130I CCWGG 4 cut(s) 343, 1071, 1119, 1470
BclI TGATCA 1 cut(s) 540
BcnI CCSGG 2 cut(s) 945, 1041
BcoDI GTCTC 1 cut(s) 1622
BfaI CTAG 1 cut(s) 68
BfmI CTRYAG 3 cut(s) 200, 1048, 1660
BfoI RGCGCY 1 cut(s) 247
BfuAI ACCTGC 1 cut(s) 1513
BisI GCNGC 7 cut(s) 203, 366, 939, 1209, 1635, 1657, 1660
BlsI GCNGC 7 cut(s) 204, 367, 940, 1210, 1636, 1658, 1661
BmcAI AGTACT 3 cut(s) 470, 514, 634
Bme1390I CCNGG 6 cut(s) 343, 945, 1041, 1071, 1119, 1470
Bme18I GGWCC 3 cut(s) 586, 605, 1169
BmgBI CACGTC 1 cut(s) 1431
BmgT120I GGNCC 6 cut(s) 572, 586, 605, 701, 865, 1169
BmiI GGNNCC 3 cut(s) 574, 729, 1068
BmrFI CCNGG 6 cut(s) 343, 945, 1041, 1071, 1119, 1470
BmrI ACTGGG 1 cut(s) 40
BmsI GCATC 3 cut(s) 757, 771, 1200
BmuI ACTGGG 1 cut(s) 40
BoxI GACNNNNGTC 1 cut(s) 375
BpmI CTGGAG 1 cut(s) 1101
Bpu10I CCTNAGC 1 cut(s) 1501
Bpu14I TTCGAA 1 cut(s) 1528
BpuEI CTTGAG 1 cut(s) 1371
BpuMI CCSGG 2 cut(s) 945, 1041
Bsa29I ATCGAT 1 cut(s) 886
BsaAI YACGTR 1 cut(s) 1222
BsaBI GATNNNNATC 2 cut(s) 276, 1233
BsaJI CCNNGG 4 cut(s) 148, 373, 825, 1118
BsaWI WCCGGW 1 cut(s) 1666
BsaXI ACNNNNNCTCC 4 cut(s) 578, 608, 1099, 1129
Bsc4I CCNNNNNNNGG 3 cut(s) 849, 850, 1166
Bse118I RCCGGY 2 cut(s) 1652, 1666
Bse1I ACTGG 4 cut(s) 35, 466, 806, 1408
Bse8I GATNNNNATC 2 cut(s) 276, 1233
BseBI CCWGG 4 cut(s) 343, 1071, 1119, 1470
BseCI ATCGAT 1 cut(s) 886
BseDI CCNNGG 4 cut(s) 148, 373, 825, 1118
BseGI GGATG 4 cut(s) 40, 543, 747, 1191
BseJI GATNNNNATC 2 cut(s) 276, 1233
BseLI CCNNNNNNNGG 3 cut(s) 849, 850, 1166
BseMII CTCAG 2 cut(s) 1515, 1617
BseNI ACTGG 4 cut(s) 35, 466, 806, 1408
BseRI GAGGAG 1 cut(s) 459
BseSI GKGCMC 1 cut(s) 1306
BseXI GCAGC 7 cut(s) 214, 377, 925, 1195, 1621, 1643, 1646
BseYI CCCAGC 3 cut(s) 548, 644, 910
BsgI GTGCAG 1 cut(s) 924
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 7 cut(s) 259, 573, 703, 805, 867, 1499, 1652
BshTI ACCGGT 1 cut(s) 1666
BshVI ATCGAT 1 cut(s) 886
BsiSI CCGG 4 cut(s) 945, 1041, 1653, 1667
BslFI GGGAC 1 cut(s) 1598
BslI CCNNNNNNNGG 3 cut(s) 849, 850, 1166
BsmAI GTCTC 1 cut(s) 1622
BsmFI GGGAC 1 cut(s) 1598
BsnI GGCC 7 cut(s) 259, 573, 703, 805, 867, 1499, 1652
Bsp119I TTCGAA 1 cut(s) 1528
Bsp1286I GDGCHC 1 cut(s) 1306
Bsp1407I TGTACA 1 cut(s) 1341
Bsp143I GATC 9 cut(s) 277, 540, 660, 854, 883, 956, 1198, 1228, 1333
BspACI CCGC 3 cut(s) 96, 205, 263
BspANI GGCC 7 cut(s) 259, 573, 703, 805, 867, 1499, 1652
BspCNI CTCAG 2 cut(s) 1514, 1618
BspDI ATCGAT 1 cut(s) 886
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 3 cut(s) 574, 729, 1068
BspMAI CTGCAG 2 cut(s) 204, 1664
BspMI ACCTGC 1 cut(s) 1513
BspPI GGATC 4 cut(s) 285, 862, 964, 1223
BspT104I TTCGAA 1 cut(s) 1528
BsrFI RCCGGY 2 cut(s) 1652, 1666
BsrGI TGTACA 1 cut(s) 1341
BsrI ACTGG 4 cut(s) 35, 466, 806, 1408
BssAI RCCGGY 2 cut(s) 1652, 1666
BssECI CCNNGG 4 cut(s) 148, 373, 825, 1118
BssMI GATC 9 cut(s) 277, 540, 660, 854, 883, 956, 1198, 1228, 1333
BssNAI GTATAC 1 cut(s) 755
BssSI CACGAG 1 cut(s) 1680
BssT1I CCWWGG 1 cut(s) 148
Bst1107I GTATAC 1 cut(s) 755
Bst2BI CACGAG 1 cut(s) 1680
Bst2UI CCWGG 4 cut(s) 343, 1071, 1119, 1470
Bst4CI ACNGT 3 cut(s) 181, 374, 1052
BstAPI GCANNNNNTGC 2 cut(s) 920, 1511
BstAUI TGTACA 1 cut(s) 1341
BstBAI YACGTR 1 cut(s) 1222
BstBI TTCGAA 1 cut(s) 1528
BstC8I GCNNGC 3 cut(s) 261, 356, 1654
BstDEI CTNAG 3 cut(s) 128, 1501, 1626
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 4 cut(s) 40, 543, 747, 1191
BstFNI CGCG 1 cut(s) 98
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 9 cut(s) 280, 543, 663, 857, 886, 959, 1201, 1231, 1336
BstMAI GTCTC 1 cut(s) 1622
BstMBI GATC 9 cut(s) 277, 540, 660, 854, 883, 956, 1198, 1228, 1333
BstMWI GCNNNNNNNGC 3 cut(s) 920, 1511, 1631
BstNI CCWGG 4 cut(s) 343, 1071, 1119, 1470
BstNSI RCATGY 1 cut(s) 358
BstPAI GACNNNNGTC 1 cut(s) 375
BstSCI CCNGG 6 cut(s) 341, 943, 1039, 1069, 1117, 1468
BstSFI CTRYAG 3 cut(s) 200, 1048, 1660
BstSLI GKGCMC 1 cut(s) 1306
BstSNI TACGTA 1 cut(s) 1222
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 7 cut(s) 214, 377, 925, 1195, 1621, 1643, 1646
BstX2I RGATCY 1 cut(s) 1228
BstYI RGATCY 1 cut(s) 1228
BstZ17I GTATAC 1 cut(s) 755
Bsu15I ATCGAT 1 cut(s) 886
BsuRI GGCC 7 cut(s) 259, 573, 703, 805, 867, 1499, 1652
BsuTUI ATCGAT 1 cut(s) 886
BtgI CCRYGG 1 cut(s) 373
BtgZI GCGATG 1 cut(s) 1560
BtrI CACGTC 1 cut(s) 1431
BtsCI GGATG 4 cut(s) 40, 543, 747, 1191
BtsI GCAGTG 1 cut(s) 1203
BtsIMutI CAGTG 1 cut(s) 1203
BveI ACCTGC 1 cut(s) 1513
Cac8I GCNNGC 3 cut(s) 261, 356, 1654
CfoI GCGC 1 cut(s) 246
Cfr10I RCCGGY 2 cut(s) 1652, 1666
Cfr13I GGNCC 6 cut(s) 572, 586, 605, 701, 865, 1169
ClaI ATCGAT 1 cut(s) 886
CspAI ACCGGT 1 cut(s) 1666
DdeI CTNAG 3 cut(s) 128, 1501, 1626
DpnI GATC 9 cut(s) 279, 542, 662, 856, 885, 958, 1200, 1230, 1335
DpnII GATC 9 cut(s) 277, 540, 660, 854, 883, 956, 1198, 1228, 1333
EaeI YGGCCR 3 cut(s) 257, 803, 1650
Eco105I TACGTA 1 cut(s) 1222
Eco130I CCWWGG 1 cut(s) 148
Eco47I GGWCC 3 cut(s) 586, 605, 1169
Eco57I CTGAAG 1 cut(s) 641
EcoO109I RGGNCCY 2 cut(s) 572, 586
EcoRI GAATTC 3 cut(s) 742, 1214, 1608
EcoRII CCWGG 4 cut(s) 341, 1069, 1117, 1468
EcoT14I CCWWGG 1 cut(s) 148
EcoT22I ATGCAT 2 cut(s) 229, 1063
ErhI CCWWGG 1 cut(s) 148
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 907, 939
FaqI GGGAC 1 cut(s) 1598
FauI CCCGC 1 cut(s) 198
FauNDI CATATG 2 cut(s) 136, 229
FbaI TGATCA 1 cut(s) 540
FblI GTMKAC 2 cut(s) 183, 754
Fnu4HI GCNGC 7 cut(s) 203, 366, 939, 1209, 1635, 1657, 1660
FokI GGATG 4 cut(s) 47, 550, 734, 1178
Fsp4HI GCNGC 7 cut(s) 203, 366, 939, 1209, 1635, 1657, 1660
FspBI CTAG 1 cut(s) 68
GlaI GCGC 1 cut(s) 245
GluI GCNGC 7 cut(s) 203, 366, 939, 1209, 1635, 1657, 1660
GsaI CCCAGC 3 cut(s) 552, 648, 914
GsuI CTGGAG 1 cut(s) 1101
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 7 cut(s) 259, 573, 703, 805, 867, 1499, 1652
HapII CCGG 4 cut(s) 945, 1041, 1653, 1667
HhaI GCGC 1 cut(s) 246
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HindIII AAGCTT 2 cut(s) 299, 405
HinfI GANTC 6 cut(s) 521, 531, 994, 1017, 1267, 1686
HpaII CCGG 4 cut(s) 945, 1041, 1653, 1667
HphI GGTGA 2 cut(s) 583, 689
Hpy166II GTNNAC 4 cut(s) 184, 295, 755, 1342
Hpy188I TCNGA 6 cut(s) 106, 337, 387, 660, 826, 1580
Hpy188III TCNNGA 5 cut(s) 43, 434, 535, 760, 796
Hpy8I GTNNAC 4 cut(s) 184, 295, 755, 1342
Hpy99I CGWCG 2 cut(s) 557, 1432
HpyAV CCTTC 5 cut(s) 133, 643, 668, 846, 1004
HpyCH4III ACNGT 3 cut(s) 181, 374, 1052
HpyCH4IV ACGT 4 cut(s) 1221, 1306, 1347, 1430
HpyCH4V TGCA 8 cut(s) 202, 227, 941, 1061, 1514, 1522, 1637, 1662
HpyF10VI GCNNNNNNNGC 3 cut(s) 920, 1511, 1631
HpyF3I CTNAG 3 cut(s) 128, 1501, 1626
HpySE526I ACGT 4 cut(s) 1221, 1306, 1347, 1430
HspAI GCGC 1 cut(s) 244
KroI GCCGGC 1 cut(s) 1652
KroNI GCCGGC 1 cut(s) 1654
Ksp22I TGATCA 1 cut(s) 540
Kzo9I GATC 9 cut(s) 277, 540, 660, 854, 883, 956, 1198, 1228, 1333
LmnI GCTCC 1 cut(s) 733
Lsp1109I GCAGC 7 cut(s) 214, 377, 925, 1195, 1621, 1643, 1646
LweI GCATC 3 cut(s) 757, 771, 1200
MaeI CTAG 1 cut(s) 68
MaeII ACGT 4 cut(s) 1221, 1306, 1347, 1430
MaeIII GTNAC 3 cut(s) 152, 1390, 1459
MalI GATC 9 cut(s) 279, 542, 662, 856, 885, 958, 1200, 1230, 1335
MboI GATC 9 cut(s) 277, 540, 660, 854, 883, 956, 1198, 1228, 1333
MboII GAAGA 4 cut(s) 23, 683, 831, 1019
MfeI CAATTG 1 cut(s) 1487
MflI RGATCY 1 cut(s) 1228
MhlI GDGCHC 1 cut(s) 1306
MlsI TGGCCA 1 cut(s) 259
MluNI TGGCCA 1 cut(s) 259
MmeI TCCRAC 3 cut(s) 485, 632, 1262
Mox20I TGGCCA 1 cut(s) 259
Mph1103I ATGCAT 2 cut(s) 229, 1063
MroNI GCCGGC 1 cut(s) 1652
MscI TGGCCA 1 cut(s) 259
MseI TTAA 5 cut(s) 579, 720, 786, 1533, 1574
Msp20I TGGCCA 1 cut(s) 259
MspA1I CMGCKG 2 cut(s) 205, 263
MspI CCGG 4 cut(s) 945, 1041, 1653, 1667
MspR9I CCNGG 6 cut(s) 343, 945, 1041, 1071, 1119, 1470
MunI CAATTG 1 cut(s) 1487
MvaI CCWGG 4 cut(s) 343, 1071, 1119, 1470
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 920, 1511, 1631
NaeI GCCGGC 1 cut(s) 1654
NciI CCSGG 2 cut(s) 945, 1041
NdeI CATATG 2 cut(s) 136, 229
NdeII GATC 9 cut(s) 277, 540, 660, 854, 883, 956, 1198, 1228, 1333
NgoMIV GCCGGC 1 cut(s) 1652
NlaIV GGNNCC 3 cut(s) 574, 729, 1068
NmuCI GTSAC 2 cut(s) 1390, 1459
NsiI ATGCAT 2 cut(s) 229, 1063
NspI RCATGY 1 cut(s) 358
NspV TTCGAA 1 cut(s) 1528
PaeI GCATGC 1 cut(s) 358
PdiI GCCGGC 1 cut(s) 1654
PfeI GAWTC 6 cut(s) 521, 531, 994, 1017, 1267, 1686
PflMI CCANNNNNTGG 1 cut(s) 1166
PfoI TCCNGGA 1 cut(s) 1468
PinAI ACCGGT 1 cut(s) 1666
PkrI GCNGC 7 cut(s) 204, 367, 940, 1210, 1636, 1658, 1661
Ppu21I YACGTR 1 cut(s) 1222
PpuMI RGGWCCY 1 cut(s) 586
PshAI GACNNNNGTC 1 cut(s) 375
PsiI TTATAA 1 cut(s) 1025
Psp5II RGGWCCY 1 cut(s) 586
Psp6I CCWGG 4 cut(s) 341, 1069, 1117, 1468
PspFI CCCAGC 3 cut(s) 548, 644, 910
PspGI CCWGG 4 cut(s) 341, 1069, 1117, 1468
PspN4I GGNNCC 3 cut(s) 574, 729, 1068
PspPI GGNCC 6 cut(s) 572, 586, 605, 701, 865, 1169
PspPPI RGGWCCY 1 cut(s) 586
PsrI GAACNNNNNNTAC 2 cut(s) 1241, 1273
PstI CTGCAG 2 cut(s) 204, 1664
PsuI RGATCY 1 cut(s) 1228
SaqAI TTAA 5 cut(s) 579, 720, 786, 1533, 1574
SatI GCNGC 7 cut(s) 203, 366, 939, 1209, 1635, 1657, 1660
Sau3AI GATC 9 cut(s) 277, 540, 660, 854, 883, 956, 1198, 1228, 1333
Sau96I GGNCC 6 cut(s) 572, 586, 605, 701, 865, 1169
ScaI AGTACT 3 cut(s) 470, 514, 634
ScrFI CCNGG 6 cut(s) 343, 945, 1041, 1071, 1119, 1470
SduI GDGCHC 1 cut(s) 1306
SfaNI GCATC 3 cut(s) 757, 771, 1200
SfcI CTRYAG 3 cut(s) 200, 1048, 1660
SfuI TTCGAA 1 cut(s) 1528
SinI GGWCC 3 cut(s) 586, 605, 1169
SmlI CTYRAG 1 cut(s) 1386
SmoI CTYRAG 1 cut(s) 1386
SnaBI TACGTA 1 cut(s) 1222
SphI GCATGC 1 cut(s) 358
SsiI CCGC 3 cut(s) 96, 205, 263
SspI AATATT 1 cut(s) 237
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 6 cut(s) 341, 943, 1039, 1069, 1117, 1468
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 3 cut(s) 181, 374, 1052
TaiI ACGT 4 cut(s) 1224, 1309, 1350, 1433
TaqI TCGA 4 cut(s) 240, 477, 886, 1528
TaqII GACCGA 1 cut(s) 1634
TatI WGTACW 4 cut(s) 468, 512, 632, 1341
TfiI GAWTC 6 cut(s) 521, 531, 994, 1017, 1267, 1686
Tru1I TTAA 5 cut(s) 579, 720, 786, 1533, 1574
Tru9I TTAA 5 cut(s) 579, 720, 786, 1533, 1574
TscAI CASTG 1 cut(s) 1210
TseFI GTSAC 2 cut(s) 1390, 1459
TseI GCWGC 7 cut(s) 202, 365, 938, 1208, 1634, 1656, 1659
Tsp45I GTSAC 2 cut(s) 1390, 1459
TspDTI ATGAA 9 cut(s) 24, 308, 488, 534, 1009, 1425, 1442, 1554, 1601
TspGWI ACGGA 1 cut(s) 740
TspRI CASTG 1 cut(s) 1210
Van91I CCANNNNNTGG 1 cut(s) 1166
VpaK11BI GGWCC 3 cut(s) 586, 605, 1169
XceI RCATGY 1 cut(s) 358
XmiI GTMKAC 2 cut(s) 183, 754
XspI CTAG 1 cut(s) 68
ZrmI AGTACT 3 cut(s) 470, 514, 634
Zsp2I ATGCAT 2 cut(s) 229, 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.