RLG00000019311

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000474
Physical Location & Seq
Reverse (-)
50128413 .. 50128907
495 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019311

Sequence Viewer

Length: 495 bp
ATGGCAAGAGTTGATCAAAATGTGTTTGTCAAGGTGAGTTTGGTCTTAGCAAGTGTAGCATTATTCATGTCGTTTGCAGCAGCTCAAGACTTTCTTGCTTATGAAGTCTATAATGATGCCACAAGCCCTACAAGCGTTGAGGTTGAAGTCATTGATGCCTACCCTCCAATTTCTCCAGAAGACCATGATGCTCTCCCTCCACAACCATCACCGGGAGCTTACAAGAAGTTGAACAATTGTGCAAACAAGATTACAGCAGATTATGCCGAAGAGATCTACGATGGTATTTTCGAAGGCGAGCCTCTCACCACAGAGTGCTGCAAAGCCCTTGTGCATCTGGGATATGATTGTCACATCCAGTTAGTGAAATTTTTGCTCTCGAGTCCAGAACTTAAAGAAAAAGCTTCCGAGGTATTGCCTAGGAGTGTGCAGCTTTGGAAAACATGTGCCTTGGTGGTTGACGTTGATCAATCTATTGCTCCTTCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

165

Amino Acids

17.98

Weight (kDa)

4.46

Isoelectric Point (pI)

61.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 79 - 149 3.5e-11 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 368
AdeI CACNNNGTG 1 cut(s) 315
AfiI CCNNNNNNNGG 1 cut(s) 212
AflIII ACRYGT 1 cut(s) 443
AgsI TTSAA 2 cut(s) 146, 232
AluBI AGCT 4 cut(s) 83, 218, 404, 433
AluI AGCT 4 cut(s) 83, 218, 404, 433
Ama87I CYCGRG 1 cut(s) 379
ApeKI GCWGC 4 cut(s) 77, 80, 318, 430
ApoI RAATTY 1 cut(s) 368
AspA2I CCTAGG 1 cut(s) 419
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 3 cut(s) 46, 201, 298
AsuII TTCGAA 1 cut(s) 291
AvaI CYCGRG 1 cut(s) 379
AvrII CCTAGG 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 186
BbvI GCAGC 4 cut(s) 89, 92, 305, 442
BccI CCATC 2 cut(s) 214, 275
BclI TGATCA 2 cut(s) 13, 466
BcnI CCSGG 1 cut(s) 213
BfaI CTAG 1 cut(s) 420
BglII AGATCT 1 cut(s) 273
BisI GCNGC 4 cut(s) 78, 81, 319, 431
BlnI CCTAGG 1 cut(s) 419
BlsI GCNGC 4 cut(s) 79, 82, 320, 432
Bme1390I CCNGG 1 cut(s) 213
BmeT110I CYCGRG 1 cut(s) 379
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 4 cut(s) 106, 145, 178, 343
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 159
Bpu14I TTCGAA 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 408, 419, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 358
BseDI CCNNGG 3 cut(s) 408, 419, 450
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 1 cut(s) 212
BseNI ACTGG 1 cut(s) 358
BseXI GCAGC 4 cut(s) 89, 92, 305, 442
BsgI GTGCAG 1 cut(s) 449
BsiHKCI CYCGRG 1 cut(s) 379
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 379
Bsp119I TTCGAA 1 cut(s) 291
Bsp143I GATC 3 cut(s) 13, 273, 466
BspT104I TTCGAA 1 cut(s) 291
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 3 cut(s) 408, 419, 450
BssMI GATC 3 cut(s) 13, 273, 466
BssT1I CCWWGG 2 cut(s) 419, 450
Bst6I CTCTTC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 1 cut(s) 46
BstF5I GGATG 1 cut(s) 354
BstKTI GATC 3 cut(s) 16, 276, 469
BstMBI GATC 3 cut(s) 13, 273, 466
BstMWI GCNNNNNNNGC 3 cut(s) 56, 132, 263
BstNSI RCATGY 1 cut(s) 447
BstSCI CCNGG 1 cut(s) 211
BstV1I GCAGC 4 cut(s) 89, 92, 305, 442
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BtsCI GGATG 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 299
CviAII CATG 3 cut(s) 67, 185, 444
CviJI RGCY 7 cut(s) 83, 126, 218, 301, 326, 404, 433
CviKI_1 RGCY 7 cut(s) 83, 126, 218, 301, 326, 404, 433
DdeI CTNAG 1 cut(s) 46
DpnI GATC 3 cut(s) 15, 275, 468
DpnII GATC 3 cut(s) 13, 273, 466
DraIII CACNNNGTG 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 2 cut(s) 419, 450
Eco88I CYCGRG 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 419, 450
ErhI CCWWGG 2 cut(s) 419, 450
FaeI CATG 3 cut(s) 70, 188, 447
FaiI YATR 7 cut(s) 68, 102, 111, 186, 264, 345, 445
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FatI CATG 3 cut(s) 66, 184, 443
FbaI TGATCA 2 cut(s) 13, 466
Fnu4HI GCNGC 4 cut(s) 78, 81, 319, 431
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 4 cut(s) 78, 81, 319, 431
FspBI CTAG 1 cut(s) 420
GluI GCNGC 4 cut(s) 78, 81, 319, 431
GsuI CTGGAG 1 cut(s) 159
HapII CCGG 1 cut(s) 212
Hin1II CATG 3 cut(s) 70, 188, 447
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 402
HinfI GANTC 1 cut(s) 382
HpaII CCGG 1 cut(s) 212
HphI GGTGA 3 cut(s) 46, 201, 298
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 4 cut(s) 86, 176, 379, 386
Hpy8I GTNNAC 1 cut(s) 460
HpyAV CCTTC 2 cut(s) 287, 492
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 5 cut(s) 77, 242, 321, 334, 430
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 132, 263
HpyF3I CTNAG 1 cut(s) 46
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 3 cut(s) 70, 188, 447
Ksp22I TGATCA 2 cut(s) 13, 466
Kzo9I GATC 3 cut(s) 13, 273, 466
LmnI GCTCC 2 cut(s) 215, 484
LpnPI CCDG 5 cut(s) 189, 225, 323, 371, 399
Lsp1109I GCAGC 4 cut(s) 89, 92, 305, 442
LweI GCATC 4 cut(s) 106, 145, 178, 343
MaeI CTAG 1 cut(s) 420
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 1 cut(s) 350
MalI GATC 3 cut(s) 15, 275, 468
MboI GATC 3 cut(s) 13, 273, 466
MboII GAAGA 2 cut(s) 191, 281
MfeI CAATTG 1 cut(s) 235
MflI RGATCY 1 cut(s) 273
MluCI AATT 3 cut(s) 168, 235, 368
MlyI GAGTC 1 cut(s) 391
MnlI CCTC 5 cut(s) 133, 174, 207, 312, 403
MseI TTAA 1 cut(s) 393
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 1 cut(s) 235
MwoI GCNNNNNNNGC 3 cut(s) 56, 132, 263
NciI CCSGG 1 cut(s) 213
NdeII GATC 3 cut(s) 13, 273, 466
NlaIII CATG 3 cut(s) 70, 188, 447
NmuCI GTSAC 1 cut(s) 350
NspI RCATGY 1 cut(s) 447
NspV TTCGAA 1 cut(s) 291
PaeR7I CTCGAG 1 cut(s) 379
PciI ACATGT 1 cut(s) 443
PkrI GCNGC 4 cut(s) 79, 82, 320, 432
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
PscI ACATGT 1 cut(s) 443
PsuI RGATCY 1 cut(s) 273
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 4 cut(s) 78, 81, 319, 431
Sau3AI GATC 3 cut(s) 13, 273, 466
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 1 cut(s) 213
SetI ASST 8 cut(s) 36, 85, 144, 220, 406, 414, 435, 465
SfaNI GCATC 4 cut(s) 106, 145, 178, 343
Sfr274I CTCGAG 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 379
SmlI CTYRAG 2 cut(s) 84, 379
SmoI CTYRAG 2 cut(s) 84, 379
Sse9I AATT 3 cut(s) 168, 235, 368
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 2 cut(s) 419, 450
TaiI ACGT 1 cut(s) 465
TaqI TCGA 2 cut(s) 291, 380
TasI AATT 3 cut(s) 168, 235, 368
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 4 cut(s) 77, 80, 318, 430
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 2 cut(s) 55, 117
XapI RAATTY 1 cut(s) 368
XceI RCATGY 1 cut(s) 447
XhoI CTCGAG 1 cut(s) 379
XmaJI CCTAGG 1 cut(s) 419
XspI CTAG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.