Rh5CG523200

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000474
Physical Location & Seq
Forward (+)
73348669 .. 73349142
474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG523200.1

Sequence Viewer

Length: 474 bp
ATGGCTAGAGTTGATCAAAATGTGTTTGTCAAGGTAGGTTTGGTGTTAGTAAGTGTAGCACTATTCATGTCATCATTTGTAGCAGCTCAAGAATATGAGGTTATTTATCAAGTTTCTGAGTATGCTGCGGCGAGCCCTATTGGCATTGAGGTTGACTATTTCGTCATTGATGATGAAAACGGTCAATATGCATTCCCTCCTTACCTATCACCGGAATCTTATAAGAAGTTGGATGAATGTGTAAATAAGATTACAGAATATTATGCTGAAGAGATCTTGGCCAGAATTTTTAAAAGCGAACCTCTCACTACGGATAATTGCAAAGCCCTTCTGCATTTGGGATATGATTGTCACATTCGGTCAATGAAAACTCTGCTCTCACGTCCGCAACTAAAAGAAAAGGCTTCTGAGATACTGCCTAAAAGTGTGCAGATTTGGGAAACATGTGCATTTGTTGCCCCTTCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

157

Amino Acids

17.78

Weight (kDa)

4.83

Isoelectric Point (pI)

62.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 79 - 149 4.5e-07 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AasI GACNNNNNNGTC 1 cut(s) 161
AciI CCGC 2 cut(s) 128, 386
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 285
AcuI CTGAAG 1 cut(s) 288
AfiI CCNNNNNNNGG 1 cut(s) 211
AflIII ACRYGT 1 cut(s) 443
AjiI CACGTC 1 cut(s) 383
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 2 cut(s) 83, 125
ApoI RAATTY 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 201
BalI TGGCCA 1 cut(s) 281
BanII GRGCYC 1 cut(s) 137
BbvI GCAGC 2 cut(s) 95, 112
BclI TGATCA 1 cut(s) 13
BfaI CTAG 1 cut(s) 6
BglI GCCNNNNNGGC 1 cut(s) 141
BglII AGATCT 1 cut(s) 273
BisI GCNGC 3 cut(s) 84, 126, 129
BlsI GCNGC 3 cut(s) 85, 127, 130
BmgBI CACGTC 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 1 cut(s) 211
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 108, 399
BseXI GCAGC 2 cut(s) 95, 112
BsgI GTGCAG 1 cut(s) 449
BshFI GGCC 1 cut(s) 281
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 211
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 137
Bsp143I GATC 2 cut(s) 13, 273
BspACI CCGC 2 cut(s) 128, 386
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 2 cut(s) 109, 400
BssMI GATC 2 cut(s) 13, 273
Bst4CI ACNGT 1 cut(s) 182
Bst6I CTCTTC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 133
BstDEI CTNAG 2 cut(s) 117, 408
BstF5I GGATG 1 cut(s) 238
BstKTI GATC 2 cut(s) 16, 276
BstMBI GATC 2 cut(s) 13, 273
BstMWI GCNNNNNNNGC 2 cut(s) 141, 455
BstNSI RCATGY 1 cut(s) 447
BstV1I GCAGC 2 cut(s) 95, 112
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 1 cut(s) 281
BtrI CACGTC 1 cut(s) 383
BtsCI GGATG 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 133
CviAII CATG 2 cut(s) 67, 444
CviJI RGCY 6 cut(s) 5, 86, 135, 281, 326, 404
CviKI_1 RGCY 6 cut(s) 5, 86, 135, 281, 326, 404
DdeI CTNAG 2 cut(s) 117, 408
DpnI GATC 2 cut(s) 15, 275
DpnII GATC 2 cut(s) 13, 273
DraI TTTAAA 1 cut(s) 292
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 2 cut(s) 70, 447
FaiI YATR 8 cut(s) 68, 96, 123, 189, 222, 264, 345, 445
FatI CATG 2 cut(s) 66, 443
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 84, 126, 129
FokI GGATG 1 cut(s) 245
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 3 cut(s) 84, 126, 129
FspBI CTAG 1 cut(s) 6
GluI GCNGC 3 cut(s) 84, 126, 129
HaeIII GGCC 1 cut(s) 281
HapII CCGG 1 cut(s) 212
Hin1II CATG 2 cut(s) 70, 447
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 1 cut(s) 215
HpaII CCGG 1 cut(s) 212
HphI GGTGA 1 cut(s) 201
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 118, 409
Hpy188III TCNNGA 1 cut(s) 89
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 338, 471
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 5 cut(s) 191, 321, 334, 430, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 455
HpyF3I CTNAG 2 cut(s) 117, 408
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 2 cut(s) 70, 447
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 2 cut(s) 13, 273
LpnPI CCDG 2 cut(s) 225, 295
Lsp1109I GCAGC 2 cut(s) 95, 112
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 1 cut(s) 350
MalI GATC 2 cut(s) 15, 275
MboI GATC 2 cut(s) 13, 273
MboII GAAGA 1 cut(s) 281
MflI RGATCY 1 cut(s) 273
MhlI GDGCHC 1 cut(s) 137
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 2 cut(s) 285, 316
MluNI TGGCCA 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 4 cut(s) 91, 142, 207, 312
Mox20I TGGCCA 1 cut(s) 281
Mph1103I ATGCAT 1 cut(s) 193
MscI TGGCCA 1 cut(s) 281
MseI TTAA 1 cut(s) 291
Msp20I TGGCCA 1 cut(s) 281
MspI CCGG 1 cut(s) 212
Mva1269I GAATGC 1 cut(s) 191
MwoI GCNNNNNNNGC 2 cut(s) 141, 455
NdeII GATC 2 cut(s) 13, 273
NlaIII CATG 2 cut(s) 70, 447
NmuCI GTSAC 1 cut(s) 350
NsiI ATGCAT 1 cut(s) 193
NspI RCATGY 1 cut(s) 447
PciI ACATGT 1 cut(s) 443
PctI GAATGC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 3 cut(s) 85, 127, 130
PscI ACATGT 1 cut(s) 443
PsiI TTATAA 1 cut(s) 222
PsuI RGATCY 1 cut(s) 273
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 3 cut(s) 84, 126, 129
Sau3AI GATC 2 cut(s) 13, 273
SduI GDGCHC 1 cut(s) 137
SetI ASST 8 cut(s) 36, 40, 88, 102, 153, 207, 304, 385
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 2 cut(s) 285, 316
SsiI CCGC 2 cut(s) 128, 386
SspI AATATT 1 cut(s) 260
SspMI CTAG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 385
TaqII GACCGA 1 cut(s) 348
TasI AATT 2 cut(s) 285, 316
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 1 cut(s) 215
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 2 cut(s) 83, 125
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 4 cut(s) 55, 189, 249, 380
TspGWI ACGGA 1 cut(s) 326
XapI RAATTY 1 cut(s) 285
XceI RCATGY 1 cut(s) 447
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.