RchiOBHm_Chr5g0073331

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000474
Physical Location & Seq
Reverse (-)
79360548 .. 79361012
465 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34823

Sequence Viewer

Length: 465 bp
ATGGCAAGATTAGTCATGGTTGGTTTGGTCTTTGCAAGTGTATCATTGCTCCTGTCATCTGCAGCAGCTCAAGTTCCTGATTATGACTATCTACCCGATGAGTCCATTACCCGTACTAGCTTTGAAGATGATGAAGCCTACGCTCCAGTTACGATCGAGTATGGGTTTCCACCAGAGCCGTTCCGTGGATATTATGACAATTTAGACCAATGTGAAGACAACGTCAACCTAAATTGTGTTAAAGAGATTTACCGCGGTATGTTCAAAACCGAGCCTCTTAATAAGAACTGTTGCAAGAACCTTGTGCAGATGGGATATGGCTGTCACAGTAGGTTAGCGAAACTTGTGAACCCGAGAAATAAAGCAAAAGCTTCCATGGCATTGGCTAAGAGTGTGCAGATTTGGCAAAAGTGTGTCTCAGTTGTTGAAACTGGTCGATTCCCTGCTGTTCCTGCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

154

Amino Acids

17.2

Weight (kDa)

5.84

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 70 - 139 7.1e-11 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 255
AciI CCGC 2 cut(s) 253, 255
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 3 cut(s) 125, 265, 428
AluBI AGCT 3 cut(s) 68, 120, 371
AluI AGCT 3 cut(s) 68, 120, 371
Alw26I GTCTC 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 352
ApeKI GCWGC 2 cut(s) 62, 65
AvaI CYCGRG 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 74, 77
BccI CCATC 1 cut(s) 304
BceAI ACGGC 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 421
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 60
BisI GCNGC 2 cut(s) 63, 66
BlsI GCNGC 2 cut(s) 64, 67
BmeT110I CYCGRG 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 184, 253, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 146, 436
Bse3DI GCAATG 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 184, 253, 375
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMI GCAATG 1 cut(s) 44
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 2 cut(s) 146, 436
BseXI GCAGC 2 cut(s) 74, 77
BsgI GTGCAG 2 cut(s) 326, 416
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 352
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 421
BsoBI CYCGRG 1 cut(s) 352
Bsp143I GATC 1 cut(s) 153
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 2 cut(s) 253, 255
BspCNI CTCAG 1 cut(s) 431
BspFNI CGCG 1 cut(s) 255
BspMAI CTGCAG 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 44
BsrI ACTGG 2 cut(s) 146, 436
BssECI CCNNGG 3 cut(s) 184, 253, 375
BssMI GATC 1 cut(s) 153
BssT1I CCWWGG 1 cut(s) 375
Bst4CI ACNGT 2 cut(s) 290, 329
BstDEI CTNAG 2 cut(s) 387, 418
BstDSI CCRYGG 3 cut(s) 184, 253, 375
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 1 cut(s) 153
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 3 cut(s) 377, 403, 452
BstSFI CTRYAG 1 cut(s) 60
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 2 cut(s) 74, 77
BstV2I GAAGAC 1 cut(s) 222
BstXI CCANNNNNNTGG 1 cut(s) 382
BtgI CCRYGG 3 cut(s) 184, 253, 375
Cfr42I CCGCGG 1 cut(s) 256
Csp6I GTAC 1 cut(s) 114
CviAII CATG 2 cut(s) 16, 376
CviJI RGCY 8 cut(s) 68, 120, 137, 178, 274, 321, 371, 386
CviKI_1 RGCY 8 cut(s) 68, 120, 137, 178, 274, 321, 371, 386
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 2 cut(s) 387, 418
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 2 cut(s) 19, 379
FaiI YATR 7 cut(s) 17, 84, 162, 195, 260, 318, 377
FatI CATG 2 cut(s) 15, 375
Fnu4HI GCNGC 2 cut(s) 63, 66
Fsp4HI GCNGC 2 cut(s) 63, 66
FspBI CTAG 1 cut(s) 117
GluI GCNGC 2 cut(s) 63, 66
GsuI CTGGAG 1 cut(s) 129
Hin1II CATG 2 cut(s) 19, 379
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 369
HinfI GANTC 2 cut(s) 101, 438
Hpy166II GTNNAC 2 cut(s) 226, 349
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 2 cut(s) 226, 349
HpyCH4III ACNGT 2 cut(s) 290, 329
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 35, 62, 294, 307, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 403, 452
HpyF3I CTNAG 2 cut(s) 387, 418
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 2 cut(s) 19, 379
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 3 cut(s) 54, 148, 460
LpnPI CCDG 6 cut(s) 65, 90, 159, 186, 417, 456
Lsp1109I GCAGC 2 cut(s) 74, 77
MaeI CTAG 1 cut(s) 117
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 148, 323
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 2 cut(s) 137, 227
MluCI AATT 2 cut(s) 199, 232
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 1 cut(s) 285
MseI TTAA 2 cut(s) 240, 279
MspA1I CMGCKG 1 cut(s) 255
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 3 cut(s) 377, 403, 452
NcoI CCATGG 1 cut(s) 375
NdeII GATC 1 cut(s) 153
NlaIII CATG 2 cut(s) 19, 379
NmuCI GTSAC 1 cut(s) 323
PfeI GAWTC 1 cut(s) 438
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 2 cut(s) 64, 67
Ple19I CGATCG 1 cut(s) 156
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PstI CTGCAG 1 cut(s) 64
PsyI GACNNNGTC 1 cut(s) 221
PvuI CGATCG 1 cut(s) 156
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SacII CCGCGG 1 cut(s) 256
SaqAI TTAA 2 cut(s) 240, 279
SatI GCNGC 2 cut(s) 63, 66
Sau3AI GATC 1 cut(s) 153
SchI GAGTC 1 cut(s) 110
SetI ASST 7 cut(s) 70, 122, 225, 231, 303, 335, 373
SfcI CTRYAG 1 cut(s) 60
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 2 cut(s) 199, 232
SsiI CCGC 2 cut(s) 253, 255
SspMI CTAG 1 cut(s) 117
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 2 cut(s) 290, 329
TaiI ACGT 1 cut(s) 225
TaqI TCGA 2 cut(s) 156, 436
TasI AATT 2 cut(s) 199, 232
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 2 cut(s) 240, 279
Tru9I TTAA 2 cut(s) 240, 279
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 2 cut(s) 62, 65
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 1 cut(s) 147
TspGWI ACGGA 1 cut(s) 173
Tth111I GACNNNGTC 1 cut(s) 221
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.