RLG00000002208

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000474
Physical Location & Seq
Reverse (-)
29588119 .. 29588598
480 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002208

Sequence Viewer

Length: 480 bp
ATGGCTAGAGTTGATCAAAATGTGTTTGTCAAGGTAGGTTTGGTGTTAGTAAGTGTAGCATTATTCATGTCATCATTTGCAGTAGCTCAAGAATATGAGGTTATTTATCAAGTTTCTGAGGATGCTGCGGCGAGCCCTATTGGCATCGAGGTTGACTATTTAGTCATTGATGATTCTTCAGAAAACGGTCAATATGCGTTCCCTCCTAACCTATCACCGGAATCTTATAAGAAGTTGGATGAATGTGTAAATAAGATGACAGGATATTATGCTGAAGAGATCTTGGCCCGAATTTTTAAAAGCGAACCTCTCACTACGGATAACTGCAAAGCCCTTCTGCATTTGGGATATGATTGCCACATTCGGTCAGTGAAAACTCTGCTCTCACGTCCGGAACTAAAAGAAAAAGCTTCTGAGATACTGCCTAAAAGTGTGCAGATTTGGGAAACATGTGCCTTTGTTGCCCCTTCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

160

Amino Acids

17.73

Weight (kDa)

4.74

Isoelectric Point (pI)

65.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 81 - 151 1.1e-06 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AasI GACNNNNNNGTC 1 cut(s) 161
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 291
AcuI CTGAAG 2 cut(s) 162, 294
AfiI CCNNNNNNNGG 1 cut(s) 217
AflIII ACRYGT 1 cut(s) 449
AjiI CACGTC 1 cut(s) 389
AluBI AGCT 2 cut(s) 86, 410
AluI AGCT 2 cut(s) 86, 410
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 286
AsuHPI GGTGA 1 cut(s) 207
BanII GRGCYC 1 cut(s) 137
BbvI GCAGC 1 cut(s) 112
BclI TGATCA 1 cut(s) 13
BfaI CTAG 1 cut(s) 6
BglI GCCNNNNNGGC 1 cut(s) 141
BglII AGATCT 1 cut(s) 279
BisI GCNGC 2 cut(s) 126, 129
BlsI GCNGC 2 cut(s) 127, 130
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 286
BmsI GCATC 2 cut(s) 112, 153
BpuEI CTTGAG 1 cut(s) 72
BsaWI WCCGGW 2 cut(s) 217, 391
Bsc4I CCNNNNNNNGG 1 cut(s) 217
BseAI TCCGGA 1 cut(s) 391
BseGI GGATG 2 cut(s) 127, 244
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 2 cut(s) 108, 405
BseXI GCAGC 1 cut(s) 112
BsgI GTGCAG 1 cut(s) 455
BshFI GGCC 1 cut(s) 287
BsiSI CCGG 2 cut(s) 218, 392
BslI CCNNNNNNNGG 1 cut(s) 217
BsnI GGCC 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 137
Bsp13I TCCGGA 1 cut(s) 391
Bsp143I GATC 2 cut(s) 13, 279
BspACI CCGC 1 cut(s) 128
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 2 cut(s) 109, 406
BspEI TCCGGA 1 cut(s) 391
BssMI GATC 2 cut(s) 13, 279
Bst4CI ACNGT 1 cut(s) 188
Bst6I CTCTTC 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 133
BstDEI CTNAG 2 cut(s) 117, 414
BstF5I GGATG 2 cut(s) 127, 244
BstKTI GATC 2 cut(s) 16, 282
BstMBI GATC 2 cut(s) 13, 279
BstMWI GCNNNNNNNGC 2 cut(s) 141, 461
BstNSI RCATGY 1 cut(s) 453
BstV1I GCAGC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BsuRI GGCC 1 cut(s) 287
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 2 cut(s) 127, 244
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 1 cut(s) 133
Cfr13I GGNCC 1 cut(s) 286
CviAII CATG 2 cut(s) 67, 450
CviJI RGCY 6 cut(s) 5, 86, 135, 287, 332, 410
CviKI_1 RGCY 6 cut(s) 5, 86, 135, 287, 332, 410
DdeI CTNAG 2 cut(s) 117, 414
DpnI GATC 2 cut(s) 15, 281
DpnII GATC 2 cut(s) 13, 279
DraI TTTAAA 1 cut(s) 298
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 162, 294
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 2 cut(s) 70, 453
FaiI YATR 7 cut(s) 68, 96, 195, 228, 270, 351, 451
FatI CATG 2 cut(s) 66, 449
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 2 cut(s) 126, 129
FokI GGATG 2 cut(s) 134, 251
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 2 cut(s) 126, 129
FspBI CTAG 1 cut(s) 6
GluI GCNGC 2 cut(s) 126, 129
HaeIII GGCC 1 cut(s) 287
HapII CCGG 2 cut(s) 218, 392
Hin1II CATG 2 cut(s) 70, 453
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 2 cut(s) 173, 221
HpaII CCGG 2 cut(s) 218, 392
HphI GGTGA 1 cut(s) 207
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 3 cut(s) 118, 181, 415
Hpy188III TCNNGA 2 cut(s) 89, 392
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 344, 477
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 4 cut(s) 80, 327, 340, 436
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 461
HpyF3I CTNAG 2 cut(s) 117, 414
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 2 cut(s) 70, 453
Kpn2I TCCGGA 1 cut(s) 391
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 2 cut(s) 13, 279
LpnPI CCDG 3 cut(s) 231, 246, 405
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 2 cut(s) 112, 153
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 388
MalI GATC 2 cut(s) 15, 281
MboI GATC 2 cut(s) 13, 279
MboII GAAGA 2 cut(s) 168, 287
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 137
MluCI AATT 1 cut(s) 291
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 5 cut(s) 91, 112, 142, 213, 318
MroI TCCGGA 1 cut(s) 391
MseI TTAA 1 cut(s) 297
MspI CCGG 2 cut(s) 218, 392
MwoI GCNNNNNNNGC 2 cut(s) 141, 461
NdeII GATC 2 cut(s) 13, 279
NlaIII CATG 2 cut(s) 70, 453
NspI RCATGY 1 cut(s) 453
PciI ACATGT 1 cut(s) 449
PfeI GAWTC 2 cut(s) 173, 221
PkrI GCNGC 2 cut(s) 127, 130
PscI ACATGT 1 cut(s) 449
PsiI TTATAA 1 cut(s) 228
PspPI GGNCC 1 cut(s) 286
PsuI RGATCY 1 cut(s) 279
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 2 cut(s) 126, 129
Sau3AI GATC 2 cut(s) 13, 279
Sau96I GGNCC 1 cut(s) 286
SduI GDGCHC 1 cut(s) 137
SetI ASST 9 cut(s) 36, 40, 88, 102, 153, 213, 310, 391, 412
SfaNI GCATC 2 cut(s) 112, 153
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 1 cut(s) 291
SsiI CCGC 1 cut(s) 128
SspMI CTAG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 188
TaiI ACGT 1 cut(s) 391
TaqI TCGA 1 cut(s) 147
TaqII GACCGA 1 cut(s) 354
TasI AATT 1 cut(s) 291
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 2 cut(s) 173, 221
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 1 cut(s) 375
TseI GCWGC 1 cut(s) 125
TspDTI ATGAA 2 cut(s) 55, 255
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 1 cut(s) 375
XapI RAATTY 1 cut(s) 291
XceI RCATGY 1 cut(s) 453
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.