FvH4_1g19200

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000523
Physical Location & Seq
Forward (+)
11416854 .. 11421709
4856 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19200.t1

Sequence Viewer

Length: 2214 bp
ATGCAGAAGGCGGGTCGTCGGAGCCTGAAGCATTGGGGTTGGCTTGTTGGGACATTAGCAGCAATGCTTGTTCTGGTTGTTTATCTGGTTGGTGAAAAAAAATACGAGCAGACCACCCCGAAAACCAGTACCCTACTATTAAGAAAAATACTTGAGAGATATCCCAGAACACAACGGCAGGACTTGAACATTCTCAAACCTAGTAGCGGGAGAAACTTTGCTAGACCACCATATCCAGGGATCAACATGGTAACAAATAATAGTGGCCGGAGGATCGTCGCGAGAACAGAAAATCCGAGGGTCAGCATGCTAAGGAGTCAGAGCCAGATTAGGCTGGATAATGGCTTAGTCCAACTCACAGTTTCTCGTCCCGGCGGAGATGTCATTGGTATAAAATACAAAGGAAGTGACAACTTACTTGAACACAAGAATCTACGAAATAATAGAGGGTACTGGGATGTGGTCTGGAACAAACCGGGAGAGAATTTGCATTATGTTGATAAACTACAAGCAACAAAATTTAGAGTTATTATGGCAACGGCAGACCAGGTTGAAATTTCTTTTGTCAAAATATATAATGTTTCCCAGCAGCATAGTGCCCATGTAGTTCCCTTGGATGTTGACAAAAGGTACATAATGCGACGCGGTCGTTCAGGGTTCTATGCCTATGCCATATTCGAGTGCCGTGAAGGTTGGCCGGAAATGAAGATGGAACAAATTAGAATGGTTTACAAGCTTCAACATGAGAAATTTCAGTATATGGTATTATCAGACACTAGGCAAACATTCATGCCAAAAGACAGAGACCGTAAAAACGGTGAGCAACTTGCCTATCCTGAAGCCGTTCGTTTAACCAAACCAAGTAATCGGGCGTTTAAAGGACAGGTCGATGACAAGTACCAGTACTCAATGGAGGACAAAGATAACAAGGTCCATGGATGGATTTCTATAGACAATTCGTTGGGATTCTGGATGATCACACCCAGTGATGAATTCCGTTCTGCTGGACCCATGAAGCAAGGCCTGACATCCCATGTCGGTCCGACTACCCTCTCGATGTTTCTGAGTACTCACTATGCTGGGAAGCAAGCTGGGATAGAATTGAAAAGAGGTGAGGCATGGAAAAAGGTTTTCGGGCCTGTCTTTGTGTATCTCAATTCAGCTCCAAGATCAAACAATTCCACCGGTACACTTTGGAATGATGCCAAGGAGCAGATGGCTGAAGAAGTCAAAAGATGGCCATACAATTTCACTCAATCCGAAGACTTTCCTCCTTCAGATCAACGGGGATCAGTTTCTGGCCGATTAATAGTACACGATCGGTACATGAGTGAGAGCCTTCTTTCGGCAAGTTCTGCTTATATGGGATTAGCTGCACCAGGAGAAGTGGGATCATGGCAAAAGGAAAGCAAGGGTTACCAATTCTGGACTCGAGCTGACAAACAAGGTCATTTCCTCATCAAAGATGTTCGACCTGGGAACTATAGTCTGTATGGTTGGGTTCCAGGCATCGTTGGGGATTACAAATATGAGGCTGATATTGAAATCAAACCAGGCAGTAAAATCCATTTGGCTAATCTTACTTATGAGCCTCAAAGAAATGGTCCTACCTTGTGGGAAATTGGCATCCCCGATCGAAGTGCAGCTGAGTTCTATGTACCGGATCCATATCCAACTCTCATGAACAAATTGTACAACAACGATCACTCGGACAACAGGTTTAGACAATATGGCTTGTGGAAACGTTATGAAGAACTATACCCCGATCATGATCTTATCTACACCATTGGCGTTAACGATTATCGTCATGATTGGTTCTATGCTCATGTCACCAGGAGTACCGGAAAGGAAAGGTATGTAGGAACCACCTGGCAAATTCGATTTAAACTCAACAGTGTCATGAGTCAAGGAAATTATACACTCCAATTAGCACTGGCTTCAGCTACTTCTTCAGAAATACAGGTTCGATTTAACGACCCGAGTGTCAAGCAACCTCATTTTTCGACGGGGCTAATAGGAGGGGACAATGCCATAGCAAGACATGGAATTCATGGTTTGTATTGGTTCTTCAGTGTTAATGTACCAAGTTCTCTGCTGCAAGAAGGAACCAATACAATCTATCTTACTCAGTCAAGAGGTGGAGCACCTTTTCGAGGAGTCATGTATGACTATATTAGACTAGAAGGACCTTCCATTAGACATCCAGACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

738

Amino Acids

84.72

Weight (kDa)

9.51

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 96 - 296 2.5e-74 Rhamnogalacturonate lyase family
fn3_3 PF14686 451 - 523 3.9e-25 Polysaccharide lyase family 4, domain II
CBM-like PF14683 537 - 727 1.7e-50 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1982
AccII CGCG 2 cut(s) 281, 645
AciI CCGC 4 cut(s) 11, 207, 375, 645
AclI AACGTT 1 cut(s) 1744
AclWI GGATC 6 cut(s) 248, 281, 1297, 1399, 1658, 1671
AcoI YGGCCR 4 cut(s) 265, 695, 1238, 1300
AcsI RAATTY 7 cut(s) 484, 518, 555, 749, 992, 1875, 2046
AcuI CTGAAG 7 cut(s) 47, 858, 1242, 1260, 1923, 1935, 2053
AdeI CACNNNGTG 1 cut(s) 986
AfiI CCNNNNNNNGG 4 cut(s) 206, 236, 1345, 2153
AgeI ACCGGT 1 cut(s) 1184
AgsI TTSAA 6 cut(s) 187, 422, 554, 740, 1105, 1544
AjnI CCWGG 8 cut(s) 235, 546, 1378, 1474, 1504, 1552, 1832, 1868
AloI GAACNNNNNNTCC 2 cut(s) 277, 309
AluBI AGCT 7 cut(s) 736, 1091, 1163, 1373, 1436, 1646, 1943
AluI AGCT 7 cut(s) 736, 1091, 1163, 1373, 1436, 1646, 1943
Alw21I GWGCWC 1 cut(s) 2146
Alw26I GTCTC 1 cut(s) 798
AlwI GGATC 6 cut(s) 248, 281, 1297, 1399, 1658, 1671
AlwNI CAGNNNCTG 1 cut(s) 1298
Ama87I CYCGRG 2 cut(s) 1431, 1978
AoxI GGCC 6 cut(s) 265, 695, 1021, 1136, 1238, 1300
ApeKI GCWGC 5 cut(s) 59, 589, 1373, 1643, 2095
ApoI RAATTY 7 cut(s) 484, 518, 555, 749, 992, 1875, 2046
ArsI GACNNNNNNTTYG 2 cut(s) 1588, 1620
AseI ATTAAT 1 cut(s) 1307
AsiGI ACCGGT 1 cut(s) 1184
Asp700I GAANNNNTTC 2 cut(s) 843, 1266
AspS9I GGNCC 6 cut(s) 931, 1007, 1040, 1136, 1604, 2186
AsuC2I CCSGG 2 cut(s) 372, 477
AsuHPI GGTGA 4 cut(s) 104, 830, 1124, 1822
AvaI CYCGRG 2 cut(s) 1431, 1978
AvaII GGWCC 5 cut(s) 931, 1007, 1040, 1604, 2186
BaeGI GKGCMC 1 cut(s) 601
BaeI ACNNNNGTAYC 2 cut(s) 1315, 1348
BalI TGGCCA 1 cut(s) 1240
BamHI GGATCC 1 cut(s) 1663
BarI GAAGNNNNNNTAC 2 cut(s) 2051, 2083
BbsI GAAGAC 1 cut(s) 1269
Bbv12I GWGCWC 1 cut(s) 2146
BbvI GCAGC 5 cut(s) 71, 601, 1360, 1655, 2082
BccI CCATC 4 cut(s) 703, 933, 1210, 1230
BceAI ACGGC 4 cut(s) 191, 555, 669, 827
BcgI CGANNNNNNTGC 2 cut(s) 1622, 1656
BciT130I CCWGG 8 cut(s) 237, 548, 1380, 1476, 1506, 1554, 1834, 1870
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 2 cut(s) 372, 477
BcoDI GTCTC 1 cut(s) 798
BfaI CTAG 4 cut(s) 201, 222, 777, 2180
BfmI CTRYAG 2 cut(s) 948, 1483
BisI GCNGC 5 cut(s) 60, 590, 1374, 1644, 2096
BlsI GCNGC 5 cut(s) 61, 591, 1375, 1645, 2097
BmcAI AGTACT 2 cut(s) 905, 1069
Bme18I GGWCC 5 cut(s) 931, 1007, 1040, 1604, 2186
BmeT110I CYCGRG 2 cut(s) 1431, 1978
BmgT120I GGNCC 6 cut(s) 931, 1007, 1040, 1136, 1604, 2186
BmiI GGNNCC 6 cut(s) 23, 1009, 1503, 1665, 1864, 2107
BmrI ACTGGG 2 cut(s) 463, 978
BmsI GCATC 3 cut(s) 1192, 1518, 1635
BmuI ACTGGG 2 cut(s) 463, 978
BpiI GAAGAC 1 cut(s) 1269
Bpu10I CCTNAGC 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 2 cut(s) 372, 477
BsaBI GATNNNNATC 2 cut(s) 1668, 1770
BsaI GGTCTC 1 cut(s) 798
BsaJI CCNNGG 6 cut(s) 236, 296, 612, 934, 1206, 1475
BsaWI WCCGGW 3 cut(s) 1184, 1660, 1841
Bsc4I CCNNNNNNNGG 4 cut(s) 206, 236, 1345, 2153
Bse118I RCCGGY 1 cut(s) 1184
Bse1I ACTGG 5 cut(s) 126, 458, 901, 984, 1938
Bse3DI GCAATG 1 cut(s) 69
Bse8I GATNNNNATC 2 cut(s) 1668, 1770
BseBI CCWGG 8 cut(s) 237, 548, 1380, 1476, 1506, 1554, 1834, 1870
BseDI CCNNGG 6 cut(s) 236, 296, 612, 934, 1206, 1475
BseGI GGATG 7 cut(s) 463, 622, 944, 978, 1028, 1626, 2200
BseJI GATNNNNATC 2 cut(s) 1668, 1770
BseLI CCNNNNNNNGG 4 cut(s) 206, 236, 1345, 2153
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 3 cut(s) 1055, 1638, 2141
BseNI ACTGG 5 cut(s) 126, 458, 901, 984, 1938
BseRI GAGGAG 1 cut(s) 2169
BseSI GKGCMC 1 cut(s) 601
BseXI GCAGC 5 cut(s) 71, 601, 1360, 1655, 2082
BseYI CCCAGC 3 cut(s) 585, 1079, 1091
BsgI GTGCAG 2 cut(s) 1359, 1662
Bsh1236I CGCG 2 cut(s) 281, 645
Bsh1285I CGRYCG 3 cut(s) 649, 1321, 1636
BshFI GGCC 6 cut(s) 267, 697, 1023, 1138, 1240, 1302
BshTI ACCGGT 1 cut(s) 1184
BsiEI CGRYCG 3 cut(s) 649, 1321, 1636
BsiHKAI GWGCWC 1 cut(s) 2146
BsiHKCI CYCGRG 2 cut(s) 1431, 1978
BsiSI CCGG 7 cut(s) 268, 372, 476, 698, 1185, 1661, 1842
BslFI GGGAC 3 cut(s) 64, 354, 2036
BslI CCNNNNNNNGG 4 cut(s) 206, 236, 1345, 2153
BsmAI GTCTC 1 cut(s) 798
BsmFI GGGAC 3 cut(s) 64, 354, 2036
BsnI GGCC 6 cut(s) 267, 697, 1023, 1138, 1240, 1302
Bso31I GGTCTC 1 cut(s) 798
BsoBI CYCGRG 2 cut(s) 1431, 1978
Bsp1286I GDGCHC 2 cut(s) 601, 2146
Bsp1407I TGTACA 1 cut(s) 1692
Bsp19I CCATGG 1 cut(s) 934
Bsp68I TCGCGA 1 cut(s) 281
BspACI CCGC 4 cut(s) 11, 207, 375, 645
BspANI GGCC 6 cut(s) 267, 697, 1023, 1138, 1240, 1302
BspCNI CTCAG 3 cut(s) 1056, 1639, 2140
BspFNI CGCG 2 cut(s) 281, 645
BspHI TCATGA 4 cut(s) 1680, 1768, 1807, 1899
BspLI GGNNCC 6 cut(s) 23, 1009, 1503, 1665, 1864, 2107
BspPI GGATC 6 cut(s) 248, 281, 1297, 1399, 1658, 1671
BspTNI GGTCTC 1 cut(s) 798
BsrDI GCAATG 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 1184
BsrGI TGTACA 1 cut(s) 1692
BsrI ACTGG 5 cut(s) 126, 458, 901, 984, 1938
BssAI RCCGGY 1 cut(s) 1184
BssECI CCNNGG 6 cut(s) 236, 296, 612, 934, 1206, 1475
BssT1I CCWWGG 3 cut(s) 612, 934, 1206
Bst2UI CCWGG 8 cut(s) 237, 548, 1380, 1476, 1506, 1554, 1834, 1870
Bst4CI ACNGT 4 cut(s) 361, 809, 818, 1895
BstAPI GCANNNNNTGC 1 cut(s) 1355
BstAUI TGTACA 1 cut(s) 1692
BstC8I GCNNGC 2 cut(s) 308, 1089
BstDEI CTNAG 5 cut(s) 311, 346, 1064, 1647, 2127
BstDSI CCRYGG 1 cut(s) 934
BstEII GGTNACC 1 cut(s) 1415
BstENI CCTNNNNNAGG 1 cut(s) 2151
BstF5I GGATG 7 cut(s) 463, 622, 944, 978, 1028, 1626, 2200
BstFNI CGCG 2 cut(s) 281, 645
BstMAI GTCTC 1 cut(s) 798
BstMCI CGRYCG 3 cut(s) 649, 1321, 1636
BstMWI GCNNNNNNNGC 1 cut(s) 1355
BstNI CCWGG 8 cut(s) 237, 548, 1380, 1476, 1506, 1554, 1834, 1870
BstNSI RCATGY 1 cut(s) 310
BstPI GGTNACC 1 cut(s) 1415
BstSFI CTRYAG 2 cut(s) 948, 1483
BstSLI GKGCMC 1 cut(s) 601
BstUI CGCG 2 cut(s) 281, 645
BstV1I GCAGC 5 cut(s) 71, 601, 1360, 1655, 2082
BstV2I GAAGAC 1 cut(s) 1269
BstX2I RGATCY 1 cut(s) 1663
BstYI RGATCY 1 cut(s) 1663
BsuRI GGCC 6 cut(s) 267, 697, 1023, 1138, 1240, 1302
BtgI CCRYGG 1 cut(s) 934
BtsCI GGATG 7 cut(s) 463, 622, 944, 978, 1028, 1626, 2200
BtsIMutI CAGTG 4 cut(s) 991, 1900, 1931, 2077
BtuMI TCGCGA 1 cut(s) 281
Cac8I GCNNGC 2 cut(s) 308, 1089
CaiI CAGNNNCTG 1 cut(s) 1298
CciI TCATGA 4 cut(s) 1680, 1768, 1807, 1899
Cfr10I RCCGGY 1 cut(s) 1184
Cfr13I GGNCC 6 cut(s) 931, 1007, 1040, 1136, 1604, 2186
CpoI CGGWCCG 1 cut(s) 1040
CseI GACGC 1 cut(s) 651
CsiI ACCWGGT 1 cut(s) 546
CspAI ACCGGT 1 cut(s) 1184
CspI CGGWCCG 1 cut(s) 1040
DdeI CTNAG 5 cut(s) 311, 346, 1064, 1647, 2127
DraI TTTAAA 2 cut(s) 877, 1885
DraIII CACNNNGTG 1 cut(s) 986
DrdI GACNNNNNNGTC 1 cut(s) 1982
DseDI GACNNNNNNGTC 1 cut(s) 1982
EaeI YGGCCR 4 cut(s) 265, 695, 1238, 1300
EciI GGCGGA 1 cut(s) 390
Eco130I CCWWGG 3 cut(s) 612, 934, 1206
Eco147I AGGCCT 1 cut(s) 1023
Eco31I GGTCTC 1 cut(s) 798
Eco32I GATATC 1 cut(s) 161
Eco47I GGWCC 5 cut(s) 931, 1007, 1040, 1604, 2186
Eco57I CTGAAG 7 cut(s) 47, 858, 1242, 1260, 1923, 1935, 2053
Eco88I CYCGRG 2 cut(s) 1431, 1978
Eco91I GGTNACC 1 cut(s) 1415
EcoNI CCTNNNNNAGG 1 cut(s) 2151
EcoO109I RGGNCCY 1 cut(s) 2186
EcoO65I GGTNACC 1 cut(s) 1415
EcoRI GAATTC 2 cut(s) 992, 2046
EcoRII CCWGG 8 cut(s) 235, 546, 1378, 1474, 1504, 1552, 1832, 1868
EcoRV GATATC 1 cut(s) 161
EcoT14I CCWWGG 3 cut(s) 612, 934, 1206
ErhI CCWWGG 3 cut(s) 612, 934, 1206
FalI AAGNNNNNCTT 2 cut(s) 1342, 1374
FaqI GGGAC 3 cut(s) 64, 354, 2036
FauI CCCGC 2 cut(s) 4, 200
FbaI TGATCA 1 cut(s) 975
Fnu4HI GCNGC 5 cut(s) 60, 590, 1374, 1644, 2096
FokI GGATG 7 cut(s) 470, 629, 951, 985, 1015, 1613, 2187
Fsp4HI GCNGC 5 cut(s) 60, 590, 1374, 1644, 2096
FspBI CTAG 4 cut(s) 201, 222, 777, 2180
GluI GCNGC 5 cut(s) 60, 590, 1374, 1644, 2096
GsaI CCCAGC 3 cut(s) 589, 1083, 1095
HaeIII GGCC 6 cut(s) 267, 697, 1023, 1138, 1240, 1302
HapII CCGG 7 cut(s) 268, 372, 476, 698, 1185, 1661, 1842
HgaI GACGC 1 cut(s) 651
HincII GTYRAC 2 cut(s) 622, 1795
HindII GTYRAC 2 cut(s) 622, 1795
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 6 cut(s) 316, 430, 966, 1429, 1903, 2157
HpaI GTTAAC 1 cut(s) 1795
HpaII CCGG 7 cut(s) 268, 372, 476, 698, 1185, 1661, 1842
HphI GGTGA 4 cut(s) 104, 830, 1124, 1822
Hpy166II GTNNAC 5 cut(s) 622, 730, 1190, 1315, 1795
Hpy8I GTNNAC 5 cut(s) 622, 730, 1190, 1315, 1795
Hpy99I CGWCG 4 cut(s) 21, 281, 645, 2008
HpyAV CCTTC 6 cut(s) 683, 1284, 1349, 2096, 2177, 2199
HpyCH4III ACNGT 4 cut(s) 361, 809, 818, 1895
HpyCH4IV ACGT 1 cut(s) 1744
HpyCH4V TGCA 5 cut(s) 4, 490, 1376, 1643, 2098
HpyF10VI GCNNNNNNNGC 1 cut(s) 1355
HpyF3I CTNAG 5 cut(s) 311, 346, 1064, 1647, 2127
HpySE526I ACGT 1 cut(s) 1744
Ksp22I TGATCA 1 cut(s) 975
KspAI GTTAAC 1 cut(s) 1795
LmnI GCTCC 4 cut(s) 21, 1168, 1210, 2141
Lsp1109I GCAGC 5 cut(s) 71, 601, 1360, 1655, 2082
LweI GCATC 3 cut(s) 1192, 1518, 1635
MabI ACCWGGT 1 cut(s) 546
MaeI CTAG 4 cut(s) 201, 222, 777, 2180
MaeII ACGT 1 cut(s) 1744
MaeIII GTNAC 4 cut(s) 250, 407, 1415, 1828
MboII GAAGA 6 cut(s) 718, 1235, 1274, 1763, 1941, 2059
MflI RGATCY 1 cut(s) 1663
MhlI GDGCHC 2 cut(s) 601, 2146
MlsI TGGCCA 1 cut(s) 1240
MluNI TGGCCA 1 cut(s) 1240
MlyI GAGTC 4 cut(s) 325, 1423, 1912, 2166
MmeI TCCRAC 3 cut(s) 376, 1067, 1697
Mox20I TGGCCA 1 cut(s) 1240
MroXI GAANNNNTTC 2 cut(s) 843, 1266
MscI TGGCCA 1 cut(s) 1240
MseI TTAA 8 cut(s) 140, 851, 876, 1307, 1794, 1884, 1971, 2076
Msp20I TGGCCA 1 cut(s) 1240
MspA1I CMGCKG 1 cut(s) 1646
MspI CCGG 7 cut(s) 268, 372, 476, 698, 1185, 1661, 1842
MvaI CCWGG 8 cut(s) 237, 548, 1380, 1476, 1506, 1554, 1834, 1870
MvnI CGCG 2 cut(s) 281, 645
MwoI GCNNNNNNNGC 1 cut(s) 1355
NciI CCSGG 2 cut(s) 372, 477
NcoI CCATGG 1 cut(s) 934
NlaIV GGNNCC 6 cut(s) 23, 1009, 1503, 1665, 1864, 2107
NmuCI GTSAC 2 cut(s) 407, 1828
NruI TCGCGA 1 cut(s) 281
NspI RCATGY 1 cut(s) 310
PaeI GCATGC 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 1431
PagI TCATGA 4 cut(s) 1680, 1768, 1807, 1899
PceI AGGCCT 1 cut(s) 1023
PdmI GAANNNNTTC 2 cut(s) 843, 1266
PfeI GAWTC 2 cut(s) 430, 966
PflFI GACNNNGTC 1 cut(s) 645
PinAI ACCGGT 1 cut(s) 1184
PkrI GCNGC 5 cut(s) 61, 591, 1375, 1645, 2097
Ple19I CGATCG 2 cut(s) 1321, 1636
PleI GAGTC 4 cut(s) 324, 1423, 1911, 2165
PpsI GAGTC 4 cut(s) 324, 1423, 1911, 2165
PpuMI RGGWCCY 1 cut(s) 2186
PshBI ATTAAT 1 cut(s) 1307
Psp1406I AACGTT 1 cut(s) 1744
Psp5II RGGWCCY 1 cut(s) 2186
Psp6I CCWGG 8 cut(s) 235, 546, 1378, 1474, 1504, 1552, 1832, 1868
PspEI GGTNACC 1 cut(s) 1415
PspFI CCCAGC 3 cut(s) 585, 1079, 1091
PspGI CCWGG 8 cut(s) 235, 546, 1378, 1474, 1504, 1552, 1832, 1868
PspN4I GGNNCC 6 cut(s) 23, 1009, 1503, 1665, 1864, 2107
PspPI GGNCC 6 cut(s) 931, 1007, 1040, 1136, 1604, 2186
PspPPI RGGWCCY 1 cut(s) 2186
PspXI VCTCGAGB 1 cut(s) 1431
PsrI GAACNNNNNNTAC 2 cut(s) 1676, 1708
PstNI CAGNNNCTG 1 cut(s) 1298
PsuI RGATCY 1 cut(s) 1663
PsyI GACNNNGTC 1 cut(s) 645
PvuI CGATCG 2 cut(s) 1321, 1636
PvuII CAGCTG 1 cut(s) 1646
RruI TCGCGA 1 cut(s) 281
Rsr2I CGGWCCG 1 cut(s) 1040
RsrII CGGWCCG 1 cut(s) 1040
SaqAI TTAA 8 cut(s) 140, 851, 876, 1307, 1794, 1884, 1971, 2076
SatI GCNGC 5 cut(s) 60, 590, 1374, 1644, 2096
Sau96I GGNCC 6 cut(s) 931, 1007, 1040, 1136, 1604, 2186
ScaI AGTACT 2 cut(s) 905, 1069
SchI GAGTC 4 cut(s) 325, 1423, 1912, 2166
SduI GDGCHC 2 cut(s) 601, 2146
SexAI ACCWGGT 1 cut(s) 546
SfaNI GCATC 3 cut(s) 1192, 1518, 1635
SfcI CTRYAG 2 cut(s) 948, 1483
Sfr274I CTCGAG 1 cut(s) 1431
SinI GGWCC 5 cut(s) 931, 1007, 1040, 1604, 2186
SlaI CTCGAG 1 cut(s) 1431
SmlI CTYRAG 2 cut(s) 152, 1431
SmoI CTYRAG 2 cut(s) 152, 1431
SphI GCATGC 1 cut(s) 310
SseBI AGGCCT 1 cut(s) 1023
SsiI CCGC 4 cut(s) 11, 207, 375, 645
SspMI CTAG 4 cut(s) 201, 222, 777, 2180
StuI AGGCCT 1 cut(s) 1023
StyI CCWWGG 3 cut(s) 612, 934, 1206
TaaI ACNGT 4 cut(s) 361, 809, 818, 1895
TaiI ACGT 1 cut(s) 1747
TaqII GACCGA 1 cut(s) 1028
TatI WGTACW 4 cut(s) 903, 1067, 1312, 1692
TfiI GAWTC 2 cut(s) 430, 966
Tru1I TTAA 8 cut(s) 140, 851, 876, 1307, 1794, 1884, 1971, 2076
Tru9I TTAA 8 cut(s) 140, 851, 876, 1307, 1794, 1884, 1971, 2076
TscAI CASTG 4 cut(s) 991, 1900, 1938, 2077
TseFI GTSAC 2 cut(s) 407, 1828
TseI GCWGC 5 cut(s) 59, 589, 1373, 1643, 2095
Tsp45I GTSAC 2 cut(s) 407, 1828
TspDTI ATGAA 7 cut(s) 719, 778, 1005, 1028, 1697, 1764, 2039
TspGWI ACGGA 1 cut(s) 986
TspRI CASTG 4 cut(s) 991, 1900, 1938, 2077
Tth111I GACNNNGTC 1 cut(s) 645
VpaK11BI GGWCC 5 cut(s) 931, 1007, 1040, 1604, 2186
VspI ATTAAT 1 cut(s) 1307
XagI CCTNNNNNAGG 1 cut(s) 2151
XapI RAATTY 7 cut(s) 484, 518, 555, 749, 992, 1875, 2046
XceI RCATGY 1 cut(s) 310
XcmI CCANNNNNNNNNTGG 1 cut(s) 1213
XhoI CTCGAG 1 cut(s) 1431
XmnI GAANNNNTTC 2 cut(s) 843, 1266
XspI CTAG 4 cut(s) 201, 222, 777, 2180
ZrmI AGTACT 2 cut(s) 905, 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.