Rorug05G0597400

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000474
Physical Location & Seq
Forward (+)
79512221 .. 79514170
1950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0597400.1

Sequence Viewer

Length: 753 bp
ATGGTGGAGGAGGAGCCGACGCCGACGCCGTCACCGGAAGAGAAGCAGGTGTTAGGCGATGACGGCGCGTATAAGGGAGTGGCAGCAGAAGCAGAAGCAGCAGCAGCTTCAATGGCTTCGATGGCGGAGGATCTGCAGCGTACGGTGATGCAATCGAGGGACTCTGCCATTCGTTCAGCTCGCACTCTCCAAGATAATGTGCCGGAATATATGGACAAAGCCGTCTCCGACTACAGGACCTACGAACATGCTTTCTTCCACAAAATCAAAGATGGATTGAAGAGTGCAACAGAGAACCCAGCCTCAACCATTGGAATTGGCCTCACTGCTGCTTTCATTCTTTTGCCAGGACCAAGAAGGTTCTTATTTCGCCATACATTGGGTCGATTTCAGAGCCTCGAGGCAAAATTTGCCAAAGCTGAGAAGAATGTAAACCAGTTCAAACTTTCAGTTGATCTGATGAAGCAGGAGAGCAAGAAACTTCTTGAGAGGGCAGCTTTTGCTGAAAAGGAAATGAAATTTGGCCAAACTGAGCTCAAGGATGTGGGAAGTCGAATTCAATCTCTATCCAAATCTATTCGCAAGGTTGACGCCCAAGCTGCAGATTTAATGGATGGGCTGCGAGAAATTCCTAGCGGGGAAGCTTTGAAGCTCAGAGCACAGGTTGCTGCAATGACATCACAATTGAAAACGCAGAAGTCTGTGATGGACAAACGCATAATGAAGATTTCGGAGTTAGGAGTTCCAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

250

Amino Acids

27.61

Weight (kDa)

8.9

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 37
AasI GACNNNNNNGTC 1 cut(s) 221
AbsI CCTCGAGG 1 cut(s) 398
Acc36I ACCTGC 1 cut(s) 37
AccB7I CCANNNNNTGG 1 cut(s) 379
AccII CGCG 1 cut(s) 68
AciI CCGC 2 cut(s) 125, 636
AclWI GGATC 1 cut(s) 138
AcoI YGGCCR 1 cut(s) 523
AcsI RAATTY 4 cut(s) 407, 518, 555, 627
AcyI GRCGYC 3 cut(s) 20, 26, 591
AfaI GTAC 1 cut(s) 142
AfiI CCNNNNNNNGG 2 cut(s) 234, 379
AgsI TTSAA 6 cut(s) 111, 280, 442, 560, 649, 688
AjnI CCWGG 1 cut(s) 346
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
AluBI AGCT 8 cut(s) 107, 179, 419, 497, 535, 599, 644, 652
AluI AGCT 8 cut(s) 107, 179, 419, 497, 535, 599, 644, 652
Alw21I GWGCWC 2 cut(s) 537, 661
Alw26I GTCTC 1 cut(s) 229
AlwI GGATC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 2 cut(s) 319, 523
ApoI RAATTY 4 cut(s) 407, 518, 555, 627
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 2 cut(s) 237, 350
AsuHPI GGTGA 2 cut(s) 24, 157
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 2 cut(s) 237, 350
BalI TGGCCA 1 cut(s) 525
BanII GRGCYC 1 cut(s) 537
Bbv12I GWGCWC 2 cut(s) 537, 661
BccI CCATC 4 cut(s) 115, 266, 608, 700
BceAI ACGGC 3 cut(s) 13, 79, 206
BciT130I CCWGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 229
BfaI CTAG 1 cut(s) 633
BfmI CTRYAG 3 cut(s) 134, 232, 600
BfuAI ACCTGC 1 cut(s) 37
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 2 cut(s) 237, 350
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 2 cut(s) 237, 350
BmiI GGNNCC 1 cut(s) 15
BmrFI CCNGG 1 cut(s) 348
BmsI GCATC 1 cut(s) 138
BpuEI CTTGAG 2 cut(s) 506, 521
BsaHI GRCGYC 3 cut(s) 20, 26, 591
BsaWI WCCGGW 1 cut(s) 34
BsaXI ACNNNNNCTCC 1 cut(s) 732
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 379
Bse1I ACTGG 2 cut(s) 436, 746
Bse3DI GCAATG 1 cut(s) 678
BseBI CCWGG 1 cut(s) 348
BseGI GGATG 2 cut(s) 547, 619
BseLI CCNNNNNNNGG 2 cut(s) 234, 379
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 3 cut(s) 411, 522, 667
BseNI ACTGG 2 cut(s) 436, 746
BseRI GAGGAG 2 cut(s) 23, 26
BseYI CCCAGC 1 cut(s) 298
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 2 cut(s) 321, 525
BsiHKAI GWGCWC 2 cut(s) 537, 661
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 2 cut(s) 35, 203
BsiWI CGTACG 1 cut(s) 140
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 2 cut(s) 234, 379
BsmAI GTCTC 1 cut(s) 229
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 2 cut(s) 321, 525
BsoBI CYCGRG 1 cut(s) 398
Bsp1286I GDGCHC 2 cut(s) 537, 661
Bsp143I GATC 2 cut(s) 130, 454
BspACI CCGC 2 cut(s) 125, 636
BspANI GGCC 2 cut(s) 321, 525
BspCNI CTCAG 3 cut(s) 412, 523, 666
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 1 cut(s) 15
BspMAI CTGCAG 2 cut(s) 138, 604
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 678
BsrI ACTGG 2 cut(s) 436, 746
BssMI GATC 2 cut(s) 130, 454
BssNI GRCGYC 3 cut(s) 20, 26, 591
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 2 cut(s) 33, 275
BstACI GRCGYC 3 cut(s) 20, 26, 591
BstAPI GCANNNNNTGC 3 cut(s) 410, 500, 665
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 3 cut(s) 420, 531, 653
BstF5I GGATG 2 cut(s) 547, 619
BstFNI CGCG 1 cut(s) 68
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 2 cut(s) 133, 457
BstMAI GTCTC 1 cut(s) 229
BstMBI GATC 2 cut(s) 130, 454
BstNI CCWGG 1 cut(s) 348
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 346
BstSFI CTRYAG 3 cut(s) 134, 232, 600
BstUI CGCG 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
BsuRI GGCC 2 cut(s) 321, 525
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 547, 619
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 2 cut(s) 324, 753
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 1 cut(s) 181
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 237, 350
CseI GACGC 3 cut(s) 28, 34, 599
Csp6I GTAC 1 cut(s) 141
CviAII CATG 1 cut(s) 248
CviQI GTAC 1 cut(s) 141
DdeI CTNAG 3 cut(s) 420, 531, 653
DpnI GATC 2 cut(s) 132, 456
DpnII GATC 2 cut(s) 130, 454
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 2 cut(s) 33, 275
EarI CTCTTC 2 cut(s) 33, 275
EciI GGCGGA 1 cut(s) 140
Ecl136II GAGCTC 1 cut(s) 535
Eco24I GRGCYC 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 237, 350
Eco53kI GAGCTC 1 cut(s) 535
Eco88I CYCGRG 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 535
EcoO109I RGGNCCY 1 cut(s) 237
EcoRI GAATTC 1 cut(s) 555
EcoRII CCWGG 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 537
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 1 cut(s) 251
FaiI YATR 6 cut(s) 72, 210, 212, 249, 375, 719
FaqI GGGAC 1 cut(s) 173
FatI CATG 1 cut(s) 247
FauI CCCGC 1 cut(s) 629
FokI GGATG 2 cut(s) 554, 626
FriOI GRGCYC 1 cut(s) 537
FspBI CTAG 1 cut(s) 633
GlaI GCGC 1 cut(s) 67
GsaI CCCAGC 1 cut(s) 302
HaeIII GGCC 2 cut(s) 321, 525
HapII CCGG 2 cut(s) 35, 203
HgaI GACGC 3 cut(s) 28, 34, 599
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 3 cut(s) 20, 26, 591
Hin1II CATG 1 cut(s) 251
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 642
HinfI GANTC 1 cut(s) 161
HpaII CCGG 2 cut(s) 35, 203
HphI GGTGA 2 cut(s) 24, 157
Hpy166II GTNNAC 2 cut(s) 433, 589
Hpy188I TCNGA 5 cut(s) 229, 393, 459, 656, 733
Hpy188III TCNNGA 1 cut(s) 485
Hpy8I GTNNAC 2 cut(s) 433, 589
Hpy99I CGWCG 2 cut(s) 22, 28
HpyAV CCTTC 1 cut(s) 351
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4V TGCA 5 cut(s) 136, 151, 287, 602, 671
HpyF3I CTNAG 3 cut(s) 420, 531, 653
Hsp92I GRCGYC 3 cut(s) 20, 26, 591
Hsp92II CATG 1 cut(s) 251
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 2 cut(s) 130, 454
LmnI GCTCC 1 cut(s) 13
LweI GCATC 1 cut(s) 138
MaeI CTAG 1 cut(s) 633
MaeIII GTNAC 1 cut(s) 30
MalI GATC 2 cut(s) 132, 456
MboI GATC 2 cut(s) 130, 454
MboII GAAGA 5 cut(s) 50, 247, 292, 436, 736
MfeI CAATTG 1 cut(s) 683
MflI RGATCY 1 cut(s) 130
MhlI GDGCHC 2 cut(s) 537, 661
MlsI TGGCCA 1 cut(s) 525
MluCI AATT 6 cut(s) 315, 407, 518, 555, 627, 683
MluNI TGGCCA 1 cut(s) 525
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 8 cut(s) 4, 121, 150, 313, 332, 394, 407, 483
Mox20I TGGCCA 1 cut(s) 525
MscI TGGCCA 1 cut(s) 525
MseI TTAA 1 cut(s) 608
Msp20I TGGCCA 1 cut(s) 525
MspI CCGG 2 cut(s) 35, 203
MspR9I CCNGG 1 cut(s) 348
MunI CAATTG 1 cut(s) 683
MvaI CCWGG 1 cut(s) 348
MvnI CGCG 1 cut(s) 68
NdeII GATC 2 cut(s) 130, 454
NlaIII CATG 1 cut(s) 251
NlaIV GGNNCC 1 cut(s) 15
NmuCI GTSAC 1 cut(s) 30
NspI RCATGY 1 cut(s) 251
PaeR7I CTCGAG 1 cut(s) 398
PaqCI CACCTGC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 1 cut(s) 26
Pfl23II CGTACG 1 cut(s) 140
PflFI GACNNNGTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 379
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PpuMI RGGWCCY 1 cut(s) 237
Psp124BI GAGCTC 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 346
PspFI CCCAGC 1 cut(s) 298
PspGI CCWGG 1 cut(s) 346
PspLI CGTACG 1 cut(s) 140
PspN4I GGNNCC 1 cut(s) 15
PspPI GGNCC 2 cut(s) 237, 350
PspPPI RGGWCCY 1 cut(s) 237
PspXI VCTCGAGB 1 cut(s) 398
PstI CTGCAG 2 cut(s) 138, 604
PsuI RGATCY 1 cut(s) 130
PsyI GACNNNGTC 1 cut(s) 28
RsaI GTAC 1 cut(s) 142
RsaNI GTAC 1 cut(s) 141
SacI GAGCTC 1 cut(s) 537
SaqAI TTAA 1 cut(s) 608
Sau3AI GATC 2 cut(s) 130, 454
Sau96I GGNCC 2 cut(s) 237, 350
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 2 cut(s) 537, 661
SfaNI GCATC 1 cut(s) 138
SfcI CTRYAG 3 cut(s) 134, 232, 600
Sfr274I CTCGAG 1 cut(s) 398
SinI GGWCC 2 cut(s) 237, 350
SlaI CTCGAG 1 cut(s) 398
SmlI CTYRAG 3 cut(s) 398, 485, 536
SmoI CTYRAG 3 cut(s) 398, 485, 536
Sse9I AATT 6 cut(s) 315, 407, 518, 555, 627, 683
SsiI CCGC 2 cut(s) 125, 636
SspMI CTAG 1 cut(s) 633
SstI GAGCTC 1 cut(s) 537
StyD4I CCNGG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 145
TaqI TCGA 5 cut(s) 119, 155, 385, 399, 553
TasI AATT 6 cut(s) 315, 407, 518, 555, 627, 683
Tru1I TTAA 1 cut(s) 608
Tru9I TTAA 1 cut(s) 608
TscAI CASTG 2 cut(s) 331, 753
TseFI GTSAC 1 cut(s) 30
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 4 cut(s) 325, 476, 530, 737
TspRI CASTG 2 cut(s) 331, 753
Tth111I GACNNNGTC 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 379
VpaK11BI GGWCC 2 cut(s) 237, 350
XapI RAATTY 4 cut(s) 407, 518, 555, 627
XceI RCATGY 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 398
XspI CTAG 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.