AT2G21727

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000474
Physical Location & Seq
Reverse (-)
9278567 .. 9279080
514 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21727.1

Sequence Viewer

Length: 456 bp
ATGGAGAATAAAACCATGTTCATGATATTTTCTTCGATCATGGTTTTGCTATCGTTTTCTCATCTAACTTTGGCCAAAGAAAGAGGTAATGACGAGCCACTACTCATTTCAGACGACGAGTTTGATGCAATGATGGCAAGGTCACCGGCATCAGACGATTATAACGACAATATGTTACGTAAATATTCAGAAAAAGAAAAAGATTATCTTAAGAACTGCGGTAAAAAGATGGACATGCCATATGGCCCATATCAATGTGCGGACGAAGTGATAGCTTATATTGTTCAGAACAAAAGTGTTTCAAGGGTTTGTTGTTGGGGGATAGTGAAAGCTGGAAAGGAATGCCATAAGAAATGGACGGGATTATTTTTTGAAATGTATCAACTCAAACGTTTTAGTTCTAAGAAGTTTTCCAAAACTAATGAAATATGGAACATGTGTTCTACTGATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

151

Amino Acids

17.63

Weight (kDa)

7.54

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 73 - 148 9.6e-15 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 162
AciI CCGC 2 cut(s) 219, 260
AclI AACGTT 1 cut(s) 391
AcoI YGGCCR 1 cut(s) 72
AflII CTTAAG 1 cut(s) 209
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 2 cut(s) 303, 374
AloI GAACNNNNNNTCC 2 cut(s) 424, 456
AluBI AGCT 2 cut(s) 275, 332
AluI AGCT 2 cut(s) 275, 332
AoxI GGCC 2 cut(s) 72, 244
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AspS9I GGNCC 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 135
BalI TGGCCA 1 cut(s) 74
BccI CCATC 2 cut(s) 127, 223
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BfrI CTTAAG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 245
BmsI GCATC 2 cut(s) 115, 158
BsaAI YACGTR 1 cut(s) 179
Bse118I RCCGGY 1 cut(s) 145
Bse3DI GCAATG 1 cut(s) 135
BseMI GCAATG 1 cut(s) 135
BshFI GGCC 2 cut(s) 74, 246
BsiSI CCGG 1 cut(s) 146
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 2 cut(s) 74, 246
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 2 cut(s) 219, 260
BspANI GGCC 2 cut(s) 74, 246
BspHI TCATGA 1 cut(s) 21
BspTI CTTAAG 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 145
BssMI GATC 1 cut(s) 36
BstAFI CTTAAG 1 cut(s) 209
BstBAI YACGTR 1 cut(s) 179
BstDEI CTNAG 1 cut(s) 402
BstEII GGTNACC 1 cut(s) 141
BstKTI GATC 1 cut(s) 39
BstMBI GATC 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNSI RCATGY 2 cut(s) 238, 439
BstPI GGTNACC 1 cut(s) 141
BstSNI TACGTA 1 cut(s) 179
BsuRI GGCC 2 cut(s) 74, 246
CciI TCATGA 1 cut(s) 21
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 245
CviAII CATG 5 cut(s) 16, 22, 40, 235, 436
CviJI RGCY 5 cut(s) 74, 97, 246, 275, 332
CviKI_1 RGCY 5 cut(s) 74, 97, 246, 275, 332
DdeI CTNAG 1 cut(s) 402
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
EaeI YGGCCR 1 cut(s) 72
Eco105I TACGTA 1 cut(s) 179
Eco91I GGTNACC 1 cut(s) 141
EcoO65I GGTNACC 1 cut(s) 141
FaeI CATG 5 cut(s) 19, 25, 43, 238, 439
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FatI CATG 5 cut(s) 15, 21, 39, 234, 435
FauNDI CATATG 1 cut(s) 241
HaeIII GGCC 2 cut(s) 74, 246
HapII CCGG 1 cut(s) 146
Hin1II CATG 5 cut(s) 19, 25, 43, 238, 439
HpaII CCGG 1 cut(s) 146
HphI GGTGA 1 cut(s) 135
Hpy188I TCNGA 4 cut(s) 112, 154, 190, 288
Hpy188III TCNNGA 1 cut(s) 22
Hpy99I CGWCG 1 cut(s) 119
HpyCH4IV ACGT 2 cut(s) 178, 391
HpyCH4V TGCA 1 cut(s) 128
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 2 cut(s) 178, 391
Hsp92II CATG 5 cut(s) 19, 25, 43, 238, 439
Kzo9I GATC 1 cut(s) 36
LpnPI CCDG 2 cut(s) 159, 318
LweI GCATC 2 cut(s) 115, 158
MaeII ACGT 2 cut(s) 178, 391
MaeIII GTNAC 2 cut(s) 141, 174
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 1 cut(s) 24
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 1 cut(s) 451
MluNI TGGCCA 1 cut(s) 74
MnlI CCTC 1 cut(s) 77
Mox20I TGGCCA 1 cut(s) 74
MscI TGGCCA 1 cut(s) 74
MseI TTAA 2 cut(s) 210, 454
MslI CAYNNNNRTG 2 cut(s) 20, 253
Msp20I TGGCCA 1 cut(s) 74
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 347
MwoI GCNNNNNNNGC 1 cut(s) 134
NdeI CATATG 1 cut(s) 241
NdeII GATC 1 cut(s) 36
NlaIII CATG 5 cut(s) 19, 25, 43, 238, 439
NmuCI GTSAC 1 cut(s) 141
NspI RCATGY 2 cut(s) 238, 439
PagI TCATGA 1 cut(s) 21
PciI ACATGT 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 347
Ppu21I YACGTR 1 cut(s) 179
PscI ACATGT 1 cut(s) 435
PsiI TTATAA 1 cut(s) 162
Psp1406I AACGTT 1 cut(s) 391
PspEI GGTNACC 1 cut(s) 141
PspPI GGNCC 1 cut(s) 245
RseI CAYNNNNRTG 2 cut(s) 20, 253
SaqAI TTAA 2 cut(s) 210, 454
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 1 cut(s) 245
SetI ASST 6 cut(s) 88, 143, 181, 277, 334, 394
SfaNI GCATC 2 cut(s) 115, 158
SmiMI CAYNNNNRTG 2 cut(s) 20, 253
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SnaBI TACGTA 1 cut(s) 179
Sse9I AATT 1 cut(s) 451
SsiI CCGC 2 cut(s) 219, 260
SspI AATATT 1 cut(s) 185
TaiI ACGT 2 cut(s) 181, 394
TaqI TCGA 1 cut(s) 35
TasI AATT 1 cut(s) 451
Tru1I TTAA 2 cut(s) 210, 454
Tru9I TTAA 2 cut(s) 210, 454
TseFI GTSAC 1 cut(s) 141
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 2 cut(s) 10, 438
Vha464I CTTAAG 1 cut(s) 209
XceI RCATGY 2 cut(s) 238, 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.