Rh2AG219300

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000523
Physical Location & Seq
Forward (+)
22742983 .. 22748877
5895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG219300.1

Sequence Viewer

Length: 2061 bp
ATGGAGAAGATGAGGAAATGGGGTTGGCTTCTGGGGTCATCAGCAGCAATTCTTCTTCTGGTTTTGTTCTTGGCTGGTCATGGAACTTACGACGAGACGACGAGCCGAGTAAGACGATCACTGAGGAGCATAATTCCTCGGCGACAACAGGGGGTCAATTTACTAACAAGGGACCCCAAGAGGGTTGTTTTGGATAATGGCATTGTCAAAGTCATTTTCTCTAATCCGGGCGGATACGTTATTGGTATACAGTACAGAGGCCTTGACATACTTGAAATGGGGAATGAAGAAAATAATAGAGGGTACTGGGATGTGGTCTGGAAAAAGAATATAACTGACAAACTAGAAGCAACAAAATTTAGGGTTATTACCACGAGGCCAGACCAAGCAGAAATTTCCTTCCTAAGAACATATGATGTTTCCCTTGGTAACGGCACGAACACAATTCCCTTGACCGTAGACAAAAGGTACATACTGCAGCGGGGTGGTTCTGGTTTCTATGCATATGGAATATTCGAGCGCCTTGATGGGTGGCCGGCGGTACAGGATATGGATCAAATTAGAATGGTTTACAAGCTTGAACAAGCCAAATTTCATTATATGGCGTTATCAGACACCAGGCAAAGAAGCATGCCAACAGCAGCAGACCGTGGTCGTGGTCAGAAACTTGCGTATAAAGAAGCTTGTCTTTTGACTAATCCAAGCAATCCTGACTTCAGCGGAGAGGTAGATGACAAGTACCAGTACTCAATCGAGGACAAAGATAACAAAGTTCATGGTTGGATTAGTACTGATGAATCTGTGGGATTCTGGATGATCACACCCAGCGACGAATTTCGTACAGGAGGCCCCTTTAAGCAGGACCTCACCTCCCATGTTGGTCCAACCACCCTCTCCATGTTTGTTAGTACTCATTATGCTGGGAAGGAACTTCAGATCAAATTTGAAGAAGGTGAGGCATGGAAAAAGGTTTTTGGGCCTGTCTTTGTGTATCTTAATTCGGCTCCAAGCTCAAAGAATTCCATCCGTATACTTTGGAATGATGCTAAAAAGCAGATGCTTAAAGAAGTCAAGAACTGGCCGTATAATTTCACTCAATCCGAGGACTTTCCTTCTTCCCATCAACGGGGATCAGTTTTGGGCCAATTACTAATACATGATCGATACATCAATGAGAGTCTTTTCCCAGCAAGTTCTGCTTATGTGGGATTGGCTGCACCCGGAGAAGTGGGATCATGGCAAAGGGAAAACAAGGGTTATCAATTTTGGAATCAAGCTGACGAAGAAGGTAGATTCATTATCAAAAATGTTCGACCCGGAAACTATAGTTTTTATGCATGGGTTCCTGGCATTGTTGGGGATTACAAATATGAGGTTGATATTACAATTACTCCAGGGTCTAATATCAAATTGGACAATCTTACTTACGAACCTCCAAGAAATGGTCCTACTTTGTGGGAAATTGGCATCCCTGATCGCACTGCTGCCGAATTCTACGTACCAGATCCTTATCCAACTCTTTTGAACAATTTATACACCCAGAATCACACAGAAAATTTTAGGCAATACGGGTTGTGGGCACGTTATGGAGAGGGATACCCTCATAATGATCTTGTGTACACCGTAGCTATCAACAATTACCATGATGATTGGTTCTATGCTCAAGTGACCAGAAATACTGGAAATGAAACCTATGTAGGAACGACGTGGCAAATTCTATTTGAACTTCATAATGTCACAAATCCAGGAAATTATACACTCCAATTGGCATTGGCCTCAGCAAACAATGCAGAATTGCAGGTTCGAATTAACAACAAGAGCGATGAGCAACCTCATTTTTCATCAAAGTTAATTGGAGGGGACAATGCGATAGCAAGACATGGAATTCATGGTTTGTACTGGTTGTTTAGTGTTGAGATACCAAGCTCGCTACTAAAAGAAGGAATCAACACCGTCTATCTTACTCAAGCGAGAGGTGGTGAAACTTTCCAAGGAGTCATGTATGACTACATCAGACTAGAAGCACCTTCATCACACACTGATTCACAAGTAGTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

686

Amino Acids

78.21

Weight (kDa)

6.24

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 52 - 243 2.1e-72 Rhamnogalacturonate lyase family
fn3_3 PF14686 398 - 471 5e-25 Polysaccharide lyase family 4, domain II
CBM-like PF14683 484 - 673 1.1e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1789
AccB7I CCANNNNNTGG 1 cut(s) 1442
AccI GTMKAC 3 cut(s) 247, 459, 1030
AciI CCGC 4 cut(s) 231, 481, 539, 720
AclWI GGATC 4 cut(s) 561, 1138, 1240, 1499
AcoI YGGCCR 2 cut(s) 533, 1079
AcuI CTGAAG 2 cut(s) 700, 917
AfiI CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
AgsI TTSAA 5 cut(s) 275, 581, 947, 1525, 1724
AjiI CACGTC 1 cut(s) 1707
AjnI CCWGG 4 cut(s) 617, 1345, 1393, 1744
AluBI AGCT 6 cut(s) 577, 683, 1011, 1277, 1628, 1926
AluI AGCT 6 cut(s) 577, 683, 1011, 1277, 1628, 1926
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 4 cut(s) 561, 1138, 1240, 1499
AoxI GGCC 8 cut(s) 259, 377, 533, 847, 977, 1079, 1141, 1773
ApeKI GCWGC 5 cut(s) 44, 478, 641, 1214, 1484
AspLEI GCGC 1 cut(s) 522
AspS9I GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
AsuC2I CCSGG 3 cut(s) 228, 1221, 1317
AsuHPI GGTGA 3 cut(s) 859, 965, 1991
AsuII TTCGAA 1 cut(s) 1804
AvaII GGWCC 4 cut(s) 172, 862, 881, 1445
BaeGI GKGCMC 1 cut(s) 1582
BauI CACGAG 1 cut(s) 373
BbvCI CCTCAGC 1 cut(s) 1777
BbvI GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
BccI CCATC 3 cut(s) 521, 1031, 1128
BceAI ACGGC 2 cut(s) 448, 1066
BciT130I CCWGG 4 cut(s) 619, 1347, 1395, 1746
BciVI GTATCC 2 cut(s) 227, 1589
BclI TGATCA 1 cut(s) 816
BcnI CCSGG 3 cut(s) 228, 1221, 1317
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 2 cut(s) 344, 2018
BfmI CTRYAG 2 cut(s) 476, 1324
BfoI RGCGCY 1 cut(s) 523
BfuAI ACCTGC 1 cut(s) 1789
BfuI GTATCC 2 cut(s) 227, 1589
BisI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
BlsI GCNGC 5 cut(s) 46, 480, 643, 1216, 1486
BmcAI AGTACT 3 cut(s) 746, 790, 910
Bme1390I CCNGG 7 cut(s) 228, 619, 1221, 1317, 1347, 1395, 1746
Bme18I GGWCC 4 cut(s) 172, 862, 881, 1445
BmgBI CACGTC 1 cut(s) 1707
BmgT120I GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
BmiI GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
BmrFI CCNGG 7 cut(s) 228, 619, 1221, 1317, 1347, 1395, 1746
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 3 cut(s) 1033, 1047, 1476
BmuI ACTGGG 1 cut(s) 316
BoxI GACNNNNGTC 1 cut(s) 651
BpmI CTGGAG 1 cut(s) 1377
Bpu10I CCTNAGC 1 cut(s) 1777
Bpu14I TTCGAA 1 cut(s) 1804
BpuEI CTTGAG 2 cut(s) 1647, 1950
BpuMI CCSGG 3 cut(s) 228, 1221, 1317
Bsa29I ATCGAT 1 cut(s) 1162
BsaAI YACGTR 1 cut(s) 1498
BsaBI GATNNNNATC 2 cut(s) 552, 1509
BsaJI CCNNGG 6 cut(s) 137, 424, 649, 1101, 1394, 1990
BsaXI ACNNNNNCTCC 4 cut(s) 854, 884, 1375, 1405
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
Bse118I RCCGGY 1 cut(s) 535
Bse1I ACTGG 5 cut(s) 311, 742, 1082, 1684, 1904
Bse8I GATNNNNATC 2 cut(s) 552, 1509
BseBI CCWGG 4 cut(s) 619, 1347, 1395, 1746
BseCI ATCGAT 1 cut(s) 1162
BseDI CCNNGG 6 cut(s) 137, 424, 649, 1101, 1394, 1990
BseGI GGATG 4 cut(s) 316, 819, 1023, 1467
BseJI GATNNNNATC 2 cut(s) 552, 1509
BseLI CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
BseMII CTCAG 2 cut(s) 113, 1791
BseNI ACTGG 5 cut(s) 311, 742, 1082, 1684, 1904
BseRI GAGGAG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 1582
BseXI GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
BseYI CCCAGC 3 cut(s) 824, 920, 1186
BsgI GTGCAG 1 cut(s) 1200
BshFI GGCC 8 cut(s) 261, 379, 535, 849, 979, 1081, 1143, 1775
BshVI ATCGAT 1 cut(s) 1162
BsiSI CCGG 4 cut(s) 227, 536, 1221, 1317
BslFI GGGAC 2 cut(s) 185, 1874
BslI CCNNNNNNNGG 4 cut(s) 181, 1125, 1126, 1442
BsmAI GTCTC 1 cut(s) 89
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 185, 1874
BsnI GGCC 8 cut(s) 261, 379, 535, 849, 979, 1081, 1143, 1775
Bsp119I TTCGAA 1 cut(s) 1804
Bsp1286I GDGCHC 1 cut(s) 1582
Bsp1407I TGTACA 1 cut(s) 1617
BspACI CCGC 4 cut(s) 231, 481, 539, 720
BspANI GGCC 8 cut(s) 261, 379, 535, 849, 979, 1081, 1143, 1775
BspCNI CTCAG 2 cut(s) 114, 1790
BspDI ATCGAT 1 cut(s) 1162
BspLI GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
BspMAI CTGCAG 1 cut(s) 480
BspMI ACCTGC 1 cut(s) 1789
BspPI GGATC 4 cut(s) 561, 1138, 1240, 1499
BspT104I TTCGAA 1 cut(s) 1804
BsrFI RCCGGY 1 cut(s) 535
BsrGI TGTACA 1 cut(s) 1617
BsrI ACTGG 5 cut(s) 311, 742, 1082, 1684, 1904
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 6 cut(s) 137, 424, 649, 1101, 1394, 1990
BssNAI GTATAC 2 cut(s) 248, 1031
BssSI CACGAG 1 cut(s) 373
BssT1I CCWWGG 2 cut(s) 424, 1990
Bst1107I GTATAC 2 cut(s) 248, 1031
Bst2BI CACGAG 1 cut(s) 373
Bst2UI CCWGG 4 cut(s) 619, 1347, 1395, 1746
Bst4CI ACNGT 5 cut(s) 252, 457, 650, 1624, 1954
BstAPI GCANNNNNTGC 2 cut(s) 1196, 1787
BstAUI TGTACA 1 cut(s) 1617
BstBAI YACGTR 1 cut(s) 1498
BstBI TTCGAA 1 cut(s) 1804
BstC8I GCNNGC 3 cut(s) 537, 632, 1928
BstDEI CTNAG 3 cut(s) 122, 404, 1777
BstDSI CCRYGG 1 cut(s) 649
BstF5I GGATG 4 cut(s) 316, 819, 1023, 1467
BstH2I RGCGCY 1 cut(s) 523
BstHHI GCGC 1 cut(s) 522
BstMAI GTCTC 1 cut(s) 89
BstMWI GCNNNNNNNGC 2 cut(s) 1196, 1787
BstNI CCWGG 4 cut(s) 619, 1347, 1395, 1746
BstNSI RCATGY 1 cut(s) 634
BstPAI GACNNNNGTC 1 cut(s) 651
BstSCI CCNGG 7 cut(s) 226, 617, 1219, 1315, 1345, 1393, 1744
BstSFI CTRYAG 2 cut(s) 476, 1324
BstSLI GKGCMC 1 cut(s) 1582
BstSNI TACGTA 1 cut(s) 1498
BstV1I GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
BstX2I RGATCY 1 cut(s) 1504
BstYI RGATCY 1 cut(s) 1504
BstZ17I GTATAC 2 cut(s) 248, 1031
Bsu15I ATCGAT 1 cut(s) 1162
BsuI GTATCC 2 cut(s) 227, 1589
BsuRI GGCC 8 cut(s) 261, 379, 535, 849, 979, 1081, 1143, 1775
BsuTUI ATCGAT 1 cut(s) 1162
BtgI CCRYGG 1 cut(s) 649
BtgZI GCGATG 1 cut(s) 1836
BtrI CACGTC 1 cut(s) 1707
BtsCI GGATG 4 cut(s) 316, 819, 1023, 1467
BtsI GCAGTG 1 cut(s) 1479
BtsIMutI CAGTG 3 cut(s) 119, 1479, 2037
BveI ACCTGC 1 cut(s) 1789
Cac8I GCNNGC 3 cut(s) 537, 632, 1928
CfoI GCGC 1 cut(s) 522
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
ClaI ATCGAT 1 cut(s) 1162
DdeI CTNAG 3 cut(s) 122, 404, 1777
EaeI YGGCCR 2 cut(s) 533, 1079
EciI GGCGGA 1 cut(s) 246
Eco105I TACGTA 1 cut(s) 1498
Eco130I CCWWGG 2 cut(s) 424, 1990
Eco147I AGGCCT 1 cut(s) 261
Eco47I GGWCC 4 cut(s) 172, 862, 881, 1445
Eco57I CTGAAG 2 cut(s) 700, 917
EcoO109I RGGNCCY 3 cut(s) 172, 848, 862
EcoRI GAATTC 3 cut(s) 1018, 1490, 1884
EcoRII CCWGG 4 cut(s) 617, 1345, 1393, 1744
EcoT14I CCWWGG 2 cut(s) 424, 1990
EcoT22I ATGCAT 2 cut(s) 505, 1339
ErhI CCWWGG 2 cut(s) 424, 1990
Esp3I CGTCTC 1 cut(s) 89
FalI AAGNNNNNCTT 4 cut(s) 672, 704, 1183, 1215
FaqI GGGAC 2 cut(s) 185, 1874
FauI CCCGC 1 cut(s) 474
FauNDI CATATG 2 cut(s) 412, 505
FbaI TGATCA 1 cut(s) 816
FblI GTMKAC 3 cut(s) 247, 459, 1030
Fnu4HI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
FokI GGATG 4 cut(s) 323, 826, 1010, 1454
Fsp4HI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
FspBI CTAG 2 cut(s) 344, 2018
GlaI GCGC 1 cut(s) 521
GluI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
GsaI CCCAGC 3 cut(s) 828, 924, 1190
GsuI CTGGAG 1 cut(s) 1377
HaeII RGCGCY 1 cut(s) 523
HaeIII GGCC 8 cut(s) 261, 379, 535, 849, 979, 1081, 1143, 1775
HapII CCGG 4 cut(s) 227, 536, 1221, 1317
HhaI GCGC 1 cut(s) 522
Hin6I GCGC 1 cut(s) 520
HinP1I GCGC 1 cut(s) 520
HindIII AAGCTT 2 cut(s) 575, 681
HinfI GANTC 9 cut(s) 797, 807, 1177, 1270, 1293, 1543, 1944, 1995, 2042
HpaII CCGG 4 cut(s) 227, 536, 1221, 1317
HphI GGTGA 3 cut(s) 859, 965, 1991
Hpy166II GTNNAC 6 cut(s) 248, 460, 571, 1031, 1618, 1620
Hpy188I TCNGA 5 cut(s) 613, 663, 936, 1102, 2015
Hpy188III TCNNGA 4 cut(s) 319, 710, 811, 1072
Hpy8I GTNNAC 6 cut(s) 248, 460, 571, 1031, 1618, 1620
Hpy99I CGWCG 4 cut(s) 95, 103, 833, 1708
HpyAV CCTTC 7 cut(s) 409, 919, 944, 1122, 1280, 1934, 2037
HpyCH4III ACNGT 5 cut(s) 252, 457, 650, 1624, 1954
HpyCH4IV ACGT 4 cut(s) 237, 1497, 1582, 1706
HpyCH4V TGCA 6 cut(s) 478, 503, 1217, 1337, 1790, 1798
HpyF10VI GCNNNNNNNGC 2 cut(s) 1196, 1787
HpyF3I CTNAG 3 cut(s) 122, 404, 1777
HpySE526I ACGT 4 cut(s) 237, 1497, 1582, 1706
HspAI GCGC 1 cut(s) 520
KflI GGGWCCC 1 cut(s) 172
KroI GCCGGC 1 cut(s) 535
KroNI GCCGGC 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 816
LmnI GCTCC 2 cut(s) 126, 1009
Lsp1109I GCAGC 5 cut(s) 56, 490, 653, 1201, 1471
LweI GCATC 3 cut(s) 1033, 1047, 1476
MaeI CTAG 2 cut(s) 344, 2018
MaeII ACGT 4 cut(s) 237, 1497, 1582, 1706
MaeIII GTNAC 3 cut(s) 428, 1666, 1735
MboII GAAGA 7 cut(s) 19, 44, 47, 299, 959, 1107, 1295
MfeI CAATTG 1 cut(s) 1763
MflI RGATCY 1 cut(s) 1504
MhlI GDGCHC 1 cut(s) 1582
MlyI GAGTC 2 cut(s) 1186, 2004
MmeI TCCRAC 3 cut(s) 761, 908, 1538
Mph1103I ATGCAT 2 cut(s) 505, 1339
MroNI GCCGGC 1 cut(s) 535
MseI TTAA 5 cut(s) 855, 996, 1062, 1809, 1850
MspA1I CMGCKG 2 cut(s) 481, 720
MspI CCGG 4 cut(s) 227, 536, 1221, 1317
MspR9I CCNGG 7 cut(s) 228, 619, 1221, 1317, 1347, 1395, 1746
MunI CAATTG 1 cut(s) 1763
MvaI CCWGG 4 cut(s) 619, 1347, 1395, 1746
MwoI GCNNNNNNNGC 2 cut(s) 1196, 1787
NaeI GCCGGC 1 cut(s) 537
NciI CCSGG 3 cut(s) 228, 1221, 1317
NdeI CATATG 2 cut(s) 412, 505
NgoMIV GCCGGC 1 cut(s) 535
NlaIV GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
NmeAIII GCCGAG 2 cut(s) 118, 131
NmuCI GTSAC 2 cut(s) 1666, 1735
NsiI ATGCAT 2 cut(s) 505, 1339
NspI RCATGY 1 cut(s) 634
NspV TTCGAA 1 cut(s) 1804
PaeI GCATGC 1 cut(s) 634
PceI AGGCCT 1 cut(s) 261
PdiI GCCGGC 1 cut(s) 537
PfeI GAWTC 7 cut(s) 797, 807, 1270, 1293, 1543, 1944, 2042
PflMI CCANNNNNTGG 1 cut(s) 1442
PfoI TCCNGGA 1 cut(s) 1744
PkrI GCNGC 5 cut(s) 46, 480, 643, 1216, 1486
PleI GAGTC 2 cut(s) 1185, 2003
PpsI GAGTC 2 cut(s) 1185, 2003
Ppu21I YACGTR 1 cut(s) 1498
PpuMI RGGWCCY 2 cut(s) 172, 862
PshAI GACNNNNGTC 1 cut(s) 651
Psp5II RGGWCCY 2 cut(s) 172, 862
Psp6I CCWGG 4 cut(s) 617, 1345, 1393, 1744
PspFI CCCAGC 3 cut(s) 824, 920, 1186
PspGI CCWGG 4 cut(s) 617, 1345, 1393, 1744
PspN4I GGNNCC 5 cut(s) 173, 174, 850, 1005, 1344
PspPI GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
PspPPI RGGWCCY 2 cut(s) 172, 862
PsrI GAACNNNNNNTAC 2 cut(s) 1517, 1549
PstI CTGCAG 1 cut(s) 480
PsuI RGATCY 1 cut(s) 1504
SaqAI TTAA 5 cut(s) 855, 996, 1062, 1809, 1850
SatI GCNGC 5 cut(s) 45, 479, 642, 1215, 1485
Sau96I GGNCC 7 cut(s) 172, 848, 862, 881, 977, 1141, 1445
ScaI AGTACT 3 cut(s) 746, 790, 910
SchI GAGTC 2 cut(s) 1186, 2004
ScrFI CCNGG 7 cut(s) 228, 619, 1221, 1317, 1347, 1395, 1746
SduI GDGCHC 1 cut(s) 1582
SfaNI GCATC 3 cut(s) 1033, 1047, 1476
SfcI CTRYAG 2 cut(s) 476, 1324
SfuI TTCGAA 1 cut(s) 1804
SinI GGWCC 4 cut(s) 172, 862, 881, 1445
SmlI CTYRAG 2 cut(s) 1662, 1965
SmoI CTYRAG 2 cut(s) 1662, 1965
SnaBI TACGTA 1 cut(s) 1498
SphI GCATGC 1 cut(s) 634
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 4 cut(s) 231, 481, 539, 720
SspI AATATT 1 cut(s) 513
SspMI CTAG 2 cut(s) 344, 2018
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 7 cut(s) 226, 617, 1219, 1315, 1345, 1393, 1744
StyI CCWWGG 2 cut(s) 424, 1990
TaaI ACNGT 5 cut(s) 252, 457, 650, 1624, 1954
TaiI ACGT 4 cut(s) 240, 1500, 1585, 1709
TaqI TCGA 5 cut(s) 516, 753, 1162, 1312, 1804
TatI WGTACW 6 cut(s) 252, 744, 788, 908, 1617, 1896
TfiI GAWTC 7 cut(s) 797, 807, 1270, 1293, 1543, 1944, 2042
Tru1I TTAA 5 cut(s) 855, 996, 1062, 1809, 1850
Tru9I TTAA 5 cut(s) 855, 996, 1062, 1809, 1850
TscAI CASTG 3 cut(s) 126, 1486, 2044
TseFI GTSAC 2 cut(s) 1666, 1735
TseI GCWGC 5 cut(s) 44, 478, 641, 1214, 1484
Tsp45I GTSAC 2 cut(s) 1666, 1735
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 3 cut(s) 126, 1486, 2044
Van91I CCANNNNNTGG 1 cut(s) 1442
VpaK11BI GGWCC 4 cut(s) 172, 862, 881, 1445
XceI RCATGY 1 cut(s) 634
XmiI GTMKAC 3 cut(s) 247, 459, 1030
XspI CTAG 2 cut(s) 344, 2018
ZrmI AGTACT 3 cut(s) 746, 790, 910
Zsp2I ATGCAT 2 cut(s) 505, 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.