RchiOBHm_Chr2g0088481

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000523
Physical Location & Seq
Forward (+)
3017867 .. 3019903
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46380

Sequence Viewer

Length: 2037 bp
ATGAGGAATTCAGGTGGGTGGATTCTGGGGACGTTATTATTTTTGGGTGGTTTTTTCTTTGTTCATGCTGCGGCAAGTTCACCGAGAAGTCATCGACTACTGGCGCAAGATGCGGTCGAGATCAAGATGGTAAAAAATGGAGTTGTGTTGGATAATAAGCTTGTTCAACTAACTTTCTCTAATCCTGGTGGGGATGTTATTGGAATAAGCTATGGAGGAATTGACAACTTACTTGAAATTAACAACAAGGAAGGTAATAGAGGGTACTGGGATGTGGTTTGGAATGACCCGGGAGAGACGGAAAACACACAATACAGACTACAAGGAACACAATTTAAAGTAATCACGGAGAGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAATGCCAAACTCCAGCGGGGTGTGACAGTTCCGGTGAATGTTGACAGAAGATTCATAATGCAGCAAGGACGTTCCGGGTTCTATACCTATGCCATCTTCGAGCGCCTTGAAGGGTGGCCAGGAGGGGACATGGACACAGTTAGAGTGGCTTTCAAGCTTCAGCAAGCCAAGTTTCGATATATGGCGCTCTCGGACACTAGGCAAAGGTTCATGCCAACAGCCGATGACCGAGCACGCGGTAGGCCACTTGCATTCAAAGAAGCTGTTCTACTGAACAATGAGACGAGCAATCCTGAATTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCACCACCGGTGGGATTTTGGATGATCACACCAAGTGCTGAGTTTCGTATGGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTGCCTAACTGGGTTTGCTTCTCATCATTATGTTGGTAAAGAAGTTGGGCTGAAATTCCAAGAGGGTGAGGTATGGAAAAAGGTTTTCGGACCTGTCTTTATCTATCTTAACTCGGCAGCACGAAACTCAGCTAATAATCCTTCAACGATCCGTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCGTACAATTTCATTAGTTCTCCAGACTTTCCTTCTTCGGATCAACGTGGATCAGTTTCGGGCCAGTTACTAGTACATGATCGATACAATGAAAGTCTTCCAAGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAGGGAAAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTCATTGAAAATATTAGACCCGGTAACTATAATTTGTATGCATCTGTTCCAGGGATAGTTGGAGAGTACAAATATGAGGTTGAAATTACAATTGAACCAGGAAGTAAAATCCAATTGGCTAGTATTACTTACAAACCTCCGAGAACCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCGGAGTTCTACATTCCGGATCCATATCCAACTCTCATGAACAAGTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAGTACGGGTTGTGGTCACATTATTATGATCGTTACCCTAACAATGACCTCATCTACCGTGTTGGTGCTAGCAATTACCGTGAGGATTGGTTTTATGCTCAGGTGACTAGAAGTGTAGGAAATGGCCGGTATGTAGGAACTACGTGGCAGATACAGTTTGAACTCAAGAATGTGGTCAATCCCGGAAACTATACACTTCAACTGGCCTTGGCATCAGCCACTTATGCAGAATTGCAAGTTCGGGTTAACAATTTGAATGCCAAGCCACCTCTTTTTTCGACTGGGCTAATAGGCAGCGACAATGCTATAGCAAGAGTTGGAATTCATGGCTTATACTGGTTATGGAGTATTGATGTACCAAGCACTCTACTACGCGAAGGAAGCAACACCATTTATCTAACTCAGTCCAGAGATGGCAATTCACCTTTCCACGGAGTCATGTATGATTATATAAGACTAGAAGGACCTTCTGAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

678

Amino Acids

76.81

Weight (kDa)

6.42

Isoelectric Point (pI)

32.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 35 - 234 1.8e-74 Rhamnogalacturonate lyase family
fn3_3 PF14686 397 - 468 1.4e-23 Polysaccharide lyase family 4, domain II
CBM-like PF14683 481 - 671 1.2e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 627, 1929
AccIII TCCGGA 1 cut(s) 1492
AciI CCGC 5 cut(s) 71, 113, 406, 627, 1478
AclWI GGATC 6 cut(s) 1012, 1122, 1132, 1231, 1490, 1503
AcoI YGGCCR 4 cut(s) 506, 723, 1073, 1678
AcsI RAATTY 4 cut(s) 7, 686, 923, 1875
AcuI CTGAAG 1 cut(s) 533
AdeI CACNNNGTG 1 cut(s) 809
AfaI GTAC 9 cut(s) 266, 719, 1024, 1079, 1149, 1355, 1526, 1559, 1911
AfiI CCNNNNNNNGG 3 cut(s) 785, 1433, 1985
AgeI ACCGGT 2 cut(s) 781, 1433
AhlI ACTAGT 1 cut(s) 1144
AjnI CCWGG 4 cut(s) 184, 508, 1336, 1384
AluBI AGCT 6 cut(s) 160, 210, 547, 653, 1001, 1475
AluI AGCT 6 cut(s) 160, 210, 547, 653, 1001, 1475
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 2 cut(s) 290, 665
AlwI GGATC 6 cut(s) 1012, 1122, 1132, 1231, 1490, 1503
Ama87I CYCGRG 1 cut(s) 289
Aor13HI TCCGGA 1 cut(s) 1492
AoxI GGCC 8 cut(s) 353, 506, 632, 723, 1073, 1135, 1678, 1758
ApeKI GCWGC 6 cut(s) 68, 451, 986, 1205, 1475, 1848
ApoI RAATTY 4 cut(s) 7, 686, 923, 1875
AsiGI ACCGGT 2 cut(s) 781, 1433
Asp700I GAANNNNTTC 2 cut(s) 654, 1170
AspLEI GCGC 3 cut(s) 106, 495, 577
AspS9I GGNCC 7 cut(s) 830, 844, 863, 959, 1135, 1436, 2018
AsuC2I CCSGG 6 cut(s) 290, 291, 466, 1212, 1308, 1737
AsuHPI GGTGA 8 cut(s) 72, 373, 436, 768, 841, 947, 1669, 1968
AsuNHI GCTAGC 1 cut(s) 1622
AvaI CYCGRG 1 cut(s) 289
AvaII GGWCC 6 cut(s) 830, 844, 863, 959, 1436, 2018
BalI TGGCCA 1 cut(s) 508
BamHI GGATCC 1 cut(s) 1495
BarI GAAGNNNNNNTAC 2 cut(s) 642, 674
BbsI GAAGAC 1 cut(s) 1163
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 6 cut(s) 55, 463, 998, 1192, 1462, 1860
BccI CCATC 3 cut(s) 121, 491, 1961
BceAI ACGGC 1 cut(s) 1060
BciT130I CCWGG 4 cut(s) 186, 510, 1338, 1386
BciVI GTATCC 1 cut(s) 1241
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 6 cut(s) 290, 291, 466, 1212, 1308, 1737
BcoDI GTCTC 2 cut(s) 290, 665
BcuI ACTAGT 1 cut(s) 1144
BfaI CTAG 6 cut(s) 588, 1145, 1407, 1623, 1662, 2012
BfmI CTRYAG 1 cut(s) 1860
BfoI RGCGCY 2 cut(s) 496, 578
BfuI GTATCC 1 cut(s) 1241
BisI GCNGC 7 cut(s) 69, 72, 452, 987, 1206, 1476, 1849
BlsI GCNGC 7 cut(s) 70, 73, 453, 988, 1207, 1477, 1850
BmcAI AGTACT 1 cut(s) 719
Bme18I GGWCC 6 cut(s) 830, 844, 863, 959, 1436, 2018
BmeT110I CYCGRG 1 cut(s) 289
BmgT120I GGNCC 7 cut(s) 830, 844, 863, 959, 1135, 1436, 2018
BmiI GGNNCC 1 cut(s) 1497
BmrI ACTGGG 3 cut(s) 277, 888, 1845
BmsI GCATC 3 cut(s) 100, 1337, 1775
BmtI GCTAGC 1 cut(s) 1626
BmuI ACTGGG 3 cut(s) 277, 888, 1845
BpiI GAAGAC 1 cut(s) 1163
BpmI CTGGAG 2 cut(s) 386, 1080
Bpu10I CCTNAGC 1 cut(s) 1653
BpuEI CTTGAG 1 cut(s) 1703
BpuMI CCSGG 6 cut(s) 290, 291, 466, 1212, 1308, 1737
Bsa29I ATCGAT 1 cut(s) 1156
BsaAI YACGTR 2 cut(s) 1195, 1698
BsaBI GATNNNNATC 2 cut(s) 711, 1500
BsaJI CCNNGG 6 cut(s) 289, 726, 1175, 1337, 1761, 1984
BsaWI WCCGGW 4 cut(s) 421, 781, 1433, 1492
Bsc4I CCNNNNNNNGG 3 cut(s) 785, 1433, 1985
Bse118I RCCGGY 3 cut(s) 781, 1433, 1680
Bse1I ACTGG 7 cut(s) 105, 272, 883, 1138, 1761, 1840, 1895
Bse8I GATNNNNATC 2 cut(s) 711, 1500
BseAI TCCGGA 1 cut(s) 1492
BseBI CCWGG 4 cut(s) 186, 510, 1338, 1386
BseCI ATCGAT 1 cut(s) 1156
BseDI CCNNGG 6 cut(s) 289, 726, 1175, 1337, 1761, 1984
BseGI GGATG 4 cut(s) 199, 277, 709, 801
BseJI GATNNNNATC 2 cut(s) 711, 1500
BseLI CCNNNNNNNGG 3 cut(s) 785, 1433, 1985
BseMII CTCAG 5 cut(s) 804, 1011, 1667, 1970, 2016
BseNI ACTGG 7 cut(s) 105, 272, 883, 1138, 1761, 1840, 1895
BseX3I CGGCCG 1 cut(s) 723
BseXI GCAGC 6 cut(s) 55, 463, 998, 1192, 1462, 1860
BsgI GTGCAG 1 cut(s) 1191
Bsh1236I CGCG 2 cut(s) 627, 1929
Bsh1285I CGRYCG 2 cut(s) 117, 726
BshFI GGCC 8 cut(s) 355, 508, 634, 725, 1075, 1137, 1680, 1760
BshTI ACCGGT 2 cut(s) 781, 1433
BshVI ATCGAT 1 cut(s) 1156
BsiEI CGRYCG 2 cut(s) 117, 726
BsiHKAI GWGCWC 1 cut(s) 625
BsiHKCI CYCGRG 1 cut(s) 289
BslFI GGGAC 2 cut(s) 43, 530
BslI CCNNNNNNNGG 3 cut(s) 785, 1433, 1985
BsmAI GTCTC 2 cut(s) 290, 665
BsmBI CGTCTC 2 cut(s) 290, 665
BsmFI GGGAC 2 cut(s) 43, 530
BsmI GAATGC 3 cut(s) 641, 1458, 1816
BsnI GGCC 8 cut(s) 355, 508, 634, 725, 1075, 1137, 1680, 1760
BsoBI CYCGRG 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 625
Bsp13I TCCGGA 1 cut(s) 1492
Bsp1407I TGTACA 1 cut(s) 1524
BspACI CCGC 5 cut(s) 71, 113, 406, 627, 1478
BspANI GGCC 8 cut(s) 355, 508, 634, 725, 1075, 1137, 1680, 1760
BspCNI CTCAG 5 cut(s) 805, 1010, 1666, 1969, 2017
BspDI ATCGAT 1 cut(s) 1156
BspEI TCCGGA 1 cut(s) 1492
BspFNI CGCG 2 cut(s) 627, 1929
BspHI TCATGA 1 cut(s) 1512
BspLI GGNNCC 1 cut(s) 1497
BspOI GCTAGC 1 cut(s) 1626
BspPI GGATC 6 cut(s) 1012, 1122, 1132, 1231, 1490, 1503
BsrFI RCCGGY 3 cut(s) 781, 1433, 1680
BsrGI TGTACA 1 cut(s) 1524
BsrI ACTGG 7 cut(s) 105, 272, 883, 1138, 1761, 1840, 1895
BssAI RCCGGY 3 cut(s) 781, 1433, 1680
BssECI CCNNGG 6 cut(s) 289, 726, 1175, 1337, 1761, 1984
BssT1I CCWWGG 2 cut(s) 1175, 1761
Bst2UI CCWGG 4 cut(s) 186, 510, 1338, 1386
Bst4CI ACNGT 5 cut(s) 418, 529, 1613, 1634, 1710
BstAUI TGTACA 1 cut(s) 1524
BstBAI YACGTR 2 cut(s) 1195, 1698
BstC8I GCNNGC 3 cut(s) 555, 625, 1624
BstDEI CTNAG 5 cut(s) 813, 997, 1653, 1956, 2025
BstDSI CCRYGG 1 cut(s) 1984
BstF5I GGATG 4 cut(s) 199, 277, 709, 801
BstFNI CGCG 2 cut(s) 627, 1929
BstH2I RGCGCY 2 cut(s) 496, 578
BstHHI GCGC 3 cut(s) 106, 495, 577
BstMAI GTCTC 2 cut(s) 290, 665
BstMCI CGRYCG 2 cut(s) 117, 726
BstMWI GCNNNNNNNGC 3 cut(s) 110, 1778, 1935
BstNI CCWGG 4 cut(s) 186, 510, 1338, 1386
BstSFI CTRYAG 1 cut(s) 1860
BstSNI TACGTA 1 cut(s) 1195
BstUI CGCG 2 cut(s) 627, 1929
BstV1I GCAGC 6 cut(s) 55, 463, 998, 1192, 1462, 1860
BstV2I GAAGAC 1 cut(s) 1163
BstX2I RGATCY 1 cut(s) 1495
BstYI RGATCY 1 cut(s) 1495
BstZI CGGCCG 1 cut(s) 723
Bsu15I ATCGAT 1 cut(s) 1156
BsuI GTATCC 1 cut(s) 1241
BsuRI GGCC 8 cut(s) 355, 508, 634, 725, 1075, 1137, 1680, 1760
BsuTUI ATCGAT 1 cut(s) 1156
BtgI CCRYGG 1 cut(s) 1984
BtsCI GGATG 4 cut(s) 199, 277, 709, 801
Cac8I GCNNGC 3 cut(s) 555, 625, 1624
CciI TCATGA 1 cut(s) 1512
CfoI GCGC 3 cut(s) 106, 495, 577
Cfr10I RCCGGY 3 cut(s) 781, 1433, 1680
Cfr13I GGNCC 7 cut(s) 830, 844, 863, 959, 1135, 1436, 2018
Cfr9I CCCGGG 1 cut(s) 289
ClaI ATCGAT 1 cut(s) 1156
Csp6I GTAC 9 cut(s) 265, 718, 1023, 1078, 1148, 1354, 1525, 1558, 1910
CspAI ACCGGT 2 cut(s) 781, 1433
CviAII CATG 9 cut(s) 65, 520, 601, 749, 1151, 1227, 1513, 1880, 1993
CviQI GTAC 9 cut(s) 265, 718, 1023, 1078, 1148, 1354, 1525, 1558, 1910
DdeI CTNAG 5 cut(s) 813, 997, 1653, 1956, 2025
DraI TTTAAA 1 cut(s) 337
DraIII CACNNNGTG 1 cut(s) 809
EaeI YGGCCR 4 cut(s) 506, 723, 1073, 1678
EagI CGGCCG 1 cut(s) 723
EclXI CGGCCG 1 cut(s) 723
Eco105I TACGTA 1 cut(s) 1195
Eco130I CCWWGG 2 cut(s) 1175, 1761
Eco32I GATATC 1 cut(s) 739
Eco47I GGWCC 6 cut(s) 830, 844, 863, 959, 1436, 2018
Eco52I CGGCCG 1 cut(s) 723
Eco57I CTGAAG 1 cut(s) 533
Eco88I CYCGRG 1 cut(s) 289
EcoO109I RGGNCCY 2 cut(s) 844, 2018
EcoRI GAATTC 2 cut(s) 7, 1875
EcoRII CCWGG 4 cut(s) 184, 508, 1336, 1384
EcoRV GATATC 1 cut(s) 739
EcoT14I CCWWGG 2 cut(s) 1175, 1761
EcoT22I ATGCAT 1 cut(s) 1330
ErhI CCWWGG 2 cut(s) 1175, 1761
Esp3I CGTCTC 2 cut(s) 290, 665
FaeI CATG 9 cut(s) 68, 523, 604, 752, 1154, 1230, 1516, 1883, 1996
FalI AAGNNNNNCTT 2 cut(s) 1174, 1206
FaqI GGGAC 2 cut(s) 43, 530
FatI CATG 9 cut(s) 64, 519, 600, 748, 1150, 1226, 1512, 1879, 1992
FauI CCCGC 1 cut(s) 399
FbaI TGATCA 1 cut(s) 798
Fnu4HI GCNGC 7 cut(s) 69, 72, 452, 987, 1206, 1476, 1849
FokI GGATG 4 cut(s) 206, 284, 716, 808
Fsp4HI GCNGC 7 cut(s) 69, 72, 452, 987, 1206, 1476, 1849
FspBI CTAG 6 cut(s) 588, 1145, 1407, 1623, 1662, 2012
GlaI GCGC 3 cut(s) 105, 494, 576
GluI GCNGC 7 cut(s) 69, 72, 452, 987, 1206, 1476, 1849
GsuI CTGGAG 2 cut(s) 386, 1080
HaeII RGCGCY 2 cut(s) 496, 578
HaeIII GGCC 8 cut(s) 355, 508, 634, 725, 1075, 1137, 1680, 1760
HhaI GCGC 3 cut(s) 106, 495, 577
Hin1II CATG 9 cut(s) 68, 523, 604, 752, 1154, 1230, 1516, 1883, 1996
Hin6I GCGC 3 cut(s) 104, 493, 575
HinP1I GCGC 3 cut(s) 104, 493, 575
HincII GTYRAC 2 cut(s) 433, 1801
HindII GTYRAC 2 cut(s) 433, 1801
HindIII AAGCTT 2 cut(s) 158, 545
HinfI GANTC 5 cut(s) 22, 441, 1261, 1534, 1989
HpaI GTTAAC 1 cut(s) 1801
HphI GGTGA 8 cut(s) 72, 373, 436, 768, 841, 947, 1669, 1968
Hpy166II GTNNAC 4 cut(s) 80, 433, 1025, 1801
Hpy188I TCNGA 5 cut(s) 583, 959, 1114, 1428, 2026
Hpy188III TCNNGA 9 cut(s) 118, 124, 683, 1097, 1463, 1493, 1513, 1720, 1962
Hpy8I GTNNAC 4 cut(s) 80, 433, 1025, 1801
HpyAV CCTTC 8 cut(s) 245, 494, 686, 1020, 1116, 1925, 2009, 2031
HpyCH4III ACNGT 5 cut(s) 418, 529, 1613, 1634, 1710
HpyCH4IV ACGT 6 cut(s) 32, 460, 858, 1120, 1194, 1697
HpyCH4V TGCA 7 cut(s) 451, 641, 748, 1208, 1328, 1781, 1789
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 1778, 1935
HpyF3I CTNAG 5 cut(s) 813, 997, 1653, 1956, 2025
HpySE526I ACGT 6 cut(s) 32, 460, 858, 1120, 1194, 1697
Hsp92II CATG 9 cut(s) 68, 523, 604, 752, 1154, 1230, 1516, 1883, 1996
HspAI GCGC 3 cut(s) 104, 493, 575
Kpn2I TCCGGA 1 cut(s) 1492
Ksp22I TGATCA 1 cut(s) 798
KspAI GTTAAC 1 cut(s) 1801
Lsp1109I GCAGC 6 cut(s) 55, 463, 998, 1192, 1462, 1860
LweI GCATC 3 cut(s) 100, 1337, 1775
MaeI CTAG 6 cut(s) 588, 1145, 1407, 1623, 1662, 2012
MaeII ACGT 6 cut(s) 32, 460, 858, 1120, 1194, 1697
MaeIII GTNAC 6 cut(s) 412, 1140, 1310, 1569, 1586, 1657
MboII GAAGA 5 cut(s) 450, 478, 1070, 1101, 1163
MfeI CAATTG 2 cut(s) 1377, 1400
MflI RGATCY 1 cut(s) 1495
MhlI GDGCHC 1 cut(s) 625
MlsI TGGCCA 1 cut(s) 508
MluNI TGGCCA 1 cut(s) 508
MlyI GAGTC 3 cut(s) 1255, 1543, 1998
MmeI TCCRAC 8 cut(s) 129, 734, 841, 1201, 1327, 1521, 1529, 1852
Mox20I TGGCCA 1 cut(s) 508
Mph1103I ATGCAT 1 cut(s) 1330
MroI TCCGGA 1 cut(s) 1492
MroXI GAANNNNTTC 2 cut(s) 654, 1170
MscI TGGCCA 1 cut(s) 508
MseI TTAA 4 cut(s) 240, 336, 978, 1800
MslI CAYNNNNRTG 2 cut(s) 897, 1578
Msp20I TGGCCA 1 cut(s) 508
MspA1I CMGCKG 2 cut(s) 406, 1475
MunI CAATTG 2 cut(s) 1377, 1400
Mva1269I GAATGC 3 cut(s) 641, 1458, 1816
MvaI CCWGG 4 cut(s) 186, 510, 1338, 1386
MvnI CGCG 2 cut(s) 627, 1929
MwoI GCNNNNNNNGC 3 cut(s) 110, 1778, 1935
NciI CCSGG 6 cut(s) 290, 291, 466, 1212, 1308, 1737
NheI GCTAGC 1 cut(s) 1622
NlaIII CATG 9 cut(s) 68, 523, 604, 752, 1154, 1230, 1516, 1883, 1996
NlaIV GGNNCC 1 cut(s) 1497
NmeAIII GCCGAG 3 cut(s) 701, 751, 962
NmuCI GTSAC 3 cut(s) 412, 1569, 1657
NsiI ATGCAT 1 cut(s) 1330
PagI TCATGA 1 cut(s) 1512
PctI GAATGC 3 cut(s) 641, 1458, 1816
PdmI GAANNNNTTC 2 cut(s) 654, 1170
PfeI GAWTC 2 cut(s) 22, 441
PfoI TCCNGGA 1 cut(s) 1735
PinAI ACCGGT 2 cut(s) 781, 1433
PkrI GCNGC 7 cut(s) 70, 73, 453, 988, 1207, 1477, 1850
PleI GAGTC 3 cut(s) 1255, 1542, 1997
PpsI GAGTC 3 cut(s) 1255, 1542, 1997
Ppu21I YACGTR 2 cut(s) 1195, 1698
PpuMI RGGWCCY 2 cut(s) 844, 2018
Psp5II RGGWCCY 2 cut(s) 844, 2018
Psp6I CCWGG 4 cut(s) 184, 508, 1336, 1384
PspGI CCWGG 4 cut(s) 184, 508, 1336, 1384
PspN4I GGNNCC 1 cut(s) 1497
PspPI GGNCC 7 cut(s) 830, 844, 863, 959, 1135, 1436, 2018
PspPPI RGGWCCY 2 cut(s) 844, 2018
PsrI GAACNNNNNNTAC 4 cut(s) 1423, 1455, 1508, 1540
PsuI RGATCY 1 cut(s) 1495
PvuII CAGCTG 1 cut(s) 1475
RsaI GTAC 9 cut(s) 266, 719, 1024, 1079, 1149, 1355, 1526, 1559, 1911
RsaNI GTAC 9 cut(s) 265, 718, 1023, 1078, 1148, 1354, 1525, 1558, 1910
RseI CAYNNNNRTG 2 cut(s) 897, 1578
SaqAI TTAA 4 cut(s) 240, 336, 978, 1800
SatI GCNGC 7 cut(s) 69, 72, 452, 987, 1206, 1476, 1849
Sau96I GGNCC 7 cut(s) 830, 844, 863, 959, 1135, 1436, 2018
ScaI AGTACT 1 cut(s) 719
SchI GAGTC 3 cut(s) 1255, 1543, 1998
SduI GDGCHC 1 cut(s) 625
SfaNI GCATC 3 cut(s) 100, 1337, 1775
SfcI CTRYAG 1 cut(s) 1860
SgrAI CRCCGGYG 1 cut(s) 781
SinI GGWCC 6 cut(s) 830, 844, 863, 959, 1436, 2018
SmaI CCCGGG 1 cut(s) 291
SmiMI CAYNNNNRTG 2 cut(s) 897, 1578
SmlI CTYRAG 1 cut(s) 1718
SmoI CTYRAG 1 cut(s) 1718
SnaBI TACGTA 1 cut(s) 1195
SpeI ACTAGT 1 cut(s) 1144
SsiI CCGC 5 cut(s) 71, 113, 406, 627, 1478
SspI AATATT 1 cut(s) 1300
SspMI CTAG 6 cut(s) 588, 1145, 1407, 1623, 1662, 2012
StyI CCWWGG 2 cut(s) 1175, 1761
TaaI ACNGT 5 cut(s) 418, 529, 1613, 1634, 1710
TaiI ACGT 6 cut(s) 35, 463, 861, 1123, 1197, 1700
TaqI TCGA 6 cut(s) 94, 117, 489, 565, 1156, 1832
TaqII GACCGA 1 cut(s) 633
TatI WGTACW 4 cut(s) 717, 1147, 1353, 1524
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 2 cut(s) 22, 441
Tru1I TTAA 4 cut(s) 240, 336, 978, 1800
Tru9I TTAA 4 cut(s) 240, 336, 978, 1800
TseFI GTSAC 3 cut(s) 412, 1569, 1657
TseI GCWGC 6 cut(s) 68, 451, 986, 1205, 1475, 1848
Tsp45I GTSAC 3 cut(s) 412, 1569, 1657
TspDTI ATGAA 9 cut(s) 53, 367, 433, 589, 1075, 1179, 1287, 1529, 1868
TspGWI ACGGA 4 cut(s) 314, 362, 1010, 2001
TspMI CCCGGG 1 cut(s) 289
VpaK11BI GGWCC 6 cut(s) 830, 844, 863, 959, 1436, 2018
XapI RAATTY 4 cut(s) 7, 686, 923, 1875
XmaI CCCGGG 1 cut(s) 289
XmnI GAANNNNTTC 2 cut(s) 654, 1170
XspI CTAG 6 cut(s) 588, 1145, 1407, 1623, 1662, 2012
ZrmI AGTACT 1 cut(s) 719
Zsp2I ATGCAT 1 cut(s) 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.