Rorug05G0422600

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000474
Physical Location & Seq
Reverse (-)
58725421 .. 58726251
831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0422600.1

Sequence Viewer

Length: 411 bp
ATGAAGTTCTGTCGATTTCTCCCGGAGACGAGGACGCGATTAAGTGCAAAGAGGCGCTTTCCGTTATTCGATTTTGCAAAAATCGTGCTCTGGATTTCAGCTTCTTCAAGTGCGGACGGCGCTGCTGCAGCTCGGCTCGGGGCGCGACGGAGCGGCGGGGGTTGCCGGAAGGACGCCAGGGGTCGGGCGGAGAGGTCTGCAGTCGGTGGAGTCGCCGGCGACGTCGTTGCAGCACTGCCCAGAAAACCTGCAGTTGCAGGTCGGGTTGCTGCCCAGAACCTGCAACTCGGACCTCCTCCGACCTCGAACGCTGCCCAGACCCGTCCGCCAAGCCTCCGACCTCCGTCCGCCAAGCCCCGAGCCGCCGTCGCCGCCTGGAAAGCCTCCGCTGCGTCGCCGTCCGCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

136

Amino Acids

14.06

Weight (kDa)

11.96

Isoelectric Point (pI)

55.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 3 cut(s) 248, 256, 288
AccBSI CCGCTC 1 cut(s) 153
AccII CGCG 2 cut(s) 37, 145
AcyI GRCGYC 2 cut(s) 174, 222
AfiI CCNNNNNNNGG 1 cut(s) 183
AgsI TTSAA 1 cut(s) 108
AjnI CCWGG 2 cut(s) 176, 374
AluBI AGCT 2 cut(s) 101, 131
AluI AGCT 2 cut(s) 101, 131
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 1 cut(s) 20
AlwNI CAGNNNCTG 1 cut(s) 280
Ama87I CYCGRG 2 cut(s) 137, 357
ApeKI GCWGC 7 cut(s) 122, 125, 128, 230, 269, 311, 389
AspLEI GCGC 3 cut(s) 57, 122, 145
AspS9I GGNCC 1 cut(s) 290
AsuC2I CCSGG 1 cut(s) 23
AvaI CYCGRG 2 cut(s) 137, 357
AvaII GGWCC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 7 cut(s) 109, 112, 140, 242, 256, 298, 376
BceAI ACGGC 3 cut(s) 133, 350, 382
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 2 cut(s) 178, 376
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 1 cut(s) 20
BfmI CTRYAG 3 cut(s) 126, 198, 249
BfoI RGCGCY 2 cut(s) 58, 123
BfuAI ACCTGC 3 cut(s) 248, 256, 288
Bme1390I CCNGG 3 cut(s) 23, 178, 376
Bme18I GGWCC 1 cut(s) 290
BmeT110I CYCGRG 2 cut(s) 137, 357
BmgT120I GGNCC 1 cut(s) 290
BmrFI CCNGG 3 cut(s) 23, 178, 376
BoxI GACNNNNGTC 1 cut(s) 343
BpuMI CCSGG 1 cut(s) 23
BsaHI GRCGYC 2 cut(s) 174, 222
BsaJI CCNNGG 1 cut(s) 177
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse118I RCCGGY 1 cut(s) 215
BseBI CCWGG 2 cut(s) 178, 376
BseDI CCNNGG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 183
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 7 cut(s) 109, 112, 140, 242, 256, 298, 376
Bsh1236I CGCG 2 cut(s) 37, 145
BsiHKAI GWGCWC 1 cut(s) 90
BsiHKCI CYCGRG 2 cut(s) 137, 357
BsiSI CCGG 3 cut(s) 23, 166, 216
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 20
BsmBI CGTCTC 1 cut(s) 20
BsoBI CYCGRG 2 cut(s) 137, 357
Bsp1286I GDGCHC 1 cut(s) 90
BspFNI CGCG 2 cut(s) 37, 145
BspMAI CTGCAG 3 cut(s) 130, 202, 253
BspMI ACCTGC 3 cut(s) 248, 256, 288
BsrBI CCGCTC 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 215
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 1 cut(s) 177
BssNI GRCGYC 2 cut(s) 174, 222
Bst2UI CCWGG 2 cut(s) 178, 376
BstACI GRCGYC 2 cut(s) 174, 222
BstC8I GCNNGC 1 cut(s) 217
BstFNI CGCG 2 cut(s) 37, 145
BstH2I RGCGCY 2 cut(s) 58, 123
BstHHI GCGC 3 cut(s) 57, 122, 145
BstMAI GTCTC 1 cut(s) 20
BstMWI GCNNNNNNNGC 8 cut(s) 119, 128, 142, 162, 368, 371, 380, 389
BstNI CCWGG 2 cut(s) 178, 376
BstPAI GACNNNNGTC 1 cut(s) 343
BstSCI CCNGG 3 cut(s) 21, 176, 374
BstSFI CTRYAG 3 cut(s) 126, 198, 249
BstUI CGCG 2 cut(s) 37, 145
BstV1I GCAGC 7 cut(s) 109, 112, 140, 242, 256, 298, 376
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 1 cut(s) 233
BveI ACCTGC 3 cut(s) 248, 256, 288
Cac8I GCNNGC 1 cut(s) 217
CaiI CAGNNNCTG 1 cut(s) 280
CfoI GCGC 3 cut(s) 57, 122, 145
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 290
CseI GACGC 3 cut(s) 43, 182, 381
CviJI RGCY 7 cut(s) 101, 131, 136, 333, 355, 362, 383
CviKI_1 RGCY 7 cut(s) 101, 131, 136, 333, 355, 362, 383
EciI GGCGGA 3 cut(s) 203, 315, 337
Eco47I GGWCC 1 cut(s) 290
Eco88I CYCGRG 2 cut(s) 137, 357
EcoRII CCWGG 2 cut(s) 176, 374
Esp3I CGTCTC 1 cut(s) 20
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FauI CCCGC 1 cut(s) 149
GlaI GCGC 3 cut(s) 56, 121, 144
HaeII RGCGCY 2 cut(s) 58, 123
HapII CCGG 3 cut(s) 23, 166, 216
HgaI GACGC 3 cut(s) 43, 182, 381
HhaI GCGC 3 cut(s) 57, 122, 145
Hin1I GRCGYC 2 cut(s) 174, 222
Hin6I GCGC 3 cut(s) 55, 120, 143
HinP1I GCGC 3 cut(s) 55, 120, 143
HinfI GANTC 1 cut(s) 210
HpaII CCGG 3 cut(s) 23, 166, 216
Hpy188I TCNGA 3 cut(s) 290, 300, 338
Hpy188III TCNNGA 1 cut(s) 91
Hpy99I CGWCG 5 cut(s) 150, 224, 227, 371, 397
HpyAV CCTTC 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 8 cut(s) 47, 77, 128, 200, 230, 251, 257, 283
HpyF10VI GCNNNNNNNGC 8 cut(s) 119, 128, 142, 162, 368, 371, 380, 389
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 2 cut(s) 174, 222
HspAI GCGC 3 cut(s) 55, 120, 143
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
LmnI GCTCC 1 cut(s) 150
Lsp1109I GCAGC 7 cut(s) 109, 112, 140, 242, 256, 298, 376
MaeII ACGT 1 cut(s) 222
MbiI CCGCTC 1 cut(s) 153
MboII GAAGA 1 cut(s) 96
MhlI GDGCHC 1 cut(s) 90
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 2 cut(s) 323, 361
MnlI CCTC 9 cut(s) 24, 45, 186, 303, 306, 313, 344, 351, 394
MreI CGCCGGCG 1 cut(s) 215
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 3 cut(s) 23, 166, 216
MspR9I CCNGG 3 cut(s) 23, 178, 376
MvaI CCWGG 2 cut(s) 178, 376
MvnI CGCG 2 cut(s) 37, 145
MwoI GCNNNNNNNGC 8 cut(s) 119, 128, 142, 162, 368, 371, 380, 389
NaeI GCCGGC 1 cut(s) 217
NciI CCSGG 1 cut(s) 23
NgoMIV GCCGGC 1 cut(s) 215
NmeAIII GCCGAG 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 21
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PshAI GACNNNNGTC 1 cut(s) 343
Psp6I CCWGG 2 cut(s) 176, 374
PspGI CCWGG 2 cut(s) 176, 374
PspPI GGNCC 1 cut(s) 290
PstI CTGCAG 3 cut(s) 130, 202, 253
PstNI CAGNNNCTG 1 cut(s) 280
SaqAI TTAA 1 cut(s) 41
Sau96I GGNCC 1 cut(s) 290
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 3 cut(s) 23, 178, 376
SduI GDGCHC 1 cut(s) 90
SfcI CTRYAG 3 cut(s) 126, 198, 249
SgrAI CRCCGGYG 1 cut(s) 215
SinI GGWCC 1 cut(s) 290
StyD4I CCNGG 3 cut(s) 21, 176, 374
TaiI ACGT 1 cut(s) 225
TaqI TCGA 3 cut(s) 13, 69, 305
TauI GCSGC 3 cut(s) 156, 365, 374
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TscAI CASTG 1 cut(s) 240
TseI GCWGC 7 cut(s) 122, 125, 128, 230, 269, 311, 389
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 51, 163, 333
TspRI CASTG 1 cut(s) 240
VpaK11BI GGWCC 1 cut(s) 290
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.