Rh2DG038600

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000523
Physical Location & Seq
Forward (+)
2682965 .. 2686796
3832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG038600.1

Sequence Viewer

Length: 2028 bp
ATGAGGAATTCAGGTGGGTGGATTCTGGGGACGTTATTATTTTTGGGTGGTTTTTTCTTTTTCTTTGTTCATGTTGCGGCAAGTTCACCGAGAAGTCATCGACAACTGGCGCAAGATGCGGTCGAGATCAAGATGGTAAAAAATGGAGTTGTGTTGGATAATAAGCTTGTTCAACTGACCTTTTCTAATCCTGGTGGGGATGTTATTGGAATAAGGTATGCAGGAATTGACAACTTACTTGCAATTAACAACAAGGAAGGTGATAGAGGGTACTGGGATGTGGTTTGGAATTACCCGGGAGAGGCGAAAAACACACAATACAGACTACAAGGAACACAATTTAAAGTAATCACGGAGAGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAACGCCAACTCCGGTGTGACAGCTCCGGTGAATGTTGACAGAAGATACATAATGCAGCAAGGACGTTCCGGGTTCTATACCTATGCCATCTTCGAGCGCCTTGAAGGGTGGCCAGGAGGGGACATGGAGACGGTAAGAGTGGCTTACAAGCTTCATCAAGCCAAGTTTCGATATATGGCACTATCGGACACTAGGCAAAGGTTCATGCCAACACCCGATGACCGAGCACGCGGTAGGCCACTTGCATTCAAAGAAGCTGTTCTACTGAACAATGGGACGAGCAGTCCTGAATTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCTCCACCGGTGGGATTTTGGATGATCACACCTAGTGATGAGTTTCGTATAGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTCGCTAACTGTGTTTGCTGCTCATCATTATGTTGGTAAAGAAGTTGGGCTGACATTCCAAGAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTTTATCTATCTTAACTCGGCCAAAGATAATAGTCCTTCAACGATCCGTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCATACAATTTCATTAGTTCTCCCGACTTTCCTTCTTCGGATCAACGTGGATCAGTTTCGGGCCAGTTACTAGTACATGATCGATACATTGAAAGTCTTCCAAGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAGGGAAAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTCATTGAAAATATTAGACCCGGTAAATATAATTTGTATGCATCTGTTCCAGGGATAGTTGGAGACTACAAATATGAGGTTGACATTACAATTGAACCAGGAAGTAAAATCCAACTGGCTAGTATTACTTACAAACCTCCGAGAACCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCAGAGTTCTACATACCGGATCCATATCCAACTCTCACGAACAAGTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAGTACGGGTTGTGGGCACGTTATTATGAGCGTTACCCTAACAATGACCTCATCTACCGTGTTGGTGCTAACAATTACAGTGAGGATTGGTTTTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGCATGTAGGAACTACATGGCAGATACAATTTGAACTCAAGAATGTGGTCAATCCCGGAAACTATACACTTCAACTGGCCTTGGCATCAGCCACTTATGCAGAATTGCAAGTTCGGGTTAACAATTTGAATGCCAAGCCACCTCTTTTTTCGACAGGGCTAATAGGCGACGACAATGCTATAGCGAGACTTGGAATTCATGGCTTATACAGGTTATGGAGTATTGATGTACCAAGCACTCTACTACGCGAAGGAAGCAACACCATCTATCTTACTCAGTCCAGAGATGGCTATTCACCTTTCCAAGGAGTCATGTATGATTATATAAGACTAGAAGGACCTTCTGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

675

Amino Acids

76.5

Weight (kDa)

6.59

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 35 - 234 1e-71 Rhamnogalacturonate lyase family
fn3_3 PF14686 394 - 465 2.2e-24 Polysaccharide lyase family 4, domain II
CBM-like PF14683 478 - 668 4.3e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 627, 1920
AciI CCGC 3 cut(s) 77, 119, 627
AclWI GGATC 6 cut(s) 1003, 1113, 1123, 1222, 1481, 1494
AcoI YGGCCR 5 cut(s) 506, 723, 984, 1064, 1669
AcsI RAATTY 3 cut(s) 7, 686, 1866
AdeI CACNNNGTG 1 cut(s) 809
AfaI GTAC 7 cut(s) 272, 719, 1015, 1140, 1517, 1550, 1902
AfiI CCNNNNNNNGG 4 cut(s) 301, 785, 1424, 1976
AgeI ACCGGT 2 cut(s) 781, 1424
AhdI GACNNNNNGTC 1 cut(s) 678
AhlI ACTAGT 1 cut(s) 1135
AjnI CCWGG 4 cut(s) 190, 508, 1327, 1375
AluBI AGCT 5 cut(s) 166, 419, 547, 653, 1466
AluI AGCT 5 cut(s) 166, 419, 547, 653, 1466
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 3 cut(s) 518, 1335, 1852
AlwI GGATC 6 cut(s) 1003, 1113, 1123, 1222, 1481, 1494
Ama87I CYCGRG 1 cut(s) 295
AoxI GGCC 9 cut(s) 359, 506, 632, 723, 984, 1064, 1126, 1669, 1749
ApeKI GCWGC 4 cut(s) 451, 887, 1196, 1466
ApoI RAATTY 3 cut(s) 7, 686, 1866
AsiGI ACCGGT 2 cut(s) 781, 1424
Asp700I GAANNNNTTC 2 cut(s) 654, 1161
AspLEI GCGC 2 cut(s) 112, 495
AspS9I GGNCC 7 cut(s) 830, 844, 863, 959, 1126, 1427, 2009
AsuC2I CCSGG 6 cut(s) 296, 297, 466, 1203, 1299, 1728
AsuHPI GGTGA 7 cut(s) 78, 272, 379, 436, 841, 947, 1959
AvaI CYCGRG 1 cut(s) 295
AvaII GGWCC 6 cut(s) 830, 844, 863, 959, 1427, 2009
BaeGI GKGCMC 1 cut(s) 1564
BalI TGGCCA 2 cut(s) 508, 1066
BamHI GGATCC 1 cut(s) 1486
BarI GAAGNNNNNNTAC 2 cut(s) 642, 674
BbsI GAAGAC 1 cut(s) 1154
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 4 cut(s) 463, 874, 1183, 1453
BccI CCATC 4 cut(s) 127, 491, 1943, 1952
BcgI CGANNNNNNTGC 2 cut(s) 1829, 1863
BciT130I CCWGG 4 cut(s) 192, 510, 1329, 1377
BciVI GTATCC 1 cut(s) 1232
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 6 cut(s) 296, 297, 466, 1203, 1299, 1728
BcoDI GTCTC 3 cut(s) 518, 1335, 1852
BcuI ACTAGT 1 cut(s) 1135
BfaI CTAG 6 cut(s) 588, 807, 1136, 1398, 1653, 2003
BfmI CTRYAG 2 cut(s) 1467, 1851
BfoI RGCGCY 1 cut(s) 496
BfuI GTATCC 1 cut(s) 1232
BisI GCNGC 5 cut(s) 78, 452, 888, 1197, 1467
BlsI GCNGC 5 cut(s) 79, 453, 889, 1198, 1468
BmcAI AGTACT 1 cut(s) 719
Bme18I GGWCC 6 cut(s) 830, 844, 863, 959, 1427, 2009
BmeRI GACNNNNNGTC 1 cut(s) 678
BmeT110I CYCGRG 1 cut(s) 295
BmgT120I GGNCC 7 cut(s) 830, 844, 863, 959, 1126, 1427, 2009
BmiI GGNNCC 1 cut(s) 1488
BmrI ACTGGG 1 cut(s) 283
BmsI GCATC 3 cut(s) 106, 1328, 1766
BmuI ACTGGG 1 cut(s) 283
BpiI GAAGAC 1 cut(s) 1154
BpuEI CTTGAG 2 cut(s) 1629, 1694
BpuMI CCSGG 6 cut(s) 296, 297, 466, 1203, 1299, 1728
Bsa29I ATCGAT 1 cut(s) 1147
BsaAI YACGTR 1 cut(s) 1186
BsaBI GATNNNNATC 2 cut(s) 711, 1491
BsaJI CCNNGG 6 cut(s) 295, 726, 1166, 1328, 1752, 1975
BsaWI WCCGGW 5 cut(s) 407, 421, 781, 1424, 1483
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 785, 1424, 1976
Bse118I RCCGGY 3 cut(s) 781, 1424, 1671
Bse1I ACTGG 5 cut(s) 111, 278, 1129, 1398, 1752
Bse8I GATNNNNATC 2 cut(s) 711, 1491
BseBI CCWGG 4 cut(s) 192, 510, 1329, 1377
BseCI ATCGAT 1 cut(s) 1147
BseDI CCNNGG 6 cut(s) 295, 726, 1166, 1328, 1752, 1975
BseGI GGATG 4 cut(s) 205, 283, 709, 801
BseJI GATNNNNATC 2 cut(s) 711, 1491
BseLI CCNNNNNNNGG 4 cut(s) 301, 785, 1424, 1976
BseMII CTCAG 2 cut(s) 1961, 2007
BseNI ACTGG 5 cut(s) 111, 278, 1129, 1398, 1752
BseSI GKGCMC 1 cut(s) 1564
BseX3I CGGCCG 1 cut(s) 723
BseXI GCAGC 4 cut(s) 463, 874, 1183, 1453
BsgI GTGCAG 1 cut(s) 1182
Bsh1236I CGCG 2 cut(s) 627, 1920
Bsh1285I CGRYCG 2 cut(s) 123, 726
BshFI GGCC 9 cut(s) 361, 508, 634, 725, 986, 1066, 1128, 1671, 1751
BshTI ACCGGT 2 cut(s) 781, 1424
BshVI ATCGAT 1 cut(s) 1147
BsiEI CGRYCG 2 cut(s) 123, 726
BsiHKAI GWGCWC 1 cut(s) 625
BsiHKCI CYCGRG 1 cut(s) 295
BslFI GGGAC 3 cut(s) 43, 530, 685
BslI CCNNNNNNNGG 4 cut(s) 301, 785, 1424, 1976
BsmAI GTCTC 3 cut(s) 518, 1335, 1852
BsmBI CGTCTC 1 cut(s) 518
BsmFI GGGAC 3 cut(s) 43, 530, 685
BsmI GAATGC 3 cut(s) 641, 1449, 1807
BsnI GGCC 9 cut(s) 361, 508, 634, 725, 986, 1066, 1128, 1671, 1751
BsoBI CYCGRG 1 cut(s) 295
Bsp1286I GDGCHC 2 cut(s) 625, 1564
Bsp1407I TGTACA 1 cut(s) 1515
BspACI CCGC 3 cut(s) 77, 119, 627
BspANI GGCC 9 cut(s) 361, 508, 634, 725, 986, 1066, 1128, 1671, 1751
BspCNI CTCAG 2 cut(s) 1960, 2008
BspDI ATCGAT 1 cut(s) 1147
BspFNI CGCG 2 cut(s) 627, 1920
BspLI GGNNCC 1 cut(s) 1488
BspMAI CTGCAG 1 cut(s) 1471
BspPI GGATC 6 cut(s) 1003, 1113, 1123, 1222, 1481, 1494
BsrFI RCCGGY 3 cut(s) 781, 1424, 1671
BsrGI TGTACA 1 cut(s) 1515
BsrI ACTGG 5 cut(s) 111, 278, 1129, 1398, 1752
BssAI RCCGGY 3 cut(s) 781, 1424, 1671
BssECI CCNNGG 6 cut(s) 295, 726, 1166, 1328, 1752, 1975
BssT1I CCWWGG 3 cut(s) 1166, 1752, 1975
Bst2UI CCWGG 4 cut(s) 192, 510, 1329, 1377
Bst4CI ACNGT 4 cut(s) 529, 880, 1604, 1625
BstAUI TGTACA 1 cut(s) 1515
BstBAI YACGTR 1 cut(s) 1186
BstC8I GCNNGC 2 cut(s) 625, 1673
BstDEI CTNAG 2 cut(s) 1947, 2016
BstENI CCTNNNNNAGG 1 cut(s) 1974
BstF5I GGATG 4 cut(s) 205, 283, 709, 801
BstFNI CGCG 2 cut(s) 627, 1920
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 2 cut(s) 112, 495
BstMAI GTCTC 3 cut(s) 518, 1335, 1852
BstMCI CGRYCG 2 cut(s) 123, 726
BstMWI GCNNNNNNNGC 3 cut(s) 116, 1769, 1926
BstNI CCWGG 4 cut(s) 192, 510, 1329, 1377
BstNSI RCATGY 1 cut(s) 1679
BstSFI CTRYAG 2 cut(s) 1467, 1851
BstSLI GKGCMC 1 cut(s) 1564
BstSNI TACGTA 1 cut(s) 1186
BstUI CGCG 2 cut(s) 627, 1920
BstV1I GCAGC 4 cut(s) 463, 874, 1183, 1453
BstV2I GAAGAC 1 cut(s) 1154
BstX2I RGATCY 1 cut(s) 1486
BstYI RGATCY 1 cut(s) 1486
BstZI CGGCCG 1 cut(s) 723
Bsu15I ATCGAT 1 cut(s) 1147
BsuI GTATCC 1 cut(s) 1232
BsuRI GGCC 9 cut(s) 361, 508, 634, 725, 986, 1066, 1128, 1671, 1751
BsuTUI ATCGAT 1 cut(s) 1147
BtsCI GGATG 4 cut(s) 205, 283, 709, 801
BtsIMutI CAGTG 1 cut(s) 1630
Cac8I GCNNGC 2 cut(s) 625, 1673
CfoI GCGC 2 cut(s) 112, 495
Cfr10I RCCGGY 3 cut(s) 781, 1424, 1671
Cfr13I GGNCC 7 cut(s) 830, 844, 863, 959, 1126, 1427, 2009
Cfr9I CCCGGG 1 cut(s) 295
ClaI ATCGAT 1 cut(s) 1147
Csp6I GTAC 7 cut(s) 271, 718, 1014, 1139, 1516, 1549, 1901
CspAI ACCGGT 2 cut(s) 781, 1424
CviQI GTAC 7 cut(s) 271, 718, 1014, 1139, 1516, 1549, 1901
DdeI CTNAG 2 cut(s) 1947, 2016
DraI TTTAAA 1 cut(s) 343
DraIII CACNNNGTG 1 cut(s) 809
DriI GACNNNNNGTC 1 cut(s) 678
EaeI YGGCCR 5 cut(s) 506, 723, 984, 1064, 1669
EagI CGGCCG 1 cut(s) 723
Eam1105I GACNNNNNGTC 1 cut(s) 678
EclXI CGGCCG 1 cut(s) 723
Eco105I TACGTA 1 cut(s) 1186
Eco130I CCWWGG 3 cut(s) 1166, 1752, 1975
Eco32I GATATC 1 cut(s) 739
Eco47I GGWCC 6 cut(s) 830, 844, 863, 959, 1427, 2009
Eco52I CGGCCG 1 cut(s) 723
Eco88I CYCGRG 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 1974
EcoO109I RGGNCCY 2 cut(s) 844, 2009
EcoRI GAATTC 2 cut(s) 7, 1866
EcoRII CCWGG 4 cut(s) 190, 508, 1327, 1375
EcoRV GATATC 1 cut(s) 739
EcoT14I CCWWGG 3 cut(s) 1166, 1752, 1975
EcoT22I ATGCAT 1 cut(s) 1321
ErhI CCWWGG 3 cut(s) 1166, 1752, 1975
Esp3I CGTCTC 1 cut(s) 518
FalI AAGNNNNNCTT 4 cut(s) 523, 555, 1165, 1197
FaqI GGGAC 3 cut(s) 43, 530, 685
FbaI TGATCA 1 cut(s) 798
Fnu4HI GCNGC 5 cut(s) 78, 452, 888, 1197, 1467
FokI GGATG 4 cut(s) 212, 290, 716, 808
Fsp4HI GCNGC 5 cut(s) 78, 452, 888, 1197, 1467
FspBI CTAG 6 cut(s) 588, 807, 1136, 1398, 1653, 2003
GlaI GCGC 2 cut(s) 111, 494
GluI GCNGC 5 cut(s) 78, 452, 888, 1197, 1467
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 9 cut(s) 361, 508, 634, 725, 986, 1066, 1128, 1671, 1751
HhaI GCGC 2 cut(s) 112, 495
Hin6I GCGC 2 cut(s) 110, 493
HinP1I GCGC 2 cut(s) 110, 493
HincII GTYRAC 3 cut(s) 433, 1360, 1792
HindII GTYRAC 3 cut(s) 433, 1360, 1792
HindIII AAGCTT 2 cut(s) 164, 545
HinfI GANTC 4 cut(s) 22, 1252, 1525, 1980
HpaI GTTAAC 1 cut(s) 1792
HphI GGTGA 7 cut(s) 78, 272, 379, 436, 841, 947, 1959
Hpy166II GTNNAC 5 cut(s) 86, 433, 1016, 1360, 1792
Hpy188I TCNGA 5 cut(s) 583, 959, 1105, 1419, 2017
Hpy188III TCNNGA 8 cut(s) 124, 130, 683, 1088, 1454, 1504, 1711, 1953
Hpy8I GTNNAC 5 cut(s) 86, 433, 1016, 1360, 1792
Hpy99I CGWCG 1 cut(s) 1844
HpyAV CCTTC 8 cut(s) 251, 494, 686, 1011, 1107, 1916, 2000, 2022
HpyCH4III ACNGT 4 cut(s) 529, 880, 1604, 1625
HpyCH4IV ACGT 6 cut(s) 32, 460, 858, 1111, 1185, 1564
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 1769, 1926
HpyF3I CTNAG 2 cut(s) 1947, 2016
HpySE526I ACGT 6 cut(s) 32, 460, 858, 1111, 1185, 1564
HspAI GCGC 2 cut(s) 110, 493
KroI GCCGGC 1 cut(s) 1671
KroNI GCCGGC 1 cut(s) 1673
Ksp22I TGATCA 1 cut(s) 798
KspAI GTTAAC 1 cut(s) 1792
LmnI GCTCC 1 cut(s) 424
Lsp1109I GCAGC 4 cut(s) 463, 874, 1183, 1453
LweI GCATC 3 cut(s) 106, 1328, 1766
MaeI CTAG 6 cut(s) 588, 807, 1136, 1398, 1653, 2003
MaeII ACGT 6 cut(s) 32, 460, 858, 1111, 1185, 1564
MaeIII GTNAC 4 cut(s) 412, 1131, 1577, 1648
MboII GAAGA 5 cut(s) 450, 478, 1061, 1092, 1154
MfeI CAATTG 1 cut(s) 1368
MflI RGATCY 1 cut(s) 1486
MhlI GDGCHC 2 cut(s) 625, 1564
MlsI TGGCCA 2 cut(s) 508, 1066
MluNI TGGCCA 2 cut(s) 508, 1066
MlyI GAGTC 3 cut(s) 1246, 1534, 1989
MmeI TCCRAC 8 cut(s) 135, 734, 841, 1192, 1318, 1414, 1512, 1520
Mox20I TGGCCA 2 cut(s) 508, 1066
Mph1103I ATGCAT 1 cut(s) 1321
MroNI GCCGGC 1 cut(s) 1671
MroXI GAANNNNTTC 2 cut(s) 654, 1161
MscI TGGCCA 2 cut(s) 508, 1066
MseI TTAA 4 cut(s) 246, 342, 978, 1791
MslI CAYNNNNRTG 1 cut(s) 897
Msp20I TGGCCA 2 cut(s) 508, 1066
MspA1I CMGCKG 1 cut(s) 1466
MunI CAATTG 1 cut(s) 1368
Mva1269I GAATGC 3 cut(s) 641, 1449, 1807
MvaI CCWGG 4 cut(s) 192, 510, 1329, 1377
MvnI CGCG 2 cut(s) 627, 1920
MwoI GCNNNNNNNGC 3 cut(s) 116, 1769, 1926
NaeI GCCGGC 1 cut(s) 1673
NciI CCSGG 6 cut(s) 296, 297, 466, 1203, 1299, 1728
NgoMIV GCCGGC 1 cut(s) 1671
NlaIV GGNNCC 1 cut(s) 1488
NmeAIII GCCGAG 3 cut(s) 701, 751, 962
NmuCI GTSAC 2 cut(s) 412, 1648
NsiI ATGCAT 1 cut(s) 1321
NspI RCATGY 1 cut(s) 1679
PctI GAATGC 3 cut(s) 641, 1449, 1807
PdiI GCCGGC 1 cut(s) 1673
PdmI GAANNNNTTC 2 cut(s) 654, 1161
PfeI GAWTC 1 cut(s) 22
PfoI TCCNGGA 1 cut(s) 1726
PinAI ACCGGT 2 cut(s) 781, 1424
PkrI GCNGC 5 cut(s) 79, 453, 889, 1198, 1468
PleI GAGTC 3 cut(s) 1246, 1533, 1988
PpsI GAGTC 3 cut(s) 1246, 1533, 1988
Ppu21I YACGTR 1 cut(s) 1186
PpuMI RGGWCCY 2 cut(s) 844, 2009
Psp5II RGGWCCY 2 cut(s) 844, 2009
Psp6I CCWGG 4 cut(s) 190, 508, 1327, 1375
PspGI CCWGG 4 cut(s) 190, 508, 1327, 1375
PspN4I GGNNCC 1 cut(s) 1488
PspPI GGNCC 7 cut(s) 830, 844, 863, 959, 1126, 1427, 2009
PspPPI RGGWCCY 2 cut(s) 844, 2009
PsrI GAACNNNNNNTAC 4 cut(s) 1414, 1446, 1499, 1531
PstI CTGCAG 1 cut(s) 1471
PsuI RGATCY 1 cut(s) 1486
PvuII CAGCTG 1 cut(s) 1466
RsaI GTAC 7 cut(s) 272, 719, 1015, 1140, 1517, 1550, 1902
RsaNI GTAC 7 cut(s) 271, 718, 1014, 1139, 1516, 1549, 1901
RseI CAYNNNNRTG 1 cut(s) 897
SaqAI TTAA 4 cut(s) 246, 342, 978, 1791
SatI GCNGC 5 cut(s) 78, 452, 888, 1197, 1467
Sau96I GGNCC 7 cut(s) 830, 844, 863, 959, 1126, 1427, 2009
ScaI AGTACT 1 cut(s) 719
SchI GAGTC 3 cut(s) 1246, 1534, 1989
SduI GDGCHC 2 cut(s) 625, 1564
SfaNI GCATC 3 cut(s) 106, 1328, 1766
SfcI CTRYAG 2 cut(s) 1467, 1851
SgrAI CRCCGGYG 1 cut(s) 781
SinI GGWCC 6 cut(s) 830, 844, 863, 959, 1427, 2009
SmaI CCCGGG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 897
SmlI CTYRAG 2 cut(s) 1644, 1709
SmoI CTYRAG 2 cut(s) 1644, 1709
SnaBI TACGTA 1 cut(s) 1186
SpeI ACTAGT 1 cut(s) 1135
SsiI CCGC 3 cut(s) 77, 119, 627
SspI AATATT 1 cut(s) 1291
SspMI CTAG 6 cut(s) 588, 807, 1136, 1398, 1653, 2003
StyI CCWWGG 3 cut(s) 1166, 1752, 1975
TaaI ACNGT 4 cut(s) 529, 880, 1604, 1625
TaiI ACGT 6 cut(s) 35, 463, 861, 1114, 1188, 1567
TaqI TCGA 6 cut(s) 100, 123, 489, 565, 1147, 1823
TaqII GACCGA 1 cut(s) 633
TatI WGTACW 3 cut(s) 717, 1138, 1515
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 4 cut(s) 246, 342, 978, 1791
Tru9I TTAA 4 cut(s) 246, 342, 978, 1791
TscAI CASTG 1 cut(s) 1630
TseFI GTSAC 2 cut(s) 412, 1648
TseI GCWGC 4 cut(s) 451, 887, 1196, 1466
Tsp45I GTSAC 2 cut(s) 412, 1648
TspDTI ATGAA 7 cut(s) 59, 373, 539, 589, 1066, 1278, 1859
TspGWI ACGGA 2 cut(s) 368, 1001
TspMI CCCGGG 1 cut(s) 295
TspRI CASTG 1 cut(s) 1630
VpaK11BI GGWCC 6 cut(s) 830, 844, 863, 959, 1427, 2009
XagI CCTNNNNNAGG 1 cut(s) 1974
XapI RAATTY 3 cut(s) 7, 686, 1866
XceI RCATGY 1 cut(s) 1679
XmaI CCCGGG 1 cut(s) 295
XmnI GAANNNNTTC 2 cut(s) 654, 1161
XspI CTAG 6 cut(s) 588, 807, 1136, 1398, 1653, 2003
ZrmI AGTACT 1 cut(s) 719
Zsp2I ATGCAT 1 cut(s) 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.