Rh5DG512400

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000474
Physical Location & Seq
Forward (+)
79909400 .. 79909945
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG512400.1

Sequence Viewer

Length: 546 bp
ATGGCTAGAGTTGATCAAAATGTGTTTGTCAAGGTAGGTTTGGTGTTAGTAAGTGTAGCACTATTCATGTCATCATTTGCAGGAGCTCAAGAATATGAGGTTATTTATCAAGTTTCTGAAGATGCTGCGGCGAGCCCTATTGGCATCGAGGTTGACTATTTAGTCATTGATGATGCCTTCCCTCCAATTTCTTCAGAAAATGGTCAATATACGTTCCCTTCTAACCAATCACCGGAATCTTATAAGAAGTTGGATGAATGTGTAAATAAGATTGCAGAATATTATGCTGAAGAGATCTTGGCCAGAATTTTTAAAAGCGAACCTCTCACTACGGATAATTGCAAAGCCCTTCTGCATTTGGGATATGATTGTCACATCCGGTCAGTGAAAACTCTGCTCTCACTTCCGGAACTAAAAGAAAAGGCTTCTGAGATACTGCCTAAAAGTGTGCAGATTTGGGAAACATGTGCTTTTGTTGCCCCTTCTCCTTTCTTATTGATCTTATTGCACACTAGCTCATGTAATCCATCCATACAAATTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

181

Amino Acids

20.05

Weight (kDa)

4.68

Isoelectric Point (pI)

62.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 86 - 156 1.6e-06 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 243
AasI GACNNNNNNGTC 1 cut(s) 161
AccIII TCCGGA 1 cut(s) 406
AciI CCGC 1 cut(s) 128
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 306
AcuI CTGAAG 3 cut(s) 138, 177, 309
AfiI CCNNNNNNNGG 1 cut(s) 232
AflIII ACRYGT 1 cut(s) 464
AluBI AGCT 2 cut(s) 86, 516
AluI AGCT 2 cut(s) 86, 516
Alw21I GWGCWC 1 cut(s) 88
Aor13HI TCCGGA 1 cut(s) 406
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 1 cut(s) 306
AsuHPI GGTGA 1 cut(s) 222
BalI TGGCCA 1 cut(s) 302
BanII GRGCYC 2 cut(s) 88, 137
Bbv12I GWGCWC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 112
BccI CCATC 1 cut(s) 535
BclI TGATCA 1 cut(s) 13
BfaI CTAG 2 cut(s) 6, 513
BglI GCCNNNNNGGC 1 cut(s) 141
BglII AGATCT 1 cut(s) 294
BisI GCNGC 2 cut(s) 126, 129
BlsI GCNGC 2 cut(s) 127, 130
BmsI GCATC 3 cut(s) 112, 153, 163
BpuEI CTTGAG 1 cut(s) 72
BsaWI WCCGGW 3 cut(s) 232, 378, 406
Bsc4I CCNNNNNNNGG 1 cut(s) 232
BseAI TCCGGA 1 cut(s) 406
BseGI GGATG 3 cut(s) 259, 375, 527
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 420
BseXI GCAGC 1 cut(s) 112
BsgI GTGCAG 1 cut(s) 470
BshFI GGCC 1 cut(s) 302
BsiHKAI GWGCWC 1 cut(s) 88
BsiSI CCGG 3 cut(s) 233, 379, 407
BslI CCNNNNNNNGG 1 cut(s) 232
BsnI GGCC 1 cut(s) 302
Bsp1286I GDGCHC 2 cut(s) 88, 137
Bsp13I TCCGGA 1 cut(s) 406
Bsp143I GATC 3 cut(s) 13, 294, 498
BspACI CCGC 1 cut(s) 128
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 1 cut(s) 421
BspEI TCCGGA 1 cut(s) 406
BssMI GATC 3 cut(s) 13, 294, 498
Bst6I CTCTTC 1 cut(s) 285
BstC8I GCNNGC 1 cut(s) 133
BstDEI CTNAG 1 cut(s) 429
BstF5I GGATG 3 cut(s) 259, 375, 527
BstKTI GATC 3 cut(s) 16, 297, 501
BstMBI GATC 3 cut(s) 13, 294, 498
BstMWI GCNNNNNNNGC 2 cut(s) 141, 476
BstNSI RCATGY 1 cut(s) 468
BstV1I GCAGC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuRI GGCC 1 cut(s) 302
BtsCI GGATG 3 cut(s) 259, 375, 527
BtsIMutI CAGTG 1 cut(s) 390
Cac8I GCNNGC 1 cut(s) 133
CviAII CATG 3 cut(s) 67, 465, 519
CviJI RGCY 7 cut(s) 5, 86, 135, 302, 347, 425, 516
CviKI_1 RGCY 7 cut(s) 5, 86, 135, 302, 347, 425, 516
DdeI CTNAG 1 cut(s) 429
DpnI GATC 3 cut(s) 15, 296, 500
DpnII GATC 3 cut(s) 13, 294, 498
DraI TTTAAA 1 cut(s) 313
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 86
Eco24I GRGCYC 2 cut(s) 88, 137
Eco53kI GAGCTC 1 cut(s) 86
Eco57I CTGAAG 3 cut(s) 138, 177, 309
EcoICRI GAGCTC 1 cut(s) 86
EcoT38I GRGCYC 2 cut(s) 88, 137
FaeI CATG 3 cut(s) 70, 468, 522
FaiI YATR 9 cut(s) 68, 96, 210, 243, 285, 366, 466, 520, 533
FatI CATG 3 cut(s) 66, 464, 518
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 2 cut(s) 126, 129
FokI GGATG 3 cut(s) 266, 362, 514
FriOI GRGCYC 2 cut(s) 88, 137
Fsp4HI GCNGC 2 cut(s) 126, 129
FspBI CTAG 2 cut(s) 6, 513
GluI GCNGC 2 cut(s) 126, 129
HaeIII GGCC 1 cut(s) 302
HapII CCGG 3 cut(s) 233, 379, 407
Hin1II CATG 3 cut(s) 70, 468, 522
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 1 cut(s) 236
HpaII CCGG 3 cut(s) 233, 379, 407
HphI GGTGA 1 cut(s) 222
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 3 cut(s) 118, 196, 430
Hpy188III TCNNGA 2 cut(s) 89, 407
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 4 cut(s) 187, 228, 359, 492
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 6 cut(s) 80, 275, 342, 355, 451, 508
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 476
HpyF3I CTNAG 1 cut(s) 429
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 3 cut(s) 70, 468, 522
Kpn2I TCCGGA 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 3 cut(s) 13, 294, 498
LmnI GCTCC 1 cut(s) 83
LpnPI CCDG 5 cut(s) 66, 246, 316, 392, 420
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 3 cut(s) 112, 153, 163
MaeI CTAG 2 cut(s) 6, 513
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 371
MalI GATC 3 cut(s) 15, 296, 500
MboI GATC 3 cut(s) 13, 294, 498
MboII GAAGA 3 cut(s) 131, 183, 302
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 2 cut(s) 88, 137
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 4 cut(s) 186, 306, 337, 537
MluNI TGGCCA 1 cut(s) 302
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 4 cut(s) 91, 142, 192, 333
Mox20I TGGCCA 1 cut(s) 302
MroI TCCGGA 1 cut(s) 406
MscI TGGCCA 1 cut(s) 302
MseI TTAA 2 cut(s) 312, 544
Msp20I TGGCCA 1 cut(s) 302
MspI CCGG 3 cut(s) 233, 379, 407
MwoI GCNNNNNNNGC 2 cut(s) 141, 476
NdeII GATC 3 cut(s) 13, 294, 498
NlaIII CATG 3 cut(s) 70, 468, 522
NmuCI GTSAC 1 cut(s) 371
NspI RCATGY 1 cut(s) 468
PciI ACATGT 1 cut(s) 464
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 2 cut(s) 127, 130
PscI ACATGT 1 cut(s) 464
PsiI TTATAA 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 88
PsuI RGATCY 1 cut(s) 294
SacI GAGCTC 1 cut(s) 88
SaqAI TTAA 2 cut(s) 312, 544
SatI GCNGC 2 cut(s) 126, 129
Sau3AI GATC 3 cut(s) 13, 294, 498
SduI GDGCHC 2 cut(s) 88, 137
SetI ASST 8 cut(s) 36, 40, 88, 102, 153, 215, 325, 518
SfaNI GCATC 3 cut(s) 112, 153, 163
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 4 cut(s) 186, 306, 337, 537
SsiI CCGC 1 cut(s) 128
SspI AATATT 1 cut(s) 281
SspMI CTAG 2 cut(s) 6, 513
SstI GAGCTC 1 cut(s) 88
TaiI ACGT 1 cut(s) 215
TaqI TCGA 1 cut(s) 147
TasI AATT 4 cut(s) 186, 306, 337, 537
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 2 cut(s) 312, 544
Tru9I TTAA 2 cut(s) 312, 544
TscAI CASTG 1 cut(s) 390
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 1 cut(s) 125
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 2 cut(s) 55, 270
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 1 cut(s) 390
XapI RAATTY 1 cut(s) 306
XceI RCATGY 1 cut(s) 468
XspI CTAG 2 cut(s) 6, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.