Rorug01G0484200

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000523
Physical Location & Seq
Forward (+)
57090308 .. 57097780
7473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0484200.1

Sequence Viewer

Length: 2181 bp
ATGGCCTCCGATCCTGAGCAACCGGCGCGCTCTCCTCTCCGACAGGTTCAAACTCCAGAGGATGTTCAGATGCCTCTTCTTAATGGGGGCCAATTATCAGACAACTATTCTGTTCTTGCTGCAGTACTCCCATTTCTATTCCCAGCTCTTGGAGGACTACTGTATGGCTATGATATTGGTGCCACGTCTTGTGCTACAATATCCATAGAGTCGGCCACATTAAGTGGAATATCATGGTACGACTTATCATCTGTGGAAATTGGTCTTATAACCAGTGGCTCATTATATGGTGCCTTGATTGGCTCTGTGCTGGCCTTCAATATTGCAGACATCATAGGAAGGAGGTGGGAGTTGATTTCGTCCGCTTCAGTCTATCTTATTGGAGCTCTAGTAACAGCGCTAGCACCTGACTTGGCTGTTATGGTGATTGGGCGTTTTGTATTTGGTATAGGAATTGGACTGGCAATGCATGCTGCTCCAATGTATATTGCTGAGACAGCTCCAACTCAGATACGAGGTCGACTAATATCACTTAAAGAATTCTTTATAGTACTTGGCATGGTTGCGGGTTATGGAATTGGTAGTCTTTTAGTCAGCACAGTTGGTGGTTGGCGGTATATGTATGGAGCTAGTGTCCCTCTGGCAGTAATTATGGGAATTGGAATCTGGTGGCTACCAGAGTCACCTAGATGGATCCTTCTACGTGCCATCCAAGGAAAAGGAAATATGACTGATATGAAAGATACTGCAATCAGTTGCTTGTTCCGGCTTAGGGGTACGATTATTGGTGATTCAGCTCCTGCACTAGTAGATGAGATGCTTGATGAGCTTTCTTATGTTGGTGAAGAGAAAAAAGCTTCATTCTTAGAGTTGTTTCGTGGAAAATGCAAAAAAGCCCTCGTGATTGGTGCAGGATTAGTGTTGTTCCAACAGATCACAGGGCAACCAAGTGTGCTGTATTATGCTGCATCAATTTTTCAGACTGCAGGATTCTCCGCAGCATCTGATGCAACAGAGGTCTCAATTATACTTGGGTTATTCAAGTTGGTAATGACTGGACTAGCTGTGCTTGTTGTTGATAGACTTGGGAGGAGACCTCTACTACTTACAGGTGTTACTGGGATGGTCATCTCCTTATTCCTTTTGGGGTCGTATTACCTTTTCTTTGATGATACACCAGCCATAGCTGTAATTGCACTGCTGGCATATGTTGGGTGTTATCAGTTCTCCTTTGGTCCCATTGGTTGGCTCATGATATCAGAAATTTTTCCCTTGCGATTAAGAGGTCGCGGACTTAGCCTAGCAGTGCTTGTGAACTTTGCTGCAAATGCACTGGTGACATTTTCATTTTCCCCTCTAGAGGCATGGCTGGGAGCTGGGATACTGTTTTATGTATTTGGAGTAATAGCTGTGGTGAGAAATCTTTCAGGTTCAGGTGACATTGGTGGTGCCAAGGAGCCTCTTCTTAATGGGGTGCATAAAAATTCACATAACTATTCTCTTCTTCTCGCAATACTCCCATTTTTATTCCCAGCTCTTGAAGGACTAGTATATGGCTATGATATTGGTGCCACGGCTTGTGCTACAATTTCCCTAGAGTCAGCCACATTAAGTGGAGTATCATGGTATGACTTGTCATCTCTGCAAATTGGTCTCATAACTAGCGGCTCATTATCTGGTGCCTTGATTGGCTGTTTGTTGGCCTTCAATGCTGGTGACGTCTTAGGAAGGAGGTTTGAGTTGATTTCGGCTGCTGTATTGTATTCTGTTGGAGCTCTAATAACGGCATTAGCGCCTGAATTCCCTGTTATGGTGATTGGGCGCTTTCTATTTGGTATAGGGATTGGACTGGCAATGCATGCTGCCCCTATGTATATGGCTGAGACAGCTCCAAGTCAGATACGAGGTCAACTATTATCTCTAAAAGAGTTCTTTATAGTGATTGGGATGGTTGTTGGTTATGCGGTTGGTAGTGCTTTAGTGGATGTATTTGGTGGTTGGCGTTATATGTATGGAGCTAGCATCCCTTTGGCACTAATTATGGGAATTGGAATGTGGTGGCTACCGGAGTCACCTAGATGGATCCTTCTACGCGCCATACAGGGAAAAGGAAATATGCATGATTTAAAAGAGACTGCAATATGCTGCTTGTGCAGGCTTAGGATTCAGCTCCTGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

726

Amino Acids

77.49

Weight (kDa)

5.96

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 47 - 472 1.4e-88 Sugar (and other) transporter
MFS_1 PF07690 51 - 445 6.9e-32 Major Facilitator Superfamily
Sugar_tr PF00083 510 - 698 1.6e-40 Sugar (and other) transporter
MFS_1 PF07690 543 - 693 5.9e-19 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 269
AatII GACGTC 1 cut(s) 1722
AccB1I GGYRCC 5 cut(s) 179, 290, 1448, 1568, 1679
AccB7I CCANNNNNTGG 2 cut(s) 149, 1245
AccI GTMKAC 1 cut(s) 520
AccII CGCG 3 cut(s) 28, 1290, 2094
AciI CCGC 7 cut(s) 363, 566, 613, 996, 1290, 1665, 1964
AclWI GGATC 5 cut(s) 5, 688, 701, 2077, 2090
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 4 cut(s) 539, 1263, 1483, 1799
AcuI CTGAAG 1 cut(s) 351
AcyI GRCGYC 1 cut(s) 1719
AfaI GTAC 4 cut(s) 126, 239, 552, 778
AfeI AGCGCT 1 cut(s) 399
AfiI CCNNNNNNNGG 5 cut(s) 149, 772, 1245, 1360, 1810
AgsI TTSAA 5 cut(s) 50, 319, 1042, 1541, 1708
AhlI ACTAGT 2 cut(s) 805, 1546
AjiI CACGTC 1 cut(s) 186
Alw21I GWGCWC 2 cut(s) 388, 1777
Alw26I GTCTC 6 cut(s) 488, 1024, 1087, 1658, 1877, 2126
AlwI GGATC 5 cut(s) 5, 688, 701, 2077, 2090
AlwNI CAGNNNCTG 3 cut(s) 800, 1004, 2173
Aor51HI AGCGCT 1 cut(s) 399
AoxI GGCC 5 cut(s) 3, 88, 213, 312, 1701
ApeKI GCWGC 8 cut(s) 119, 473, 965, 998, 1322, 1751, 1862, 2145
ApoI RAATTY 4 cut(s) 539, 1263, 1483, 1799
Asp700I GAANNNNTTC 2 cut(s) 1266, 1423
AspLEI GCGC 6 cut(s) 28, 30, 400, 1795, 1824, 2096
AspS9I GGNCC 2 cut(s) 88, 1235
AsuNHI GCTAGC 2 cut(s) 400, 2018
AvaII GGWCC 1 cut(s) 1235
BamHI GGATCC 2 cut(s) 693, 2082
BanI GGYRCC 5 cut(s) 179, 290, 1448, 1568, 1679
BanII GRGCYC 2 cut(s) 388, 1777
BauI CACGAG 1 cut(s) 899
Bbv12I GWGCWC 2 cut(s) 388, 1777
BbvI GCAGC 8 cut(s) 106, 460, 952, 1010, 1309, 1738, 1849, 2132
BccI CCATC 5 cut(s) 684, 716, 1117, 1942, 2073
BceAI ACGGC 2 cut(s) 1590, 1800
BciVI GTATCC 1 cut(s) 1374
BcoDI GTCTC 6 cut(s) 488, 1024, 1087, 1658, 1877, 2126
BcuI ACTAGT 2 cut(s) 805, 1546
BfmI CTRYAG 2 cut(s) 120, 984
BfoI RGCGCY 3 cut(s) 401, 1796, 1825
BfuI GTATCC 1 cut(s) 1374
BisI GCNGC 9 cut(s) 120, 474, 966, 999, 1323, 1666, 1752, 1863, 2146
BlsI GCNGC 9 cut(s) 121, 475, 967, 1000, 1324, 1667, 1753, 1864, 2147
BmcAI AGTACT 2 cut(s) 126, 552
Bme18I GGWCC 1 cut(s) 1235
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 2 cut(s) 88, 1235
BmrI ACTGGG 1 cut(s) 1128
BmsI GCATC 6 cut(s) 60, 807, 977, 997, 1010, 2031
BmtI GCTAGC 2 cut(s) 404, 2022
BmuI ACTGGG 1 cut(s) 1128
BplI GAGNNNNNCTC 2 cut(s) 1081, 1113
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 3 cut(s) 15, 770, 2159
BsaAI YACGTR 1 cut(s) 704
BsaBI GATNNNNATC 1 cut(s) 1127
BsaHI GRCGYC 1 cut(s) 1719
BsaI GGTCTC 3 cut(s) 1024, 1087, 1658
BsaJI CCNNGG 3 cut(s) 712, 1452, 1572
BsaWI WCCGGW 1 cut(s) 2065
BsaXI ACNNNNNCTCC 4 cut(s) 375, 405, 618, 648
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 772, 1245, 1360, 1810
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 6 cut(s) 273, 465, 1060, 1123, 1338, 1854
Bse3DI GCAATG 2 cut(s) 471, 1860
Bse8I GATNNNNATC 1 cut(s) 1127
BseDI CCNNGG 3 cut(s) 712, 1452, 1572
BseGI GGATG 6 cut(s) 67, 708, 1128, 1953, 1990, 2022
BseJI GATNNNNATC 1 cut(s) 1127
BseLI CCNNNNNNNGG 5 cut(s) 149, 772, 1245, 1360, 1810
BseMI GCAATG 2 cut(s) 471, 1860
BseMII CTCAG 4 cut(s) 6, 483, 521, 1872
BseNI ACTGG 6 cut(s) 273, 465, 1060, 1123, 1338, 1854
BsePI GCGCGC 1 cut(s) 26
BseRI GAGGAG 2 cut(s) 24, 1105
BseXI GCAGC 8 cut(s) 106, 460, 952, 1010, 1309, 1738, 1849, 2132
BseYI CCCAGC 4 cut(s) 142, 1369, 1376, 1531
BsgI GTGCAG 4 cut(s) 786, 930, 2159, 2173
Bsh1236I CGCG 3 cut(s) 28, 1290, 2094
BshFI GGCC 5 cut(s) 5, 90, 215, 314, 1703
BshNI GGYRCC 5 cut(s) 179, 290, 1448, 1568, 1679
BsiHKAI GWGCWC 2 cut(s) 388, 1777
BsiSI CCGG 3 cut(s) 23, 766, 2066
BslFI GGGAC 2 cut(s) 620, 1221
BslI CCNNNNNNNGG 5 cut(s) 149, 772, 1245, 1360, 1810
BsmAI GTCTC 6 cut(s) 488, 1024, 1087, 1658, 1877, 2126
BsmFI GGGAC 2 cut(s) 620, 1221
BsnI GGCC 5 cut(s) 5, 90, 215, 314, 1703
Bso31I GGTCTC 3 cut(s) 1024, 1087, 1658
Bsp1286I GDGCHC 2 cut(s) 388, 1777
Bsp143I GATC 4 cut(s) 10, 693, 933, 2082
BspACI CCGC 7 cut(s) 363, 566, 613, 996, 1290, 1665, 1964
BspANI GGCC 5 cut(s) 5, 90, 215, 314, 1703
BspCNI CTCAG 4 cut(s) 7, 484, 520, 1873
BspFNI CGCG 3 cut(s) 28, 1290, 2094
BspHI TCATGA 1 cut(s) 1251
BspMAI CTGCAG 2 cut(s) 124, 988
BspOI GCTAGC 2 cut(s) 404, 2022
BspPI GGATC 5 cut(s) 5, 688, 701, 2077, 2090
BspT107I GGYRCC 5 cut(s) 179, 290, 1448, 1568, 1679
BspTNI GGTCTC 3 cut(s) 1024, 1087, 1658
BsrDI GCAATG 2 cut(s) 471, 1860
BsrFI RCCGGY 1 cut(s) 22
BsrI ACTGG 6 cut(s) 273, 465, 1060, 1123, 1338, 1854
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 3 cut(s) 712, 1452, 1572
BssHII GCGCGC 1 cut(s) 26
BssMI GATC 4 cut(s) 10, 693, 933, 2082
BssNI GRCGYC 1 cut(s) 1719
BssSI CACGAG 1 cut(s) 899
BssT1I CCWWGG 2 cut(s) 712, 1452
Bst2BI CACGAG 1 cut(s) 899
Bst4CI ACNGT 3 cut(s) 162, 601, 1386
Bst6I CTCTTC 4 cut(s) 81, 840, 1467, 1506
BstACI GRCGYC 1 cut(s) 1719
BstAPI GCANNNNNTGC 3 cut(s) 470, 1007, 1859
BstBAI YACGTR 1 cut(s) 704
BstC8I GCNNGC 8 cut(s) 28, 312, 402, 471, 1203, 1860, 2020, 2156
BstDEI CTNAG 9 cut(s) 15, 492, 507, 770, 865, 1295, 1723, 1881, 2159
BstDSI CCRYGG 1 cut(s) 1572
BstF5I GGATG 6 cut(s) 67, 708, 1128, 1953, 1990, 2022
BstFNI CGCG 3 cut(s) 28, 1290, 2094
BstH2I RGCGCY 3 cut(s) 401, 1796, 1825
BstHHI GCGC 6 cut(s) 28, 30, 400, 1795, 1824, 2096
BstKTI GATC 4 cut(s) 13, 696, 936, 2085
BstMAI GTCTC 6 cut(s) 488, 1024, 1087, 1658, 1877, 2126
BstMBI GATC 4 cut(s) 10, 693, 933, 2082
BstNSI RCATGY 2 cut(s) 473, 1862
BstSFI CTRYAG 2 cut(s) 120, 984
BstUI CGCG 3 cut(s) 28, 1290, 2094
BstV1I GCAGC 8 cut(s) 106, 460, 952, 1010, 1309, 1738, 1849, 2132
BstX2I RGATCY 2 cut(s) 693, 2082
BstYI RGATCY 2 cut(s) 693, 2082
BsuI GTATCC 1 cut(s) 1374
BsuRI GGCC 5 cut(s) 5, 90, 215, 314, 1703
BtgI CCRYGG 1 cut(s) 1572
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 6 cut(s) 67, 708, 1128, 1953, 1990, 2022
BtsI GCAGTG 2 cut(s) 1196, 1311
BtsIMutI CAGTG 4 cut(s) 280, 1196, 1311, 1331
Cac8I GCNNGC 8 cut(s) 28, 312, 402, 471, 1203, 1860, 2020, 2156
CaiI CAGNNNCTG 3 cut(s) 800, 1004, 2173
CciI TCATGA 1 cut(s) 1251
CfoI GCGC 6 cut(s) 28, 30, 400, 1795, 1824, 2096
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 2 cut(s) 88, 1235
Csp6I GTAC 4 cut(s) 125, 238, 551, 777
CviAII CATG 8 cut(s) 234, 470, 559, 1252, 1365, 1623, 1859, 2120
CviQI GTAC 4 cut(s) 125, 238, 551, 777
DdeI CTNAG 9 cut(s) 15, 492, 507, 770, 865, 1295, 1723, 1881, 2159
DpnI GATC 4 cut(s) 12, 695, 935, 2084
DpnII GATC 4 cut(s) 10, 693, 933, 2082
DraI TTTAAA 1 cut(s) 2127
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 4 cut(s) 81, 840, 1467, 1506
EarI CTCTTC 4 cut(s) 81, 840, 1467, 1506
Ecl136II GAGCTC 2 cut(s) 386, 1775
Eco130I CCWWGG 2 cut(s) 712, 1452
Eco24I GRGCYC 2 cut(s) 388, 1777
Eco31I GGTCTC 3 cut(s) 1024, 1087, 1658
Eco32I GATATC 1 cut(s) 1257
Eco47I GGWCC 1 cut(s) 1235
Eco47III AGCGCT 1 cut(s) 399
Eco53kI GAGCTC 2 cut(s) 386, 1775
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 2 cut(s) 386, 1775
EcoRI GAATTC 2 cut(s) 539, 1799
EcoRV GATATC 1 cut(s) 1257
EcoT14I CCWWGG 2 cut(s) 712, 1452
EcoT22I ATGCAT 3 cut(s) 471, 1860, 2121
EcoT38I GRGCYC 2 cut(s) 388, 1777
ErhI CCWWGG 2 cut(s) 712, 1452
FaeI CATG 8 cut(s) 237, 473, 562, 1255, 1368, 1626, 1862, 2123
FaqI GGGAC 2 cut(s) 620, 1221
FatI CATG 8 cut(s) 233, 469, 558, 1251, 1364, 1622, 1858, 2119
FauI CCCGC 1 cut(s) 559
FauNDI CATATG 1 cut(s) 1207
FblI GTMKAC 1 cut(s) 520
Fnu4HI GCNGC 9 cut(s) 120, 474, 966, 999, 1323, 1666, 1752, 1863, 2146
FokI GGATG 6 cut(s) 74, 695, 1135, 1960, 1997, 2009
FriOI GRGCYC 2 cut(s) 388, 1777
Fsp4HI GCNGC 9 cut(s) 120, 474, 966, 999, 1323, 1666, 1752, 1863, 2146
GlaI GCGC 6 cut(s) 27, 29, 399, 1794, 1823, 2095
GluI GCNGC 9 cut(s) 120, 474, 966, 999, 1323, 1666, 1752, 1863, 2146
GsaI CCCAGC 4 cut(s) 146, 1373, 1380, 1535
GsuI CTGGAG 1 cut(s) 39
HaeII RGCGCY 3 cut(s) 401, 1796, 1825
HaeIII GGCC 5 cut(s) 5, 90, 215, 314, 1703
HapII CCGG 3 cut(s) 23, 766, 2066
HhaI GCGC 6 cut(s) 28, 30, 400, 1795, 1824, 2096
Hin1I GRCGYC 1 cut(s) 1719
Hin1II CATG 8 cut(s) 237, 473, 562, 1255, 1368, 1626, 1862, 2123
Hin6I GCGC 6 cut(s) 26, 28, 398, 1793, 1822, 2094
HinP1I GCGC 6 cut(s) 26, 28, 398, 1793, 1822, 2094
HincII GTYRAC 2 cut(s) 521, 1910
HindII GTYRAC 2 cut(s) 521, 1910
HindIII AAGCTT 1 cut(s) 855
HinfI GANTC 8 cut(s) 209, 663, 680, 791, 990, 1598, 2069, 2164
HpaII CCGG 3 cut(s) 23, 766, 2066
Hpy166II GTNNAC 3 cut(s) 521, 1315, 1910
Hpy188I TCNGA 9 cut(s) 10, 41, 69, 100, 510, 981, 1006, 1261, 1899
Hpy188III TCNNGA 6 cut(s) 14, 56, 901, 1252, 1358, 1538
Hpy8I GTNNAC 3 cut(s) 521, 1315, 1910
HpyAV CCTTC 7 cut(s) 325, 333, 707, 1535, 1714, 1722, 2096
HpyCH4III ACNGT 3 cut(s) 162, 601, 1386
HpyCH4IV ACGT 3 cut(s) 185, 703, 1719
HpyF3I CTNAG 9 cut(s) 15, 492, 507, 770, 865, 1295, 1723, 1881, 2159
HpySE526I ACGT 3 cut(s) 185, 703, 1719
Hsp92I GRCGYC 1 cut(s) 1719
Hsp92II CATG 8 cut(s) 237, 473, 562, 1255, 1368, 1626, 1862, 2123
HspAI GCGC 6 cut(s) 26, 28, 398, 1793, 1822, 2094
Kzo9I GATC 4 cut(s) 10, 693, 933, 2082
Lsp1109I GCAGC 8 cut(s) 106, 460, 952, 1010, 1309, 1738, 1849, 2132
LweI GCATC 6 cut(s) 60, 807, 977, 997, 1010, 2031
MaeII ACGT 3 cut(s) 185, 703, 1719
MaeIII GTNAC 7 cut(s) 391, 681, 1114, 1336, 1436, 1715, 2070
MalI GATC 4 cut(s) 12, 695, 935, 2084
MboI GATC 4 cut(s) 10, 693, 933, 2082
MboII GAAGA 5 cut(s) 68, 857, 1454, 1493, 1496
MflI RGATCY 2 cut(s) 693, 2082
MhlI GDGCHC 2 cut(s) 388, 1777
MlyI GAGTC 4 cut(s) 218, 689, 1607, 2078
MmeI TCCRAC 4 cut(s) 64, 527, 952, 1750
Mph1103I ATGCAT 3 cut(s) 471, 1860, 2121
MroXI GAANNNNTTC 2 cut(s) 1266, 1423
MseI TTAA 7 cut(s) 81, 221, 534, 1280, 1467, 1610, 2126
MslI CAYNNNNRTG 2 cut(s) 688, 2077
MspI CCGG 3 cut(s) 23, 766, 2066
MvnI CGCG 3 cut(s) 28, 1290, 2094
NdeI CATATG 1 cut(s) 1207
NdeII GATC 4 cut(s) 10, 693, 933, 2082
NheI GCTAGC 2 cut(s) 400, 2018
NlaIII CATG 8 cut(s) 237, 473, 562, 1255, 1368, 1626, 1862, 2123
NmuCI GTSAC 5 cut(s) 681, 1336, 1436, 1715, 2070
NsiI ATGCAT 3 cut(s) 471, 1860, 2121
NspI RCATGY 2 cut(s) 473, 1862
PaeI GCATGC 2 cut(s) 473, 1862
PagI TCATGA 1 cut(s) 1251
PauI GCGCGC 1 cut(s) 26
PdmI GAANNNNTTC 2 cut(s) 1266, 1423
PfeI GAWTC 4 cut(s) 663, 791, 990, 2164
PflMI CCANNNNNTGG 2 cut(s) 149, 1245
PkrI GCNGC 9 cut(s) 121, 475, 967, 1000, 1324, 1667, 1753, 1864, 2147
PleI GAGTC 4 cut(s) 217, 688, 1606, 2077
PpsI GAGTC 4 cut(s) 217, 688, 1606, 2077
Ppu21I YACGTR 1 cut(s) 704
PsiI TTATAA 1 cut(s) 269
Psp124BI GAGCTC 2 cut(s) 388, 1777
PspFI CCCAGC 4 cut(s) 142, 1369, 1376, 1531
PspPI GGNCC 2 cut(s) 88, 1235
PstI CTGCAG 2 cut(s) 124, 988
PstNI CAGNNNCTG 3 cut(s) 800, 1004, 2173
PsuI RGATCY 2 cut(s) 693, 2082
PteI GCGCGC 1 cut(s) 26
RsaI GTAC 4 cut(s) 126, 239, 552, 778
RsaNI GTAC 4 cut(s) 125, 238, 551, 777
RseI CAYNNNNRTG 2 cut(s) 688, 2077
SacI GAGCTC 2 cut(s) 388, 1777
SalI GTCGAC 1 cut(s) 519
SaqAI TTAA 7 cut(s) 81, 221, 534, 1280, 1467, 1610, 2126
SatI GCNGC 9 cut(s) 120, 474, 966, 999, 1323, 1666, 1752, 1863, 2146
Sau3AI GATC 4 cut(s) 10, 693, 933, 2082
Sau96I GGNCC 2 cut(s) 88, 1235
ScaI AGTACT 2 cut(s) 126, 552
SchI GAGTC 4 cut(s) 218, 689, 1607, 2078
SduI GDGCHC 2 cut(s) 388, 1777
SfaNI GCATC 6 cut(s) 60, 807, 977, 997, 1010, 2031
SfcI CTRYAG 2 cut(s) 120, 984
SinI GGWCC 1 cut(s) 1235
SmiMI CAYNNNNRTG 2 cut(s) 688, 2077
SpeI ACTAGT 2 cut(s) 805, 1546
SphI GCATGC 2 cut(s) 473, 1862
SsiI CCGC 7 cut(s) 363, 566, 613, 996, 1290, 1665, 1964
SspI AATATT 1 cut(s) 322
SstI GAGCTC 2 cut(s) 388, 1777
StyI CCWWGG 2 cut(s) 712, 1452
TaaI ACNGT 3 cut(s) 162, 601, 1386
TaiI ACGT 3 cut(s) 188, 706, 1722
TaqI TCGA 1 cut(s) 520
TatI WGTACW 2 cut(s) 124, 550
TauI GCSGC 1 cut(s) 1668
TfiI GAWTC 4 cut(s) 663, 791, 990, 2164
Tru1I TTAA 7 cut(s) 81, 221, 534, 1280, 1467, 1610, 2126
Tru9I TTAA 7 cut(s) 81, 221, 534, 1280, 1467, 1610, 2126
TscAI CASTG 4 cut(s) 280, 1203, 1311, 1338
TseFI GTSAC 5 cut(s) 681, 1336, 1436, 1715, 2070
TseI GCWGC 8 cut(s) 119, 473, 965, 998, 1322, 1751, 1862, 2145
Tsp45I GTSAC 5 cut(s) 681, 1336, 1436, 1715, 2070
TspDTI ATGAA 3 cut(s) 752, 849, 1335
TspRI CASTG 4 cut(s) 280, 1203, 1311, 1338
Van91I CCANNNNNTGG 2 cut(s) 149, 1245
VpaK11BI GGWCC 1 cut(s) 1235
XapI RAATTY 4 cut(s) 539, 1263, 1483, 1799
XbaI TCTAGA 1 cut(s) 1357
XceI RCATGY 2 cut(s) 473, 1862
XmiI GTMKAC 1 cut(s) 520
XmnI GAANNNNTTC 2 cut(s) 1266, 1423
ZraI GACGTC 1 cut(s) 1720
ZrmI AGTACT 2 cut(s) 126, 552
Zsp2I ATGCAT 3 cut(s) 471, 1860, 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.