Rorug02G0167600

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000523
Physical Location & Seq
Forward (+)
15060094 .. 15061743
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0167600.1

Sequence Viewer

Length: 891 bp
ATGCATATGGAGAAAGGTGCAGAGCTAGTCCGCAAGTGCTCCAAGAATGGTGTGCGCGCCAAATACAAAAAGAAGAAATACACCAACCAACTTCTTGAGTTGGATGCATGTCTTCAGAGGCAGTTGAATGTACTGAGAGTGCAGGTGGCAAGCGATGTGAGGGAGACCACAGCTTCGATAAGGAGTATACAGAAGATGATCAAGCGAATTGAGGAGAGTGGTGTCATGAAGAATCAAATGGAAATGAATGGTCCGTGTGAAGTAGTTGTCGAGGCTTCAACCCCTGATGAACAGAATGAGGTTGGGTTGGATGTGGCTTTGAAAGAGATGAAGATGAAGCTACTGGAGGATGGGTTTACGAAGATTGGTGTGTGGGGTGTTGGAGGAGTCGGGAAGACCACATTGGTGAAAATGTTGTGTCATGATCAGGAAGTCAAAGATAAATTCAAGAAAATCTTCTTTACCACTGCTTCGAAATGGCAGAACCTGAACCTCATTATGCAAGAACTATATCGAGTCCTGTGTTCCCAGGTTCCTACTTTCCAAAACAAAGAAGGGAGACGTTATCTGGTTGTCCTGGATGATGTTGGGGATGGATCAGAATCCATTCTTCCGGAGTTTGATGGATTGAAAATTCCAAATAGCAAGATTTTGATTACATCAAGATATGCATTTCCAGGAGTTGGTTCTCAATACCATTTGAAATCTCTGAACGATGAGGATGCAATGTCTCTTTTTCATCACTCAGCGTCATTGGGAGATAGGTGCTCTTGTATTCCAGAAGATCTTTTGAGAAAGATAGTAGAGTGTTGTAAGGGACTTCCACTTGCAATCACTGTGAACGGAAGATCACTTTGGGGTAACCTATTGAAATCTGGCAAAAGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

296

Amino Acids

33.33

Weight (kDa)

8.93

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 2 - 68 5.1e-06 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 107 - 249 4e-12 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
Acc36I ACCTGC 1 cut(s) 133
AccB7I CCANNNNNTGG 1 cut(s) 683
AccI GTMKAC 1 cut(s) 187
AccII CGCG 1 cut(s) 57
AccIII TCCGGA 1 cut(s) 613
AciI CCGC 1 cut(s) 31
AclWI GGATC 1 cut(s) 604
AcsI RAATTY 2 cut(s) 443, 633
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 683
AgsI TTSAA 7 cut(s) 127, 279, 322, 448, 631, 703, 871
AjnI CCWGG 3 cut(s) 528, 576, 676
AjuI GAANNNNNNNTTGG 4 cut(s) 386, 418, 838, 870
AleI CACNNNNGTG 1 cut(s) 404
AluBI AGCT 3 cut(s) 25, 173, 340
AluI AGCT 3 cut(s) 25, 173, 340
Alw21I GWGCWC 2 cut(s) 41, 770
Alw26I GTCTC 3 cut(s) 158, 553, 735
AlwI GGATC 1 cut(s) 604
AlwNI CAGNNNCTG 1 cut(s) 487
Aor13HI TCCGGA 1 cut(s) 613
ApoI RAATTY 2 cut(s) 443, 633
ArsI GACNNNNNNTTYG 2 cut(s) 157, 189
Asp700I GAANNNNTTC 2 cut(s) 455, 606
AspLEI GCGC 2 cut(s) 57, 59
AspS9I GGNCC 1 cut(s) 251
AsuHPI GGTGA 1 cut(s) 418
AsuII TTCGAA 1 cut(s) 473
AvaII GGWCC 1 cut(s) 251
BbsI GAAGAC 2 cut(s) 104, 401
Bbv12I GWGCWC 2 cut(s) 41, 770
BccI CCATC 3 cut(s) 344, 587, 617
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 3 cut(s) 530, 578, 678
BclI TGATCA 2 cut(s) 198, 424
BcoDI GTCTC 3 cut(s) 158, 553, 735
BfaI CTAG 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 133
BglII AGATCT 1 cut(s) 784
Bme1390I CCNGG 3 cut(s) 530, 578, 678
Bme18I GGWCC 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 251
BmiI GGNNCC 1 cut(s) 534
BmrFI CCNGG 3 cut(s) 530, 578, 678
BmsI GCATC 2 cut(s) 94, 712
BpiI GAAGAC 2 cut(s) 104, 401
BpmI CTGGAG 1 cut(s) 365
Bpu14I TTCGAA 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 601
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 528
BsaWI WCCGGW 1 cut(s) 613
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 683
Bse1I ACTGG 1 cut(s) 348
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 601
BseAI TCCGGA 1 cut(s) 613
BseBI CCWGG 3 cut(s) 530, 578, 678
BseDI CCNNGG 1 cut(s) 528
BseGI GGATG 6 cut(s) 109, 316, 355, 586, 598, 727
BseJI GATNNNNATC 1 cut(s) 601
BseLI CCNNNNNNNGG 1 cut(s) 683
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 2 cut(s) 125, 759
BseNI ACTGG 1 cut(s) 348
BsePI GCGCGC 1 cut(s) 55
BseRI GAGGAG 2 cut(s) 227, 399
BsgI GTGCAG 2 cut(s) 39, 161
Bsh1236I CGCG 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 41, 770
BsiSI CCGG 1 cut(s) 614
BslFI GGGAC 1 cut(s) 831
BslI CCNNNNNNNGG 1 cut(s) 683
BsmAI GTCTC 3 cut(s) 158, 553, 735
BsmBI CGTCTC 1 cut(s) 553
BsmFI GGGAC 1 cut(s) 831
Bso31I GGTCTC 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 41, 770
Bsp13I TCCGGA 1 cut(s) 613
Bsp143I GATC 5 cut(s) 198, 424, 596, 784, 848
BspACI CCGC 1 cut(s) 31
BspCNI CTCAG 2 cut(s) 126, 758
BspEI TCCGGA 1 cut(s) 613
BspFNI CGCG 1 cut(s) 57
BspHI TCATGA 2 cut(s) 225, 421
BspLI GGNNCC 1 cut(s) 534
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 1 cut(s) 604
BspT104I TTCGAA 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 528
BssHII GCGCGC 1 cut(s) 55
BssMI GATC 5 cut(s) 198, 424, 596, 784, 848
BssNAI GTATAC 1 cut(s) 188
Bst1107I GTATAC 1 cut(s) 188
Bst2UI CCWGG 3 cut(s) 530, 578, 678
Bst4CI ACNGT 1 cut(s) 838
BstBI TTCGAA 1 cut(s) 473
BstC8I GCNNGC 2 cut(s) 57, 151
BstDEI CTNAG 2 cut(s) 134, 745
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 6 cut(s) 109, 316, 355, 586, 598, 727
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 2 cut(s) 57, 59
BstKTI GATC 5 cut(s) 201, 427, 599, 787, 851
BstMAI GTCTC 3 cut(s) 158, 553, 735
BstMBI GATC 5 cut(s) 198, 424, 596, 784, 848
BstNI CCWGG 3 cut(s) 530, 578, 678
BstNSI RCATGY 1 cut(s) 111
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 3 cut(s) 528, 576, 676
BstUI CGCG 1 cut(s) 57
BstV2I GAAGAC 2 cut(s) 104, 401
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
BstZ17I GTATAC 1 cut(s) 188
BtgZI GCGATG 1 cut(s) 168
BtsCI GGATG 6 cut(s) 109, 316, 355, 586, 598, 727
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 465, 834
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 57, 151
CaiI CAGNNNCTG 1 cut(s) 487
CciI TCATGA 2 cut(s) 225, 421
CfoI GCGC 2 cut(s) 57, 59
Cfr13I GGNCC 1 cut(s) 251
CseI GACGC 1 cut(s) 738
Csp6I GTAC 1 cut(s) 131
CviAII CATG 3 cut(s) 108, 226, 422
CviJI RGCY 5 cut(s) 25, 173, 275, 317, 340
CviKI_1 RGCY 5 cut(s) 25, 173, 275, 317, 340
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 2 cut(s) 134, 745
DpnI GATC 5 cut(s) 200, 426, 598, 786, 850
DpnII GATC 5 cut(s) 198, 424, 596, 784, 848
Eco31I GGTCTC 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 98
Eco91I GGTNACC 1 cut(s) 860
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 3 cut(s) 528, 576, 676
EcoT22I ATGCAT 3 cut(s) 6, 109, 673
Esp3I CGTCTC 1 cut(s) 553
FaeI CATG 3 cut(s) 111, 229, 425
FaiI YATR 9 cut(s) 6, 8, 109, 188, 227, 423, 500, 511, 669
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 1 cut(s) 831
FatI CATG 3 cut(s) 107, 225, 421
FauNDI CATATG 1 cut(s) 6
FbaI TGATCA 2 cut(s) 198, 424
FblI GTMKAC 1 cut(s) 187
FokI GGATG 6 cut(s) 116, 323, 362, 593, 605, 734
FspBI CTAG 1 cut(s) 26
GlaI GCGC 2 cut(s) 56, 58
GsuI CTGGAG 1 cut(s) 365
HapII CCGG 1 cut(s) 614
HgaI GACGC 1 cut(s) 738
HhaI GCGC 2 cut(s) 57, 59
Hin1II CATG 3 cut(s) 111, 229, 425
Hin6I GCGC 2 cut(s) 55, 57
HinP1I GCGC 2 cut(s) 55, 57
HinfI GANTC 4 cut(s) 232, 387, 516, 602
HpaII CCGG 1 cut(s) 614
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 3 cut(s) 188, 357, 841
Hpy188I TCNGA 3 cut(s) 117, 601, 711
Hpy188III TCNNGA 9 cut(s) 95, 226, 391, 422, 428, 448, 614, 663, 779
Hpy8I GTNNAC 3 cut(s) 188, 357, 841
HpyAV CCTTC 1 cut(s) 548
HpyCH4III ACNGT 1 cut(s) 838
HpyCH4IV ACGT 1 cut(s) 562
HpyCH4V TGCA 8 cut(s) 4, 20, 107, 142, 502, 671, 725, 830
HpyF3I CTNAG 2 cut(s) 134, 745
HpySE526I ACGT 1 cut(s) 562
Hsp92II CATG 3 cut(s) 111, 229, 425
HspAI GCGC 2 cut(s) 55, 57
Kpn2I TCCGGA 1 cut(s) 613
Ksp22I TGATCA 2 cut(s) 198, 424
Kzo9I GATC 5 cut(s) 198, 424, 596, 784, 848
LmnI GCTCC 1 cut(s) 44
LweI GCATC 2 cut(s) 94, 712
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 562
MaeIII GTNAC 1 cut(s) 860
MalI GATC 5 cut(s) 200, 426, 598, 786, 850
MboI GATC 5 cut(s) 198, 424, 596, 784, 848
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 2 cut(s) 41, 770
MluCI AATT 3 cut(s) 207, 443, 633
MlyI GAGTC 2 cut(s) 396, 525
MmeI TCCRAC 3 cut(s) 81, 288, 361
MnlI CCTC 9 cut(s) 111, 153, 205, 265, 292, 340, 377, 503, 712
Mph1103I ATGCAT 3 cut(s) 6, 109, 673
MroI TCCGGA 1 cut(s) 613
MroXI GAANNNNTTC 2 cut(s) 455, 606
MslI CAYNNNNRTG 1 cut(s) 404
MspI CCGG 1 cut(s) 614
MspR9I CCNGG 3 cut(s) 530, 578, 678
MvaI CCWGG 3 cut(s) 530, 578, 678
MvnI CGCG 1 cut(s) 57
NdeI CATATG 1 cut(s) 6
NdeII GATC 5 cut(s) 198, 424, 596, 784, 848
NlaIII CATG 3 cut(s) 111, 229, 425
NlaIV GGNNCC 1 cut(s) 534
NsiI ATGCAT 3 cut(s) 6, 109, 673
NspI RCATGY 1 cut(s) 111
NspV TTCGAA 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 404
PagI TCATGA 2 cut(s) 225, 421
PaqCI CACCTGC 1 cut(s) 133
PauI GCGCGC 1 cut(s) 55
PdmI GAANNNNTTC 2 cut(s) 455, 606
PfeI GAWTC 2 cut(s) 232, 602
PflMI CCANNNNNTGG 1 cut(s) 683
PfoI TCCNGGA 2 cut(s) 576, 676
PleI GAGTC 2 cut(s) 395, 524
PpsI GAGTC 2 cut(s) 395, 524
Psp6I CCWGG 3 cut(s) 528, 576, 676
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 3 cut(s) 528, 576, 676
PspN4I GGNNCC 1 cut(s) 534
PspPI GGNCC 1 cut(s) 251
PstNI CAGNNNCTG 1 cut(s) 487
PsuI RGATCY 1 cut(s) 784
PteI GCGCGC 1 cut(s) 55
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 404
Sau3AI GATC 5 cut(s) 198, 424, 596, 784, 848
Sau96I GGNCC 1 cut(s) 251
SchI GAGTC 2 cut(s) 396, 525
ScrFI CCNGG 3 cut(s) 530, 578, 678
SduI GDGCHC 2 cut(s) 41, 770
SfaNI GCATC 2 cut(s) 94, 712
SfuI TTCGAA 1 cut(s) 473
SinI GGWCC 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 3 cut(s) 207, 443, 633
SsiI CCGC 1 cut(s) 31
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 3 cut(s) 528, 576, 676
TaaI ACNGT 1 cut(s) 838
TaiI ACGT 1 cut(s) 565
TaqI TCGA 4 cut(s) 176, 270, 473, 514
TasI AATT 3 cut(s) 207, 443, 633
TatI WGTACW 1 cut(s) 130
TfiI GAWTC 2 cut(s) 232, 602
TscAI CASTG 2 cut(s) 472, 841
TspDTI ATGAA 6 cut(s) 242, 260, 303, 344, 350, 728
TspGWI ACGGA 2 cut(s) 243, 858
TspRI CASTG 2 cut(s) 472, 841
Van91I CCANNNNNTGG 1 cut(s) 683
VpaK11BI GGWCC 1 cut(s) 251
XapI RAATTY 2 cut(s) 443, 633
XceI RCATGY 1 cut(s) 111
XmiI GTMKAC 1 cut(s) 187
XmnI GAANNNNTTC 2 cut(s) 455, 606
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 3 cut(s) 6, 109, 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.