MD01G1043200.v1.1

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000523
Physical Location & Seq
Reverse (-)
14185165 .. 14188981
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1043200.v1.1.491

Sequence Viewer

Length: 1776 bp
ATGGCATTGTCCGACTCAATTTTTCTTATCCCGGAGGAGACGTACTGGGACGTTGTCTGGAACAGAGATGGAGGTAAAGATGTGGTGGACAAGCTACAAGGAACTGGATTTAGAGTCATTACAGAGAGGGCCGACCAAATAGAGATTTCTTTTACTAAAACATATAATGTTTCCCTTGGTAATGCTACACTTCCCATGAATGTTGACAAAAGATACATAGTGCAACGTGGTCGTGATGGGTTTTATGCCTACGCAATATTCGAGCGTCTTAAGGGGTGGCCCAGAACTAATGTGGATCAAATTAGATTTGTGTACAAACTTCAACAAGACAAATTTCCATTCATGGCTATATCAGACAACAGAAAAAGAGTCATGCCAACAGCCAGAGACCGTGCAAATGGCAAGCCCCTTGCCTACCCTGAAGCTGTCCTTTTAACCAAAACAAGCAATCCTGACCTTCGAGGAGAGGTAGATGACAAGTACCAATACTCAACTGAGGACAAAGATAACAAAGTTCATAGTTGGATTTGTAATGAACCGGCCGTAGGGTTTTGGATAATTACATCAAGTGATGAGTTTCGTACAGCCGGACCTTTCAAACAAGACCTTACCTCTCATGCGGGTCCAACTGTGCTCTCTATGTTTGCTAGTACTCATTATGGTGGGAAGGAAGTTGGGATGACATTCAAAGATGGGGAGGCCTGGAATAAGGTTTTTGGGCCTGTCTTTGTCTATCTTAATTCAGTTCGAAGCTCAAATAATTCCCTCAGACTCTGGGAAAATGCTAAAGAACAGATGCTTGAAGAAGTCAAAAGATGGCCTTACAATTTCACTCAATCTGAAGATTTTCCATCATCGGATAAACGTGGATCAGTTGCGGGCCAGTTACTAATACAAGATCAGTACATCAACGAGAGCCTTGTATGGGCAAGTTCCGCTTACGTGGGATTGGCAGCACCTGGAAATGTGGGATCATGGCAAAAGGAAAGCAAGGGTTATCAATTTTGGACTCAAACTAACAAGCAAGGTTATTTCCTCATTAAAGACGTTCGACCTGGGAACTATAGTTTGTATGCAACAGTTCCCGGCGTTGTTGGGGATTACAAATATGAAACTATTATTAAAATTAAACCAAGACGTGGAATTAATCTGGGCAATATAACTTACGTACCTCCAAGAAATGGTCCGACCCTGTGGGAAATTGGCATCCCAGATCGATCAGCTGCCGAGTTCAATGTACCCGATCCGTCCCCAACTCTTAGGAACCAATTATACACCAATCACACGGACAAGTTTAGGCAATACGGCTTGTGGGATCGCTATACAGATTTATACCCTAACCATGATCTCATCTACACCGTTGGTGCTGACGATTACCATGACAAGTGGTTTTTTGCTCATGTGACAAGGAACACTGGAAATAATACGTATGAAGCAACCACATGGCGAATTCTATTTCAACTTAAGAATTTGACTACTACCGGAAATTATACACTCCAATTGGCATTGGCCTCAGCCAACAATGCAGAAGTACAGGTTCGATTTAACAACCAGAGCGCCAAACCACCTCACTTTACGACAGGACTAATAGGGAAGGACAATGCCATAGCAAGACATGGAATTCATGGTTTGTACTGGTTGTTCAGTATTATTGTACCAAGTTATCGACTACAAGAAGGAAACAACAGCATCTTTCTTACACAGTCAAGAAGATTGGGCTCCTTCGACGGCGTCATGTATGACTATATTCGACTAGAAGGACCTCCTCAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

592

Amino Acids

67.68

Weight (kDa)

8.64

Isoelectric Point (pI)

32.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 13 - 157 4.8e-58 Rhamnogalacturonate lyase family
fn3_3 PF14686 311 - 382 3.7e-21 Polysaccharide lyase family 4, domain II
CBM-like PF14683 397 - 585 1.4e-47 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1139, 1181
AciI CCGC 3 cut(s) 620, 878, 936
AclWI GGATC 5 cut(s) 303, 877, 979, 1238, 1323
AcoI YGGCCR 1 cut(s) 540
AcsI RAATTY 4 cut(s) 332, 1449, 1468, 1620
AcuI CTGAAG 2 cut(s) 441, 861
AcyI GRCGYC 1 cut(s) 1731
AfiI CCNNNNNNNGG 4 cut(s) 545, 925, 1139, 1181
AflII CTTAAG 2 cut(s) 269, 1463
AgsI TTSAA 6 cut(s) 323, 598, 688, 803, 1234, 1460
AjiI CACGTC 1 cut(s) 1139
AjnI CCWGG 3 cut(s) 701, 958, 1054
AluBI AGCT 4 cut(s) 94, 425, 753, 1223
AluI AGCT 4 cut(s) 94, 425, 753, 1223
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 2 cut(s) 32, 381
AlwI GGATC 5 cut(s) 303, 877, 979, 1238, 1323
AlwNI CAGNNNCTG 2 cut(s) 774, 959
AoxI GGCC 8 cut(s) 129, 278, 540, 699, 719, 818, 880, 1509
ApeKI GCWGC 2 cut(s) 953, 1223
ApoI RAATTY 4 cut(s) 332, 1449, 1468, 1620
AseI ATTAAT 1 cut(s) 1146
Asp700I GAANNNNTTC 1 cut(s) 846
AspLEI GCGC 1 cut(s) 1559
AspS9I GGNCC 8 cut(s) 129, 279, 590, 623, 719, 880, 1184, 1760
AsuC2I CCSGG 2 cut(s) 32, 1086
AsuII TTCGAA 1 cut(s) 748
AvaII GGWCC 4 cut(s) 590, 623, 1184, 1760
BaeI ACNNNNGTAYC 2 cut(s) 1152, 1185
BanII GRGCYC 1 cut(s) 1721
Bbv12I GWGCWC 1 cut(s) 636
BbvCI CCTCAGC 1 cut(s) 1513
BbvI GCAGC 2 cut(s) 965, 1210
BccI CCATC 5 cut(s) 62, 230, 686, 810, 859
BceAI ACGGC 3 cut(s) 527, 1321, 1744
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BciT130I CCWGG 3 cut(s) 703, 960, 1056
BcnI CCSGG 2 cut(s) 32, 1086
BcoDI GTCTC 2 cut(s) 32, 381
BfaI CTAG 2 cut(s) 648, 1754
BfmI CTRYAG 1 cut(s) 1063
BfoI RGCGCY 1 cut(s) 1560
BfrI CTTAAG 2 cut(s) 269, 1463
BisI GCNGC 2 cut(s) 954, 1224
BlsI GCNGC 2 cut(s) 955, 1225
BmcAI AGTACT 1 cut(s) 652
Bme1390I CCNGG 5 cut(s) 32, 703, 960, 1056, 1086
Bme18I GGWCC 4 cut(s) 590, 623, 1184, 1760
BmgBI CACGTC 1 cut(s) 1139
BmgT120I GGNCC 8 cut(s) 129, 279, 590, 623, 719, 880, 1184, 1760
BmiI GGNNCC 3 cut(s) 624, 1265, 1720
BmrFI CCNGG 5 cut(s) 32, 703, 960, 1056, 1086
BmrI ACTGGG 1 cut(s) 55
BmsI GCATC 3 cut(s) 786, 1215, 1698
BmuI ACTGGG 1 cut(s) 55
Bpu10I CCTNAGC 1 cut(s) 1513
Bpu14I TTCGAA 1 cut(s) 748
BpuMI CCSGG 2 cut(s) 32, 1086
Bsa29I ATCGAT 1 cut(s) 1216
BsaAI YACGTR 3 cut(s) 943, 1168, 1428
BsaHI GRCGYC 1 cut(s) 1731
BsaI GGTCTC 1 cut(s) 381
BsaJI CCNNGG 2 cut(s) 175, 1055
BsaWI WCCGGW 1 cut(s) 1481
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 4 cut(s) 545, 925, 1139, 1181
Bse118I RCCGGY 1 cut(s) 538
Bse1I ACTGG 5 cut(s) 50, 109, 883, 1420, 1640
BseBI CCWGG 3 cut(s) 703, 960, 1056
BseCI ATCGAT 1 cut(s) 1216
BseDI CCNNGG 2 cut(s) 175, 1055
BseGI GGATG 2 cut(s) 684, 1206
BseLI CCNNNNNNNGG 4 cut(s) 545, 925, 1139, 1181
BseMII CTCAG 3 cut(s) 486, 781, 1527
BseNI ACTGG 5 cut(s) 50, 109, 883, 1420, 1640
BseRI GAGGAG 3 cut(s) 50, 477, 1755
BseX3I CGGCCG 1 cut(s) 540
BseXI GCAGC 2 cut(s) 965, 1210
Bsh1285I CGRYCG 1 cut(s) 543
BshFI GGCC 8 cut(s) 131, 280, 542, 701, 721, 820, 882, 1511
BshVI ATCGAT 1 cut(s) 1216
BsiEI CGRYCG 1 cut(s) 543
BsiHKAI GWGCWC 1 cut(s) 636
BsiSI CCGG 5 cut(s) 32, 539, 588, 1086, 1482
BslFI GGGAC 2 cut(s) 62, 1234
BslI CCNNNNNNNGG 4 cut(s) 545, 925, 1139, 1181
BsmAI GTCTC 2 cut(s) 32, 381
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 62, 1234
BsnI GGCC 8 cut(s) 131, 280, 542, 701, 721, 820, 882, 1511
Bso31I GGTCTC 1 cut(s) 381
Bsp119I TTCGAA 1 cut(s) 748
Bsp1286I GDGCHC 2 cut(s) 636, 1721
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 9 cut(s) 295, 869, 898, 971, 1213, 1217, 1243, 1315, 1345
BspACI CCGC 3 cut(s) 620, 878, 936
BspANI GGCC 8 cut(s) 131, 280, 542, 701, 721, 820, 882, 1511
BspCNI CTCAG 3 cut(s) 487, 780, 1526
BspDI ATCGAT 1 cut(s) 1216
BspLI GGNNCC 3 cut(s) 624, 1265, 1720
BspPI GGATC 5 cut(s) 303, 877, 979, 1238, 1323
BspT104I TTCGAA 1 cut(s) 748
BspTI CTTAAG 2 cut(s) 269, 1463
BspTNI GGTCTC 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 538
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 5 cut(s) 50, 109, 883, 1420, 1640
BssAI RCCGGY 1 cut(s) 538
BssECI CCNNGG 2 cut(s) 175, 1055
BssMI GATC 9 cut(s) 295, 869, 898, 971, 1213, 1217, 1243, 1315, 1345
BssNI GRCGYC 1 cut(s) 1731
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 3 cut(s) 703, 960, 1056
Bst4CI ACNGT 5 cut(s) 392, 631, 1081, 1360, 1704
BstACI GRCGYC 1 cut(s) 1731
BstAFI CTTAAG 2 cut(s) 269, 1463
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 3 cut(s) 943, 1168, 1428
BstBI TTCGAA 1 cut(s) 748
BstC8I GCNNGC 2 cut(s) 404, 880
BstDEI CTNAG 4 cut(s) 495, 767, 1259, 1513
BstF5I GGATG 2 cut(s) 684, 1206
BstH2I RGCGCY 1 cut(s) 1560
BstHHI GCGC 1 cut(s) 1559
BstKTI GATC 9 cut(s) 298, 872, 901, 974, 1216, 1220, 1246, 1318, 1348
BstMAI GTCTC 2 cut(s) 32, 381
BstMBI GATC 9 cut(s) 295, 869, 898, 971, 1213, 1217, 1243, 1315, 1345
BstMCI CGRYCG 1 cut(s) 543
BstMWI GCNNNNNNNGC 2 cut(s) 935, 1523
BstNI CCWGG 3 cut(s) 703, 960, 1056
BstSCI CCNGG 5 cut(s) 30, 701, 958, 1054, 1084
BstSFI CTRYAG 1 cut(s) 1063
BstSNI TACGTA 2 cut(s) 1168, 1428
BstV1I GCAGC 2 cut(s) 965, 1210
BstZI CGGCCG 1 cut(s) 540
Bsu15I ATCGAT 1 cut(s) 1216
BsuRI GGCC 8 cut(s) 131, 280, 542, 701, 721, 820, 882, 1511
BsuTUI ATCGAT 1 cut(s) 1216
BtrI CACGTC 1 cut(s) 1139
BtsCI GGATG 2 cut(s) 684, 1206
BtsIMutI CAGTG 1 cut(s) 1413
Cac8I GCNNGC 2 cut(s) 404, 880
CaiI CAGNNNCTG 2 cut(s) 774, 959
CfoI GCGC 1 cut(s) 1559
Cfr10I RCCGGY 1 cut(s) 538
Cfr13I GGNCC 8 cut(s) 129, 279, 590, 623, 719, 880, 1184, 1760
ClaI ATCGAT 1 cut(s) 1216
CseI GACGC 2 cut(s) 254, 1720
DdeI CTNAG 4 cut(s) 495, 767, 1259, 1513
DpnI GATC 9 cut(s) 297, 871, 900, 973, 1215, 1219, 1245, 1317, 1347
DpnII GATC 9 cut(s) 295, 869, 898, 971, 1213, 1217, 1243, 1315, 1345
EaeI YGGCCR 1 cut(s) 540
EagI CGGCCG 1 cut(s) 540
EclXI CGGCCG 1 cut(s) 540
Eco105I TACGTA 2 cut(s) 1168, 1428
Eco130I CCWWGG 1 cut(s) 175
Eco147I AGGCCT 1 cut(s) 701
Eco24I GRGCYC 1 cut(s) 1721
Eco31I GGTCTC 1 cut(s) 381
Eco47I GGWCC 4 cut(s) 590, 623, 1184, 1760
Eco52I CGGCCG 1 cut(s) 540
Eco57I CTGAAG 2 cut(s) 441, 861
EcoO109I RGGNCCY 1 cut(s) 1760
EcoRI GAATTC 2 cut(s) 1449, 1620
EcoRII CCWGG 3 cut(s) 701, 958, 1054
EcoT14I CCWWGG 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 1721
ErhI CCWWGG 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 32
FalI AAGNNNNNCTT 6 cut(s) 414, 446, 805, 837, 922, 954
FaqI GGGAC 2 cut(s) 62, 1234
FauI CCCGC 2 cut(s) 613, 871
Fnu4HI GCNGC 2 cut(s) 954, 1224
FokI GGATG 2 cut(s) 691, 1193
FriOI GRGCYC 1 cut(s) 1721
Fsp4HI GCNGC 2 cut(s) 954, 1224
FspBI CTAG 2 cut(s) 648, 1754
GlaI GCGC 1 cut(s) 1558
GluI GCNGC 2 cut(s) 954, 1224
HaeII RGCGCY 1 cut(s) 1560
HaeIII GGCC 8 cut(s) 131, 280, 542, 701, 721, 820, 882, 1511
HapII CCGG 5 cut(s) 32, 539, 588, 1086, 1482
HgaI GACGC 2 cut(s) 254, 1720
HhaI GCGC 1 cut(s) 1559
Hin1I GRCGYC 1 cut(s) 1731
Hin6I GCGC 1 cut(s) 1557
HinP1I GCGC 1 cut(s) 1557
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 5 cut(s) 14, 114, 369, 771, 1009
HpaII CCGG 5 cut(s) 32, 539, 588, 1086, 1482
Hpy166II GTNNAC 3 cut(s) 88, 205, 313
Hpy188I TCNGA 6 cut(s) 13, 355, 770, 841, 859, 1188
Hpy188III TCNNGA 4 cut(s) 58, 233, 452, 1707
Hpy8I GTNNAC 3 cut(s) 88, 205, 313
Hpy99I CGWCG 1 cut(s) 1730
HpyAV CCTTC 6 cut(s) 467, 661, 1588, 1670, 1732, 1751
HpyCH4III ACNGT 5 cut(s) 392, 631, 1081, 1360, 1704
HpyCH4IV ACGT 9 cut(s) 41, 51, 226, 865, 942, 1047, 1138, 1167, 1427
HpyCH4V TGCA 4 cut(s) 223, 395, 1076, 1526
HpyF10VI GCNNNNNNNGC 2 cut(s) 935, 1523
HpyF3I CTNAG 4 cut(s) 495, 767, 1259, 1513
HpySE526I ACGT 9 cut(s) 41, 51, 226, 865, 942, 1047, 1138, 1167, 1427
Hsp92I GRCGYC 1 cut(s) 1731
HspAI GCGC 1 cut(s) 1557
Kzo9I GATC 9 cut(s) 295, 869, 898, 971, 1213, 1217, 1243, 1315, 1345
LmnI GCTCC 1 cut(s) 1724
Lsp1109I GCAGC 2 cut(s) 965, 1210
LweI GCATC 3 cut(s) 786, 1215, 1698
MaeI CTAG 2 cut(s) 648, 1754
MaeII ACGT 9 cut(s) 41, 51, 226, 865, 942, 1047, 1138, 1167, 1427
MaeIII GTNAC 2 cut(s) 885, 1402
MalI GATC 9 cut(s) 297, 871, 900, 973, 1215, 1219, 1245, 1317, 1347
MboI GATC 9 cut(s) 295, 869, 898, 971, 1213, 1217, 1243, 1315, 1345
MboII GAAGA 3 cut(s) 815, 854, 1722
MfeI CAATTG 1 cut(s) 1499
MhlI GDGCHC 2 cut(s) 636, 1721
MlyI GAGTC 5 cut(s) 8, 123, 378, 765, 1003
MmeI TCCRAC 4 cut(s) 36, 503, 650, 1211
MroXI GAANNNNTTC 1 cut(s) 846
MseI TTAA 9 cut(s) 270, 434, 738, 1041, 1122, 1128, 1146, 1464, 1545
MslI CAYNNNNRTG 1 cut(s) 660
MspA1I CMGCKG 1 cut(s) 1223
MspCI CTTAAG 2 cut(s) 269, 1463
MspI CCGG 5 cut(s) 32, 539, 588, 1086, 1482
MspR9I CCNGG 5 cut(s) 32, 703, 960, 1056, 1086
MunI CAATTG 1 cut(s) 1499
MvaI CCWGG 3 cut(s) 703, 960, 1056
MwoI GCNNNNNNNGC 2 cut(s) 935, 1523
NciI CCSGG 2 cut(s) 32, 1086
NdeII GATC 9 cut(s) 295, 869, 898, 971, 1213, 1217, 1243, 1315, 1345
NlaIV GGNNCC 3 cut(s) 624, 1265, 1720
NmeAIII GCCGAG 1 cut(s) 1252
NmuCI GTSAC 1 cut(s) 1402
NspV TTCGAA 1 cut(s) 748
PceI AGGCCT 1 cut(s) 701
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 846
PflFI GACNNNGTC 2 cut(s) 53, 1730
PflMI CCANNNNNTGG 2 cut(s) 1139, 1181
PfoI TCCNGGA 1 cut(s) 30
PkrI GCNGC 2 cut(s) 955, 1225
PleI GAGTC 5 cut(s) 8, 122, 377, 765, 1003
PpsI GAGTC 5 cut(s) 8, 122, 377, 765, 1003
Ppu21I YACGTR 3 cut(s) 943, 1168, 1428
PpuMI RGGWCCY 1 cut(s) 1760
PshBI ATTAAT 1 cut(s) 1146
Psp5II RGGWCCY 1 cut(s) 1760
Psp6I CCWGG 3 cut(s) 701, 958, 1054
PspGI CCWGG 3 cut(s) 701, 958, 1054
PspN4I GGNNCC 3 cut(s) 624, 1265, 1720
PspPI GGNCC 8 cut(s) 129, 279, 590, 623, 719, 880, 1184, 1760
PspPPI RGGWCCY 1 cut(s) 1760
PsrI GAACNNNNNNTAC 4 cut(s) 1256, 1288, 1625, 1657
PstNI CAGNNNCTG 2 cut(s) 774, 959
PsyI GACNNNGTC 2 cut(s) 53, 1730
PvuII CAGCTG 1 cut(s) 1223
RseI CAYNNNNRTG 1 cut(s) 660
SaqAI TTAA 9 cut(s) 270, 434, 738, 1041, 1122, 1128, 1146, 1464, 1545
SatI GCNGC 2 cut(s) 954, 1224
Sau3AI GATC 9 cut(s) 295, 869, 898, 971, 1213, 1217, 1243, 1315, 1345
Sau96I GGNCC 8 cut(s) 129, 279, 590, 623, 719, 880, 1184, 1760
ScaI AGTACT 1 cut(s) 652
SchI GAGTC 5 cut(s) 8, 123, 378, 765, 1003
ScrFI CCNGG 5 cut(s) 32, 703, 960, 1056, 1086
SduI GDGCHC 2 cut(s) 636, 1721
SfaNI GCATC 3 cut(s) 786, 1215, 1698
SfcI CTRYAG 1 cut(s) 1063
SfuI TTCGAA 1 cut(s) 748
SinI GGWCC 4 cut(s) 590, 623, 1184, 1760
SmiMI CAYNNNNRTG 1 cut(s) 660
SmlI CTYRAG 2 cut(s) 269, 1463
SmoI CTYRAG 2 cut(s) 269, 1463
SnaBI TACGTA 2 cut(s) 1168, 1428
SseBI AGGCCT 1 cut(s) 701
SsiI CCGC 3 cut(s) 620, 878, 936
SspI AATATT 1 cut(s) 258
SspMI CTAG 2 cut(s) 648, 1754
StuI AGGCCT 1 cut(s) 701
StyD4I CCNGG 5 cut(s) 30, 701, 958, 1054, 1084
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 5 cut(s) 392, 631, 1081, 1360, 1704
TaiI ACGT 9 cut(s) 44, 54, 229, 868, 945, 1050, 1141, 1170, 1430
TaqI TCGA 9 cut(s) 261, 460, 748, 1051, 1216, 1540, 1666, 1725, 1750
TatI WGTACW 5 cut(s) 312, 650, 903, 1531, 1632
Tru1I TTAA 9 cut(s) 270, 434, 738, 1041, 1122, 1128, 1146, 1464, 1545
Tru9I TTAA 9 cut(s) 270, 434, 738, 1041, 1122, 1128, 1146, 1464, 1545
TscAI CASTG 1 cut(s) 1420
TseFI GTSAC 1 cut(s) 1402
TseI GCWGC 2 cut(s) 953, 1223
Tsp45I GTSAC 1 cut(s) 1402
TspDTI ATGAA 7 cut(s) 212, 331, 506, 549, 1125, 1446, 1613
TspGWI ACGGA 2 cut(s) 1236, 1301
TspRI CASTG 1 cut(s) 1420
Tth111I GACNNNGTC 2 cut(s) 53, 1730
Van91I CCANNNNNTGG 2 cut(s) 1139, 1181
Vha464I CTTAAG 2 cut(s) 269, 1463
VpaK11BI GGWCC 4 cut(s) 590, 623, 1184, 1760
VspI ATTAAT 1 cut(s) 1146
XapI RAATTY 4 cut(s) 332, 1449, 1468, 1620
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 846
XspI CTAG 2 cut(s) 648, 1754
ZrmI AGTACT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.