Rh5BG501500

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000474
Physical Location & Seq
Forward (+)
79782645 .. 79783139
495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG501500.1

Sequence Viewer

Length: 495 bp
ATGGCTAGAGTTGATCAAAATGTGTTTGTCAAGGTAGGTTTGGTGTTAGTAAGTGTAGCACTATTCATGTCATCATTTACAGCAGCTCAAGAATATGAGGTAATTTATCAAGTTTCTAAGGATGCTACGGCGAGCCCTATTAGCATTGAGGTTGACTCTTTAGTCATTGATGTTGCCTTCCCTCCAATTTCTTTAGAAAACGGTCAATATCCGTTCCCTCCTAACGTATCACCGGAATCTGATATGAAGTTGGATGAATGTGTAAATAAGATTACAGGATATTATGCTGAAGAGATCTTGCCCAAAATTTTTAAACGCGAACCTCTCACTAAGGATAATTGCAAAGCCATTCTGCATTTGGGATATGATTGTCACATTCGGTCAGTGAAAACTCTGCTCTCACGTCCGGAACTAAAAGAAAAAGCTTCTGAGATACTGCCTAAAAGTGTGCAGATTTGGGAAACATGTGCCTTTGTTGCCCCTTCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

164

Amino Acids

18.3

Weight (kDa)

5.07

Isoelectric Point (pI)

62.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 86 - 156 1.3e-06 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccII CGCG 1 cut(s) 318
AccIII TCCGGA 1 cut(s) 406
AcsI RAATTY 1 cut(s) 306
AcuI CTGAAG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 464
AjiI CACGTC 1 cut(s) 404
AluBI AGCT 2 cut(s) 86, 425
AluI AGCT 2 cut(s) 86, 425
Aor13HI TCCGGA 1 cut(s) 406
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 306
AsuHPI GGTGA 1 cut(s) 222
BanII GRGCYC 1 cut(s) 137
BbvI GCAGC 1 cut(s) 95
BceAI ACGGC 1 cut(s) 144
BclI TGATCA 1 cut(s) 13
BfaI CTAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 294
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
BmgBI CACGTC 1 cut(s) 404
BmsI GCATC 1 cut(s) 112
BplI GAGNNNNNCTC 2 cut(s) 140, 172
BpuEI CTTGAG 1 cut(s) 72
BsaWI WCCGGW 2 cut(s) 232, 406
BseAI TCCGGA 1 cut(s) 406
BseGI GGATG 2 cut(s) 127, 259
BseMII CTCAG 1 cut(s) 420
BseXI GCAGC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 318
BsiSI CCGG 2 cut(s) 233, 407
Bsp1286I GDGCHC 1 cut(s) 137
Bsp13I TCCGGA 1 cut(s) 406
Bsp143I GATC 2 cut(s) 13, 294
BspCNI CTCAG 1 cut(s) 421
BspEI TCCGGA 1 cut(s) 406
BspFNI CGCG 1 cut(s) 318
BssMI GATC 2 cut(s) 13, 294
Bst4CI ACNGT 1 cut(s) 203
Bst6I CTCTTC 1 cut(s) 285
BstC8I GCNNGC 1 cut(s) 133
BstDEI CTNAG 3 cut(s) 117, 330, 429
BstF5I GGATG 2 cut(s) 127, 259
BstFNI CGCG 1 cut(s) 318
BstKTI GATC 2 cut(s) 16, 297
BstMBI GATC 2 cut(s) 13, 294
BstMWI GCNNNNNNNGC 2 cut(s) 141, 476
BstNSI RCATGY 1 cut(s) 468
BstUI CGCG 1 cut(s) 318
BstV1I GCAGC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 2 cut(s) 127, 259
BtsIMutI CAGTG 1 cut(s) 390
Cac8I GCNNGC 1 cut(s) 133
CviAII CATG 2 cut(s) 67, 465
CviJI RGCY 5 cut(s) 5, 86, 135, 347, 425
CviKI_1 RGCY 5 cut(s) 5, 86, 135, 347, 425
DdeI CTNAG 3 cut(s) 117, 330, 429
DpnI GATC 2 cut(s) 15, 296
DpnII GATC 2 cut(s) 13, 294
DraI TTTAAA 1 cut(s) 313
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 2 cut(s) 70, 468
FaiI YATR 6 cut(s) 68, 96, 245, 285, 366, 466
FatI CATG 2 cut(s) 66, 464
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 2 cut(s) 134, 266
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 1 cut(s) 6
GluI GCNGC 1 cut(s) 84
HapII CCGG 2 cut(s) 233, 407
Hin1II CATG 2 cut(s) 70, 468
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 423
HinfI GANTC 2 cut(s) 155, 236
HpaII CCGG 2 cut(s) 233, 407
HphI GGTGA 1 cut(s) 222
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 241, 430
Hpy188III TCNNGA 2 cut(s) 89, 407
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 187, 492
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4IV ACGT 2 cut(s) 225, 403
HpyCH4V TGCA 3 cut(s) 342, 355, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 476
HpyF3I CTNAG 3 cut(s) 117, 330, 429
HpySE526I ACGT 2 cut(s) 225, 403
Hsp92II CATG 2 cut(s) 70, 468
Kpn2I TCCGGA 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 2 cut(s) 13, 294
LpnPI CCDG 3 cut(s) 246, 261, 420
Lsp1109I GCAGC 1 cut(s) 95
LweI GCATC 1 cut(s) 112
MaeI CTAG 1 cut(s) 6
MaeII ACGT 2 cut(s) 225, 403
MaeIII GTNAC 1 cut(s) 371
MalI GATC 2 cut(s) 15, 296
MboI GATC 2 cut(s) 13, 294
MboII GAAGA 1 cut(s) 302
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 137
MluCI AATT 4 cut(s) 102, 186, 306, 337
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 5 cut(s) 91, 142, 192, 228, 333
MroI TCCGGA 1 cut(s) 406
MseI TTAA 1 cut(s) 312
MspI CCGG 2 cut(s) 233, 407
MvnI CGCG 1 cut(s) 318
MwoI GCNNNNNNNGC 2 cut(s) 141, 476
NdeII GATC 2 cut(s) 13, 294
NlaIII CATG 2 cut(s) 70, 468
NmuCI GTSAC 1 cut(s) 371
NspI RCATGY 1 cut(s) 468
PciI ACATGT 1 cut(s) 464
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
PscI ACATGT 1 cut(s) 464
PsuI RGATCY 1 cut(s) 294
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 13, 294
SchI GAGTC 1 cut(s) 149
SduI GDGCHC 1 cut(s) 137
SetI ASST 9 cut(s) 36, 40, 88, 102, 153, 228, 325, 406, 427
SfaNI GCATC 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 4 cut(s) 102, 186, 306, 337
SspMI CTAG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 203
TaiI ACGT 2 cut(s) 228, 406
TaqII GACCGA 1 cut(s) 369
TasI AATT 4 cut(s) 102, 186, 306, 337
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 1 cut(s) 390
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 3 cut(s) 55, 260, 270
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 1 cut(s) 390
XapI RAATTY 1 cut(s) 306
XceI RCATGY 1 cut(s) 468
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.