Rh2DG038800

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000523
Physical Location & Seq
Forward (+)
2702803 .. 2712613
9811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG038800.1

Sequence Viewer

Length: 1365 bp
ATGGCGGTATCGGACACTAGGCAAAGGTTTATGCCAACAGCCGATTACCGAGCACGCGGTAAGCCACTTGCATTCAAAGAAGCTGTTCTACTGAACAATGAGACGAGCAGTCCTGAATTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCACCACCGGTGGGATTTTGGATGATCACACCTAGTGCTGAGTTTCGTATGGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTGCCTAACTGGGTTTGCTTCTCATCATTATGTTGGTAAAGAAGTTGGGCTGACATTCCAAGAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTATATCTATCTTAACTCGACAGCACGAAACTCAGATAATAGTCCTTCAACGATCCGTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCATACAATTTCATTAGTTCTCACGACTTTCCTTCTTCAGATCAACGTGGATCAGTTTCGGGCCAGTTACTAGTACATGATCGATACACTGAAAGTCTTCCAAGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAGGGAGAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTCATTGAAAATATTAGACCCGGAAGTAAAATCCAATTGGCTAGTGTTACTTACAAACCTCCGAGAACCGGTACCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCGGAGTTCTACATACCGGATCCATATCCAACTCTCATGAACAAGTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAGTACGGGTTGTGGTCACGTTATTATGAGCGTTACCTTAACAATGACCTCATCTACCTTGTTGGTGCTAACAATTACCGTGAGGATTGGTTTTATGCTCAAGTGACTAGAAGTGTAGGAAATGGCCGGTATGTAGGAACTACATGGCAGATACAGTTTGAACTCAAGAATGTGGTCAATCCCGGAAACTATATACTTCAACTGGCCTTGGCATCAGCCACTTTTGCAGAATTGCAAGTTCGGGTTAACAATTTGAATGCCAAGCCACCTCTTTTTTCGACGGGGCTAATAGGCGACGACAATGCTATAGCAAGACTTGGAATTCATGGCATATACTGGTTATGGAGTATTGATGTACCGAGCACTCTACTACGCGAAGGAAGCAACACCATCTATCTTACTCAGTCCAGATCGAGATGGCAATTCACCTTTCCACGGAGTCATATAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

454

Amino Acids

51.78

Weight (kDa)

7.11

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 1 - 44 1.8e-06 Rhamnogalacturonate lyase family
fn3_3 PF14686 207 - 248 4.4e-13 Polysaccharide lyase family 4, domain II
CBM-like PF14683 267 - 441 1.4e-39 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 788
AccB1I GGYRCC 1 cut(s) 788
AccII CGCG 2 cut(s) 57, 1287
AciI CCGC 3 cut(s) 5, 57, 836
AclWI GGATC 5 cut(s) 442, 562, 661, 848, 861
AcoI YGGCCR 3 cut(s) 153, 503, 1036
AcsI RAATTY 2 cut(s) 116, 1233
AcuI CTGAAG 1 cut(s) 525
AdeI CACNNNGTG 1 cut(s) 239
AfaI GTAC 7 cut(s) 149, 454, 579, 790, 884, 917, 1269
AfiI CCNNNNNNNGG 3 cut(s) 215, 785, 1347
AgeI ACCGGT 3 cut(s) 211, 785, 791
AgsI TTSAA 9 cut(s) 76, 122, 268, 444, 488, 725, 1073, 1112, 1168
AhdI GACNNNNNGTC 1 cut(s) 108
AhlI ACTAGT 1 cut(s) 574
AluBI AGCT 2 cut(s) 83, 833
AluI AGCT 2 cut(s) 83, 833
Alw21I GWGCWC 2 cut(s) 55, 1277
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 5 cut(s) 442, 562, 661, 848, 861
AoxI GGCC 5 cut(s) 153, 503, 565, 1036, 1116
ApeKI GCWGC 2 cut(s) 635, 833
ApoI RAATTY 2 cut(s) 116, 1233
AsiGI ACCGGT 3 cut(s) 211, 785, 791
Asp700I GAANNNNTTC 2 cut(s) 84, 600
Asp718I GGTACC 1 cut(s) 788
AspS9I GGNCC 6 cut(s) 260, 274, 293, 389, 565, 794
AsuC2I CCSGG 3 cut(s) 642, 738, 1095
AsuHPI GGTGA 4 cut(s) 198, 271, 377, 1330
AvaII GGWCC 5 cut(s) 260, 274, 293, 389, 794
BalI TGGCCA 1 cut(s) 505
BamHI GGATCC 1 cut(s) 853
BanI GGYRCC 1 cut(s) 788
BarI GAAGNNNNNNTAC 2 cut(s) 72, 104
BbsI GAAGAC 1 cut(s) 593
Bbv12I GWGCWC 2 cut(s) 55, 1277
BbvI GCAGC 2 cut(s) 622, 820
BccI CCATC 2 cut(s) 1310, 1323
BcgI CGANNNNNNTGC 2 cut(s) 1196, 1230
BciVI GTATCC 1 cut(s) 671
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 3 cut(s) 642, 738, 1095
BcoDI GTCTC 1 cut(s) 95
BcuI ACTAGT 1 cut(s) 574
BfaI CTAG 5 cut(s) 18, 237, 575, 759, 1020
BfmI CTRYAG 1 cut(s) 1218
BfuI GTATCC 1 cut(s) 671
BisI GCNGC 2 cut(s) 636, 834
BlsI GCNGC 2 cut(s) 637, 835
BmcAI AGTACT 1 cut(s) 149
Bme1390I CCNGG 3 cut(s) 642, 738, 1095
Bme18I GGWCC 5 cut(s) 260, 274, 293, 389, 794
BmeRI GACNNNNNGTC 1 cut(s) 108
BmgT120I GGNCC 6 cut(s) 260, 274, 293, 389, 565, 794
BmiI GGNNCC 2 cut(s) 790, 855
BmrFI CCNGG 3 cut(s) 642, 738, 1095
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 1133
BmuI ACTGGG 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 593
BpuEI CTTGAG 2 cut(s) 996, 1061
BpuMI CCSGG 3 cut(s) 642, 738, 1095
Bsa29I ATCGAT 1 cut(s) 586
BsaAI YACGTR 1 cut(s) 625
BsaBI GATNNNNATC 2 cut(s) 141, 858
BsaJI CCNNGG 4 cut(s) 156, 605, 1119, 1346
BsaWI WCCGGW 4 cut(s) 211, 785, 791, 850
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 785, 1347
Bse118I RCCGGY 4 cut(s) 211, 785, 791, 1038
Bse1I ACTGG 4 cut(s) 313, 568, 1119, 1253
Bse8I GATNNNNATC 2 cut(s) 141, 858
BseCI ATCGAT 1 cut(s) 586
BseDI CCNNGG 4 cut(s) 156, 605, 1119, 1346
BseGI GGATG 2 cut(s) 139, 231
BseJI GATNNNNATC 2 cut(s) 141, 858
BseLI CCNNNNNNNGG 3 cut(s) 215, 785, 1347
BseMII CTCAG 3 cut(s) 234, 441, 1328
BseNI ACTGG 4 cut(s) 313, 568, 1119, 1253
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 2 cut(s) 622, 820
BsgI GTGCAG 1 cut(s) 621
Bsh1236I CGCG 2 cut(s) 57, 1287
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 5 cut(s) 155, 505, 567, 1038, 1118
BshNI GGYRCC 1 cut(s) 788
BshTI ACCGGT 3 cut(s) 211, 785, 791
BshVI ATCGAT 1 cut(s) 586
BsiEI CGRYCG 1 cut(s) 156
BsiHKAI GWGCWC 2 cut(s) 55, 1277
BsiSI CCGG 8 cut(s) 212, 642, 738, 786, 792, 851, 1039, 1095
BslI CCNNNNNNNGG 3 cut(s) 215, 785, 1347
BsmAI GTCTC 1 cut(s) 95
BsmBI CGTCTC 1 cut(s) 95
BsmI GAATGC 3 cut(s) 71, 816, 1174
BsnI GGCC 5 cut(s) 155, 505, 567, 1038, 1118
Bsp1286I GDGCHC 2 cut(s) 55, 1277
Bsp1407I TGTACA 1 cut(s) 882
BspACI CCGC 3 cut(s) 5, 57, 836
BspANI GGCC 5 cut(s) 155, 505, 567, 1038, 1118
BspCNI CTCAG 3 cut(s) 235, 440, 1327
BspDI ATCGAT 1 cut(s) 586
BspFNI CGCG 2 cut(s) 57, 1287
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 2 cut(s) 790, 855
BspPI GGATC 5 cut(s) 442, 562, 661, 848, 861
BspT107I GGYRCC 1 cut(s) 788
BsrFI RCCGGY 4 cut(s) 211, 785, 791, 1038
BsrGI TGTACA 1 cut(s) 882
BsrI ACTGG 4 cut(s) 313, 568, 1119, 1253
BssAI RCCGGY 4 cut(s) 211, 785, 791, 1038
BssECI CCNNGG 4 cut(s) 156, 605, 1119, 1346
BssT1I CCWWGG 2 cut(s) 605, 1119
Bst4CI ACNGT 2 cut(s) 992, 1068
BstAUI TGTACA 1 cut(s) 882
BstBAI YACGTR 1 cut(s) 625
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 3 cut(s) 243, 427, 1314
BstDSI CCRYGG 1 cut(s) 1346
BstF5I GGATG 2 cut(s) 139, 231
BstFNI CGCG 2 cut(s) 57, 1287
BstMAI GTCTC 1 cut(s) 95
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 1136, 1293
BstSCI CCNGG 3 cut(s) 640, 736, 1093
BstSFI CTRYAG 1 cut(s) 1218
BstSNI TACGTA 1 cut(s) 625
BstUI CGCG 2 cut(s) 57, 1287
BstV1I GCAGC 2 cut(s) 622, 820
BstV2I GAAGAC 1 cut(s) 593
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BstZI CGGCCG 1 cut(s) 153
Bsu15I ATCGAT 1 cut(s) 586
BsuI GTATCC 1 cut(s) 671
BsuRI GGCC 5 cut(s) 155, 505, 567, 1038, 1118
BsuTUI ATCGAT 1 cut(s) 586
BtgI CCRYGG 1 cut(s) 1346
BtsCI GGATG 2 cut(s) 139, 231
BtsIMutI CAGTG 1 cut(s) 591
Cac8I GCNNGC 1 cut(s) 55
CciI TCATGA 1 cut(s) 870
Cfr10I RCCGGY 4 cut(s) 211, 785, 791, 1038
Cfr13I GGNCC 6 cut(s) 260, 274, 293, 389, 565, 794
ClaI ATCGAT 1 cut(s) 586
Csp6I GTAC 7 cut(s) 148, 453, 578, 789, 883, 916, 1268
CspAI ACCGGT 3 cut(s) 211, 785, 791
CviAII CATG 6 cut(s) 179, 581, 657, 871, 1056, 1238
CviQI GTAC 7 cut(s) 148, 453, 578, 789, 883, 916, 1268
DdeI CTNAG 3 cut(s) 243, 427, 1314
DraIII CACNNNGTG 1 cut(s) 239
DriI GACNNNNNGTC 1 cut(s) 108
EaeI YGGCCR 3 cut(s) 153, 503, 1036
EagI CGGCCG 1 cut(s) 153
Eam1105I GACNNNNNGTC 1 cut(s) 108
EclXI CGGCCG 1 cut(s) 153
Eco105I TACGTA 1 cut(s) 625
Eco130I CCWWGG 2 cut(s) 605, 1119
Eco32I GATATC 1 cut(s) 169
Eco47I GGWCC 5 cut(s) 260, 274, 293, 389, 794
Eco52I CGGCCG 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 525
EcoO109I RGGNCCY 1 cut(s) 274
EcoRI GAATTC 1 cut(s) 1233
EcoRV GATATC 1 cut(s) 169
EcoT14I CCWWGG 2 cut(s) 605, 1119
ErhI CCWWGG 2 cut(s) 605, 1119
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 6 cut(s) 182, 584, 660, 874, 1059, 1241
FalI AAGNNNNNCTT 2 cut(s) 604, 636
FatI CATG 6 cut(s) 178, 580, 656, 870, 1055, 1237
FbaI TGATCA 1 cut(s) 228
Fnu4HI GCNGC 2 cut(s) 636, 834
FokI GGATG 2 cut(s) 146, 238
Fsp4HI GCNGC 2 cut(s) 636, 834
FspBI CTAG 5 cut(s) 18, 237, 575, 759, 1020
GluI GCNGC 2 cut(s) 636, 834
HaeIII GGCC 5 cut(s) 155, 505, 567, 1038, 1118
HapII CCGG 8 cut(s) 212, 642, 738, 786, 792, 851, 1039, 1095
Hin1II CATG 6 cut(s) 182, 584, 660, 874, 1059, 1241
HincII GTYRAC 1 cut(s) 1159
HindII GTYRAC 1 cut(s) 1159
HinfI GANTC 3 cut(s) 691, 892, 1351
HpaI GTTAAC 1 cut(s) 1159
HpaII CCGG 8 cut(s) 212, 642, 738, 786, 792, 851, 1039, 1095
HphI GGTGA 4 cut(s) 198, 271, 377, 1330
Hpy166II GTNNAC 2 cut(s) 455, 1159
Hpy188I TCNGA 5 cut(s) 13, 389, 430, 544, 780
Hpy188III TCNNGA 7 cut(s) 113, 527, 821, 871, 1078, 1320, 1326
Hpy8I GTNNAC 2 cut(s) 455, 1159
Hpy99I CGWCG 2 cut(s) 1195, 1211
HpyAV CCTTC 4 cut(s) 116, 450, 546, 1283
HpyCH4III ACNGT 2 cut(s) 992, 1068
HpyCH4IV ACGT 4 cut(s) 288, 550, 624, 931
HpyCH4V TGCA 5 cut(s) 71, 178, 638, 1139, 1147
HpyF10VI GCNNNNNNNGC 2 cut(s) 1136, 1293
HpyF3I CTNAG 3 cut(s) 243, 427, 1314
HpySE526I ACGT 4 cut(s) 288, 550, 624, 931
Hsp92II CATG 6 cut(s) 182, 584, 660, 874, 1059, 1241
KpnI GGTACC 1 cut(s) 792
Ksp22I TGATCA 1 cut(s) 228
KspAI GTTAAC 1 cut(s) 1159
Lsp1109I GCAGC 2 cut(s) 622, 820
LweI GCATC 1 cut(s) 1133
MaeI CTAG 5 cut(s) 18, 237, 575, 759, 1020
MaeII ACGT 4 cut(s) 288, 550, 624, 931
MaeIII GTNAC 5 cut(s) 570, 763, 927, 944, 1015
MboII GAAGA 3 cut(s) 500, 531, 593
MfeI CAATTG 1 cut(s) 752
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 2 cut(s) 55, 1277
MlsI TGGCCA 1 cut(s) 505
MluNI TGGCCA 1 cut(s) 505
MlyI GAGTC 3 cut(s) 685, 901, 1360
MmeI TCCRAC 5 cut(s) 164, 271, 631, 879, 887
Mox20I TGGCCA 1 cut(s) 505
MroXI GAANNNNTTC 2 cut(s) 84, 600
MscI TGGCCA 1 cut(s) 505
MseI TTAA 3 cut(s) 408, 951, 1158
MslI CAYNNNNRTG 1 cut(s) 327
Msp20I TGGCCA 1 cut(s) 505
MspA1I CMGCKG 1 cut(s) 833
MspI CCGG 8 cut(s) 212, 642, 738, 786, 792, 851, 1039, 1095
MspR9I CCNGG 3 cut(s) 642, 738, 1095
MunI CAATTG 1 cut(s) 752
Mva1269I GAATGC 3 cut(s) 71, 816, 1174
MvnI CGCG 2 cut(s) 57, 1287
MwoI GCNNNNNNNGC 2 cut(s) 1136, 1293
NciI CCSGG 3 cut(s) 642, 738, 1095
NlaIII CATG 6 cut(s) 182, 584, 660, 874, 1059, 1241
NlaIV GGNNCC 2 cut(s) 790, 855
NmeAIII GCCGAG 2 cut(s) 131, 181
NmuCI GTSAC 2 cut(s) 927, 1015
PagI TCATGA 1 cut(s) 870
PctI GAATGC 3 cut(s) 71, 816, 1174
PdmI GAANNNNTTC 2 cut(s) 84, 600
PfoI TCCNGGA 1 cut(s) 1093
PinAI ACCGGT 3 cut(s) 211, 785, 791
PkrI GCNGC 2 cut(s) 637, 835
PleI GAGTC 3 cut(s) 685, 900, 1359
PpsI GAGTC 3 cut(s) 685, 900, 1359
Ppu21I YACGTR 1 cut(s) 625
PpuMI RGGWCCY 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 274
PspN4I GGNNCC 2 cut(s) 790, 855
PspPI GGNCC 6 cut(s) 260, 274, 293, 389, 565, 794
PspPPI RGGWCCY 1 cut(s) 274
PsrI GAACNNNNNNTAC 2 cut(s) 866, 898
PsuI RGATCY 1 cut(s) 853
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 7 cut(s) 149, 454, 579, 790, 884, 917, 1269
RsaNI GTAC 7 cut(s) 148, 453, 578, 789, 883, 916, 1268
RseI CAYNNNNRTG 1 cut(s) 327
SaqAI TTAA 3 cut(s) 408, 951, 1158
SatI GCNGC 2 cut(s) 636, 834
Sau96I GGNCC 6 cut(s) 260, 274, 293, 389, 565, 794
ScaI AGTACT 1 cut(s) 149
SchI GAGTC 3 cut(s) 685, 901, 1360
ScrFI CCNGG 3 cut(s) 642, 738, 1095
SduI GDGCHC 2 cut(s) 55, 1277
SfaNI GCATC 1 cut(s) 1133
SfcI CTRYAG 1 cut(s) 1218
SgrAI CRCCGGYG 1 cut(s) 211
SinI GGWCC 5 cut(s) 260, 274, 293, 389, 794
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 2 cut(s) 1011, 1076
SmoI CTYRAG 2 cut(s) 1011, 1076
SnaBI TACGTA 1 cut(s) 625
SpeI ACTAGT 1 cut(s) 574
SsiI CCGC 3 cut(s) 5, 57, 836
SspI AATATT 1 cut(s) 730
SspMI CTAG 5 cut(s) 18, 237, 575, 759, 1020
StyD4I CCNGG 3 cut(s) 640, 736, 1093
StyI CCWWGG 2 cut(s) 605, 1119
TaaI ACNGT 2 cut(s) 992, 1068
TaiI ACGT 4 cut(s) 291, 553, 627, 934
TaqI TCGA 4 cut(s) 413, 586, 1190, 1325
TatI WGTACW 3 cut(s) 147, 577, 882
Tru1I TTAA 3 cut(s) 408, 951, 1158
Tru9I TTAA 3 cut(s) 408, 951, 1158
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 2 cut(s) 927, 1015
TseI GCWGC 2 cut(s) 635, 833
Tsp45I GTSAC 2 cut(s) 927, 1015
TspDTI ATGAA 4 cut(s) 505, 717, 887, 1226
TspGWI ACGGA 2 cut(s) 440, 1363
TspRI CASTG 1 cut(s) 598
VpaK11BI GGWCC 5 cut(s) 260, 274, 293, 389, 794
XapI RAATTY 2 cut(s) 116, 1233
XmnI GAANNNNTTC 2 cut(s) 84, 600
XspI CTAG 5 cut(s) 18, 237, 575, 759, 1020
ZrmI AGTACT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.