MD01G1043300.v1.1

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000523
Physical Location & Seq
Reverse (-)
14194872 .. 14201482
6611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1043300.v1.1.491

Sequence Viewer

Length: 1986 bp
ATGCTCGCTTTGCTCTTCTTCTTGCCTGATGATGGTAACTTCGCGAAGATTTCCGTAAGAAGAGCACTAAGGAGCGTTACTGGATCAAATAATGGGCTCGACCAGATGCTAATAAAGACTAAAAACCAGGTGGTAATGGATAATGGCATTGTCCGGCTTACGTTTTCTTATCCCGGAGGAGACGTCATCGGAATCAAGTACAAGGGAATCGACAACTTACTTGAAATCAAGAATCAGGAAAATAATAGAGGGTATTGGGATGTTGTCTGGAATAACAAAGAGACAGGAGGTATCGACAAACTACAAGGGAGGAGATTTAAAATTATTACTGCGAGGGCAGATCAAACAGAAATTTCTTTTACCAAAACATACGATATTTCTCGAGGAAATGCCACACTTTCCATGAATGTTGACAAAAGGTACATAATGCAGCGTGGTCGCGCCGGGTTCTATGCGTATTCCATATTTGAGCGTCCCAAAGGGTGGCCGGGATTGGACATCGATCAAATTAGAATTGTGTACAAACTTCAACAAGACAAATTTCAATTCATGGCTGTGTCTGACGACAGACAAAGGGTCATGCCAACAGCCGATGACCGTTCAAACGGTATTAAACTTGCCTATCCTGAAGCTGTTCTTCTGACCAATCCAAGCAATCCTGACCTCAGAGGAGAGGTGGACGATAAGTACCAATACTCAAGTGAGGACAAAGATAACAAGGTCCATGGATGGATTTGCACTAAGCCAGCTGTAGGCTTCTGGATCATCACACCTAGTGATGAGTTTCGCACAGCTGGGCCCTTCAAGCAGGACCTCACCTCTCATGTAGGCCCAACCGCCCTCTCAATGTTTGTTAGTACTCACTATGCTGGGAAGGAGGTTGGGATGAGATTTGCAAGAGGTGAGGCATGGAAGAAAGTTTTTGGGCCTGTCTTTGTGTATCTCAATTCAGTTCCAAGTTTAAATGAGTCCGCTCTCTGGGAAAACGCTAAAGAGCAGTTGGTTGAAGAAGTCAAGAGCTGGCCATACAGTTTCACTCAATCAGAAGACATTCCTAGCTCAAGCGGACGCGGATCAGTTGCCGGTCAATTACTAGTACGGGATCGGTACACCAGCAAGAGCCTTGTTTGGGCAAGTTCTGCATATGTAGGATTGGCTGCACCTGGAAAAGCGGGATCATGGCAAAAGGAAAGCAAGGGTTATCAATTCTGGACTCAAGCTAACGAGCAAGGTCATTTCCTCATTAAAGATGTTCGACCTGGAAATTATAGTTTGTATGCAACTGTTCCCGGCATTATTGGGGATTACAAATTTAAGTCTAATATTACAATTGAACCAGGAAGTGAAATCAATTTGGCTAATCTTACCTATGTACCTCCAAGAAATGGTCCCACCCTGTGGGAAATTGGCATACCGGATCGATCCGCGGCCGAGTTTTACGTACCGGATCCAAATCCCACCCTTATGAACAAATTGTTCACCACCAATCGCGCACACAAGTTTAGGCAATATGGCTTGTGGGAACGATATGCAGATTTATACCCTGATGAAGATCTCATCTATAACATTGGTACTGATAATTATAGTGATAACTGGTTTTTCGCCCATGTGACGAGGAACATCGGAAATCGTACGTATGAAGCAACCACATGGAGAATTCTTTTTGAACTTCAAGATTTGACAAATACCGGAAATTATACACTCCAATTGGCATTGGCCTCAGCCACTAATGCAGAAGTGCAGGTTCGATTCAACGATCAGAGCGCCTTCCCACCTCATTTTACGACAGGGCTAATAGGGAAGGACAATGCCATAGCTAGGCATGGAATTCATGGTTTGTACTGGTTGTTCAGTATTGGTGTACCAAGCTACCGACTACGAGAAGGAAACAACACCATCTATCTTACTCAGTCAAGAAGCTTGGGCACTTTCGAAGGAGTGATGTACGACTATATCCGACTAGAAGGACCTCCTCCTCGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

662

Amino Acids

74.92

Weight (kDa)

8.74

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 33 - 226 3.6e-80 Rhamnogalacturonate lyase family
fn3_3 PF14686 379 - 452 1.5e-23 Polysaccharide lyase family 4, domain II
CBM-like PF14683 465 - 654 2.1e-48 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000523)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65210 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT2G22620 AT4G37950 AT4G37950 AT4G38030
fragaria_vesca FvH4_1g03290 FvH4_1g03300 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19194 FvH4_1g19200
malus_domestica MD01G1043200.v1.1 MD01G1043300.v1.1 MD02G1298500.v1.1 MD02G1307100.v1.1 MD04G1240500.v1.1 MD12G1256900.v1.1 MD12G1257400.v1.1 MD12G1257500.v1.1 MD15G1308100.v1.1 MD15G1308200.v1.1
prunus_persica Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1 Prupe.6G208400_v2.0.a1
pyrus_communis pycom01g07080 pycom15g27240
rosa_chinensis RchiOBHm_Chr2g0088421 RchiOBHm_Chr2g0088461 RchiOBHm_Chr2g0088481 RchiOBHm_Chr2g0110401 RchiOBHm_Chr2g0110411 RchiOBHm_Chr2g0110431
rosa_laevigata RLG00000003093 RLG00000015953 RLG00000015956 RLG00000015959 RLG00000017802 RLG00000017806
rosa_multiflora Rmu_sc0001393.1_g000003 Rmu_sc0001393.1_g000009 Rmu_sc0004733.1_g000013 Rmu_sc0004733.1_g000022
rosa_roxburghii Rroxscaffold_2G00133400 Rroxscaffold_2G00133420 Rroxscaffold_2G00152640 Rroxscaffold_2G00152680
rosa_rugosa Rorug01G0484000 Rorug01G0484200 Rorug01G0484600 Rorug02G0166800 Rorug02G0166900 Rorug02G0167000 Rorug02G0167500 Rorug02G0167600 Rorug02G0167800 Rorug02G0167800 Rorug07G0116100
rosa_samantha Rh2AG038500 Rh2AG038800 Rh2AG039000 Rh2AG219300 Rh2AG219400 Rh2BG038100 Rh2BG038400 Rh2BG228800 Rh2BG229000 Rh2CG039000 Rh2CG039600 Rh2CG221000 Rh2CG221200 Rh2DG038600 Rh2DG038800 Rh2DG225500 Rh2DG225600
rosa_wichuraiana Rw2G003060 Rw2G003080 Rw2G016930 Rw2G016940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
Acc36I ACCTGC 1 cut(s) 1732
AccB7I CCANNNNNTGG 3 cut(s) 483, 1385, 1398
AccBSI CCGCTC 1 cut(s) 974
AccII CGCG 5 cut(s) 44, 441, 1071, 1427, 1491
AciI CCGC 7 cut(s) 837, 972, 1065, 1071, 1172, 1425, 1427
AclWI GGATC 9 cut(s) 91, 770, 1081, 1110, 1183, 1416, 1425, 1442, 1455
AcoI YGGCCR 3 cut(s) 485, 1022, 1428
AcsI RAATTY 5 cut(s) 351, 539, 1310, 1656, 1827
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 183
AdeI CACNNNGTG 2 cut(s) 776, 1398
AfiI CCNNNNNNNGG 8 cut(s) 32, 483, 752, 978, 1129, 1385, 1398, 1818
AhdI GACNNNNNGTC 1 cut(s) 575
AhlI ACTAGT 1 cut(s) 1093
AjnI CCWGG 4 cut(s) 126, 1162, 1258, 1336
AluBI AGCT 9 cut(s) 632, 749, 794, 1020, 1059, 1220, 1817, 1869, 1920
AluI AGCT 9 cut(s) 632, 749, 794, 1020, 1059, 1220, 1817, 1869, 1920
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 2 cut(s) 174, 275
AlwI GGATC 9 cut(s) 91, 770, 1081, 1110, 1183, 1416, 1425, 1442, 1455
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 7 cut(s) 485, 797, 829, 926, 1022, 1428, 1716
ApaI GGGCCC 1 cut(s) 801
ApeKI GCWGC 2 cut(s) 430, 1157
ApoI RAATTY 5 cut(s) 351, 539, 1310, 1656, 1827
Asp700I GAANNNNTTC 2 cut(s) 633, 1050
AspLEI GCGC 3 cut(s) 443, 1493, 1766
AspS9I GGNCC 8 cut(s) 721, 797, 798, 811, 830, 926, 1388, 1967
AsuC2I CCSGG 4 cut(s) 174, 445, 489, 1290
AsuHPI GGTGA 3 cut(s) 808, 914, 1471
AsuII TTCGAA 1 cut(s) 1932
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 4 cut(s) 721, 811, 1388, 1967
BaeGI GKGCMC 2 cut(s) 801, 1928
BaeI ACNNNNGTAYC 2 cut(s) 1356, 1389
BalI TGGCCA 1 cut(s) 1024
BamHI GGATCC 1 cut(s) 1447
BanII GRGCYC 2 cut(s) 99, 801
BbsI GAAGAC 1 cut(s) 1053
Bbv12I GWGCWC 1 cut(s) 67
BbvCI CCTCAGC 1 cut(s) 1720
BbvI GCAGC 2 cut(s) 442, 1144
BccI CCATC 3 cut(s) 26, 723, 1904
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 4 cut(s) 128, 1164, 1260, 1338
BcnI CCSGG 4 cut(s) 174, 445, 489, 1290
BcoDI GTCTC 2 cut(s) 174, 275
BcuI ACTAGT 1 cut(s) 1093
BfaI CTAG 5 cut(s) 774, 1056, 1094, 1818, 1961
BfmI CTRYAG 1 cut(s) 750
BfoI RGCGCY 1 cut(s) 1767
BfuAI ACCTGC 1 cut(s) 1732
BglII AGATCT 1 cut(s) 1552
BisI GCNGC 3 cut(s) 431, 1158, 1428
BlsI GCNGC 3 cut(s) 432, 1159, 1429
BmcAI AGTACT 1 cut(s) 859
Bme1390I CCNGG 8 cut(s) 128, 174, 445, 489, 1164, 1260, 1290, 1338
Bme18I GGWCC 4 cut(s) 721, 811, 1388, 1967
BmeRI GACNNNNNGTC 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 8 cut(s) 721, 797, 798, 811, 830, 926, 1388, 1967
BmiI GGNNCC 3 cut(s) 799, 1390, 1449
BmrFI CCNGG 8 cut(s) 128, 174, 445, 489, 1164, 1260, 1290, 1338
BmsI GCATC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 1053
Bpu10I CCTNAGC 1 cut(s) 1720
Bpu14I TTCGAA 1 cut(s) 1932
BpuEI CTTGAG 3 cut(s) 682, 1045, 1200
BpuMI CCSGG 4 cut(s) 174, 445, 489, 1290
Bsa29I ATCGAT 2 cut(s) 501, 1420
BsaAI YACGTR 2 cut(s) 1441, 1635
BsaBI GATNNNNATC 2 cut(s) 1452, 1551
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 724, 1425
BsaWI WCCGGW 3 cut(s) 1414, 1444, 1688
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 8 cut(s) 32, 483, 752, 978, 1129, 1385, 1398, 1818
Bse118I RCCGGY 1 cut(s) 1082
Bse1I ACTGG 3 cut(s) 85, 1598, 1847
Bse8I GATNNNNATC 2 cut(s) 1452, 1551
BseBI CCWGG 4 cut(s) 128, 1164, 1260, 1338
BseCI ATCGAT 2 cut(s) 501, 1420
BseDI CCNNGG 2 cut(s) 724, 1425
BseGI GGATG 3 cut(s) 265, 734, 891
BseJI GATNNNNATC 2 cut(s) 1452, 1551
BseLI CCNNNNNNNGG 8 cut(s) 32, 483, 752, 978, 1129, 1385, 1398, 1818
BseMII CTCAG 3 cut(s) 679, 1734, 1922
BseNI ACTGG 3 cut(s) 85, 1598, 1847
BseRI GAGGAG 5 cut(s) 192, 325, 684, 1962, 1965
BseSI GKGCMC 2 cut(s) 801, 1928
BseX3I CGGCCG 1 cut(s) 1428
BseXI GCAGC 2 cut(s) 442, 1144
BseYI CCCAGC 2 cut(s) 794, 869
BsgI GTGCAG 2 cut(s) 1143, 1760
Bsh1236I CGCG 5 cut(s) 44, 441, 1071, 1427, 1491
Bsh1285I CGRYCG 1 cut(s) 1431
BshFI GGCC 7 cut(s) 487, 799, 831, 928, 1024, 1430, 1718
BshVI ATCGAT 2 cut(s) 501, 1420
BsiEI CGRYCG 1 cut(s) 1431
BsiHKAI GWGCWC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 381
BsiSI CCGG 9 cut(s) 154, 174, 444, 488, 1083, 1290, 1415, 1445, 1689
BsiWI CGTACG 1 cut(s) 1631
BslFI GGGAC 2 cut(s) 459, 1374
BslI CCNNNNNNNGG 8 cut(s) 32, 483, 752, 978, 1129, 1385, 1398, 1818
BsmAI GTCTC 2 cut(s) 174, 275
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 2 cut(s) 459, 1374
BsnI GGCC 7 cut(s) 487, 799, 831, 928, 1024, 1430, 1718
BsoBI CYCGRG 1 cut(s) 381
Bsp119I TTCGAA 1 cut(s) 1932
Bsp120I GGGCCC 1 cut(s) 797
Bsp1286I GDGCHC 4 cut(s) 67, 99, 801, 1928
Bsp1407I TGTACA 1 cut(s) 519
Bsp19I CCATGG 1 cut(s) 724
Bsp68I TCGCGA 1 cut(s) 44
BspACI CCGC 7 cut(s) 837, 972, 1065, 1071, 1172, 1425, 1427
BspANI GGCC 7 cut(s) 487, 799, 831, 928, 1024, 1430, 1718
BspCNI CTCAG 3 cut(s) 678, 1733, 1921
BspDI ATCGAT 2 cut(s) 501, 1420
BspFNI CGCG 5 cut(s) 44, 441, 1071, 1427, 1491
BspLI GGNNCC 3 cut(s) 799, 1390, 1449
BspMI ACCTGC 1 cut(s) 1732
BspPI GGATC 9 cut(s) 91, 770, 1081, 1110, 1183, 1416, 1425, 1442, 1455
BspQI GCTCTTC 2 cut(s) 20, 55
BspT104I TTCGAA 1 cut(s) 1932
BsrBI CCGCTC 1 cut(s) 974
BsrFI RCCGGY 1 cut(s) 1082
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 3 cut(s) 85, 1598, 1847
BssAI RCCGGY 1 cut(s) 1082
BssECI CCNNGG 2 cut(s) 724, 1425
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 724
Bst2UI CCWGG 4 cut(s) 128, 1164, 1260, 1338
Bst4CI ACNGT 4 cut(s) 599, 608, 1031, 1285
Bst6I CTCTTC 2 cut(s) 20, 55
BstACI GRCGYC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 1139
BstAUI TGTACA 1 cut(s) 519
BstBAI YACGTR 2 cut(s) 1441, 1635
BstBI TTCGAA 1 cut(s) 1932
BstC8I GCNNGC 3 cut(s) 6, 747, 1022
BstDEI CTNAG 5 cut(s) 68, 665, 741, 1720, 1908
BstDSI CCRYGG 2 cut(s) 724, 1425
BstF5I GGATG 3 cut(s) 265, 734, 891
BstFNI CGCG 5 cut(s) 44, 441, 1071, 1427, 1491
BstH2I RGCGCY 1 cut(s) 1767
BstHHI GCGC 3 cut(s) 443, 1493, 1766
BstMAI GTCTC 2 cut(s) 174, 275
BstMCI CGRYCG 1 cut(s) 1431
BstMWI GCNNNNNNNGC 4 cut(s) 10, 805, 1139, 1730
BstNI CCWGG 4 cut(s) 128, 1164, 1260, 1338
BstSCI CCNGG 8 cut(s) 126, 172, 443, 487, 1162, 1258, 1288, 1336
BstSFI CTRYAG 1 cut(s) 750
BstSLI GKGCMC 2 cut(s) 801, 1928
BstSNI TACGTA 2 cut(s) 1441, 1635
BstUI CGCG 5 cut(s) 44, 441, 1071, 1427, 1491
BstV1I GCAGC 2 cut(s) 442, 1144
BstV2I GAAGAC 1 cut(s) 1053
BstX2I RGATCY 2 cut(s) 1447, 1552
BstYI RGATCY 2 cut(s) 1447, 1552
BstZI CGGCCG 1 cut(s) 1428
Bsu15I ATCGAT 2 cut(s) 501, 1420
BsuRI GGCC 7 cut(s) 487, 799, 831, 928, 1024, 1430, 1718
BsuTUI ATCGAT 2 cut(s) 501, 1420
BtgI CCRYGG 2 cut(s) 724, 1425
BtsCI GGATG 3 cut(s) 265, 734, 891
BtuMI TCGCGA 1 cut(s) 44
BveI ACCTGC 1 cut(s) 1732
Cac8I GCNNGC 3 cut(s) 6, 747, 1022
CfoI GCGC 3 cut(s) 443, 1493, 1766
Cfr10I RCCGGY 1 cut(s) 1082
Cfr13I GGNCC 8 cut(s) 721, 797, 798, 811, 830, 926, 1388, 1967
Cfr42I CCGCGG 1 cut(s) 1428
ClaI ATCGAT 2 cut(s) 501, 1420
CseI GACGC 2 cut(s) 461, 1077
CsiI ACCWGGT 1 cut(s) 126
DdeI CTNAG 5 cut(s) 68, 665, 741, 1720, 1908
DraI TTTAAA 2 cut(s) 319, 963
DraIII CACNNNGTG 2 cut(s) 776, 1398
DriI GACNNNNNGTC 1 cut(s) 575
EaeI YGGCCR 3 cut(s) 485, 1022, 1428
EagI CGGCCG 1 cut(s) 1428
Eam1104I CTCTTC 2 cut(s) 20, 55
Eam1105I GACNNNNNGTC 1 cut(s) 575
EarI CTCTTC 2 cut(s) 20, 55
EclXI CGGCCG 1 cut(s) 1428
Eco105I TACGTA 2 cut(s) 1441, 1635
Eco130I CCWWGG 1 cut(s) 724
Eco24I GRGCYC 2 cut(s) 99, 801
Eco47I GGWCC 4 cut(s) 721, 811, 1388, 1967
Eco52I CGGCCG 1 cut(s) 1428
Eco57I CTGAAG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 381
EcoO109I RGGNCCY 3 cut(s) 798, 811, 1967
EcoRI GAATTC 2 cut(s) 1656, 1827
EcoRII CCWGG 4 cut(s) 126, 1162, 1258, 1336
EcoT14I CCWWGG 1 cut(s) 724
EcoT38I GRGCYC 2 cut(s) 99, 801
ErhI CCWWGG 1 cut(s) 724
Esp3I CGTCTC 1 cut(s) 174
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FaqI GGGAC 2 cut(s) 459, 1374
FauI CCCGC 1 cut(s) 1165
FauNDI CATATG 1 cut(s) 1144
Fnu4HI GCNGC 3 cut(s) 431, 1158, 1428
FokI GGATG 3 cut(s) 272, 741, 898
FriOI GRGCYC 2 cut(s) 99, 801
Fsp4HI GCNGC 3 cut(s) 431, 1158, 1428
FspBI CTAG 5 cut(s) 774, 1056, 1094, 1818, 1961
GlaI GCGC 3 cut(s) 442, 1492, 1765
GluI GCNGC 3 cut(s) 431, 1158, 1428
GsaI CCCAGC 2 cut(s) 798, 873
HaeII RGCGCY 1 cut(s) 1767
HaeIII GGCC 7 cut(s) 487, 799, 831, 928, 1024, 1430, 1718
HapII CCGG 9 cut(s) 154, 174, 444, 488, 1083, 1290, 1415, 1445, 1689
HgaI GACGC 2 cut(s) 461, 1077
HhaI GCGC 3 cut(s) 443, 1493, 1766
Hin1I GRCGYC 1 cut(s) 183
Hin6I GCGC 3 cut(s) 441, 1491, 1764
HinP1I GCGC 3 cut(s) 441, 1491, 1764
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HindIII AAGCTT 1 cut(s) 1918
HinfI GANTC 6 cut(s) 192, 207, 232, 968, 1213, 1749
HpaII CCGG 9 cut(s) 154, 174, 444, 488, 1083, 1290, 1415, 1445, 1689
HphI GGTGA 3 cut(s) 808, 914, 1471
Hpy166II GTNNAC 6 cut(s) 412, 520, 679, 1110, 1479, 1862
Hpy188I TCNGA 8 cut(s) 191, 562, 642, 668, 1045, 1625, 1761, 1958
Hpy8I GTNNAC 6 cut(s) 412, 520, 679, 1110, 1479, 1862
HpyAV CCTTC 7 cut(s) 811, 868, 1777, 1795, 1877, 1928, 1958
HpyCH4III ACNGT 4 cut(s) 599, 608, 1031, 1285
HpyCH4IV ACGT 4 cut(s) 161, 183, 1440, 1634
HpyCH4V TGCA 9 cut(s) 430, 738, 896, 1142, 1160, 1280, 1532, 1733, 1741
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 805, 1139, 1730
HpyF3I CTNAG 5 cut(s) 68, 665, 741, 1720, 1908
HpySE526I ACGT 4 cut(s) 161, 183, 1440, 1634
Hsp92I GRCGYC 1 cut(s) 183
HspAI GCGC 3 cut(s) 441, 1491, 1764
KspI CCGCGG 1 cut(s) 1428
LguI GCTCTTC 2 cut(s) 20, 55
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 2 cut(s) 442, 1144
LweI GCATC 1 cut(s) 96
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 5 cut(s) 774, 1056, 1094, 1818, 1961
MaeII ACGT 4 cut(s) 161, 183, 1440, 1634
MaeIII GTNAC 3 cut(s) 35, 76, 1609
MbiI CCGCTC 1 cut(s) 974
MboII GAAGA 9 cut(s) 7, 10, 58, 72, 629, 925, 1019, 1058, 1562
MfeI CAATTG 2 cut(s) 1329, 1706
MflI RGATCY 2 cut(s) 1447, 1552
MhlI GDGCHC 4 cut(s) 67, 99, 801, 1928
MlsI TGGCCA 1 cut(s) 1024
MluNI TGGCCA 1 cut(s) 1024
MlyI GAGTC 2 cut(s) 977, 1207
MmeI TCCRAC 1 cut(s) 1981
Mox20I TGGCCA 1 cut(s) 1024
MroXI GAANNNNTTC 2 cut(s) 633, 1050
MscI TGGCCA 1 cut(s) 1024
MseI TTAA 5 cut(s) 318, 612, 962, 1245, 1314
MslI CAYNNNNRTG 2 cut(s) 554, 1463
Msp20I TGGCCA 1 cut(s) 1024
MspA1I CMGCKG 3 cut(s) 749, 794, 1427
MspI CCGG 9 cut(s) 154, 174, 444, 488, 1083, 1290, 1415, 1445, 1689
MspR9I CCNGG 8 cut(s) 128, 174, 445, 489, 1164, 1260, 1290, 1338
MunI CAATTG 2 cut(s) 1329, 1706
MvaI CCWGG 4 cut(s) 128, 1164, 1260, 1338
MvnI CGCG 5 cut(s) 44, 441, 1071, 1427, 1491
MwoI GCNNNNNNNGC 4 cut(s) 10, 805, 1139, 1730
NciI CCSGG 4 cut(s) 174, 445, 489, 1290
NcoI CCATGG 1 cut(s) 724
NdeI CATATG 1 cut(s) 1144
NlaIV GGNNCC 3 cut(s) 799, 1390, 1449
NmeAIII GCCGAG 1 cut(s) 1456
NmuCI GTSAC 1 cut(s) 1609
NruI TCGCGA 1 cut(s) 44
NspV TTCGAA 1 cut(s) 1932
PaeR7I CTCGAG 1 cut(s) 381
PciSI GCTCTTC 2 cut(s) 20, 55
PdmI GAANNNNTTC 2 cut(s) 633, 1050
PfeI GAWTC 4 cut(s) 192, 207, 232, 1749
Pfl23II CGTACG 1 cut(s) 1631
PflMI CCANNNNNTGG 3 cut(s) 483, 1385, 1398
PfoI TCCNGGA 1 cut(s) 172
PkrI GCNGC 3 cut(s) 432, 1159, 1429
PleI GAGTC 2 cut(s) 976, 1207
PpsI GAGTC 2 cut(s) 976, 1207
Ppu21I YACGTR 2 cut(s) 1441, 1635
PpuMI RGGWCCY 2 cut(s) 811, 1967
Psp5II RGGWCCY 2 cut(s) 811, 1967
Psp6I CCWGG 4 cut(s) 126, 1162, 1258, 1336
PspFI CCCAGC 2 cut(s) 794, 869
PspGI CCWGG 4 cut(s) 126, 1162, 1258, 1336
PspLI CGTACG 1 cut(s) 1631
PspN4I GGNNCC 3 cut(s) 799, 1390, 1449
PspOMI GGGCCC 1 cut(s) 797
PspPI GGNCC 8 cut(s) 721, 797, 798, 811, 830, 926, 1388, 1967
PspPPI RGGWCCY 2 cut(s) 811, 1967
PsrI GAACNNNNNNTAC 2 cut(s) 1832, 1864
PsuI RGATCY 2 cut(s) 1447, 1552
PvuII CAGCTG 2 cut(s) 749, 794
RruI TCGCGA 1 cut(s) 44
RseI CAYNNNNRTG 2 cut(s) 554, 1463
SacII CCGCGG 1 cut(s) 1428
SapI GCTCTTC 2 cut(s) 20, 55
SaqAI TTAA 5 cut(s) 318, 612, 962, 1245, 1314
SatI GCNGC 3 cut(s) 431, 1158, 1428
Sau96I GGNCC 8 cut(s) 721, 797, 798, 811, 830, 926, 1388, 1967
ScaI AGTACT 1 cut(s) 859
SchI GAGTC 2 cut(s) 977, 1207
ScrFI CCNGG 8 cut(s) 128, 174, 445, 489, 1164, 1260, 1290, 1338
SduI GDGCHC 4 cut(s) 67, 99, 801, 1928
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 1 cut(s) 96
SfcI CTRYAG 1 cut(s) 750
Sfr274I CTCGAG 1 cut(s) 381
Sfr303I CCGCGG 1 cut(s) 1428
SfuI TTCGAA 1 cut(s) 1932
SgrBI CCGCGG 1 cut(s) 1428
SinI GGWCC 4 cut(s) 721, 811, 1388, 1967
SlaI CTCGAG 1 cut(s) 381
SmiMI CAYNNNNRTG 2 cut(s) 554, 1463
SmlI CTYRAG 4 cut(s) 381, 697, 1060, 1215
SmoI CTYRAG 4 cut(s) 381, 697, 1060, 1215
SnaBI TACGTA 2 cut(s) 1441, 1635
SpeI ACTAGT 1 cut(s) 1093
SsiI CCGC 7 cut(s) 837, 972, 1065, 1071, 1172, 1425, 1427
SspI AATATT 1 cut(s) 1324
SspMI CTAG 5 cut(s) 774, 1056, 1094, 1818, 1961
StyD4I CCNGG 8 cut(s) 126, 172, 443, 487, 1162, 1258, 1288, 1336
StyI CCWWGG 1 cut(s) 724
TaaI ACNGT 4 cut(s) 599, 608, 1031, 1285
TaiI ACGT 4 cut(s) 164, 186, 1443, 1637
TatI WGTACW 4 cut(s) 198, 519, 857, 1839
TauI GCSGC 1 cut(s) 1430
TfiI GAWTC 4 cut(s) 192, 207, 232, 1749
Tru1I TTAA 5 cut(s) 318, 612, 962, 1245, 1314
Tru9I TTAA 5 cut(s) 318, 612, 962, 1245, 1314
TseFI GTSAC 1 cut(s) 1609
TseI GCWGC 2 cut(s) 430, 1157
Tsp45I GTSAC 1 cut(s) 1609
TspDTI ATGAA 6 cut(s) 419, 538, 1481, 1563, 1653, 1820
TspGWI ACGGA 1 cut(s) 43
Van91I CCANNNNNTGG 3 cut(s) 483, 1385, 1398
VpaK11BI GGWCC 4 cut(s) 721, 811, 1388, 1967
XapI RAATTY 5 cut(s) 351, 539, 1310, 1656, 1827
XhoI CTCGAG 1 cut(s) 381
XmnI GAANNNNTTC 2 cut(s) 633, 1050
XspI CTAG 5 cut(s) 774, 1056, 1094, 1818, 1961
ZraI GACGTC 1 cut(s) 184
ZrmI AGTACT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.