MD16G1259400.v1.1

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000474
Physical Location & Seq
Reverse (-)
31615631 .. 31616080
450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1259400.v1.1.491

Sequence Viewer

Length: 450 bp
ATGCCGGGGTTGGTGTTAGCAGCAATCATCGTCGTGTCATCGGCGAGACTTTCATCTGCATCTGAAGACGTTTATGGAAATTATGCTGATCGTGATGAACCATCATCAATAAGCCCTAGCCCTAATCCTAGCCCTAGCCCTAGCCCTAGCACCACAGAAGATATCAGCAGCAGCAGCAGTAATTTCCCAGATGTGCTGCCGCCGGAGCCCTCCCAGGGATACTACAGGCATTTGAGGAAATGGGCAGACGAAATAAGCCCTCTGTGCGCTGAACAGATCTTTGAGGGTATGTTCTACAGCAACGAAGAACTGACTGAGGATTGTTGCGCAAACCTTTTGCATGTGGGATACGAATGTCACCGGGTGTTGGTTAAGCTAATCCTCCTAGAACCGACGTTCAAAGGCAAAGCTTCCGAGGCATTGCACAAGAGTTTCCAAATATGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

150

Amino Acids

16.38

Weight (kDa)

4.68

Isoelectric Point (pI)

75.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 87 - 144 1e-09 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 328
AciI CCGC 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 84
AdeI CACNNNGTG 1 cut(s) 364
AfiI CCNNNNNNNGG 2 cut(s) 215, 367
AgsI TTSAA 1 cut(s) 400
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 3 cut(s) 376, 410, 447
AluI AGCT 3 cut(s) 376, 410, 447
Alw26I GTCTC 1 cut(s) 40
ApeKI GCWGC 5 cut(s) 20, 168, 171, 174, 196
AspLEI GCGC 2 cut(s) 269, 329
AsuC2I CCSGG 2 cut(s) 6, 362
AsuHPI GGTGA 1 cut(s) 350
BaeI ACNNNNGTAYC 2 cut(s) 340, 373
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 5 cut(s) 32, 180, 183, 183, 186
BccI CCATC 1 cut(s) 109
BciT130I CCWGG 1 cut(s) 215
BciVI GTATCC 2 cut(s) 212, 341
BcnI CCSGG 2 cut(s) 6, 362
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 7 cut(s) 117, 129, 135, 141, 147, 386, 448
BfmI CTRYAG 2 cut(s) 223, 295
BfuI GTATCC 2 cut(s) 212, 341
BglII AGATCT 1 cut(s) 276
BisI GCNGC 6 cut(s) 21, 169, 172, 175, 197, 200
BlsI GCNGC 6 cut(s) 22, 170, 173, 176, 198, 201
Bme1390I CCNGG 3 cut(s) 6, 215, 362
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 3 cut(s) 6, 215, 362
BmsI GCATC 1 cut(s) 68
BpiI GAAGAC 1 cut(s) 72
BpuMI CCSGG 2 cut(s) 6, 362
BsaJI CCNNGG 4 cut(s) 5, 213, 214, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 367
Bse3DI GCAATG 1 cut(s) 419
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 4 cut(s) 5, 213, 214, 414
BseLI CCNNNNNNNGG 2 cut(s) 215, 367
BseMI GCAATG 1 cut(s) 419
BseMII CTCAG 1 cut(s) 306
BseXI GCAGC 5 cut(s) 32, 180, 183, 183, 186
BsiSI CCGG 3 cut(s) 5, 203, 361
BslI CCNNNNNNNGG 2 cut(s) 215, 367
BsmAI GTCTC 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 88, 276
BspACI CCGC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 307
BspLI GGNNCC 1 cut(s) 207
BsrDI GCAATG 1 cut(s) 419
BssECI CCNNGG 4 cut(s) 5, 213, 214, 414
BssMI GATC 2 cut(s) 88, 276
Bst2UI CCWGG 1 cut(s) 215
BstDEI CTNAG 1 cut(s) 315
BstHHI GCGC 2 cut(s) 269, 329
BstKTI GATC 2 cut(s) 91, 279
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 2 cut(s) 88, 276
BstMWI GCNNNNNNNGC 4 cut(s) 174, 205, 264, 416
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 3 cut(s) 4, 213, 360
BstSFI CTRYAG 2 cut(s) 223, 295
BstV1I GCAGC 5 cut(s) 32, 180, 183, 183, 186
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 276
BstYI RGATCY 1 cut(s) 276
BsuI GTATCC 2 cut(s) 212, 341
CfoI GCGC 2 cut(s) 269, 329
CviAII CATG 1 cut(s) 341
DdeI CTNAG 1 cut(s) 315
DpnI GATC 2 cut(s) 90, 278
DpnII GATC 2 cut(s) 88, 276
DraIII CACNNNGTG 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 210
Eco32I GATATC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 213
EcoRV GATATC 1 cut(s) 163
EcoT38I GRGCYC 1 cut(s) 210
FaeI CATG 1 cut(s) 344
FaiI YATR 5 cut(s) 75, 84, 290, 342, 442
FatI CATG 1 cut(s) 340
Fnu4HI GCNGC 6 cut(s) 21, 169, 172, 175, 197, 200
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 6 cut(s) 21, 169, 172, 175, 197, 200
FspBI CTAG 7 cut(s) 117, 129, 135, 141, 147, 386, 448
FspI TGCGCA 1 cut(s) 328
GlaI GCGC 2 cut(s) 268, 328
GluI GCNGC 6 cut(s) 21, 169, 172, 175, 197, 200
HapII CCGG 3 cut(s) 5, 203, 361
HhaI GCGC 2 cut(s) 269, 329
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 2 cut(s) 267, 327
HinP1I GCGC 2 cut(s) 267, 327
HindIII AAGCTT 1 cut(s) 408
HpaII CCGG 3 cut(s) 5, 203, 361
HphI GGTGA 1 cut(s) 350
Hpy188I TCNGA 2 cut(s) 64, 415
Hpy188III TCNNGA 1 cut(s) 92
Hpy99I CGWCG 2 cut(s) 35, 397
HpyCH4IV ACGT 2 cut(s) 69, 395
HpyCH4V TGCA 3 cut(s) 59, 340, 424
HpyF10VI GCNNNNNNNGC 4 cut(s) 174, 205, 264, 416
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 2 cut(s) 69, 395
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 2 cut(s) 267, 327
Kzo9I GATC 2 cut(s) 88, 276
LmnI GCTCC 2 cut(s) 205, 444
LpnPI CCDG 7 cut(s) 18, 200, 201, 211, 216, 227, 374
Lsp1109I GCAGC 5 cut(s) 32, 180, 183, 183, 186
LweI GCATC 1 cut(s) 68
MaeI CTAG 7 cut(s) 117, 129, 135, 141, 147, 386, 448
MaeII ACGT 2 cut(s) 69, 395
MaeIII GTNAC 1 cut(s) 356
MalI GATC 2 cut(s) 90, 278
MboI GATC 2 cut(s) 88, 276
MboII GAAGA 3 cut(s) 77, 170, 317
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 2 cut(s) 79, 181
MnlI CCTC 7 cut(s) 220, 228, 270, 277, 310, 392, 409
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 32
MspI CCGG 3 cut(s) 5, 203, 361
MspR9I CCNGG 3 cut(s) 6, 215, 362
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 4 cut(s) 174, 205, 264, 416
NciI CCSGG 2 cut(s) 6, 362
NdeII GATC 2 cut(s) 88, 276
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 356
NsbI TGCGCA 1 cut(s) 328
NspI RCATGY 1 cut(s) 344
PasI CCCWGGG 1 cut(s) 214
PkrI GCNGC 6 cut(s) 22, 170, 173, 176, 198, 201
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 207
PsuI RGATCY 1 cut(s) 276
RseI CAYNNNNRTG 1 cut(s) 32
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 6 cut(s) 21, 169, 172, 175, 197, 200
Sau3AI GATC 2 cut(s) 88, 276
ScrFI CCNGG 3 cut(s) 6, 215, 362
SduI GDGCHC 1 cut(s) 210
SetI ASST 6 cut(s) 72, 336, 378, 398, 412, 449
SfaNI GCATC 1 cut(s) 68
SfcI CTRYAG 2 cut(s) 223, 295
SmiMI CAYNNNNRTG 1 cut(s) 32
Sse9I AATT 2 cut(s) 79, 181
SsiI CCGC 1 cut(s) 200
SspMI CTAG 7 cut(s) 117, 129, 135, 141, 147, 386, 448
StyD4I CCNGG 3 cut(s) 4, 213, 360
TaiI ACGT 2 cut(s) 72, 398
TasI AATT 2 cut(s) 79, 181
TauI GCSGC 1 cut(s) 202
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 5 cut(s) 20, 168, 171, 174, 196
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 2 cut(s) 42, 111
XceI RCATGY 1 cut(s) 344
XspI CTAG 7 cut(s) 117, 129, 135, 141, 147, 386, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.