Rroxscaffold_1G00007950

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000474
Physical Location & Seq
Reverse (-)
10151990 .. 10152418
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00007950.1

Sequence Viewer

Length: 429 bp
ATGACATCATTTGCAGCAGCTCAAGAATATGAGCTTATTTATCAAGTTTCTGAAGATGCTGCGGCGAGCCCTATTGGCATCGAGGTTGACTATTTTGTCATTGATGATGCCTTCCCTCCAATTTCTTCAGAAAACGGTCAATATGCGTTCCTTCCTAAGCTATCACCGGAATCTTATAAGAAGTTGGATGAATGTGTAAATAAGATTACAGAATATTATGCTGGAGAGATCTTGCCCAACATTTTTAAAAGCGAACCTCTCACTACGGATAATTGCAAAGCTGTTCTGCATTTGGGATATGATTGTCACATCCGATCAGTGAAAACTCTGCTCTCACGTCCGGAACTAAAAGAAAAGGCTTCAGAGATACTGCCTAAAAGTGTGCAGATTTGGGAAACATGTGCCTTTGTTGCCGCTTCTCCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

142

Amino Acids

15.86

Weight (kDa)

4.63

Isoelectric Point (pI)

66.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 64 - 134 1.7e-06 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AasI GACNNNNNNGTC 1 cut(s) 95
AccIII TCCGGA 1 cut(s) 340
AciI CCGC 2 cut(s) 62, 414
AcuI CTGAAG 3 cut(s) 72, 111, 345
AflIII ACRYGT 1 cut(s) 398
AjiI CACGTC 1 cut(s) 338
AluBI AGCT 4 cut(s) 20, 34, 160, 281
AluI AGCT 4 cut(s) 20, 34, 160, 281
Aor13HI TCCGGA 1 cut(s) 340
ApeKI GCWGC 3 cut(s) 14, 17, 59
AsuHPI GGTGA 1 cut(s) 156
BanII GRGCYC 1 cut(s) 71
BbvI GCAGC 3 cut(s) 26, 29, 46
BfaI CTAG 1 cut(s) 427
BglI GCCNNNNNGGC 1 cut(s) 75
BglII AGATCT 1 cut(s) 228
BisI GCNGC 5 cut(s) 15, 18, 60, 63, 414
BlsI GCNGC 5 cut(s) 16, 19, 61, 64, 415
BmgBI CACGTC 1 cut(s) 338
BmsI GCATC 3 cut(s) 46, 87, 97
BpmI CTGGAG 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 6
BsaWI WCCGGW 2 cut(s) 166, 340
BseAI TCCGGA 1 cut(s) 340
BseGI GGATG 2 cut(s) 193, 309
BseXI GCAGC 3 cut(s) 26, 29, 46
BsgI GTGCAG 1 cut(s) 404
BsiSI CCGG 2 cut(s) 167, 341
Bsp1286I GDGCHC 1 cut(s) 71
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 2 cut(s) 228, 314
BspACI CCGC 2 cut(s) 62, 414
BspEI TCCGGA 1 cut(s) 340
BssMI GATC 2 cut(s) 228, 314
Bst4CI ACNGT 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 1 cut(s) 156
BstF5I GGATG 2 cut(s) 193, 309
BstKTI GATC 2 cut(s) 231, 317
BstMBI GATC 2 cut(s) 228, 314
BstMWI GCNNNNNNNGC 2 cut(s) 75, 410
BstNSI RCATGY 1 cut(s) 402
BstV1I GCAGC 3 cut(s) 26, 29, 46
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
BtrI CACGTC 1 cut(s) 338
BtsCI GGATG 2 cut(s) 193, 309
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 67
CviAII CATG 1 cut(s) 399
CviJI RGCY 6 cut(s) 20, 34, 69, 160, 281, 359
CviKI_1 RGCY 6 cut(s) 20, 34, 69, 160, 281, 359
DdeI CTNAG 1 cut(s) 156
DpnI GATC 2 cut(s) 230, 316
DpnII GATC 2 cut(s) 228, 314
DraI TTTAAA 1 cut(s) 247
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 71
Eco57I CTGAAG 3 cut(s) 72, 111, 345
EcoT38I GRGCYC 1 cut(s) 71
FaeI CATG 1 cut(s) 402
FaiI YATR 6 cut(s) 30, 144, 177, 219, 300, 400
FatI CATG 1 cut(s) 398
Fnu4HI GCNGC 5 cut(s) 15, 18, 60, 63, 414
FokI GGATG 2 cut(s) 200, 296
FriOI GRGCYC 1 cut(s) 71
Fsp4HI GCNGC 5 cut(s) 15, 18, 60, 63, 414
FspBI CTAG 1 cut(s) 427
GluI GCNGC 5 cut(s) 15, 18, 60, 63, 414
GsuI CTGGAG 1 cut(s) 243
HapII CCGG 2 cut(s) 167, 341
Hin1II CATG 1 cut(s) 402
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HinfI GANTC 1 cut(s) 170
HpaII CCGG 2 cut(s) 167, 341
HphI GGTGA 1 cut(s) 156
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 4 cut(s) 52, 130, 314, 364
Hpy188III TCNNGA 2 cut(s) 23, 341
Hpy8I GTNNAC 1 cut(s) 88
HpyAV CCTTC 2 cut(s) 121, 161
HpyCH4III ACNGT 1 cut(s) 137
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 4 cut(s) 14, 276, 289, 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 410
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 1 cut(s) 402
Kpn2I TCCGGA 1 cut(s) 340
Kzo9I GATC 2 cut(s) 228, 314
LpnPI CCDG 3 cut(s) 180, 207, 354
Lsp1109I GCAGC 3 cut(s) 26, 29, 46
LweI GCATC 3 cut(s) 46, 87, 97
MaeI CTAG 1 cut(s) 427
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 1 cut(s) 305
MalI GATC 2 cut(s) 230, 316
MboI GATC 2 cut(s) 228, 314
MboII GAAGA 2 cut(s) 65, 117
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 2 cut(s) 120, 271
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 3 cut(s) 76, 126, 267
MroI TCCGGA 1 cut(s) 340
MseI TTAA 1 cut(s) 246
MspI CCGG 2 cut(s) 167, 341
MwoI GCNNNNNNNGC 2 cut(s) 75, 410
NdeII GATC 2 cut(s) 228, 314
NlaIII CATG 1 cut(s) 402
NmuCI GTSAC 1 cut(s) 305
NspI RCATGY 1 cut(s) 402
PciI ACATGT 1 cut(s) 398
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 5 cut(s) 16, 19, 61, 64, 415
PscI ACATGT 1 cut(s) 398
PsiI TTATAA 1 cut(s) 177
PsuI RGATCY 1 cut(s) 228
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 5 cut(s) 15, 18, 60, 63, 414
Sau3AI GATC 2 cut(s) 228, 314
SduI GDGCHC 1 cut(s) 71
SetI ASST 7 cut(s) 22, 36, 87, 162, 259, 283, 340
SfaNI GCATC 3 cut(s) 46, 87, 97
SmlI CTYRAG 1 cut(s) 21
SmoI CTYRAG 1 cut(s) 21
Sse9I AATT 2 cut(s) 120, 271
SsiI CCGC 2 cut(s) 62, 414
SspI AATATT 1 cut(s) 215
SspMI CTAG 1 cut(s) 427
TaaI ACNGT 1 cut(s) 137
TaiI ACGT 1 cut(s) 340
TaqI TCGA 1 cut(s) 81
TasI AATT 2 cut(s) 120, 271
TauI GCSGC 2 cut(s) 65, 416
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 3 cut(s) 14, 17, 59
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 1 cut(s) 204
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 1 cut(s) 324
XceI RCATGY 1 cut(s) 402
XspI CTAG 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.