Rh5DG513400

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000474
Physical Location & Seq
Forward (+)
80000180 .. 80002525
2346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG513400.1

Sequence Viewer

Length: 645 bp
ATGGCAAGATTTGTCATGGTTGGTTTGGTCTGTGCAAGTGTATCATTGCTCATGTCATCTGCAGCAGCTCAAGTTCCTGATTATGACTATCTACCCGATGAGTCCATTACCCCTGCTAGCTTTGAAGATGATGAAGCCTACGCTCCAGTTACGATCGAGTATGGGTTTCCACCAGAGCCATTCCGTGGATATTATGACAATTTAGACCAATGTGAAGACAACTTCAACCTAAATTGTGTTAAAGAGATTTACCGCGGTATGTTCAAAACCGAGCCTCTTAATAAGAACTGTTGCAAGAACCTTGTGCAGATGGGATATGGTTGTCACAGTAGGTTAGCAAAATTTGTGAACCCGAGAAATAAAGAAAAAGCTTCCATGGCATTGGCTAAGAGTCTGCAGATTTGGCAAAAATGTGTCTCAGTTGTTGAAACTGGTCGATTCCCCGCTAAGAAACTTGTGAAGAAAGTAACCTCAGGAGGAGAAGCCTGCTCTTCTTCACTCTTAACCTTCAGCTCTTGTTCATTCGCACTTCCTTTCTCTTCCGCCGTTGTTGACACACAACTGACACAAGATTCAAATGGGGGAGAGAATCAGAGAGTGAATGGGGAAAGATCTTTGAAGGTGAAAGATGAAGCTATAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

214

Amino Acids

23.62

Weight (kDa)

5.57

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 70 - 139 8e-11 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 185
AccII CGCG 1 cut(s) 255
AciI CCGC 4 cut(s) 253, 255, 444, 543
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 493
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 6 cut(s) 125, 226, 265, 428, 576, 619
AluBI AGCT 5 cut(s) 68, 120, 371, 513, 635
AluI AGCT 5 cut(s) 68, 120, 371, 513, 635
Alw26I GTCTC 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 352
ApeKI GCWGC 2 cut(s) 62, 65
ApoI RAATTY 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 634
AsuNHI GCTAGC 1 cut(s) 116
AvaI CYCGRG 1 cut(s) 352
AxyI CCTNAGG 1 cut(s) 472
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 74, 77
BccI CCATC 1 cut(s) 304
BceAI ACGGC 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 421
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 2 cut(s) 60, 395
BglII AGATCT 1 cut(s) 611
BisI GCNGC 2 cut(s) 63, 66
BlsI GCNGC 2 cut(s) 64, 67
BmeT110I CYCGRG 1 cut(s) 352
BmtI GCTAGC 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 184, 253, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 146, 436
Bse21I CCTNAGG 1 cut(s) 472
Bse3DI GCAATG 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 184, 253, 375
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMI GCAATG 1 cut(s) 44
BseMII CTCAG 2 cut(s) 432, 486
BseNI ACTGG 2 cut(s) 146, 436
BseRI GAGGAG 1 cut(s) 492
BseXI GCAGC 2 cut(s) 74, 77
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 352
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 421
BsoBI CYCGRG 1 cut(s) 352
Bsp143I GATC 2 cut(s) 153, 611
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 4 cut(s) 253, 255, 444, 543
BspCNI CTCAG 2 cut(s) 431, 485
BspFNI CGCG 1 cut(s) 255
BspMAI CTGCAG 2 cut(s) 64, 399
BspOI GCTAGC 1 cut(s) 120
BspQI GCTCTTC 1 cut(s) 496
BsrDI GCAATG 1 cut(s) 44
BsrI ACTGG 2 cut(s) 146, 436
BssECI CCNNGG 3 cut(s) 184, 253, 375
BssMI GATC 2 cut(s) 153, 611
BssT1I CCWWGG 1 cut(s) 375
Bst4CI ACNGT 2 cut(s) 290, 329
Bst6I CTCTTC 2 cut(s) 496, 544
BstC8I GCNNGC 2 cut(s) 118, 487
BstDEI CTNAG 4 cut(s) 387, 418, 447, 472
BstDSI CCRYGG 3 cut(s) 184, 253, 375
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 2 cut(s) 156, 614
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 2 cut(s) 153, 611
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 377, 403
BstSFI CTRYAG 2 cut(s) 60, 395
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 2 cut(s) 74, 77
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 611
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 1 cut(s) 611
Bsu36I CCTNAGG 1 cut(s) 472
BtgI CCRYGG 3 cut(s) 184, 253, 375
Cac8I GCNNGC 2 cut(s) 118, 487
Cfr42I CCGCGG 1 cut(s) 256
CviAII CATG 3 cut(s) 16, 52, 376
DdeI CTNAG 4 cut(s) 387, 418, 447, 472
DpnI GATC 2 cut(s) 155, 613
DpnII GATC 2 cut(s) 153, 611
Eam1104I CTCTTC 2 cut(s) 496, 544
EarI CTCTTC 2 cut(s) 496, 544
EciI GGCGGA 1 cut(s) 532
Eco130I CCWWGG 1 cut(s) 375
Eco57I CTGAAG 1 cut(s) 493
Eco81I CCTNAGG 1 cut(s) 472
Eco88I CYCGRG 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 3 cut(s) 19, 55, 379
FaiI YATR 9 cut(s) 17, 53, 84, 162, 195, 260, 318, 377, 638
FatI CATG 3 cut(s) 15, 51, 375
FauI CCCGC 1 cut(s) 451
Fnu4HI GCNGC 2 cut(s) 63, 66
Fsp4HI GCNGC 2 cut(s) 63, 66
FspBI CTAG 1 cut(s) 117
GluI GCNGC 2 cut(s) 63, 66
GsuI CTGGAG 1 cut(s) 129
Hin1II CATG 3 cut(s) 19, 55, 379
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HindIII AAGCTT 1 cut(s) 369
HinfI GANTC 5 cut(s) 101, 391, 438, 572, 589
HphI GGTGA 1 cut(s) 634
Hpy166II GTNNAC 2 cut(s) 349, 553
Hpy188I TCNGA 1 cut(s) 594
Hpy188III TCNNGA 2 cut(s) 77, 474
Hpy8I GTNNAC 2 cut(s) 349, 553
HpyAV CCTTC 2 cut(s) 517, 613
HpyCH4III ACNGT 2 cut(s) 290, 329
HpyCH4V TGCA 5 cut(s) 35, 62, 294, 307, 397
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 403
HpyF3I CTNAG 4 cut(s) 387, 418, 447, 472
Hsp92II CATG 3 cut(s) 19, 55, 379
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 2 cut(s) 153, 611
LguI GCTCTTC 1 cut(s) 496
LmnI GCTCC 1 cut(s) 148
LpnPI CCDG 7 cut(s) 90, 126, 159, 186, 417, 459, 499
Lsp1109I GCAGC 2 cut(s) 74, 77
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 3 cut(s) 148, 323, 466
MalI GATC 2 cut(s) 155, 613
MboI GATC 2 cut(s) 153, 611
MboII GAAGA 6 cut(s) 137, 227, 472, 483, 486, 531
MflI RGATCY 1 cut(s) 611
MluCI AATT 3 cut(s) 199, 232, 341
MlyI GAGTC 2 cut(s) 110, 400
MnlI CCTC 3 cut(s) 285, 470, 481
MseI TTAA 3 cut(s) 240, 279, 503
MspA1I CMGCKG 1 cut(s) 255
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 2 cut(s) 377, 403
NcoI CCATGG 1 cut(s) 375
NdeII GATC 2 cut(s) 153, 611
NheI GCTAGC 1 cut(s) 116
NlaIII CATG 3 cut(s) 19, 55, 379
NmuCI GTSAC 1 cut(s) 323
PciSI GCTCTTC 1 cut(s) 496
PfeI GAWTC 3 cut(s) 438, 572, 589
PflMI CCANNNNNTGG 1 cut(s) 185
PkrI GCNGC 2 cut(s) 64, 67
Ple19I CGATCG 1 cut(s) 156
PleI GAGTC 2 cut(s) 109, 399
PpsI GAGTC 2 cut(s) 109, 399
PstI CTGCAG 2 cut(s) 64, 399
PsuI RGATCY 1 cut(s) 611
PvuI CGATCG 1 cut(s) 156
SacII CCGCGG 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 496
SaqAI TTAA 3 cut(s) 240, 279, 503
SatI GCNGC 2 cut(s) 63, 66
Sau3AI GATC 2 cut(s) 153, 611
SchI GAGTC 2 cut(s) 110, 400
SfcI CTRYAG 2 cut(s) 60, 395
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 3 cut(s) 199, 232, 341
SsiI CCGC 4 cut(s) 253, 255, 444, 543
SspMI CTAG 1 cut(s) 117
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 2 cut(s) 290, 329
TaqI TCGA 2 cut(s) 156, 436
TasI AATT 3 cut(s) 199, 232, 341
TfiI GAWTC 3 cut(s) 438, 572, 589
Tru1I TTAA 3 cut(s) 240, 279, 503
Tru9I TTAA 3 cut(s) 240, 279, 503
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 2 cut(s) 62, 65
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 3 cut(s) 147, 510, 645
TspGWI ACGGA 1 cut(s) 173
Van91I CCANNNNNTGG 1 cut(s) 185
XapI RAATTY 1 cut(s) 341
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.