MD12G1256900.v1.1

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000523
Physical Location & Seq
Reverse (-)
32435643 .. 32437717
2075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1256900.v1.1.491

Sequence Viewer

Length: 2055 bp
ATGCTTTCGATGGAGAAGGTGACGCAAATGAGGAGATGGGTGATCCTTGGAGTATTAGCACTAGCATTACTTGGTTTGCTCTTCTTCTTCCCTATTAACAATGGTGCATACGAGACGACGAGGATTTCTGGAAGGCGAGCACTCAGGAGCGTTACTGGCAATGAGCTCAACTTCAACCAGATGCTAACAGAGCAAACTCAGAACACGGTAGAGCTGAACAATGGCATTGTCCGGGTCACTTTTTCAAATCCCGGAGGAGACGTCATTGGAATCCAATACAAGGGAATTGACAACTTGCTTGACATCAAAAACCCCCCAAGCAATAGAGGTTACTGGGATCTTGTATGGAAAAACGGCGAGGACAGACTACAAGGAACAACATTTAAGATTGTTACATCGACGGCGGATCAAATAGAGATTTCTTTTAATAAAACTTATGATGTTTCCCTCGGAGATTGGACGCTACCCTTAAATGTTGACAAAAGGTACATAATGCAGCGTGGTCGCGCTGGGTTCTACGCGAATGCCATATACGAGCGTCTAGAAGGGTGGCCGGAATTGCGCATAGATCAAACAAGACTTGCTTTGAAACTTCGACAAGATAAATTTCAGTTCATGGCTATATCAGACGACAGACAAAGATTCATGCCAACAGCCGAAGATCGCAAAGGAGACCACTCTCAGACCCTTGCCTATCGTGAAGCTGTTCTTTTAACGAATCCAAGCAATCCCGAGTTTAAAGGAGAGGTGGATGACAAGTACCAATACTCCAATGATGTCAAAGACATTAAGGTTCACGGTTGGATTAGCACTGACGATGCTATAGGGCTTTGGATGATCACACCTAGTATTGAGTTTCGTTCAGCGGGGCCATTCAAGCAGGAACTTACCTCTCACGTCGGCCCAACTGCCCTCACTGTGTTTCTTAGTAGTCACTACGTCGGCAAAGACGTTGGGTTGACCTTTGCAGACGGTGAGGCATGGAAGAAGGTTTTCGGGCCCGTCTTTGTGTTTCTTAACTCAGACGTTCCAAGCTCGAATGCGTCCTTCTCTCTCTGGGAAAATGCAAAAGAACAGATGTATGAAGAAGTAAAGAGTTGGCCATACAATTTCACTCAGTCCGAAGACTTTCCTAACTCCGATGAACGCGGATCAGTGGCTGGTCAGTTACTAATACGGGATTCGTACATCAACGAGAGCCTAGTCTCAGCAAGTTATGCTTATGTGGGACTGGCTGCGAGCGGATACGCGGGATCATGGCAAACGGAATGCAAGGGTTATCAGTTCTGGACTCAAGCTGACAAGCAAGGTTATTTCTACGTGAAAGATGTTCGACCGGGGAACTATAGTTTGTACGCAACTGTTCCCGGCTTCATTGGGGACTACAAATACGAGTACGATATTACAATTGAAGCAGCAAGTGAAGTCAGTTTAGCTAATCTTACCTTTGAACCTCCGAGAAACGGTCCGACCTTGTGGGAAATCGGCACCCCCGATCGATCAGCCGCGGAGTTCAACATACCGGATCCATATCCAACCCTTGTGAACCGATTGTTCACTGGTAATTACGCAAACAGGTTTAGGCAATATGGCTTGTGGGAACGATACGGAGATTACTACCCTGACCGTGATCTCATCTACACCGTCGGCACTGACAATTACAGAGATGATTGGTTTTACGCTCATGTGACAAGGAACAGAAATGGTACATACGAGGCAACGACATGGCAAATTGTATTTCAACTTGAGAACGTTTGGAACACCGGAAATTATATGCTCCAACTGGCGTTGGCCTCGGCCAACTACGCAGAACTGCAGGTCCGATTCAACAACCGGAATGACGCACCTCATTTTTCGACGGGGCTAATAGGGAAGGACAATGCCATAGCAAGGCATGGAATTCATGGTTTGTACTGGCTCTTCAGTGTCGATGTGCCGAGCTATCGACTTCAACAAGGATACAACACCATATATCTTACTCAATCAAGAAACTGGGGACCTTTCGCAGGACTTATGTACGATTACATCCGACTGGAAGGACCTCCTCCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

685

Amino Acids

78.25

Weight (kDa)

5.26

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 63 - 250 1.9e-78 Rhamnogalacturonate lyase family
fn3_3 PF14686 406 - 476 1.8e-20 Polysaccharide lyase family 4, domain II
CBM-like PF14683 491 - 678 4.6e-47 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
Acc16I TGCGCA 1 cut(s) 563
Acc36I ACCTGC 1 cut(s) 1807
AccB1I GGYRCC 1 cut(s) 1487
AccBSI CCGCTC 1 cut(s) 1242
AccII CGCG 5 cut(s) 507, 521, 1149, 1250, 1508
AciI CCGC 7 cut(s) 404, 866, 1149, 1242, 1250, 1506, 1508
AclI AACGTT 1 cut(s) 1752
AclWI GGATC 7 cut(s) 37, 345, 414, 1159, 1261, 1520, 1533
AcoI YGGCCR 3 cut(s) 551, 1100, 1797
AcsI RAATTY 2 cut(s) 605, 1899
AcuI CTGAAG 1 cut(s) 1906
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 8 cut(s) 488, 761, 1187, 1355, 1397, 1708, 1913, 2018
AfiI CCNNNNNNNGG 4 cut(s) 280, 1463, 1890, 2006
AjiI CACGTC 1 cut(s) 898
AloI GAACNNNNNNTCC 2 cut(s) 1803, 1835
AluBI AGCT 7 cut(s) 166, 214, 704, 1035, 1298, 1436, 1941
AluI AGCT 7 cut(s) 166, 214, 704, 1035, 1298, 1436, 1941
Alw21I GWGCWC 2 cut(s) 142, 168
Alw26I GTCTC 4 cut(s) 107, 252, 666, 1210
AlwI GGATC 7 cut(s) 37, 345, 414, 1159, 1261, 1520, 1533
AlwNI CAGNNNCTG 1 cut(s) 1160
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 7 cut(s) 551, 869, 901, 998, 1100, 1791, 1797
ApaI GGGCCC 1 cut(s) 1002
ApeKI GCWGC 3 cut(s) 496, 1235, 1415
ApoI RAATTY 2 cut(s) 605, 1899
Asp700I GAANNNNTTC 3 cut(s) 705, 992, 1128
AspLEI GCGC 2 cut(s) 509, 564
AspS9I GGNCC 8 cut(s) 869, 902, 998, 999, 1466, 1819, 1997, 2039
AsuC2I CCSGG 4 cut(s) 233, 252, 1338, 1368
AsuHPI GGTGA 3 cut(s) 31, 52, 986
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 4 cut(s) 1466, 1819, 1997, 2039
BaeGI GKGCMC 1 cut(s) 1002
BalI TGGCCA 1 cut(s) 1102
BamHI GGATCC 1 cut(s) 1525
BanI GGYRCC 1 cut(s) 1487
BanII GRGCYC 2 cut(s) 168, 1002
BarI GAAGNNNNNNTAC 2 cut(s) 1904, 1936
BbsI GAAGAC 1 cut(s) 1131
Bbv12I GWGCWC 2 cut(s) 142, 168
BbvI GCAGC 3 cut(s) 508, 1222, 1427
BccI CCATC 2 cut(s) 4, 30
BceAI ACGGC 2 cut(s) 370, 417
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BciVI GTATCC 2 cut(s) 1238, 1952
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 4 cut(s) 233, 252, 1338, 1368
BcoDI GTCTC 4 cut(s) 107, 252, 666, 1210
BfaI CTAG 4 cut(s) 62, 542, 846, 1202
BfmI CTRYAG 3 cut(s) 822, 1345, 1814
BfuAI ACCTGC 1 cut(s) 1807
BfuI GTATCC 2 cut(s) 1238, 1952
BisI GCNGC 4 cut(s) 497, 1236, 1416, 1506
BlsI GCNGC 4 cut(s) 498, 1237, 1417, 1507
Bme1390I CCNGG 4 cut(s) 233, 252, 1338, 1368
Bme18I GGWCC 4 cut(s) 1466, 1819, 1997, 2039
BmeT110I CYCGRG 1 cut(s) 731
BmgBI CACGTC 1 cut(s) 898
BmgT120I GGNCC 8 cut(s) 869, 902, 998, 999, 1466, 1819, 1997, 2039
BmiI GGNNCC 5 cut(s) 870, 1000, 1489, 1527, 1998
BmrFI CCNGG 4 cut(s) 233, 252, 1338, 1368
BmrI ACTGGG 2 cut(s) 343, 2002
BmsI GCATC 2 cut(s) 171, 808
BmuI ACTGGG 2 cut(s) 343, 2002
BpiI GAAGAC 1 cut(s) 1131
BpuEI CTTGAG 2 cut(s) 1278, 1766
BpuMI CCSGG 4 cut(s) 233, 252, 1338, 1368
Bsa29I ATCGAT 1 cut(s) 1498
BsaAI YACGTR 1 cut(s) 1321
BsaBI GATNNNNATC 1 cut(s) 1530
BsaHI GRCGYC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 666
BsaJI CCNNGG 5 cut(s) 46, 448, 1337, 1506, 1794
BsaWI WCCGGW 3 cut(s) 1522, 1763, 1833
BsaXI ACNNNNNCTCC 4 cut(s) 246, 276, 752, 782
Bsc4I CCNNNNNNNGG 4 cut(s) 280, 1463, 1890, 2006
Bse1I ACTGG 8 cut(s) 160, 338, 1236, 1564, 1788, 1919, 1997, 2037
Bse3DI GCAATG 1 cut(s) 166
Bse8I GATNNNNATC 1 cut(s) 1530
BseCI ATCGAT 1 cut(s) 1498
BseDI CCNNGG 5 cut(s) 46, 448, 1337, 1506, 1794
BseGI GGATG 3 cut(s) 757, 840, 2025
BseJI GATNNNNATC 1 cut(s) 1530
BseLI CCNNNNNNNGG 4 cut(s) 280, 1463, 1890, 2006
BseMI GCAATG 1 cut(s) 166
BseMII CTCAG 6 cut(s) 157, 212, 695, 1035, 1130, 1221
BseNI ACTGG 8 cut(s) 160, 338, 1236, 1564, 1788, 1919, 1997, 2037
BseRI GAGGAG 3 cut(s) 46, 270, 2034
BseSI GKGCMC 1 cut(s) 1002
BseXI GCAGC 3 cut(s) 508, 1222, 1427
BseYI CCCAGC 1 cut(s) 509
Bsh1236I CGCG 5 cut(s) 507, 521, 1149, 1250, 1508
Bsh1285I CGRYCG 2 cut(s) 1337, 1498
BshFI GGCC 7 cut(s) 553, 871, 903, 1000, 1102, 1793, 1799
BshNI GGYRCC 1 cut(s) 1487
BshVI ATCGAT 1 cut(s) 1498
BsiEI CGRYCG 2 cut(s) 1337, 1498
BsiHKAI GWGCWC 2 cut(s) 142, 168
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 8 cut(s) 232, 252, 554, 1337, 1368, 1523, 1764, 1834
BslFI GGGAC 3 cut(s) 1242, 1394, 2010
BslI CCNNNNNNNGG 4 cut(s) 280, 1463, 1890, 2006
BsmAI GTCTC 4 cut(s) 107, 252, 666, 1210
BsmBI CGTCTC 2 cut(s) 107, 252
BsmFI GGGAC 3 cut(s) 1242, 1394, 2010
BsmI GAATGC 3 cut(s) 529, 1045, 1274
BsnI GGCC 7 cut(s) 553, 871, 903, 1000, 1102, 1793, 1799
Bso31I GGTCTC 1 cut(s) 666
BsoBI CYCGRG 1 cut(s) 731
Bsp120I GGGCCC 1 cut(s) 998
Bsp1286I GDGCHC 3 cut(s) 142, 168, 1002
BspACI CCGC 7 cut(s) 404, 866, 1149, 1242, 1250, 1506, 1508
BspANI GGCC 7 cut(s) 553, 871, 903, 1000, 1102, 1793, 1799
BspCNI CTCAG 6 cut(s) 156, 211, 694, 1034, 1129, 1220
BspDI ATCGAT 1 cut(s) 1498
BspFNI CGCG 5 cut(s) 507, 521, 1149, 1250, 1508
BspLI GGNNCC 5 cut(s) 870, 1000, 1489, 1527, 1998
BspMAI CTGCAG 1 cut(s) 1818
BspMI ACCTGC 1 cut(s) 1807
BspPI GGATC 7 cut(s) 37, 345, 414, 1159, 1261, 1520, 1533
BspQI GCTCTTC 2 cut(s) 86, 1925
BspT107I GGYRCC 1 cut(s) 1487
BspTNI GGTCTC 1 cut(s) 666
BsrBI CCGCTC 1 cut(s) 1242
BsrDI GCAATG 1 cut(s) 166
BsrI ACTGG 8 cut(s) 160, 338, 1236, 1564, 1788, 1919, 1997, 2037
BssECI CCNNGG 5 cut(s) 46, 448, 1337, 1506, 1794
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 46
Bst4CI ACNGT 8 cut(s) 208, 800, 919, 974, 1363, 1466, 1628, 1645
Bst6I CTCTTC 2 cut(s) 86, 1925
BstACI GRCGYC 1 cut(s) 261
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstBAI YACGTR 1 cut(s) 1321
BstC8I GCNNGC 2 cut(s) 138, 1240
BstDEI CTNAG 7 cut(s) 143, 198, 681, 926, 1021, 1116, 1207
BstDSI CCRYGG 1 cut(s) 1506
BstENI CCTNNNNNAGG 1 cut(s) 2004
BstF5I GGATG 3 cut(s) 757, 840, 2025
BstFNI CGCG 5 cut(s) 507, 521, 1149, 1250, 1508
BstHHI GCGC 2 cut(s) 509, 564
BstMAI GTCTC 4 cut(s) 107, 252, 666, 1210
BstMCI CGRYCG 2 cut(s) 1337, 1498
BstMWI GCNNNNNNNGC 6 cut(s) 156, 190, 559, 877, 1217, 1805
BstSCI CCNGG 4 cut(s) 231, 250, 1336, 1366
BstSFI CTRYAG 3 cut(s) 822, 1345, 1814
BstSLI GKGCMC 1 cut(s) 1002
BstUI CGCG 5 cut(s) 507, 521, 1149, 1250, 1508
BstV1I GCAGC 3 cut(s) 508, 1222, 1427
BstV2I GAAGAC 1 cut(s) 1131
BstX2I RGATCY 2 cut(s) 337, 1525
BstYI RGATCY 2 cut(s) 337, 1525
Bsu15I ATCGAT 1 cut(s) 1498
BsuI GTATCC 2 cut(s) 1238, 1952
BsuRI GGCC 7 cut(s) 553, 871, 903, 1000, 1102, 1793, 1799
BsuTUI ATCGAT 1 cut(s) 1498
BtgI CCRYGG 1 cut(s) 1506
BtrI CACGTC 1 cut(s) 898
BtsCI GGATG 3 cut(s) 757, 840, 2025
BtsIMutI CAGTG 6 cut(s) 810, 915, 1161, 1557, 1650, 1930
BveI ACCTGC 1 cut(s) 1807
Cac8I GCNNGC 2 cut(s) 138, 1240
CaiI CAGNNNCTG 1 cut(s) 1160
CfoI GCGC 2 cut(s) 509, 564
Cfr13I GGNCC 8 cut(s) 869, 902, 998, 999, 1466, 1819, 1997, 2039
Cfr42I CCGCGG 1 cut(s) 1509
ClaI ATCGAT 1 cut(s) 1498
CpoI CGGWCCG 1 cut(s) 1466
CseI GACGC 5 cut(s) 31, 469, 527, 1032, 1850
Csp6I GTAC 8 cut(s) 487, 760, 1186, 1354, 1396, 1707, 1912, 2017
CspI CGGWCCG 1 cut(s) 1466
CviAII CATG 8 cut(s) 616, 646, 981, 1257, 1685, 1725, 1895, 1904
CviQI GTAC 8 cut(s) 487, 760, 1186, 1354, 1396, 1707, 1912, 2017
DdeI CTNAG 7 cut(s) 143, 198, 681, 926, 1021, 1116, 1207
DraI TTTAAA 1 cut(s) 739
EaeI YGGCCR 3 cut(s) 551, 1100, 1797
Eam1104I CTCTTC 2 cut(s) 86, 1925
EarI CTCTTC 2 cut(s) 86, 1925
EciI GGCGGA 1 cut(s) 419
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 46
Eco24I GRGCYC 2 cut(s) 168, 1002
Eco31I GGTCTC 1 cut(s) 666
Eco47I GGWCC 4 cut(s) 1466, 1819, 1997, 2039
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 1906
Eco88I CYCGRG 1 cut(s) 731
EcoICRI GAGCTC 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 2004
EcoO109I RGGNCCY 2 cut(s) 1997, 2039
EcoRI GAATTC 1 cut(s) 1899
EcoT14I CCWWGG 1 cut(s) 46
EcoT38I GRGCYC 2 cut(s) 168, 1002
ErhI CCWWGG 1 cut(s) 46
Esp3I CGTCTC 2 cut(s) 107, 252
FaeI CATG 8 cut(s) 619, 649, 984, 1260, 1688, 1728, 1898, 1907
FalI AAGNNNNNCTT 6 cut(s) 568, 600, 693, 725, 1204, 1236
FaqI GGGAC 3 cut(s) 1242, 1394, 2010
FatI CATG 8 cut(s) 615, 645, 980, 1256, 1684, 1724, 1894, 1903
FauI CCCGC 2 cut(s) 859, 1243
FbaI TGATCA 1 cut(s) 837
Fnu4HI GCNGC 4 cut(s) 497, 1236, 1416, 1506
FokI GGATG 3 cut(s) 764, 847, 2012
FriOI GRGCYC 2 cut(s) 168, 1002
Fsp4HI GCNGC 4 cut(s) 497, 1236, 1416, 1506
FspBI CTAG 4 cut(s) 62, 542, 846, 1202
FspI TGCGCA 1 cut(s) 563
GlaI GCGC 2 cut(s) 508, 563
GluI GCNGC 4 cut(s) 497, 1236, 1416, 1506
GsaI CCCAGC 1 cut(s) 513
HaeIII GGCC 7 cut(s) 553, 871, 903, 1000, 1102, 1793, 1799
HapII CCGG 8 cut(s) 232, 252, 554, 1337, 1368, 1523, 1764, 1834
HgaI GACGC 5 cut(s) 31, 469, 527, 1032, 1850
HhaI GCGC 2 cut(s) 509, 564
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 8 cut(s) 619, 649, 984, 1260, 1688, 1728, 1898, 1907
Hin6I GCGC 2 cut(s) 507, 562
HinP1I GCGC 2 cut(s) 507, 562
HincII GTYRAC 2 cut(s) 478, 960
HindII GTYRAC 2 cut(s) 478, 960
HinfI GANTC 6 cut(s) 270, 642, 718, 1181, 1291, 1824
HpaII CCGG 8 cut(s) 232, 252, 554, 1337, 1368, 1523, 1764, 1834
HphI GGTGA 3 cut(s) 31, 52, 986
Hpy166II GTNNAC 5 cut(s) 478, 796, 960, 1546, 1557
Hpy188III TCNNGA 7 cut(s) 129, 145, 542, 698, 731, 1288, 1986
Hpy8I GTNNAC 5 cut(s) 478, 796, 960, 1546, 1557
Hpy99I CGWCG 6 cut(s) 121, 403, 902, 944, 1649, 1861
HpyAV CCTTC 7 cut(s) 10, 126, 539, 982, 1057, 1867, 2030
HpyCH4III ACNGT 8 cut(s) 208, 800, 919, 974, 1363, 1466, 1628, 1645
HpyCH4IV ACGT 7 cut(s) 261, 897, 939, 951, 1026, 1320, 1752
HpyCH4V TGCA 6 cut(s) 107, 496, 968, 1067, 1272, 1816
HpyF10VI GCNNNNNNNGC 6 cut(s) 156, 190, 559, 877, 1217, 1805
HpyF3I CTNAG 7 cut(s) 143, 198, 681, 926, 1021, 1116, 1207
HpySE526I ACGT 7 cut(s) 261, 897, 939, 951, 1026, 1320, 1752
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 8 cut(s) 619, 649, 984, 1260, 1688, 1728, 1898, 1907
HspAI GCGC 2 cut(s) 507, 562
Ksp22I TGATCA 1 cut(s) 837
KspI CCGCGG 1 cut(s) 1509
LguI GCTCTTC 2 cut(s) 86, 1925
LmnI GCTCC 2 cut(s) 147, 1782
Lsp1109I GCAGC 3 cut(s) 508, 1222, 1427
LweI GCATC 2 cut(s) 171, 808
MaeI CTAG 4 cut(s) 62, 542, 846, 1202
MaeII ACGT 7 cut(s) 261, 897, 939, 951, 1026, 1320, 1752
MaeIII GTNAC 8 cut(s) 19, 151, 235, 329, 391, 932, 1167, 1687
MbiI CCGCTC 1 cut(s) 1242
MboII GAAGA 8 cut(s) 73, 76, 79, 671, 997, 1097, 1136, 1912
MfeI CAATTG 1 cut(s) 1407
MflI RGATCY 2 cut(s) 337, 1525
MhlI GDGCHC 3 cut(s) 142, 168, 1002
MlsI TGGCCA 1 cut(s) 1102
MluNI TGGCCA 1 cut(s) 1102
MlyI GAGTC 1 cut(s) 1285
MmeI TCCRAC 5 cut(s) 782, 1493, 1559, 1804, 2053
Mox20I TGGCCA 1 cut(s) 1102
MroXI GAANNNNTTC 3 cut(s) 705, 992, 1128
MscI TGGCCA 1 cut(s) 1102
MseI TTAA 8 cut(s) 96, 384, 426, 470, 713, 738, 789, 1017
Msp20I TGGCCA 1 cut(s) 1102
MspA1I CMGCKG 2 cut(s) 866, 1508
MspI CCGG 8 cut(s) 232, 252, 554, 1337, 1368, 1523, 1764, 1834
MspR9I CCNGG 4 cut(s) 233, 252, 1338, 1368
MunI CAATTG 1 cut(s) 1407
Mva1269I GAATGC 3 cut(s) 529, 1045, 1274
MvnI CGCG 5 cut(s) 507, 521, 1149, 1250, 1508
MwoI GCNNNNNNNGC 6 cut(s) 156, 190, 559, 877, 1217, 1805
NciI CCSGG 4 cut(s) 233, 252, 1338, 1368
NlaIII CATG 8 cut(s) 619, 649, 984, 1260, 1688, 1728, 1898, 1907
NlaIV GGNNCC 5 cut(s) 870, 1000, 1489, 1527, 1998
NmeAIII GCCGAG 2 cut(s) 1775, 1962
NmuCI GTSAC 4 cut(s) 19, 235, 932, 1687
NsbI TGCGCA 1 cut(s) 563
PciSI GCTCTTC 2 cut(s) 86, 1925
PcsI WCGNNNNNNNCGW 2 cut(s) 948, 1491
PctI GAATGC 3 cut(s) 529, 1045, 1274
PdmI GAANNNNTTC 3 cut(s) 705, 992, 1128
PfeI GAWTC 5 cut(s) 270, 642, 718, 1181, 1824
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 4 cut(s) 498, 1237, 1417, 1507
Ple19I CGATCG 1 cut(s) 1498
PleI GAGTC 1 cut(s) 1285
PpsI GAGTC 1 cut(s) 1285
Ppu21I YACGTR 1 cut(s) 1321
PpuMI RGGWCCY 2 cut(s) 1997, 2039
Psp124BI GAGCTC 1 cut(s) 168
Psp1406I AACGTT 1 cut(s) 1752
Psp5II RGGWCCY 2 cut(s) 1997, 2039
PspFI CCCAGC 1 cut(s) 509
PspN4I GGNNCC 5 cut(s) 870, 1000, 1489, 1527, 1998
PspOMI GGGCCC 1 cut(s) 998
PspPI GGNCC 8 cut(s) 869, 902, 998, 999, 1466, 1819, 1997, 2039
PspPPI RGGWCCY 2 cut(s) 1997, 2039
PstI CTGCAG 1 cut(s) 1818
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 2 cut(s) 337, 1525
PvuI CGATCG 1 cut(s) 1498
RsaI GTAC 8 cut(s) 488, 761, 1187, 1355, 1397, 1708, 1913, 2018
RsaNI GTAC 8 cut(s) 487, 760, 1186, 1354, 1396, 1707, 1912, 2017
Rsr2I CGGWCCG 1 cut(s) 1466
RsrII CGGWCCG 1 cut(s) 1466
SacI GAGCTC 1 cut(s) 168
SacII CCGCGG 1 cut(s) 1509
SapI GCTCTTC 2 cut(s) 86, 1925
SaqAI TTAA 8 cut(s) 96, 384, 426, 470, 713, 738, 789, 1017
SatI GCNGC 4 cut(s) 497, 1236, 1416, 1506
Sau96I GGNCC 8 cut(s) 869, 902, 998, 999, 1466, 1819, 1997, 2039
SchI GAGTC 1 cut(s) 1285
ScrFI CCNGG 4 cut(s) 233, 252, 1338, 1368
SduI GDGCHC 3 cut(s) 142, 168, 1002
SfaNI GCATC 2 cut(s) 171, 808
SfcI CTRYAG 3 cut(s) 822, 1345, 1814
Sfr303I CCGCGG 1 cut(s) 1509
SgrBI CCGCGG 1 cut(s) 1509
SinI GGWCC 4 cut(s) 1466, 1819, 1997, 2039
SmlI CTYRAG 2 cut(s) 1293, 1745
SmoI CTYRAG 2 cut(s) 1293, 1745
SsiI CCGC 7 cut(s) 404, 866, 1149, 1242, 1250, 1506, 1508
SspMI CTAG 4 cut(s) 62, 542, 846, 1202
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 4 cut(s) 231, 250, 1336, 1366
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 8 cut(s) 208, 800, 919, 974, 1363, 1466, 1628, 1645
TaiI ACGT 7 cut(s) 264, 900, 942, 954, 1029, 1323, 1755
TaqI TCGA 9 cut(s) 8, 398, 595, 1037, 1333, 1498, 1856, 1929, 1945
TatI WGTACW 1 cut(s) 1911
TauI GCSGC 1 cut(s) 1508
TfiI GAWTC 5 cut(s) 270, 642, 718, 1181, 1824
Tru1I TTAA 8 cut(s) 96, 384, 426, 470, 713, 738, 789, 1017
Tru9I TTAA 8 cut(s) 96, 384, 426, 470, 713, 738, 789, 1017
TscAI CASTG 6 cut(s) 817, 922, 1161, 1564, 1657, 1930
TseFI GTSAC 4 cut(s) 19, 235, 932, 1687
TseI GCWGC 3 cut(s) 496, 1235, 1415
Tsp45I GTSAC 4 cut(s) 19, 235, 932, 1687
TspDTI ATGAA 6 cut(s) 604, 634, 1098, 1158, 1363, 1892
TspGWI ACGGA 2 cut(s) 1280, 1623
TspRI CASTG 6 cut(s) 817, 922, 1161, 1564, 1657, 1930
VpaK11BI GGWCC 4 cut(s) 1466, 1819, 1997, 2039
XagI CCTNNNNNAGG 1 cut(s) 2004
XapI RAATTY 2 cut(s) 605, 1899
XbaI TCTAGA 1 cut(s) 541
XmnI GAANNNNTTC 3 cut(s) 705, 992, 1128
XspI CTAG 4 cut(s) 62, 542, 846, 1202
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.