RLG00000015959

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000523
Physical Location & Seq
Forward (+)
2763460 .. 2765466
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015959

Sequence Viewer

Length: 2007 bp
ATGAGGAATTCAGGTGGGTGGATTTTGGGGACGTTATTATTTTTGGGTGGTTTTTTCTTTGTTCATGTTGCGGGGCAAGATGCGGTCGAGATCAAGATGGTAAAGAATGGAGTTGTGTTGGATAATAAGCTTGTTCAACTAACTTTTTCTAATCCTGGTGGGGATGTTATTGGAATAAGGTATGGAGGAATTGACAACTTACTTGAAATTAACAACAAGGAAGGTAATAGAGGGTACTGGGATGTGGTTTGGAATGACCCGGGAGAGACGGAAAACACACAATACAGACTACAAGGAACACAATTTAAAGTAATCACGGAGAGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAATGCCAACCTCCAGCGGGGTGTGACAGTTCCGGTGAATGTTGACAGAAGGTTCATAATGCAGCAAGGACGTTCCGGGTTCTATACCTATGCCATCTTCGAGCGCCTTGAAGGGTGGCCAGGAGGGGACATGGACACAGTTAGAGTGGCTTACAAGCTTCAACAAGCCAAGTTTCGATATATGGCGCTATCGGACACTAGGCAAAGGTTCATGCCAACAGCCGATGACCGAGCACGCGGTAGGCCACTTGCATACAAAGAAGCTGTTCTACTGAACAATGAGACGAGTGGTCCTGAATTTGCAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCACCACCGGTGGGATTTTGGATGATCACACCTAGTGCTGAGTATCGTATGGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTGCCTAACTGGGTTTGCTTCTCATCATTATGTTGGTAAAGATGTTGGGCTGAAATTCCAAGAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTTTATCTATCTTAACTCGGCAGCACGAAACTCAGCTAATAGTCCTTCAACGATCCGTACACTATGGAACAATGCCAAAGATCAAATGGTTGAAGAAGTCAAAAGTTGGCCATACAATTTCATTAGTTCTCCCGACTTTCCTTCTTCGGATCAACGTGGATCAGTTTCGGGCCAGTTACTAGTACATGATCGATACATTGAAAGTCTTCCAAGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGGTCATGGCAAAGGGAAAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCAATTTCCTCATTGAAAATATTAGACCCGGTAACTATAATTTGTATGCATCTGTTCCAGGGGTAGTTGGAGACTACAAATATGAGGTTGACATTACAATTAAACCTGGAAGTAAAATCCAATTGGCTAGTATTACTTACAAACCTCCGAGAACCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCGGAGTTCTACATACCGGATCCATATCCAACTCTCATGAACAAGTTGTACAAGGGGAGTCCGTTGGACAAGTTTAGGCAGTACGGGTTGTGGTCACGTTATTATGAGCGTTACCCTAACAATGACCTCATCTACCGTGTTGGTGCTGACAATTACCGTGAGGATTGGTTTTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGTATGTAGGAACTACATGGCAGATACAGTTTGAACTCAAGAATGTGGTCAATCCCGGAAACTACACACTTCAACTGGCCTTGGCATCAGCCACTTATGCAGAATTGCAAGTCCGGGTTAACAATTTGAATGCCAAGCCACCTCTTTTTTCGACAGGGCTAATAGGTGGCGACAATGCTATAGCAAGACTTGGAATTCATGGCTTATACTGGTTATGGAGTATTGATGTACCGAGCACTCTACTACGCGGAGGAAGCAACACCATTTATCTTACTCAGTCCAGAGATGGCAATTCACCTTTCCACGGAGTCATGTATGATTATATAAGACTAGAAGGACCATCGGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

669

Amino Acids

75.47

Weight (kDa)

6.5

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 22 - 224 6.8e-75 Rhamnogalacturonate lyase family
fn3_3 PF14686 387 - 458 2.2e-24 Polysaccharide lyase family 4, domain II
CBM-like PF14683 471 - 661 5.4e-48 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 597, 1899
AciI CCGC 6 cut(s) 71, 83, 376, 597, 1448, 1899
AclWI GGATC 5 cut(s) 982, 1092, 1102, 1460, 1473
AcoI YGGCCR 4 cut(s) 476, 693, 1043, 1648
AcsI RAATTY 4 cut(s) 7, 656, 893, 1845
AdeI CACNNNGTG 1 cut(s) 779
AfaI GTAC 7 cut(s) 236, 689, 994, 1119, 1496, 1529, 1881
AfiI CCNNNNNNNGG 4 cut(s) 376, 755, 1403, 1955
AgeI ACCGGT 2 cut(s) 751, 1403
AhdI GACNNNNNGTC 1 cut(s) 648
AhlI ACTAGT 1 cut(s) 1114
AjnI CCWGG 4 cut(s) 154, 478, 1306, 1354
AluBI AGCT 5 cut(s) 130, 517, 623, 971, 1445
AluI AGCT 5 cut(s) 130, 517, 623, 971, 1445
Alw21I GWGCWC 2 cut(s) 595, 1889
Alw26I GTCTC 3 cut(s) 260, 635, 1314
AlwI GGATC 5 cut(s) 982, 1092, 1102, 1460, 1473
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 8 cut(s) 323, 476, 602, 693, 1043, 1105, 1648, 1728
ApeKI GCWGC 4 cut(s) 421, 956, 1175, 1445
ApoI RAATTY 4 cut(s) 7, 656, 893, 1845
AsiGI ACCGGT 2 cut(s) 751, 1403
Asp700I GAANNNNTTC 2 cut(s) 624, 1140
AspLEI GCGC 2 cut(s) 465, 547
AspS9I GGNCC 8 cut(s) 650, 800, 814, 833, 929, 1105, 1406, 1988
AsuC2I CCSGG 7 cut(s) 260, 261, 436, 1182, 1278, 1707, 1766
AsuHPI GGTGA 6 cut(s) 343, 406, 738, 811, 917, 1938
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 7 cut(s) 650, 800, 814, 833, 929, 1406, 1988
BalI TGGCCA 2 cut(s) 478, 1045
BamHI GGATCC 1 cut(s) 1465
BarI GAAGNNNNNNTAC 2 cut(s) 612, 644
BbsI GAAGAC 1 cut(s) 1133
Bbv12I GWGCWC 2 cut(s) 595, 1889
BbvI GCAGC 4 cut(s) 433, 968, 1162, 1432
BccI CCATC 4 cut(s) 91, 461, 1931, 1999
BciT130I CCWGG 4 cut(s) 156, 480, 1308, 1356
BciVI GTATCC 1 cut(s) 1211
BclI TGATCA 1 cut(s) 768
BcnI CCSGG 7 cut(s) 260, 261, 436, 1182, 1278, 1707, 1766
BcoDI GTCTC 3 cut(s) 260, 635, 1314
BcuI ACTAGT 1 cut(s) 1114
BfaI CTAG 6 cut(s) 558, 777, 1115, 1377, 1632, 1982
BfmI CTRYAG 1 cut(s) 1830
BfoI RGCGCY 2 cut(s) 466, 548
BfuI GTATCC 1 cut(s) 1211
BisI GCNGC 4 cut(s) 422, 957, 1176, 1446
BlsI GCNGC 4 cut(s) 423, 958, 1177, 1447
BmcAI AGTACT 1 cut(s) 689
Bme18I GGWCC 7 cut(s) 650, 800, 814, 833, 929, 1406, 1988
BmeRI GACNNNNNGTC 1 cut(s) 648
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 8 cut(s) 650, 800, 814, 833, 929, 1105, 1406, 1988
BmiI GGNNCC 1 cut(s) 1467
BmrI ACTGGG 2 cut(s) 247, 858
BmsI GCATC 3 cut(s) 70, 1307, 1745
BmuI ACTGGG 2 cut(s) 247, 858
BpiI GAAGAC 1 cut(s) 1133
BpmI CTGGAG 1 cut(s) 356
BpuEI CTTGAG 2 cut(s) 1608, 1673
BpuMI CCSGG 7 cut(s) 260, 261, 436, 1182, 1278, 1707, 1766
Bsa29I ATCGAT 1 cut(s) 1126
BsaAI YACGTR 1 cut(s) 1165
BsaBI GATNNNNATC 2 cut(s) 681, 1470
BsaJI CCNNGG 6 cut(s) 259, 696, 1145, 1307, 1731, 1954
BsaWI WCCGGW 4 cut(s) 391, 751, 1403, 1462
Bsc4I CCNNNNNNNGG 4 cut(s) 376, 755, 1403, 1955
Bse118I RCCGGY 3 cut(s) 751, 1403, 1650
Bse1I ACTGG 5 cut(s) 242, 853, 1108, 1731, 1865
Bse8I GATNNNNATC 2 cut(s) 681, 1470
BseBI CCWGG 4 cut(s) 156, 480, 1308, 1356
BseCI ATCGAT 1 cut(s) 1126
BseDI CCNNGG 6 cut(s) 259, 696, 1145, 1307, 1731, 1954
BseGI GGATG 4 cut(s) 169, 247, 679, 771
BseJI GATNNNNATC 2 cut(s) 681, 1470
BseLI CCNNNNNNNGG 4 cut(s) 376, 755, 1403, 1955
BseMII CTCAG 3 cut(s) 774, 981, 1940
BseNI ACTGG 5 cut(s) 242, 853, 1108, 1731, 1865
BseX3I CGGCCG 1 cut(s) 693
BseXI GCAGC 4 cut(s) 433, 968, 1162, 1432
BsgI GTGCAG 1 cut(s) 1161
Bsh1236I CGCG 2 cut(s) 597, 1899
Bsh1285I CGRYCG 2 cut(s) 87, 696
BshFI GGCC 8 cut(s) 325, 478, 604, 695, 1045, 1107, 1650, 1730
BshTI ACCGGT 2 cut(s) 751, 1403
BshVI ATCGAT 1 cut(s) 1126
BsiEI CGRYCG 2 cut(s) 87, 696
BsiHKAI GWGCWC 2 cut(s) 595, 1889
BsiHKCI CYCGRG 1 cut(s) 259
BslFI GGGAC 2 cut(s) 43, 500
BslI CCNNNNNNNGG 4 cut(s) 376, 755, 1403, 1955
BsmAI GTCTC 3 cut(s) 260, 635, 1314
BsmBI CGTCTC 2 cut(s) 260, 635
BsmFI GGGAC 2 cut(s) 43, 500
BsmI GAATGC 2 cut(s) 1428, 1786
BsnI GGCC 8 cut(s) 325, 478, 604, 695, 1045, 1107, 1650, 1730
BsoBI CYCGRG 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 595, 1889
Bsp1407I TGTACA 1 cut(s) 1494
Bsp143I GATC 9 cut(s) 90, 768, 987, 1015, 1084, 1094, 1123, 1435, 1465
BspACI CCGC 6 cut(s) 71, 83, 376, 597, 1448, 1899
BspANI GGCC 8 cut(s) 325, 478, 604, 695, 1045, 1107, 1650, 1730
BspCNI CTCAG 3 cut(s) 775, 980, 1939
BspDI ATCGAT 1 cut(s) 1126
BspFNI CGCG 2 cut(s) 597, 1899
BspHI TCATGA 1 cut(s) 1482
BspLI GGNNCC 1 cut(s) 1467
BspPI GGATC 5 cut(s) 982, 1092, 1102, 1460, 1473
BsrFI RCCGGY 3 cut(s) 751, 1403, 1650
BsrGI TGTACA 1 cut(s) 1494
BsrI ACTGG 5 cut(s) 242, 853, 1108, 1731, 1865
BssAI RCCGGY 3 cut(s) 751, 1403, 1650
BssECI CCNNGG 6 cut(s) 259, 696, 1145, 1307, 1731, 1954
BssMI GATC 9 cut(s) 90, 768, 987, 1015, 1084, 1094, 1123, 1435, 1465
BssT1I CCWWGG 2 cut(s) 1145, 1731
Bst2UI CCWGG 4 cut(s) 156, 480, 1308, 1356
Bst4CI ACNGT 5 cut(s) 388, 499, 1583, 1604, 1680
BstAUI TGTACA 1 cut(s) 1494
BstBAI YACGTR 1 cut(s) 1165
BstC8I GCNNGC 1 cut(s) 595
BstDEI CTNAG 3 cut(s) 783, 967, 1926
BstDSI CCRYGG 1 cut(s) 1954
BstF5I GGATG 4 cut(s) 169, 247, 679, 771
BstFNI CGCG 2 cut(s) 597, 1899
BstH2I RGCGCY 2 cut(s) 466, 548
BstHHI GCGC 2 cut(s) 465, 547
BstKTI GATC 9 cut(s) 93, 771, 990, 1018, 1087, 1097, 1126, 1438, 1468
BstMAI GTCTC 3 cut(s) 260, 635, 1314
BstMBI GATC 9 cut(s) 90, 768, 987, 1015, 1084, 1094, 1123, 1435, 1465
BstMCI CGRYCG 2 cut(s) 87, 696
BstMWI GCNNNNNNNGC 2 cut(s) 1748, 1905
BstNI CCWGG 4 cut(s) 156, 480, 1308, 1356
BstSFI CTRYAG 1 cut(s) 1830
BstSNI TACGTA 1 cut(s) 1165
BstUI CGCG 2 cut(s) 597, 1899
BstV1I GCAGC 4 cut(s) 433, 968, 1162, 1432
BstV2I GAAGAC 1 cut(s) 1133
BstX2I RGATCY 1 cut(s) 1465
BstYI RGATCY 1 cut(s) 1465
BstZI CGGCCG 1 cut(s) 693
Bsu15I ATCGAT 1 cut(s) 1126
BsuI GTATCC 1 cut(s) 1211
BsuRI GGCC 8 cut(s) 325, 478, 604, 695, 1045, 1107, 1650, 1730
BsuTUI ATCGAT 1 cut(s) 1126
BtgI CCRYGG 1 cut(s) 1954
BtsCI GGATG 4 cut(s) 169, 247, 679, 771
Cac8I GCNNGC 1 cut(s) 595
CciI TCATGA 1 cut(s) 1482
CfoI GCGC 2 cut(s) 465, 547
Cfr10I RCCGGY 3 cut(s) 751, 1403, 1650
Cfr13I GGNCC 8 cut(s) 650, 800, 814, 833, 929, 1105, 1406, 1988
Cfr9I CCCGGG 1 cut(s) 259
ClaI ATCGAT 1 cut(s) 1126
Csp6I GTAC 7 cut(s) 235, 688, 993, 1118, 1495, 1528, 1880
CspAI ACCGGT 2 cut(s) 751, 1403
CviQI GTAC 7 cut(s) 235, 688, 993, 1118, 1495, 1528, 1880
DdeI CTNAG 3 cut(s) 783, 967, 1926
DpnI GATC 9 cut(s) 92, 770, 989, 1017, 1086, 1096, 1125, 1437, 1467
DpnII GATC 9 cut(s) 90, 768, 987, 1015, 1084, 1094, 1123, 1435, 1465
DraI TTTAAA 1 cut(s) 307
DraIII CACNNNGTG 1 cut(s) 779
DriI GACNNNNNGTC 1 cut(s) 648
EaeI YGGCCR 4 cut(s) 476, 693, 1043, 1648
EagI CGGCCG 1 cut(s) 693
Eam1105I GACNNNNNGTC 1 cut(s) 648
EclXI CGGCCG 1 cut(s) 693
Eco105I TACGTA 1 cut(s) 1165
Eco130I CCWWGG 2 cut(s) 1145, 1731
Eco32I GATATC 1 cut(s) 709
Eco47I GGWCC 7 cut(s) 650, 800, 814, 833, 929, 1406, 1988
Eco52I CGGCCG 1 cut(s) 693
Eco88I CYCGRG 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 814
EcoRI GAATTC 2 cut(s) 7, 1845
EcoRII CCWGG 4 cut(s) 154, 478, 1306, 1354
EcoRV GATATC 1 cut(s) 709
EcoT14I CCWWGG 2 cut(s) 1145, 1731
EcoT22I ATGCAT 1 cut(s) 1300
ErhI CCWWGG 2 cut(s) 1145, 1731
Esp3I CGTCTC 2 cut(s) 260, 635
FalI AAGNNNNNCTT 2 cut(s) 1144, 1176
FaqI GGGAC 2 cut(s) 43, 500
FauI CCCGC 2 cut(s) 64, 369
FbaI TGATCA 1 cut(s) 768
Fnu4HI GCNGC 4 cut(s) 422, 957, 1176, 1446
FokI GGATG 4 cut(s) 176, 254, 686, 778
Fsp4HI GCNGC 4 cut(s) 422, 957, 1176, 1446
FspBI CTAG 6 cut(s) 558, 777, 1115, 1377, 1632, 1982
GlaI GCGC 2 cut(s) 464, 546
GluI GCNGC 4 cut(s) 422, 957, 1176, 1446
GsuI CTGGAG 1 cut(s) 356
HaeII RGCGCY 2 cut(s) 466, 548
HaeIII GGCC 8 cut(s) 325, 478, 604, 695, 1045, 1107, 1650, 1730
HhaI GCGC 2 cut(s) 465, 547
Hin6I GCGC 2 cut(s) 463, 545
HinP1I GCGC 2 cut(s) 463, 545
HincII GTYRAC 3 cut(s) 403, 1339, 1771
HindII GTYRAC 3 cut(s) 403, 1339, 1771
HindIII AAGCTT 2 cut(s) 128, 515
HinfI GANTC 3 cut(s) 1231, 1504, 1959
HpaI GTTAAC 1 cut(s) 1771
HphI GGTGA 6 cut(s) 343, 406, 738, 811, 917, 1938
Hpy166II GTNNAC 4 cut(s) 403, 995, 1339, 1771
Hpy188I TCNGA 5 cut(s) 553, 929, 1084, 1398, 1996
Hpy188III TCNNGA 8 cut(s) 88, 94, 653, 1067, 1433, 1483, 1690, 1932
Hpy8I GTNNAC 4 cut(s) 403, 995, 1339, 1771
HpyAV CCTTC 6 cut(s) 215, 402, 464, 990, 1086, 1979
HpyCH4III ACNGT 5 cut(s) 388, 499, 1583, 1604, 1680
HpyCH4IV ACGT 6 cut(s) 32, 430, 828, 1090, 1164, 1543
HpyCH4V TGCA 8 cut(s) 421, 611, 662, 718, 1178, 1298, 1751, 1759
HpyF10VI GCNNNNNNNGC 2 cut(s) 1748, 1905
HpyF3I CTNAG 3 cut(s) 783, 967, 1926
HpySE526I ACGT 6 cut(s) 32, 430, 828, 1090, 1164, 1543
HspAI GCGC 2 cut(s) 463, 545
Ksp22I TGATCA 1 cut(s) 768
KspAI GTTAAC 1 cut(s) 1771
Kzo9I GATC 9 cut(s) 90, 768, 987, 1015, 1084, 1094, 1123, 1435, 1465
Lsp1109I GCAGC 4 cut(s) 433, 968, 1162, 1432
LweI GCATC 3 cut(s) 70, 1307, 1745
MaeI CTAG 6 cut(s) 558, 777, 1115, 1377, 1632, 1982
MaeII ACGT 6 cut(s) 32, 430, 828, 1090, 1164, 1543
MaeIII GTNAC 6 cut(s) 382, 1110, 1280, 1539, 1556, 1627
MalI GATC 9 cut(s) 92, 770, 989, 1017, 1086, 1096, 1125, 1437, 1467
MboI GATC 9 cut(s) 90, 768, 987, 1015, 1084, 1094, 1123, 1435, 1465
MboII GAAGA 4 cut(s) 448, 1040, 1071, 1133
MfeI CAATTG 1 cut(s) 1370
MflI RGATCY 1 cut(s) 1465
MhlI GDGCHC 2 cut(s) 595, 1889
MlsI TGGCCA 2 cut(s) 478, 1045
MluNI TGGCCA 2 cut(s) 478, 1045
MlyI GAGTC 3 cut(s) 1225, 1513, 1968
MmeI TCCRAC 6 cut(s) 99, 704, 811, 1297, 1491, 1499
Mox20I TGGCCA 2 cut(s) 478, 1045
Mph1103I ATGCAT 1 cut(s) 1300
MroXI GAANNNNTTC 2 cut(s) 624, 1140
MscI TGGCCA 2 cut(s) 478, 1045
MseI TTAA 5 cut(s) 210, 306, 948, 1350, 1770
MslI CAYNNNNRTG 1 cut(s) 867
Msp20I TGGCCA 2 cut(s) 478, 1045
MspA1I CMGCKG 2 cut(s) 376, 1445
MunI CAATTG 1 cut(s) 1370
Mva1269I GAATGC 2 cut(s) 1428, 1786
MvaI CCWGG 4 cut(s) 156, 480, 1308, 1356
MvnI CGCG 2 cut(s) 597, 1899
MwoI GCNNNNNNNGC 2 cut(s) 1748, 1905
NciI CCSGG 7 cut(s) 260, 261, 436, 1182, 1278, 1707, 1766
NdeII GATC 9 cut(s) 90, 768, 987, 1015, 1084, 1094, 1123, 1435, 1465
NlaIV GGNNCC 1 cut(s) 1467
NmeAIII GCCGAG 3 cut(s) 671, 721, 932
NmuCI GTSAC 3 cut(s) 382, 1539, 1627
NsiI ATGCAT 1 cut(s) 1300
PagI TCATGA 1 cut(s) 1482
PctI GAATGC 2 cut(s) 1428, 1786
PdmI GAANNNNTTC 2 cut(s) 624, 1140
PfoI TCCNGGA 1 cut(s) 1705
PinAI ACCGGT 2 cut(s) 751, 1403
PkrI GCNGC 4 cut(s) 423, 958, 1177, 1447
PleI GAGTC 3 cut(s) 1225, 1512, 1967
PpsI GAGTC 3 cut(s) 1225, 1512, 1967
Ppu21I YACGTR 1 cut(s) 1165
PpuMI RGGWCCY 1 cut(s) 814
Psp5II RGGWCCY 1 cut(s) 814
Psp6I CCWGG 4 cut(s) 154, 478, 1306, 1354
PspGI CCWGG 4 cut(s) 154, 478, 1306, 1354
PspN4I GGNNCC 1 cut(s) 1467
PspPI GGNCC 8 cut(s) 650, 800, 814, 833, 929, 1105, 1406, 1988
PspPPI RGGWCCY 1 cut(s) 814
PsrI GAACNNNNNNTAC 4 cut(s) 1393, 1425, 1478, 1510
PsuI RGATCY 1 cut(s) 1465
PvuII CAGCTG 1 cut(s) 1445
RsaI GTAC 7 cut(s) 236, 689, 994, 1119, 1496, 1529, 1881
RsaNI GTAC 7 cut(s) 235, 688, 993, 1118, 1495, 1528, 1880
RseI CAYNNNNRTG 1 cut(s) 867
SaqAI TTAA 5 cut(s) 210, 306, 948, 1350, 1770
SatI GCNGC 4 cut(s) 422, 957, 1176, 1446
Sau3AI GATC 9 cut(s) 90, 768, 987, 1015, 1084, 1094, 1123, 1435, 1465
Sau96I GGNCC 8 cut(s) 650, 800, 814, 833, 929, 1105, 1406, 1988
ScaI AGTACT 1 cut(s) 689
SchI GAGTC 3 cut(s) 1225, 1513, 1968
SduI GDGCHC 2 cut(s) 595, 1889
SfaNI GCATC 3 cut(s) 70, 1307, 1745
SfcI CTRYAG 1 cut(s) 1830
SgrAI CRCCGGYG 1 cut(s) 751
SinI GGWCC 7 cut(s) 650, 800, 814, 833, 929, 1406, 1988
SmaI CCCGGG 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 867
SmlI CTYRAG 2 cut(s) 1623, 1688
SmoI CTYRAG 2 cut(s) 1623, 1688
SnaBI TACGTA 1 cut(s) 1165
SpeI ACTAGT 1 cut(s) 1114
SsiI CCGC 6 cut(s) 71, 83, 376, 597, 1448, 1899
SspI AATATT 1 cut(s) 1270
SspMI CTAG 6 cut(s) 558, 777, 1115, 1377, 1632, 1982
StyI CCWWGG 2 cut(s) 1145, 1731
TaaI ACNGT 5 cut(s) 388, 499, 1583, 1604, 1680
TaiI ACGT 6 cut(s) 35, 433, 831, 1093, 1167, 1546
TaqI TCGA 5 cut(s) 87, 459, 535, 1126, 1802
TaqII GACCGA 1 cut(s) 603
TatI WGTACW 3 cut(s) 687, 1117, 1494
Tru1I TTAA 5 cut(s) 210, 306, 948, 1350, 1770
Tru9I TTAA 5 cut(s) 210, 306, 948, 1350, 1770
TseFI GTSAC 3 cut(s) 382, 1539, 1627
TseI GCWGC 4 cut(s) 421, 956, 1175, 1445
Tsp45I GTSAC 3 cut(s) 382, 1539, 1627
TspDTI ATGAA 8 cut(s) 53, 337, 403, 559, 1045, 1257, 1499, 1838
TspGWI ACGGA 5 cut(s) 284, 332, 980, 1497, 1971
TspMI CCCGGG 1 cut(s) 259
VpaK11BI GGWCC 7 cut(s) 650, 800, 814, 833, 929, 1406, 1988
XapI RAATTY 4 cut(s) 7, 656, 893, 1845
XcmI CCANNNNNNNNNTGG 1 cut(s) 1018
XmaI CCCGGG 1 cut(s) 259
XmnI GAANNNNTTC 2 cut(s) 624, 1140
XspI CTAG 6 cut(s) 558, 777, 1115, 1377, 1632, 1982
ZrmI AGTACT 1 cut(s) 689
Zsp2I ATGCAT 1 cut(s) 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.