MD13G1274700.v1.1

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000474
Physical Location & Seq
Forward (+)
37366842 .. 37367369
528 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1274700.v1.1.491

Sequence Viewer

Length: 528 bp
ATGTCAAATTATATCAGCAGCATCTTGTTCATGGTTATTACCACCTTCTTATTAGGCTTGCTTTCACCGGCAGCTGTACTTGCATCTAGAGACATCTCACCACCTTATTACGAGTCTAATTATGATCCTGATTTGGCTCCTGAAGAAGCCCCGATAATCGTCAAGGACTTTCCACCGGAGCCACTGCCCGGTCGGGACTCTGATCAGTCTAACTTCTTGGAAGCATGTGCTGTTCGACTAACAGGGGATTGTGGGGAAGCAATTTTCACTGATATCTTTTGGAACCAAACTAGCCCTATCAGTGACCATTGCTGCCGGAATCTTGTGGGGTTAGGACTTCGCTGCCACGTTGGTTTGGTGGCTAATATAATAAATAACCACCCTGCAGCCTTCAAAACGAATGAGATCATGCTTCTTTTGAGAAGCCTGGACATCTGGAATATGTGTGCTTTGGTTGCAAAAGTTGAGCCCGCTATCCCTTCTCCCTCCCCACATGAAGAAAATAATGAGATTGTTTTCTACGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

176

Amino Acids

19.31

Weight (kDa)

4.48

Isoelectric Point (pI)

65.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 76 - 149 5e-10 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 471
AclWI GGATC 1 cut(s) 119
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 1 cut(s) 78
AfiI CCNNNNNNNGG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 394
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 1 cut(s) 74
AluI AGCT 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 84
AlwI GGATC 1 cut(s) 119
ApeKI GCWGC 5 cut(s) 18, 71, 312, 342, 386
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 2 cut(s) 57, 90
BanII GRGCYC 1 cut(s) 471
BbvI GCAGC 5 cut(s) 30, 83, 299, 329, 398
BciT130I CCWGG 1 cut(s) 428
BclI TGATCA 1 cut(s) 202
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 84
BfaI CTAG 2 cut(s) 87, 291
BfmI CTRYAG 1 cut(s) 384
BisI GCNGC 5 cut(s) 19, 72, 313, 343, 387
BlsI GCNGC 5 cut(s) 20, 73, 314, 344, 388
Bme1390I CCNGG 2 cut(s) 189, 428
BmiI GGNNCC 3 cut(s) 138, 180, 284
BmrFI CCNGG 2 cut(s) 189, 428
BmsI GCATC 2 cut(s) 30, 92
BpuMI CCSGG 1 cut(s) 189
BsaAI YACGTR 1 cut(s) 523
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 1 cut(s) 67
Bse3DI GCAATG 1 cut(s) 307
BseBI CCWGG 1 cut(s) 428
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMI GCAATG 1 cut(s) 307
BseXI GCAGC 5 cut(s) 30, 83, 299, 329, 398
Bsh1285I CGRYCG 1 cut(s) 193
BsiEI CGRYCG 1 cut(s) 193
BsiSI CCGG 4 cut(s) 68, 176, 189, 316
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 3 cut(s) 124, 202, 405
BspACI CCGC 1 cut(s) 471
BspLI GGNNCC 3 cut(s) 138, 180, 284
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 307
BsrFI RCCGGY 1 cut(s) 67
BssAI RCCGGY 1 cut(s) 67
BssMI GATC 3 cut(s) 124, 202, 405
Bst2UI CCWGG 1 cut(s) 428
BstBAI YACGTR 1 cut(s) 523
BstC8I GCNNGC 2 cut(s) 59, 471
BstKTI GATC 3 cut(s) 127, 205, 408
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 3 cut(s) 124, 202, 405
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 2 cut(s) 80, 455
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 1 cut(s) 228
BstSCI CCNGG 2 cut(s) 187, 426
BstSFI CTRYAG 1 cut(s) 384
BstSNI TACGTA 1 cut(s) 523
BstV1I GCAGC 5 cut(s) 30, 83, 299, 329, 398
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 3 cut(s) 182, 267, 307
Cac8I GCNNGC 2 cut(s) 59, 471
Cfr10I RCCGGY 1 cut(s) 67
Csp6I GTAC 1 cut(s) 77
CviAII CATG 4 cut(s) 31, 225, 409, 494
CviQI GTAC 1 cut(s) 77
DpnI GATC 3 cut(s) 126, 204, 407
DpnII GATC 3 cut(s) 124, 202, 405
Eco105I TACGTA 1 cut(s) 523
Eco24I GRGCYC 1 cut(s) 471
Eco32I GATATC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 426
EcoRV GATATC 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 471
FaeI CATG 4 cut(s) 34, 228, 412, 497
FaiI YATR 9 cut(s) 12, 32, 123, 226, 368, 410, 443, 495, 526
FaqI GGGAC 1 cut(s) 209
FatI CATG 4 cut(s) 30, 224, 408, 493
FauI CCCGC 1 cut(s) 478
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 5 cut(s) 19, 72, 313, 343, 387
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 5 cut(s) 19, 72, 313, 343, 387
FspBI CTAG 2 cut(s) 87, 291
GluI GCNGC 5 cut(s) 19, 72, 313, 343, 387
HapII CCGG 4 cut(s) 68, 176, 189, 316
Hin1II CATG 4 cut(s) 34, 228, 412, 497
HinfI GANTC 3 cut(s) 113, 197, 319
HpaII CCGG 4 cut(s) 68, 176, 189, 316
HphI GGTGA 2 cut(s) 57, 90
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 5 cut(s) 87, 128, 140, 194, 436
HpyAV CCTTC 3 cut(s) 55, 400, 489
HpyCH4IV ACGT 2 cut(s) 348, 522
HpyCH4V TGCA 3 cut(s) 83, 386, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 455
HpySE526I ACGT 2 cut(s) 348, 522
Hsp92II CATG 4 cut(s) 34, 228, 412, 497
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 3 cut(s) 124, 202, 405
LmnI GCTCC 2 cut(s) 142, 178
Lsp1109I GCAGC 5 cut(s) 30, 83, 299, 329, 398
LweI GCATC 2 cut(s) 30, 92
MaeI CTAG 2 cut(s) 87, 291
MaeII ACGT 2 cut(s) 348, 522
MaeIII GTNAC 1 cut(s) 302
MalI GATC 3 cut(s) 126, 204, 407
MboI GATC 3 cut(s) 124, 202, 405
MboII GAAGA 2 cut(s) 155, 509
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 3 cut(s) 7, 118, 261
MlyI GAGTC 2 cut(s) 122, 191
MnlI CCTC 1 cut(s) 496
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 4 cut(s) 68, 176, 189, 316
MspR9I CCNGG 2 cut(s) 189, 428
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 2 cut(s) 80, 455
NciI CCSGG 1 cut(s) 189
NdeII GATC 3 cut(s) 124, 202, 405
NlaIII CATG 4 cut(s) 34, 228, 412, 497
NlaIV GGNNCC 3 cut(s) 138, 180, 284
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 1 cut(s) 228
PfeI GAWTC 1 cut(s) 319
PkrI GCNGC 5 cut(s) 20, 73, 314, 344, 388
PleI GAGTC 2 cut(s) 121, 191
PpsI GAGTC 2 cut(s) 121, 191
Ppu21I YACGTR 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 3 cut(s) 138, 180, 284
PstI CTGCAG 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 78
RsaNI GTAC 1 cut(s) 77
SatI GCNGC 5 cut(s) 19, 72, 313, 343, 387
Sau3AI GATC 3 cut(s) 124, 202, 405
SchI GAGTC 2 cut(s) 122, 191
ScrFI CCNGG 2 cut(s) 189, 428
SduI GDGCHC 1 cut(s) 471
SetI ASST 5 cut(s) 47, 76, 106, 351, 525
SfaNI GCATC 2 cut(s) 30, 92
SfcI CTRYAG 1 cut(s) 384
SnaBI TACGTA 1 cut(s) 523
Sse9I AATT 3 cut(s) 7, 118, 261
SsiI CCGC 1 cut(s) 471
SspMI CTAG 2 cut(s) 87, 291
StyD4I CCNGG 2 cut(s) 187, 426
TaiI ACGT 2 cut(s) 351, 525
TaqI TCGA 1 cut(s) 235
TasI AATT 3 cut(s) 7, 118, 261
TatI WGTACW 1 cut(s) 76
TfiI GAWTC 1 cut(s) 319
TscAI CASTG 3 cut(s) 189, 274, 307
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 5 cut(s) 18, 71, 312, 342, 386
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 2 cut(s) 19, 510
TspRI CASTG 3 cut(s) 189, 274, 307
XbaI TCTAGA 1 cut(s) 86
XceI RCATGY 1 cut(s) 228
XspI CTAG 2 cut(s) 87, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.