Rroxscaffold_2G00152640

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000523
Physical Location & Seq
Reverse (-)
89523918 .. 89526041
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152640.1

Sequence Viewer

Length: 2124 bp
ATGTGCTCTATAAGTACCTCTATGATCAACATCTACGCCATTGGGGATTTACAAATCCGAAAAGTAGATTGGAAAGTGAGAAGATCGATGAGGAATTCAGGTGGGTGGATTCTGGGGACGTTGTTATTTTTGGGTGGATTTTTCTTTGTTCATGTTGCAGCAAGTTCACCAAGAAGTCATCGACTACTGGCGCAAGATGCGGTCGAGATCAAGATGATAAAAAATGGAGTTGTGTTGGATAATAAGCTTGTTCAACTAACATTTTCTAATCCTGGTGGGGATGTTATTGGAATAAGGTATGGAGGAATTGACAACTTACTTGAAATTAACAACAAGGAAGGTAATAGAGGGTACTGGGATGTGGTTTGGAATGACCCGGGAGAGACGGAAAACACACAATACAGACTACAAGGAACACAATTTAAAGTAATCACGGAGAGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAATGCCAACCTCCAGCGGGATGTGACAGTTCCGGTGAATGTTGACAGAAGATTCATAATGCAGCAAGGACGTTCCGGGTTCTATACCTATGCCATCTTCGAGCGCCTTGAAGGGTGGCCGGGAGGGGACATGGACGCAGTTAGAGTGGCTTTCAAGCTTCAGAAAGCCAAGTTTCGATATATGGCGCTATCGGACACTAGGCAAAGGTTCATGCCAACAGCCGATGACCGAGCACGCGGTAAGCCACTTGCATTCAAAGAAGCTGTTCTACTGAACAATGAGACGAGCAGTCCTGAATTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCACCACCGGTGGGATTTTGGATGATCACACCTAGTGCTGAGTTTCGTATGGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTGCCTAACTGGGTTTGTTTCTCATCATTATGTTGGTAAAGAAGTTGGGCTGAAATTCCAAGAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTTTATCTATCTTAACTCGGCAGCACGAAACTCAGCTAATAGTCCTTCAACGATCCGTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCATACAATTTCATTAGTTCTCCCGACATTCCTTCTTCGGATCAACGTGGATCAGTTTCGGGCCAGTTACTAGTACATGATCGATACATTGAAAGTCTTCCAAGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAGGGAAAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTCATTGAAAATATTAGACCCGGTAACTATAATTTGTATGCAACTGTTCCAGGGATAGTTGGAGACTACAAATATGAGGTTGACATTACAATTGAACCAGGAAGTAAAATCCAATTGGCCAGTATTACTTACAAACCTCCGAGAACCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCGGAGTTCTACATACCGGATCCATATCCAACTCTAATGAACAACTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAGTACGGGTTGTGGGCACGTTATTATGAGCGTTACCCTAACAATGACCTCATCTACCGTGTTGGTGCTAACAATTACCGTGAGGATTGGTTTTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGTATGTTGGAACTACATGGCAGATACAGTTTGAACTCAAGAATGTGGTCAATCCCGGAAACTATACACTTCAACTGGCCTTGGCATCAGCCACTTATGCAGAATTGCAAGTTCGGGTTAACAATTTGAATGCGAAGCCACCTCTTTTTTCAACTGGGCTAATAGGCAGCGACAATGCTATAGCAAGAGTTGGAATTCATGGCTTATACTGGTTATGGAGTATTGATGTACCAAGCACTCTACTACGCGAAGGAAGCAACACCATTTATCTTACTCAGTCCAGAGATGGCAATTCACCTTTCCACGGAGTCATGTATGATTATATAAGACTAGAAGGACCTTCTGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

707

Amino Acids

80.23

Weight (kDa)

7.13

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 64 - 263 4.1e-74 Rhamnogalacturonate lyase family
fn3_3 PF14686 426 - 497 7.7e-24 Polysaccharide lyase family 4, domain II
CBM-like PF14683 510 - 700 3.1e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 714, 2016
AciI CCGC 4 cut(s) 200, 493, 714, 1565
AclWI GGATC 6 cut(s) 1099, 1209, 1219, 1318, 1577, 1590
AcoI YGGCCR 5 cut(s) 593, 810, 1160, 1491, 1765
AcsI RAATTY 4 cut(s) 94, 773, 1010, 1962
AcuI CTGAAG 1 cut(s) 620
AdeI CACNNNGTG 1 cut(s) 896
AfaI GTAC 8 cut(s) 16, 353, 806, 1111, 1236, 1613, 1646, 1998
AfiI CCNNNNNNNGG 4 cut(s) 493, 872, 1520, 2072
AgeI ACCGGT 2 cut(s) 868, 1520
AhdI GACNNNNNGTC 1 cut(s) 765
AhlI ACTAGT 1 cut(s) 1231
AjnI CCWGG 3 cut(s) 271, 1423, 1471
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 5 cut(s) 247, 634, 740, 1088, 1562
AluI AGCT 5 cut(s) 247, 634, 740, 1088, 1562
Alw21I GWGCWC 2 cut(s) 8, 712
Alw26I GTCTC 3 cut(s) 377, 752, 1431
AlwI GGATC 6 cut(s) 1099, 1209, 1219, 1318, 1577, 1590
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 8 cut(s) 440, 593, 810, 1160, 1222, 1491, 1765, 1845
ApeKI GCWGC 6 cut(s) 158, 538, 1073, 1292, 1562, 1935
ApoI RAATTY 4 cut(s) 94, 773, 1010, 1962
AsiGI ACCGGT 2 cut(s) 868, 1520
Asp700I GAANNNNTTC 2 cut(s) 741, 1257
AspLEI GCGC 3 cut(s) 193, 582, 664
AspS9I GGNCC 7 cut(s) 917, 931, 950, 1046, 1222, 1523, 2105
AsuC2I CCSGG 7 cut(s) 377, 378, 553, 597, 1299, 1395, 1824
AsuHPI GGTGA 7 cut(s) 159, 460, 523, 855, 928, 1034, 2055
AvaI CYCGRG 1 cut(s) 376
AvaII GGWCC 6 cut(s) 917, 931, 950, 1046, 1523, 2105
BaeGI GKGCMC 1 cut(s) 1660
BalI TGGCCA 2 cut(s) 1162, 1493
BamHI GGATCC 1 cut(s) 1582
BarI GAAGNNNNNNTAC 2 cut(s) 729, 761
BbsI GAAGAC 1 cut(s) 1250
Bbv12I GWGCWC 2 cut(s) 8, 712
BbvI GCAGC 6 cut(s) 170, 550, 1085, 1279, 1549, 1947
BccI CCATC 2 cut(s) 578, 2048
BciT130I CCWGG 3 cut(s) 273, 1425, 1473
BciVI GTATCC 1 cut(s) 1328
BclI TGATCA 2 cut(s) 24, 885
BcnI CCSGG 7 cut(s) 377, 378, 553, 597, 1299, 1395, 1824
BcoDI GTCTC 3 cut(s) 377, 752, 1431
BcuI ACTAGT 1 cut(s) 1231
BfaI CTAG 5 cut(s) 675, 894, 1232, 1749, 2099
BfmI CTRYAG 1 cut(s) 1947
BfoI RGCGCY 2 cut(s) 583, 665
BfuI GTATCC 1 cut(s) 1328
BisI GCNGC 6 cut(s) 159, 539, 1074, 1293, 1563, 1936
BlsI GCNGC 6 cut(s) 160, 540, 1075, 1294, 1564, 1937
BmcAI AGTACT 1 cut(s) 806
Bme18I GGWCC 6 cut(s) 917, 931, 950, 1046, 1523, 2105
BmeRI GACNNNNNGTC 1 cut(s) 765
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 7 cut(s) 917, 931, 950, 1046, 1222, 1523, 2105
BmiI GGNNCC 1 cut(s) 1584
BmrI ACTGGG 3 cut(s) 364, 975, 1932
BmsI GCATC 2 cut(s) 187, 1862
BmuI ACTGGG 3 cut(s) 364, 975, 1932
BpiI GAAGAC 1 cut(s) 1250
BpmI CTGGAG 1 cut(s) 473
BpuEI CTTGAG 2 cut(s) 1725, 1790
BpuMI CCSGG 7 cut(s) 377, 378, 553, 597, 1299, 1395, 1824
Bsa29I ATCGAT 2 cut(s) 86, 1243
BsaAI YACGTR 1 cut(s) 1282
BsaBI GATNNNNATC 3 cut(s) 29, 798, 1587
BsaJI CCNNGG 6 cut(s) 376, 813, 1262, 1424, 1848, 2071
BsaWI WCCGGW 4 cut(s) 508, 868, 1520, 1579
Bsc4I CCNNNNNNNGG 4 cut(s) 493, 872, 1520, 2072
Bse118I RCCGGY 3 cut(s) 868, 1520, 1767
Bse1I ACTGG 8 cut(s) 192, 359, 970, 1225, 1494, 1848, 1927, 1982
Bse8I GATNNNNATC 3 cut(s) 29, 798, 1587
BseBI CCWGG 3 cut(s) 273, 1425, 1473
BseCI ATCGAT 2 cut(s) 86, 1243
BseDI CCNNGG 6 cut(s) 376, 813, 1262, 1424, 1848, 2071
BseGI GGATG 5 cut(s) 286, 364, 502, 796, 888
BseJI GATNNNNATC 3 cut(s) 29, 798, 1587
BseLI CCNNNNNNNGG 4 cut(s) 493, 872, 1520, 2072
BseMII CTCAG 4 cut(s) 891, 1098, 2057, 2103
BseNI ACTGG 8 cut(s) 192, 359, 970, 1225, 1494, 1848, 1927, 1982
BseSI GKGCMC 1 cut(s) 1660
BseX3I CGGCCG 1 cut(s) 810
BseXI GCAGC 6 cut(s) 170, 550, 1085, 1279, 1549, 1947
BsgI GTGCAG 1 cut(s) 1278
Bsh1236I CGCG 2 cut(s) 714, 2016
Bsh1285I CGRYCG 2 cut(s) 204, 813
BshFI GGCC 8 cut(s) 442, 595, 812, 1162, 1224, 1493, 1767, 1847
BshTI ACCGGT 2 cut(s) 868, 1520
BshVI ATCGAT 2 cut(s) 86, 1243
BsiEI CGRYCG 2 cut(s) 204, 813
BsiHKAI GWGCWC 2 cut(s) 8, 712
BsiHKCI CYCGRG 1 cut(s) 376
BslFI GGGAC 2 cut(s) 130, 617
BslI CCNNNNNNNGG 4 cut(s) 493, 872, 1520, 2072
BsmAI GTCTC 3 cut(s) 377, 752, 1431
BsmBI CGTCTC 2 cut(s) 377, 752
BsmFI GGGAC 2 cut(s) 130, 617
BsmI GAATGC 3 cut(s) 728, 1545, 1903
BsnI GGCC 8 cut(s) 442, 595, 812, 1162, 1224, 1493, 1767, 1847
BsoBI CYCGRG 1 cut(s) 376
Bsp1286I GDGCHC 3 cut(s) 8, 712, 1660
Bsp1407I TGTACA 1 cut(s) 1611
BspACI CCGC 4 cut(s) 200, 493, 714, 1565
BspANI GGCC 8 cut(s) 442, 595, 812, 1162, 1224, 1493, 1767, 1847
BspCNI CTCAG 4 cut(s) 892, 1097, 2056, 2104
BspDI ATCGAT 2 cut(s) 86, 1243
BspFNI CGCG 2 cut(s) 714, 2016
BspLI GGNNCC 1 cut(s) 1584
BspPI GGATC 6 cut(s) 1099, 1209, 1219, 1318, 1577, 1590
BsrFI RCCGGY 3 cut(s) 868, 1520, 1767
BsrGI TGTACA 1 cut(s) 1611
BsrI ACTGG 8 cut(s) 192, 359, 970, 1225, 1494, 1848, 1927, 1982
BssAI RCCGGY 3 cut(s) 868, 1520, 1767
BssECI CCNNGG 6 cut(s) 376, 813, 1262, 1424, 1848, 2071
BssT1I CCWWGG 2 cut(s) 1262, 1848
Bst2UI CCWGG 3 cut(s) 273, 1425, 1473
Bst4CI ACNGT 5 cut(s) 505, 1420, 1700, 1721, 1797
BstAUI TGTACA 1 cut(s) 1611
BstBAI YACGTR 1 cut(s) 1282
BstC8I GCNNGC 1 cut(s) 712
BstDEI CTNAG 4 cut(s) 900, 1084, 2043, 2112
BstDSI CCRYGG 1 cut(s) 2071
BstF5I GGATG 5 cut(s) 286, 364, 502, 796, 888
BstFNI CGCG 2 cut(s) 714, 2016
BstH2I RGCGCY 2 cut(s) 583, 665
BstHHI GCGC 3 cut(s) 193, 582, 664
BstMAI GTCTC 3 cut(s) 377, 752, 1431
BstMCI CGRYCG 2 cut(s) 204, 813
BstMWI GCNNNNNNNGC 3 cut(s) 197, 1865, 2022
BstNI CCWGG 3 cut(s) 273, 1425, 1473
BstSFI CTRYAG 1 cut(s) 1947
BstSLI GKGCMC 1 cut(s) 1660
BstSNI TACGTA 1 cut(s) 1282
BstUI CGCG 2 cut(s) 714, 2016
BstV1I GCAGC 6 cut(s) 170, 550, 1085, 1279, 1549, 1947
BstV2I GAAGAC 1 cut(s) 1250
BstX2I RGATCY 1 cut(s) 1582
BstYI RGATCY 1 cut(s) 1582
BstZI CGGCCG 1 cut(s) 810
Bsu15I ATCGAT 2 cut(s) 86, 1243
BsuI GTATCC 1 cut(s) 1328
BsuRI GGCC 8 cut(s) 442, 595, 812, 1162, 1224, 1493, 1767, 1847
BsuTUI ATCGAT 2 cut(s) 86, 1243
BtgI CCRYGG 1 cut(s) 2071
BtsCI GGATG 5 cut(s) 286, 364, 502, 796, 888
Cac8I GCNNGC 1 cut(s) 712
CfoI GCGC 3 cut(s) 193, 582, 664
Cfr10I RCCGGY 3 cut(s) 868, 1520, 1767
Cfr13I GGNCC 7 cut(s) 917, 931, 950, 1046, 1222, 1523, 2105
Cfr9I CCCGGG 1 cut(s) 376
ClaI ATCGAT 2 cut(s) 86, 1243
CseI GACGC 1 cut(s) 620
Csp6I GTAC 8 cut(s) 15, 352, 805, 1110, 1235, 1612, 1645, 1997
CspAI ACCGGT 2 cut(s) 868, 1520
CviAII CATG 9 cut(s) 152, 607, 688, 836, 1238, 1314, 1785, 1967, 2080
CviQI GTAC 8 cut(s) 15, 352, 805, 1110, 1235, 1612, 1645, 1997
DdeI CTNAG 4 cut(s) 900, 1084, 2043, 2112
DraI TTTAAA 1 cut(s) 424
DraIII CACNNNGTG 1 cut(s) 896
DriI GACNNNNNGTC 1 cut(s) 765
EaeI YGGCCR 5 cut(s) 593, 810, 1160, 1491, 1765
EagI CGGCCG 1 cut(s) 810
Eam1105I GACNNNNNGTC 1 cut(s) 765
EclXI CGGCCG 1 cut(s) 810
Eco105I TACGTA 1 cut(s) 1282
Eco130I CCWWGG 2 cut(s) 1262, 1848
Eco32I GATATC 1 cut(s) 826
Eco47I GGWCC 6 cut(s) 917, 931, 950, 1046, 1523, 2105
Eco52I CGGCCG 1 cut(s) 810
Eco57I CTGAAG 1 cut(s) 620
Eco88I CYCGRG 1 cut(s) 376
EcoO109I RGGNCCY 2 cut(s) 931, 2105
EcoRI GAATTC 2 cut(s) 94, 1962
EcoRII CCWGG 3 cut(s) 271, 1423, 1471
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 2 cut(s) 1262, 1848
ErhI CCWWGG 2 cut(s) 1262, 1848
Esp3I CGTCTC 2 cut(s) 377, 752
FaeI CATG 9 cut(s) 155, 610, 691, 839, 1241, 1317, 1788, 1970, 2083
FalI AAGNNNNNCTT 2 cut(s) 1261, 1293
FaqI GGGAC 2 cut(s) 130, 617
FatI CATG 9 cut(s) 151, 606, 687, 835, 1237, 1313, 1784, 1966, 2079
FauI CCCGC 1 cut(s) 486
FbaI TGATCA 2 cut(s) 24, 885
Fnu4HI GCNGC 6 cut(s) 159, 539, 1074, 1293, 1563, 1936
FokI GGATG 5 cut(s) 293, 371, 509, 803, 895
Fsp4HI GCNGC 6 cut(s) 159, 539, 1074, 1293, 1563, 1936
FspBI CTAG 5 cut(s) 675, 894, 1232, 1749, 2099
GlaI GCGC 3 cut(s) 192, 581, 663
GluI GCNGC 6 cut(s) 159, 539, 1074, 1293, 1563, 1936
GsuI CTGGAG 1 cut(s) 473
HaeII RGCGCY 2 cut(s) 583, 665
HaeIII GGCC 8 cut(s) 442, 595, 812, 1162, 1224, 1493, 1767, 1847
HgaI GACGC 1 cut(s) 620
HhaI GCGC 3 cut(s) 193, 582, 664
Hin1II CATG 9 cut(s) 155, 610, 691, 839, 1241, 1317, 1788, 1970, 2083
Hin6I GCGC 3 cut(s) 191, 580, 662
HinP1I GCGC 3 cut(s) 191, 580, 662
HincII GTYRAC 3 cut(s) 520, 1456, 1888
HindII GTYRAC 3 cut(s) 520, 1456, 1888
HindIII AAGCTT 2 cut(s) 245, 632
HinfI GANTC 5 cut(s) 109, 528, 1348, 1621, 2076
HpaI GTTAAC 1 cut(s) 1888
HphI GGTGA 7 cut(s) 159, 460, 523, 855, 928, 1034, 2055
Hpy166II GTNNAC 5 cut(s) 167, 520, 1112, 1456, 1888
Hpy188I TCNGA 7 cut(s) 59, 639, 670, 1046, 1201, 1515, 2113
Hpy188III TCNNGA 7 cut(s) 205, 211, 770, 1184, 1550, 1807, 2049
Hpy8I GTNNAC 5 cut(s) 167, 520, 1112, 1456, 1888
HpyAV CCTTC 8 cut(s) 332, 581, 773, 1107, 1203, 2012, 2096, 2118
HpyCH4III ACNGT 5 cut(s) 505, 1420, 1700, 1721, 1797
HpyCH4IV ACGT 6 cut(s) 119, 547, 945, 1207, 1281, 1660
HpyCH4V TGCA 8 cut(s) 158, 538, 728, 835, 1295, 1415, 1868, 1876
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 1865, 2022
HpyF3I CTNAG 4 cut(s) 900, 1084, 2043, 2112
HpySE526I ACGT 6 cut(s) 119, 547, 945, 1207, 1281, 1660
Hsp92II CATG 9 cut(s) 155, 610, 691, 839, 1241, 1317, 1788, 1970, 2083
HspAI GCGC 3 cut(s) 191, 580, 662
Ksp22I TGATCA 2 cut(s) 24, 885
KspAI GTTAAC 1 cut(s) 1888
Lsp1109I GCAGC 6 cut(s) 170, 550, 1085, 1279, 1549, 1947
LweI GCATC 2 cut(s) 187, 1862
MaeI CTAG 5 cut(s) 675, 894, 1232, 1749, 2099
MaeII ACGT 6 cut(s) 119, 547, 945, 1207, 1281, 1660
MaeIII GTNAC 5 cut(s) 499, 1227, 1397, 1673, 1744
MboII GAAGA 6 cut(s) 93, 537, 565, 1157, 1188, 1250
MfeI CAATTG 2 cut(s) 1464, 1487
MflI RGATCY 1 cut(s) 1582
MhlI GDGCHC 3 cut(s) 8, 712, 1660
MlsI TGGCCA 2 cut(s) 1162, 1493
MluNI TGGCCA 2 cut(s) 1162, 1493
MlyI GAGTC 3 cut(s) 1342, 1630, 2085
MmeI TCCRAC 9 cut(s) 216, 821, 928, 1288, 1414, 1608, 1616, 1756, 1939
Mox20I TGGCCA 2 cut(s) 1162, 1493
MroXI GAANNNNTTC 2 cut(s) 741, 1257
MscI TGGCCA 2 cut(s) 1162, 1493
MseI TTAA 4 cut(s) 327, 423, 1065, 1887
MslI CAYNNNNRTG 1 cut(s) 984
Msp20I TGGCCA 2 cut(s) 1162, 1493
MspA1I CMGCKG 2 cut(s) 493, 1562
MunI CAATTG 2 cut(s) 1464, 1487
Mva1269I GAATGC 3 cut(s) 728, 1545, 1903
MvaI CCWGG 3 cut(s) 273, 1425, 1473
MvnI CGCG 2 cut(s) 714, 2016
MwoI GCNNNNNNNGC 3 cut(s) 197, 1865, 2022
NciI CCSGG 7 cut(s) 377, 378, 553, 597, 1299, 1395, 1824
NlaIII CATG 9 cut(s) 155, 610, 691, 839, 1241, 1317, 1788, 1970, 2083
NlaIV GGNNCC 1 cut(s) 1584
NmeAIII GCCGAG 3 cut(s) 788, 838, 1049
NmuCI GTSAC 2 cut(s) 499, 1744
PctI GAATGC 3 cut(s) 728, 1545, 1903
PdmI GAANNNNTTC 2 cut(s) 741, 1257
PfeI GAWTC 2 cut(s) 109, 528
PfoI TCCNGGA 1 cut(s) 1822
PinAI ACCGGT 2 cut(s) 868, 1520
PkrI GCNGC 6 cut(s) 160, 540, 1075, 1294, 1564, 1937
PleI GAGTC 3 cut(s) 1342, 1629, 2084
PpsI GAGTC 3 cut(s) 1342, 1629, 2084
Ppu21I YACGTR 1 cut(s) 1282
PpuMI RGGWCCY 2 cut(s) 931, 2105
Psp5II RGGWCCY 2 cut(s) 931, 2105
Psp6I CCWGG 3 cut(s) 271, 1423, 1471
PspGI CCWGG 3 cut(s) 271, 1423, 1471
PspN4I GGNNCC 1 cut(s) 1584
PspPI GGNCC 7 cut(s) 917, 931, 950, 1046, 1222, 1523, 2105
PspPPI RGGWCCY 2 cut(s) 931, 2105
PsrI GAACNNNNNNTAC 4 cut(s) 1510, 1542, 1595, 1627
PsuI RGATCY 1 cut(s) 1582
PvuII CAGCTG 1 cut(s) 1562
RsaI GTAC 8 cut(s) 16, 353, 806, 1111, 1236, 1613, 1646, 1998
RsaNI GTAC 8 cut(s) 15, 352, 805, 1110, 1235, 1612, 1645, 1997
RseI CAYNNNNRTG 1 cut(s) 984
SaqAI TTAA 4 cut(s) 327, 423, 1065, 1887
SatI GCNGC 6 cut(s) 159, 539, 1074, 1293, 1563, 1936
Sau96I GGNCC 7 cut(s) 917, 931, 950, 1046, 1222, 1523, 2105
ScaI AGTACT 1 cut(s) 806
SchI GAGTC 3 cut(s) 1342, 1630, 2085
SduI GDGCHC 3 cut(s) 8, 712, 1660
SfaNI GCATC 2 cut(s) 187, 1862
SfcI CTRYAG 1 cut(s) 1947
SgrAI CRCCGGYG 1 cut(s) 868
SinI GGWCC 6 cut(s) 917, 931, 950, 1046, 1523, 2105
SmaI CCCGGG 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 984
SmlI CTYRAG 2 cut(s) 1740, 1805
SmoI CTYRAG 2 cut(s) 1740, 1805
SnaBI TACGTA 1 cut(s) 1282
SpeI ACTAGT 1 cut(s) 1231
SsiI CCGC 4 cut(s) 200, 493, 714, 1565
SspI AATATT 1 cut(s) 1387
SspMI CTAG 5 cut(s) 675, 894, 1232, 1749, 2099
StyI CCWWGG 2 cut(s) 1262, 1848
TaaI ACNGT 5 cut(s) 505, 1420, 1700, 1721, 1797
TaiI ACGT 6 cut(s) 122, 550, 948, 1210, 1284, 1663
TaqI TCGA 6 cut(s) 86, 181, 204, 576, 652, 1243
TaqII GACCGA 1 cut(s) 720
TatI WGTACW 3 cut(s) 804, 1234, 1611
TfiI GAWTC 2 cut(s) 109, 528
Tru1I TTAA 4 cut(s) 327, 423, 1065, 1887
Tru9I TTAA 4 cut(s) 327, 423, 1065, 1887
TseFI GTSAC 2 cut(s) 499, 1744
TseI GCWGC 6 cut(s) 158, 538, 1073, 1292, 1562, 1935
Tsp45I GTSAC 2 cut(s) 499, 1744
TspDTI ATGAA 8 cut(s) 140, 454, 520, 676, 1162, 1374, 1616, 1955
TspGWI ACGGA 4 cut(s) 401, 449, 1097, 2088
TspMI CCCGGG 1 cut(s) 376
VpaK11BI GGWCC 6 cut(s) 917, 931, 950, 1046, 1523, 2105
XapI RAATTY 4 cut(s) 94, 773, 1010, 1962
XmaI CCCGGG 1 cut(s) 376
XmnI GAANNNNTTC 2 cut(s) 741, 1257
XspI CTAG 5 cut(s) 675, 894, 1232, 1749, 2099
ZrmI AGTACT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.