AT2G21655

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000474
Physical Location & Seq
Forward (+)
9263537 .. 9264098
562 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21655.1

Sequence Viewer

Length: 471 bp
ATGGAGAATAAAACAATGTTTATGATATTTTCTTTGATCATGGTTTTGCTATCGTTTTCTCATCCAACTTTCGGCAAAGAAAGCGATAATGACAAGCCACTCCTCATTTCAGACGACGAGTTTGATGCTATGATGGCAAGATCTCCTACATCGGACAATTATAACGAAAATGTTGGTAGTAAATATTCAAAGAAACAAATAAATTATCTTATGAACTGCAGTAAAAAGATGGCCGTCCCAGATAAATGTATAGAAGAAGTGATGGCTGAAATTATTCAGAACAAAAGTGCTTCAAGAGATTGTTGTTTGGGGATAGTGAAAGCTGGAAAGGAATGTCACATGGAATATATGAAATTATTTTTTCAAATGTATGAACTCAGGCGTTTTACTTCTAAAAGGTTTTCCAAAACTAATGAGATATGGAACAGATGTTCCACCGAAATTGGAGTTGTTTCACCATATTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

156

Amino Acids

18.02

Weight (kDa)

7.55

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 72 - 145 5.7e-18 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 162
AccB7I CCANNNNNTGG 1 cut(s) 464
AcoI YGGCCR 1 cut(s) 231
AfiI CCNNNNNNNGG 2 cut(s) 71, 464
AgsI TTSAA 3 cut(s) 189, 294, 365
AloI GAACNNNNNNTCC 4 cut(s) 415, 416, 447, 448
AluBI AGCT 1 cut(s) 323
AluI AGCT 1 cut(s) 323
AoxI GGCC 1 cut(s) 231
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
Asp700I GAANNNNTTC 1 cut(s) 273
AsuHPI GGTGA 1 cut(s) 447
BccI CCATC 3 cut(s) 127, 223, 256
BceAI ACGGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BclI TGATCA 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 217
BglII AGATCT 1 cut(s) 140
BmsI GCATC 1 cut(s) 115
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 464
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 2 cut(s) 71, 464
BseMII CTCAG 1 cut(s) 391
BseRI GAGGAG 1 cut(s) 92
BshFI GGCC 1 cut(s) 233
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 71, 464
BsmFI GGGAC 1 cut(s) 221
BsnI GGCC 1 cut(s) 233
Bsp143I GATC 2 cut(s) 36, 140
BspANI GGCC 1 cut(s) 233
BspCNI CTCAG 1 cut(s) 390
BspMAI CTGCAG 1 cut(s) 221
BssMI GATC 2 cut(s) 36, 140
BstDEI CTNAG 1 cut(s) 377
BstF5I GGATG 1 cut(s) 61
BstKTI GATC 2 cut(s) 39, 143
BstMBI GATC 2 cut(s) 36, 140
BstMWI GCNNNNNNNGC 2 cut(s) 81, 134
BstSFI CTRYAG 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
BsuRI GGCC 1 cut(s) 233
BtsCI GGATG 1 cut(s) 61
CspCI CAANNNNNGTGG 2 cut(s) 424, 459
CviAII CATG 2 cut(s) 40, 340
CviJI RGCY 4 cut(s) 97, 233, 266, 323
CviKI_1 RGCY 4 cut(s) 97, 233, 266, 323
DdeI CTNAG 1 cut(s) 377
DpnI GATC 2 cut(s) 38, 142
DpnII GATC 2 cut(s) 36, 140
EaeI YGGCCR 1 cut(s) 231
FaeI CATG 2 cut(s) 43, 343
FaqI GGGAC 1 cut(s) 221
FatI CATG 2 cut(s) 39, 339
FbaI TGATCA 1 cut(s) 36
FokI GGATG 1 cut(s) 48
HaeIII GGCC 1 cut(s) 233
Hin1II CATG 2 cut(s) 43, 343
HphI GGTGA 1 cut(s) 447
Hpy188I TCNGA 3 cut(s) 112, 154, 279
Hpy188III TCNNGA 1 cut(s) 294
Hpy99I CGWCG 1 cut(s) 119
HpyCH4V TGCA 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 134
HpyF3I CTNAG 1 cut(s) 377
Hsp92II CATG 2 cut(s) 43, 343
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 2 cut(s) 36, 140
LpnPI CCDG 4 cut(s) 252, 309, 364, 450
LweI GCATC 1 cut(s) 115
MaeIII GTNAC 1 cut(s) 335
MalI GATC 2 cut(s) 38, 142
MboI GATC 2 cut(s) 36, 140
MboII GAAGA 1 cut(s) 266
MflI RGATCY 1 cut(s) 140
MluCI AATT 5 cut(s) 157, 202, 270, 353, 441
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 1 cut(s) 113
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 1 cut(s) 469
MwoI GCNNNNNNNGC 2 cut(s) 81, 134
NdeII GATC 2 cut(s) 36, 140
NlaIII CATG 2 cut(s) 43, 343
NmuCI GTSAC 1 cut(s) 335
PdmI GAANNNNTTC 1 cut(s) 273
PflMI CCANNNNNTGG 1 cut(s) 464
PsiI TTATAA 1 cut(s) 162
PstI CTGCAG 1 cut(s) 221
PsuI RGATCY 1 cut(s) 140
SaqAI TTAA 1 cut(s) 469
Sau3AI GATC 2 cut(s) 36, 140
SetI ASST 2 cut(s) 325, 401
SfaNI GCATC 1 cut(s) 115
SfcI CTRYAG 1 cut(s) 217
SgeI CNNG 9 cut(s) 52, 106, 130, 150, 251, 306, 336, 352, 391
Sse9I AATT 5 cut(s) 157, 202, 270, 353, 441
SspI AATATT 1 cut(s) 185
TasI AATT 5 cut(s) 157, 202, 270, 353, 441
Tru1I TTAA 1 cut(s) 469
Tru9I TTAA 1 cut(s) 469
TseFI GTSAC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 3 cut(s) 227, 365, 387
Van91I CCANNNNNTGG 1 cut(s) 464
XmnI GAANNNNTTC 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.