Rh5BG500300

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000474
Physical Location & Seq
Forward (+)
79657026 .. 79657490
465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG500300.1

Sequence Viewer

Length: 465 bp
ATGGCTAGAATTGATCAAAATGTGTTTGTCAAGGTAGGTTTGGTGTTGGTAAGTGTAGCACTATTCATGTCATCATTTGCTGCAGCTCAAGAATATGAGGTTATTTATCAAGTTTCTGAGGATGTTGCGGCAAGCCCTATTGGCATCGAGGTTGACTATTTTGCCAGTAATGATGGAAACGGTCAATATGCGTTCCCTCCTATCCAATCTTATAAGAAGTTAGATGAATGTGTAAATAAGATTACAGGATATTATACTGAAGAGATCTTGCCCAAAATTTTTAAACGCGAACCTCTCACTACGGATAATTGCAAAGCCATTCTGCATTTGGGATATGATTGTCACATTCGGTCAGTGAAAACTCTGCTCTCACGTCCGGAACTAAAAGAAAAAGCTTCTGAGATACTGCCTAGAAGTGAGCAGATTTGGGAAACATGTGGCTTTGTTGCCCCTTCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

154

Amino Acids

17.3

Weight (kDa)

5.06

Isoelectric Point (pI)

65.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 76 - 146 1.2e-06 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 213
AccII CGCG 1 cut(s) 288
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 1 cut(s) 279
AflIII ACRYGT 1 cut(s) 434
AjiI CACGTC 1 cut(s) 374
AluBI AGCT 2 cut(s) 86, 395
AluI AGCT 2 cut(s) 86, 395
Aor13HI TCCGGA 1 cut(s) 376
ApeKI GCWGC 2 cut(s) 80, 83
ApoI RAATTY 1 cut(s) 276
BbvI GCAGC 2 cut(s) 67, 95
BccI CCATC 1 cut(s) 167
BclI TGATCA 1 cut(s) 13
BfaI CTAG 2 cut(s) 6, 411
BfmI CTRYAG 1 cut(s) 81
BglI GCCNNNNNGGC 1 cut(s) 141
BglII AGATCT 1 cut(s) 264
BisI GCNGC 3 cut(s) 81, 84, 129
BlsI GCNGC 3 cut(s) 82, 85, 130
BmgBI CACGTC 1 cut(s) 374
BmsI GCATC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 376
Bse1I ACTGG 1 cut(s) 165
BseAI TCCGGA 1 cut(s) 376
BseGI GGATG 1 cut(s) 127
BseMII CTCAG 2 cut(s) 108, 390
BseNI ACTGG 1 cut(s) 165
BseXI GCAGC 2 cut(s) 67, 95
Bsh1236I CGCG 1 cut(s) 288
BsiSI CCGG 1 cut(s) 377
Bsp13I TCCGGA 1 cut(s) 376
Bsp143I GATC 2 cut(s) 13, 264
BspACI CCGC 1 cut(s) 128
BspCNI CTCAG 2 cut(s) 109, 391
BspEI TCCGGA 1 cut(s) 376
BspFNI CGCG 1 cut(s) 288
BspMAI CTGCAG 1 cut(s) 85
BsrI ACTGG 1 cut(s) 165
BssMI GATC 2 cut(s) 13, 264
Bst4CI ACNGT 1 cut(s) 182
Bst6I CTCTTC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 133
BstDEI CTNAG 2 cut(s) 117, 399
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 1 cut(s) 288
BstKTI GATC 2 cut(s) 16, 267
BstMBI GATC 2 cut(s) 13, 264
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 438
BstSFI CTRYAG 1 cut(s) 81
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 2 cut(s) 67, 95
BstX2I RGATCY 1 cut(s) 264
BstYI RGATCY 1 cut(s) 264
BtrI CACGTC 1 cut(s) 374
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 133
CviAII CATG 2 cut(s) 67, 435
CviJI RGCY 6 cut(s) 5, 86, 135, 317, 395, 441
CviKI_1 RGCY 6 cut(s) 5, 86, 135, 317, 395, 441
DdeI CTNAG 2 cut(s) 117, 399
DpnI GATC 2 cut(s) 15, 266
DpnII GATC 2 cut(s) 13, 264
DraI TTTAAA 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco57I CTGAAG 1 cut(s) 279
FaeI CATG 2 cut(s) 70, 438
FaiI YATR 7 cut(s) 68, 96, 189, 213, 255, 336, 436
FatI CATG 2 cut(s) 66, 434
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 81, 84, 129
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 3 cut(s) 81, 84, 129
FspBI CTAG 2 cut(s) 6, 411
GluI GCNGC 3 cut(s) 81, 84, 129
HapII CCGG 1 cut(s) 377
Hin1II CATG 2 cut(s) 70, 438
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 393
HpaII CCGG 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 118, 400
Hpy188III TCNNGA 2 cut(s) 89, 377
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 1 cut(s) 462
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 3 cut(s) 83, 312, 325
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 2 cut(s) 117, 399
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 2 cut(s) 70, 438
Kpn2I TCCGGA 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 2 cut(s) 13, 264
LpnPI CCDG 3 cut(s) 178, 231, 390
Lsp1109I GCAGC 2 cut(s) 67, 95
LweI GCATC 1 cut(s) 153
MaeI CTAG 2 cut(s) 6, 411
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 341
MalI GATC 2 cut(s) 15, 266
MboI GATC 2 cut(s) 13, 264
MboII GAAGA 1 cut(s) 272
MflI RGATCY 1 cut(s) 264
MluCI AATT 3 cut(s) 9, 276, 307
MnlI CCTC 5 cut(s) 91, 112, 142, 207, 303
MroI TCCGGA 1 cut(s) 376
MseI TTAA 1 cut(s) 282
MspI CCGG 1 cut(s) 377
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 141
NdeII GATC 2 cut(s) 13, 264
NlaIII CATG 2 cut(s) 70, 438
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 1 cut(s) 438
PciI ACATGT 1 cut(s) 434
PkrI GCNGC 3 cut(s) 82, 85, 130
PscI ACATGT 1 cut(s) 434
PsiI TTATAA 1 cut(s) 213
PstI CTGCAG 1 cut(s) 85
PsuI RGATCY 1 cut(s) 264
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 3 cut(s) 81, 84, 129
Sau3AI GATC 2 cut(s) 13, 264
SetI ASST 8 cut(s) 36, 40, 88, 102, 153, 295, 376, 397
SfaNI GCATC 1 cut(s) 153
SfcI CTRYAG 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 3 cut(s) 9, 276, 307
SsiI CCGC 1 cut(s) 128
SspMI CTAG 2 cut(s) 6, 411
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 376
TaqI TCGA 1 cut(s) 147
TaqII GACCGA 1 cut(s) 339
TasI AATT 3 cut(s) 9, 276, 307
TauI GCSGC 1 cut(s) 131
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 360
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 2 cut(s) 80, 83
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 2 cut(s) 55, 240
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 360
XapI RAATTY 1 cut(s) 276
XceI RCATGY 1 cut(s) 438
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XspI CTAG 2 cut(s) 6, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.