Rh2BG228800

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000523
Physical Location & Seq
Forward (+)
22404080 .. 22413989
9910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG228800.1

Sequence Viewer

Length: 2358 bp
ATGGAGAAGATGAGGAAATGGGGTTGGCTTCTGGGGTCATTAGCAGCAATGCTTCTTCTGGTTTTGTTCTTGGCTGGTGATGGAACTTACGACGAGACGACGAGCCGAGTAAGACGATCACTGAGGAGCATAATTCCTCGGCGACAACAGGGGGTCAATTTACTAACAAGGGACCCCAAGAGGGTTGTTTTGGATAATGGCATTGTCAAAGTCATTTTCTCTAATCCGGGCGGATACGTTATTGGTATACAGTACAGAGGCCTTGACATACTTGAAATGGGGAATGAAGAAAATAATAGAGGGTACTGGGATGTGGTCTGGAAAAAGAATATAACTGACAAACTAGAAGCAACAAAATTTAGGGTTATTACCGCGAGGTCAGACCAAGCAGAAATTTCCTTCCTAAGAACATATGATGTTTCCCTTGGTAACGGCACGAACATAATTCCCTTGACCGTAGACAAAAGGTACATACTGCAGCGGGGTGGTTCTGGTTTCTATGCATATGGAATATTCGAGCGCCTTGATGGGTGGCCGGCGGTACAGGATATGGATCAAATTAGAATGGTTTACAAGCTTGAACAAGCCAAATTTCATTATATGGCGTTATCAGACACCAGGCAAAGAAGCATGCCAACAGCAGCAGACCGTGGTCGTGGTCAGAAACTTGCGTATAAAGAAGCTTGTCTTTTGACTAATCCAAGCAATCCTGACTTTAGAGGAGAGGTAGATGACAAGTACCAGTACTCAATCGAGGACAAAGATAACAAAATTCATGGTTGGATTAGTACTGATGAATCTGTGGGATTCTGGATGATCACACCCAGCGACGAATTTCGTACAGGAGGCCCCTTTAAGCAGGACCTCACCTCCCATGTTGGTCCAACCACCCTCTCCATGTTTGTTAGTACTCATTATGCTGGGAAGGAACTTCAGATCAAATTTGAAGAAGGTGAGGCATGGAAAAAGGTTTTTGGGCCTGTCTTGGTGTATCTTAATTCGGCTCCAAGCTCAAAGAATTCCATCCGTATACTTTGGAATGATGCTAAAAAGCAGATGCTTAAAGAAGTCAAGAACTGGCCGTATAATTTCACTCAATCCGAGGACTTTCCTTCTTCCCATCAACGGGGATCAGTTTTGGGCCAATTACTAATACATGATCGATACATCAATGAGAGTTTTTTCCCAGCAAGTTCTGCTTATGTGGGATTGGCTGCACCCGGAGAAGTGGGATCATGGCAAAGGGAAAACAAGGGTTATCAATTTTGGAATCAAGCTGACGAAGAAGGTAGATTCATTATCAAAAATGTTCGACCCGGAAACTACAGTTTTTATGCATGGGTTCCTGGCATTGTTGGGGATTACAAGTATGAGGTTGATATTACAATTACTCCAGGGTCTAATATCAAATTGGACAATCTTACTTACGAACCTCCAAGAAATGGTCCTACTTTGTGGGAAATTGGCATCCCTGATCGTACTGCTGCCGAATTCTACGTACCAGATCCTTATCCAACTCTTTTGAACAATTTATACACCCAGAATCACACAGAAAATTTTAGGCAATACGGGTTGTGGGCACGTTATGGAGAGCGACACCCTCATAATGATCTTGTGTACACCGTAGATATCAACAATTACCATGATGATTGGTTCTATGCTCAAGTGACCAGAAATACTGGAAATGAAACCTATGTAGGAACGACGTGGCAAATTCTATTTGAACTTCATAATGTCACAAATCCAGGAAATTATACACTCCAATTGGCATTGGCCTCAGCAAACAATGCAGAATTGCAGGTTCGAATTAACAACAAGAGCGATGAGCAACCTCATTTTTCATCAAAGTTAATTGGAGGGGACAATGCGATAGCAAGACATGGAATTCATGGTTTGTACTGGTTGTTTAGTGTTGAGATACCAAGCTCGCTACTAAAAGAAGGAATCAACACCGTCTATCTTACCCAAGCGAGAGGTGGTGAAACTTTCCAAGGAGTCATGTATGACTACATCAGACTAGAAGCACCTTCATCACACACTGATTCACAAGTGAAGGAAAATAATCTCAGTGGTCAACCAAAACAGTCACATTATTTGGGTTCGCATTATCACAGAGGTAAAGCTGGAGGTCCAGACTTGAAAAGTTTCATTGTCCAATTTCCTGTGTCCCGAGAAGGTCCAGACTTGAAAAGTTTCATTGTCCAATTTCCTGTGTCCAGAGAAGGAAGTTTAGTGCAGGAAAGCAGGGGTAGGTACACGAAACCAACCATAGAGTTGGAGGAAAACAATCACAGAGGATTTCTTGGTGGCTCACACAGTGGCAATAATATGGAGCTTTCAAAAATATGGAGCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

785

Amino Acids

89.47

Weight (kDa)

6.72

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 52 - 243 1.2e-73 Rhamnogalacturonate lyase family
fn3_3 PF14686 398 - 471 6e-25 Polysaccharide lyase family 4, domain II
CBM-like PF14683 484 - 673 6.3e-50 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1789
AccB7I CCANNNNNTGG 1 cut(s) 1442
AccI GTMKAC 3 cut(s) 247, 459, 1030
AccII CGCG 1 cut(s) 374
AciI CCGC 4 cut(s) 231, 372, 481, 539
AclWI GGATC 4 cut(s) 561, 1138, 1240, 1499
AcoI YGGCCR 2 cut(s) 533, 1079
AcuI CTGAAG 1 cut(s) 917
AdeI CACNNNGTG 1 cut(s) 2318
AfiI CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
AgsI TTSAA 8 cut(s) 275, 581, 947, 1525, 1724, 2140, 2188, 2340
AjiI CACGTC 1 cut(s) 1707
AjnI CCWGG 4 cut(s) 617, 1345, 1393, 1744
AluBI AGCT 7 cut(s) 577, 683, 1011, 1277, 1926, 2123, 2335
AluI AGCT 7 cut(s) 577, 683, 1011, 1277, 1926, 2123, 2335
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 4 cut(s) 561, 1138, 1240, 1499
Ama87I CYCGRG 1 cut(s) 2169
AoxI GGCC 7 cut(s) 259, 533, 847, 977, 1079, 1141, 1773
ApeKI GCWGC 5 cut(s) 44, 478, 641, 1214, 1484
Asp700I GAANNNNTTC 2 cut(s) 2144, 2192
AspLEI GCGC 1 cut(s) 522
AspS9I GGNCC 9 cut(s) 172, 848, 862, 881, 977, 1141, 1445, 2129, 2177
AsuC2I CCSGG 3 cut(s) 228, 1221, 1317
AsuHPI GGTGA 4 cut(s) 89, 859, 965, 1991
AsuII TTCGAA 1 cut(s) 1804
AvaI CYCGRG 1 cut(s) 2169
AvaII GGWCC 6 cut(s) 172, 862, 881, 1445, 2129, 2177
BaeGI GKGCMC 1 cut(s) 1582
BbvCI CCTCAGC 1 cut(s) 1777
BbvI GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
BccI CCATC 4 cut(s) 74, 521, 1031, 1128
BceAI ACGGC 2 cut(s) 448, 1066
BciT130I CCWGG 4 cut(s) 619, 1347, 1395, 1746
BciVI GTATCC 1 cut(s) 227
BclI TGATCA 1 cut(s) 816
BcnI CCSGG 3 cut(s) 228, 1221, 1317
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 2 cut(s) 344, 2018
BfmI CTRYAG 2 cut(s) 476, 1324
BfoI RGCGCY 1 cut(s) 523
BfuAI ACCTGC 1 cut(s) 1789
BfuI GTATCC 1 cut(s) 227
BisI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
BlsI GCNGC 5 cut(s) 46, 480, 643, 1216, 1486
BmcAI AGTACT 3 cut(s) 746, 790, 910
Bme1390I CCNGG 7 cut(s) 228, 619, 1221, 1317, 1347, 1395, 1746
Bme18I GGWCC 6 cut(s) 172, 862, 881, 1445, 2129, 2177
BmeT110I CYCGRG 1 cut(s) 2169
BmgBI CACGTC 1 cut(s) 1707
BmgT120I GGNCC 9 cut(s) 172, 848, 862, 881, 977, 1141, 1445, 2129, 2177
BmiI GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
BmrFI CCNGG 7 cut(s) 228, 619, 1221, 1317, 1347, 1395, 1746
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 3 cut(s) 1033, 1047, 1476
BmuI ACTGGG 1 cut(s) 316
BoxI GACNNNNGTC 1 cut(s) 651
BpmI CTGGAG 2 cut(s) 1377, 2145
Bpu10I CCTNAGC 1 cut(s) 1777
Bpu14I TTCGAA 1 cut(s) 1804
BpuEI CTTGAG 1 cut(s) 1647
BpuMI CCSGG 3 cut(s) 228, 1221, 1317
Bsa29I ATCGAT 1 cut(s) 1162
BsaAI YACGTR 1 cut(s) 1498
BsaBI GATNNNNATC 2 cut(s) 552, 1509
BsaJI CCNNGG 6 cut(s) 137, 424, 649, 1101, 1394, 1990
BsaXI ACNNNNNCTCC 4 cut(s) 854, 884, 1375, 1405
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
Bse118I RCCGGY 1 cut(s) 535
Bse1I ACTGG 5 cut(s) 311, 742, 1082, 1684, 1904
Bse3DI GCAATG 1 cut(s) 54
Bse8I GATNNNNATC 2 cut(s) 552, 1509
BseBI CCWGG 4 cut(s) 619, 1347, 1395, 1746
BseCI ATCGAT 1 cut(s) 1162
BseDI CCNNGG 6 cut(s) 137, 424, 649, 1101, 1394, 1990
BseGI GGATG 4 cut(s) 316, 819, 1023, 1467
BseJI GATNNNNATC 2 cut(s) 552, 1509
BseLI CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 3 cut(s) 113, 1791, 2080
BseNI ACTGG 5 cut(s) 311, 742, 1082, 1684, 1904
BseRI GAGGAG 2 cut(s) 139, 735
BseSI GKGCMC 1 cut(s) 1582
BseXI GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
BseYI CCCAGC 3 cut(s) 824, 920, 1186
BsgI GTGCAG 2 cut(s) 1200, 2255
Bsh1236I CGCG 1 cut(s) 374
BshFI GGCC 7 cut(s) 261, 535, 849, 979, 1081, 1143, 1775
BshVI ATCGAT 1 cut(s) 1162
BsiHKCI CYCGRG 1 cut(s) 2169
BsiSI CCGG 4 cut(s) 227, 536, 1221, 1317
BslFI GGGAC 3 cut(s) 185, 1874, 2152
BslI CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
BsmAI GTCTC 1 cut(s) 89
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 3 cut(s) 185, 1874, 2152
BsnI GGCC 7 cut(s) 261, 535, 849, 979, 1081, 1143, 1775
BsoBI CYCGRG 1 cut(s) 2169
Bsp119I TTCGAA 1 cut(s) 1804
Bsp1286I GDGCHC 1 cut(s) 1582
Bsp1407I TGTACA 1 cut(s) 1617
BspACI CCGC 4 cut(s) 231, 372, 481, 539
BspANI GGCC 7 cut(s) 261, 535, 849, 979, 1081, 1143, 1775
BspCNI CTCAG 3 cut(s) 114, 1790, 2079
BspDI ATCGAT 1 cut(s) 1162
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
BspMAI CTGCAG 1 cut(s) 480
BspMI ACCTGC 1 cut(s) 1789
BspPI GGATC 4 cut(s) 561, 1138, 1240, 1499
BspT104I TTCGAA 1 cut(s) 1804
BsrDI GCAATG 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 535
BsrGI TGTACA 1 cut(s) 1617
BsrI ACTGG 5 cut(s) 311, 742, 1082, 1684, 1904
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 6 cut(s) 137, 424, 649, 1101, 1394, 1990
BssNAI GTATAC 2 cut(s) 248, 1031
BssT1I CCWWGG 2 cut(s) 424, 1990
Bst1107I GTATAC 2 cut(s) 248, 1031
Bst2UI CCWGG 4 cut(s) 619, 1347, 1395, 1746
Bst4CI ACNGT 8 cut(s) 252, 457, 650, 1328, 1624, 1954, 2085, 2318
BstAPI GCANNNNNTGC 2 cut(s) 1196, 1787
BstAUI TGTACA 1 cut(s) 1617
BstBAI YACGTR 1 cut(s) 1498
BstBI TTCGAA 1 cut(s) 1804
BstC8I GCNNGC 3 cut(s) 537, 632, 1928
BstDEI CTNAG 4 cut(s) 122, 404, 1777, 2066
BstDSI CCRYGG 1 cut(s) 649
BstF5I GGATG 4 cut(s) 316, 819, 1023, 1467
BstFNI CGCG 1 cut(s) 374
BstH2I RGCGCY 1 cut(s) 523
BstHHI GCGC 1 cut(s) 522
BstMAI GTCTC 1 cut(s) 89
BstMWI GCNNNNNNNGC 2 cut(s) 1196, 1787
BstNI CCWGG 4 cut(s) 619, 1347, 1395, 1746
BstNSI RCATGY 1 cut(s) 634
BstPAI GACNNNNGTC 1 cut(s) 651
BstSCI CCNGG 7 cut(s) 226, 617, 1219, 1315, 1345, 1393, 1744
BstSFI CTRYAG 2 cut(s) 476, 1324
BstSLI GKGCMC 1 cut(s) 1582
BstSNI TACGTA 1 cut(s) 1498
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
BstX2I RGATCY 1 cut(s) 1504
BstXI CCANNNNNNTGG 1 cut(s) 2275
BstYI RGATCY 1 cut(s) 1504
BstZ17I GTATAC 2 cut(s) 248, 1031
Bsu15I ATCGAT 1 cut(s) 1162
BsuI GTATCC 1 cut(s) 227
BsuRI GGCC 7 cut(s) 261, 535, 849, 979, 1081, 1143, 1775
BsuTUI ATCGAT 1 cut(s) 1162
BtgI CCRYGG 1 cut(s) 649
BtgZI GCGATG 1 cut(s) 1836
BtrI CACGTC 1 cut(s) 1707
BtsCI GGATG 4 cut(s) 316, 819, 1023, 1467
BtsIMutI CAGTG 4 cut(s) 119, 2037, 2074, 2323
BveI ACCTGC 1 cut(s) 1789
Cac8I GCNNGC 3 cut(s) 537, 632, 1928
CfoI GCGC 1 cut(s) 522
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 9 cut(s) 172, 848, 862, 881, 977, 1141, 1445, 2129, 2177
ClaI ATCGAT 1 cut(s) 1162
DdeI CTNAG 4 cut(s) 122, 404, 1777, 2066
DraIII CACNNNGTG 1 cut(s) 2318
EaeI YGGCCR 2 cut(s) 533, 1079
EciI GGCGGA 1 cut(s) 246
Eco105I TACGTA 1 cut(s) 1498
Eco130I CCWWGG 2 cut(s) 424, 1990
Eco147I AGGCCT 1 cut(s) 261
Eco32I GATATC 1 cut(s) 1630
Eco47I GGWCC 6 cut(s) 172, 862, 881, 1445, 2129, 2177
Eco57I CTGAAG 1 cut(s) 917
Eco88I CYCGRG 1 cut(s) 2169
EcoO109I RGGNCCY 3 cut(s) 172, 848, 862
EcoRI GAATTC 3 cut(s) 1018, 1490, 1884
EcoRII CCWGG 4 cut(s) 617, 1345, 1393, 1744
EcoRV GATATC 1 cut(s) 1630
EcoT14I CCWWGG 2 cut(s) 424, 1990
EcoT22I ATGCAT 2 cut(s) 505, 1339
ErhI CCWWGG 2 cut(s) 424, 1990
Esp3I CGTCTC 1 cut(s) 89
FalI AAGNNNNNCTT 4 cut(s) 672, 704, 1183, 1215
FaqI GGGAC 3 cut(s) 185, 1874, 2152
FauI CCCGC 1 cut(s) 474
FauNDI CATATG 2 cut(s) 412, 505
FbaI TGATCA 1 cut(s) 816
FblI GTMKAC 3 cut(s) 247, 459, 1030
Fnu4HI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
FokI GGATG 4 cut(s) 323, 826, 1010, 1454
Fsp4HI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
FspBI CTAG 2 cut(s) 344, 2018
GlaI GCGC 1 cut(s) 521
GluI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
GsaI CCCAGC 3 cut(s) 828, 924, 1190
GsuI CTGGAG 2 cut(s) 1377, 2145
HaeII RGCGCY 1 cut(s) 523
HaeIII GGCC 7 cut(s) 261, 535, 849, 979, 1081, 1143, 1775
HapII CCGG 4 cut(s) 227, 536, 1221, 1317
HhaI GCGC 1 cut(s) 522
Hin6I GCGC 1 cut(s) 520
HinP1I GCGC 1 cut(s) 520
HincII GTYRAC 1 cut(s) 2075
HindII GTYRAC 1 cut(s) 2075
HindIII AAGCTT 2 cut(s) 575, 681
HinfI GANTC 8 cut(s) 797, 807, 1270, 1293, 1543, 1944, 1995, 2042
HpaII CCGG 4 cut(s) 227, 536, 1221, 1317
HphI GGTGA 4 cut(s) 89, 859, 965, 1991
Hpy166II GTNNAC 8 cut(s) 248, 460, 571, 1031, 1618, 1620, 2075, 2256
Hpy188I TCNGA 6 cut(s) 382, 613, 663, 936, 1102, 2015
Hpy188III TCNNGA 8 cut(s) 319, 710, 811, 1072, 2132, 2169, 2180, 2217
Hpy8I GTNNAC 8 cut(s) 248, 460, 571, 1031, 1618, 1620, 2075, 2256
Hpy99I CGWCG 4 cut(s) 95, 103, 833, 1708
HpyCH4III ACNGT 8 cut(s) 252, 457, 650, 1328, 1624, 1954, 2085, 2318
HpyCH4IV ACGT 4 cut(s) 237, 1497, 1582, 1706
HpyCH4V TGCA 7 cut(s) 478, 503, 1217, 1337, 1790, 1798, 2236
HpyF10VI GCNNNNNNNGC 2 cut(s) 1196, 1787
HpyF3I CTNAG 4 cut(s) 122, 404, 1777, 2066
HpySE526I ACGT 4 cut(s) 237, 1497, 1582, 1706
HspAI GCGC 1 cut(s) 520
KflI GGGWCCC 1 cut(s) 172
KroI GCCGGC 1 cut(s) 535
KroNI GCCGGC 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 816
LmnI GCTCC 4 cut(s) 126, 1009, 2332, 2349
Lsp1109I GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
LweI GCATC 3 cut(s) 1033, 1047, 1476
MaeI CTAG 2 cut(s) 344, 2018
MaeII ACGT 4 cut(s) 237, 1497, 1582, 1706
MaeIII GTNAC 4 cut(s) 428, 1666, 1735, 2085
MboII GAAGA 6 cut(s) 19, 47, 299, 959, 1107, 1295
MfeI CAATTG 1 cut(s) 1763
MflI RGATCY 1 cut(s) 1504
MhlI GDGCHC 1 cut(s) 1582
MlyI GAGTC 1 cut(s) 2004
MmeI TCCRAC 4 cut(s) 761, 908, 1538, 2256
Mph1103I ATGCAT 2 cut(s) 505, 1339
MroNI GCCGGC 1 cut(s) 535
MroXI GAANNNNTTC 2 cut(s) 2144, 2192
MseI TTAA 6 cut(s) 855, 996, 1062, 1809, 1850, 2356
MspA1I CMGCKG 1 cut(s) 481
MspI CCGG 4 cut(s) 227, 536, 1221, 1317
MspR9I CCNGG 7 cut(s) 228, 619, 1221, 1317, 1347, 1395, 1746
MunI CAATTG 1 cut(s) 1763
MvaI CCWGG 4 cut(s) 619, 1347, 1395, 1746
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 2 cut(s) 1196, 1787
NaeI GCCGGC 1 cut(s) 537
NciI CCSGG 3 cut(s) 228, 1221, 1317
NdeI CATATG 2 cut(s) 412, 505
NgoMIV GCCGGC 1 cut(s) 535
NlaIV GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
NmeAIII GCCGAG 2 cut(s) 118, 131
NmuCI GTSAC 3 cut(s) 1666, 1735, 2085
NsiI ATGCAT 2 cut(s) 505, 1339
NspI RCATGY 1 cut(s) 634
NspV TTCGAA 1 cut(s) 1804
PaeI GCATGC 1 cut(s) 634
PceI AGGCCT 1 cut(s) 261
PdiI GCCGGC 1 cut(s) 537
PdmI GAANNNNTTC 2 cut(s) 2144, 2192
PfeI GAWTC 7 cut(s) 797, 807, 1270, 1293, 1543, 1944, 2042
PflMI CCANNNNNTGG 1 cut(s) 1442
PfoI TCCNGGA 1 cut(s) 1744
PkrI GCNGC 5 cut(s) 46, 480, 643, 1216, 1486
PleI GAGTC 1 cut(s) 2003
PpsI GAGTC 1 cut(s) 2003
Ppu21I YACGTR 1 cut(s) 1498
PpuMI RGGWCCY 2 cut(s) 172, 862
PshAI GACNNNNGTC 1 cut(s) 651
Psp5II RGGWCCY 2 cut(s) 172, 862
Psp6I CCWGG 4 cut(s) 617, 1345, 1393, 1744
PspFI CCCAGC 3 cut(s) 824, 920, 1186
PspGI CCWGG 4 cut(s) 617, 1345, 1393, 1744
PspN4I GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
PspPI GGNCC 9 cut(s) 172, 848, 862, 881, 977, 1141, 1445, 2129, 2177
PspPPI RGGWCCY 2 cut(s) 172, 862
PsrI GAACNNNNNNTAC 2 cut(s) 1517, 1549
PstI CTGCAG 1 cut(s) 480
PsuI RGATCY 1 cut(s) 1504
SaqAI TTAA 6 cut(s) 855, 996, 1062, 1809, 1850, 2356
SatI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
Sau96I GGNCC 9 cut(s) 172, 848, 862, 881, 977, 1141, 1445, 2129, 2177
ScaI AGTACT 3 cut(s) 746, 790, 910
SchI GAGTC 1 cut(s) 2004
ScrFI CCNGG 7 cut(s) 228, 619, 1221, 1317, 1347, 1395, 1746
SduI GDGCHC 1 cut(s) 1582
SfaNI GCATC 3 cut(s) 1033, 1047, 1476
SfcI CTRYAG 2 cut(s) 476, 1324
SfuI TTCGAA 1 cut(s) 1804
SinI GGWCC 6 cut(s) 172, 862, 881, 1445, 2129, 2177
SmlI CTYRAG 1 cut(s) 1662
SmoI CTYRAG 1 cut(s) 1662
SnaBI TACGTA 1 cut(s) 1498
SphI GCATGC 1 cut(s) 634
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 4 cut(s) 231, 372, 481, 539
SspI AATATT 1 cut(s) 513
SspMI CTAG 2 cut(s) 344, 2018
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 7 cut(s) 226, 617, 1219, 1315, 1345, 1393, 1744
StyI CCWWGG 2 cut(s) 424, 1990
TaaI ACNGT 8 cut(s) 252, 457, 650, 1328, 1624, 1954, 2085, 2318
TaiI ACGT 4 cut(s) 240, 1500, 1585, 1709
TaqI TCGA 5 cut(s) 516, 753, 1162, 1312, 1804
TatI WGTACW 6 cut(s) 252, 744, 788, 908, 1617, 1896
TfiI GAWTC 7 cut(s) 797, 807, 1270, 1293, 1543, 1944, 2042
Tru1I TTAA 6 cut(s) 855, 996, 1062, 1809, 1850, 2356
Tru9I TTAA 6 cut(s) 855, 996, 1062, 1809, 1850, 2356
TscAI CASTG 4 cut(s) 126, 2044, 2074, 2323
TseFI GTSAC 3 cut(s) 1666, 1735, 2085
TseI GCWGC 5 cut(s) 44, 478, 641, 1214, 1484
Tsp45I GTSAC 3 cut(s) 1666, 1735, 2085
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 4 cut(s) 126, 2044, 2074, 2323
Van91I CCANNNNNTGG 1 cut(s) 1442
VpaK11BI GGWCC 6 cut(s) 172, 862, 881, 1445, 2129, 2177
XceI RCATGY 1 cut(s) 634
XcmI CCANNNNNNNNNTGG 1 cut(s) 1973
XmiI GTMKAC 3 cut(s) 247, 459, 1030
XmnI GAANNNNTTC 2 cut(s) 2144, 2192
XspI CTAG 2 cut(s) 344, 2018
ZrmI AGTACT 3 cut(s) 746, 790, 910
Zsp2I ATGCAT 2 cut(s) 505, 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.