Rw2G016930

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000523
Physical Location & Seq
Forward (+)
20524060 .. 20528921
4862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G016930.1

Sequence Viewer

Length: 2019 bp
ATGGAGAAGATGAGCAAATGGGGTTGGCTTCTGGGGTCCTTAGCAGCAATGCTTCTTCTGGTTTTGCTCCTGGCTGGTCATGGAACTTACGAAGAGACGACGAGCCGAGTAAGGAACATAATTCCTCGGCGACAACAGGGGGTCAATTTACTAACAAGGGACCCCAAGAGGGTTGTTTTGGATAATGGCATTGTCAAAGTCATTTTCTCTAATCCGGGCGGATACGTTATTGGTATACAGTACAGAGGCCTTGACATACTTGAAATGGGGAATGAAGAAAATAATAGAGGGTACTGGGATGTGGTCTGGAAAAAGAATATAACTGACAAATTAGAAGCAACAAAATTTAGGGTTATTACCGCGAGGTCGGACCAAGCAGAAATTTCCTTCCTAAGAACATATGATGTTTCCCTTGGTAACGGCACGAACACAATTCCCTTGACCGTAGACAAAAGGTACATACTGCAGCGGGGTGGTTCTGGTTTCTATGCATATGGAATATTCGAGCGCCTTGATGGGTGGCCAGCGGTACAGGATATGGATCAAATTAGAATGGTTTACAAGCTTGAACAAGCCAAATTTCATTATATGGCGTTATCAGACACCAGGCAAAGAAGCATGCCAACAGCAGCAGACCGTGGTCGTGGTCAGAAACTTGCGTATAAAGAAGCTTGTCTTCTGACTAATCCAAGCAATCCTGACTTTAGAGGAGAGGACAAAGATAACAAAGTTCATGGTTGGATTAGTACTGATGAATCTGTGGGATTCTGGATGATCACACCCAGCGACGAATTTCGTACAGGAGGCCCCTTTAAGCAGGACCTCACCTCCCATGTTGGTCCGATCACCCTCTCCATGTTTGTTAGTACTCATTATGCTGGGAAGGAACTTCAGATCAAATTTGAAGAAGGTGAGGCATGGAAAAAGGTTTTTGGGCCTGTCTTTGTGTATCTTAATTCGGCTCCAAGCTCAAAGAATTCCATCCGTATACTTTGGAGGGATGCTAAAAAGCAGATGCTTAAAGAAGTCAAGAACTGGCCGTATAATTTTACTCAATCCGAGGACTTTCCTTCTTCCCATCAACGGGGATCAGTTTTGGGCCAATTACTAGTACATGATCGATACATCAATGAGAGTTTTTTCCCAGCAAGTTCTGCTTATGTGGGATTGGCTGCACCCGGAGAAGTGGGATCATGGCAGAGGGAAAACAAGGGTTATCAATTTTGGAATCAAGCTGACGAAGAAGGTAGATTCATTATCAAAAATGTTCGACCCGGAAACTACAGTTTTTATGCATGGGTTCCTGGCATTGTTGGGGATTACAAATATGAGGTTGATATTACAATTACTCCAGGGTCTAATATCAAATTGGACAATCTTACTTACGAACCTCCAAGAAATGGTCCTACTTTGTGGGAAATTGGCATCCCTGATCGCACTGCTGCCGAATTCTACGTACCAGATCCTTATCCAACTCTTTTGAACAATTTATACACCCAGAATCACACAGAAAATTTTAGGCAATACGGGTTGTGGGCACGTTATGGAGAGCGACACCCTCATAATGATCTTGTGTACACAGTAGCTATCAACAATTACCATGATGATTGGTTCTATGCTCAAGTGACCAGAAATACTGGAAATGAAACCTATGTAGGAACGACGTGGCAAATTCTATTTGAACTTCATAATGTCACAAATCCAGGAAATTATACACTCCAATTGGCACTGGCCTCAGCAAACAATGCAGAATTGCAGGTTCGAATTAACAACCAGAGCGATGAGCAACCTCATTTTTCATCAAAGTTGATTGGAGGGGACAATGCGATAGCAAGACATGGAATTCATGGTTTGTACTGGTTGTTTAGTGTTGAGGTATCAAGCTCGCTACTAAAAGAAGGAATCAACACCGTCTATCTTACTCAAGCGAGAGGTGGTGAAACTTTCCAAGGAGTCATGTATGACTACATCAGACTAGAAGCACCTTCATCACACACTGATTCACAAGTAGTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

672

Amino Acids

76.6

Weight (kDa)

6.38

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 44 - 238 4.9e-73 Rhamnogalacturonate lyase family
fn3_3 PF14686 384 - 457 4.8e-25 Polysaccharide lyase family 4, domain II
CBM-like PF14683 470 - 659 7e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1747
AccB7I CCANNNNNTGG 1 cut(s) 1400
AccI GTMKAC 3 cut(s) 235, 447, 988
AccII CGCG 1 cut(s) 362
AciI CCGC 4 cut(s) 219, 360, 469, 527
AclWI GGATC 4 cut(s) 549, 1096, 1198, 1457
AcoI YGGCCR 2 cut(s) 521, 1037
AcuI CTGAAG 1 cut(s) 875
AfiI CCNNNNNNNGG 4 cut(s) 169, 1083, 1084, 1400
AgsI TTSAA 5 cut(s) 263, 569, 905, 1483, 1682
AhlI ACTAGT 1 cut(s) 1108
AjiI CACGTC 1 cut(s) 1665
AjnI CCWGG 5 cut(s) 69, 605, 1303, 1351, 1702
AluBI AGCT 6 cut(s) 565, 671, 969, 1235, 1586, 1884
AluI AGCT 6 cut(s) 565, 671, 969, 1235, 1586, 1884
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 4 cut(s) 549, 1096, 1198, 1457
AoxI GGCC 7 cut(s) 247, 521, 805, 935, 1037, 1099, 1731
ApeKI GCWGC 5 cut(s) 44, 466, 629, 1172, 1442
AspLEI GCGC 1 cut(s) 510
AspS9I GGNCC 9 cut(s) 36, 160, 370, 806, 820, 839, 935, 1099, 1403
AsuC2I CCSGG 3 cut(s) 216, 1179, 1275
AsuHPI GGTGA 4 cut(s) 817, 838, 923, 1949
AsuII TTCGAA 1 cut(s) 1762
AvaII GGWCC 6 cut(s) 36, 160, 370, 820, 839, 1403
BaeGI GKGCMC 1 cut(s) 1540
BalI TGGCCA 1 cut(s) 523
BbsI GAAGAC 1 cut(s) 668
BbvCI CCTCAGC 1 cut(s) 1735
BbvI GCAGC 5 cut(s) 56, 478, 641, 1159, 1429
BccI CCATC 3 cut(s) 509, 989, 1086
BceAI ACGGC 2 cut(s) 436, 1024
BciT130I CCWGG 5 cut(s) 71, 607, 1305, 1353, 1704
BciVI GTATCC 1 cut(s) 215
BclI TGATCA 1 cut(s) 774
BcnI CCSGG 3 cut(s) 216, 1179, 1275
BcoDI GTCTC 1 cut(s) 89
BcuI ACTAGT 1 cut(s) 1108
BfaI CTAG 2 cut(s) 1109, 1976
BfmI CTRYAG 2 cut(s) 464, 1282
BfoI RGCGCY 1 cut(s) 511
BfuAI ACCTGC 1 cut(s) 1747
BfuI GTATCC 1 cut(s) 215
BisI GCNGC 5 cut(s) 45, 467, 630, 1173, 1443
BlsI GCNGC 5 cut(s) 46, 468, 631, 1174, 1444
BmcAI AGTACT 2 cut(s) 748, 868
Bme1390I CCNGG 8 cut(s) 71, 216, 607, 1179, 1275, 1305, 1353, 1704
Bme18I GGWCC 6 cut(s) 36, 160, 370, 820, 839, 1403
BmgBI CACGTC 1 cut(s) 1665
BmgT120I GGNCC 9 cut(s) 36, 160, 370, 806, 820, 839, 935, 1099, 1403
BmiI GGNNCC 6 cut(s) 37, 161, 162, 808, 963, 1302
BmrFI CCNGG 8 cut(s) 71, 216, 607, 1179, 1275, 1305, 1353, 1704
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 3 cut(s) 991, 1005, 1434
BmuI ACTGGG 1 cut(s) 304
BoxI GACNNNNGTC 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 668
BpmI CTGGAG 1 cut(s) 1335
Bpu10I CCTNAGC 2 cut(s) 40, 1735
Bpu14I TTCGAA 1 cut(s) 1762
BpuEI CTTGAG 2 cut(s) 1605, 1908
BpuMI CCSGG 3 cut(s) 216, 1179, 1275
Bsa29I ATCGAT 1 cut(s) 1120
BsaAI YACGTR 1 cut(s) 1456
BsaBI GATNNNNATC 2 cut(s) 540, 1467
BsaJI CCNNGG 6 cut(s) 125, 412, 637, 1059, 1352, 1948
BsaXI ACNNNNNCTCC 4 cut(s) 812, 842, 1333, 1363
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 1083, 1084, 1400
Bse1I ACTGG 5 cut(s) 299, 1040, 1642, 1734, 1862
Bse3DI GCAATG 1 cut(s) 54
Bse8I GATNNNNATC 2 cut(s) 540, 1467
BseBI CCWGG 5 cut(s) 71, 607, 1305, 1353, 1704
BseCI ATCGAT 1 cut(s) 1120
BseDI CCNNGG 6 cut(s) 125, 412, 637, 1059, 1352, 1948
BseGI GGATG 5 cut(s) 304, 777, 981, 1006, 1425
BseJI GATNNNNATC 2 cut(s) 540, 1467
BseLI CCNNNNNNNGG 4 cut(s) 169, 1083, 1084, 1400
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 1 cut(s) 1749
BseNI ACTGG 5 cut(s) 299, 1040, 1642, 1734, 1862
BseRI GAGGAG 1 cut(s) 723
BseSI GKGCMC 1 cut(s) 1540
BseXI GCAGC 5 cut(s) 56, 478, 641, 1159, 1429
BseYI CCCAGC 3 cut(s) 782, 878, 1144
BsgI GTGCAG 1 cut(s) 1158
Bsh1236I CGCG 1 cut(s) 362
BshFI GGCC 7 cut(s) 249, 523, 807, 937, 1039, 1101, 1733
BshVI ATCGAT 1 cut(s) 1120
BsiSI CCGG 3 cut(s) 215, 1179, 1275
BslFI GGGAC 2 cut(s) 173, 1832
BslI CCNNNNNNNGG 4 cut(s) 169, 1083, 1084, 1400
BsmAI GTCTC 1 cut(s) 89
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 173, 1832
BsnI GGCC 7 cut(s) 249, 523, 807, 937, 1039, 1101, 1733
Bsp119I TTCGAA 1 cut(s) 1762
Bsp1286I GDGCHC 1 cut(s) 1540
Bsp1407I TGTACA 1 cut(s) 1575
BspACI CCGC 4 cut(s) 219, 360, 469, 527
BspANI GGCC 7 cut(s) 249, 523, 807, 937, 1039, 1101, 1733
BspCNI CTCAG 1 cut(s) 1748
BspDI ATCGAT 1 cut(s) 1120
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 6 cut(s) 37, 161, 162, 808, 963, 1302
BspMAI CTGCAG 1 cut(s) 468
BspMI ACCTGC 1 cut(s) 1747
BspPI GGATC 4 cut(s) 549, 1096, 1198, 1457
BspT104I TTCGAA 1 cut(s) 1762
BsrDI GCAATG 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 1575
BsrI ACTGG 5 cut(s) 299, 1040, 1642, 1734, 1862
BssECI CCNNGG 6 cut(s) 125, 412, 637, 1059, 1352, 1948
BssNAI GTATAC 2 cut(s) 236, 989
BssT1I CCWWGG 2 cut(s) 412, 1948
Bst1107I GTATAC 2 cut(s) 236, 989
Bst2UI CCWGG 5 cut(s) 71, 607, 1305, 1353, 1704
Bst4CI ACNGT 6 cut(s) 240, 445, 638, 1286, 1582, 1912
Bst6I CTCTTC 1 cut(s) 87
BstAPI GCANNNNNTGC 2 cut(s) 1154, 1745
BstAUI TGTACA 1 cut(s) 1575
BstBAI YACGTR 1 cut(s) 1456
BstBI TTCGAA 1 cut(s) 1762
BstC8I GCNNGC 3 cut(s) 525, 620, 1886
BstDEI CTNAG 3 cut(s) 40, 392, 1735
BstDSI CCRYGG 1 cut(s) 637
BstF5I GGATG 5 cut(s) 304, 777, 981, 1006, 1425
BstFNI CGCG 1 cut(s) 362
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 1 cut(s) 510
BstMAI GTCTC 1 cut(s) 89
BstMWI GCNNNNNNNGC 2 cut(s) 1154, 1745
BstNI CCWGG 5 cut(s) 71, 607, 1305, 1353, 1704
BstNSI RCATGY 1 cut(s) 622
BstPAI GACNNNNGTC 1 cut(s) 639
BstSCI CCNGG 8 cut(s) 69, 214, 605, 1177, 1273, 1303, 1351, 1702
BstSFI CTRYAG 2 cut(s) 464, 1282
BstSLI GKGCMC 1 cut(s) 1540
BstSNI TACGTA 1 cut(s) 1456
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 5 cut(s) 56, 478, 641, 1159, 1429
BstV2I GAAGAC 1 cut(s) 668
BstX2I RGATCY 1 cut(s) 1462
BstYI RGATCY 1 cut(s) 1462
BstZ17I GTATAC 2 cut(s) 236, 989
Bsu15I ATCGAT 1 cut(s) 1120
BsuI GTATCC 1 cut(s) 215
BsuRI GGCC 7 cut(s) 249, 523, 807, 937, 1039, 1101, 1733
BsuTUI ATCGAT 1 cut(s) 1120
BtgI CCRYGG 1 cut(s) 637
BtgZI GCGATG 1 cut(s) 1794
BtrI CACGTC 1 cut(s) 1665
BtsCI GGATG 5 cut(s) 304, 777, 981, 1006, 1425
BtsI GCAGTG 1 cut(s) 1437
BtsIMutI CAGTG 3 cut(s) 1437, 1727, 1995
BveI ACCTGC 1 cut(s) 1747
Cac8I GCNNGC 3 cut(s) 525, 620, 1886
CfoI GCGC 1 cut(s) 510
Cfr13I GGNCC 9 cut(s) 36, 160, 370, 806, 820, 839, 935, 1099, 1403
ClaI ATCGAT 1 cut(s) 1120
DdeI CTNAG 3 cut(s) 40, 392, 1735
EaeI YGGCCR 2 cut(s) 521, 1037
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
EciI GGCGGA 1 cut(s) 234
Eco105I TACGTA 1 cut(s) 1456
Eco130I CCWWGG 2 cut(s) 412, 1948
Eco147I AGGCCT 1 cut(s) 249
Eco47I GGWCC 6 cut(s) 36, 160, 370, 820, 839, 1403
Eco57I CTGAAG 1 cut(s) 875
EcoO109I RGGNCCY 4 cut(s) 36, 160, 806, 820
EcoRI GAATTC 3 cut(s) 976, 1448, 1842
EcoRII CCWGG 5 cut(s) 69, 605, 1303, 1351, 1702
EcoT14I CCWWGG 2 cut(s) 412, 1948
EcoT22I ATGCAT 2 cut(s) 493, 1297
ErhI CCWWGG 2 cut(s) 412, 1948
Esp3I CGTCTC 1 cut(s) 89
FalI AAGNNNNNCTT 4 cut(s) 660, 692, 1141, 1173
FaqI GGGAC 2 cut(s) 173, 1832
FauI CCCGC 1 cut(s) 462
FauNDI CATATG 2 cut(s) 400, 493
FbaI TGATCA 1 cut(s) 774
FblI GTMKAC 3 cut(s) 235, 447, 988
Fnu4HI GCNGC 5 cut(s) 45, 467, 630, 1173, 1443
FokI GGATG 5 cut(s) 311, 784, 968, 1013, 1412
Fsp4HI GCNGC 5 cut(s) 45, 467, 630, 1173, 1443
FspBI CTAG 2 cut(s) 1109, 1976
GlaI GCGC 1 cut(s) 509
GluI GCNGC 5 cut(s) 45, 467, 630, 1173, 1443
GsaI CCCAGC 3 cut(s) 786, 882, 1148
GsuI CTGGAG 1 cut(s) 1335
HaeII RGCGCY 1 cut(s) 511
HaeIII GGCC 7 cut(s) 249, 523, 807, 937, 1039, 1101, 1733
HapII CCGG 3 cut(s) 215, 1179, 1275
HhaI GCGC 1 cut(s) 510
Hin6I GCGC 1 cut(s) 508
HinP1I GCGC 1 cut(s) 508
HindIII AAGCTT 2 cut(s) 563, 669
HinfI GANTC 8 cut(s) 755, 765, 1228, 1251, 1501, 1902, 1953, 2000
HpaII CCGG 3 cut(s) 215, 1179, 1275
HphI GGTGA 4 cut(s) 817, 838, 923, 1949
Hpy166II GTNNAC 6 cut(s) 236, 448, 559, 989, 1576, 1578
Hpy188I TCNGA 8 cut(s) 370, 601, 651, 681, 843, 894, 1060, 1973
Hpy188III TCNNGA 4 cut(s) 307, 698, 769, 1030
Hpy8I GTNNAC 6 cut(s) 236, 448, 559, 989, 1576, 1578
Hpy99I CGWCG 3 cut(s) 103, 791, 1666
HpyAV CCTTC 7 cut(s) 397, 877, 902, 1080, 1238, 1892, 1995
HpyCH4III ACNGT 6 cut(s) 240, 445, 638, 1286, 1582, 1912
HpyCH4IV ACGT 4 cut(s) 225, 1455, 1540, 1664
HpyCH4V TGCA 6 cut(s) 466, 491, 1175, 1295, 1748, 1756
HpyF10VI GCNNNNNNNGC 2 cut(s) 1154, 1745
HpyF3I CTNAG 3 cut(s) 40, 392, 1735
HpySE526I ACGT 4 cut(s) 225, 1455, 1540, 1664
HspAI GCGC 1 cut(s) 508
KflI GGGWCCC 1 cut(s) 160
Ksp22I TGATCA 1 cut(s) 774
LmnI GCTCC 2 cut(s) 72, 967
Lsp1109I GCAGC 5 cut(s) 56, 478, 641, 1159, 1429
LweI GCATC 3 cut(s) 991, 1005, 1434
MaeI CTAG 2 cut(s) 1109, 1976
MaeII ACGT 4 cut(s) 225, 1455, 1540, 1664
MaeIII GTNAC 3 cut(s) 416, 1624, 1693
MboII GAAGA 8 cut(s) 19, 47, 104, 287, 668, 917, 1065, 1253
MfeI CAATTG 1 cut(s) 1721
MflI RGATCY 1 cut(s) 1462
MhlI GDGCHC 1 cut(s) 1540
MlsI TGGCCA 1 cut(s) 523
MluNI TGGCCA 1 cut(s) 523
MlyI GAGTC 1 cut(s) 1962
MmeI TCCRAC 3 cut(s) 348, 719, 1496
Mox20I TGGCCA 1 cut(s) 523
Mph1103I ATGCAT 2 cut(s) 493, 1297
MscI TGGCCA 1 cut(s) 523
MseI TTAA 4 cut(s) 813, 954, 1020, 1767
Msp20I TGGCCA 1 cut(s) 523
MspA1I CMGCKG 2 cut(s) 469, 527
MspI CCGG 3 cut(s) 215, 1179, 1275
MspR9I CCNGG 8 cut(s) 71, 216, 607, 1179, 1275, 1305, 1353, 1704
MunI CAATTG 1 cut(s) 1721
MvaI CCWGG 5 cut(s) 71, 607, 1305, 1353, 1704
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 2 cut(s) 1154, 1745
NciI CCSGG 3 cut(s) 216, 1179, 1275
NdeI CATATG 2 cut(s) 400, 493
NlaIV GGNNCC 6 cut(s) 37, 161, 162, 808, 963, 1302
NmeAIII GCCGAG 2 cut(s) 106, 131
NmuCI GTSAC 2 cut(s) 1624, 1693
NsiI ATGCAT 2 cut(s) 493, 1297
NspI RCATGY 1 cut(s) 622
NspV TTCGAA 1 cut(s) 1762
PaeI GCATGC 1 cut(s) 622
PceI AGGCCT 1 cut(s) 249
PfeI GAWTC 7 cut(s) 755, 765, 1228, 1251, 1501, 1902, 2000
PflMI CCANNNNNTGG 1 cut(s) 1400
PfoI TCCNGGA 1 cut(s) 1702
PkrI GCNGC 5 cut(s) 46, 468, 631, 1174, 1444
PleI GAGTC 1 cut(s) 1961
PpsI GAGTC 1 cut(s) 1961
Ppu21I YACGTR 1 cut(s) 1456
PpuMI RGGWCCY 3 cut(s) 36, 160, 820
PshAI GACNNNNGTC 1 cut(s) 639
Psp5II RGGWCCY 3 cut(s) 36, 160, 820
Psp6I CCWGG 5 cut(s) 69, 605, 1303, 1351, 1702
PspFI CCCAGC 3 cut(s) 782, 878, 1144
PspGI CCWGG 5 cut(s) 69, 605, 1303, 1351, 1702
PspN4I GGNNCC 6 cut(s) 37, 161, 162, 808, 963, 1302
PspPI GGNCC 9 cut(s) 36, 160, 370, 806, 820, 839, 935, 1099, 1403
PspPPI RGGWCCY 3 cut(s) 36, 160, 820
PsrI GAACNNNNNNTAC 2 cut(s) 1475, 1507
PstI CTGCAG 1 cut(s) 468
PsuI RGATCY 1 cut(s) 1462
SaqAI TTAA 4 cut(s) 813, 954, 1020, 1767
SatI GCNGC 5 cut(s) 45, 467, 630, 1173, 1443
Sau96I GGNCC 9 cut(s) 36, 160, 370, 806, 820, 839, 935, 1099, 1403
ScaI AGTACT 2 cut(s) 748, 868
SchI GAGTC 1 cut(s) 1962
ScrFI CCNGG 8 cut(s) 71, 216, 607, 1179, 1275, 1305, 1353, 1704
SduI GDGCHC 1 cut(s) 1540
SfaNI GCATC 3 cut(s) 991, 1005, 1434
SfcI CTRYAG 2 cut(s) 464, 1282
SfuI TTCGAA 1 cut(s) 1762
SinI GGWCC 6 cut(s) 36, 160, 370, 820, 839, 1403
SmlI CTYRAG 2 cut(s) 1620, 1923
SmoI CTYRAG 2 cut(s) 1620, 1923
SnaBI TACGTA 1 cut(s) 1456
SpeI ACTAGT 1 cut(s) 1108
SphI GCATGC 1 cut(s) 622
SseBI AGGCCT 1 cut(s) 249
SsiI CCGC 4 cut(s) 219, 360, 469, 527
SspI AATATT 1 cut(s) 501
SspMI CTAG 2 cut(s) 1109, 1976
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 8 cut(s) 69, 214, 605, 1177, 1273, 1303, 1351, 1702
StyI CCWWGG 2 cut(s) 412, 1948
TaaI ACNGT 6 cut(s) 240, 445, 638, 1286, 1582, 1912
TaiI ACGT 4 cut(s) 228, 1458, 1543, 1667
TaqI TCGA 4 cut(s) 504, 1120, 1270, 1762
TatI WGTACW 6 cut(s) 240, 746, 866, 1111, 1575, 1854
TfiI GAWTC 7 cut(s) 755, 765, 1228, 1251, 1501, 1902, 2000
Tru1I TTAA 4 cut(s) 813, 954, 1020, 1767
Tru9I TTAA 4 cut(s) 813, 954, 1020, 1767
TscAI CASTG 3 cut(s) 1444, 1734, 2002
TseFI GTSAC 2 cut(s) 1624, 1693
TseI GCWGC 5 cut(s) 44, 466, 629, 1172, 1442
Tsp45I GTSAC 2 cut(s) 1624, 1693
TspGWI ACGGA 1 cut(s) 974
TspRI CASTG 3 cut(s) 1444, 1734, 2002
Van91I CCANNNNNTGG 1 cut(s) 1400
VpaK11BI GGWCC 6 cut(s) 36, 160, 370, 820, 839, 1403
XceI RCATGY 1 cut(s) 622
XmiI GTMKAC 3 cut(s) 235, 447, 988
XspI CTAG 2 cut(s) 1109, 1976
ZrmI AGTACT 2 cut(s) 748, 868
Zsp2I ATGCAT 2 cut(s) 493, 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.